CN212302057U - Fluorescent lighting device for stereomicroscope - Google Patents
Fluorescent lighting device for stereomicroscope Download PDFInfo
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- CN212302057U CN212302057U CN202021285938.XU CN202021285938U CN212302057U CN 212302057 U CN212302057 U CN 212302057U CN 202021285938 U CN202021285938 U CN 202021285938U CN 212302057 U CN212302057 U CN 212302057U
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Abstract
The utility model aims at the defects of the prior art, the utility model provides a stereoscopic microscope fluorescence lighting device, which uses a V-shaped double-hole filter box module, a V-shaped oblique 45-degree angle installation surface is arranged, a dichroic mirror is installed on one installation surface, the dichroic mirror is not installed on the other installation surface, an excitation light filter is installed on one side of the dichroic mirror, and the excitation light filter is not needed to be installed on the other side, thereby realizing the single-path lighting in the stereoscopic microscope, after a fluorescence signal emitted to a full space angle passes through the double-hole filter box, one path of the fluorescence signal enters an imaging module after passing through the dichroic mirror and the fluorescence filter, and the other path of the fluorescence signal can enter the imaging module after directly passing through the fluorescence filter, through the method, the excitation light filter and the dichroic mirror are simplified, the system cost and the external dimension are reduced, in addition, through using, a plurality of V-shaped double-hole filter box modules can be installed at the same time, so that the system can simultaneously use a plurality of different excitation and fluorescence wavelength channels to realize fluorescence illumination and imaging of more biological samples.
Description
Technical Field
The utility model belongs to the technical field of the formation of image, concretely relates to stereoscopic microscope fluorescence lighting device.
Background
The stereomicroscope is a scientific research instrument capable of providing stereoscopic vision, and can provide microscopic images for users at a microscopic level. Because the double-channel imaging system is used, binocular stereo vision is provided for a user, and corresponding operation is convenient to perform on a microscopic level, so that the double-channel imaging system is widely applied. However, because there are a large number of biological samples that are relatively transparent and not easily viewed under a microscope, it has been proposed to use fluorescence imaging to image specific components of a biological sample to improve the contrast of the image. However, in the stereomicroscope, because a dual-path imaging system exists at the same time, a dual-path fluorescence illumination module is often required to realize good fluorescence imaging. This undoubtedly increases the system structure and cost.
Aiming at the problems, the fluorescence lighting device of the stereoscopic microscope is provided by the patent by combining the structural characteristics of the stereoscopic microscope and the characteristics of fluorescence excitation and emission, and adopting a system structure of single-path excitation and double-path imaging to simplify the fluorescence lighting module light path of the stereoscopic microscope.
Disclosure of Invention
The utility model aims at prior art not enough, provide a stereoscopic microscope fluorescence lighting device, utilize and to replace light source interface, relay lens installation pipe, multi-wavelength filter box mount pad, V-arrangement diplopore filter box module and wavelength selection slider, realize the fluorescence illumination under the multiple wavelength, on the basis of guaranteeing the physical vision imaging effect, simplified the light path structure to the wavelength compatibility of system has been increased.
The utility model provides a technical scheme that its technical problem adopted as follows:
the utility model comprises a shell, a replaceable light source interface, a relay lens mounting tube, a multi-wavelength light filter box mounting seat, a V-shaped double-hole light filter box module and a wavelength selection slide block, wherein the replaceable light source interface can be connected with a fluorescent lighting source, light emitted by the light source is transmitted in a focusing state after passing through a relay lens in the relay lens mounting tube and enters the V-shaped double-hole light filter box module mounted in the multi-wavelength light filter box mounting seat, an exciting light filter is mounted at a light inlet in the V-shaped double-hole light filter box module and is used for filtering useless wavelengths outside the exciting light wavelength, the V-shaped double-hole light filter box module is provided with two V-shaped mounting surfaces inclined at an angle of 45 degrees, a dichroic mirror is mounted on one V-shaped mounting surface and is used for reflecting the exciting wavelength in the illuminating light and is emitted from a left light hole of the V, the sample emits fluorescence to a full space angle under the action of excitation wavelength in illumination light, part of fluorescence signals reversely transmit through a left light hole in a V-shaped double-hole optical filter box module, further transmit a dichroic mirror and a left fluorescence optical filter, part of the fluorescence signals reversely transmit through a right light hole in the V-shaped double-hole optical filter box module, and further transmit a right fluorescence optical filter, the left fluorescence optical filter and the right fluorescence optical filter have the same optical property and are used for filtering noise light wavelength except the fluorescence wavelength and improving the signal-to-noise ratio of fluorescence imaging, N V-shaped double-hole optical filter box module installation grooves are arranged in a multi-wavelength optical filter box installation seat, N is an integer larger than 1, a guide rail is arranged in the multi-wavelength optical filter box installation seat, and the N V-shaped double-hole optical filter box modules are driven to slide on the guide rail by pushing and pulling a wavelength selection sliding, the selection of the corresponding operating wavelength is realized.
The multi-wavelength optical filter box mounting seat is provided with a mounting seat in the middle, the mounting seat is internally provided with N mounting grooves and 2N threaded holes, N V-shaped double-hole optical filter box modules can be sequentially inserted into the mounting grooves and fixed through the threaded holes on the left side and the right side, two guide rails are arranged on two sides of the mounting seat, the mounting seat can move back and forth on the guide rails, and a proper V-shaped double-hole optical filter box module is selected for fluorescent lighting.
The V-shaped double-hole optical filter box module is characterized in that an external fluorescent lighting source is introduced through a replaceable light source interface, the fluorescent lighting source comprises a light source heat dissipation plate, light source lamp beads and a condensing lens, lighting rays emitted by the light source lamp beads are subjected to beam shaping through the condensing lens and then enter the V-shaped double-hole optical filter box module through a relay lens, an excitation light filter is vertically arranged on the left side of the V-shaped double-hole optical filter box module and is used for filtering useless wavelengths outside the wavelength of the excitation light in the lighting rays, a V-shaped oblique 45-degree angle mounting surface is arranged in the middle of the V-shaped double-hole optical filter box module, a dichroic mirror is arranged on the left side mounting surface, no mirror is arranged on the right side mounting surface, the dichroic mirror on the left side mounting surface reflects the excitation light wavelength in the lighting rays and emits out of a left side light hole, and the part of the fluorescence reversely passes through the right light hole, directly passes through the right mounting surface, further passes through the right fluorescent filter and then enters the imaging module of the microscope.
The utility model has the advantages that:
through the structure arrangement of the utility model, a stereoscopic microscope fluorescence lighting device can be realized, the device uses a V-shaped double-hole filter box module, a V-shaped oblique 45-degree angle installation surface is arranged, a dichroic mirror is installed on one installation surface, the dichroic mirror is not installed on the other installation surface, an excitation light filter is installed on one side of the dichroic mirror, the excitation light filter is not installed on the other side, thereby realizing the single-path lighting in the stereoscopic microscope, after a fluorescence signal emitted to a full space angle passes through the double-hole filter box, one path of the fluorescence signal enters an imaging module after passing through the dichroic mirror and the fluorescence filter, and the other path of the fluorescence signal can also enter the imaging module after directly passing through the fluorescence filter, a plurality of V-shaped double-hole filter box modules can be installed at the same time, so that the system can simultaneously use a plurality of different excitation and fluorescence wavelength channels to realize fluorescence illumination and imaging of more biological samples.
Drawings
Fig. 1 is a schematic view of a stereoscopic microscope fluorescence illumination device.
Fig. 2 is a schematic view of a multi-wavelength filter cartridge mount.
Fig. 3 is a schematic view of a V-shaped dual-aperture filter cartridge module.
Detailed Description
In order to make the public more clearly understand the technical essence and the beneficial effects of the present invention, the applicant shall make the following detailed description by way of examples, but the description of the examples is not a limitation to the solution of the present invention, and any equivalent changes made according to the inventive concept, which are only formal and immaterial, shall be regarded as the technical scope of the present invention.
Example 1
The invention is further described in the following with reference to fig. 1, fig. 2, fig. 3 and embodiment 1.
As shown in the attached figure 1, the utility model comprises a shell 1, a replaceable light source interface 2, a relay lens installation tube 3, a multi-wavelength filter box installation seat 4, a V-shaped double-hole filter box module 5 and a wavelength selection slide block 6, wherein the replaceable light source interface 2 can be connected with a fluorescent lighting light source, light emitted by the light source is transmitted in a focusing state after passing through a relay lens in the relay lens installation tube 3 and enters the V-shaped double-hole filter box module 5 installed in the multi-wavelength filter box installation seat 4, an exciting light filter is installed at a light inlet in the V-shaped double-hole filter box module 5 and is used for filtering useless wavelengths outside the exciting light wavelength, the V-shaped double-hole filter box module 5 is provided with two V-shaped installation surfaces with 45-degree angles, wherein, a dichroic mirror is installed on one V-shaped installation surface and is used for reflecting the exciting wavelength in the lighting light and is emitted from a left light hole of, the multi-wavelength filter cartridge mounting seat is used for illuminating a sample, the sample emits fluorescence to a full space angle under the action of excitation wavelength in illumination light, part of fluorescence signals reversely transmit through a left light hole in a V-shaped double-hole filter cartridge module 5, further transmit a dichroic mirror and a left fluorescence filter, part of the fluorescence signals reversely transmit through a right light hole in the V-shaped double-hole filter cartridge module 5, further transmit a right fluorescence filter, the optical properties of the left fluorescence filter and the right fluorescence filter are the same, the left fluorescence filter and the right fluorescence filter are used for filtering noise light wavelength except the fluorescence wavelength, the signal to noise ratio of fluorescence imaging is improved, 3V-shaped double-hole filter cartridge module mounting grooves can be mounted in the multi-wavelength filter cartridge mounting seat 4, a guide rail is arranged in the multi-wavelength filter cartridge mounting seat, and the 3V-shaped double-hole filter cartridge modules are driven to slide on the, the selection of the corresponding operating wavelength is realized.
As shown in fig. 2, the middle of the multi-wavelength optical filter box mounting base is provided with a mounting base 1, the mounting base 1 is provided with 3 mounting grooves and 6 threaded holes, a V-shaped double-hole optical filter box module 2-1, a V-shaped double-hole optical filter box module 2-2 and a V-shaped double-hole optical filter box module 2-3 can be sequentially inserted into the mounting grooves and fixed through the threaded holes on the left side and the right side, the two sides of the mounting base 1 are provided with a guide rail 3-1 and a guide rail 3-2, the mounting base 1 can move back and forth in the guide rail 3-1 and the guide rail 3-2, and a proper V-shaped double-hole optical filter box module is selected for fluorescent lighting.
As shown in fig. 3, the V-shaped dual-hole filter cartridge module introduces an external fluorescent lighting source through a replaceable light source interface, the fluorescent lighting source comprises a light source heat dissipation plate 1, a light source lamp bead 2 and a condenser lens 3, lighting light emitted by the light source lamp bead 2 is shaped by the condenser lens 3 and then enters the V-shaped dual-hole filter cartridge module through a relay lens 4, an excitation light filter 5 is vertically installed on the left side of the V-shaped dual-hole filter cartridge module and used for filtering useless wavelengths outside the wavelength of excitation light in the lighting light, a V-shaped oblique 45-degree installation surface is arranged in the middle of the V-shaped dual-hole filter cartridge module, a dichroic mirror is installed on the left side installation surface 6, no lens is installed on the right side installation surface 7, the excitation light wavelength in the lighting light is reflected by the dichroic mirror on the left side installation surface 6 and is emitted from, the fluorescence signal is emitted to a full space angle, part of fluorescence reversely passes through the left side light hole 8, penetrates through the dichroic mirror of the left side installation surface 6, further passes through the left side fluorescence optical filter 9, and then enters the imaging module of the microscope, and part of fluorescence reversely passes through the right side light hole 10, directly penetrates through the right side installation surface 7, further passes through the right side fluorescence optical filter 11, and then enters the imaging module of the microscope.
Claims (3)
1. A fluorescence lighting device of a stereomicroscope is characterized by comprising a shell, a replaceable light source interface, a relay lens mounting tube, a multi-wavelength light filter box mounting seat, a V-shaped double-hole light filter box module and a wavelength selection sliding block, wherein the replaceable light source interface can be connected with a fluorescence lighting source, light rays emitted by the light source are transmitted in a focusing state after passing through a relay lens in the relay lens mounting tube and enter the V-shaped double-hole light filter box module mounted in the multi-wavelength light filter box mounting seat, an exciting light filter is mounted at a light inlet in the V-shaped double-hole light filter box module and is used for filtering useless wavelengths outside the exciting light wavelengths, the V-shaped double-hole light filter box module is provided with two V-shaped mounting surfaces inclined at an angle of 45 degrees, a dichroic mirror is mounted on one V-shaped mounting surface and is used for reflecting the exciting wavelength in the lighting light rays and is emitted from a light hole, the multi-wavelength filter cartridge mounting seat is used for illuminating a sample, the sample emits fluorescence to a full space angle under the action of excitation wavelength in illumination light, part of fluorescence signals reversely transmit through a left light hole in a V-shaped double-hole filter cartridge module, further transmit a dichroic mirror and a left fluorescence filter, part of the fluorescence signals reversely transmit through a right light hole in the V-shaped double-hole filter cartridge module, further transmit a right fluorescence filter, the optical properties of the left fluorescence filter and the right fluorescence filter are the same, the left fluorescence filter and the right fluorescence filter are used for filtering noise light wavelengths except the fluorescence wavelengths, the signal to noise ratio of fluorescence imaging is improved, N V-shaped double-hole filter cartridge module mounting grooves are arranged in the multi-wavelength filter cartridge mounting seat, N is an integer larger than 1, a guide rail is arranged in the multi-wavelength filter cartridge push-pull mounting seat, and a slide block is selected through the wavelength to drive the N V-, the selection of the corresponding operating wavelength is realized.
2. The stereomicroscope fluorescence lighting device of claim 1, wherein the multi-wavelength filter cartridge mounting base has a mounting base in the middle, the mounting base has N mounting slots and 2N threaded holes, N V-shaped dual-hole filter cartridge modules can be inserted into the mounting slots in sequence and fixed by the threaded holes on the left and right sides, two guide rails are arranged on both sides of the mounting base, the mounting base can move back and forth on the guide rails, and the appropriate V-shaped dual-hole filter cartridge module is selected for fluorescence lighting.
3. The stereomicroscope fluorescence illumination device of claim 1, wherein the V-shaped dual-hole filter cartridge module is introduced with an external fluorescence illumination light source through a replaceable light source interface, the fluorescence illumination light source comprises a light source heat dissipation plate, a light source lamp bead and a condenser lens, illumination light emitted by the light source lamp bead passes through the condenser lens for beam shaping and then enters the V-shaped dual-hole filter cartridge module through a relay lens, an excitation light filter is vertically installed on the left side of the V-shaped dual-hole filter cartridge module for filtering out useless wavelengths outside the wavelength of the excitation light in the illumination light, a V-shaped 45-degree oblique installation surface is arranged in the middle of the V-shaped dual-hole filter cartridge module, a dichroic mirror is installed on the left side installation surface, no lens is installed on the right side installation surface, and the dichroic mirror on the left side installation surface reflects the excitation light wavelength in the illumination light and emits, the fluorescence imaging device is used for illuminating a sample to emit fluorescence, a fluorescence signal is emitted to a full space angle, partial fluorescence reversely passes through the left light hole, penetrates through the dichroic mirror on the left mounting surface, further passes through the left fluorescence optical filter and then enters the imaging module of the microscope, partial fluorescence reversely passes through the right light hole, directly penetrates through the right mounting surface, and further passes through the right fluorescence optical filter and then also enters the imaging module of the microscope.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021285938.XU CN212302057U (en) | 2020-07-05 | 2020-07-05 | Fluorescent lighting device for stereomicroscope |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021285938.XU CN212302057U (en) | 2020-07-05 | 2020-07-05 | Fluorescent lighting device for stereomicroscope |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112987273A (en) * | 2021-04-15 | 2021-06-18 | 南方科技大学 | Fluorescence excitation block light source and fluorescence microscope |
| CN113504636A (en) * | 2021-08-03 | 2021-10-15 | 嘉兴智瞳科技有限公司 | Imaging system with 3D imaging and fluorescence imaging functions |
-
2020
- 2020-07-05 CN CN202021285938.XU patent/CN212302057U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112987273A (en) * | 2021-04-15 | 2021-06-18 | 南方科技大学 | Fluorescence excitation block light source and fluorescence microscope |
| CN112987273B (en) * | 2021-04-15 | 2023-08-29 | 南方科技大学 | Fluorescence excitation block light source and fluorescence microscope |
| CN113504636A (en) * | 2021-08-03 | 2021-10-15 | 嘉兴智瞳科技有限公司 | Imaging system with 3D imaging and fluorescence imaging functions |
| CN113504636B (en) * | 2021-08-03 | 2022-03-29 | 嘉兴智瞳科技有限公司 | Imaging system with 3D imaging and fluorescence imaging functions |
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