CN214218761U - Living cell culture device for super-resolution imaging and lossless transfer module - Google Patents
Living cell culture device for super-resolution imaging and lossless transfer module Download PDFInfo
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- CN214218761U CN214218761U CN202120005003.XU CN202120005003U CN214218761U CN 214218761 U CN214218761 U CN 214218761U CN 202120005003 U CN202120005003 U CN 202120005003U CN 214218761 U CN214218761 U CN 214218761U
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Abstract
The utility model discloses a living cell culture device and harmless transfer module for super-resolution formation of image, include: a top cover module; and a base module comprising a hollow base body having a top cover access end and a bottom end; the top cover access end is detachably connected with the top cover module; the base body is close to the inside wall of bottom is equipped with the slide embedded groove, the slide embedded groove axial link up the terminal surface of bottom forms the through-hole, the edge constitution of slide embedded groove and through-hole constitutes the annular holding surface that the formation of image slide provided ascending holding power. The utility model discloses the slide that can compatible all kinds of specifications carries out cell culture to can carry out the imaging experiment under super-resolution yardstick.
Description
Technical Field
The utility model relates to a living cell culture device especially relates to a living cell culture device and harmless transfer module for super-resolution imaging.
Background
The living cell culture device is an important tool for effectively realizing long-term observation in the field of microscopic imaging. At present, the existing cell culture device is made of plastic mostly and can only be used once, and the plastic material has certain influence on the activity of cells and can not completely keep the good growth state of the cells. With the increasing scientific research demand of living cell imaging, the imaging efficiency of the existing cell culture device is greatly reduced due to the fact that parameters such as batches, quality and the like are different.
Therefore, a culture device which can keep imaging conditions fixed and is suitable for being placed in a living cell culture environment for a long time is needed to meet the requirements of products such as a super-resolution microscope and a mature living cell workstation.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a living cell culture device for super-resolution formation of image, the slide that can compatible all kinds of specifications carries out cell culture to can carry out the formation of image experiment under super-resolution yardstick.
In order to achieve the above object, the present invention provides a living cell culture device for super-resolution imaging, comprising:
a top cover module; and
a base module comprising a hollow base body having a top cover access end and a bottom end;
the top cover access end is detachably connected with the top cover module; the base body is close to the inside wall of bottom is equipped with the slide embedded groove, the slide embedded groove axial link up the terminal surface of bottom forms the through-hole, the edge constitution of slide embedded groove and through-hole constitutes the annular holding surface that the formation of image slide provided ascending holding power.
Further, the size of the through hole is slightly smaller than the size of the imaging slide.
Further, the axial distance between the surface of the imaging slide close to the bottom end and the end face of the bottom end is in the range of 0.1 mm-0.2 mm.
Further, the top cover module comprises a top cover body, the outer side wall of the top cover body is arranged to be a step surface, the step of the top cover module is abutted to the top cover access end when the top cover module is connected to the base module, the outer side wall of the top cover body is axially divided into two sections with different outer diameter sizes, one end, close to the outer side wall with the relatively smaller outer diameter size, of the top cover body is a base joint end, and one end of the top cover body is a handheld end.
Furthermore, a placing groove for placing the sealing ring is formed in the end face of the joint end of the base, and the depth and the width of the placing groove in the axial direction are slightly smaller than the diameter of the sealing ring.
Further, the top cap body is provided with the external screw thread on being close to the relative less lateral wall of external diameter size, the base body close to the inside wall of top cap incoming end be provided with can with thread fit's internal thread.
Further, the inner side wall of the top cover body is arranged to be a step surface, the inner side wall of the top cover body is axially divided into two sections with different outer diameter sizes, and the inner side wall with the relatively larger inner diameter size is arranged close to the handheld end.
Further, the top cap body is close to the inside wall of handheld end sets up to the inclined plane, the normal direction on inclined plane is towards set up outside the top cap body.
Furthermore, a plurality of slotted holes are formed in the end face of the handheld end at intervals, and the slotted holes penetrate through the inclined plane in the radial direction.
The utility model also provides a supplementary as above cell culture device's that lives that is used for super-resolution formation of image harmless transfer module, a serial communication port, harmless transfer module has at least one and places the recess that is used for the cell culture device that lives of super-resolution formation of image, the degree of depth of recess is less than the axial height of the base module that is used for the cell culture device that lives of super-resolution formation of image, and the internal diameter slightly is greater than the external diameter of base module, but the bottom surface of recess is offered the size slightly and is greater than the imaging area of formation of image slide along the direction of depth and is nevertheless less than the through-hole of base module external diameter.
The utility model discloses owing to take above technical scheme, it has following advantage:
1. can effectively maintain the cell activity and the imaging quality under the condition of using a super-resolution microscope.
2. The device is compatible to the use of a living cell workstation device in the imaging process of a super-resolution microscope, and can be repeatedly used, adapted for sterilization, subjected to high temperature and other series of different treatments. The optimization of culture modes such as increasing the contact area of gas, improving the actual cell coating area, storing two-stage liquid and the like ensures the optimization of the growth and proliferation state of living cells under the imaging working condition.
3. The integrity and the imaging tightness of the imaging slide can be guaranteed to the maximum extent, and the influence of the liquid leakage phenomenon on the microscope objective is avoided.
Drawings
Fig. 1 is an exploded view of a living cell culture apparatus for super-resolution imaging according to an embodiment of the present invention.
Fig. 2 is an assembled schematic view of the top cover module and the base module of fig. 1.
Fig. 3 is a schematic cross-sectional view of fig. 2.
Fig. 4 is a schematic view of the top cover module of fig. 1.
Fig. 5 is a schematic cross-sectional view of fig. 4.
Fig. 6 is a schematic view of the base module of fig. 1.
FIG. 7 is a non-destructive transfer module of the super-resolution living cell culture apparatus.
FIG. 8 is a schematic diagram showing the positional relationship between the super-resolution living cell culture apparatus and the nondestructive transfer module.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and examples.
As shown in fig. 1 to 3, a living cell culture apparatus a for super-resolution imaging provided by the embodiment of the present invention includes a top cover module 1 and a base module 2. The top cover module 1 is mainly used for fixing the imaging slide 3 and sealing solution, the top cover module 1 can be detached before imaging, and the imaging slide 3 is installed after being placed in the base module 2.
As shown in fig. 4 and 5, the top cover module 1 has a cylindrical top cover body 11 with two through ends. The cylinder may for example be cylindrical.
The outer side wall of the top cover body 11 is provided as a step face, the step of which abuts against the base module 2 when the top cover module 1 is connected to the base module 2. The outer side wall of the top cover body 11 is axially divided into two sections with different outer diameter dimensions by the step surface of the outer side wall of the top cover body 11. Wherein the outer side wall with a relatively small outer diameter dimension is provided as a cylindrical surface on which an outer thread 12 is provided for threaded connection with the base module 2. Therefore, an end of the cap body 11 adjacent to the external thread 12 is referred to as a base engagement end 1 a. Be provided with annular knurl 13 on the great lateral wall of external diameter size relatively, form unevenness's surface to increase frictional force, conveniently tear open and get. The other end of the cap body 11 adjacent to the knurl 13 is referred to as a hand-held end 1 b. "outside diameter" is understood to mean the circumference of the outside wall rotated one revolution around the central axis of the cap body 11. "axial" refers to the direction of the central axis of the header body 11.
The inner side wall of the header body 11 is provided as a stepped surface so as to divide the inner side wall of the header body 11 into two sections having different outer diameter dimensions along the axial direction of the header body 11. Wherein an inner side wall having a relatively large inner diameter dimension is provided adjacent to the hand-held end 1 b. An inner side wall having a relatively small inner diameter dimension is disposed adjacent to the base engaging end 1 a. "inside diameter" is understood to mean the circumference of the inside wall that rotates one revolution about the central axis of the header body 11. Through the step face of 11 inside walls of top cap body, satisfied the demand that a small amount of liquid culture observed, saved the raw materials. And enough space is reserved for other sample operations, so that the contact area of the culture solution and the gas can be ensured to be large enough under the assembly condition of the living cell workstation, and the survival rate of cell culture is further improved.
The end surface of the base joint end 1a is provided with a placing groove 15 for placing the sealing ring 14, and the depth and the width of the placing groove 15 along the axial direction are slightly smaller than the diameter of the sealing ring 14. Such as: the depth of the placement groove 15 is 0.1mm to 0.5mm smaller than the diameter of the seal ring 14, and the diameter of the placement groove 15 is 0.1mm to 0.5mm smaller than the diameter of the seal ring 14. In this way, on the one hand, the gasket 14 is facilitated to be firmly inserted into the placement groove 15, tightly fitting with the top cover body 11, and on the other hand, the installed gasket 14 protrudes beyond the base joint end 1a, thereby ensuring that the gasket 14 first contacts the imaging slide 3, but not the base joint end 1a, during the approach of the top cover module 1 toward the base module 2. The sealing ring 14 is made of rubber, and elasticity is reserved, and meanwhile the requirement for hardness can be met. After the top cover module 1 is screwed on the base module 2, the sealing ring 14 is tightly attached to the surface of the imaging slide 3, so that the phenomena of liquid leakage and breakage of the imaging slide 3 can be avoided.
The inner side wall adjacent to the handheld end 1b is provided with a slope 16, and the normal direction of the slope 16 is arranged towards the outside of the top cover body 11. The inclined surface 16 facilitates the liquid adding operation under the condition of not conflicting with the position relation of devices around the microscope. "outwardly of the cap body 11" is with respect to the overall structure, and the direction toward the structure is referred to as inwardly and vice versa outwardly.
The end surface of the holding end 1b is provided with a plurality of slotted holes 17 at intervals, and the slotted holes 17 penetrate through the inclined surface 16 along the radial direction. The number of slots 17 may be four in the figure, but is not limited thereto. After long-term use, the four slotted holes 17 can be fastened by an installation tool to increase the torque for auxiliary disassembly. "radial" refers to a direction perpendicular to the central axis of the header body 11.
As shown in fig. 3 and 6, the base module 2 has a cylindrical base body 21 with two through ends, one end of which is a top cover access end 2a, and the other end of which is a bottom end 2 b.
A slide-fitting groove 22 is provided on the inner side wall near the bottom end 2b, and the slide-fitting groove 22 axially penetrates the end face of the bottom end 2b to form a through hole 23. The slide fitting grooves 22 and the edges of the through holes 23 constitute an annular support surface which provides an upward supporting force for the imaging slide 3. The size of the through hole 23 is slightly smaller than the size of the imaging slide 3, so that the area of the imaging slide 3 can be utilized to the maximum. The axial distance between the surface of the imaging slide 3 close to the bottom end 2b and the end face of the bottom end 2b can be controlled within the range of 0.1 mm-0.2 mm through fine machining, so that the precise focusing operation can be realized within the movable range of the imaging objective lens.
The inside wall setting that base body 21 is close to top cap incoming end 2a is the cylindrical surface, has internal thread 24 above that, from this with external screw thread 12 threaded connection, realizes top cap module 1 and base module 2's connection fixed.
Before imaging, the imaging slide 3 can be placed in the slide embedding groove 2, and then the top cover body 11 is screwed with the slide embedding groove through threaded fit. The outer side wall of the base module 2 can also be subjected to knurling operation in the machining process, so that the friction force is increased, and the base module is convenient to disassemble and take.
In one embodiment, the living cell culture device a for super-resolution imaging is made of stainless steel, the surface treatment and finish machining process is adapted to the requirements of the numerical aperture and the refractive index liquid of the microscope, and the elastic line contact form with the imaging slide 3 can also maximally ensure the integrity of the slide and the imaging tightness so as to avoid the influence of the liquid leakage phenomenon on the microscope objective.
In the cell culture process, the imaging slide 3 is always in the culture solution filling environment, when the imaging slide 3 is placed into the living cell culture device A for super-resolution imaging, a small amount of culture solution residues are still left on the bottom surface of the imaging slide, and the operator waits for a long volatilization time to affect the transfer efficiency. Meanwhile, in the process of waiting for the starting of the imaging system, the living cell culture device A for super-resolution imaging also needs to ensure that the bottom surface of the imaging slide 3 is not contacted with impurities and polluted when placed.
As shown in fig. 7 and 8, the nondestructive transfer module B is an auxiliary module of the super-resolution living cell culture apparatus a, and is used for nondestructive transfer and temporary storage thereof, so as to ensure that the bottom end of the imaging slide 3 is not affected by contamination during the transfer and storage process, and ensure the super-resolution imaging quality, and the positional relationship between the nondestructive transfer module B and the super-resolution living cell culture apparatus a is shown in fig. 8.
The whole nondestructive transfer module B is also made of stainless steel, so that the requirements of disinfection and sterilization and reutilization can be met.
The nondestructive transfer module B is provided with at least one groove 4 for placing the super-resolution living cell culture device A, the depth of the groove 4 is less than the axial height of the base module 2, such as: the groove depth of the groove 4 is 2mm to 3mm smaller than the axial height of the base module 2, so that the whole culture device can be conveniently placed into the groove 4. The inner diameter of the groove 4 is slightly larger than the outer diameter of the base module 2, for example, slightly larger than 2 mm-5 mm, so that the whole culture device can be conveniently placed in the groove 4, and the whole culture device can be prevented from shaking left and right after being placed. The bottom surface of the groove 4 is provided with a through hole with the size slightly larger than the imageable area of the imaging slide 3 but smaller than the outer diameter of the base module 2 along the depth direction, wherein the size is slightly larger than 2-5 mm.
Compared with the mode that the cell culture device is directly placed on a desktop or a counter surface for temporary storage, the super-resolution living cell culture device A is placed in the groove 4 of the lossless transfer module B, so that a buffer space is reserved below the imaging slide 3, and the imaging transparency is guaranteed.
The nondestructive transfer module B integrally meets the characteristics of miniaturization and easy carrying, can enable an operator to temporarily store a plurality of super-resolution imaging living cell culture devices at the same time, and is favorable for carrying out large-flux cell imaging experiments.
Finally, it should be pointed out that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it. Those of ordinary skill in the art will understand that: modifications can be made to the technical solutions described in the foregoing embodiments, or some technical features may be equivalently replaced; such modifications or substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (10)
1. A living cell culture device for super-resolution imaging, comprising:
a roof module (1); and
a base module (2) comprising a hollow base body (21) having a top cover access end (2a) and a bottom end (2 b);
wherein the top cover access end (2a) is detachably connected with the top cover module (1); base body (21) are close to the inside wall of bottom (2b) is equipped with slide embedded groove (22), slide embedded groove (22) axial link up the terminal surface of bottom (2b) forms through-hole (23), the edge constitution of slide embedded groove (22) and through-hole (23) constitutes formation of image formation slide (3) provides the annular holding surface of ascending holding power.
2. The device for the culture of living cells for super-resolution imaging according to claim 1, wherein the size of the through hole (23) is slightly smaller than the size of the imaging slide (3).
3. The device for culturing living cells for super-resolution imaging according to claim 2, wherein the axial distance between the surface of the imaging slide (3) adjacent to the bottom end (2b) and the end face of the bottom end (2b) is in the range of 0.1mm to 0.2 mm.
4. The living cell culture apparatus for super resolution imaging according to any one of claims 1 to 3, wherein the roof module (1) comprises a roof body (11), wherein the outer side wall of the roof body (11) is provided as a stepped surface, the step of which abuts against the roof access end (2a) when the roof module (1) is connected to the base module (2), and divides the outer side wall of the roof body (11) into two segments having different outer diameter dimensions along the axial direction, wherein the end of the roof body (11) adjacent to the outer side wall having the relatively smaller outer diameter dimension is a base engagement end (1a), and the end of the roof body (11) is a hand-held end (1 b).
5. The living cell culture device for super-resolution imaging according to claim 4, wherein the end face of the base joint end (1a) is provided with a placement groove (15) for placing the sealing ring (14), and the depth and width of the placement groove (15) along the axial direction are slightly smaller than the diameter of the sealing ring (14).
6. The device for culturing living cells for super-resolution imaging according to claim 5, wherein the cap body (11) is provided with an external thread (12) adjacent to the outer side wall with relatively small outer diameter dimension, and the base body (21) is provided with an internal thread (24) adjacent to the inner side wall of the cap access end (2a) and capable of being matched with the external thread (12).
7. The living cell culturing device for super resolution imaging according to claim 6, wherein the inner side wall of the header body (11) is provided as a stepped surface dividing the inner side wall of the header body (11) into two segments having different outer diameter dimensions in the axial direction, wherein the inner side wall having a relatively larger inner diameter dimension is provided adjacent to the hand-held end (1 b).
8. The living cell culture apparatus for super resolution imaging according to claim 7, wherein the inner sidewall of the cap body (11) adjacent to the handheld end (1b) is provided with an inclined surface (16), and a normal direction of the inclined surface (16) is provided toward the outside of the cap body (11).
9. The apparatus for culturing living cells for super-resolution imaging according to claim 8, wherein the end face of the handheld end (1b) is provided with a plurality of slots (17) at intervals, and the slots (17) penetrate the inclined plane (16) along the radial direction.
10. A non-destructive transfer module for a living cell culture apparatus for super resolution imaging as defined in any one of claims 1 to 9, characterized in that the non-destructive transfer module has at least one groove (4) for placing a living cell culture apparatus for super resolution imaging, the depth of the groove (4) is smaller than the axial height of a base module (2) of a living cell culture apparatus for super resolution imaging, the inner diameter is slightly larger than the outer diameter of the base module (2), and the bottom surface of the groove (4) is provided with a through hole in the depth direction, the size of which is slightly larger than the imageable area of an imaging slide (3) but smaller than the outer diameter of the base module (2).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120005003.XU CN214218761U (en) | 2021-01-04 | 2021-01-04 | Living cell culture device for super-resolution imaging and lossless transfer module |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120005003.XU CN214218761U (en) | 2021-01-04 | 2021-01-04 | Living cell culture device for super-resolution imaging and lossless transfer module |
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| CN214218761U true CN214218761U (en) | 2021-09-17 |
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| CN202120005003.XU Active CN214218761U (en) | 2021-01-04 | 2021-01-04 | Living cell culture device for super-resolution imaging and lossless transfer module |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A living cell culture device and nondestructive transfer module for super-resolution imaging Effective date of registration: 20220111 Granted publication date: 20210917 Pledgee: Bank of China Limited Guangzhou Development Zone Branch Pledgor: Guangzhou chaoshiji Biotechnology Co.,Ltd. Registration number: Y2022980000324 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |