CN210058322U

CN210058322U - Auxiliary device for enzyme label plate

Info

Publication number: CN210058322U
Application number: CN201920697151.5U
Authority: CN
Inventors: 许世洁; 张俊涛; 闫桂敏
Original assignee: Heilongjiang Jinyu Medical Laboratory Ltd
Current assignee: Heilongjiang Jinyu medical laboratory Co., Ltd
Priority date: 2019-05-15
Filing date: 2019-05-15
Publication date: 2020-02-14
Anticipated expiration: 2029-05-15

Abstract

The embodiment of the utility model discloses an auxiliary device for ELIAS plate, include: the base comprises a bottom wall and two side walls, and the two side walls are oppositely arranged on the bottom wall; the positioning plate is arranged in the base, is opposite to the bottom wall and is arranged at intervals, an ELISA plate is placed between the positioning plate and the bottom wall, a through hole with a mark is formed in the positioning plate, and the through hole is used for corresponding to a reaction hole in the ELISA plate; and the positioning plate is positioned between the light screen and the bottom wall, and the light screen is arranged on the two side walls in a sliding manner so as to shield or expose the positioning plate. The utility model discloses the application has the locating plate and the gliding light screen of sign, judges whether accomplish the application of sample fast, accurately to avoid the pollution that conventional light-resistant mode brought.

Description

Auxiliary device for enzyme label plate

Technical Field

The utility model relates to an experimental device technical field especially relates to an auxiliary device for ELIAS plate.

Background

Enzyme-linked immunosorbent assays (ELISA) are usually carried out in the laboratory or in the laboratory sector, using enzyme-labeled plates, which are generally made of polystyrene. The microplate may be classified into 16 wells, 48 wells and 96 wells according to the number of reaction wells, and among them, a 96-well microplate is most common. The conventional ELISA experiment process usually includes the basic steps of sample adding, enzyme labeling antibody adding, substrate liquid adding and light shielding.

When carrying out above experimental procedure, because serum, diluent and enzyme-labeled antibody color are similar and mostly transparent liquid, when carrying out manual sample addition, especially under the great condition of sample size, the condition of aggravating, adding by mistake and adding the hourglass very easily appears in manual sample addition. Meanwhile, it is usually necessary to protect from light after the addition of the substrate solution is completed. The most common light-proof method in the laboratory at present is to stick adhesive paper at the reaction hole. However, during the operation, the situation that the liquid in the reaction hole is taken out is easily caused in the process of removing the gummed paper, so that the pollution close to the reaction hole is caused, and the accuracy of the experimental result is greatly influenced.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an auxiliary device for ELIAS plate aims at solving and is experimenting or the in-process of inspection using the ELIAS plate, is not convenient for carry out the application of sample accurately and because of the improper problem that causes the emergence of pollution of light-resistant.

In order to solve the above problem, the utility model provides an auxiliary device for ELIAS plate, include: the base comprises a bottom wall and two side walls, and the two side walls are oppositely arranged on the bottom wall; the positioning plate is arranged in the base, is opposite to the bottom wall and is arranged at intervals, an ELISA plate is placed between the positioning plate and the bottom wall, a through hole with a mark is formed in the positioning plate, and the through hole is used for corresponding to a reaction hole in the ELISA plate; and the positioning plate is positioned between the light shielding plate and the bottom wall, and the light shielding plate is arranged on the two side walls in a sliding manner so as to shield or expose the positioning plate.

In a preferred embodiment, each of the side walls is provided with a slide groove in which the light shielding plate is movable.

In a preferred embodiment, the light shielding plate includes a substrate and a light shielding layer covering the substrate.

In a preferred embodiment, the base is further provided with a second opening portion for allowing the microplate to enter between the positioning plate and the bottom wall.

In a preferred embodiment, the projection of the reaction hole on the bottom wall falls within the range of the projection of the through hole on the bottom wall.

In a preferred embodiment, the positioning plate is glued to the base or is integrally formed with the base.

In a preferred embodiment, the positioning plate is clamped on the base, or opposite fixing grooves are arranged on the base, and the positioning plate is placed in the fixing grooves and connected with the base.

In a preferred embodiment, the extension of the microplate in the direction from one side wall to the other side wall is equal to the distance between the two side walls, and the extension of the microplate in the direction perpendicular to the direction from one side wall to the other side wall is equal to the extension of the side walls in that direction.

In a preferred embodiment, the side of the shading plate remote from the bottom wall is provided with an identification area.

Implement the embodiment of the utility model provides a, will have following beneficial effect:

the auxiliary device is adopted, when the experiment is carried out, whether the sample adding is completed or not can be judged quickly and accurately through the marks corresponding to the reaction holes of the ELISA plates on the positioning plates, and meanwhile, after the sample adding is completed, the light shielding plates arranged on the two side walls can be moved to shield the ELISA plates located below the light shielding plates, so that the light shielding of the sample is completed conveniently and quickly, and the pollution of the sample caused by misoperation is avoided.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Wherein:

FIG. 1 is a schematic diagram of the composition structure of an embodiment;

FIG. 2 is a cross-sectional view of a visor of one embodiment;

FIG. 3 is a top view of a shutter plate of an embodiment.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the invention, which is defined by the appended claims.

As shown in fig. 1, for the schematic view of the composition structure of an embodiment of the present invention, referring to fig. 1, the auxiliary device for an elisa plate mainly comprises: a base 10, a positioning plate 20, and a light shielding plate 30. The base 10 comprises a bottom wall 11 and side walls 12, in the present embodiment, the base 10 is a rectangular parallelepiped structure, wherein two side walls 12 are provided, in other embodiments, the base 10 may adopt other different structures and shapes according to the shape of the matched elisa plate. The base 10 is provided with a slide groove, a first opening 13, and a second opening 14. The slide grooves are provided on opposite side walls 12 of the base 10, a first opening 13 is provided on the top of the base 10, and a second opening 14 is provided between the side walls 12 of the base 10. The base 10 includes at least one second opening 14, in this embodiment, two second openings 14 are provided on the side walls 12 opposite to the base 10, and the external dimensions of the second openings 14 are the same as those of the side walls 12. And in this case, the extension dimension of the microplate in the direction from one side wall 12 to the other side wall 12 is equal to the distance between the two side walls 12, and the extension dimension of the microplate in the direction perpendicular to the direction from one side wall 12 to the other side wall 12 is equal to the extension dimension of the side wall 12 in that direction, so that the entry of an external microplate into the base 10 is facilitated, and the position of the microplate in the base 10 is easily fixed.

As shown in fig. 1, the positioning plate 20 is disposed inside the base 10, and a gap exists between the positioning plate 20 and the bottom wall 11 of the base 10, so that when the microplate enters the base 10 from the second opening portion 14, the microplate can be positioned between the positioning plate 20 and the bottom wall 11 of the base 10. Preferably, the distance between the positioning plate 20 and the base 10 is the same as the thickness of the microplate, and thus fixation of the microplate can be achieved when the microplate enters the base 10 from the second opening portion 14. Locating plate 20 is through dismantling or fixed connection's mode installation, fixing on base 10, wherein can adopt the mode of splicing or integrated into one piece to fix locating plate 20 on base 10, also can adopt the joint simultaneously or set up relative fixed slot at the side, the locating plate is placed in the fixed slot and is connected with the base, realizes changing different locating plates 20 according to the type of the ELIAS plate of difference, improves the suitability of this device. The positioning plate 20 comprises through holes 21 with marks, wherein the number and the positions of the through holes 21 correspond to those of reaction holes of the microplate. In this embodiment, a 96-well plate is used as the microplate, and therefore, the positioning plate 20 is also provided with 96 through holes 21. In order to facilitate the user to identify whether the sample adding is finished or not, a digital mark is arranged above or below the through hole 21 and can be matched with the experiment number of the sample, and an experimenter can judge whether the sample adding is finished or not by comparing the digital mark with the experiment number. In order to facilitate the user to add a sample to the reaction well of the microplate and observe the condition of the sample in the reaction well, the diameter of the through-hole 21 is preferably larger than that of the reaction well of the microplate. In a preferred embodiment, the positioning plate 20 is made of a transparent material, which facilitates the user to observe the position of the microplate through the positioning plate 20.

As shown in fig. 1, 2 and 3, the light-shielding plate 30 is disposed above the positioning plate 20, and in the present embodiment, the sliding grooves of the base 10 are located above the positioning plate 20, and the light-shielding plate 30 is located in the opposite sliding grooves and can move in the sliding grooves to shield or expose the positioning plate 20 located below the light-shielding plate 30. In order to achieve the shielding of the light by the light shielding plate 30, it is preferable that the light shielding plate 30 is made of a material which does not transmit light. In other embodiments, the shading plate 30 includes a substrate 31 and a shading layer 32, and the shading layer 32 covers the substrate 31, wherein the shading layer 32 may be a black film which does not transmit light. Meanwhile, in order to prevent contamination and interference with experimental results, the light shielding plate 30 may be made of a plastic material that is easy to clean. Preferably, an identification area 33 may be provided on the visor 30, for example, numbers 1-12 may be printed on the identification area 33 on the visor 30 to facilitate the experimental operation.

In use, for example, in the three tests for gastritis, 96-well microplate is usually used, such as one using vertical plate arrangement, and numbers are indicated in the vertical arrangement sequence from 1 to 86 starting from the first column of 6-well on the positioning plate 20. When a horizontal 96-well microplate is used, the numbers are indicated in the order of the horizontal arrangement from 1 to 86 starting from the 4 th well in the first row. In conducting the experiment, the experimenter will first order the samples by experiment number according to conventional procedures, typically starting with 1. The ELISA plate is placed below the positioning plate 20 through the second opening part 14, so that the reaction holes of the ELISA plate correspond to the through holes 21 one by one, or the device and the ELISA plate are packaged together, and the ELISA plate is located at a required position before use. The shutter 30 is moved to expose the positioning plate 20 and microplate thereunder. Serum diluent is added into the reaction holes, then serum is added, wherein the serum with the label of 1 is added into the reaction holes corresponding to the through holes 21 with the label of 1, and then the step that experimenters need to count the labels of the holes in the traditional experiment is omitted. After the serum sample is added, the light shielding plate 30 is moved to shield the positioning plate 20 and the ELISA plate below, and the whole is placed in a water bath box. After the test is completed, the positioning plate 20 and the light shielding plate 30 are cleaned and can be used again.

Through this auxiliary device, the experimenter can compare with the reference numeral that awaits measuring on the sample through the locating plate 20 that has the reference numeral to help the experimenter to avoid adding by mistake, neglecting to add and repeated application of sample. Meanwhile, the design of the sliding light shielding plate 30 can avoid pollution caused by sticking gummed paper in the conventional process. In addition, this device repeatedly usable practices thrift the cost.

Claims

1. An assistance device for a microplate, comprising:

the base comprises a bottom wall and two side walls, and the two side walls are oppositely arranged on the bottom wall;

the positioning plate is arranged in the base, is opposite to the bottom wall and is arranged at intervals, an ELISA plate is placed between the positioning plate and the bottom wall, a through hole with a mark is formed in the positioning plate, and the through hole is used for corresponding to a reaction hole in the ELISA plate; and

the positioning plate is positioned between the light shielding plate and the bottom wall, and the light shielding plate is arranged on the two side walls in a sliding mode so as to shield or expose the positioning plate.

2. An assistance device for a microplate according to claim 1, wherein each of said side walls is provided with a slide groove in which said light-shielding plate is movable.

3. The assistance device for microplate according to claim 1, wherein the light shielding plate comprises a substrate and a light shielding layer, and the light shielding layer covers the substrate.

4. The assistance device for a microplate according to claim 1, wherein the base is further provided with a second opening portion for the microplate to enter between the positioning plate and the bottom wall.

5. The assistance device for a microplate according to claim 1, wherein a projection of the reaction well on the bottom wall falls within a projection range of the through-hole on the bottom wall.

6. The assistance device for a microplate according to claim 1, wherein the positioning plate is glued to the base or is integrally formed with the base.

7. The assistance device for microplate according to claim 1, wherein the positioning plate is clipped on the base, or opposite fixing grooves are provided on the base, and the positioning plate is placed in the fixing grooves and connected with the base.

8. The assistance device for a microplate according to claim 1, wherein the extension of said microplate in the direction from one of said side walls to the other of said side walls is equal to the distance between said side walls, and the extension of said microplate in the direction perpendicular to the direction from one of said side walls to the other of said side walls is equal to the extension of said side walls in that direction.

9. The assistance device for a microplate according to claim 8, wherein a side of the light-shielding plate away from the bottom wall is provided with a marking region.