CN214622674U

CN214622674U - Elisa plate positioning device

Info

Publication number: CN214622674U
Application number: CN202120564578.5U
Authority: CN
Inventors: 张文娟; 时长军; 戴静
Original assignee: Suzhou Xishan Biotechnology Co ltd
Current assignee: Suzhou Xishan Biotechnology Co ltd
Priority date: 2021-03-19
Filing date: 2021-03-19
Publication date: 2021-11-05
Anticipated expiration: 2031-03-19

Abstract

The utility model discloses an ELIAS plate positioner, it includes base, stand, goes up framework and positioning mechanism, is equipped with ELIAS plate location portion on the base, the lower extreme and the base fixed connection of stand, the upper end and the last framework fixed connection of stand, go up the framework setting directly over the base, positioning mechanism including fixing two sets of X slide rails and two sets of Y slide rails in last framework, X slide rail parallel arrangement, two sets of Y slide rail parallel arrangement all are equipped with portable buckle on every X slide rail and Y slide rail, all are equipped with the multichannel wire casing on X slide rail and Y slide rail, be connected with two first lines parallel with the Y slide rail between two portable buckles on two X slide rails, be connected with two second lines parallel with the X slide rail between two portable buckles on two Y slide rails. The utility model discloses an ELIAS plate positioner can prevent ELIAS plate application of sample mistake, improves application of sample efficiency, can carry out the result reading recheck of certain hole site during the experiment reading fast, guarantees that the reading is accurate.

Description

Elisa plate positioning device

Technical Field

The utility model belongs to biological assay or detection area, concretely relates to ELIAS plate positioner.

Background

The ELISA plate is an important carrier in enzyme-linked immunosorbent (ELISA) experiments, the conventional 96-hole ELISA plate is widely applied to the field of medical examination, known antigens or antibodies are adsorbed on the surface of a solid-phase ELISA plate, after a sample to be detected is added, the antibodies in the sample and coating antigens are specifically combined, non-combined substances are removed by washing, and then labeled secondary antibodies of enzyme are added to form an antigen-antibody-enzyme complex with the labeled secondary antibodies; adding a substrate chromogenic reagent of the enzyme for reaction and color development, and finally determining whether the antibody or the antigen exists in the sample to be detected by naked eye interpretation or scanning of an enzyme labeling instrument.

The conventional 96-well ELISA plate is labor-intensive for ELISA of multiple projects and multiple ELISA plates simultaneously, especially, in the sample adding step, an operator needs to manually add samples into each hole in the microplate in a one-to-one correspondence manner, the operation process is very complicated and is easy to make mistakes, and the too long sample adding time can cause the incubation time difference between different micropores to be large, so that the final detection result is inaccurate or wrong. The utility model patent document with publication number CN207472893U discloses an ELIAS plate auxiliary operation device for supplementary to the ELIAS plate application of sample operation, prevent that the hourglass in the application of sample experimentation from dripping the wrong phenomenon, ensure the accuracy of porous application of sample simultaneously, improve the operating efficiency of application of sample. The utility model discloses a utility model patent document that publication number is CN212255356U discloses an ELIAS plate with application of sample scale, an ELIAS plate with application of sample scale is provided, its structure includes the ELIAS plate and installs the application of sample scale on the ELIAS plate, the application of sample scale includes horizontal scale and vertical scale, can adjust the position of scale according to actual need, the operating efficiency is high, the problem of easily obscuring when having solved the downthehole application of sample to the ELIAS plate, the demand of the quick accurate application of sample of ELIAS plate has been satisfied, but the defect of this patent is firstly scale and the integrative increase cost of manufacture of ELIAS plate, secondly two scales are opaque, quick application of sample and reading rechecking process can't be realized to its alternately mesopore.

The 384-hole enzyme label plate has high flux, can concentrate a plurality of detection items on one antigen plate for continuous detection, has less usage amount of samples and reagents, saves labor, and is the development direction of future enzyme-linked immunosorbent assay. However, the 384-well ELISA plate has many well sites, small hole spacing, narrow frame, unclear transparent character-lining marks, row marks of "A-P" and column marks of "1-24" on the frame, and easy dislocation of sample-adding process and result reading, which limits the use of the 384-well ELISA plate.

SUMMERY OF THE UTILITY MODEL

The utility model aims at overcoming the 384-hole ELIAS plate above-mentioned not enough, providing a 384 ELIAS plate positioner, carrying out ELIAS plate appearance and reading recheck in-process, utilize this ELIAS plate 384 ELIAS plate positioner can be accurate location to ranks or the hole site that the operation corresponds, improve operating efficiency greatly, prevent the application of sample mistake from appearing, improve the efficiency of result reading recheck.

The technical scheme of the utility model as follows: an ELISA plate positioning device comprises a base, a column, an upper frame body and a positioning mechanism, wherein an ELISA plate positioning part is arranged on the base, the lower end of the column is fixedly connected with the base, the upper end of the column is fixedly connected with the upper frame body, the upper frame body is arranged right above the base, an opening corresponding to an ELISA plate on the base is arranged in the middle of the upper frame body, the positioning mechanism comprises an X slide rail and a Y slide rail which are fixed on the upper frame body, the X slide rail and the Y slide rail are respectively provided with two groups, the two groups of X slide rails are arranged in parallel, the two groups of Y slide rails are arranged in parallel, a movable buckle is arranged on each X slide rail and each Y slide rail, a plurality of wire grooves are arranged on the X slide rail and each Y slide rail, the positions of the wire grooves on the X slide rail correspond to the positions of each row of the ELISA plate, the positions of the wire grooves on the Y slide rail correspond to the positions of each row of the ELISA plate, two movable buckles on the two X slide rails are connected with a first connecting wire parallel to the Y slide rail, the distance between the first connecting lines corresponds to the length of the sample adding holes in the ELISA plate, two second connecting lines parallel to the X slide rails are connected between two movable buckles on the two Y slide rails, the distance between the second connecting lines corresponds to the width of the sample adding holes in the ELISA plate, multiple rows and multiple columns of sample adding holes are formed in the ELISA plate, the first connecting lines can be coincided with one row of sample adding holes in the ELISA plate, and the second connecting lines can be coincided with one column of sample adding holes in the ELISA plate.

The further proposal is that the upper surface of the upper frame body or the position close to the upper surface in the upper frame body is provided with a row mark and a column mark, and the row mark and the column mark respectively correspond to the sample adding holes of each row and each column of the ELISA plate. The sample adding of the ELISA plate is more convenient by arranging the row marks and the column marks.

The further scheme is that magnetic drawing strips are arranged beside an X slide rail and a Y slide rail on the upper frame body, a magnetic pen is arranged on the movable buckle, and when the movable buckle moves along the X slide rail or the Y slide rail, the magnetic pen can draw lines with corresponding colors on the magnetic drawing strips and record the moving area of the movable buckle. Through setting up magnetism drawing board strip, when making the application of sample of ELIAS plate, the region of having removed forms the lines on the magnetism drawing board strip, marks the region of application of sample, avoids the application of sample mistake.

The further scheme is that a color removing piece is arranged below the magnetic drawing board strip below the upper frame body, and lines drawn on the magnetic drawing board strip by the magnetic pen can be removed when the color removing piece moves below the magnetic drawing board strip. Through setting up the piece that goes wrong colour, the last application of sample of ELIAS plate is accomplished one line or a back, can get rid of the lines on the magnetism drawing board strip to follow-up application of sample to next line or next line, perhaps after the completion of whole applications of sample, get rid of the lines of drawing on the magnetism drawing board strip.

The further scheme is that the positioning part of the ELISA plate is provided with a rectangular groove, the size of the rectangular groove corresponds to that of the ELISA plate, a clamping piece is arranged in the rectangular groove, and the ELISA plate is fixed on the base through the clamping piece. Through the arrangement, the positioning part of the ELISA plate can be stably fixed on the base.

The base and the upper frame body can be kept parallel through the arrangement, so that the positioning mechanism can more accurately position each sample adding hole on the ELISA plate.

The further scheme is that the X slide rail and the Y slide rail are vertically arranged, and the X slide rail and the Y slide rail are arranged in a rectangular shape. Through the arrangement, the movable buckle can slide more smoothly on the X slide rail and the Y slide rail, and the positioning mechanism can more accurately position the sample adding hole on the ELISA plate.

The utility model has the advantages that: 1) the positioning device of the ELIAS plate of the utility model is provided with the positioning part of the ELIAS plate on the base, and the ELIAS plate positioning part can be stably fixed on the base by the clamping piece, thereby being convenient for the sample adding operation; 2) the utility model discloses an ELIAS plate positioning device, set up X slide rail and Y slide rail on the upper frame, and set up portable buckle on X slide rail and Y slide rail, set up the line mark and the column mark that correspond with the application of sample hole of ELIAS plate on X slide rail and Y slide rail, connect with the double-link between the portable buckle on two X slide rails or two Y slide rails, the area between the double-link is the blank area, through this setting, it is high to solve the scheme that sets up the scale on the ELIAS plate in the prior art, the scale is opaque, can't see ELIAS plate application of sample hole, the problem such as the quick application of sample and reading rechecking process can't be realized to the middle hole that intersects, when the portable buckle of the utility model moves, the area between the double-link just in time can correspond with a line or a column of application of sample hole of ELIAS plate, make the ELIAS plate can carry out porous application of sample simultaneously, application of sample efficiency and accuracy are higher, and can avoid repeated sample adding or missing sample adding and other sample adding errors; 3) the utility model discloses an ELIAS plate positioner, when the application of sample, form the lines on the magnetism picture strip in the region that the application of sample was crossed, avoid the repeated application of sample; 4) the utility model discloses an ELIAS plate positioner both can be according to the application of sample in line, also can be according to the application of sample that lists, and the application of sample is more nimble, and the experimenter can carry out the application of sample according to the custom, and the efficiency and the accuracy of application of sample are higher. 5) The utility model discloses an ELIAS plate positioner can fix a position a certain positive reaction hole rapidly at the recheck in-process of result reading, and the quick interpretation of experimental result improves reading efficiency and accuracy.

Drawings

Fig. 1 is a schematic structural diagram of the microplate positioning device of the present invention.

Fig. 2 is a schematic structural diagram of the upper frame body and the positioning mechanism of the microplate positioning device of the present invention.

Fig. 3 is a schematic structural diagram of the base of the microplate positioning device of the present invention.

Labeled as: the base 1, the rectangle recess 11, chucking spare 12, stand 2, go up frame 3, the line mark 31, the column mark 32, magnetism picture lath 33, decoloration piece 34, positioning mechanism 4, X slide rail 41, Y slide rail 42, portable buckle 43, wire casing 44, first line 45, second line 46, magnetic pen 47.

Detailed Description

The invention is further described with reference to the following figures and examples.

Fig. 1-3 show a positioning device for an elisa plate of the present invention, which comprises a base 1, a column 2, an upper frame 3 and a positioning mechanism 4.

The base 1 is the pedestal that is used for fixed ELIAS plate and support upper frame body and positioning mechanism, and base 1 is integrated into one piece or components of a whole that can function independently sub-assembly, and base 1's shape is rectangle or circular or other shapes, and the size of base will be greater than the ELIAS plate size. The middle part of the base 1 is provided with an ELISA plate positioning part for fixing the ELISA plate. ELIAS plate location portion can carry out spacing fixed to the ELIAS plate through multiple mode, for example fix a position through bellied four corners location fixture block, fix through the kicking block, perhaps fix through the briquetting and so on locate mode. The utility model discloses an ELIAS plate location portion is equipped with the rectangle recess 11 that is used for fixed ELIAS plate, and the width and the length of rectangle recess 11 are unanimous with the width and the length of standard ELIAS plate, adopt the rectangle recess to carry on spacingly to the ELIAS plate. Be equipped with chucking spare 12 in rectangle recess 11, chucking spare 12 is located the apex position of rectangle recess 11, and chucking spare 12 carries out spacing fixed to the apex position of ELIAS plate. For example, a microplate of 384 wells has 16 rows and 24 columns, and has a length of 127mm, a width of 8.5mm, and a depth of 3mm, corresponding to the dimensions of the rectangular grooves.

The vertical setting of stand 2, the lower extreme and the base 1 fixed connection of stand 2, the upper end and the last framework 3 fixed connection of stand 2. The quantity of stand 2 is according to base 1 and the nimble setting of the connection needs of last framework 3, in order to make base 1 and the connection of last framework 3 more firm, can set up stand 2 in the four corners position of base 1.

Go up framework 3 and be the framework of rectangle or other shapes, be equipped with the opening that corresponds with the ELIAS plate on the base 1 in the middle part of last framework 3, the opening is the rectangle opening, and the opening is greater than the ELIAS plate size.

The positioning mechanism 4 comprises an X slide rail 41 and a Y slide rail 42 which are fixed on the upper frame body 3, two groups of X slide rails 41 and two groups of Y slide rails 42 are respectively arranged, the two groups of X slide rails 41 are arranged in parallel, the two groups of Y slide rails 42 are arranged in parallel, a movable buckle 443 is arranged on each X slide rail 41 and each Y slide rail 42, a plurality of wire grooves 44 are respectively arranged on the X slide rails 41 and the Y slide rails 42, the positions of the wire grooves 44 on the X slide rails 41 correspond to the positions of the rows of the microplate, the positions of the wire grooves 44 on the Y slide rails 42 correspond to the positions of the columns of the microplate, the movable buckles 43 can be positioned at the positions corresponding to the wire grooves 44, two first connecting lines 45 which are parallel to the Y slide rails 42 are connected between the two movable buckles 43 on the two X slide rails 41, the distance between the first connecting lines 45 corresponds to the length of the sample adding holes on the microplate, two movable buckles on the two Y slide rails 42 are connected with two second connecting lines 46 which are parallel to the X slide rails, the distance between the second connecting lines 46 corresponds to the width of a sample adding hole on the ELISA plate, and the first connecting line 45 and the second connecting line 46 are metal lines, nylon lines, plastic lines or other material lines; and a plurality of rows and columns of sample adding holes are formed in the ELISA plate, the first connecting line can be coincided with one row of sample adding holes of the ELISA plate, and the second connecting line can be coincided with one column of sample adding holes of the ELISA plate.

On the upper surface of the upper frame body 3 or in the position close to the upper surface in the upper frame body 3, a row mark 31 and a column mark 32 marked by striking colors are arranged, for the ELIAS plate with 384 empty spaces, the columns are marked as 1-24 columns, the rows are marked as A-P rows, the row mark 31 is arranged beside an X slide rail 41, and the column mark 32 is arranged beside a Y slide rail 42, thus the problem that the character marks on the ELIAS plate with 384 holes are unclear can be solved.

Magnetic picture strip strips 33 are arranged beside an X slide rail 41 and a Y slide rail 42 on the upper frame body 3, a magnetic pen 47 is arranged on the movable buckle, and when the movable buckle 43 moves along the X slide rail 41 or the Y slide rail 42, the magnetic pen 47 can draw lines with corresponding colors on the magnetic picture strip 33 and record the area moved by the movable buckle 43. Through setting up magnetism drawing board strip 33, when making the enzyme label board application of sample, the region of having removed forms the lines on the magnetism drawing board strip 33, marks the region of application of sample, avoids the application of sample mistake.

The lower part of the upper frame 3 is provided with a color removing member 34 below the magnetic picture strip 33, and the color removing member 34 can remove lines drawn on the magnetic picture strip 33 by the magnetic pen 47 when moving below the magnetic picture strip 33. Through setting up the piece 34 that goes look, the last application of sample of ELIAS plate is accomplished one line or a back, can get rid of the lines on the magnetism picture lath 33 to follow-up application of sample to next line or next line, perhaps after the whole application of sample is accomplished, get rid of the lines that draw on the magnetism picture lath 33.

The utility model discloses an ELIAS plate positioner, when using, on putting the ELIAS plate fixing base on the base with the 384 hole ELIAS plate, through chucking spare to the spacing fixed of 384 hole ELIAS plate, prevent rocking of ELIAS plate. When feeding materials, the movable buckles on the Y slide rail can be fixed, the movable buckles on the X slide rail are moved, and the first connecting line between the two movable buckles moves along the Y slide rail direction, so that the sample feeding according to rows is realized; or the movable buckle on the X slide rail is fixed, and the movable buckle on the Y slide rail is moved, so that the second connecting line between the two movable buckles moves along the direction of the X slide rail, and the sample adding in rows is realized; and the movable buckles on the X slide rail and the Y slide rail can be moved simultaneously, and the intersection part of the first connecting line and the second connecting line is positioned to the appointed sample adding hole on the ELISA plate for positioning sample adding. When the application of sample, when portable buckle removed along X slide rail or Y slide rail, the magnetic pen on portable buckle painted striking colour mark line on the magnetism drawing board strip in the upper frame body, and the mark application of sample is accomplished, and the application of sample of accomplishing the application of sample of a line or a row or on the application of sample hole of whole application of sample board back, through going the color slide under the upper frame body, can get rid of the colour of magnetism drawing board strip to the application of sample next time. Go up the row mark that marks with striking colour and mark more clearly with row in the framework, look over when the application of sample of being convenient for, prevent the application of sample mistake.

The above description is only for the preferred embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes and substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention should be covered by the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. An ELIAS plate positioner which characterized in that: the positioning mechanism comprises an X slide rail and a Y slide rail which are fixed on the upper frame body, wherein the X slide rail and the Y slide rail are respectively provided with two groups, the two groups of X slide rails are arranged in parallel, the two groups of Y slide rails are arranged in parallel, each X slide rail and each Y slide rail are provided with a movable buckle, the X slide rail and the Y slide rail are both provided with a plurality of wire grooves, the positions of the wire grooves on the X slide rail correspond to the positions of the rows of the ELISA plate, the positions of the wire grooves on the Y slide rail correspond to the positions of the columns of the ELISA plate, two first connecting lines which are parallel to the Y slide rail are connected between the two movable buckles on the two X slide rails, the distance between the first connecting lines corresponds to the length of the sample adding holes in the ELISA plate, two second connecting lines parallel to the X slide rails are connected between two movable buckles on the two Y slide rails, the distance between the second connecting lines corresponds to the width of the sample adding holes in the ELISA plate, multiple rows and multiple columns of sample adding holes are formed in the ELISA plate, the first connecting lines can be coincided with one row of sample adding holes in the ELISA plate, and the second connecting lines can be coincided with one column of sample adding holes in the ELISA plate.

2. The microplate positioning device of claim 1, wherein: and the upper surface of the upper frame body or the position close to the upper surface in the upper frame body is provided with a row mark and a column mark, and the row mark and the column mark respectively correspond to the sample adding holes in each row and each column of the ELISA plate.

3. The microplate positioning device of claim 1, wherein: the magnetic drawing board strip is arranged beside the X slide rail and the Y slide rail on the upper frame body, the magnetic pen is arranged on the movable buckle, when the movable buckle moves along the X slide rail or the Y slide rail, the magnetic pen can draw lines with corresponding colors on the magnetic drawing board strip, and the area moved by the movable buckle is recorded.

4. The microplate positioning device of claim 3, wherein: the lower part of the upper frame body is provided with a color removing piece below the magnetic drawing board strip, and the color removing piece can remove lines drawn on the magnetic drawing board strip by the magnetic pen when moving below the magnetic drawing board strip.

5. The microplate positioning device of claim 1, wherein: the ELIAS plate location portion be equipped with the rectangle recess, the size and the ELIAS plate of rectangle recess are corresponding, are equipped with the chucking spare in the rectangle recess, the ELIAS plate passes through the chucking spare to be fixed on the base.

6. The microplate positioning device of claim 1, wherein: the upright posts are respectively and vertically arranged with the base and the upper frame body.

7. The microplate positioning device of claim 1, wherein: the X slide rail and the Y slide rail are vertically arranged, and the X slide rail and the Y slide rail are arranged in a rectangular shape.

8. The microplate positioning device of claim 1, wherein: the ELISA plate is a 384-hole ELISA plate.