CN214472777U

CN214472777U - Micro-droplet chip analyzer with linearly arranged LED light sources

Info

Publication number: CN214472777U
Application number: CN202120526698.6U
Authority: CN
Inventors: 白宇; 韩应敏; 刘金伟; 夏雷
Original assignee: Xinyi Manufacturing Technology Beijing Co ltd
Current assignee: Xinyi Manufacturing Technology Beijing Co ltd
Priority date: 2021-03-14
Filing date: 2021-03-14
Publication date: 2021-10-22
Anticipated expiration: 2031-03-14

Abstract

The utility model provides a micro-droplet chip analyzer who contains linear arrangement LED light source, including first many light path subassemblies, the many light path subassemblies of second, include a plurality of LED light source subassemblies in first many light path subassemblies and the many light path subassemblies of second respectively, LED light source subassembly is used for sending the exciting light of predetermineeing the wavelength, the wavelength of predetermineeing of the exciting light that a plurality of LED light source subassemblies in the first many light path subassemblies sent respectively with the wavelength of predetermineeing of the exciting light that a plurality of LED light source subassemblies in the many light path subassemblies of second sent respectively is different and the interval is adjoined each other. The utility model discloses in the wavelength of predetermineeing of the exciting light that a plurality of LED light source subassemblies that first many light path subassemblies and second many light path subassemblies have respectively sent different and interval are adjoined each other, can make each of the exciting light that sends in the same many light path subassemblies predetermine the difference between the wavelength great, the adjacent interference problem of predetermineeing between the wavelength signal of solution that can be better, showing the single detection index that has increased the detection liquid, improved detection efficiency.

Description

Micro-droplet chip analyzer with linearly arranged LED light sources

Technical Field

The utility model belongs to the technical field of digital PCR analysis appearance, concretely relates to little liquid drop chip analysis appearance that contains linear arrangement LED light source.

Background

Digital PCR is a recent quantitative technique, which is an absolute quantitative method for nucleic acid quantification based on a single-molecule PCR method for counting. The method mainly adopts a micro-fluidic or micro-droplet method to disperse a large amount of diluted nucleic acid solution into micro-reactors or micro-droplets of a chip, wherein the number of nucleic acid templates in each reactor is less than or equal to 1. Thus, after PCR cycling, the microdroplets are illuminated with light of a particular wavelength, a reactor with a nucleic acid molecule template will give a particular fluorescent signal, and a reactor without a template will not. Based on the relative proportions and the volume of the reactor, the nucleic acid concentration of the original solution can be deduced. In the micro-droplet chip analyzer, the optical path device is an important device for irradiating the micro-droplets with excitation light of a specific wavelength and causing the fluorescent groups in the micro-droplets to emit fluorescence of the specific wavelength. Because fluorescence signals with adjacent wavelengths are easy to interfere with each other, and the signal analysis precision is affected, most of the traditional micro droplet chip analyzers adopt 2-optical path devices and 3-optical path devices. With the development of science and technology, higher requirements are put forward for the detection speed, efficiency, flux and the like of the micro droplet chip analyzer in medical institutions, scientific research institutions and the like, and new challenges are put forward for the optical path device of the micro droplet chip analyzer.

SUMMERY OF THE UTILITY MODEL

Therefore, the to-be-solved technical problem of the utility model is to provide a little liquid drop chip analysis appearance that contains linear arrangement LED light source, the wavelength of predetermineeing of the exciting light that a plurality of LED light source subassemblies that first many light path subassemblies and second many light path subassemblies have respectively sent is different and the interval is adjoined to each other, and the adjacent interference problem of predetermineeing between the wavelength signal of solution that can be better is showing the single detection index that has increased the detection liquid, has improved detection efficiency.

In order to solve the above problem, the utility model provides a little liquid drop chip analysis appearance that contains linear arrangement LED light source, including first many light path subassemblies, second many light path subassemblies and light path supporting component, include a plurality of LED light source subassemblies in first many light path subassemblies and the second many light path subassemblies respectively, LED light source subassembly includes linear arrangement's LED light source in proper order, LED light source subassembly is used for sending the exciting light of predetermineeing the wavelength, the predetermined wavelength of the exciting light that a plurality of LED light source subassemblies in the first many light path subassemblies sent respectively with the predetermined wavelength of the exciting light that a plurality of LED light source subassemblies in the second many light path subassemblies sent respectively is different each other and the interval borders on.

Preferably, the optical path supporting component comprises a first optical path supporting plate and a second optical path supporting plate which are arranged at an interval relatively, a first optical path connecting plate and a second optical path connecting plate are connected between the first optical path supporting plate and the second optical path supporting plate at an interval relatively, the first optical path connecting plate is connected with the first multi-optical-path component, the second optical path connecting plate is connected with the second multi-optical-path component, and a space between the first optical path connecting plate and the second optical path connecting plate is used for placing the micro-droplet chip.

Preferably, the second optical path connecting plate is slidably connected between the first optical path supporting plate and the second optical path supporting plate along a first direction, and after the position adjustment of the second multi-optical-path component is completed, the position of the second optical path connecting plate can be locked.

Preferably, one side of the first light path connecting plate, which deviates from the second light path connecting plate, is connected with a screw motor, the first multi-light-path assembly is connected with the first light path connecting plate through the screw motor, and the screw motor can adjust the height of the first multi-light-path assembly.

Preferably, the screw rod motor is connected to the first light path connecting plate through a motor connecting piece, and the motor connecting piece can drive the screw rod motor and the first multi-light path assembly to slide along a second direction relative to the first light path connecting plate, wherein the second direction is different from the first direction in surface and is orthogonal to the first direction.

Preferably, the first optical path connecting plate is further connected to a displacement adjusting assembly, and the displacement adjusting assembly is configured to adjust a position of the first multi-optical-path assembly in the second direction.

Preferably, the displacement adjusting assembly comprises a fine adjustment platform, a first positioning plate and a second positioning plate, the fine adjustment platform is sequentially connected with the first positioning plate and the second positioning plate towards one side of the motor connecting piece, one side of the second positioning plate, which is far away from the first positioning plate, is connected with the motor connecting piece, and when the fine adjustment platform runs, the first positioning plate and the second positioning plate can force the motor connecting piece to slide along the second direction; and/or guide pieces are arranged on two opposite sides of the motor connecting piece and connected with the first light path connecting plate, and the two opposite guide pieces form a sliding guide channel of the motor connecting piece.

Preferably, the axis of the objective lens included in the first multi-light-path assembly and the axis of the objective lens included in the second multi-light-path assembly are parallel to each other and spaced apart by a predetermined distance while being coplanar with the first direction.

Preferably, the preset distance is d, and d is more than or equal to 50 mu m and less than or equal to 200 mu m.

Preferably, the LED light source assembly further includes an LED fixing member, a ball lens, a second plano-convex lens, a light filter, and a lens holder, wherein the LED fixing member, the ball lens, the second plano-convex lens, and the light filter are located in the mounting hole of the lens holder, and the inner side surface of the LED light source is connected to the outer side surface of the lens holder.

The utility model provides a pair of micro-droplet chip analyzer, the wavelength of predetermineeing of the exciting light that a plurality of LED light source subassemblies that first many light path subassemblies and second many light path subassemblies have respectively sent is different and the interval is adjoined each other, can make each of the exciting light that sends in the same many light path subassemblies predetermine the difference between the wavelength great, the adjacent interference problem of predetermineeing between the wavelength signal of solution that can be better, showing the single detection index that has increased the detection liquid, improved detection efficiency.

Drawings

Fig. 1 is a schematic structural diagram of a micro droplet chip analyzer according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of the three-beam optical assembly of FIG. 1;

FIG. 3 is a schematic structural diagram (disassembled) of the LED light source assembly in FIG. 1;

FIG. 4 is a schematic structural diagram of the optical path support assembly of FIG. 1;

FIG. 5 is a schematic view of the multi-optical-path module (three-optical-path module) of FIG. 1 (arrows indicate light transmission paths);

fig. 6 shows excitation spectra and emission spectra of four conventional fluorescent dyes, where the ordinate T% is the transmittance, dimensionless, and represents the percentage of the light flux transmitted through a transparent or translucent body to the incident light flux, and the abscissa is the wavelength.

The reference numerals are represented as:

300. an LED light source assembly; 301. a first LED light source assembly; 302. a second LED light source assembly; 303. a third LED light source assembly; 100. a first multi-light-path assembly; 200. a second multi-light path assembly; 400. an optical path support assembly; 1. an LED light source; 2. an LED fixture; 3. a ball lens; 4. a second plano-convex lens; 5. an optical filter; 6. a lens holder; 7. a first dichroic mirror; 8. a second dichroic mirror; 9. a first mounting member; 10. a second mount; 11. a third mount; 12. an objective lens; 13. a fourth mount; 14. a third dichroic mirror; 15. a fifth mount; 16. a first plano-convex lens; 17. a two-phase color mirror gland bush; 18. a fourth dichroic mirror; 19. an optical fiber connector; 20. a lens connecting member; 21. a first optical path support plate; 22. a second optical path support plate; 23. a second optical path connecting plate; 24. a first optical path connecting plate; 25. fine tuning the platform; 26. a first positioning plate; 27. a second positioning plate; 28. a motor connector; 29. a guide member; 30. a screw motor; 31. micro-droplet chips.

Detailed Description

Referring to fig. 1 to 6 in combination, according to an embodiment of the present invention, a droplet chip analyzer including linear arrangement LED light sources is provided, including a first multi-light-path assembly 100 and a second multi-light-path assembly 200, the first multi-light-path assembly 100 and the second multi-light-path assembly 200 respectively include a plurality of LED light source assemblies 300 therein, the LED light source assemblies 300 are used for emitting excitation light with preset wavelengths, the preset wavelengths of the excitation light respectively emitted by the plurality of LED light source assemblies 300 in the first multi-light-path assembly 100 (the preset wavelengths are selected according to specific detection targets) are different from the preset wavelengths of the excitation light respectively emitted by the plurality of LED light source assemblies 300 in the second multi-light-path assembly 200 and are adjacent at intervals. In the technical scheme, the preset wavelengths of the excitation lights respectively emitted by the LED light source assemblies 300 respectively arranged on the first multi-light-path assembly 100 and the second multi-light-path assembly 200 are different from each other and are adjacent at intervals, and the excitation lights with adjacent wavelengths (excitation lights and emission light wavelengths) can be respectively arranged in the two light-path assemblies, so that the two light paths can be ensured not to be detected on the same liquid drop at any moment, the difference between the preset wavelengths of the excitation lights emitted in the same multi-light-path assembly is large, the interference problem between adjacent preset wavelength signals can be better solved, the single detection index of the detection liquid is obviously increased, the most efficient wave band is adopted for exciting each fluorescent dye to obtain the strongest signal value, and the detection efficiency is improved.

Taking the first multi-light-path assembly 100 and the second multi-light-path assembly 200 as three light-path assemblies as an example, each of the three light-path assemblies includes three LED light source assemblies 300, at this time, when the first multi-light-path assembly 100 and the second multi-light-path assembly 200 are simultaneously used, a six-light-path device of the analyzer is formed at this time, the six LED light source assemblies 300 respectively emit laser light with corresponding preset wavelengths, that is, excitation light with six wavelengths, and the six excitation light wavelengths are respectively set as a (corresponding to the fluorochrome a), B (corresponding to the fluorochrome B), C (corresponding to the fluorochrome C), D (corresponding to the fluorochrome D), E (corresponding to the fluorochrome E) and F (corresponding to the fluorochrome F) according to the serial numbers from small to large, the wavelengths of the laser light are set according to the selection of the fluorochromes, and according to actual conditions, the excitation wavelengths of the selected six fluorochromes are not distributed in an arithmetic progression from 480nm to 700nm, some fluorescent dyes have excitation wavelengths very close to each other (such as fluorescent dyes three and four in fig. 6), some fluorescent dyes have relatively large difference in excitation wavelengths (such as fluorescent dyes one and two in fig. 6), if a six-optical-path device is made into a whole, six excitation lights excite six fluorescent dyes simultaneously, there are a laser one (excitation light wavelength a) to excite dye a, a laser two (excitation light wavelength b) also has a certain excitation effect on dye a, and dye C only has an excitation effect on laser three (excitation light wavelength C), so that the emitted fluorescent signals are inconsistent in strength, the signals need to be split by two phases in the subsequent transmission analysis process, while the existing dichroic mirror has limited technology, cannot effectively split a plurality of lights with close wavelengths, so that the light intensity of a certain light is reduced, or two lights enter the same photomultiplier, therefore, the problem of adjacent optical signal interference is caused, and finally obtained signals cannot reflect actual conditions, and the utility model discloses in be first multi-optical-path component 100 and second multi-optical-path component 200 respectively with a whole six optical-path device, take first multi-optical-path component 100 as an example, the laser serial number that first multi-optical-path component 100 optical path set up is one, three, five, the target fluorochrome serial number that will excite is A, C, E, in subsequent signal analysis, only need to filter the light of the fluorochrome B, D, F that probably exists can, correspondingly, second multi-optical-path component 200 optical path set up laser serial number is B, D, F, signal analysis is the same as first multi-optical-path component 100 optical path, like this, the six optical-path form of each three optical paths that set up respectively is for the form that six optical paths are integrative, the signal interference problem has been solved well.

In one embodiment, the micro droplet chip analyzer further includes an optical path supporting assembly 400, the optical path supporting assembly 400 includes a first optical path supporting plate 21 and a second optical path supporting plate 22 disposed at an interval, a first optical path connecting plate 24 and a second optical path connecting plate 23 are connected between the first optical path supporting plate 21 and the second optical path supporting plate 22 at an interval, the first optical path connecting plate 24 is connected to the first multi-optical-path assembly 100, the second optical path connecting plate 23 is connected to the second multi-optical-path assembly 200, and a space between the first optical path connecting plate 24 and the second optical path connecting plate 23 is used for placing the micro droplet chip 31. In this embodiment, the first multi-optical-path module 100 and the second multi-optical-path module 200 are respectively disposed on the first optical-path connecting plate 24 and the second optical-path connecting plate 23 in an opposing manner, so that the two multi-optical-path modules can be arranged more compactly, and in a specific implementation, the first multi-optical-path module 100 and the second multi-optical-path module 200 are disposed in an opposing manner, and at this time, the droplet chips 31 are placed in a space therebetween by a robot or manually.

Preferably, the second optical path connecting plate 23 is slidably connected between the first optical path supporting plate 21 and the second optical path supporting plate 22 along a first direction, and after the position adjustment of the second multi-optical-path component 200 is completed, the position of the second optical path connecting plate 23 can be locked, the second optical path connecting plate 23 is designed to be slidably connected along the first direction, so that the machining dimension error caused by the fixed connection manner adopted by the second optical path connecting plate 23 can be effectively avoided, and the position of the second optical path connecting plate is more accurate. The aforementioned first direction may specifically be a direction (inward or outward from the paper) which is horizontal and perpendicular to the paper in the use orientation as shown in fig. 1.

Further, one side of the first optical path connecting plate 24 departing from the second optical path connecting plate 23 is connected to a lead screw motor 30, the first multi-optical-path component 100 is connected to the first optical path connecting plate 24 through the lead screw motor 30, and the lead screw motor 30 can adjust the height of the first multi-optical-path component 100, specifically, the first multi-optical-path component 100 is fixedly connected to a mover of the lead screw motor 30, and when the lead screw motor 30 operates, the mover drives the first multi-optical-path component 100 to perform a reciprocating motion (for example, to be raised or lowered, specifically according to the rotating direction of the lead screw motor 30).

Further, the screw rod motor 30 is connected to the first optical path connecting plate 24 through the motor connecting piece 28, and the motor connecting piece 28 can drive the screw rod motor 30 and the first multi-optical-path assembly 100 is opposite to the first optical path connecting plate 24 slides along the second direction, the second direction is different from the first direction and orthogonal, at this time, the height and the horizontal position of the first multi-optical-path assembly 100 can be flexibly adjusted, and the second direction is different from the first direction and orthogonal, so that the height adjustment effect of the screw rod motor 30 is compounded, and the focusing adjustment of the objective lenses 12 in the two optical-path assemblies 100 is more accurate and convenient. It can be understood that, a displacement adjusting assembly is further connected to the first optical path connecting plate 24, the displacement adjusting assembly is used for adjusting the position of the first multi-optical-path assembly 100 in the second direction, specifically, the displacement adjusting assembly includes a fine tuning platform 25, a first positioning plate 26, and a second positioning plate 27, the fine tuning platform 25 is connected to the first positioning plate 26 and the second positioning plate 27 in sequence towards one side of the motor connecting member 28, one side of the second positioning plate 27 away from the first positioning plate 26 is connected to the motor connecting member 28, and when the fine tuning platform 25 operates, the first positioning plate 26 and the second positioning plate 27 can force the motor connecting member 28 to slide in the second direction; and/or, the opposite two sides of the motor connector 28 are provided with guide members 29, the guide members 29 are connected with the first light path connecting plate 24, and the two opposite guide members 29 form a sliding guide channel of the motor connector 28. When the horizontal position of the objective lens 12 (i.e., the first direction and the second direction) is determined, the position of the motor connector 28 relative to the first optical path connecting plate 24 is locked, and then the displacement adjusting assembly may be removed.

In some embodiments, the axis of the objective lens 12 of the first multi-optical-path assembly 100 and the axis of the objective lens 12 of the second multi-optical-path assembly 200 are parallel to each other and separated by a predetermined distance, and are coplanar with the first direction, and the predetermined distance is preferably between 50 μm and 200 μm, as verified by the utility model, in which the signal intensity contrast is significant and the signal anti-interference capability is more significant.

Preferably, the first multi-light-path assembly 100 and the second multi-light-path assembly 200 have the same structure, and it is understood that the other structures are the same except for the LED light source assemblies 300 respectively provided therein. The specific structure of the first multi-optical-path component 100 is described below as a three-optical-path component: the first multi-light-path component 100 includes a first LED light source component 301, a second LED light source component 302, a third LED light source component 303, a first dichroic mirror 7, a second dichroic mirror 8, a third dichroic mirror 14, an objective lens 12, a first plano-convex lens 16, and a fourth dichroic mirror 18, excitation lights respectively emitted by the first LED light source component 301 and the second LED light source component 302 pass through the first dichroic mirror 7, the second dichroic mirror 8, and the third dichroic mirror 14 in sequence, the excitation light emitted by the third LED light source component 303 passes through the second dichroic mirror 8 and the third dichroic mirror 14 in sequence, and then is emitted from the objective lens 12 to micro droplets in channels of the micro droplet chips 31, and fluorescence generated by excitation passes through the objective lens 12, the third dichroic mirror 14, and the first plano-convex lens 16 in sequence, and then enters the optical fiber connector 19 and/or the lens connector 20 at the fourth dichroic mirror 18, it can be understood that the optical fiber connector 19 is connected to an optical fiber to transmit a corresponding fluorescent signal to an electronic component such as a photomultiplier tube, etc. to convert the optical signal into an electrical signal for signal analysis, and the fluorescent signal enters the camera through the lens connector 20, and the camera is connected to a display screen of an industrial personal computer, so that the movement of the micro-droplets in the micro-droplet chip 31 can be displayed and recorded in real time. .

Further, the first multi-light-path assembly 100 comprises a structural body, the structural body comprises a first mounting piece 9, a second mounting piece 10, a third mounting piece 11, a fourth mounting piece 13 and a fifth mounting piece 15, the first mounting piece 9, the second mounting piece 10, the third mounting piece 11, the fourth mounting piece 13 and the fifth mounting piece 15 are assembled into a whole, a light path channel is formed inside the first mounting piece 9, the first dichroic mirror 7 is clamped at the joint of the first mounting piece 9 and the second mounting piece 10, and the second dichroic mirror 8 is clamped at the joint of the first mounting piece 9 and the third mounting piece 11; the objective lens 12 is connected to the mounting hole of the third mounting part 11; the third dichroic mirror 14 is interposed at a connection part of the third mounting member 11 and the fourth mounting member 13; the first plano-convex lens 16 is clamped at the joint of the fourth mounting piece 13 and the fifth mounting piece 15; the fourth dichromatic mirror 18 is clamped between the dichromatic mirror gland 17 and the fifth mounting part 15; the optical fiber connector 19 is connected to an end of the fifth mounting member 15, and the lens connector 20 is connected to a side of the fifth mounting member 15.

The LED light source assembly 300 includes an LED light source 1, an LED fixing member 2, a spherical lens 3, a second plano-convex lens 4, an optical filter 5, and a lens holder 6, which are linearly arranged in sequence, wherein the LED fixing member 2, the spherical lens 3, the second plano-convex lens 4, and the optical filter 5 are located in a mounting hole of the lens holder 6, and an inner side surface of the LED light source 1 is connected with an outer side surface of the lens holder 6.

The utility model discloses a micro-droplet chip analyzer need carry out necessary position determination operation when the assembly debugging, specifically can adopt following mode to go on:

referring to fig. 1 and 4 in combination, when the position is determined during initial installation, the six LED light sources 1 are powered on to emit light with 6 different wavelengths; the screw motor 30 acts to lift the first three optical path assembly (i.e. a specific implementation form of the first multi-optical path assembly 100) to the highest position, so as to make room for placing the micro-droplet chip 31; the micro-droplet chip 31 is placed on a specific structure between the first and second three optical path components (i.e. a specific implementation form of the second multi-optical path component 200) by a manipulator or manually, the specific structure drives the chip to slightly move left and right, up and down (the orientation shown in fig. 1) under algorithm control, so that the micro-droplet in the trench is just at the focus of the objective lens 12 in the second three optical path component, at this time, the micro-droplet can form a clear image on the display screen of the industrial computer, then the lead screw motor 30 acts to lower the first three optical path component to a certain position, the first three optical path component is finally driven to slightly move by the fine adjustment platform 25 on the manual fine adjustment optical path support component 400, the first positioning plate 26, the second positioning plate 27, the motor connecting piece 28 and the lead screw motor 29, and the imaging of the camera on the display industrial computer screen on the first three optical path component is observed, the light emitted by the objective lens on the first three-optical-path component is aligned to the groove on the micro-droplet chip 31, then the bolt on the motor connecting piece 28 is fixed, the position of the first three-optical-path component relative to the optical-path supporting component 400 is fixed through the motor connecting piece 28 and the screw rod motor 30, so that the axes of the upper and lower two objective lenses 12 are positioned on the same vertical plane as shown in figure 1, the fine-adjustment platform 25, the first positioning plate 26, the second positioning plate 27 and the guide piece 29 are taken as assemblies for assembly and can be removed, the front and back positions of the second three-optical-path component are fine-adjusted through manually fine-adjusting the first optical-path connecting plate 23, so that the axes of the upper and lower two objective lenses 12 are positioned on two different planes which are parallel to the paper surface and have the distance of 50-200 mu m, the bolt on the first optical-path connecting plate 23 is fastened, the position of the second three-optical-path component is fixed and does not change, and thus far, the relative positions (horizontal relative positions) of the first three-optical-path component and the second optical-path component in the analyzer are determined The height mode is entirely dependent on the up and down adjustment of the lead screw motor 30 (and it will be appreciated that the detection action after the analyzer no longer requires the position determination process described above). Then, subsequent detection operation can be performed, specifically, the screw motor 30 continues to perform fine movement up and down, so that the micro droplets in the channel are exactly positioned at the focus of the objective lens 12 in the first three optical path components, the controller of the screw motor 30 remembers the position at this time, which can be recorded as the focus position of the first three optical path components, and is used as the position of subsequent repeated movement, the droplets in the channel of the micro droplet chip 31 move from far to near (parallel to the first direction) in the direction perpendicular to the paper surface, and sequentially pass through the irradiation of the light rays emitted by the first three optical path components and the second three optical path components, i.e., the droplets are respectively irradiated by the light rays with 3 different wavelengths at two positions, and are respectively excited to generate fluorescence with 3 different wavelengths, 2 groups of excited fluorescence respectively pass through the respective three optical path components, and finally enter optical devices such as 2 groups of photomultiplier tubes along the optical fiber to be converted into electric signals, and are analyzed through an algorithm, fitting the 2 groups of the 3 optical path fluorescent signal analysis results, and finally outputting a 6 optical path fluorescent signal analysis result. After the droplet analysis is completed, the lead screw motor 30 is operated to lift the first third optical path component to the highest position, and the chip 31 is taken out by a manipulator or manually and placed in a designated recovery device.

It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.

The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims

1. The micro-droplet chip analyzer with the linearly arranged LED light sources is characterized by comprising a first multi-light-path assembly (100), a second multi-light-path assembly (200) and a light path supporting assembly (400), wherein the first multi-light-path assembly (100) and the second multi-light-path assembly (200) respectively comprise a plurality of LED light source assemblies (300), each LED light source assembly (300) comprises the LED light sources (1) which are sequentially and linearly arranged, each LED light source assembly (300) is used for emitting exciting light with preset wavelength, and the preset wavelength of the exciting light respectively emitted by the LED light source assemblies (300) in the first multi-light-path assembly (100) and the preset wavelength of the exciting light respectively emitted by the LED light source assemblies (300) in the second multi-light-path assembly (200) are different from each other and are adjacent at intervals.

2. The micro droplet chip analyzer according to claim 1, wherein the optical path support assembly (400) comprises a first optical path support plate (21) and a second optical path support plate (22) which are disposed at an interval, a first optical path connection plate (24) and a second optical path connection plate (23) are connected between the first optical path support plate (21) and the second optical path support plate (22) at an interval, the first optical path connection plate (24) is connected with the first multi-optical path assembly (100), the second optical path connection plate (23) is connected with the second multi-optical path assembly (200), and a space between the first optical path connection plate (24) and the second optical path connection plate (23) is used for placing the micro droplet chip (31).

3. The micro droplet chip analyzer according to claim 2, wherein the second optical path connecting plate (23) is slidably connected between the first optical path supporting plate (21) and the second optical path supporting plate (22) in a first direction, and the position of the second optical path connecting plate (23) can be locked after the position adjustment of the second multi-optical-path component (200) is finished.

4. The micro droplet chip analyzer according to claim 2, wherein a lead screw motor (30) is connected to a side of the first light path connecting plate (24) away from the second light path connecting plate (23), the first multi-light path module (100) is connected to the first light path connecting plate (24) through the lead screw motor (30), and the lead screw motor (30) is capable of adjusting the height of the first multi-light path module (100).

5. The micro droplet chip analyzer according to claim 4, wherein the lead screw motor (30) is connected to the first optical path connecting plate (24) through a motor connector (28), and the motor connector (28) can drive the lead screw motor (30) and the first multi-optical-path component (100) to slide relative to the first optical path connecting plate (24) along a second direction, the second direction being different from and orthogonal to the first direction.

6. The micro droplet chip analyzer according to claim 5, wherein a displacement adjusting member for adjusting the position of the first multi-optical-path component (100) in the second direction is further connected to the first optical-path connecting plate (24).

7. The micro droplet chip analyzer according to claim 6, wherein the displacement adjusting assembly comprises a fine adjustment platform (25), a first positioning plate (26), and a second positioning plate (27), wherein the fine adjustment platform (25) is connected to the first positioning plate (26) and the second positioning plate (27) in sequence on a side facing the motor connecting member (28), and a side of the second positioning plate (27) away from the first positioning plate (26) is connected to the motor connecting member (28), and when the fine adjustment platform (25) operates, the first positioning plate (26) and the second positioning plate (27) can force the motor connecting member (28) to slide along the second direction; and/or guide pieces (29) are arranged on two opposite sides of the motor connecting piece (28), the guide pieces (29) are connected with the first light path connecting plate (24), and the two oppositely arranged guide pieces (29) form a sliding guide channel of the motor connecting piece (28).

8. The micro droplet chip analyzer according to claim 3, wherein the axis of the objective lens (12) provided in the first multi-light-path assembly (100) and the axis of the objective lens (12) provided in the second multi-light-path assembly (200) are parallel to each other and spaced a predetermined distance apart while being coplanar with the first direction.

9. The micro droplet chip analyzer of claim 8, wherein the predetermined distance is d, d is 50 μm or less and 200 μm or less.

10. The micro droplet chip analyzer according to claim 1, wherein the LED light source assembly (300) further comprises an LED holder (2), a ball lens (3), a second plano-convex lens (4), a filter (5), and a lens holder (6), wherein the LED holder (2), the ball lens (3), the second plano-convex lens (4), and the filter (5) are located in a mounting hole of the lens holder (6), and an inner side surface of the LED light source (1) is connected with an outer side surface of the lens holder (6).