CN214585496U - Sampling needle cleaning structure for multi-channel dry-type fluorescence immunoassay analyzer - Google Patents
Sampling needle cleaning structure for multi-channel dry-type fluorescence immunoassay analyzer Download PDFInfo
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- CN214585496U CN214585496U CN202120806663.8U CN202120806663U CN214585496U CN 214585496 U CN214585496 U CN 214585496U CN 202120806663 U CN202120806663 U CN 202120806663U CN 214585496 U CN214585496 U CN 214585496U
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Abstract
The utility model discloses a sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure, sets up the liquid way system that the loading has the washing liquid, including the needle washing groove that is equipped with the washing liquid, the sampling needle accomplishes the sampling to the first sample liquid of the examination of awaiting measuring to pour into first testing reagent card back into, before removing the sample reagent pipe to the second sample liquid that is equipped with the examination of awaiting measuring, by horizontal lead screw motor and perpendicular lead screw motor drive, remove earlier and fall into the needle washing groove, wash the sampling needle by the washing liquid of washing in the needle groove. After the cleaning is finished, the sampling needle moves to the second sample reagent tube again, new sample liquid is extracted, and the process is circulated. Through setting up the needle washing groove, wash the sampling needle after once sampling, both guaranteed not to take place cross contamination between the different sample liquid, satisfy the requirement that detects in batches and multinomial detection, but the whole batch of detection of automatic execution again has significantly reduced measurement personnel's intensity of labour.
Description
Technical Field
The utility model belongs to the technical field of fluorescence immunoassay, concretely relates to sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure.
Background
Fluorescence Immunoassay (FIA) is an immunoassay technique in which an antibody or an antigen is labeled with a fluorescent substance as a tracer, and combines the specificity of the reaction between the antigen and the antibody in immunology and the sensitivity of the fluorescence technique. The principle is similar to ELISA, fluorescent substance is used as a labeling probe, the fluorescent substance is combined with known antibody or antigen to form a fluorescent antibody compound or a fluorescent antigen compound, then the fluorescent compound is used as a capture reagent and is fixed on a carrier such as a glass cellulose film or a polyester film, a nitrocellulose film which is matched with the fluorescent antibody or antigen is embedded at a specific position is used as a detection reagent, the two kinds of fluorescent compounds are mutually connected to be used as a stationary phase, an analyte is used as a mobile phase, the analyte is moved on a film strip for a period of time through capillary action, and then an immunofluorescence compound with antigen and antibody specific reaction is formed at the specific position of the nitrocellulose film, and the fluorescence intensity of the fluorescent substance is detected. The fluorescence intensity is in negative correlation with the analyte concentration in the immune competition method, and in positive correlation with the analyte concentration in the double-antibody sandwich method. By measuring the fluorescence intensity at this location, a mathematical model is constructed and the concentration of the analyte in the analyte can be calculated. The fluorescence immunoassay analyzer is designed based on an immunofluorescence technique and combined with a chromatography technique and a laser induction technique. After the immune reaction is finished, when the immune reaction is measured, a semiconductor laser emits laser with a specific wavelength to irradiate a sample, fluorescence with a certain wavelength is excited, the fluorescence is received by a photosensitive diode, and an optical signal is converted into a voltage signal corresponding to light intensity. The concentration and amount of the analyte can then be measured based on the linear relationship between the intensity of the fluorescent light and the concentration value of the analyte.
In order to prevent cross contamination, a fluorescence analyzer of the prior art can only put one sample, usually one detection reagent card, at a time, and after the detection is completed, an operator takes out the detection reagent card which completes the detection, puts in a new detection reagent card, and then repeatedly performs the detection. Therefore, in the detection process, the manual operation cannot be carried out, and the mechanical manual operation is adopted, so that the labor intensity of detection personnel is increased, the labor efficiency is reduced, and manpower and material resources are wasted.
There is a multi-channel fluorescence analyzer that can automatically process multiple samples. In order to prevent contamination between samples, the method is to switch the pipette head of a sampling needle for each sample to be tested. This method is not economical and poses a certain sealing risk, since frequent replacement of the pipette head is required.
Thus, the prior art is subject to further improvements and enhancements.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned defect of prior art, the utility model aims to provide a sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure to solve prior art's multichannel fluorescence immunoassay appearance, in order to avoid cross contamination, once can only put into a detect reagent card, or the switching needs the automatic technical defect who changes the straw bandeau and come the leakproofness problem when waiting to detect the sample.
The utility model discloses a sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure, wherein, multichannel dry-type fluorescence immunoassay appearance, include: the device comprises a test tube slot for placing a sample reagent tube and a clamping slot for placing a detection reagent card, wherein the orifice of the sample reagent tube is upwards placed on the test tube slot, and the top of the detection reagent card is provided with an opening with a set diameter; the sampling device also comprises a hollow sampling needle, wherein the needle head of the sampling needle is downward and movably suspended above the test tube slot and the clamping slot; the needle tail of the sampling needle is connected with a plunger pump for sucking and pushing out the sample liquid in the sampling needle; the sampling needle is connected with at least one horizontal screw motor and can horizontally move under the driving of the horizontal screw motor; the sampling needle is also connected with a vertical screw motor and can be lifted under the driving of the vertical screw motor; the device also comprises a liquid path system loaded with cleaning liquid, which comprises a liquid path pipeline, a liquid cleaning tank used for storing clean cleaning liquid, a waste liquid tank used for storing cleaned waste liquid and a needle cleaning tank filled with cleaning liquid, wherein the cleaning liquid is used for cleaning the sampling needle.
Preferably, the liquid path system further includes a diaphragm pump for driving the flow of the cleaning liquid inside the liquid path pipe, the diaphragm pump driving the cleaning liquid, flowing from the clear liquid tank into the needle washing tank, and flowing from the needle washing tank into the waste liquid tank.
More preferably, the diaphragm pump comprises two, a first diaphragm pump and a second diaphragm pump, the first diaphragm pump is used for driving a cleaning liquid to pass through the sampling needle from the clear liquid tank and be injected into the needle washing groove; and the second diaphragm pump is used for driving the cleaning liquid to be sucked out from the upper part of the needle washing groove and flow into the waste liquid tank.
Further preferably, the first diaphragm pump is connected to the clear liquid tank and the plunger pump through the liquid pipeline, and the clear liquid is injected into the sampling needle after passing through the plunger pump.
Still further preferably, the bottom of the needle washing groove is provided with a deep hole, and the diameter of the deep hole is slightly larger than the outer diameter of the sampling needle for accommodating the insertion of the sampling needle.
Still further preferably, the bottom of the needle washing groove is provided with a waste liquid hole, the waste liquid hole is connected with a waste liquid outlet, the waste liquid outlet is connected with the second diaphragm pump, and the waste liquid outlet is connected to the waste liquid tank through the liquid pipeline.
Preferably, the horizontal screw motor comprises a horizontal screw motor and a longitudinal screw motor which are orthogonally arranged to drive the sampling needle to respectively move transversely and longitudinally in the horizontal direction.
More preferably, the multi-channel dry-type fluoroimmunoassay analyzer comprises a sample feeding control plate, wherein the sampling needle is arranged on one side of the sample feeding control plate and can move longitudinally along the sample feeding control plate under the driving of the longitudinal screw rod motor.
Further preferably, the sample adding control board is connected with the transverse screw rod motor through a control board transmission belt, and can move transversely along a sample adding guide rail under the drive of the transverse screw rod motor.
Has the advantages that: the utility model discloses a sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure, sets up the liquid way system that the loading has the washing liquid, including the needle washing groove that is equipped with the washing liquid, the sampling needle accomplishes the sampling to the first sample liquid of the examination of awaiting measuring to pour into first testing reagent card back into, before removing the sample reagent pipe to the second sample liquid that is equipped with the examination of awaiting measuring, by horizontal lead screw motor and perpendicular lead screw motor drive, remove earlier and fall into the needle washing groove, wash the sampling needle by the washing liquid of washing in the needle groove. After the cleaning is finished, the vertical screw rod motor and the horizontal screw rod motor drive the sampling needle to move to the second sample reagent tube, new sample liquid is extracted, and the process is circulated. The utility model discloses a sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure, through setting up the needle washing groove, washs the sampling needle after a sampling, has both guaranteed that cross contamination can not take place between the different sample liquids, satisfies the requirement that detects and multinomial detection in batches, but the whole batch of detection of automatic execution again, the detection personnel's that has significantly reduced intensity of labour.
The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings, so as to fully understand the objects, the features and the effects of the present invention.
Drawings
Fig. 1 is a schematic diagram of the internal structure of the multi-channel dry-type fluorescence immunoassay analyzer according to the present invention at a first angle.
Fig. 2 is a schematic diagram of the internal structure of the second angle of the multi-channel dry-type fluorescence immunoassay analyzer of the present invention.
Fig. 3 is a schematic diagram of the structure of the liquid path system of the multi-channel dry-type fluorescence immunoassay analyzer of the present invention.
Fig. 4 is a partial enlarged view of the liquid path system of the multi-channel dry-type fluorescence immunoassay analyzer of the present invention.
Fig. 5 is a sectional view showing the structure of the needle washing groove of the multi-channel dry-type fluorescence immunoassay analyzer of the present invention.
In the figure, 2-card slot, 4-test tube slot, 10-sample reagent tube, 20-sampling needle, 21-sample adding control plate, 22-transverse screw motor, 23-longitudinal screw motor, 24-vertical screw motor, 26-control plate driving belt, 27-sample adding guide rail, 31-flexible tube, 32-plunger pump, 33-liquid channel pipeline, 34-clear liquid tank, 35-waste liquid tank, 36-needle washing tank, 37-diaphragm pump, 38-deep hole; 39-waste liquid hole; 40-waste liquid outlet.
Detailed Description
The utility model provides a sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure, for making the utility model discloses a purpose, technical scheme and effect are clearer, clear and definite, and it is right to refer to the drawing below and to lift the example the utility model discloses do further detailed description. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The utility model provides a structure is washd to sampling needle that multichannel dry-type fluorescence immunoassay appearance was used, in a preferred embodiment, as shown in FIG. 1, in one side of preceding lower part, be provided with test tube slot 4, be equipped with at least one sample reagent pipe 10 that waits to detect the sample, when carrying out the inspection, upright in proper order by operating personnel, the opening is put into upwards in test tube slot 4, send into multichannel dry-type fluorescence immunoassay appearance's inside.
The utility model discloses an inner structure of multichannel dry-type fluorescence immunoassay appearance, including each corresponding operating device and circuit structure to accomplish automatic sampling, application of sample and multichannel fluorescence detection function. Referring to fig. 1 and 2, the internal structure includes a card slot assembly, a sample feeding assembly, and a detection assembly. The working process is briefly described as follows:
as shown in fig. 1, the card slot assembly includes at least one card slot 2, and at least one test reagent card is vertically placed in the card slot 2. Specifically, the top surface of each detection reagent card is provided with a sample adding hole with a set diameter, and a sample to be detected of liquid can be injected into each detection reagent card through the sample adding hole and then subjected to subsequent fluorescence detection.
Before detection, an operator sequentially puts a plurality of empty clean detection reagent cards 2 on the clamping grooves 2, and the sampling holes are upward. During detection, the sample adding assembly extracts liquid of a sample to be detected from the sample reagent tube 10, then the liquid moves to the upper part of the detection reagent card 2, the sample to be detected is loaded in the detection reagent card 2 to be detected, finally, the detection assembly performs fluorescence detection on the detection reagent card 2, and the detection result is sent to a computer system for further processing. And the detection reagent card 2 which completes the detection can be pushed away from the card slot 2.
The sample application assembly includes a hollow sampling needle 20 and a motor for moving the sampling needle 20. Wherein the sampling needle 20 is suspended above the card slot assembly with the needle tip facing downward, as shown in fig. 2. The sampling needle 20 is connected to at least one horizontal screw motor, typically two orthogonally disposed motors driving horizontal movement, namely a lateral screw motor 22 and a longitudinal screw motor 23, such that the sampling needle 20 is horizontally movable, including between the sample reagent tube 10 and the detection reagent card 2, under the driving of the horizontal screw motor. In a preferred embodiment, the specific structure is as shown in fig. 2, the sampling needle 20 is disposed on one side of a sample-adding control board 21, and the sampling needle 20 can move horizontally along the longitudinal direction of the sample-adding control board 21 under the driving of a longitudinal screw motor 23. The sample loading control plate 21 can be driven by a transverse screw motor 22, for example, by a control plate driving belt 26, and can horizontally move along the sample loading guide rail 27. And, the sampling needle 20 is further connected to a vertical screw motor 24, and when the sampling needle 20 moves horizontally to a position right above the sample reagent tube 10, the vertical screw motor 24 drives the sampling needle 20 to descend until the needle tip is inserted below a certain height of the liquid level in the sample reagent tube 10. As shown in FIG. 3, the needle end of the sampling needle 20 is connected to a plunger pump 32 for sucking and pushing out the sample liquid through a flexible tube 31. After the needle is inserted into the sample reagent tube 10 below the liquid level, the plunger pump 32 operates to pump the sample liquid to be detected. After the suction is finished, the vertical screw motor 24 works, the sampling needle 20 is lifted, and then the sampling needle horizontally moves to a position right above a detection reagent card to be detected in the card slot 12 under the driving of the transverse screw motor 22 and the longitudinal screw motor 23.
At this time, the sampling needle 20 is driven by the vertical screw motor 24 to descend to be inserted into the sample adding hole, and then driven by the plunger pump 32 to push out the sample liquid to be detected and inject the sample liquid into the detection reagent card. Of course, as long as the position control is accurate, the sampling needle 20 may not be lowered into the sample addition hole, but the sample solution to be detected may be pushed out at a certain height, and then fall into the detection reagent card through the sample addition hole.
In consideration of the multi-sample and multi-channel detection, the sampling needle 20 is inserted into the sample reagent tubes 10 to extract the sample liquid for many times, which may result in cross contamination of different samples, so the multi-channel dry-type fluorescence immunoassay analyzer of the present invention further comprises a liquid path system, as shown in fig. 3, the liquid path system comprises a liquid path pipeline 33, and a clear liquid tank 34 for storing the cleaning liquid, a waste liquid tank 35 for storing the waste liquid, and a needle washing tank 36 for washing the sampling needle 20 are respectively connected to each other. Thus, after the sampling needle 20 finishes sampling a sample reagent tube 10 and injecting the sample reagent tube into a corresponding detection reagent card, the sample reagent tube moves into the needle washing groove 36, is driven by the vertical screw rod motor 24 to wash the external old sample liquid up and down for several times, and is driven by the plunger pump 32 to suck and push out the washing liquid for several times to wash the inside of the sampling needle 20. After the cleaning is completed and the needle washing groove 20 is left, the plunger pump 32 may further continue to suck and push air several times to dry the inside of the sampling needle 30. After the cleaning is completed, the sampling needle 20 is driven to move to the next sample reagent tube 10 to continue sampling.
In view of the fact that the cleaning liquid in the needle cleaning tank 36 will be contaminated after several times of cleaning, in a preferred embodiment, the liquid path system further comprises a diaphragm pump 37 for driving the flow of the cleaning liquid inside the liquid path pipe 33 from the cleaning liquid tank 34 to the needle cleaning tank 36 and from the needle cleaning tank 36 to the waste liquid tank 35 after the cleaning is completed.
In a more preferred embodiment, the diaphragm pumps 37 are provided in two, wherein the first diaphragm pump drives the cleaning liquid from the cleaning liquid tank 34, through the sampling needle 20, and into the needle washing tank 36; this cleans the lumen of the sampling needle 20. Specifically, as shown in the enlarged view of fig. 4, the first diaphragm pump connects the clear liquid tank 34 and the plunger pump 32 through the liquid path pipe 33, and after passing through the plunger pump 32, the cleaning liquid is injected into the sampling needle 20, and is injected into the needle washing groove 36 from the needle head of the sampling needle 20, and accumulates in the needle washing groove 36 until the cleaning liquid overflows the outside of the sampling needle 20, and at this time, the second diaphragm pump drives the cleaning liquid, sucks the cleaning liquid from the needle washing groove 36, and flows into the waste liquid tank 35, thereby completing the cleaning of the outside of the sampling needle 20. During the cleaning process, the cleaning liquid is preferably kept in a flowing state as described above, i.e. both said membrane pumps 37 are continuously operated to ensure the cleaning effect.
In a better embodiment, as shown in the cross-sectional view of fig. 5, the bottom of the needle washing groove 36 is provided with a deep hole 38 for inserting the sampling needle 20, and the diameter of the deep hole is slightly larger than the outer diameter of the sampling needle. The cleaning liquid is driven by the first diaphragm pump and is injected into the deep hole 38 from the needle head of the sampling needle 20, meanwhile, a waste liquid hole 39 is formed in the other side of the bottom, the waste liquid hole 39 is connected with a waste liquid outlet 40, the waste liquid outlet 40 is connected with a second diaphragm pump, and the second diaphragm pump sucks the cleaning liquid to leave the deep hole 38 and discharges the cleaning liquid into the waste liquid tank 35 through the liquid path pipeline 33. During the cleaning process, the flow direction of the cleaning liquid in the needle washing groove 36 is shown by the arrow in fig. 5. The deep hole 38 is arranged, so that the cleaning effect can be ensured on the basis of reducing the using amount of the cleaning solution.
To sum up, the utility model discloses a sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure, sets up the liquid path system who has the washing liquid, including the needle washing groove 36 that is equipped with the washing liquid, sampling needle 20 accomplishes the sampling to the first sample liquid of the examination of awaiting measuring to pour into first testing reagent card into back, before removing the sample reagent pipe 10 that is equipped with the second sample liquid of the examination of awaiting measuring, by horizontal lead screw motor and the drive of perpendicular lead screw motor 24, remove earlier and fall into needle washing groove 36, wash sampling needle 20 by the washing liquid of washing in the needle groove 36. After the cleaning is completed, the vertical screw motor 24 and the horizontal screw motor drive the sampling needle 20 to move to the second sample reagent tube, and a new sample liquid is extracted, and the process is circulated. The utility model discloses a sampling needle that multichannel dry-type fluorescence immunoassay appearance was used washs structure, through setting up needle washing groove 36, washs sampling needle 20 after a sampling, has both guaranteed that cross contamination can not take place between the different sample liquid, satisfies the requirement that detects and multinomial detection in batches, but the whole batch of detection of automatic execution again, the detection personnel's that has significantly reduced intensity of labour.
The foregoing has described in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be devised by those skilled in the art in light of the teachings of the present invention without undue experimentation. Therefore, the technical solutions that can be obtained by a person skilled in the art through logic analysis, reasoning or limited experiments based on the prior art according to the concepts of the present invention should be within the scope of protection defined by the claims.
Claims (9)
1. A sampling needle cleaning structure for a multi-channel dry-type fluorescence immunoassay analyzer is characterized in that the multi-channel dry-type fluorescence immunoassay analyzer comprises: the device comprises a test tube slot for placing a sample reagent tube and a clamping slot for placing a detection reagent card, wherein the orifice of the sample reagent tube is upwards placed on the test tube slot, and the top of the detection reagent card is provided with an opening with a set diameter;
the sampling device also comprises a hollow sampling needle, wherein the needle head of the sampling needle is downward and movably suspended above the test tube slot and the clamping slot;
the needle tail of the sampling needle is connected with a plunger pump for sucking and pushing out the sample liquid in the sampling needle;
the sampling needle is connected with at least one horizontal screw motor and can horizontally move under the driving of the horizontal screw motor;
the sampling needle is also connected with a vertical screw motor and can be lifted under the driving of the vertical screw motor;
the device also comprises a liquid path system loaded with cleaning liquid, which comprises a liquid path pipeline, a liquid cleaning tank used for storing clean cleaning liquid, a waste liquid tank used for storing cleaned waste liquid and a needle cleaning tank filled with cleaning liquid, wherein the cleaning liquid is used for cleaning the sampling needle.
2. The sampling needle cleaning structure according to claim 1, wherein the liquid path system further includes a diaphragm pump for driving a flow of a cleaning liquid inside the liquid path pipe, the diaphragm pump driving the cleaning liquid, flowing from the clear liquid tank into the needle wash tank, and flowing from the needle wash tank into the waste liquid tank.
3. The sampling needle cleaning structure according to claim 2, wherein the diaphragm pump includes two, a first diaphragm pump and a second diaphragm pump, the first diaphragm pump is used for driving a cleaning liquid to be injected into the needle washing groove from the clear liquid tank through the sampling needle; and the second diaphragm pump is used for driving the cleaning liquid to be sucked out from the upper part of the needle washing groove and flow into the waste liquid tank.
4. The sampling needle cleaning structure according to claim 3, wherein the first diaphragm pump connects the clear liquid tank and the plunger pump through the liquid path pipe, and clear liquid is injected into the sampling needle by the plunger pump.
5. The sampling needle cleaning structure according to claim 4, wherein a deep hole is formed at the bottom of the needle washing groove, and the diameter of the deep hole is slightly larger than the outer diameter of the sampling needle for accommodating the insertion of the sampling needle.
6. The cleaning structure for the sampling needle according to claim 5, wherein the bottom of the needle washing groove is further opened with a waste liquid hole, the waste liquid hole is connected with a waste liquid outlet, the waste liquid outlet is connected with the second diaphragm pump, and the waste liquid outlet is connected to the waste liquid tank through the liquid path pipeline.
7. The cleaning structure of the sampling needle according to any one of claims 1 to 6, wherein the horizontal screw motor comprises a horizontal screw motor and a longitudinal screw motor which are orthogonally arranged with each other, and drives the sampling needle to move laterally and longitudinally in the horizontal direction, respectively.
8. The cleaning structure of claim 7, wherein the multi-channel dry-type immunofluorometric analyzer comprises a sample application control plate, and the sampling needle is disposed at one side of the sample application control plate and can move longitudinally along the sample application control plate under the driving of the longitudinal screw motor.
9. The cleaning structure for the sampling needle according to claim 8, wherein the sample-adding control board is connected to the transverse screw motor through a control board transmission belt, and can move transversely along a sample-adding guide rail under the driving of the transverse screw motor.
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| CN202120806663.8U CN214585496U (en) | 2021-04-20 | 2021-04-20 | Sampling needle cleaning structure for multi-channel dry-type fluorescence immunoassay analyzer |
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| CN202120806663.8U CN214585496U (en) | 2021-04-20 | 2021-04-20 | Sampling needle cleaning structure for multi-channel dry-type fluorescence immunoassay analyzer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004040A (en) * | 2019-04-16 | 2019-07-12 | 北京龙基高科生物科技有限公司 | A kind of automatic loading system of gene sequencing |
| CN114377549A (en) * | 2022-01-18 | 2022-04-22 | 深圳汇芯生物医疗科技有限公司 | Automated separation apparatus and method for separating target particles in liquid sample |
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2021
- 2021-04-20 CN CN202120806663.8U patent/CN214585496U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004040A (en) * | 2019-04-16 | 2019-07-12 | 北京龙基高科生物科技有限公司 | A kind of automatic loading system of gene sequencing |
| CN114377549A (en) * | 2022-01-18 | 2022-04-22 | 深圳汇芯生物医疗科技有限公司 | Automated separation apparatus and method for separating target particles in liquid sample |
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