CN214585497U

CN214585497U - Multichannel full-automatic dry type fluorescence immunoassay appearance

Info

Publication number: CN214585497U
Application number: CN202120806677.XU
Authority: CN
Inventors: 黄任强; 王沂
Original assignee: Shanghai Lehe Biotechnology Co ltd
Current assignee: Shanghai Lehe Biotechnology Co ltd
Priority date: 2021-04-20
Filing date: 2021-04-20
Publication date: 2021-11-02
Anticipated expiration: 2031-04-20

Abstract

The utility model discloses a full-automatic dry fluorescence immunoassay appearance of multichannel, the setting hangs the sampling needle in draw-in groove subassembly top, under the drive of horizontal/perpendicular lead screw motor, the sampling needle falls into sample reagent pipe, behind the plunger pump drive suction sample liquid, perpendicular lead screw motor promotes the sampling needle and leaves sample reagent pipe, horizontal lead screw motor drive sampling needle removes to detect reagent card top, inject sample liquid into the plunger pump drive sampling needle to detecting reagent card, then the detecting component carries out the fluorescence detection to detecting reagent card, obtain fluorescence intensity's the signal of telecommunication. And the sampling needle moves into the needle washing groove to be washed and then moves into the second sample reagent tube to extract new sample liquid. Through setting up the needle washing groove, wash the sampling needle after a sampling, the utility model discloses guaranteed that cross contamination can not take place between each sample liquid to can satisfy the requirement that detects and many projects detect in batches, can carry out whole batch's detection, the detection personnel's that significantly reduces intensity of labour automatically.

Description

Multichannel full-automatic dry type fluorescence immunoassay appearance

Technical Field

The utility model belongs to the technical field of fluorescence immunoassay, concretely relates to full-automatic dry type fluorescence immunoassay appearance of multichannel.

Background

Fluorescence Immunoassay (FIA) is an immunoassay technique in which an antibody or an antigen is labeled with a fluorescent substance as a tracer, and combines the specificity of the reaction between the antigen and the antibody in immunology and the sensitivity of the fluorescence technique. The principle is similar to ELISA, fluorescent substance is used as a labeling probe, the fluorescent substance is combined with known antibody or antigen to form a fluorescent antibody compound or a fluorescent antigen compound, then the fluorescent compound is used as a capture reagent and is fixed on a carrier such as a glass cellulose film or a polyester film, a nitrocellulose film which is matched with the fluorescent antibody or antigen is embedded at a specific position is used as a detection reagent, the two kinds of fluorescent compounds are mutually connected to be used as a stationary phase, an analyte is used as a mobile phase, the analyte is moved on a film strip for a period of time through capillary action, and then an immunofluorescence compound with antigen and antibody specific reaction is formed at the specific position of the nitrocellulose film, and the fluorescence intensity of the fluorescent substance is detected. The fluorescence intensity is in negative correlation with the analyte concentration in the immune competition method, and in positive correlation with the analyte concentration in the double-antibody sandwich method. By measuring the fluorescence intensity at this location, a mathematical model is constructed and the concentration of the analyte in the analyte can be calculated. The fluorescence immunoassay analyzer is designed based on an immunofluorescence technique and combined with a chromatography technique and a laser induction technique. After the immune reaction is finished, when the immune reaction is measured, a semiconductor laser emits laser with a specific wavelength to irradiate a sample, fluorescence with a certain wavelength is excited, the fluorescence is received by a photosensitive diode, and an optical signal is converted into a voltage signal corresponding to light intensity. The concentration and amount of the analyte can then be measured based on the linear relationship between the intensity of the fluorescent light and the concentration value of the analyte.

However, in order to prevent cross contamination, the fluorescence analyzer of the prior art can only put one sample, usually one detection reagent card, at a time, and after the detection is completed, the detection reagent card with completed detection is taken out, and put in a new detection reagent card, and then the detection is repeated. Therefore, in the detection process, the manual operation cannot be carried out, and the mechanical manual operation is adopted, so that the labor intensity of detection personnel is seriously increased, the labor efficiency is reduced, and manpower and material resources are wasted.

Thus, the prior art is subject to further improvements and enhancements.

Disclosure of Invention

In view of the above-mentioned defect of prior art, the utility model aims to provide a full-automatic dry fluorescence immunoassay appearance of multichannel to solve prior art's fluorescence immunoassay appearance, in order to avoid cross contamination, once can only put into a detect reagent card, whole technological defect that needs artifical the participation.

The utility model discloses a multi-channel full-automatic dry type fluorescence immunoassay analyzer, which comprises a clamping groove component, a sample adding component and a detection component; the clamping groove assembly comprises a test tube slot for placing at least one sample reagent tube, a clamping groove for inserting at least one detection reagent card and a card falling support opposite to each clamping groove; the clamping groove is connected with a card dropping motor through a transmission belt, and can move relative to the card dropping bracket under the driving of the card dropping motor; the sample adding assembly comprises a hollow sampling needle which is hung above the clamping groove assembly and has a downward needle head, and the needle tail of the sampling needle is connected with a plunger pump for sucking and pushing out sample liquid; the sampling needle is connected with at least one horizontal screw motor and can move between the sample reagent tube and the detection reagent card under the drive of the horizontal screw motor; the sampling needle is also connected with a vertical screw motor and can be lifted under the driving of the vertical screw motor; the detection assembly comprises a light source for emitting fluorescence and a fluorescence plate for receiving the fluorescence and detecting the fluorescence intensity, and is used for carrying out fluorescence detection on the detection reagent card, and the fluorescence plate converts a light intensity signal of the fluorescence into an electric signal; the liquid path system comprises a liquid path pipeline, and the liquid path pipeline is respectively communicated with a clear liquid tank for storing clean cleaning liquid, a waste liquid tank for storing cleaned waste liquid and a needle washing groove for cleaning the sampling needle.

Preferably, the liquid path system further includes a diaphragm pump for driving the flow of the cleaning liquid inside the liquid path pipe, the diaphragm pump driving the cleaning liquid, flowing from the clear liquid tank into the needle wash tank, and flowing from the needle wash tank into the waste liquid tank.

More preferably, the diaphragm pumps are arranged in two, one drives the cleaning liquid to pass through the sampling needle from the clear liquid tank and be injected into the needle washing groove; and the other driving cleaning liquid is sucked out from the upper part of the needle washing groove and flows into the waste liquid tank.

Preferably, one end of the card slot is provided with a positioning switch for determining an origin position of the card slot.

Preferably, a test tube detection switch for detecting whether a sample reagent tube is inserted is disposed at a side surface of the test tube insertion slot.

Preferably, a reagent card detection switch for detecting whether a detection reagent card is inserted is arranged on a side surface of the card slot.

More preferably, the cuvette detection switch and/or the reagent card detection switch is a photosensor.

Preferably, the side surface of the test tube slot is provided with a code scanning assembly, the code scanning assembly is used for reading and identifying a bar code on the side surface of each sample reagent tube, and the bar code records sample information in the sample reagent tube.

More preferably, the code scanning component is an optoelectronic identification component.

Preferably, a computer for acquiring said electrical signals is also connected/integrated.

Preferably, a thermal printer for printing the detection result is also connected/integrated.

Preferably, the card dropping device further comprises a waste card drawer for collecting the detection reagent cards pushed down by the card dropping bracket.

More preferably, the waste card drawer is further provided with a waste card capacity identification component for detecting the stacking height of the waste cards in the waste card drawer.

Further preferably, the waste card capacity identification module includes an ultrasonic detection module for detecting a stacking height of the waste cards by ultrasonic waves.

Further preferably, the card waste capacity identification component further comprises a prompt module.

Still further preferably, the prompting module comprises a voice prompting module and/or a light prompting module.

The utility model discloses a working method of full-automatic dry fluorescence immunoassay appearance of multichannel, wherein, including the step:

a. the horizontal screw motor drives the sampling needle to move to the upper part of the sample reagent tube;

b. the vertical screw motor drives the sampling needle to descend to the sample reagent tube;

c. the plunger pump drives the sampling needle to suck sample liquid from the sample reagent tube;

d. the vertical screw motor drives the sampling needle to ascend and leave the sample reagent tube;

e. the horizontal screw motor drives the sampling needle to move to the position above the detection reagent card;

f. the plunger pump drives the sampling needle to inject sample liquid into the detection reagent card;

g. the horizontal screw motor drives the sampling needle to move to the position above the needle washing groove;

h. and the vertical screw rod motor drives the sampling needle to descend into the needle washing groove.

Preferably, the method further comprises the steps of:

i. the vertical screw motor drives the sampling needle to move up and down in the needle washing groove at least one way;

j. the plunger pump sucks and pushes the cleaning liquid to and fro at least one time;

i and j are not in sequence.

Has the advantages that: the utility model discloses a full-automatic dry fluorescence immunoassay appearance of multichannel hangs in draw-in groove subassembly top, syringe needle downwards, hollow sampling needle through the setting, under the drive of horizontal lead screw motor and perpendicular lead screw motor, the sampling needle falls into sample reagent pipe, behind the plunger pump drive suction sample liquid, perpendicular lead screw motor promotes the sampling needle and leaves sample reagent pipe, and horizontal lead screw motor drive sampling needle removes to detect reagent card top, plunger pump drive sampling needle to inject sample liquid in the detect reagent card, then the detecting component carries out the fluorescence detection to the detect reagent card, obtains fluorescence intensity's the signal of telecommunication, and the detect reagent card of having accomplished the detection then leaves by falling the card support and pushing away the draw-in groove. And the sampling needle moves into the needle washing groove to be washed, then moves into a second sample reagent tube to extract new sample liquid, and the process is repeated. The utility model discloses a full-automatic dry fluorescence immunoassay appearance of multichannel washs the sampling needle through setting up the needle washing groove after a sampling, has guaranteed that cross contamination can not take place between each sample liquid to can satisfy the requirement that detects and multinomial detection in batches, but the whole batch of detection of automatic execution, the intensity of labour who has significantly reduced testing personnel.

The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings, so as to fully understand the objects, the features and the effects of the present invention.

Drawings

Fig. 1 is an external overall structure diagram of the multi-channel full-automatic dry type fluorescence immunoassay analyzer of the utility model.

Fig. 2 is the internal structure schematic diagram of the first angle of the multi-channel full-automatic dry type fluorescence immunoassay analyzer of the present invention.

Fig. 3 is a schematic view of the structure of the groove assembly of the multi-channel full-automatic dry-type fluorescence immunoassay analyzer of the present invention.

Fig. 4 is the waste card capacity identification component structure diagram of the multi-channel full-automatic dry type fluorescence immunoassay analyzer of the utility model.

Fig. 5 is the internal structure diagram of the second angle of the multi-channel full-automatic dry type fluorescence immunoassay analyzer of the present invention.

Fig. 6 is a schematic diagram of the structure of the liquid path system of the multi-channel full-automatic dry-type fluorescence immunoassay analyzer of the present invention.

FIG. 7 is a sectional view showing the structure of the needle washing tank of the multi-channel full-automatic dry fluoroimmunoassay analyzer of the present invention.

In the figure, 1-machine box door, 2-waste card drawer, 3-data interface, 4-test tube slot, 5-card slot card inserting port, 6-computer, 7-thermal printer, 8-power button, 9-bottom plate, 10-sample reagent tube, 11-card slot component bracket, 12-card table, 13-positioning switch, 14-card dropping port, 15-card dropping bracket, 16-card dropping driving belt, 17-waste card capacity identification component, 18-card dropping motor, 19-card slot guide rail, 20-sampling needle, 21-sample adding control plate, 22-transverse screw rod motor, 23-longitudinal screw rod motor, 24-vertical screw rod motor, 25-code scanning component, 26-control plate driving belt, 27-sample adding guide rail, 28-flexible pipe, 30-mounting bracket, 31-ultrasonic detection module, 32-plunger pump, 33-liquid pipeline, 34-clear liquid tank, 35-waste liquid tank, 36-needle washing tank, 37-diaphragm pump and 38-deep hole; 39-waste liquid hole; 40-waste liquid outlet.

Detailed Description

The utility model provides a multichannel is full-automatic dry type fluorescence immunoassay appearance, for making the utility model discloses a purpose, technical scheme and effect are clearer, clear and definite, and it is right with the example that reference the figure below makes further detailed description the utility model discloses. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The utility model provides a multichannel full-automatic dry type fluorescence immunoassay appearance, a preferred embodiment, as shown in figure 1, from the outside, the shell that is a hexahedron structure basically encloses. For convenience of operation, the human-computer interaction part is basically arranged on the front and the side, and may be arranged on other parts, which are not limited herein. In the preferred embodiment, the front face of the hexahedron, i.e. the face facing the operator, comprises two parts, wherein the lower part of the front face is provided with a test tube slot 4, and at least one sample reagent tube 10 containing a sample to be tested is placed upright in said test tube slot 4 in turn. In a preferred embodiment, a test tube detection switch, such as a photoelectric sensor, is disposed on a side surface of the test tube slot 4 for detecting whether the sample reagent tube 10 is inserted.

And a clamping groove inserting opening 5 is arranged beside the test tube inserting groove 4 and communicated with a clamping groove in the shell. The clamping groove comprises a clamping groove frame and at least one clamping table 12 which is arranged along the side face of the clamping groove frame in a scattered mode at equal intervals. Empty detect reagent card, from draw-in groove plug-in card mouth 5 handle go into card platform 12 on, put into regular array, make things convenient for follow-up automatic application of sample and detection operation. Correspondingly, a reagent card detection switch, such as a photoelectric sensor, for detecting whether a detection reagent card is inserted is also arranged on the side surface of the card slot.

And a computer 6 is arranged at the upper part of the front, and a data processing system is arranged on the computer 6 and is used for processing the data acquired by the fluorescence detection. Meanwhile, the computer 6 can also be set as a man-machine interaction control center, and only corresponding software and hardware are required to be installed, which is a common technology in the field of numerical control and is not described in detail herein. In view of the reduced size and convenient installation, the computer 6 is preferably a liquid crystal display, especially a liquid crystal display of a touch screen, so as to facilitate human-computer interaction. For example, a palm computer is embedded in the upper part of the front face, and is preferably slightly inclined upwards, so that the reading and the operation are convenient.

In a more preferred embodiment, a printer is further provided at the upper front, near the computer 6, and in view of its small size, a thermal printer 7 is preferably used to print fluorescence detection data in real time.

Preferably, considering that the multi-channel full-automatic dry-type fluorescence immunoassay analyzer of the present application may be a common instrument and a plurality of test items may be performed simultaneously, in a preferred embodiment, the side surface is further provided with a data interface 3, which may be a plurality of types of data interfaces, such as a USB interface, a network cable interface, and the like, so as to facilitate data exchange with the computer 6. When the system is used, information of each test item is stored in an item ID card, such as an IC card, or other flash memory cards or U disks, and when a batch of samples of a certain item are detected, the corresponding item ID card is inserted into the ID card slot, and the test item to be executed is automatically identified by the system and is guided into the computer 6. And corresponding results obtained by subsequent tests are also automatically stored in the test items, so that the sample information detected by each test item can be separately stored without confusion.

In view of the ease of operation, the power button 8 for switching on and off is also preferably provided on the side or front, for example, next to the data interface 3 in fig. 1.

In the inside of shell, then be equipped with each corresponding operating device and circuit structure, in order to accomplish the utility model discloses a full-automatic fluorescence detection function of multichannel. In one embodiment, as shown in fig. 2, a card slot assembly, a sample adding assembly, and a detection assembly are disposed inside the housing.

The card slot assembly is shown in fig. 3 and comprises a card slot for inserting a detection reagent card and a card falling bracket 15 facing each card slot; the card dropping motor 18 is connected with and drives all the card slots to move horizontally through the card dropping transmission belt 16. In operation, all the card slots will move, preferably step-by-step, along the length direction of the card slot guide rail 19 towards the card dropping bracket 15 under the driving of the card dropping motor 18, i.e. the card dropping motor 18 is preferably a stepping motor. The card falling bracket 15, as shown in fig. 3, includes a row of parallel flat rods facing each card slot and approximately horizontally disposed, and is fixed to the card slot assembly bracket 11 at a certain height, i.e., a height lower than the height of the detection reagent card, by means of, for example, a vertical rod. Therefore, when the card slot moves to the card falling bracket 15 along with the card falling driving belt 16, each flat rod is inserted into the card slot opposite to the flat rod in parallel, and pushes down the first detection reagent card encountered and leaves the card slot. The first detection reagent card is the detection reagent card which just completes the fluorescent irradiation and detection. Therefore, only after the detection is finished, the card dropping motor 18 drives the card slot to move one grid step by step towards the card dropping support 15, and the first detection reagent card at the moment is pushed by the card dropping support 15 to fall into the lower part of the card slot assembly, for example, the card slot assembly support 11 to wait for cleaning and recycling.

In consideration of the requirement of accurate positioning, one end of the card slot is provided with a positioning switch, such as an optoelectronic switch or a magnetic induction switch, for determining the original point position of the card slot.

Preferably, a bayonet 14 is respectively arranged on the slot module bracket 11 right below the front part of the tip of each flat rod. The first detection reagent card falls down after being pushed out, and penetrates out of the card slot component support 11 from the card slot 14, so that collection is more convenient.

In a better embodiment, a drawable waste card drawer 2 is further arranged right below the card dropping opening 14, and a detection reagent card completing fluorescence detection is pushed by the card dropping bracket 15 from the card table 12 through the card dropping opening 14 to fall into the waste card drawer 2. Meanwhile, a cabinet door 2 is arranged on the side face of the shell, and the cabinet door can be opened to clean waste cards accumulated in the shell, particularly pull out the waste card drawer 2 to clean the waste cards in the shell.

More preferably, the waste card drawer 2 is further provided with a waste card capacity recognition module 17 on a side surface, for example, an upper side surface, for detecting a stacking height of the waste cards in the waste card drawer 2. In a preferred embodiment, the specific structure of the waste card capacity identification module 17 is shown in fig. 4, and includes a mounting bracket 30 erected on the side of the waste card drawer 2, and an ultrasonic detection module 31 is installed at a certain height of the mounting bracket 30, and detects the stacking height of the waste cards in the waste card drawer 2 by ultrasonic waves. In a preferred embodiment, the waste card capacity identification module 17 further comprises a prompt module for giving a prompt by means of sound, light, etc. to remind the operator to pull out the waste card drawer 2 and clean the accumulated waste cards. The ultrasonic detection module 31 and/or the prompt module are/is the prior art, and are not described herein again.

The sample feeding assembly comprises a hollow sampling needle 20 suspended above the card slot assembly with the needle facing downward, and a plunger pump 32 connected to the needle end of the sampling needle 20, preferably via a flexible tube 28, for sucking and pushing out sample liquid. The sampling needle 20 is connected to at least one horizontal screw motor, typically two motors disposed orthogonally to each other for driving the sampling needle 20 to move in a horizontal direction, i.e., a transverse screw motor 22 and a longitudinal screw motor 23, so that the sampling needle 20 can move between the sample reagent tube 10 and the detection reagent card under the driving of the horizontal screw motor.

In a preferred embodiment, as shown in fig. 5, the sampling needle 20 is disposed at one end of a sample-adding control plate 21 and can be driven by a longitudinal screw motor 23 to move horizontally along the sample-adding control plate 21 in the longitudinal direction. The sample adding control plate 21 can be driven by the transverse screw rod motor 22 through the control plate transmission belt 26 to horizontally move along the sample adding guide rail 27. And, the sampling needle 20 is further connected with a vertical screw motor 24, when the sampling needle 20 moves horizontally to a position right above the sample reagent tube 10, the vertical screw motor 24 drives the sampling needle 20 to descend until the needle tip is inserted below a certain height of the liquid level in the sample reagent tube 10, and then the plunger pump 32 works to suck the sample liquid to be detected. After the suction is finished, the vertical screw motor 24 works, the sampling needle 20 is lifted, and then the sampling needle horizontally moves to a position right above the detection reagent card closest to the card falling bracket 15 in the card slot under the driving of the transverse screw motor 22 and the longitudinal screw motor 23.

Specifically, each detection reagent card is provided with a sample hole with a set diameter on the top surface, and a liquid detection sample can be injected into each detection reagent card from the sample hole and then subjected to subsequent fluorescence detection.

At this time, the sampling needle 20 may be driven by the vertical screw motor 24 to descend to be inserted into the sample adding hole, and then driven by the plunger pump 32 to push out the sample solution to be detected and inject the sample solution into the detection reagent card. Of course, as long as the position control is accurate, the sampling needle 20 may not be lowered into the sample addition hole, but the sample solution to be detected may be pushed out at a certain height and fall into the detection reagent card.

When considering to detect many samples multichannel, sampling needle 20 will insert into a plurality of sample reagent pipes 10, draws sample liquid many times, and this centre probably leads to cross contamination, so the utility model discloses a full-automatic dry fluorescence immunoassay appearance of multichannel still includes a liquid road system, as shown in fig. 6, liquid road system includes liquid road pipeline 33, communicates respectively and is used for depositing the clear solution jar 34 of clean washing liquid, is used for depositing the waste liquid jar 35 of waste liquid and is used for wasing the needle washing groove 36 of sampling needle 20. Thus, after the sampling needle 20 finishes sampling a sample reagent tube 10 and injecting the sample reagent tube into a corresponding detection reagent card, the sample reagent tube moves into the needle washing groove 36, is driven by the vertical screw rod motor 24 to wash the external old liquid up and down for several times, and is driven by the plunger pump 32 to suck and push out the washing liquid for several times to wash the interior of the sampling needle. After the cleaning is completed, the sampling needle 20 is driven to move to the next sample reagent tube 10 to continue sampling.

In view of the fact that the cleaning liquid in the needle cleaning tank 36 will be contaminated after several times of cleaning, in a preferred embodiment, the liquid path system further comprises a diaphragm pump 37 for driving the flow of the cleaning liquid inside the liquid path pipe 33 from the cleaning liquid tank 34 to the needle cleaning tank 36 and from the needle cleaning tank 36 to the waste liquid tank 35 after the cleaning is completed.

In a more preferred embodiment, the two diaphragm pumps 37 are provided, and the first diaphragm pump drives the cleaning liquid from the cleaning liquid tank 34, through the sampling needle 20, and into the needle washing groove 36; this cleans the lumen of the sampling needle 20. And the cleaning liquid is accumulated in the needle washing groove 36 until the cleaning liquid overflows the outside of the sampling needle 20, and at this time, the second diaphragm pump drives the cleaning liquid to be sucked out from the upper part of the needle washing groove 36 and flow into the waste liquid tank 35, so that the cleaning of the outside of the sampling needle 20 is completed. During the washing process, the washing liquid is preferably kept in the above flowing state, i.e. both said membrane pumps 37 are continuously operated to ensure the cleaning effect.

In a more preferred embodiment, as shown in the cross-sectional view of fig. 7, the bottom of the needle washing groove 36 is provided with a deep hole 38 for inserting the sampling needle 20, the cleaning liquid is driven by a first diaphragm pump and injected into the deep hole 38 from the needle head of the sampling needle 20, and at the same time, the other side of the bottom is provided with a waste liquid hole 39, the waste liquid hole 39 is connected with a waste liquid outlet 40, the waste liquid outlet 40 is connected with a second diaphragm pump, and the second diaphragm pump sucks the cleaning liquid out of the needle washing groove 36 and discharges the cleaning liquid into the waste liquid tank 35 through the liquid path pipeline 33. The flow direction of the cleaning liquid in the needle washing groove 36 is shown by the arrow in fig. 7.

And the detection reagent card after sample adding receives the fluorescence detection of the detection component. Specifically, the detection assembly comprises a light source emitting fluorescence and a fluorescent plate receiving the fluorescence and detecting the intensity of the fluorescence, and the fluorescent plate converts the light intensity signal of the fluorescence into an electric signal. Fluorescence detection is also a common technique in the art and will not be described in detail here.

In view of the fact that a plurality of sample reagent vessels 10 are to be placed at a time, for easy identification at the time of testing, each sample reagent vessel 10 is attached with a code label corresponding to the sample to be tested inside, and accordingly, a code scanning member 25, such as an optoelectronic identification member, is provided at the side of the test tube slot 4, so that each sample reagent vessel 10 inserted into the test tube slot 4, in which the number information, and/or the sample information, is automatically read and inputted into the computer 6, and preferably stored together with the test results obtained later.

The utility model discloses a working method of full-automatic dry fluorescence immunoassay appearance of multichannel summarizes as follows, including the step:

a. the horizontal screw motor drives the sampling needle 20 to move to the upper part of the sample reagent tube 10;

b. the vertical screw motor 24 drives the sampling needle 20 to descend to the sample reagent tube 10;

c. the plunger pump 32 drives the sampling needle 20 to suck the sample liquid from the sample reagent tube 10;

d. the vertical screw motor 24 drives the sampling needle 20 to ascend away from the sample reagent tube 10;

e. the horizontal screw motor drives the sampling needle 20 to move to the position above the detection reagent card;

f. the plunger pump 32 drives the sampling needle 20 to inject sample liquid into the detection reagent card;

g. the horizontal screw motor drives the sampling needle 20 to move above the needle washing groove 36;

h. the vertical screw motor 24 drives the sampling needle 20 to descend into the needle washing groove 36.

In a more preferred embodiment, the steps further comprise:

i. the vertical screw motor 24 drives the sampling needle 20 to move up and down in the needle washing groove 36 at least one round;

j. the plunger pump 32 at least one of sucks and pushes the cleaning liquid back and forth;

i and j are not in sequence.

To sum up, the utility model discloses a full-automatic dry fluorescence immunoassay appearance of multichannel hangs in draw-in groove subassembly top, syringe needle downwards, hollow sampling needle 20 through setting up, under horizontal lead screw motor and perpendicular lead screw motor 24's drive, sampling needle 20 falls into sample reagent pipe 10, behind the plunger pump 32 drive suction sample liquid, perpendicular lead screw motor 24 promotes sampling needle 20 and leaves sample reagent pipe 10, and horizontal lead screw motor drive sampling needle 20 removes to detect reagent card top, plunger pump 32 drive sampling needle 20 to inject sample liquid in the detect reagent card, then detecting component carries out the fluorescence detection to detect reagent card, obtains fluorescence intensity's the signal of telecommunication, and the detect reagent card of having accomplished the detection then is pushed down and leaves by falling card support 15 the draw-in groove. The sampling needle 20 moves into the needle washing groove 36 for washing, and then moves into the second sample reagent tube 10 for extracting a new sample liquid, and the process is repeated. The utility model discloses a full-automatic dry fluorescence immunoassay appearance of multichannel washs the needle groove 36 through setting up, washs the sampling needle after a sampling, has guaranteed that cross contamination can not take place between each sample liquid to can satisfy the requirement that detects and multinomial detection in batches, can carry out whole batch's detection automatically, the intensity of labour who has significantly reduced testing personnel.

The foregoing has described in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be devised by those skilled in the art in light of the teachings of the present invention without undue experimentation. Therefore, the technical solutions that can be obtained by a person skilled in the art through logic analysis, reasoning or limited experiments based on the prior art according to the concepts of the present invention should be within the scope of protection defined by the claims.

Claims

1. A multi-channel full-automatic dry type fluorescence immunoassay analyzer is characterized by comprising a clamping groove component, a sample adding component and a detection component;

the clamping groove assembly comprises a test tube slot for placing at least one sample reagent tube, a clamping groove for inserting at least one detection reagent card and a card falling support opposite to each clamping groove;

the clamping groove is connected with a card dropping motor through a transmission belt, and can move relative to the card dropping bracket under the driving of the card dropping motor;

the sample adding assembly comprises a hollow sampling needle which is hung above the clamping groove assembly and has a downward needle head, and the needle tail of the sampling needle is connected with a plunger pump for sucking and pushing out sample liquid;

the sampling needle is connected with at least one horizontal screw motor and can move between the sample reagent tube and the detection reagent card under the drive of the horizontal screw motor;

the sampling needle is also connected with a vertical screw motor and can be lifted under the driving of the vertical screw motor;

the detection assembly comprises a light source for emitting fluorescence and a fluorescence plate for receiving the fluorescence and detecting the fluorescence intensity, and is used for carrying out fluorescence detection on the detection reagent card, and the fluorescence plate converts a light intensity signal of the fluorescence into an electric signal;

the liquid path system comprises a liquid path pipeline, and the liquid path pipeline is respectively communicated with a clear liquid tank for storing clean cleaning liquid, a waste liquid tank for storing cleaned waste liquid and a needle washing groove for cleaning the sampling needle.

2. The multi-channel full automatic dry fluoroimmunoassay analyzer according to claim 1, wherein the liquid path system further comprises a diaphragm pump for driving the flow of the cleaning liquid inside the liquid path pipe, the diaphragm pump driving the cleaning liquid, flowing from the clear liquid tank into the needle washing tank, and flowing from the needle washing tank into the waste liquid tank.

3. The multi-channel full-automatic dry type fluorescence immunoassay analyzer according to claim 1 or 2, wherein one end of the card slot is provided with a positioning switch for determining the origin position of the card slot.

4. The multi-channel full-automatic dry type fluorescence immunoassay analyzer according to claim 1, wherein a reagent card detection switch for detecting whether a detection reagent card is inserted is arranged on the side surface of the card slot.

5. The multi-channel full-automatic dry type fluoroimmunoassay analyzer according to claim 1, wherein the test tube slot is provided with a barcode scanning assembly at the side for reading and identifying a barcode at the side of each sample reagent tube, wherein the barcode records the sample information in the sample reagent tube.

6. The multi-channel full automatic dry fluoroimmunoassay analyzer according to claim 1, further connected/integrated with a computer for collecting the electrical signals.

7. The multi-channel full-automatic dry type fluorescence immunoassay instrument according to claim 1, further comprising a waste card drawer for collecting the detection reagent card pushed down by the card dropping rack.

8. The multi-channel full-automatic dry type fluorescence immunoassay analyzer according to claim 7, wherein the waste card drawer is further provided with a waste card capacity identification component for detecting the stacking height of the waste cards in the waste card drawer.

9. The multi-channel full-automatic dry type fluoroimmunoassay analyzer according to claim 8, wherein the waste card capacity identification module comprises an ultrasonic detection module for detecting the stacking height of the waste cards by ultrasonic waves.