CN213274458U

CN213274458U - Gene sequencing kit detecting system

Info

Publication number: CN213274458U
Application number: CN202022055847.3U
Authority: CN
Inventors: 郭素; 贾亚坤; 张君霞
Original assignee: Sina Biotechnology Beijing Co ltd
Current assignee: Sina Biotechnology Beijing Co ltd
Priority date: 2020-09-18
Filing date: 2020-09-18
Publication date: 2021-05-25
Anticipated expiration: 2030-09-18

Abstract

The utility model relates to a gene sequencing kit detecting system belongs to external diagnostic reagent field. The utility model discloses mainly used detects the multiple reagent filling volume of special-shaped kit, mainly realizes through following mode: a gene sequencing kit detection system comprises a kit full detection caliper, a light source and a gene sequencing kit; the full-detection caliper is characterized in that the full-detection caliper of the kit comprises a horizontal strip, a vertical strip and a handle; the vertical strip is provided with positioning scales; the horizontal strips have the function of stable planar placement and fixation; the vertical strip is used for comparing with the liquid level of the kit and judging the liquid loading amount of the kit; the up and down position of the light source can be moved and aligned with each of the separate reagent compartments in the reagent cartridge.

Description

Gene sequencing kit detecting system

Technical Field

The utility model relates to a gene sequencing kit detecting system belongs to external diagnostic reagent field, concretely relates to sequencing field of next generation sequencing kit.

Background

Gene sequencing is a novel gene detection technology, and can analyze and determine gene sequences from blood or human appendages to predict the possibility of suffering from various diseases, such as cancers or leukemia. Products and techniques related to gene sequencing have evolved from laboratory studies to clinical use. Although the first generation DNA sequencing technology helps people to complete a lot of sequencing work with its sequencing read length up to 1000bp and high accuracy of 99.999%, its testing speed is slow, cost is high, flux is low, and so on, so it cannot be applied to the popularization. With the progress of scientific technology and the development of sequencing technology by researchers, the 454 sequencing system published by Roche corporation in 2005 marks the era of cross-human high-throughput parallel sequencing of sequencing technology. The second generation DNA sequencing technology is also called Massive Parallel Sequencing (MPS) and high-throughput sequencing (HTS), and can perform rapid sequencing analysis on hundreds of thousands of DNA molecules in hundreds of samples and thousands of samples at 1 time with low cost and accuracy of 99% or more. Typical techniques in this period include 454 by Roche, Solexa by Illumina, SOLID by ABI, and the like. The core idea behind second generation Sequencing technologies is Sequencing by Synthesis (Sequencing by Sequencing), i.e., the Sequencing of DNA by capturing the tags of newly synthesized ends. The second generation sequencing technology greatly reduces the sequencing cost, greatly improves the sequencing speed and keeps high accuracy. Meanwhile, the kit matched with the existing second-generation sequencing technology has a relatively complex structure, and for some special-shaped kits, the defect of high quality inspection difficulty may exist. The utility model discloses a detecting system of gene sequencing kit for whether detect reagent box is qualified.

Disclosure of Invention

The utility model aims at providing a simple structure, convenient to use, the full detecting system of universality to the problem of part dysmorphism kit quality testing difficulty.

The utility model discloses mainly used detects multiple reagent filling volume of special-shaped kit, its principle is that turn into the liquid level height in specific container with the filling volume, then through the height relative position of the marking height that detects special slide caliper rule and filling liquid level, judges whether every kind of reagent filling volume reaches the requirement. The method is mainly realized by the following steps:

a gene sequencing kit detection system is characterized by comprising a kit full detection caliper, a light source and a gene sequencing kit; the full-detection caliper is characterized in that the full-detection caliper of the kit comprises a horizontal strip, a vertical strip and a handle; the vertical strip is provided with positioning scales; the horizontal strips have the function of stable planar placement and fixation; the vertical strip is used for comparing with the liquid level of the kit and judging the liquid loading amount of the kit; the up and down position of the light source can be moved and aligned with each of the separate reagent compartments in the reagent cartridge.

According to a preferred embodiment, the number of light sources is the same as the number of individually separated reagent compartments in the kit that need to be detected.

According to a preferred embodiment, the full caliper of the kit and the light source are fixed on the same base.

According to a preferred embodiment, the width of the vertical strips is 1-5 cm.

According to a preferred embodiment, said vertical strips are opaque.

According to a preferred embodiment, the device further comprises a background plate; the vertical strips are transparent, the light source is a focusing light source, and the projection of the transparent vertical strips is projected onto the background plate.

According to a preferred embodiment, the kit has 4 separate reagent compartments, and the horizontal strip has 4 reagent compartments.

According to a preferred embodiment, the light source is a laser light source, and the spot size of the laser is 0.2-3 mm.

The utility model discloses a gene sequencing kit detecting system utilizes the light source and examines the volume of the dress liquid of slide caliper rule affirmation kit entirely whether qualified. The device has the advantages of simple structure, convenient use, no pollution and high detection precision, and is beneficial to saving the labor and equipment cost required by quality inspection on a reagent production line; meanwhile, the utility model is a non-destructive inspection, which can not cause product loss after quality inspection; additionally the utility model provides a method of use has the universality, can be according to the structure change slide caliper rule outward appearance of waiting to examine the sample.

Drawings

FIG. 1 is a schematic structural view of a full caliper according to the present invention; 1 is a horizontal strip and a base; 2 is a vertical strip;

FIG. 2 is a schematic diagram of a kit structure for the full caliper measurement of the present invention; in the figures, 3-6 are grooves for filling reagents in the kit and detecting the filling amount by using full-inspection calipers respectively;

FIG. 3 is a schematic view of a method of using the full caliper according to the present invention; 7 is the kit to be detected after the reagent is filled; 8 is the full detection caliper; and 9 is an indication that the filling quantity is detected by a full check caliper.

Detailed Description

The invention will be further elucidated by means of embodiments with reference to the drawings.

In the case of the example 1, the following examples are given,

the structural schematic diagram of a full-check caliper is shown in fig. 1, and a horizontal strip (shown in fig. 1) with a certain width is used for ensuring the horizontality of the caliper and can also be provided with a base to increase the stability of the caliper; 2) several vertical strips (2 shown in fig. 1) perpendicular to the horizontal strips with a certain width are used for checking the reagent filling amount. It should be noted that, the height of the vertical strips of the present invention should be consistent with the theoretical liquid level height of the reagent to be detected after filling, and the number of the vertical strips should be consistent with the type of the reagent to be detected after filling; the distance between the vertical strips is consistent with the position of the reagent to be detected after filling.

The structure of one selected kit is shown in fig. 2. When the reagent box is produced, 4 grooves of the reagent box are filled with reagents of different types and different volumes simultaneously through a 4-channel filling machine. From left to right, the tanks 3-6 are filled with 120ml, 80ml, 70ml and 500ml of reagents in sequence. The vertical heights of the liquid levels (7 shown in fig. 3) in the tanks 3-6 after filling were measured to be 85mm, 80mm, 78mm, and 82mm, respectively. And (3) manufacturing a full-detection caliper (8 shown in figure 3), wherein the heights from the left to the right of the upper edges of the 4 vertical strips to the bottom end of the horizontal strip are respectively 85mm, 80mm, 78mm and 82 mm.

When the kit is used, the kit to be detected is placed on a horizontal table, the horizontal strip of the full-detection caliper is tightly attached to the table top, and the vertical strip is vertically upwards erected; then, horizontally pushing the caliper to enable the caliper to be attached to a kit to be tested (9 shown in figure 3), and ensuring that the position of the vertical strip is coincident with the position of the liquid level after filling to be tested; and turning on the laser light source, and respectively adjusting the positions of the 4 light sources to enable the positions to reach the same height as the liquid level positions of the 4 reagent bins. The laser light source penetrates through the vertical strip to form projection on the vertical strip, and if the projection position is the same as the scale on the vertical strip, the filling amount is qualified; if the liquid level is higher than the upper edge of the vertical bar of the caliper, the filling amount is over; if the liquid level is lower than the upper edge of the vertical bar of the caliper, the filling amount is not enough.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention should be covered by the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A gene sequencing kit detection system is characterized by comprising a kit full detection caliper, a light source and a gene sequencing kit; the full-detection caliper of the kit comprises a horizontal strip, a vertical strip and a handle; the vertical strip is provided with positioning scales; the horizontal strips have the function of stable planar placement and fixation; the vertical strip is used for comparing with the liquid level of the kit and judging the liquid loading amount of the kit; the up and down position of the light source can be moved and aligned with each of the separate reagent compartments in the reagent cartridge.

2. The gene sequencing kit detection system of claim 1, wherein the number of light sources is the same as the number of individually partitioned reagent compartments in the kit that need to be detected.

3. The gene sequencing kit detection system of claim 1, wherein the full caliper of the kit and the light source are fixed on the same base.

4. The gene sequencing kit detection system of claim 1, wherein the width of the vertical strip is 1-5 cm.

5. The gene sequencing kit detection system of claim 1, wherein the vertical strip is opaque.

6. The gene sequencing kit detection system of claim 1, further comprising a background plate; the vertical strips are transparent, the light source is a focusing light source, and the projection of the transparent vertical strips is projected onto the background plate.

7. The gene sequencing kit detection system of claim 1, wherein the kit has 4 separated reagent chambers, and the horizontal bar has 4 reagent chambers.

8. The gene sequencing kit detection system of claim 1, wherein the light source is a laser light source, and the spot size of the laser is 0.2-3 mm.