CN214571892U - Filter separator of mesenchymal stem cell - Google Patents
Filter separator of mesenchymal stem cell Download PDFInfo
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- CN214571892U CN214571892U CN202120030014.3U CN202120030014U CN214571892U CN 214571892 U CN214571892 U CN 214571892U CN 202120030014 U CN202120030014 U CN 202120030014U CN 214571892 U CN214571892 U CN 214571892U
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- 238000001914 filtration Methods 0.000 claims abstract description 76
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- 239000007921 spray Substances 0.000 claims abstract description 26
- 230000007246 mechanism Effects 0.000 claims abstract description 7
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Abstract
The utility model provides a filter mesenchymal stem cell's separator belongs to cell splitter field. The separator for filtering the mesenchymal stem cells comprises a container, a liquid circulation mechanism and a collection assembly. The opening at the upper end of the container is connected with a sealing cover. The liquid pumping end of the pump body is communicated with the bottom of the container, and the liquid discharging end of the pump body is communicated with the spray head. The filter screen is arranged in the collection screen, and the spray head is arranged in the filter screen; the liquid pumping end of the pump body is communicated with the bottom of the container, the liquid discharging end of the pump body is communicated with the spray head, the pump body is controlled to pump the solution filtered at the bottom of the container, and the solution is sprayed out through the spray head after pressurization, so that the pre-separation mixture in the filter screen is stirred, mixed and scattered, and the mesenchymal stem cells in the pre-separation mixture can pass through the second filter holes on the surface of the filter screen, so that the content of the mesenchymal stem cells in the pre-separation mixture is reduced, and the separation effect is improved.
Description
Technical Field
The utility model relates to a cell splitter field particularly, relates to a filter mesenchymal stem cells's separator.
Background
At present, mesenchymal stem cells are pluripotent stem cells that have all of the commonalities of stem cells, namely self-renewal and multipotential differentiation capacity; the clinical application is the most, and the combined application with hematopoietic stem cells can improve the success rate of transplantation and accelerate hematopoietic reconstruction; after a patient receives a large dose of chemotherapy, the mesenchymal stem cells and the hematopoietic stem cells are input together, so that the recovery time of the blood cells of the patient can be obviously accelerated, and the chemotherapy is safe and has no adverse reaction. Mesenchymal stem cells are present not only in the bone marrow but also in skeletal muscle, periosteum and trabecula. The mesenchymal stem cells are clinically applied to the aspects of solving various blood system diseases, cardiovascular diseases, liver cirrhosis, nervous system diseases, the repair of partial resection injury of knee joints, autoimmune diseases and the like, and make a major breakthrough, thereby saving the lives of more patients. In addition, the mesenchymal stem cells have long-term development prospect in the aspects of nervous system repair and more aspects;
the existing stem cell separator has the defects that the mixture in a conical stainless steel screen is easy to agglomerate due to the increase of viscosity, and the filtering and separation of mesenchymal stem cells are not facilitated.
SUMMERY OF THE UTILITY MODEL
In order to compensate the above deficiency, the utility model provides a filter mesenchymal stem cells's separator aims at improving stem cells's separator and easily takes place to reunite when filtering, is unfavorable for mesenchymal stem cells's the problem of filtering separation.
The utility model discloses a realize like this:
the utility model provides a filter separator of mesenchymal stem cells, including container, liquid circulation mechanism and collection subassembly.
The container upper end opening sets up, just container upper end opening part is connected with sealed lid.
The liquid circulation mechanism comprises a pump body and a spray head, wherein the liquid pumping end of the pump body is communicated with the bottom of the container, and the liquid discharging end of the pump body is communicated with the spray head.
The collecting assembly comprises a collecting screen and a filtering screen, the collecting screen is of a cylindrical structure with an upper end open, first filtering holes are formed in the side wall and the bottom of the collecting screen, the filtering screen is hemispherical, second filtering holes are formed in the surface of the filtering screen, the collecting screen is arranged in the container, the filtering screen is arranged in the collecting screen, and the spray head is arranged in the filtering screen.
In an embodiment of the present invention, the container is a bottle or a barrel, the bottom of the container is fixed with a base, the bottom surface of the base is pasted with a non-slip mat, and the bottom of the container is provided with a liquid outlet.
The utility model discloses an in one embodiment, sealed lid with container laminating junction is provided with the sealing washer, the atmospheric pressure balance mouth has been seted up at sealed lid top, atmospheric pressure balance mouth department spiro union has the end cap.
In an embodiment of the present invention, the container sidewall is embedded with an observation window, the edge of the sealing cover is fixed with a hook, the sidewall of the upper end of the container is fixed with a buckle, and the buckle is fixed with the matching joint of the hook.
In an embodiment of the present invention, the container sidewall has been provided with a first diversion hole, the upper end of the first diversion hole extends to the upper end face of the container, the lower end of the first diversion hole extends to the bottom of the container, the edge of the sealing cover has been provided with a second diversion hole, the lower end of the second diversion hole is correspondingly arranged on the upper end of the first diversion hole, and the upper end of the second diversion hole is connected to the liquid-drawing end of the pump body through a hard pipeline.
The utility model discloses an in one embodiment, sealed lid inner wall is fixed with the body, the upper end of body with the flowing back end of the pump body is connected, the lower extreme of body with the shower nozzle is connected, the shower nozzle is rotatory nozzle.
The utility model discloses an in the embodiment, sealed inslot wall connection has the splashproof screen cloth, the splashproof screen cloth cover in filter screen net upper surface, splashproof screen cloth lower surface with filter screen net upper surface edge sealing contact, the splashproof screen cloth surface has been seted up the third and has been filtered the hole.
The utility model discloses an in the embodiment, filter screen slides and cup joints the body surface, sealed lid inner wall fixedly connected with elastic component, the elastic component lower extreme with filter screen goes up fixed surface and connects, the elastic component can be the pressure spring.
The utility model discloses an in one embodiment, container upper end opening part is provided with the ladder groove, it is fixed with the spacing ring to collect screen cloth upper end, the spacing ring joint is in the ladder inslot, it is in to collect the unsettled setting in screen cloth bottom in the container.
The utility model discloses an in one embodiment, the spacing ring upper surface is seted up flutedly, filter screen cloth upper end edge joint is in the recess, the unsettled setting in filter screen cloth bottom is in collect in the screen cloth.
The utility model has the advantages that: the utility model discloses a separator of filtration mesenchymal stem cell that above-mentioned design obtained, during the use, liquid phase in the preseparation mixture is filtering screen cloth in proper order and is collected the screen cloth, enter into the container lower part, the drawing liquid end and the container bottom intercommunication of the pump body, the flowing back end and the shower nozzle intercommunication of the pump body, the solution after the filtration of control pump body extraction container bottom, spray through the shower nozzle after the pressurization, the realization is to the stirring of preseparation mixture in the filtering screen cloth, mix, break up, at the stirring of preseparation mixture, mix, break up the in-process, the mesenchymal stem cell in the preseparation mixture can pass through from the second filtration pore of filtering screen cloth surface, mesenchymal stem cell's separation has been realized, the content of mesenchymal stem cell in the preseparation mixture has been reduced, the separation effect has been improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic view of an external structure of a container according to an embodiment of the present invention;
fig. 2 is a schematic perspective view of a container according to an embodiment of the present invention;
fig. 3 is a schematic view of a seal cover according to an embodiment of the present invention;
fig. 4 is a schematic view of a container cutting structure according to an embodiment of the present invention.
In the figure: 100. a container; 110. a sealing cover; 111. an air pressure balancing port; 113. a hook; 115. a second flow guide hole; 117. a rigid pipe; 119. an elastic member; 130. a liquid discharge port; 150. an observation window; 170. buckling; 190. a first flow guide hole; 300. a liquid circulation mechanism; 310. a pump body; 330. a spray head; 350. a pipe body; 500. a collection assembly; 510. collecting the screen; 530. filtering the screen; 550. a splash screen.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the utility model provides a. Embodiments, all other embodiments obtained by a person skilled in the art without any inventive step are within the scope of the present invention.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
Examples
Referring to fig. 1-4, the present invention provides a technical solution: a separator for filtering mesenchymal stem cells includes a container 100, a liquid circulation mechanism 300, and a collection assembly 500.
Referring to fig. 1-4, an opening is formed at the upper end of the container 100, and a sealing cap 110 is connected to the opening at the upper end of the container 100;
in this embodiment, the container 100 is a bottle or a barrel, a base is fixed at the bottom of the container 100, a non-slip mat is adhered to the lower surface of the base, and a liquid outlet 130 is formed at the bottom of the container 100, so that the container 100 can be stably placed on the surface of a test bed.
In some embodiments, a sealing ring is disposed at a joint of the sealing cover 110 and the container 100, an air pressure balancing port 111 is formed at the top of the sealing cover 110, and a plug is screwed at a port of the air pressure balancing port 111 to improve a sealing effect of the joint.
It should be noted that, the observation window 150 is embedded in the side wall of the container 100, which is convenient for observing the separation condition inside, the hook 113 is fixed at the edge of the sealing cover 110, the buckle 170 is fixed on the side wall of the upper end of the container 100, the buckle 170 and the hook 113 are matched and clamped for fixation, which is convenient for the sealing cover 110 and the container 100 to be connected, sealed and detached.
In other embodiments, the sidewall of the container 100 is provided with a first guiding hole 190, the upper end of the first guiding hole 190 extends to the upper end surface of the container 100, the lower end of the first guiding hole 190 extends to the bottom of the container 100, the edge of the sealing cover 110 is provided with a second guiding hole 115, the lower end of the second guiding hole 115 is arranged corresponding to the upper end of the first guiding hole 190, and the upper end of the second guiding hole 115 is connected with the liquid-extracting end of the pump body 310 through a hard pipeline 117; the lower end of the second flow guide hole 115 is provided with a sealing pad at the joint with the upper end of the first flow guide hole 190, the hard pipeline 117 is fixed on the upper end surface of the sealing cover 110, the first flow guide hole 190 formed through the container 100 and the second flow guide hole 115 formed through the sealing cover 110 are mutually matched and communicated, so that the solution at the bottom of the container 100 can be conveniently pumped to the upper part, and the hard pipeline 117 is fixed on the upper end surface of the sealing cover 110, is not only used for flow guide, but also is convenient to hold by hand, and plays a role of a handle.
Referring to fig. 3, the liquid circulation mechanism 300 includes a pump body 310 and a spray head 330, wherein a liquid suction end of the pump body 310 is communicated with the bottom of the container 100, and a liquid discharge end of the pump body 310 is communicated with the spray head 330;
in this embodiment, a tube 350 is fixed on the inner wall of the sealing cap 110, the upper end of the tube 350 is connected to the liquid discharge end of the pump body 310, the lower end of the tube 350 is connected to the spray head 330, the spray head 330 is a rotary spray head, the liquid discharge end of the pump body 310 is connected to the spray head 330, and the spray head 330 sprays liquid to stir, mix and scatter the cell sap in the filter screen 530, thereby reducing the adhesion and agglomeration of the filter screen 530; the mesenchymal stem cells are conveniently separated out through the filtering mesh 530.
Referring to fig. 4, the collection assembly 500 includes a collection screen 510 and a filtering screen 530, the collection screen 510 is a cylindrical structure with an open upper end, the side wall and the bottom of the collection screen 510 are both provided with first filtering holes, the filtering screen 530 is hemispherical, the surface of the filtering screen 530 is provided with second filtering holes, the collection screen 510 is disposed in the container 100, the filtering screen 530 is disposed in the collection screen 510, the spray head 330 is disposed in the filtering screen 530, the filtering screen 530 is used for filtering other substances with a particle size larger than that of the mesenchymal stem cells, the hole size of the second filtering holes is 25 μm, the mesenchymal stem cells are allowed to pass through but other substances with a particle size larger than that of the mesenchymal stem cells are not allowed to pass through, thereby realizing the separation of the mesenchymal stem cells.
In this embodiment, the inner wall of the sealing cover 110 is connected with a splash-proof screen 550, the splash-proof screen 550 covers the upper surface of the filtering screen 530, the lower surface of the splash-proof screen 550 is in sealing contact with the edge of the upper surface of the filtering screen 530, the surface of the splash-proof screen 550 is provided with a third filtering hole, the aperture of the third filtering hole is 6 μm, in some embodiments, the filtering screen 530 is slidably sleeved on the surface of the pipe 350, the inner wall of the sealing cover 110 is fixedly connected with an elastic member 119, the lower end of the elastic member 119 is fixedly connected with the upper surface of the filtering screen 530, and the elastic member 119 may be a compression spring; allowing the liquid phase in the pre-separation mixture to pass through, but not allowing the mesenchymal stem cells in the pre-separation mixture to pass through, thereby not only being beneficial to the mixing, stirring and passing of the liquid phase in the pre-separation mixture, but also being capable of effectively preventing the mesenchymal stem cells from dissipating and losing in the stirring process, and further reducing the loss rate of the mesenchymal stem cells; since the aperture of the collection screen 510 is small, only the liquid phase can pass through, the mesenchymal stem cells are not allowed to pass through, but the liquid phase is allowed to pass through the collection screen 510, so that the mesenchymal stem cells are all trapped in the collection screen 510, the separation time of the mesenchymal stem cells is shortened, and the separation efficiency is improved.
It should be noted that a stepped groove is formed at an opening at the upper end of the container 100, a limiting ring is fixed at the upper end of the collecting screen 510, the limiting ring is clamped in the stepped groove, and the bottom of the collecting screen 510 is suspended in the container 100; in specific implementation, a groove is formed in the upper surface of the limiting ring, the edge of the upper end of the filtering screen 530 is clamped in the groove, and the bottom of the filtering screen 530 is suspended in the collecting screen 510; the filtering screen 530 is clamped at the upper end of the collecting screen 510, so that the filtering screen is convenient to replace, the mesenchymal stem cells or/and other cells or units with different particle sizes can be separated, tissue blocks and/or impurities with larger particle sizes are retained, and the yield and purity of the mesenchymal stem cells obtained by the separation are improved; the collection screen 510 is clamped in the container 100, so that the collection screen 510 is convenient to replace, and the collection screen 510 is changed to intercept mesenchymal stem cells with different particle sizes, so that the application range of the mesenchymal stem cell separator is expanded; the first filtering hole has a pore size of 7 μm, does not allow mesenchymal stem cells to pass through but allows a liquid phase to pass through the collection screen 510.
Each part can be dismantled respectively, has made things convenient for disinfection, washing and possible maintenance, the complete separation of mesenchymal stem cell is accomplished jointly in coordination among each system, liquid circulation system can realize the homodisperse of pre-separation mixture, increases the discipline that mesenchymal stem cell wherein passes through from filtration system, and the separation degree is higher, and the mesenchymal stem cell purity of gained is better to need not to carry out purification separation once more to the mesenchymal stem cell who separates and can use, separator convenient operation, and the application of being convenient for.
The working principle of the separator for filtering the mesenchymal stem cells is as follows: when in use, the parts are sterilized respectively, then the filtering screen 530 and the collecting screen 510 are arranged in the container 100 under the aseptic condition, the pre-separation mixture is guided into the filtering screen 530, and the opening at the upper end of the container 100 is connected with the sealing cover 110 for sealing the container 100. The collecting screen 510 is a cylindrical structure with an opening at the upper end, so that stem cells can be conveniently collected, first filtering holes are formed in the side wall and the bottom of the collecting screen 510 and used for filtering liquid in a pre-separation mixture, and the filtering screen 530 is hemispherical, so that the filtering area is increased; and the surface of the filtering screen 530 is provided with second filtering holes, the mesenchymal stem cells pass through the filtering screen 530, the liquid phase in the pre-separation mixture sequentially passes through the filtering screen 530 and the collecting screen 510 and enters the lower part of the container 100, the liquid pumping end of the pump body 310 is communicated with the bottom of the container 100, the liquid discharging end of the pump body 310 is communicated with the spray head 330, the pump body 310 is controlled to pump the solution filtered at the bottom of the container 100 and spray the solution through the spray head 330 after pressurization, so that the pre-separation mixture in the filtering screen 530 is stirred, mixed and dispersed, and the mesenchymal stem cells in the pre-separation mixture can pass through the second filtering holes on the surface of the filtering screen 530 in the stirring, mixing and dispersing processes of the pre-separation mixture, so that the separation of the mesenchymal stem cells is realized, the content of the mesenchymal stem cells in the pre-separation mixture is reduced, and the separation effect is improved.
It should be noted that the specific model specifications of the pump body 310 and the nozzle 330 need to be determined by type selection according to the actual specification of the device, and the specific type selection calculation method adopts the prior art, so detailed description is omitted.
The power supply of the pump body 310 and its principle will be clear to a person skilled in the art and will not be described in detail here.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A separator for filtering mesenchymal stem cells, comprising
The container (100), the upper end opening of the container (100) is arranged, and the upper end opening of the container (100) is connected with a sealing cover (110);
the liquid circulation mechanism (300) comprises a pump body (310) and a spray head (330), the liquid suction end of the pump body (310) is communicated with the bottom of the container (100), and the liquid discharge end of the pump body (310) is communicated with the spray head (330);
collect subassembly (500), collect subassembly (500) including collecting screen cloth (510) and filtering screen cloth (530), collect screen cloth (510) and be upper end open-ended tubular structure, just first filtration hole has all been seted up to the lateral wall and the bottom of collecting screen cloth (510), filtering screen cloth (530) are the hemisphere, just filtering screen cloth (530) surface has seted up the second and has filtered the hole, collect screen cloth (510) and set up in container (100), filtering screen cloth (530) set up collect in the screen cloth (510), shower nozzle (330) set up in filtering screen cloth (530).
2. The mesenchymal stem cell filtering separator according to claim 1, wherein the container (100) is a bottle or a barrel, a base is fixed at the bottom of the container (100), an anti-slip pad is adhered to the lower surface of the base, and a liquid outlet (130) is formed at the bottom of the container (100).
3. The mesenchymal stem cell filtering separator according to claim 1, wherein a sealing ring is disposed at a joint of the sealing cover (110) and the container (100), an air pressure balancing port (111) is formed at the top of the sealing cover (110), and a plug is screwed at a port of the air pressure balancing port (111).
4. The mesenchymal stem cell filtering separator according to claim 1, wherein the container (100) is embedded with an observation window (150) on a side wall thereof, the sealing cover (110) is fixed with a hook (113) on an edge thereof, the container (100) is fixed with a buckle (170) on an upper side wall thereof, and the buckle (170) is matched with and clamped with the hook (113) for fixation.
5. The mesenchymal stem cell filtering separator according to claim 1, wherein a first guiding hole (190) is formed in a side wall of the container (100), an upper end of the first guiding hole (190) extends to an upper end surface of the container (100), a lower end of the first guiding hole (190) extends to a bottom of the container (100), a second guiding hole (115) is formed in an edge of the sealing cover (110), a lower end of the second guiding hole (115) is disposed corresponding to an upper end of the first guiding hole (190), and an upper end of the second guiding hole (115) is connected to a liquid pumping end of the pump body (310) through a rigid pipe (117).
6. The mesenchymal stem cell filtering separator according to claim 1, wherein a tube (350) is fixed to an inner wall of the sealing cover (110), an upper end of the tube (350) is connected to a drainage end of the pump body (310), a lower end of the tube (350) is connected to the spray head (330), and the spray head (330) is a rotary spray head.
7. The mesenchymal stem cell filtering separator as claimed in claim 1, wherein a splash screen (550) is connected to an inner wall of the sealing cover (110), the splash screen (550) covers an upper surface of the filtering screen (530), a lower surface of the splash screen (550) is in sealing contact with an edge of the upper surface of the filtering screen (530), and a third filtering hole is formed in a surface of the splash screen (550).
8. The mesenchymal stem cell filter according to claim 6, wherein the filter screen (530) is slidably sleeved on the surface of the tube (350), an elastic member (119) is fixedly connected to the inner wall of the sealing cover (110), the lower end of the elastic member (119) is fixedly connected to the upper surface of the filter screen (530), and the elastic member (119) may be a compression spring.
9. The mesenchymal stem cell filtering separator according to claim 1, wherein a stepped groove is formed at an opening at the upper end of the container (100), a limiting ring is fixed at the upper end of the collecting screen (510), the limiting ring is clamped in the stepped groove, and the bottom of the collecting screen (510) is suspended in the container (100).
10. The mesenchymal stem cell filtering separator as claimed in claim 9, wherein the upper surface of the limiting ring is provided with a groove, the upper edge of the filtering screen (530) is clamped in the groove, and the bottom of the filtering screen (530) is suspended in the collecting screen (510).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120030014.3U CN214571892U (en) | 2021-01-06 | 2021-01-06 | Filter separator of mesenchymal stem cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120030014.3U CN214571892U (en) | 2021-01-06 | 2021-01-06 | Filter separator of mesenchymal stem cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214571892U true CN214571892U (en) | 2021-11-02 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120030014.3U Active CN214571892U (en) | 2021-01-06 | 2021-01-06 | Filter separator of mesenchymal stem cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214571892U (en) |
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2021
- 2021-01-06 CN CN202120030014.3U patent/CN214571892U/en active Active
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Address after: Room 101, Building 10, No. 365 Changjiang Road, Changhe Street, Binjiang District, Hangzhou City, Zhejiang Province 310000 Patentee after: Weiwei (Hangzhou) Regenerative Medicine Technology Co.,Ltd. Country or region after: China Address before: 310000 Room 201, 2 / F, building 2, 288 Qiuyi Road, Changhe street, Binjiang District, Hangzhou City, Zhejiang Province Patentee before: ZHEJIANG HEALTHFUTURE BIOMEDICAL TECHNOLOGY CO.,LTD. Country or region before: China |