CN219099149U - Device for quickly separating cells after digestion of tissue blocks - Google Patents
Device for quickly separating cells after digestion of tissue blocks Download PDFInfo
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- CN219099149U CN219099149U CN202320065143.5U CN202320065143U CN219099149U CN 219099149 U CN219099149 U CN 219099149U CN 202320065143 U CN202320065143 U CN 202320065143U CN 219099149 U CN219099149 U CN 219099149U
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- filter screen
- cell filter
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Abstract
The utility model relates to a device for quickly separating cells after digestion of tissue blocks, which comprises a tube body, a cell filter screen assembly, a grinding rod and a negative pressure pump, wherein the top of the tube body is provided with an opening, and the side part of the tube body is provided with a suction filtration port; the cell filter screen assembly comprises an upper filter cartridge and a lower filter cartridge, the upper filter cartridge comprises an upper cylinder body and an upper cell filter screen, the top of the upper cylinder body is detachably connected to the opening of the pipe body, the upper cell filter screen is connected to the bottom of the upper cylinder body, and the upper cell filter screen is in a downward bent arc shape; the lower filter cylinder comprises a lower cylinder body and a lower cell filter screen, the top of the lower cylinder body is detachably connected to the upper cylinder body, the lower cell filter screen is connected to the bottom of the lower cylinder body, and the mesh number of the upper cell filter screen is smaller than that of the lower cell filter screen; the grinding rod is used for grinding the tissue blocks on the upper cell filter screen; the negative pressure pump is communicated with the suction filtration port of the pipe body. The method can efficiently and conveniently separate the cells obtained after the digestion of the tissue blocks, has higher cell yield and purer separated cells.
Description
Technical Field
The utility model belongs to the technical field of biomedical equipment, and particularly relates to a device for quickly separating cells after digestion of tissue blocks.
Background
When tissue digestion is carried out, the tissue block is complex in composition and also contains a large amount of connective tissue, the tissue block cannot be completely digested into loose cells by single pancreatin digestion or combined digestion of multiple collagenases, undigested connective tissue still exists in the middle of the cells to connect the cells into a group, and the cell group easily blocks the holes of a cell sieve, so that the cells to be separated cannot easily pass through the cell sieve, and the collection of the cells becomes difficult. In order to obtain more cells, the tissue blocks which are not completely digested on the cell filter screen are usually ground and washed, the ground tissue blocks become more adhered, and the cell filter screen is less likely to filter the ground cells.
The cell filter screens in the current market are all single-layer stainless steel net-shaped or nylon net-shaped cell filter screens, and when the filter screens are used for separating cells after digestion of tissue blocks, the separation is insufficient and even difficult to separate. In addition, the cell screen is usually fixedly arranged on the centrifuge tube, and the subsequent cleaning and replacement are very inconvenient.
Disclosure of Invention
The utility model aims to provide a device for quickly separating cells after digestion of tissue blocks.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a device for rapid separation of cells following digestion of a tissue mass, comprising:
tube body: the top of the pipe body is provided with an opening, and the side part of the pipe body is provided with a suction filtration port;
cell screen assembly: the cell filter screen component comprises an upper filter cartridge and a lower filter cartridge, the upper filter cartridge comprises an upper cylinder body and an upper cell filter screen, the top of the upper cylinder body is detachably connected to the opening of the pipe body, the upper cell filter screen is connected to the bottom of the upper cylinder body, and the upper cell filter screen is in a downward bent arc shape; the lower filter cylinder comprises a lower cylinder body and a lower cell filter screen, the top of the lower cylinder body is detachably connected to the upper cylinder body, the lower cell filter screen is connected to the bottom of the lower cylinder body, and the mesh number of the upper cell filter screen is smaller than that of the lower cell filter screen;
grinding rod: the grinding rod is used for grinding tissue blocks on the upper cell filter screen;
negative pressure pump: the negative pressure pump is communicated with the suction filtration port of the pipe body.
According to the technical scheme, preferably, the top of the upper cylinder body is provided with the flanging, and the flanging is supported at the opening of the pipe body.
Further preferably, the inner side surface of the flange is provided with an internal thread, the outer surface of the opening of the pipe body is provided with an external thread, and the flange is supported at the opening of the pipe body and is in threaded connection with the pipe body.
According to the technical scheme, preferably, the opening of the pipe body is provided with the pipe cover, and when the cell filter screen assembly is detached from the pipe body, the pipe cover can be covered at the opening of the pipe body.
Preferably, the lower part of the upper cylinder is provided with a connecting part connected with the lower cylinder, the outer surface of the connecting part is provided with external threads, the inner surface of the top of the lower cylinder is provided with internal threads, and the top of the lower cylinder is in threaded connection with the connecting part of the upper cylinder.
Further preferably, the outer diameter of the connecting portion of the upper cylinder is smaller than the outer diameter of other portions of the upper cylinder.
Preferably, the upper cell filter screen is a nylon net film, the mesh number of the upper cell filter screen is 200-220 mesh, and/or,
the lower cell filter screen is a nylon net film, and the mesh number of the lower cell filter screen is 350-380 meshes.
According to the technical scheme, preferably, the suction filtration port of the pipe body is provided with the sealing cap, and when the negative pressure pump is detached from the suction filtration port of the pipe body, the sealing cap can be covered at the suction filtration port of the pipe body.
In the above technical solution, preferably, the outer circumferential surface of the tube body is provided with scales, and/or,
the bottom of the pipe body is conical.
In the above technical solution, preferably, one end of the grinding rod forms a grinding end, and the grinding end is provided with a rubber head.
Due to the application of the technical scheme, compared with the prior art, the utility model has the following advantages:
1. the upper cell filter screen can separate undigested connective tissues with larger volume and the like, loose cells can pass through the cell filter screen with enough space, and meanwhile, the upper cell filter screen is in a downward bent arc shape, so that digested tissue blocks and cells are conveniently concentrated in the middle of the cell filter screen, the grinding is convenient, the cell loss after grinding is reduced, and the cell yield is improved;
2. the lower cell filter screen further separates cells passing through the upper cell filter screen, and blocks some undigested tissue fragments and the like, so that the separated cells are relatively pure;
3. the tube body, the upper filter cylinder and the lower filter cylinder are of detachable structures, so that the upper cell filter screen and the lower cell filter screen can be combined by themselves, and the cell filter screens are selected in a targeted manner according to the required cell size, so that the efficiency of the separated target cells is higher;
4. when the whole device is used for separating cells, the negative pressure pump can enable the inside of the tube body to be in a negative pressure state, so that digested cells are accelerated to enter the tube body through the cell filter screen, and the waiting time for cell separation is shortened.
Drawings
FIG. 1 is a schematic diagram of the structure of the device of the present utility model;
FIG. 2 is a schematic top view of the upper cartridge of the present utility model;
fig. 3 is a schematic top view of the lower cartridge of the present utility model.
In the above figures:
1. a tube body; 10. a suction filtration port;
200. an upper cylinder; 201. an upper cell filter screen; 202. flanging; 203. a connection part;
210. a lower cylinder; 211. a lower cell filter screen;
3. a grinding rod; 30. a rubber head;
4. a negative pressure pump.
Detailed Description
The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
A device for rapid separation of cells after digestion of tissue mass, as shown in fig. 1, comprises a tube 1, a cell screen assembly, a grinding rod 3, and a negative pressure pump 4. The respective components are described in detail below.
The tube body 1 is cylindrical, the bottom is conical, a centrifuge tube made of polypropylene PP material can be adopted, the separation condition can be observed conveniently, and the specification is 50ml, 100ml, 150ml and the like. The top of the pipe body 1 is provided with an opening, the side part is provided with a suction filtration port 10, and the outer surface of the opening of the pipe body 1 is provided with external threads. The opening of the pipe body 1 is provided with a pipe cover, the suction filtration port 10 of the pipe body 1 is provided with a rubber sealing cap, when the pipe body 1 is not used, the pipe cover can be arranged at the opening of the pipe body 1 in a covering manner, and the sealing cap can be arranged at the suction filtration port 10 of the pipe body 1 in a covering manner. Furthermore, the outer peripheral surface of the tube body 1 is provided with graduations, and white areas are arranged for marking.
The cell filter screen component comprises an upper filter cartridge and a lower filter cartridge, and the pipe body 1, the upper filter cartridge and the lower filter cartridge are detachably connected. The specific method is as follows:
the upper cartridge comprises an upper cylinder 200 and an upper cell screen 201. Wherein: the upper cylinder 200 is cylindrical with two open ends, the top of the upper cylinder 200 is provided with a flange 202, the flange 202 is supported at the opening of the pipe body 1, meanwhile, the inner side surface of the flange 202 is provided with internal threads, and the flange 202 is supported at the opening of the pipe body 1 and is in threaded fit with the external threads of the pipe orifice of the pipe body 1, so that the sealing effect is achieved while the connection is stable; the lower part of the upper cylinder 200 has a connection part 203 connected with the lower filter cartridge, and the outer diameter of the connection part 203 is smaller than that of other parts of the upper cylinder 200, and the outer surface of the connection part 203 is provided with external threads. The upper cell filter 201 is connected to the bottom of the upper cylinder 200, and the upper cell filter 201 is in a downward curved arc shape, so that undigested tissue blocks are conveniently concentrated in the center of the cell filter by the arc-shaped upper cell filter 201, cell loss caused by grinding can be reduced, and loose cells can pass through the cell filter in a sufficient space. The upper cell filter screen 201 is a nylon net film, the mesh number of the upper cell filter screen 201 is 200-220 meshes, and undigested connective tissues with larger volume can be blocked.
The lower cartridge includes a lower cylinder 210, a lower cell screen 211. Wherein: the lower cylinder 210 is cylindrical with two open ends, an internal thread is provided on the inner surface of the top of the lower cylinder 210, the top of the lower cylinder 210 is sleeved on the connecting part 203 of the upper cylinder 200, and the two are in threaded fit. The lower cell filter screen 211 is connected to the bottom of the lower cylinder 210, the lower cell filter screen 211 is a nylon mesh membrane, the mesh number of the lower cell filter screen 211 is 350-380 mesh, the lower cell filter screen 211 further separates cells passing through the upper cell filter screen 201, and blocks some undigested tissue fragments and the like, so that the separated cells are relatively pure, and the separated cells are stored at the bottom of the tube body 1.
After connection, the pipe body 1, the upper filter cylinder and the lower filter cylinder are in threaded fit, so that the sealing in the pipe body 1 is ensured, on one hand, negative pressure is conveniently pumped, and on the other hand, bacteria invasion in the separation process is prevented.
The grinding rod 3 is cylindrical and is used for grinding tissue blocks on the upper cell filter screen 201, one end of the grinding rod 3 forms a grinding end, and the grinding end is provided with a rubber head 30.
The negative pressure pump 4 adopts a micro negative pressure pump, is communicated with the suction filtration port 10 of the pipe body 1 to absorb the bottom of the pipe body 1 into a negative pressure state, and the suction filtration port 10 is positioned below a cell filter screen component connected in the pipe body 1. The negative pressure pump 4 facilitates rapid separation, and the micro negative pressure state does not cause damage to cells like centrifugal separation.
When in use, the tube cover of the opening of the tube body 1 and the sealing cap of the suction filtration port 10 are disassembled, the lower cylinder 210 is screwed on the connecting part 203 of the upper cylinder 200, and then the upper cylinder 200 connected with the lower filter cylinder is supported at the opening of the tube body 1 through the flange 202 and screwed; the negative pressure pump 4 is connected, digested cell liquid is poured into the upper cell filter screen 201, and after the cell liquid is ground by the grinding rod 3, the negative pressure pump 4 is turned on, so that the cell liquid can be separated quickly and fully.
The above embodiments are provided to illustrate the technical concept and features of the present utility model and are intended to enable those skilled in the art to understand the content of the present utility model and implement the same, and are not intended to limit the scope of the present utility model. All equivalent changes or modifications made in accordance with the spirit of the present utility model should be construed to be included in the scope of the present utility model.
Claims (10)
1. A device for rapidly separating cells after digestion of a tissue mass, characterized by: comprising the following steps:
tube body: the top of the pipe body is provided with an opening, and the side part of the pipe body is provided with a suction filtration port;
cell screen assembly: the cell filter screen component comprises an upper filter cartridge and a lower filter cartridge, the upper filter cartridge comprises an upper cylinder body and an upper cell filter screen, the top of the upper cylinder body is detachably connected to the opening of the pipe body, the upper cell filter screen is connected to the bottom of the upper cylinder body, and the upper cell filter screen is in a downward bent arc shape; the lower filter cylinder comprises a lower cylinder body and a lower cell filter screen, the top of the lower cylinder body is detachably connected to the upper cylinder body, the lower cell filter screen is connected to the bottom of the lower cylinder body, and the mesh number of the upper cell filter screen is smaller than that of the lower cell filter screen;
grinding rod: the grinding rod is used for grinding tissue blocks on the upper cell filter screen;
negative pressure pump: the negative pressure pump is communicated with the suction filtration port of the pipe body.
2. The device for rapid separation of cells after digestion of tissue mass according to claim 1, wherein: the top of the upper cylinder body is provided with a flanging which is supported at the opening of the pipe body.
3. The device for rapid separation of cells after digestion of tissue mass according to claim 2, wherein: the inner side surface of the flanging is provided with an internal thread, the outer surface of the opening of the pipe body is provided with an external thread, and the flanging is supported at the opening of the pipe body and is in threaded connection with the pipe body.
4. A device for rapid separation of cells after digestion of tissue mass according to claim 1 or 3, wherein: the opening of the tube body is provided with a tube cover, and when the cell filter screen component is detached from the tube body, the tube cover can be covered at the opening of the tube body.
5. The device for rapid separation of cells after digestion of tissue mass according to claim 1, wherein: the lower part of the upper cylinder body is provided with a connecting part connected with the lower cylinder body, the outer surface of the connecting part is provided with external threads, the inner surface of the top of the lower cylinder body is provided with internal threads, and the top of the lower cylinder body is in threaded connection with the connecting part of the upper cylinder body.
6. The device for rapid separation of cells after digestion of tissue mass of claim 5, wherein: the outer diameter of the connecting part of the upper cylinder is smaller than the outer diameter of other parts of the upper cylinder.
7. The device for rapid separation of cells after digestion of tissue mass according to claim 1, wherein: the upper cell filter screen is a nylon net film, the mesh number of the upper cell filter screen is 200-220 meshes, and/or,
the lower cell filter screen is a nylon net film, and the mesh number of the lower cell filter screen is 350-380 meshes.
8. The device for rapid separation of cells after digestion of tissue mass according to claim 1, wherein: the suction port of the pipe body is provided with a sealing cap, and when the negative pressure pump is detached from the suction port of the pipe body, the sealing cap can be covered and arranged at the suction port of the pipe body.
9. The device for rapid separation of cells after digestion of tissue mass according to claim 1, wherein: the outer circumferential surface of the tube body is provided with scales and/or,
the bottom of the pipe body is conical.
10. The device for rapid separation of cells after digestion of tissue mass according to claim 1, wherein: one end of the grinding rod forms a grinding end, and the grinding end is provided with a rubber head.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320065143.5U CN219099149U (en) | 2023-01-10 | 2023-01-10 | Device for quickly separating cells after digestion of tissue blocks |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320065143.5U CN219099149U (en) | 2023-01-10 | 2023-01-10 | Device for quickly separating cells after digestion of tissue blocks |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219099149U true CN219099149U (en) | 2023-05-30 |
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ID=86429815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320065143.5U Active CN219099149U (en) | 2023-01-10 | 2023-01-10 | Device for quickly separating cells after digestion of tissue blocks |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219099149U (en) |
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2023
- 2023-01-10 CN CN202320065143.5U patent/CN219099149U/en active Active
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