CN219907631U - Dead cell centrifugation device in cell culture solution - Google Patents
Dead cell centrifugation device in cell culture solution Download PDFInfo
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- CN219907631U CN219907631U CN202320881450.0U CN202320881450U CN219907631U CN 219907631 U CN219907631 U CN 219907631U CN 202320881450 U CN202320881450 U CN 202320881450U CN 219907631 U CN219907631 U CN 219907631U
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- block
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- arc
- cell culture
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- 238000005119 centrifugation Methods 0.000 title claims abstract description 13
- 238000004113 cell culture Methods 0.000 title claims abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 58
- 230000006835 compression Effects 0.000 claims description 13
- 238000007906 compression Methods 0.000 claims description 13
- 239000012930 cell culture fluid Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 15
- 239000001963 growth medium Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 210000001589 microsome Anatomy 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a dead cell centrifugation device in a cell culture solution, which comprises a barrel body, wherein a fixed block is arranged in the barrel body, an installation seat is arranged on the upper surface of the fixed block, a fixed assembly is arranged in the installation seat, the fixed assembly comprises a placing groove, a telescopic sleeve, a reagent supporting plate, a buffer spring, a damper and an arc plate, the placing groove is formed in the installation seat, the outer end face of the reagent supporting plate is slidably connected with the inner wall face of the placing groove, two ends of the buffer spring are fixedly connected between the reagent supporting plate and the placing groove, and two ends of the damper are fixedly connected between the reagent supporting plate and the placing groove. According to the utility model, through the arranged structure, the tube body of the reagent tube is clamped and fixed, so that the stability of the reagent tube is ensured, reagent tubes with different sizes can be conveniently fixed, and the reagent tube can be conveniently put into or taken out from the placing groove.
Description
Technical Field
The utility model relates to the technical field of cells, in particular to a dead cell centrifugal device in a cell culture solution.
Background
The growth of cells requires a certain nutrient environment, and a nutrient medium for maintaining the growth of the cells is called a culture medium, namely, all substances for in vitro culture and preservation of the cells for various purposes are named as nutrient environments for artificial simulation of in-vivo growth in the sense, so that the cells have the capability of growing and propagating in the environment, and in scientific cell culture experiments, the culture medium is sold or prepared in a large volume, but because the culture medium is used for one time or a small amount of liquid change, only a small amount of culture medium is needed to be used each time, but some reasons cause cell death, and a centrifugal device is needed to separate the cells; cell centrifugation is a fundamental means of studying, for example, nuclei, mitochondria, golgi, lysosomes and microsomes, and various macromolecules, and utilizes centrifugal force when an object rotates at high speed to cause suspended particles placed in a rotating body to settle or float, thereby achieving the purpose of concentrating or separating certain particles from other particles.
Aiming at the prior patent CN202222502018.4, the device is internally provided with a threaded rod which is matched with an annular block, the annular block can be pressed down to lock the device, the cross block is arranged to prevent hand sliding when screwing, and the effect of convenient screwing is achieved, but the stability of a reagent tube is poor when the reagent tube is placed, and meanwhile, only test tubes of one type can be placed, so that the limitation is larger, the practicability is not high, and the cell centrifugation working efficiency is reduced; therefore, the existing demand is not met, and for this purpose we propose a dead cell centrifuge in cell culture broth.
Disclosure of Invention
The utility model aims to provide a dead cell centrifugal device in a cell culture solution, which aims at solving the problems that aiming at the prior patent CN202222502018.4, the inside of the device of the pathological cell centrifugal device is matched with an annular block through a threaded rod, the annular block can be pressed down to lock the device, the effect of convenient screwing is achieved by preventing hand sliding when screwing through the arrangement of a cross block, but the stability of a reagent tube is poor when the reagent tube is placed, meanwhile, only one type of test tube can be placed, the limitation is larger, the practicability is not high, and the cell centrifugal work efficiency is reduced.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a dead cell centrifugal device in cell culture fluid, includes the ladle body, the inside of ladle body is equipped with the fixed block, the upper surface of fixed block is provided with the mount pad, the internally mounted of mount pad has fixed subassembly, fixed subassembly includes standing groove, telescopic tube, reagent layer board, buffer spring, attenuator and arc, the standing groove has been seted up to the inside of mount pad, the outer terminal surface sliding connection of reagent layer board is in the internal face of standing groove, buffer spring's both ends fixed connection reagent layer board with between the standing groove, the both ends fixed connection of attenuator be in reagent layer board with between the standing groove, the outer terminal surface fixed connection of arc is in the internal face of standing groove, fixed subassembly still includes compression spring, grip block and telescopic tube, compression spring's both ends fixed connection be in the arc with between the grip block, the shape of grip block is the arc, telescopic tube sets up at compression spring's inside wall, just telescopic both ends fixed connection be in between the arc with the grip block.
Preferably, the top of ladle body is equipped with the bung, the inside annular of bung is fixed and is equipped with the rubber ring, one side of mount pad all is equipped with the button, the fixed motor that is equipped with in bottom of fixed block.
Preferably, a barrel cover is arranged on one side of the top of the barrel body through a hinge structure.
Preferably, the number of the installation seats is nine, wherein eight installation seats are installed at the top of the fixed block in an annular array, and the other installation seat is installed at the center of the top of the fixed block.
Preferably, nine holes are formed in the top of the fixing block, and the nine holes are matched with the mounting seat.
Preferably, the button comprises a button block, a connecting rod, a movable block and an arc-shaped block, wherein the connecting rod is arranged on the lower surface of the button block, and the movable block is arranged on the lower surface of the connecting rod.
Preferably, an arc-shaped block is arranged in the middle of the lower surface of the movable block, the bottom end of the arc-shaped block is fixedly connected in a hole formed in the top of the fixed block, and one end of the movable block is fixedly connected with the bottom end of the mounting seat.
Compared with the prior art, the utility model has the beneficial effects that:
1. according to the utility model, the clamping plate is extruded through the reagent tube, and the telescopic sleeve of the inner wall surface of the arc-shaped plate is extruded by the clamping plate to drive the compression spring to deform and generate elasticity, so that the inner wall surface of the clamping plate is clung to the outer end surface of the reagent tube, and the tube body of the reagent tube is clamped and fixed, thereby ensuring the stability of the reagent tube, facilitating the fixation of reagent tubes with different sizes, facilitating the placement or the removal of the reagent tube from the placement groove, and the inner wall surface of the clamping plate is provided with the elastic protection pad, so that the outer end surface of the reagent tube can be protected, buffering can be performed between the clamping plate and the reagent tube, the reagent tube is prevented from being broken, the fixation of reagent tubes with different specifications is realized, and the problems that only one type of test tube can be placed in the prior art and the limitation is large are avoided.
Drawings
FIG. 1 is a schematic view of the overall partial structure of the present utility model;
FIG. 2 is a front view of the entire present utility model;
FIG. 3 is an enlarged partial schematic view of the structure of FIG. 1A according to the present utility model;
fig. 4 is a schematic view showing a partial structure of the push button of the present utility model.
In the figure: 1. a tub body; 2. a barrel cover; 3. a rubber ring; 4. a fixed block; 5. a motor; 6. a mounting base; 7. a button; 71. a button block; 72. a connecting rod; 73. a movable block; 74. an arc-shaped block; 8. a fixing assembly; 81. a placement groove; 82. a telescoping tube; 83. a reagent pallet; 84. a buffer spring; 85. a damper; 86. an arc-shaped plate; 861. a compression spring; 862. and a clamping plate.
Description of the embodiments
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
Referring to fig. 1 to 4, an embodiment of the present utility model provides: the utility model provides a dead cell centrifugal device in cell culture liquid, including ladle body 1, the inside of ladle body 1 is equipped with fixed block 4, the upper surface of fixed block 4 is provided with mount pad 6, the internally mounted of mount pad 6 has fixed subassembly 8, fixed subassembly 8 includes standing groove 81, telescopic tube 82, reagent layer board 83, buffer spring 84, attenuator 85 and arc 86, standing groove 81 has been seted up to the inside of mount pad 6, reagent layer board 83's outer terminal surface sliding connection is at the internal face of standing groove 81, buffer spring 84's both ends fixed connection is between reagent layer board 83 and standing groove 81, the both ends fixed connection of attenuator 85 is between reagent layer board 83 and standing groove 81, the external terminal surface fixed connection of arc 86 is at the internal face of standing groove 81, fixed subassembly 8 still includes compression spring 861, clamping plate 862 and telescopic tube 82, compression spring 861's both ends fixed connection is between arc 86 and clamping plate 862, the shape of clamping plate 862 is the arc, telescopic tube 82 sets up at compression spring 861's inside wall, and telescopic tube 82's both ends fixed connection is between arc 86 and clamping plate 862.
Further, the top of ladle body 1 is equipped with bung 2, and the inside annular of bung 2 is fixed to be equipped with rubber ring 3, and one side of mount pad 6 all is equipped with button 7, and fixed motor 5 that is equipped with in the bottom of fixed block 4.
Through adopting above-mentioned technical scheme, through the rubber ring 3 that is provided with for when bung 2 is closed, with the leakproofness of ladle body 1 strong.
Further, a barrel cover 2 is arranged on one side of the top of the barrel body 1 through a hinge structure.
Further, the number of the mounting seats 6 is nine, wherein eight mounting seats 6 are installed at the top of the fixed block 4 in an annular array, and another mounting seat 6 is installed at the top center of the fixed block 4.
Further, nine holes are formed in the top of the fixing block 4, and the nine holes are matched with the mounting seat 6.
Further, the button 7 includes a button block 71, a connection rod 72, a movable block 73, and an arc block 74, the lower surface of the button block 71 is provided with the connection rod 72, and the lower surface of the connection rod 72 is provided with the movable block 73.
Further, an arc block 74 is arranged in the middle of the lower surface of the movable block 73, the bottom end of the arc block 74 is fixedly connected in a hole formed in the top of the fixed block 4, and one end of the movable block 73 is fixedly connected with the bottom end of the mounting seat 6.
Through adopting above-mentioned technical scheme, through the cooperation of button piece 71 and movable block 73, can jack up mount pad 6, reach the effect of conveniently taking.
Working principle: during the use, through aiming at the top of standing groove 81 with the reagent pipe, press the top of reagent pipe and inject it into the inside of standing groove 81, make the bottom card of reagent pipe in the recess of the inside seting up of reagent layer board 83, in this period, carry out the centre gripping to the body of reagent pipe through arc 86 and fix, thereby guarantee the stability of reagent pipe, buffer the impact force in the transportation reagent pipe centrifugation process through buffer spring 84, and reduce the amplitude that produces because of rocking by damper 85, thereby guarantee the stability of reagent pipe, facilitate the use, the recess of seting up in reagent layer board 83 inside is the arc, can increase the area of contact with the reagent pipe, make it hug closely the outer terminal surface of reagent pipe, be convenient for operating personnel get it, along with the inside of reagent pipe intubate standing groove 81, extrude grip plate 862 through the reagent pipe, the telescopic tube 82 of grip plate 862 inner wall surface drives compression spring 861 and takes place the deformation and produce elasticity, thereby make the inner wall surface of grip plate 862 hug closely the outer terminal surface of reagent pipe, thereby carry out the centre gripping to the body of reagent pipe and fix, thereby guarantee the stability of reagent pipe, be convenient for carrying out the stability of reagent pipe, be convenient for carry out the size to the reagent pipe and fix the size, and keep out the reagent pipe from the inside the grip plate 862, can also be put into the reagent pipe into the buffer plate 862, can be protected with the reagent pad is protected from the inside the buffer plate is protected by the reagent pad is placed to the inside the reagent pipe.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (7)
1. The utility model provides a dead cell centrifugation device in cell culture liquid, includes ladle body (1), its characterized in that: the inside of ladle body (1) is equipped with fixed block (4), the upper surface of fixed block (4) is provided with mount pad (6), the internally mounted of mount pad (6) has fixed subassembly (8), fixed subassembly (8) are including standing groove (81), telescopic tube (82), reagent layer board (83), buffer spring (84), attenuator (85) and arc (86), standing groove (81) have been seted up to the inside of mount pad (6), the outer terminal surface sliding connection of reagent layer board (83) is in the internal face of standing groove (81), the both ends fixed connection of buffer spring (84) are in reagent layer board (83) with between standing groove (81), the both ends fixed connection of attenuator (85) are in between reagent layer board (83) and the standing groove (81), the outer terminal surface fixed connection of arc (86) is in the internal face of standing groove (81), fixed subassembly (8) still include compression spring (861), grip block (862) and sleeve (82), compression spring (86) are in between the both ends fixed connection of arc (861) compression spring (862) are in the compression sleeve (861), and the two ends of the telescopic sleeve (82) are fixedly connected between the arc-shaped plate (86) and the clamping plate (862).
2. The dead cell centrifugation device in a cell culture fluid according to claim 1, wherein: the top of ladle body (1) is equipped with bung (2), the inside annular of bung (2) is fixed and is equipped with rubber ring (3), one side of mount pad (6) all is equipped with button (7), the bottom of fixed block (4) is fixed and is equipped with motor (5).
3. The dead cell centrifugation device in a cell culture fluid according to claim 1, wherein: a barrel cover (2) is arranged on one side of the top of the barrel body (1) through a hinge structure.
4. The dead cell centrifugation device in a cell culture fluid according to claim 1, wherein: the number of the mounting seats (6) is nine, wherein eight mounting seats (6) are arranged at the top of the fixed block (4) in an annular array, and the other mounting seat (6) is arranged at the center of the top of the fixed block (4).
5. The dead cell centrifugation device in a cell culture fluid according to claim 1, wherein: nine holes are formed in the top of the fixing block (4), and the nine holes are matched with the mounting seat (6).
6. A dead cell centrifugation device in a cell culture fluid according to claim 2, wherein: the button (7) comprises a button block (71), a connecting rod (72), a movable block (73) and an arc-shaped block (74), wherein the connecting rod (72) is arranged on the lower surface of the button block (71), and the movable block (73) is arranged on the lower surface of the connecting rod (72).
7. The dead cell centrifugation device in a cell culture fluid according to claim 6, wherein: the middle of movable block (73) lower surface is provided with arc piece (74), the bottom fixed connection of arc piece (74) is in fixed block (4) top is equipped with the hole, the one end of movable block (73) with the bottom fixed connection of mount pad (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320881450.0U CN219907631U (en) | 2023-04-19 | 2023-04-19 | Dead cell centrifugation device in cell culture solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320881450.0U CN219907631U (en) | 2023-04-19 | 2023-04-19 | Dead cell centrifugation device in cell culture solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219907631U true CN219907631U (en) | 2023-10-27 |
Family
ID=88439081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320881450.0U Active CN219907631U (en) | 2023-04-19 | 2023-04-19 | Dead cell centrifugation device in cell culture solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219907631U (en) |
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2023
- 2023-04-19 CN CN202320881450.0U patent/CN219907631U/en active Active
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