CN210394361U - Filter membrane cover capable of obtaining supernatant of clarified cells - Google Patents

Abstract

The utility model discloses a filter membrane cover capable of obtaining clarified cell supernatant, which comprises a screen mechanism and a barrel body, wherein the barrel body is of a round tube type structure, the top end of the outer circular surface of the barrel body is uniformly provided with a plurality of buckles, the bottom end of the barrel body is communicated with the screen mechanism, and the barrel body is made of native polystyrene or stainless steel; the screen mechanism comprises a snap ring and a screen body, a filter cavity matched with the screen body is arranged at the bottom end of the barrel body, the filter cavity is communicated with the barrel body through a through hole, and the screen body is placed in the snap ring of the filter cavity; the utility model feeds the screen mechanism into the culture bottle through the cylinder body, and is clamped at the culture bottle opening through the buckle, so as to realize the fixation of the cylinder body, and finally, the pipettor directly extends into the cylinder body to absorb the supernatant filtered by the screen mechanism, so that the whole structure is simple, the operation is convenient, and the clarified supernatant can be conveniently taken out from the culture bottle at any time; and utilize screen cloth mechanism to filter, can effectual assurance supernatant's quality.

Description

Filter membrane cover capable of obtaining supernatant of clarified cells

Technical Field

The utility model belongs to the technical field of cell supernatant filters technique and specifically relates to a can acquire filtering membrane lid of clarification cell supernatant.

Background

Cell debris, impurities and other experimental non-useful substances are generated in the process of cell culture, and clarification of cell supernatant is the first step of downstream purification in the biopharmaceutical process. In order to obtain a clarified cell supernatant, a centrifuge is generally used at present to separate cells, cell debris and other impurities into a precipitate by the action of centrifugal force, and the supernatant is removed to achieve the purpose of obtaining the clarified supernatant. In the method, the effective components in the supernatant can be separated together when the centrifugal force is too large, and cell fragments, impurities and the like can be stored in the supernatant when the centrifugal force is too small, so that the effect of clarification cannot be achieved.

The utility model provides a can acquire filter membrane lid of clarification cell supernatant for replace the cell culture bottle lid, lid in culture flask mouth department, locate the suction with liquid from this filter membrane lid, the liquid that obtains is the clarification supernatant that does not contain cell, cell piece and other impurity promptly.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a can acquire the filter membrane lid of clarification cell supernatant to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

a can obtain the filter membrane lid of the supernatant fluid of the clarified cell, including screen cloth mechanism and cylinder, the cylinder is the round tubular structure, the cylinder is the even buckle that is equipped with of excircle surface top, the bottom link up of the cylinder connects the screen cloth mechanism, the length of the cylinder is not less than 10cm, the said cylinder chooses the native polystyrene material or stainless steel material, after the cultured cell of flask is ripe, when needing to collect the supernatant fluid, under the aseptic environment, open the bottle lid of the culture flask, then send the screen cloth mechanism into the flask through the cylinder, and block in the mouth of the culture flask through the buckle, realize fixing the cylinder, stretch into the cylinder directly at last and absorb the supernatant fluid that the screen cloth mechanism filters out with the pipettor, the overall structure is simple, easy to operate, can be convenient at any time and take out the clarified supernatant fluid from the flask; and utilize screen cloth mechanism to filter, can effectual assurance supernatant's quality.

In a further embodiment, the screen mechanism comprises a snap ring and a screen body, a filter cavity matched with the screen body is arranged at the bottom end of the barrel and is communicated with the barrel through a through hole, the screen body is placed in the snap ring of the filter cavity, the snap ring is of a circular ring structure, the snap ring is sleeved in the filter cavity, the snap ring is in threaded connection with the filter cavity, the screen body is directly placed in the filter cavity, the snap ring is arranged in the filter cavity, and then the screen body is used for filtering operation; the whole mounting structure is very convenient to disassemble and assemble and is convenient to clean or replace the screen body, so that the filtering quality of the screen body is always ensured.

A protective net is arranged at the bottom of the inner cavity of the cylinder body, and the protective net is used for preventing the pipettor from touching the screen body, so that the service life of the screen body is ensured; the inner circular surface of snap ring is even is equipped with a plurality of anti-skidding archs to improve the dismouting convenience of snap ring, be convenient for later stage installation or pull down the snap ring.

In a further embodiment, the screen cloth body includes upper cover body and lower cover body, upper cover body and lower cover body all are the ring structure, the inner chamber diapire of lower cover body is equipped with the cardboard, the cardboard is the ring board, is equipped with the draw-in groove on the inner chamber wall of cardboard, install the filter layer in the draw-in groove, cell filtration PC membrane or disposable cell screen cloth or stainless steel cell sieve are selected for use to the filter layer, the even through connection of the roof of draw-in groove has a plurality of card holes, the inner chamber roof fixed mounting of upper cover body has a plurality of bayonet locks with card hole matched with, through putting into the draw-in groove the filter layer, then upper cover body folds with lower cover body, utilizes the bayonet lock to insert in the bayonet lock to the firm card of filter layer in the draw-in groove, and whole structure is simple to.

The inner chamber roof of lid is even down is equipped with a plurality of solid fixed cylinders, the inner chamber of going up the lid is even be equipped with a plurality of and solid fixed cylinder matched with bolt, utilizes mutually supporting of bolt and solid fixed cylinder, can be in the same place the firm combination of lid and lower lid, guarantees the installation of filter layer stably, guarantees filtration quality.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model feeds the screen mechanism into the culture bottle through the cylinder body, and is clamped at the culture bottle opening through the buckle, so as to realize the fixation of the cylinder body, and finally, the pipettor directly extends into the cylinder body to absorb the supernatant filtered by the screen mechanism, so that the whole structure is simple, the operation is convenient, and the clarified supernatant can be conveniently taken out from the culture bottle at any time; the quality of the supernatant can be effectively ensured by filtering by using a screen mechanism;

2. the utility model is characterized in that the screen body is directly placed into the filtering cavity, then the snap ring is arranged in the filtering cavity, and then the screen body is utilized to carry out filtering operation; the whole mounting structure is very convenient to disassemble and assemble, and the screen body is convenient to clean or replace, so that the filtering quality of the screen body is always ensured;

3. the utility model discloses an in putting into the draw-in groove to the filter layer, then go up the lid and fold with lower lid, it is downthehole to utilize the bayonet lock to insert the card to in the draw-in groove, whole structural installation is simple, is convenient for change the filter layer.

Drawings

FIG. 1 is a schematic diagram of a perspective view of a filter membrane lid capable of obtaining a clarified cell supernatant;

FIG. 2 is a schematic structural diagram showing a relationship between a screen mechanism of a filter cover and a cylinder for obtaining supernatant of clarified cells;

FIG. 3 is a schematic diagram of a screen body of a filter cover capable of obtaining clarified cell supernatant.

In the figure: 1-screen mesh mechanism, 101-snap ring, 102-anti-skid protrusion, 2-cylinder, 21-filter cavity, 22-through hole, 23-protective screen, 3-buckle, 4-screen mesh body, 41-upper cover body, 42-lower cover body, 43-fixed cylinder, 44-bolt, 45-snap plate, 46-snap groove, 47-filter layer, 48-snap hole and 49-snap pin.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1

Referring to fig. 1, a filter membrane cover capable of obtaining clarified cell supernatant comprises a screen mechanism 1 and a cylinder 2, wherein the cylinder 2 is a circular tube structure, a plurality of buckles 3 are uniformly arranged at the top end of the outer circular surface of the cylinder 2, the bottom end of the cylinder 2 is communicated with the screen mechanism 1, the length of the cylinder 2 is not less than 10cm, the cylinder body 2 is made of original polystyrene material or stainless steel material, when the culture cells in the culture bottle are mature and the supernatant needs to be collected, under the sterile environment, the cover of the culture bottle is opened, then the screen mechanism 1 is sent into the culture bottle through the cylinder body 2, the culture bottle is clamped at the mouth of the culture bottle through a buckle 3 to fix the cylinder body 2, and finally a liquid transfer device is directly inserted into the cylinder body 2 to absorb the supernatant filtered by the screen mechanism 1, so that the whole structure is simple, the operation is convenient, and the clarified supernatant can be conveniently taken out from the culture bottle at any time; and utilize screen cloth mechanism 1 to filter, can effectual assurance supernatant's quality.

Example 2

Referring to fig. 2, the difference from example 1 is: the screen mechanism 1 comprises a clamping ring 101 and a screen body 4, a filter cavity 21 matched with the screen body 4 is arranged at the bottom end of the cylinder body 2, the filter cavity 21 is communicated with the cylinder body 2 through a through hole 22, the screen body 4 is placed in the clamping ring 101 of the filter cavity 21, the clamping ring 101 is of a circular ring structure, the clamping ring 101 is sleeved in the filter cavity 21, the clamping ring 101 is in threaded connection with the filter cavity 21, the screen body 4 is directly placed in the filter cavity 21, the clamping ring 101 is arranged in the filter cavity, and then the screen body 4 is used for filtering operation; the whole mounting structure is very convenient to disassemble and assemble, and is convenient for cleaning or replacing the screen body 4, so that the filtering quality of the screen body 4 is always ensured.

A protective net 23 is arranged at the bottom of the inner cavity of the barrel 2, and the protective net 23 is used for preventing the pipette from touching the screen body 4, so that the service life of the screen body 4 is ensured; the inner circular surface of the snap ring 101 is uniformly provided with a plurality of anti-skidding protrusions 102, so that the disassembly and assembly convenience of the snap ring 101 is improved, and the snap ring 101 can be conveniently installed or disassembled in the later period.

Example 3

Referring to fig. 3, the difference from example 2 is: the screen body 4 comprises an upper cover body 41 and a lower cover body 42, the upper cover body 41 and the lower cover body 42 are both of a circular ring structure, a clamping plate 45 is arranged on the bottom wall of an inner cavity of the lower cover body 42, the clamping plate 45 is a circular ring plate, a clamping groove 46 is formed in the wall of the inner cavity of the clamping plate 45, a filtering layer 47 is installed in the clamping groove 46, the filtering layer 47 is a cell filtering PC membrane or a disposable cell screen or a stainless steel cell screen, a plurality of clamping holes 48 are uniformly connected to the top wall of the clamping groove 46 in a penetrating manner, a plurality of clamping pins 49 matched with the clamping holes 48 are fixedly installed on the top wall of the inner cavity of the upper cover body 41, the filtering layer 47 is placed into the clamping groove 46, then the upper cover body 41 and the lower cover body 42 are folded, the clamping pins 49 are inserted into the clamping holes 48, and the filtering layer 47 is.

The even a plurality of solid fixed cylinder 43 that are equipped with of inner chamber roof of lower lid 42, the even a plurality of bolts 44 with solid fixed cylinder 43 matched with that are equipped with of inner chamber of last lid 41 utilizes mutually supporting of bolt 44 and solid fixed cylinder 43, can be in the same place upper lid 41 and the firm combination of lower lid 42, guarantees that the installation of filter layer 47 is stable, guarantees filtration quality.

Working principle of examples 1 to 3: treat that the blake bottle culture cell is ripe after, when needing to collect the supernatant, under sterile environment, open the blake bottle lid, then send into the blake bottle through barrel 2 screen cloth mechanism 1 to block in the cultivation bottleneck through buckle 3, realize fixing barrel 2, directly stretch into the supernatant that screen cloth mechanism 1 filtered out in barrel 2 with the pipettor at last.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (7)

1. The utility model provides a can acquire filtering membrane lid of clarification cell supernatant, includes screen cloth mechanism (1) and barrel (2), its characterized in that, barrel (2) are round tubular structure, and barrel (2) outer disc top is even is equipped with a plurality of buckles (3), the bottom through connection screen cloth mechanism (1) of barrel (2), and the length of barrel (2) is not less than 10cm, native polystyrene material or stainless steel material are chooseed for use in barrel (2).

2. The membrane cover capable of obtaining the supernatant of the clarified cells as claimed in claim 1, wherein the screen mechanism (1) comprises a snap ring (101) and a screen body (4), the bottom end of the cylinder (2) is provided with a filter chamber (21) matched with the screen body (4), the filter chamber (21) is connected with the cylinder (2) through a through hole (22), the screen body (4) is placed in the snap ring (101) of the filter chamber (21), the snap ring (101) is of a circular ring structure, the snap ring (101) is sleeved in the filter chamber (21), and the snap ring (101) is in threaded connection with the filter chamber (21).

3. The filter membrane cover capable of obtaining clarified cell supernatant as claimed in claim 2, wherein a protective screen (23) is installed at the bottom of the inner cavity of the cylinder (2).

4. The membrane cover capable of obtaining clarified cell supernatant according to claim 2, wherein the inner circumferential surface of the snap ring (101) is uniformly provided with a plurality of anti-slip protrusions (102).

5. The filter membrane cover capable of obtaining the clarified cell supernatant as claimed in claim 2, wherein the filter membrane body (4) comprises an upper cover body (41) and a lower cover body (42), both the upper cover body (41) and the lower cover body (42) are of a circular ring structure, a clamping plate (45) is arranged on the bottom wall of an inner cavity of the lower cover body (42), the clamping plate (45) is a circular ring plate, a clamping groove (46) is formed in the wall of the inner cavity of the clamping plate (45), a filter layer (47) is installed in the clamping groove (46), a plurality of clamping holes (48) are uniformly connected to the top wall of the clamping groove (46) in a penetrating manner, and a plurality of clamping pins (49) matched with the clamping holes (48) are fixedly installed on the top wall of the inner cavity of the upper cover body (.

6. A filter membrane cover capable of obtaining clarified cell supernatant according to claim 5, wherein the filter layer (47) is selected from a cell filtration PC membrane or a disposable cell screen or a stainless steel cell screen.

7. The membrane cover according to claim 5, wherein the top wall of the inner cavity of the lower cover body (42) is uniformly provided with a plurality of fixed cylinders (43), and the inner cavity of the upper cover body (41) is uniformly provided with a plurality of pins (44) matched with the fixed cylinders (43).

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