CN113063766A - Micro-droplet fluorescence signal detection device containing biconvex lens - Google Patents
Micro-droplet fluorescence signal detection device containing biconvex lens Download PDFInfo
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- CN113063766A CN113063766A CN202110336727.7A CN202110336727A CN113063766A CN 113063766 A CN113063766 A CN 113063766A CN 202110336727 A CN202110336727 A CN 202110336727A CN 113063766 A CN113063766 A CN 113063766A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention provides a micro-droplet fluorescence signal detection device of a biconvex lens, which comprises a light source module, a light source module and a light source module, wherein the light source module is used for synthesizing different lasers with different wavelengths into mixed excitation light; the light combination module comprises an objective lens, a first dichroic mirror, a first convex lens, a small hole and a second convex lens; the first dichroic mirror reflects the mixed exciting light emitted by the light source module into the objective lens, and the objective lens can focus the mixed exciting light on the micro-droplet to be detected; the first convex lens, the small hole and the second convex lens collimate the plurality of different fluorescent signals into parallel light; and the signal receiving module is connected to the output end of the light combination module and used for receiving the fluorescent signal output by the light combination module. The invention adopts the light path conducting structure of the double-lens single-pinhole to form collimated parallel light, does not influence signal detection because of the increase of the light path, can effectively reduce the loss of fluorescent signals, has only one pinhole without increasing along with the increase of the number of detection channels, and greatly reduces the difficulty of installation and debugging.
Description
Technical Field
The invention belongs to the technical field of microfluidic detection chips, and particularly relates to a micro-droplet fluorescence signal detection device with a biconvex lens.
Background
The biochip has wide application in new medicine development, disease diagnosis, gene expression analysis, etc. The technology of the microfluidic detection chip is mature day by day and becomes a focus of people. There are various biological and chemical processes in the microfluidic detection chip, and the processes are usually completed in a micro-scale flow channel space, in which some devices capable of detecting the reaction process are required.
The detection means can be divided into two types, namely CCD time-sharing shooting and laser single-point frequency division detection, and compared with a laser single-point scanning system, CCD shooting has the advantages of simple structure, short development period and low technical difficulty, but due to the disadvantages of low detection speed, high cost, low sensitivity, large occupied space and the like, the CCD shooting is limited by the diversity of biological samples, the performance of instruments and the like in future application, and is difficult to be applied in large-scale batch. And traditional confocal detection device of laser, the interference of stray light outside the detection light path for eliminating the focal plane, improve detection sensitivity, set up the pinhole at the receiving terminal, its spatial structure mode is single and fixed, no matter be single laser, double laser or many laser arouse signal detection device and all have the fixed increase of pinhole quantity, the laser quantity that is used for arousing the light increases the optical path and increases, detection device follows the shortcoming such as laser to collect that signal end wholeness requires height, the degree of difficulty increases when leading to the debugging because the increase of pinhole quantity, the signal reception error of device has been increased in intangible, carry out multichannel signal detection time measuring, because the space optical path increases and leads to partly signal attenuation and loss, corresponding detected signal existence is lost, the wholeness requires to be highly be not convenient for the equipment and the later maintenance of product.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is to provide a micro-droplet fluorescence signal detection device with a double-lens and a single-aperture, which can form collimated parallel light by using a light path conducting structure with a double-lens and a single-aperture, and can effectively reduce the loss of fluorescence signals without affecting signal detection due to the increase of light path, and the number of apertures is only one without increasing with the increase of the number of detection channels, thereby greatly reducing the difficulty of installation and debugging.
In order to solve the above problems, the present invention provides a micro-droplet fluorescence signal detection apparatus of a lenticular lens, comprising:
the light source module is used for synthesizing different lasers with different wavelengths into mixed exciting light;
the light combination module comprises an objective lens, a first dichroic mirror, a first convex lens, a small hole and a second convex lens; the first dichroic mirror reflects the mixed excitation light emitted by the light source module into the objective lens, and the objective lens can focus the mixed excitation light on micro-droplets to be detected so as to excite and generate a plurality of different fluorescence signals corresponding to different lasers; the first convex lens, the small hole and the second convex lens collimate the plurality of different fluorescent signals into parallel light;
and the signal receiving module is connected to the output end of the light combining module and is used for receiving the fluorescent signal output by the light combining module.
Preferably, the light source module includes a first laser, a second laser, a third laser, a second dichroic mirror, a third dichroic mirror, and a reflecting mirror, and different laser lights respectively emitted by the first laser, the second laser, and the third laser are synthesized into the mixed excitation light through the second dichroic mirror, the third dichroic mirror, and the reflecting mirror.
Preferably, the light source module further includes a light combining cassette and a mounting substrate, the first laser, the second laser, the third laser and the light combining cassette are fixedly connected to the mounting substrate, the second dichroic mirror, the third dichroic mirror and the reflecting mirror are disposed in the light combining cassette, and the light combining cassette has a mixed excitation light exit port and an entrance port for each excitation light.
Preferably, the position of the objective lens in the light combining module can be adjusted under the driving of a focusing motor.
Preferably, the first dichroic mirror is clamped between the first connecting piece, the second connecting piece and the light eliminator; the first convex lens is clamped between the first convex lens connecting piece and the first lens locking nut, and the first convex lens is arranged on one side close to the objective lens; the second convex lens is arranged on a second convex lens connecting piece, and the second convex lens connecting piece is arranged on a third convex lens connecting piece; the small hole is clamped between the fixing plate and a third convex lens connecting piece, wherein the third convex lens connecting piece is positioned on one side far away from the objective lens; the first convex lens connecting piece is arranged on the fixing plate and is positioned at one side close to the objective lens.
Preferably, a first dichroic mirror rubber pad is further clamped between the first connecting piece and the second connecting piece; and/or a convex lens light-tight pad is clamped between the first convex lens connecting piece and the first lens locking nut.
Preferably, the signal receiving module is provided with a first photomultiplier for receiving the first fluorescence signal, a second photomultiplier for receiving the second fluorescence signal, a third photomultiplier for receiving the third fluorescence signal, a fourth photomultiplier for receiving the fourth fluorescence signal, and a fifth photomultiplier for receiving the fifth fluorescence signal.
Preferably, the signal receiving module is further provided with a fourth dichroic mirror, a fifth dichroic mirror, a sixth dichroic mirror and a seventh dichroic mirror, wherein the fourth dichroic mirror separates the fluorescent signal output by the output end of the light combining module into a first path of signal and a second path of signal, the fifth dichroic mirror separates the second path of signal into a third path of signal and a fourth path of signal, the third path of signal is received by the first photomultiplier tube, the fourth path of signal is received by the second photomultiplier tube, the sixth dichroic mirror separates the second path of signal into a fifth path of signal and a sixth path of signal, the fifth path of signal is received by the third photomultiplier tube, the sixth path of signal is separated into a seventh path of signal and an eighth path of signal by the seventh dichroic mirror, the seventh path of signal is the fourth photomultiplier tube, and the eighth path signal is received by the fifth photomultiplier tube.
Preferably, a first color filter is arranged on a light path between the fifth dichroic mirror and the first photomultiplier, a second color filter is arranged on a light path between the fifth dichroic mirror and the second photomultiplier, a third color filter is arranged on a light path between the sixth dichroic mirror and the third photomultiplier, a fourth color filter is arranged on a light path between the seventh dichroic mirror and the fourth photomultiplier, and a fifth color filter is arranged on a light path between the seventh dichroic mirror and the fifth photomultiplier.
Preferably, the signal receiving module includes a first fixing member, a second fixing member, a third fixing member, a fourth fixing member, and a fifth fixing member; the first fixing piece and the second fixing piece clamp the fourth dichroic mirror; the second fixing piece and the third fixing piece clamp the sixth dichroic mirror; the seventh dichroic mirror is clamped by the third fixing piece and the fourth fixing piece; the first fixing piece and the fifth fixing piece clamp the fifth dichroic mirror; a second color filter and a photomultiplier rubber pad are clamped between the first fixing piece and the photomultiplier first connecting piece, and the second color filter is placed in the central circular hole of the first fixing piece; a third color filter and a photomultiplier rubber pad are clamped between the second fixing piece and the photomultiplier second connecting piece, and the third color filter is placed in a central circular hole of the second fixing piece; a fourth color filter and a photomultiplier rubber pad are clamped between the third fixing piece and the photomultiplier third connecting piece, and the fourth color filter is placed in a central circular hole of the third fixing piece; a fifth color filter and a photomultiplier rubber pad are clamped between the fourth fixing piece and the fourth connecting piece of the photomultiplier, and the fifth color filter is placed in a central circular hole of the fourth fixing piece; a first color filter and a photomultiplier rubber pad are clamped between the fifth fixing piece and the fifth connecting piece of the photomultiplier, and the first color filter is placed in a central circular hole of the fifth fixing piece; the first photomultiplier connecting piece is connected with the second photomultiplier; the second connecting piece of the photomultiplier is connected with the third photomultiplier; the fourth photomultiplier is connected to the third connecting piece of the photomultiplier; the fourth connecting piece of the photomultiplier is connected with the fifth photomultiplier; and the fifth connecting piece of the photomultiplier is connected with the first photomultiplier.
According to the micro-droplet fluorescence signal detection device with the double convex lenses, the double-lens single small holes, namely the light path conducting structure formed by the first convex lens, the small holes and the second convex lens, can form collimated parallel light, signal detection cannot be influenced due to increase of the light path, loss of fluorescence signals can be effectively reduced, the number of the small holes is only one, the increase of the number of detection channels is not needed, the difficulty in installation and debugging is greatly reduced, and the detection efficiency is improved; the micro-droplet fluorescence signal detection device of the biconvex lens is divided into the light source module, the light combining module and the signal receiving module from a laser to a signal collecting end, so that the device is more convenient to mount and debug.
Drawings
Fig. 1 is a schematic diagram of a fluorescence signal detection device for micro-droplets of a lenticular lens according to an embodiment of the present invention (arrows in the diagram show light transmission paths);
FIG. 2 is a schematic structural diagram of a fluorescence signal detection device for micro-droplets of a lenticular lens according to an embodiment of the present invention;
fig. 3 is a schematic disassembled structure view of the light source module in fig. 2;
fig. 4 is a schematic view of a disassembled structure of the light combining module in fig. 2;
fig. 5 is a disassembled structural diagram of the signal receiving module in fig. 2.
The reference numerals are represented as:
1. a light source module; 11. a first laser; 12. a second laser; 13. a third laser; 14. a light-combining cassette; 141. a second dichroic mirror; 142. a third dichroic mirror; 143. a mirror; 15. a mounting substrate; 2. a light combining module; 21. an objective lens; 211. a focus motor; 22. a first dichroic mirror; 221. a first connecting member; 222. a first dichroic mirror rubber pad; 223. a second connecting member; 224. a light extinction device; 23. a first convex lens; 231. a first convex lens connecting member; 232. a convex lens light-tight pad; 233. a first lens lock nut; 24. a small hole; 241. a fixing plate; 25. a second convex lens; 251. a second convex lens connecting member; 252. a third convex lens connecting member; 3. a signal receiving module; 33. a fourth dichroic mirror; 331. a first fixing member; 332. a second fixing member; 34. a sixth dichroic mirror; 341. a third photomultiplier tube; 3411. a third color filter; 3413. a photomultiplier tube second connector; 35. a seventh dichroic mirror; 351. a fourth photomultiplier tube; 3511. a fourth color filter; 3513. a third photomultiplier tube connection; 352. a fifth photomultiplier tube; 3521. a fifth color filter; 3523. a fourth connecting piece of the photomultiplier; 353. a fourth fixing member; 354. a second dichroic mirror rubber pad; 355. a third fixing member; 36. a fifth dichroic mirror; 361. a first photomultiplier tube; 3611. a first color filter; 3613. a fifth photomultiplier tube connection; 362. a second photomultiplier tube; 3621. a second color filter; 3623. a photomultiplier tube first connection; 363. a fifth fixing member; 37. photomultiplier rubber pad; 100. micro-droplets are detected.
Detailed Description
Referring to fig. 1 to 5 in combination, according to an embodiment of the present invention, there is provided a micro-droplet fluorescence signal detection apparatus of a lenticular lens, including:
the light source module 1 is used for synthesizing different lasers with different wavelengths into mixed excitation light;
the light combination module 2 comprises an objective lens 21, a first dichroic mirror 22, a first convex lens 23, a small hole 24 and a second convex lens 25; the first dichroic mirror 22 reflects the mixed excitation light emitted by the light source module 1 into the objective lens 21, and the objective lens 21 can focus the mixed excitation light on the micro-droplet 100 to be detected so as to excite and generate a plurality of different fluorescence signals corresponding to different lasers; the first convex lens 23, the small hole 24 and the second convex lens 25 collimate the plurality of different fluorescent signals into parallel light;
and the signal receiving module 3 is connected to the output end of the light combining module 2 and is used for receiving the fluorescent signal output by the light combining module 2.
The technical scheme adopts a double-lens single small hole, namely a light path conducting structure formed by the first convex lens 23, the small hole 24 and the second convex lens 25, and can form collimated parallel light, signal detection cannot be influenced due to increase of light path, loss of fluorescent signals can be effectively reduced, the number of small holes is only one, and the number of small holes does not need to be increased along with increase of the number of detection channels, so that the installation and debugging difficulty is greatly reduced, and the detection efficiency is improved; in addition, the micro-droplet fluorescence signal detection device of the biconvex lens is divided into the light source module 1, the light combining module 2 and the signal receiving module 3 from the laser to the signal collecting end, so that the device is more convenient to mount and debug.
The aperture of the small hole 24 may be from 1 μm to 5 mm, or may be other optical components such as an aperture stop, and similarly, the material of the small hole 24 may be a single material or a polymer composite material, and specifically, a material such as stainless steel, aluminum, copper, or ceramic may be used. The first convex lens 23 and the second convex lens 25 may be in the form of one of other optical structures with focusing functions, such as a biconvex lens, a plano-convex lens, a meniscus lens, a fresnel lens, and the like; the focal length may be any focal length in the range of 1 mm to 10 m, and the material of the first convex lens 23 and the second convex lens 25 may be a single material, or may be a combination material of a cemented lens, such as PMMA, PC, PP, COC, COP, CBC, silica gel, flint glass, crown glass, lanthanum crown glass, quartz, or any combination material thereof, which has high optical transmittance.
Preferably, the light source module 1 includes a first laser 11, a second laser 12, a third laser 13, a second dichroic mirror 141, a third dichroic mirror 142, and a reflecting mirror 143, the laser beams emitted from the first laser 11, the second laser 12, and the third laser 13 are combined into the mixed excitation light by the second dichroic mirror 141, the third dichroic mirror 142, and the reflecting mirror 143, and further, the light source module 1 further includes a light combining cassette 14, and a mounting substrate 15, the first laser 11, the second laser 12, the third laser 13, and the light combining cassette 14 are fixedly connected to the mounting substrate 15, the second dichroic mirror 141, the third dichroic mirror 142, and the reflecting mirror 143 are disposed in the light combining cassette 14, and the light combining cassette 14 has an entrance port for mixing the excitation light and entrance ports for the excitation light, so that the light source module 1 is integrally configured, the structure of the micro-droplet four-fluorescence signal detection device is further compact. The light source of the light source module 1 may be a lighting device having radiation energy, such as a space laser, a fiber laser, an LED, a MICIR-LED, an LCD, a xenon lamp, or a mercury lamp. In a specific embodiment, the first laser 11 is a 473nm laser, the second laser 12 is a 532nm laser, and the third laser 13 is a 637nm laser.
Preferably, the position of the objective lens 21 in the light combining module 2 can be adjusted by driving the focusing motor 211. Specifically, the focusing motor 211 may be a fixed shaft motor, and when the focusing motor 211 operates, the fixed shaft generates an axial displacement, so that the position of the objective lens 21 is adjusted, that is, the displacement generated by the focusing motor 211 is coaxial with the axial direction of the objective lens 21. The objective lens 21 includes, but is not limited to, objective lenses having focal lengths of 4X, 6X, 8X, 16X, 20X, 40X, 100X, and 200X, and may also include optical components or component groups having a magnifying function, such as a self-designed lens or lens group.
As a design manner with a compact structure, the first dichroic mirror 22 is sandwiched between the first connecting piece 221, the second connecting piece 223, and the optical attenuator 224; the first convex lens 23 is clamped between the first convex lens connecting piece 231 and the first lens locking nut 233, and the first convex lens 23 is arranged on one side close to the objective lens 21; the second convex lens 25 is mounted on a second convex lens connecting member 251, and the second convex lens connecting member 251 is mounted on a third convex lens connecting member 252; the small hole 24 is clamped between the fixing plate 241 and a third convex lens connector 252, wherein the third convex lens connector 252 is located on a side far away from the objective lens 21; the first convex lens link 231 is mounted on the fixing plate 241 at a side close to the objective lens 21. Further, a first dichroic mirror rubber pad 222 is further interposed between the first connecting piece 221 and the second connecting piece 223; and/or a convex lens light-tight pad 232 is further clamped between the first convex lens connecting piece 231 and the first lens locking nut 233.
In some embodiments, the signal receiving module 3 is provided with a first photomultiplier 361 for receiving the first fluorescence signal, a second photomultiplier 362 for receiving the second fluorescence signal, a third photomultiplier 341 for receiving the third fluorescence signal, a fourth photomultiplier 351 for receiving the fourth fluorescence signal, and a fifth photomultiplier 352 for receiving the fifth fluorescence signal, so that multiple detection parameters can be obtained by a single detection, the detection result is enriched, the application scenarios and fields are wider, and the experimental cost can be greatly reduced and the detection work efficiency can be improved. Specifically, the signal receiving module 3 is further provided with a fourth dichroic mirror 33, a fifth dichroic mirror 36, a sixth dichroic mirror 34, and a seventh dichroic mirror 35, wherein the fourth dichroic mirror 33 separates the fluorescent signal output from the output end of the light combining module 2 into a first path of signal and a second path of signal, the fifth dichroic mirror 36 separates the second path of signal into a third path of signal and a fourth path of signal, the third path of signal is received by the first photomultiplier 361, the fourth path of signal is received by the second photomultiplier 362, the sixth dichroic mirror 34 separates the second path of signal into a fifth path of signal and a sixth path of signal, the fifth path of signal is received by the third photomultiplier 341, and the sixth path of signal is separated into a seventh path of signal and an eighth path of signal by the seventh dichroic mirror 35, the seventh signal is received by the fourth photomultiplier 351 and the eighth signal is received by the fifth photomultiplier 352. Further, a first color filter 3611 is disposed on a light path between the fifth dichroic mirror 36 and the first photomultiplier 361, a second color filter 3621 is disposed on a light path between the fifth dichroic mirror 36 and the second photomultiplier 362, a third color filter 3411 is disposed on a light path between the sixth dichroic mirror 34 and the third photomultiplier 341, a fourth color filter 3511 is disposed on a light path between the seventh dichroic mirror 35 and the fourth photomultiplier 351, and a fifth color filter 3521 is disposed on a light path between the seventh dichroic mirror 35 and the fifth photomultiplier 352.
In a specific structural design, the signal receiving module 3 includes a first fixing member 331, a second fixing member 332, a third fixing member 355, a fourth fixing member 353, and a fifth fixing member 363; the first fixing member 331 and the second fixing member 332 sandwich the fourth dichroic mirror 33; the second fixing member 332 and the third fixing member 355 clamp the sixth dichroic mirror 34; third holder 355 and fourth holder 353 hold seventh dichroic mirror 35; the first fixing piece 331 and the fifth fixing piece 363 clamp the fifth dichroic mirror 36; a second color filter 3621 and a photomultiplier rubber pad 37 are clamped between the first fixing member 331 and the photomultiplier first connecting member 3623, and the second color filter 3621 is placed in a central circular hole of the first fixing member 331; a third color filter 3411 and a photomultiplier rubber pad 37 are sandwiched between the second fixing member 332 and the photomultiplier second connecting member 3413, and the third color filter 3411 is placed in the central circular hole of the second fixing member 332; a fourth color filter 3511 and a photomultiplier rubber pad 37 are clamped between the third fixing member 355 and the photomultiplier third connecting member 3513, and the fourth color filter 3511 is placed in a central circular hole of the third fixing member 355; a fifth color filter 3521 and a photomultiplier rubber pad 37 are clamped between the fourth fixing piece 353 and the photomultiplier fourth connecting piece 3523, and the fifth color filter 3521 is placed in a central circular hole of the fourth fixing piece 353; a first color filter 3611 and a photomultiplier rubber pad 37 are sandwiched between the fifth fixing element 363 and the photomultiplier fifth connecting element 3613, and the first color filter 3611 is placed in a central circular hole of the fifth fixing element 363; the second photomultiplier 362 is connected to the first photomultiplier tube connector 3623; the third photomultiplier tube 341 is connected to the second photomultiplier tube connecting part 3413; the fourth photomultiplier tube 351 is connected to the third photomultiplier tube connecting part 3513; the fifth photomultiplier tube 352 is connected to the fourth photomultiplier tube connecting part 3523; the fifth photomultiplier 3613 is connected to the first photomultiplier 361, so that the structure is simpler and more compact.
It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims (10)
1. A micro-droplet fluorescence signal detection device with a biconvex lens is characterized by comprising:
the light source module (1) is used for synthesizing different lasers with different wavelengths into mixed excitation light;
the light combination module (2) comprises an objective lens (21), a first dichroic mirror (22), a first convex lens (23), a small hole (24) and a second convex lens (25); the first dichroic mirror (22) reflects the mixed excitation light emitted by the light source module (1) into the objective lens (21), and the objective lens (21) can focus the mixed excitation light on the micro-droplet (100) to be detected so as to excite and generate a plurality of different fluorescence signals corresponding to different lasers; the first convex lens (23), the small hole (24) and the second convex lens (25) collimate the plurality of different fluorescent signals into parallel light;
and the signal receiving module (3) is connected to the output end of the light combining module (2) and is used for receiving the fluorescent signal output by the light combining module (2).
2. The micro-droplet fluorescence signal detection device according to claim 1, wherein the light source module (1) comprises a first laser (11), a second laser (12), a third laser (13), a second dichroic mirror (141), a third dichroic mirror (142), and a reflecting mirror (143), and different lasers emitted by the first laser (11), the second laser (12), and the third laser (13) are combined into the mixed excitation light through the second dichroic mirror (141), the third dichroic mirror (142), and the reflecting mirror (143).
3. The microdroplet fluorescence signal detection device according to claim 2, wherein the light source module (1) further comprises a light combining cassette (14) and a mounting substrate (15), the first laser (11), the second laser (12), the third laser (13) and the light combining cassette (14) are fixedly connected to the mounting substrate (15), the second dichroic mirror (141), the third dichroic mirror (142) and the reflecting mirror (143) are disposed in the light combining cassette (14), and the light combining cassette (14) has a mixed excitation light outlet and an incident port for each excitation light.
4. The microdroplet fluorescence signal detection device according to claim 1, characterized in that the position of the objective lens (21) in the light combination module (2) can be adjusted by driving a focusing motor (211).
5. The micro-droplet fluorescence signal detection device according to claim 1, wherein the first dichroic mirror (22) is sandwiched between a first connector (221), a second connector (223), and a light eliminator (224); the first convex lens (23) is clamped between the first convex lens connecting piece (231) and the first lens locking nut (233), and the first convex lens (23) is arranged on one side close to the objective lens (21); the second convex lens (25) is mounted on a second convex lens connector (251), and the second convex lens connector (251) is mounted on a third convex lens connector (252); the small hole (24) is clamped between the fixing plate (241) and a third convex lens connecting piece (252), wherein the third convex lens connecting piece (252) is positioned on one side far away from the objective lens (21); the first convex lens connector (231) is mounted on the fixing plate (241) and is positioned at one side close to the objective lens (21).
6. The micro-droplet fluorescence signal detection device according to claim 5, wherein a first dichroic mirror rubber pad (222) is further sandwiched between the first connecting piece (221) and the second connecting piece (223); and/or a convex lens light-tight pad (232) is clamped between the first convex lens connecting piece (231) and the first lens locking nut (233).
7. The detection apparatus for fluorescence signals from micro-droplets according to claim 1, wherein the signal receiving module (3) is provided with a first photomultiplier (361) for receiving a first fluorescence signal, a second photomultiplier (362) for receiving a second fluorescence signal, a third photomultiplier (341) for receiving a third fluorescence signal, a fourth photomultiplier (351) for receiving a fourth fluorescence signal, and a fifth photomultiplier (352) for receiving a fifth fluorescence signal.
8. The micro-droplet fluorescence signal detection apparatus according to claim 7, wherein a fourth dichroic mirror (33), a fifth dichroic mirror (36), a sixth dichroic mirror (34), and a seventh dichroic mirror (35) are further disposed in the signal receiving module (3), wherein the fourth dichroic mirror (33) separates the fluorescence signal output from the output end of the light combining module (2) into a first path of signal and a second path of signal, the fifth dichroic mirror (36) separates the second path of signal into a third path of signal and a fourth path of signal, wherein the third path of signal is received by the first photomultiplier tube (361), the fourth path of signal is received by the second photomultiplier tube (362), and the sixth dichroic mirror (34) separates the second path of signal into a fifth path of signal and a sixth path of signal, wherein the fifth path of signal is received by the third photomultiplier tube (341), the sixth signal is separated by the seventh dichroic mirror (35) into a seventh signal and an eighth signal, wherein the seventh signal is received by the fourth photomultiplier (351) and the eighth signal is received by the fifth photomultiplier (352).
9. The micro-droplet fluorescence signal detection device according to claim 8, wherein a first color filter (3611) is disposed on a light path between the fifth dichroic mirror (36) and the first photomultiplier (361), a second color filter (3621) is disposed on a light path between the fifth dichroic mirror (36) and the second photomultiplier (362), a third color filter (3411) is disposed on a light path between the sixth dichroic mirror (34) and the third photomultiplier (341), a fourth color filter (3511) is disposed on a light path between the seventh dichroic mirror (35) and the fourth photomultiplier (351), and a fifth color filter (3521) is disposed on a light path between the seventh dichroic mirror (35) and the fifth photomultiplier (352).
10. The micro-droplet fluorescence signal detection device according to claim 9, wherein the signal receiving module (3) comprises a first fixing member (331), a second fixing member (332), a third fixing member (355), a fourth fixing member (353), and a fifth fixing member (363); the fourth dichroic mirror (33) is clamped by the first fixing piece (331) and the second fixing piece (332); the sixth dichroic mirror (34) is clamped by the second fixing piece (332) and the third fixing piece (355); the seventh dichroic mirror (35) is clamped by the third fixing member (355) and the fourth fixing member (353); the first fixing piece (331) and the fifth fixing piece (363) clamp the fifth dichroic mirror (36); a second color filter (3621) and a photomultiplier rubber pad (37) are clamped between the first fixing piece (331) and the photomultiplier first connecting piece (3623), and the second color filter (3621) is placed in a central circular hole of the first fixing piece (331); a third color filter (3411) and a photomultiplier rubber pad (37) are clamped between the second fixing part (332) and the photomultiplier second connecting piece (3413), and the third color filter (3411) is placed in a central circular hole of the second fixing part (332); a fourth color filter (3511) and a photomultiplier rubber pad (37) are clamped between the third fixing piece (355) and the photomultiplier third connecting piece (3513), and the fourth color filter (3511) is placed in a central circular hole of the third fixing piece (355); a fifth color filter (3521) and a photomultiplier rubber pad (37) are clamped between the fourth fixing piece (353) and the fourth connecting piece (3523) of the photomultiplier, and the fifth color filter (3521) is placed in a central circular hole of the fourth fixing piece (353); a first color filter (3611) and a photomultiplier rubber pad (37) are clamped between the fifth fixing piece (363) and the photomultiplier fifth connecting piece (3613), and the first color filter (3611) is placed in a central circular hole of the fifth fixing piece (363); the photomultiplier tube first connector (3623) is connected with the second photomultiplier tube (362); the third photomultiplier (341) is connected to the second photomultiplier connecting member (3413); the fourth photomultiplier (351) is connected to the third photomultiplier connecting piece (3513); the fourth connecting piece (3523) of the photomultiplier is connected with the fifth photomultiplier (352); the fifth connecting piece (3613) of the photomultiplier is connected with the first photomultiplier (361).
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