CN214457921U

CN214457921U - Neuron cell culture plate

Info

Publication number: CN214457921U
Application number: CN202120448941.7U
Authority: CN
Inventors: 刘俊; 于丽娜; 刘瑞婷
Original assignee: Shandong Shengqiangde Biotechnology Co ltd
Current assignee: Shandong Shengqiangde Biotechnology Co ltd
Priority date: 2021-03-02
Filing date: 2021-03-02
Publication date: 2021-10-22
Anticipated expiration: 2031-03-02

Abstract

The utility model discloses a neuron cell culture plate relates to cell cultivation technical field. This neuron cell culture plate includes outer box, lid, baffle and culture plate, and the fixed surface joint of the upper surface of outer box has the lid, the interior bottom surface fixedly connected with baffle of outer box. This neuron cell culture board passes through the fixed joint culture plate of fixture block at the culture plate box body inner wall among the prior art, is using the utility model discloses during the device, outer box inner wall is separated through the baffle and is independent culture space when need add the nutrient solution to the culture medium midway, and the burette only need lean on and add at the draft tube lateral wall, and the nutrient solution is in the same direction as draft tube and is slowly getting into the culture medium, can not produce too violent shock to the cell crowd that has proliferated in former culture medium, just the utility model discloses the device has two-layer proliferation space and uses as cell proliferation, can select the user mode that accords with the use most according to the actual use condition, and culture dish matrix quantity and position can be controlled at will simultaneously.

Description

Neuron cell culture plate

Technical Field

The utility model relates to a cell cultivation technical field specifically is a neuron cell culture plate.

Background

Cell culture refers to a technique of placing collected in vivo tissues under sterile, certain nutritional conditions, proper temperature and pH value to grow and propagate the tissues and maintain the structures and functions of the tissues, and the cell culture can directly observe the morphological structure, the life activities and the changes of living cells and has important effects on the research and the application of cytology, genetics, virology and immunology.

The cell culture plate is the most common cell culture apparatus in a laboratory, the number, quality and uniformity of monolayer cells are directly related to subsequent experimental results, the proliferation of isolated cells is carried out in a culture solution, a culture medium is frequently added to the culture plate according to the proliferation requirement, in order to avoid damaging the growth of cell clusters in the process of adding the culture medium, a dropper is required to be attached to the inner wall of the culture plate to slowly add the culture medium, the pipette is required to be frequently replaced in the process, the possibility of pollution of the culture medium is still increased, and the technical personnel in the field of the meaning provide a neuron cell culture plate to solve the problems.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

To the not enough of prior art, the utility model discloses a neuron cell culture plate to solve the problem that proposes in the above-mentioned background art.

(II) technical scheme

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides a neuron cell culture plate, includes outer box, lid, baffle and culture plate, the last fixed surface joint of outer box has the lid, the interior bottom surface fixedly connected with baffle of outer box, the inner wall fixed joint of outer box has the culture plate, the culture plate includes L shape fixture block, layer board, draft tube, culture dish base member, the inner wall symmetry fixedly connected with L shape fixture block of outer box, the side fixed joint of L shape fixture block has the layer board, the lower fixed surface of layer board is connected with the draft tube, the inboard fixed joint of draft tube has the culture dish base member.

Preferably, the side of the top end of the outer box body is provided with a connecting groove, the bottom end of the box cover is fixedly connected with a clamping ring matched with the connecting groove, and a sealing ring is arranged on the inner side of the clamping ring.

Preferably, the two partition plates are perpendicular to each other and fixedly connected to the inner bottom surface of the outer box body, and two opposite side surfaces of the partition plates are fixedly connected to the inner wall of the outer box body.

Preferably, an L-shaped groove is formed in the side face of the L-shaped clamping block, a connecting block is slidably connected to the inner side of the L-shaped groove, a limiting block matched with the supporting plate is fixedly connected to one end of the connecting block, the other end of the connecting block penetrates through the upper surface of the L-shaped clamping block and extends to the position above the L-shaped clamping block, and a clamping spring is fixedly connected to one side, far away from the supporting plate, of the connecting block.

Preferably, the upper surface of the supporting plate is provided with a clamping groove matched with the culture dish base body at the position of the guide cylinder, and the guide cylinder extends to a position close to the inner bottom surface of the outer box body.

Preferably, the side fixedly connected with on culture dish base member top and the spacing ring of draw-in groove looks adaptation, the lower surface symmetry fixedly connected with lug of outer box body, the upper surface symmetry fixedly connected with of lid and the gag lever post of lug looks adaptation, the side fixedly connected with reset spring of gag lever post.

The utility model discloses a neuron cell culture plate, its beneficial effect who possesses as follows:

1. this neuron cell culture plate, the culture plate box body inner wall among the prior art passes through the fixed joint culture plate of fixture block, is using the utility model discloses during the device, outer box inner wall separates for independent culture space through the baffle when need add the nutrient solution to the culture medium midway, and the burette only needs to lean on and adds at the draft tube lateral wall, and the nutrient solution is in the same direction as draft tube and slowly gets into the culture medium, can not produce too violent shock to the cell crowd that has proliferated in former culture medium.

2. This neuron cell culture board, the fixed joint culture plate of box inner wall outside, and culture dish base member can be according to in service behavior selection whether install and installation quantity on the culture plate, make on the one hand the utility model discloses the device can regard as regional minizone cell difference proliferation experiment again to use when scope nature cell proliferation container uses, realizes the nimble application of same device realization multiple use to culture dish base member on the culture plate can the interval set up, and as the contrast experiment of cell proliferation experiment under the different environment, the regional constant temperature material that fills of lower floor's baffle provides experimental environment, increases the utility model discloses the in-service use scope of device.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of the structure A of FIG. 1 according to the present invention;

FIG. 3 is a sectional view of the overall structure of the present invention;

FIG. 4 is a top view of the outer box structure of the present invention;

fig. 5 is an enlarged view of a structure B in fig. 4 according to the present invention.

In the figure: 1. an outer case; 2. a box cover; 3. a partition plate; 4. culturing the plate; 401. an L-shaped fixture block; 402. a support plate; 403. a draft tube; 404. a culture dish substrate; 5. connecting grooves; 6. a snap ring; 7. a seal ring; 8. an L-shaped slot; 9. connecting blocks; 10. a limiting block; 11. a tightening spring; 12. a card slot; 13. a limiting ring; 14. a bump; 15. a limiting rod; 16. a return spring.

Detailed Description

The embodiment of the utility model discloses neuron cell culture plate, as shown in fig. 1-5, including outer box 1, lid 2, baffle 3 and culture plate 4, outer box 1's last fixed surface joint has lid 2, outer box 1's interior bottom fixedly connected with baffle 3, two 3 mutually perpendicular fixed connections of baffle are at outer box 1's interior bottom surface, two sides that baffle 3 is relative and outer box 1 inner wall fixed connection, outer box 1's inner wall fixed joint has culture plate 4, spread groove 5 has been seted up to the side on outer box 1 top, the bottom fixedly connected with of lid 2 and the snap ring 6 of spread groove 5 looks adaptation, the inboard of snap ring 6 is provided with sealing washer 7.

The culture plate 4 comprises an L-shaped fixture block 401, a support plate 402, a guide cylinder 403 and a culture dish substrate 404, the L-shaped fixture block 401 is symmetrically and fixedly connected to the inner wall of the outer box body 1, the support plate 402 is fixedly clamped on the side face of the L-shaped fixture block 401, the guide cylinder 403 is fixedly connected to the lower surface of the support plate 402, and the culture dish substrate 404 is fixedly clamped on the inner side of the guide cylinder 403.

L-shaped groove 8 has been seted up to L-shaped fixture block 401's side, and the inboard sliding connection in L-shaped groove 8 has connecting block 9, and the stopper 10 of connecting block 9's one end fixedly connected with and layer board 402 looks adaptation, the connecting block 9 other end run through the upper surface of L-shaped fixture block 401 and extend to L-shaped fixture block 401 top, and one side fixedly connected with tight position spring 11 that layer board 402 was kept away from to connecting block 9.

The upper surface of the supporting plate 402 and the position of the guide cylinder 403 are provided with a clamping groove 12 matched with the culture dish substrate 404, and the guide cylinder 403 extends to be close to the inner bottom surface of the outer box body 1.

The side fixedly connected with on culture dish base member 404 top and draw-in groove 12 looks adaptation stop collar 13, the lower surface symmetry fixedly connected with lug 14 of outer box 1, the upper surface symmetry fixedly connected with of lid 2 and the gag lever post 15 of lug 14 looks adaptation, the side fixedly connected with reset spring 16 of gag lever post 15.

The working principle is as follows: the utility model discloses the device is in the in-service use, if need do the external multiplication on a large scale, will the utility model discloses culture dish base member 404 on the culture plate 4 in the device is got rid of, directly breeds the cultivation in 1 region of the outer box body by 3 divided baffles, if add nutrient solution to the culture dish in observing needs this moment, only need lean on the lateral wall at draft tube 403 with the burette and add, nutrient solution moves to being close culture medium department along the lateral wall of draft tube 403 and drips, and it is less to the influence of proliferated cell crowd in the culture medium.

When cells are proliferated in a contrast experiment or in a small range in use, proliferation culture dishes separated by the partition plate 3 are not needed, a proper amount of culture dish matrixes 404 are fixedly clamped on the supporting plate 402 according to use requirements, the guide cylinder 403 is matched with the clamping groove 12 to fix the culture dish matrixes 404 on the supporting plate 402, the lower culture dish can be filled with constant-temperature matrixes or other auxiliary articles, the lower culture dish can be used as a double layer without influencing the use of the two culture dishes, and the nutrient solution can be still added to the lower culture dish through the upper guide cylinder 403.

When layer board 402 blocks with L-shaped fixture block 401, layer board 402 exerts pressure to stopper 10, pressure pushes stopper 10 to connecting block 9 direction, and the tight position spring 11 of compression connecting block 9 side fixed connection takes place deformation, after layer board 402 moved to stopper 10 below, tight position spring 11 withdrawed from stopper 10 through connecting block 9, it is spacing to layer board 402, and only need stir the tight position spring 11 of the outstanding L-shaped fixture block 401 part compression of connecting block 9 with the finger when needing to take out layer board 402, can take out layer board 402.

Often need pile up a plurality of devices in the thermostat in the proliferation is used, if the relation that only simply piles up between two devices this moment, cause very easily when rocking to pile up 4 slippage of culture plate and collapse, cause acutely rocking of inside culture medium, be unfavorable for the external proliferation of cell, the utility model discloses lug 14 and the gag lever post 15 that the surface symmetry set up on 2 of lid of outer box body 1 lower surface of device increase the device wholeness and shake the ability with two adjacent device fixed joint, are favorable to piling up the operation of multilayer culture plate 4.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a neuron cell culture plate, includes outer box body (1), lid (2), baffle (3) and culture plate (4), its characterized in that: the upper surface fixed joint of outer box (1) has lid (2), interior bottom fixed connection of outer box (1) has baffle (3), the inner wall fixed joint of outer box (1) has culture plate (4), culture plate (4) are including L shape fixture block (401), layer board (402), draft tube (403), culture dish base member (404), the inner wall symmetry fixedly connected with L shape fixture block (401) of outer box (1), the side fixed joint of L shape fixture block (401) has layer board (402), the lower fixed surface of layer board (402) is connected with draft tube (403), the inboard fixed joint of draft tube (403) has culture dish base member (404).

2. The neuronal cell culture plate according to claim 1, wherein: the side on outer box (1) top has seted up spread groove (5), bottom fixedly connected with of lid (2) and spread groove (5) looks adaptation snap ring (6), the inboard of snap ring (6) is provided with sealing washer (7).

3. The neuronal cell culture plate according to claim 1, wherein: the two clapboards (3) are mutually perpendicular and fixedly connected to the inner bottom surface of the outer box body (1), and two opposite side surfaces of the clapboards (3) are fixedly connected with the inner wall of the outer box body (1).

4. The neuronal cell culture plate according to claim 1, wherein: l shape fixture block (401) side seted up L shape groove (8), the inboard sliding connection in L shape groove (8) has connecting block (9), the stopper (10) of the one end fixedly connected with and layer board (402) looks adaptation of connecting block (9), the upper surface that the L shape fixture block (401) was run through to connecting block (9) other end extends to L shape fixture block (401) top, one side fixedly connected with tight position spring (11) of layer board (402) are kept away from in connecting block (9).

5. The neuronal cell culture plate according to claim 1, wherein: the upper surface of the supporting plate (402) is provided with a clamping groove (12) matched with the culture dish base body (404) at the position of the guide cylinder (403), and the guide cylinder (403) extends to a position close to the inner bottom surface of the outer box body (1).

6. The neuronal cell culture plate according to claim 1, wherein: the utility model discloses a culture dish, including culture dish base member (404), draw-in groove (12) looks adaptation, the spacing ring (13) of side fixedly connected with and draw-in groove (12) looks adaptation on culture dish base member (404), the lower surface symmetry fixedly connected with lug (14) of outer box body (1), the gag lever post (15) of the upper surface symmetry fixedly connected with and lug (14) looks adaptation of lid (2), the side fixedly connected with reset spring (16) of gag lever post (15).