CN212610538U - Porous culture device for in vitro fertilization - Google Patents

Porous culture device for in vitro fertilization Download PDF

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Publication number
CN212610538U
CN212610538U CN202020756114.XU CN202020756114U CN212610538U CN 212610538 U CN212610538 U CN 212610538U CN 202020756114 U CN202020756114 U CN 202020756114U CN 212610538 U CN212610538 U CN 212610538U
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CN
China
Prior art keywords
culture plate
culture
hole
storage hole
vitro fertilization
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Expired - Fee Related
Application number
CN202020756114.XU
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Chinese (zh)
Inventor
张若鹏
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First Affiliated Hospital Of Dali University
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First Affiliated Hospital Of Dali University
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Priority to CN202020756114.XU priority Critical patent/CN212610538U/en
Application granted granted Critical
Publication of CN212610538U publication Critical patent/CN212610538U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The multi-hole culture device for in vitro fertilization comprises a culture plate, a culture plate and a culture plate fixing seat; the outer diameter of the culture plate is circular; a semen storage hole is arranged in the center of the culture plate; an ovum storage hole is arranged around the semen storage hole; a plurality of ovum storage holes are arranged around the semen storage hole; the top of the culture plate is provided with a culture plate; the bottom of the culture plate is provided with a culture plate fixing seat. The utility model provides stability when high experiment operation and save the sample prevents to pollute between the sample and reveals, and the access is convenient.

Description

Porous culture device for in vitro fertilization
Technical Field
The utility model relates to the technical field of medical culture equipment, concretely relates to porous culture apparatus is used in external fertilization.
Background
One of the more critical core technologies in assisted reproduction is in vitro fertilization. At present, the main implementation methods of the in vitro fertilization technology include 3: 1. the method adopts a micro-drop fertilization mode, namely, a proper amount of sperms are respectively added into a plurality of insemination fluid micro-drops which are prepared in advance, and then, the ovum is put into the insemination fluid micro-drops, thereby achieving the purpose of in vitro fertilization; 2. the hole dish fertilization, namely, a proper amount of sperms are respectively added into the hole dish which is placed with a proper amount of insemination liquid in advance, and then the ovum is placed into the hole dish, so as to achieve the purpose of in vitro fertilization; 3. in the process of in vitro fertilization, a proper amount of sperms are added into a test tube in which a proper amount of insemination liquid is placed in advance, and then ova are placed into the test tube, so that the aim of in vitro fertilization is fulfilled.
When in vitro fertilization and relevant operation, the stability needs to be ensured, the sample can be damaged by unintentional collision and shaking, the experimental operation is influenced, the existing culture vessel for in vitro fertilization is light and convenient, but the stability is poor when the culture vessel is in relatively tight operation, and therefore, the stability during the experimental operation and the sample preservation is improved, and the culture vessel has certain practical significance.
Disclosure of Invention
The utility model aims to avoid the defects of the prior art and provide a porous culture device for in vitro fertilization, thereby effectively solving the problems of the prior art.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the multi-hole culture device for in vitro fertilization comprises a culture plate, a culture plate and a culture plate fixing seat; the outer diameter of the culture plate is circular; a semen storage hole is arranged in the center of the culture plate; an ovum storage hole is arranged around the semen storage hole; a plurality of ovum storage holes are arranged around the semen storage hole; the top of the culture plate is provided with a culture plate; the bottom of the culture plate is provided with a culture plate fixing seat.
Further, a culture plate shell is arranged outside the culture plate; the shell of the culture plate is provided with a cylinder; the top of the culture plate shell is provided with a culture hole fixing sheet; the culture hole fixing piece is fixedly arranged above the culture plate shell; the culture hole fixing sheet is provided with a through hole; the semen storage hole and the ovum storage hole are arranged into cylindrical cup-shaped structures; the activity interlude sets up in the through-hole, and the bottom sprag sets up in the culture plate shell bottom.
Further, the upper edges of the semen storage hole and the ovum storage hole are higher than the shell of the culture plate; and the tops of the semen storage hole and the ovum storage hole are provided with sealing covers.
Furthermore, the sealing cover is in rotary connection with the semen storage hole and the upper edge of the ovum storage hole in a screw structure.
Furthermore, an annular fixing ring is arranged on the outer wall of the culture plate shell; the culture plate fixing seat is arranged into a cylindrical cup-shaped structure, and is sleeved below the culture plate shell; the annular fixing ring is arranged in contact with the top edge of the culture plate fixing seat; the top edge of the culture plate fixing seat is provided with an elastic sealing gasket.
Further, the side wall of the culture plate fixing seat is provided with an air hole; the air hole is connected with the air pump through an air pipe; the air pipe is provided with a valve.
Furthermore, the bottom of the culture plate fixing seat is provided with a weight increasing block.
Compared with the prior art, the utility model discloses following beneficial effect has at least:
the multi-hole culture device for in vitro fertilization designed by the utility model is movable and detachable, and is convenient to take; in addition, through setting up the culture plate fixing base, stability when having improved experiment operation and save the sample prevents to pollute between the sample and reveals, and the access is convenient.
Drawings
Fig. 1 is a schematic sectional structure of the present invention;
FIG. 2 is a schematic top view of the fixing plate of the culture hole of the present invention;
FIG. 3 is a schematic sectional view of the culture plate of the present invention;
in the figure: a culture plate 1; a culture plate cover 2; a culture plate fixing seat 3; a semen storage hole 4; an ovum storage hole 5; a plate housing 6; a culture hole fixing piece 7; a through hole 8; an annular retainer ring 9; an air hole 10; an air pipe 11; an air pump 12; a valve 13; a weight increasing block 14; a cover 15; an elastic seal 16.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
As shown in fig. 1-3:
example 1:
the multi-hole culture device for in vitro fertilization is characterized by comprising a culture plate 1, a culture plate 2 and a culture plate fixing seat 3; the outer diameter of the culture plate 1 is circular; a semen storage hole 4 is arranged in the center of the culture plate 1; an ovum storage hole 5 is arranged around the semen storage hole 4; a plurality of ovum storage holes 5 are arranged around the semen storage hole 4; the top of the culture plate 1 is provided with a culture plate 2; the bottom of the culture plate 1 is provided with a culture plate fixing seat 3. A plurality of ovum storing holes 5 are arranged and are suitable for being used for contrast experiments.
Example 2:
on the basis of example 1, the culture plate 1 is externally provided with a culture plate housing 6; the culture plate shell 6 is provided with a cylinder shape; the top of the culture plate shell 6 is provided with a culture hole fixing sheet 7; the culture hole fixing piece 7 is fixedly arranged above the culture plate shell 6; the culture hole fixing piece 7 is provided with a through hole 8; the semen storage hole 4 and the ovum storage hole 5 are arranged into cylindrical cup-shaped structures; the activity interlude sets up at through-hole 8, and the bottom sprag sets up in the bottom of cultivateing plate shell 6. The utility model discloses a semen storage hole 4 and ovum storage hole 5 all set up to the form that can dismantle the change, and the activity alternates to set up in through-hole 8, conveniently takes the change, when taking place single experiment operation mistake, can change corresponding semen storage hole 4 and ovum storage hole 5, and do not need whole change culture plate 1, it is convenient to bring for the experiment operation.
Example 3:
on the basis of the example 1-2, the upper edges of the semen storage hole 4 and the ovum storage hole 5 are higher than the culture plate housing 6; the top of the semen storage hole 4 and the ovum storage hole 5 is provided with a sealing cover 15. The cover 15 acts as a seal to prevent contamination by liquid spills between the holes.
Example 4:
on the basis of the embodiments 1-3, the cover 15 is rotatably connected with the upper edges of the semen storage hole 4 and the ovum storage hole 5 in a screw structure.
Example 5:
on the basis of the embodiments 1-4, the outer wall of the culture plate shell 6 is provided with an annular fixing ring 9; the culture plate fixing seat 3 is arranged into a cylindrical cup-shaped structure, and the culture plate fixing seat 3 is sleeved below the culture plate shell 6; the annular fixing ring 9 is arranged in contact with the top edge of the culture plate fixing seat 3; the top edge of the culture plate fixing seat 3 is provided with an elastic sealing gasket 16. During experimental operation, in order to keep stable, place culture plate housing 6 below in culture plate fixing base 3 top, the frictional resistance of elastic sealing pad 16 makes it be difficult for removing, avoids the collision, influences the experiment.
Example 6:
on the basis of the embodiments 1 to 5, the side wall of the culture plate fixing seat 3 is provided with an air hole 10; the air hole 10 is connected with an air pump 12 through an air pipe 11; the air pipe is provided with a valve 13. In order to further fix the culture plate 1, the air in the culture plate fixing base 3 is drawn out, and the air is tightly contacted with the culture plate 1, so that the air and the culture plate 1 do not move mutually.
Example 7:
in addition to the embodiments 1-6, the weight-increasing body 14 is provided on the bottom of the plate holder 3. The weight of the culture plate fixing seat 3 can be further increased by adding the weight increasing block, and the fixing effect is enhanced.
The multi-hole culture device for in vitro fertilization designed by the utility model is movable and detachable, and is convenient to take; in addition, through setting up the culture plate fixing base, stability when having improved experiment operation and save the sample prevents to pollute between the sample and reveals, and the access is convenient.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims (7)

1. The multi-hole culture device for in vitro fertilization is characterized by comprising a culture plate (1), a culture plate cover (2) and a culture plate fixing seat (3); the outer diameter of the culture plate (1) is round; a semen storage hole (4) is arranged in the center of the culture plate (1); an ovum storage hole (5) is arranged around the semen storage hole (4); a plurality of ovum storage holes (5) are arranged around the semen storage hole (4); the top of the culture plate (1) is provided with a culture plate cover (2); the bottom of the culture plate (1) is provided with a culture plate fixing seat (3).
2. The multi-well culture device for in vitro fertilization according to claim 1, wherein the culture plate (1) is externally provided with a culture plate housing (6); the culture plate shell (6) is provided with a cylinder shape; the top of the culture plate shell (6) is provided with a culture hole fixing piece (7); the culture hole fixing piece (7) is fixedly arranged above the culture plate shell (6); the culture hole fixing piece (7) is provided with a through hole (8); the semen storage hole (4) and the ovum storage hole (5) are arranged into cylindrical cup-shaped structures; the activity interlude sets up in through-hole (8), and the bottom sprag sets up in culture plate shell (6) bottom.
3. The multi-well culture device for in vitro fertilization according to claim 2, wherein the upper edges of the semen storage hole (4) and the ovum storage hole (5) are higher than the culture plate housing (6); the top parts of the semen storage hole (4) and the ovum storage hole (5) are provided with sealing covers (15).
4. The multi-well culture device for in vitro fertilization according to claim 3, wherein the cap (15) is rotatably connected to the upper edges of the semen storage well (4) and the ovum storage well (5) in a screw structure.
5. The multi-well culture device for in vitro fertilization according to claim 3, wherein the outer wall of the culture plate housing (6) is provided with an annular retainer ring (9); the culture plate fixing seat (3) is of a cylindrical cup-shaped structure, and the culture plate fixing seat (3) is sleeved below the culture plate shell (6); the annular fixing ring (9) is arranged in contact with the top edge of the culture plate fixing seat (3); the top edge of the culture plate fixing seat (3) is provided with an elastic sealing gasket (16).
6. The multi-well culture apparatus for in vitro fertilization according to claim 5, wherein the side wall of the culture plate holder (3) is provided with an air hole (10); the air hole (10) is connected with an air pump (12) through an air pipe (11); the air pipe is provided with a valve (13).
7. The multi-well culture apparatus for in vitro fertilization according to claim 6, wherein the bottom of the plate holder (3) is provided with a weight increasing block (14).
CN202020756114.XU 2020-05-09 2020-05-09 Porous culture device for in vitro fertilization Expired - Fee Related CN212610538U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202020756114.XU CN212610538U (en) 2020-05-09 2020-05-09 Porous culture device for in vitro fertilization

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202020756114.XU CN212610538U (en) 2020-05-09 2020-05-09 Porous culture device for in vitro fertilization

Publications (1)

Publication Number Publication Date
CN212610538U true CN212610538U (en) 2021-02-26

Family

ID=74726327

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202020756114.XU Expired - Fee Related CN212610538U (en) 2020-05-09 2020-05-09 Porous culture device for in vitro fertilization

Country Status (1)

Country Link
CN (1) CN212610538U (en)

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20210226

CF01 Termination of patent right due to non-payment of annual fee