CN114002839B - Liquid immersion objective capable of being used reversely and microscope with liquid immersion objective - Google Patents
Liquid immersion objective capable of being used reversely and microscope with liquid immersion objective Download PDFInfo
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- CN114002839B CN114002839B CN202111151035.1A CN202111151035A CN114002839B CN 114002839 B CN114002839 B CN 114002839B CN 202111151035 A CN202111151035 A CN 202111151035A CN 114002839 B CN114002839 B CN 114002839B
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- ring
- immersion
- liquid
- dielectric film
- immersion objective
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- 238000007654 immersion Methods 0.000 title claims abstract description 118
- 239000007788 liquid Substances 0.000 title claims abstract description 99
- 238000007789 sealing Methods 0.000 claims abstract description 42
- 238000002347 injection Methods 0.000 claims description 30
- 239000007924 injection Substances 0.000 claims description 30
- 238000003825 pressing Methods 0.000 claims description 12
- 239000013013 elastic material Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000003466 welding Methods 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims 1
- 230000002441 reversible effect Effects 0.000 claims 1
- 230000003287 optical effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 239000004033 plastic Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 2
- 238000007731 hot pressing Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/33—Immersion oils, or microscope systems or objectives for use with immersion fluids
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- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Lenses (AREA)
Abstract
The invention is suitable for the technical field of optical equipment and instruments, and provides an immersion objective lens capable of being used reversely and a microscope with the immersion objective lens. The immersion objective comprises an immersion objective body and a dielectric film arranged at the front end of the immersion objective body, wherein the refractive index of the dielectric film is the same as that of a liquid medium outside the immersion objective body; the immersion liquid objective body is sleeved with an immersion liquid ring, a sealing cavity is formed among the dielectric film, the immersion liquid ring and the immersion liquid objective body, and liquid medium is injected into the sealing cavity. The microscope has the immersion objective described above. According to the liquid immersion objective lens capable of being used reversely and the microscope with the liquid immersion objective lens, when a sample is replaced, only the carrying platform for placing the sample is needed to be directly taken out, the objective lens does not need to be moved, the space above the carrying platform for the sample is enough, the main light path height is reduced, the system stability is superior to that of the normal use, the sample is not directly soaked in a medium, the medium does not need to be replaced frequently, and the use cost of a microscope system is reduced.
Description
Technical Field
The invention belongs to the technical field of optical equipment and instruments, and particularly relates to an immersion objective lens capable of being used reversely and a microscope with the immersion objective lens.
Background
The resolution of optical microscopy imaging depends to a large extent on the numerical aperture of the objective, whereas increasing the refractive index of the medium between the objective and the sample can significantly increase the numerical aperture of the objective, a high refractive index medium typically being an aqueous or oily liquid medium, requiring the addition of a liquid medium between the sample and the objective, i.e. an immersion objective, when in use. The immersion objective requires immersing the front section in a liquid medium and is difficult to invert for application.
The high refractive index medium enables the immersion objective to have a higher numerical aperture, the resolution is improved, and in order to form an effective medium layer between the sample and the medium, the immersion objective is usually used in a positive position, i.e. the sample is immersed in the medium, and the immersion objective is arranged above the sample, so that the front section of the immersion objective is immersed in the medium. In the prior art, when the immersion objective is used in a normal position, the loading of the sample often requires that the objective is moved a long distance at first, which causes a certain difficulty in refocusing, and the distance between the objective and the sample carrier is smaller, so that the size of the sample is limited; in addition, the immersion objective lens is used in a positive way, most of light paths need to be placed above the sample carrier, the overall stability is poor, and extra expenditure is required to be added in the aspect of system stability; the sample is directly soaked in the medium, the medium is required to be replaced when the sample is replaced, and the medium is often expensive, so that the application cost is high.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides an immersion objective lens capable of being used in an inverted mode and a microscope with the immersion objective lens, which can be used in an inverted mode, and are good in use effect and low in application cost.
The technical scheme of the invention is as follows: an immersion objective capable of being used reversely comprises an immersion objective body and a dielectric film arranged at the front end of the immersion objective body, wherein the refractive index of the dielectric film is the same as that of a liquid medium outside the immersion objective body; the immersion liquid objective body is sleeved with an immersion liquid ring, a sealing cavity is formed among the dielectric film, the immersion liquid ring and the immersion liquid objective body, and liquid medium is injected into the sealing cavity.
Optionally, the annotate the liquid ring be connected with be used for with the dielectric film compress tightly in annotate the clamping ring of liquid ring, the dielectric film with annotate and set up first sealing washer between the liquid ring.
Optionally, the periphery of dielectric film is annular protruding edge, annotate the liquid ring and be provided with be used for installing the ring channel of first sealing washer, the bottom surface gland of annular protruding edge in the ring channel and with first sealing washer meets, the clamping ring press in the positive of annular protruding edge and fixed connection in annotate the liquid ring, the clamping ring pass through the retaining member connect in annotate the liquid ring.
Optionally, the dielectric film is hermetically connected to the liquid injection ring by means of hot pressing or ultrasonic welding.
Optionally, the liquid injection ring is provided with a liquid inlet and an air outlet.
Optionally, the immersion objective further includes a positioning ring, where the positioning ring is sleeved on the immersion objective body, and the positioning ring and the immersion objective body are coaxially disposed.
Optionally, the immersion objective further comprises a loading ring, the loading ring is located between the positioning ring and the immersion objective body, the positioning ring is in threaded connection with a positioning piece, and the positioning piece abuts against the loading ring.
Optionally, the immersion objective further comprises a relief ring, the relief ring being located between the loading ring and the immersion objective body.
Optionally, the loading ring is made of a rigid material; the load relief ring is made of elastic materials.
The invention also provides a microscope provided with the immersion objective which can be used reversely.
According to the liquid immersion objective which can be used reversely and the microscope with the liquid immersion objective, provided by the invention, the liquid immersion objective is used reversely, when a sample is replaced, only the carrying platform for placing the sample is needed to be directly taken out, the objective is not needed to be moved, the space above the carrying platform for the sample is enough, the size of the sample is not limited by the sizes of the objective and the carrying platform, the main light path height is reduced, the system stability is superior to that of the normal use, the sample is not directly soaked in a medium, the medium is not needed to be replaced frequently, and the use cost of a microscopic system is reduced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is an exploded perspective view of an immersion objective that may be used upside down according to an embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of an immersion objective that may be used upside down according to an embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
It will be understood that when an element is referred to as being "mounted" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present.
It should be noted that, in the embodiments of the present invention, terms such as left, right, up, and down are merely relative concepts or references to normal use states of the product, and should not be construed as limiting.
As shown in fig. 1 and fig. 2, an immersion objective lens capable of being used in an inverted manner according to an embodiment of the present invention includes an immersion objective lens body 10 and a dielectric film 2 disposed at a front end of the immersion objective lens body 10, wherein the refractive index of the dielectric film 2 is the same as that of a liquid medium outside the immersion objective lens body 10; the immersion objective body 10 is sleeved with an immersion ring 4, a sealing cavity 100 is formed between the dielectric film 2, the immersion ring 4 and the immersion objective body 10, a liquid medium (not shown in the figure) is injected into the sealing cavity 100, an independent sealing cavity 100 is provided at the front section of the immersion objective, the front end surface of the sealing cavity 100 is a dielectric film 2 (which can be a transparent plastic film) with the same refractive index as the liquid medium outside the immersion objective body 10, the liquid medium (including but not limited to oily liquid) required by the immersion objective is filled in the sealing cavity 100, the same high refractive index dielectric layer is formed between a sample and the immersion objective, the independent dielectric layer is provided for the immersion objective, the immersion objective can be used upside down, the loading of the sample can not cause refocusing difficulty, the distance between the objective and the sample carrier can be increased, and the placement of a larger sample is facilitated; in addition, the immersion liquid objective lens can be inverted, the overall stability of the optical path is better, the stability requirement of the system can be correspondingly reduced, namely, extra expenditure on the stability of the system is not required, a medium is not required to be replaced when a sample is replaced, and the application cost is low.
In the specific application, the sample can be born through the glass slide, the immersion objective can be positioned below the glass slide and used in an inverted mode, when the sample is replaced, only the carrier for placing the sample is needed to be directly taken out, the objective is not needed to be moved, the space above the sample carrier is enough, the size of the sample is not limited by the size of the objective and the carrier any more, the height of a main light path is reduced, the stability of the system is superior to that of the normal use, the sample is not directly soaked in a medium, the medium is not needed to be replaced frequently, and the use cost of a microscopic system is reduced.
Specifically, the liquid injection ring 4 may be connected to a pressing ring 1 for pressing the dielectric film 2 against the liquid injection ring 4, and a first sealing ring 3 may be disposed between the dielectric film 2 and the liquid injection ring 4 to prevent leakage of the liquid medium.
Specifically, the periphery of the dielectric film 2 is an annular convex edge 21, and the liquid injection ring 4 is provided with an annular groove 41 for installing the first sealing ring 3. The bottom surface of the annular convex edge 21 is pressed against the annular groove 41 and is connected with the first sealing ring 3, the pressing ring 1 is pressed against the front surface of the annular convex edge 21 and is fixedly connected with the liquid injection ring 4, the first sealing ring 3 can be clamped in the annular groove 41, and the pressing ring 1 presses the annular convex edge 21 and the first sealing ring 3 of the dielectric film 2.
Specifically, a second sealing ring 6 can be arranged between the liquid injection ring 4 and the immersion liquid objective body 10, an annular groove can be formed in the inner side of the liquid injection ring 4 or/and the outer side of the immersion liquid objective body 10, and the second sealing ring 6 can be clamped in the annular groove, so that the sealing effect is good.
Specifically, the pressing ring 1 is connected to the liquid injection ring 4 through a locking member 11, and the locking member 11 can be a bolt, so that the pressing ring is convenient to disassemble and assemble, is favorable for subsequent replacement of the dielectric film 2, and has good locking effect and reliable sealing. Of course, the pressing ring 1 may be connected to the liquid injection ring 4 by a buckle, a screw structure, or the like, and the liquid injection ring 4 may be a metal piece or a hard plastic piece.
Or the pressing ring 1 can be omitted, the liquid injection ring 4 can be a plastic part, the annular convex edge 21 of the dielectric film 2 is in sealing connection with the liquid injection ring 4 in a hot pressing or ultrasonic welding mode, and the sealing connection between the annular convex edge 21 of the dielectric film 2 and the liquid injection ring 4 can also be realized.
Specifically, the liquid injection ring 4 is provided with a liquid inlet 42 and a discharge outlet 43, the liquid inlet 42 may be connected to a liquid inlet pipe through a screw structure or a luer, the discharge outlet 43 may be connected to a discharge pipe through a screw structure or a luer, and the discharge outlet 43 may discharge the gas in the sealed cavity 100. The pressure in the sealed cavity 100 can be adjusted by the pressure adjusting device, so that the tension of the dielectric film 2 is different, and the focal length can be conveniently adjusted.
Specifically, the immersion liquid objective further comprises a positioning ring 5, the positioning ring 5 is fixedly sleeved outside the immersion liquid objective body 10, the positioning ring 5 and the immersion liquid objective body 10 are coaxially arranged, one end of the liquid injection ring 4 is connected with the positioning ring 5, and the other end of the liquid injection ring 4 is connected with the dielectric film 2 and the pressing ring 1. One end of the liquid injection ring 4 can be connected through a threaded structure or a spin-clip structure.
In a specific application, the front end of the immersion objective body 10 is provided with a truncated cone or a truncated cone-shaped bulge, and the dielectric film 2 is correspondingly arranged into a cylinder or cone-shaped structure.
Specifically, the immersion objective further comprises a loading ring 8, the loading ring 8 is located between the positioning ring 5 and the immersion objective body 10, the positioning ring 5 is in threaded connection with a positioning piece 9, and the positioning piece 9 abuts against the loading ring 8 so as to avoid damaging the immersion objective body 10. The positioning pieces 9 can be provided with a plurality of positioning pieces and are uniformly distributed along the circumferential direction of the positioning ring 5 so as to facilitate installation and adjustment. The positioning member 9 may be a positioning screw.
Specifically, the immersion objective further comprises a load shedding ring 7, and the load shedding ring 7 is located between the load shedding ring 8 and the immersion objective body 10, so as to play a role of buffering, and further avoid damaging the immersion objective body 10.
In a specific application, the inner side of the positioning ring 5 may be stepped, including a first inner side wall and a second inner side wall, and the inner diameter of the first inner side wall of the positioning ring 5 is greater than the inner diameter of the second inner side wall. The first inside wall department is provided with the internal thread that is used for being connected with annotating liquid ring 4, annotates the lower terminal surface of liquid ring 4 and can support in the step face between first inside wall and the second inside wall. The load ring 8 is disposed on the inner side of the second inner sidewall, and the load shedding ring 7 is disposed on the inner side of the load ring 8.
In this embodiment, the loading ring 8 is made of a rigid material, such as stainless steel, hard plastic, etc.; the relief ring 7 may be made of an elastic material, such as rubber, silicone, etc.
During installation, the load shedding ring 7 is installed on a proper position on the outer surface of the immersion objective body 10, then the loading ring 8 is installed on the outer surface of the load shedding ring 7, the positioning ring 5 is installed outside the loading ring 8, the positioning screw (the positioning piece 9) is screwed down, fine adjustment is performed, the positioning ring 5 is coaxial with the immersion objective body 10, the liquid injection ring 4 is screwed onto the positioning ring 5, then the first sealing ring 3 is installed on the annular groove 41 on the liquid injection ring 4, the dielectric film 2 is placed on the upper surface of the liquid injection ring 4, the pressing ring 1 is locked on the liquid injection ring 4 through bolts, and the first sealing ring 3 is extruded, so that the stroke between the dielectric film 2 and the liquid injection ring 4 is reliably sealed.
In operation, the outer surfaces of the dielectric film 2, the first sealing ring 3, the liquid injection ring 4, the second sealing ring 6 and the immersion objective body 10 form a sealing cavity 100, liquid medium enters the sealing cavity 100 from the liquid injection hole, and air in the sealing cavity 100 is discharged from the discharge port 43, so that the sealing cavity 100 is filled with the liquid medium.
Specifically, through testing Olympus XLUMPLFLN XW water immersion objective, a 2mm liquid medium layer can be formed between the medium film 2 and the front surface of the immersion objective, and the sample just falls within the working distance of the objective, so that the effect is good.
The embodiment of the invention also provides a microscope, which is provided with the immersion liquid objective lens capable of being used reversely.
According to the immersion objective lens capable of being used reversely and the microscope with the immersion objective lens, provided by the embodiment of the invention, the immersion objective lens is used reversely, when a sample is replaced, only the carrying platform for placing the sample is needed to be directly taken out, the objective lens does not need to be moved, the space above the carrying platform for the sample is enough, the size of the sample is not limited by the sizes of the objective lens and the carrying platform any more, the main light path height is reduced, the stability of the system is superior to that of the normal use, the sample is not directly soaked in a medium, the medium does not need to be replaced frequently, and the use cost of a microscopic system is reduced.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, or alternatives falling within the spirit and principles of the invention.
Claims (9)
1. An immersion objective capable of being used reversely is characterized by comprising an immersion objective body and a dielectric film arranged at the front end of the immersion objective body, wherein the refractive index of the dielectric film is the same as that of a liquid medium outside the immersion objective body; the immersion liquid objective body is sleeved with an immersion liquid ring, the immersion liquid ring is provided with a liquid inlet and a gas outlet, a sealing cavity is formed among the dielectric film, the immersion liquid ring and the immersion liquid objective body, the front end face of the sealing cavity is the dielectric film, a first sealing ring is arranged between the dielectric film and the immersion liquid ring, a second sealing ring is arranged between the immersion liquid ring and the immersion liquid objective body, the immersion liquid ring is in sealing connection with the dielectric film, the sealing cavity is formed by the dielectric film, the first sealing ring, the immersion liquid ring, the second sealing ring and the outer surface of the immersion liquid objective body, liquid medium is injected into the sealing cavity, and liquid medium is arranged between the dielectric film and the front surface of the immersion liquid objective body; the front end of the immersion liquid objective body is provided with a round table or a frustum-shaped bulge, and the dielectric film is of a cylindrical or conical structure.
2. An invertible immersion lens according to claim 1, wherein said immersion ring is connected to a clamping ring for clamping said dielectric film to said immersion ring.
3. An inverted immersion lens according to claim 2, wherein the periphery of the dielectric film is an annular flange, the liquid injection ring is provided with an annular groove for mounting the first sealing ring, the bottom surface of the annular flange is pressed against the annular groove and is connected with the first sealing ring, the pressing ring is pressed against the front surface of the annular flange and is fixedly connected with the liquid injection ring, and the pressing ring is connected with the liquid injection ring through a locking member.
4. An inverted use immersion objective according to claim 1, wherein the dielectric film is sealingly connected to the liquid injection ring by means of thermo-compression or ultrasonic welding.
5. An inverted use immersion objective according to any one of claims 1 to 4, further comprising a retaining ring, the retaining ring being arranged around the immersion objective body and being arranged coaxially with the immersion objective body.
6. An inverted use immersion objective as claimed in claim 5, further comprising a loading ring between the positioning ring and the immersion objective body, the positioning ring being threadably connected with a positioning member that abuts the loading ring.
7. An invertible immersion lens according to claim 6, further comprising a de-load ring disposed between said load ring and said immersion lens body.
8. An inverted use immersion lens as claimed in claim 7, wherein said load ring is made of a rigid material; the load relief ring is made of elastic materials.
9. A microscope having an immersion objective as claimed in any one of claims 1 to 8 which is reversible.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111151035.1A CN114002839B (en) | 2021-09-29 | 2021-09-29 | Liquid immersion objective capable of being used reversely and microscope with liquid immersion objective |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111151035.1A CN114002839B (en) | 2021-09-29 | 2021-09-29 | Liquid immersion objective capable of being used reversely and microscope with liquid immersion objective |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114002839A CN114002839A (en) | 2022-02-01 |
| CN114002839B true CN114002839B (en) | 2024-06-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111151035.1A Active CN114002839B (en) | 2021-09-29 | 2021-09-29 | Liquid immersion objective capable of being used reversely and microscope with liquid immersion objective |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001091849A (en) * | 1999-09-21 | 2001-04-06 | Olympus Optical Co Ltd | Liquid immersion objective lens for microscope |
| CN203799108U (en) * | 2014-04-24 | 2014-08-27 | 中国人民解放军第三军医大学第一附属医院 | Microscope objective lens bath conversion device |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1717628B1 (en) * | 2004-02-16 | 2012-01-25 | Olympus Corporation | Immersion objective lens, retention mechanism for immersion medium and manufacturing method thereof |
| JP2008197580A (en) * | 2007-02-15 | 2008-08-28 | Tokai Hit:Kk | Objective lens warming device |
| CN102385078B (en) * | 2011-10-25 | 2016-06-29 | 洛阳师范学院 | A kind of remote-controlled continuous zoom lens with liquid |
| JP6261357B2 (en) * | 2014-01-30 | 2018-01-17 | オリンパス株式会社 | Microscope and observation method |
| JP2018146810A (en) * | 2017-03-07 | 2018-09-20 | オリンパス株式会社 | Optical system, immersion liquid holder and observation device |
| DE102019108611B3 (en) * | 2019-04-02 | 2020-08-06 | Leica Microsystems Cms Gmbh | Device and method for feeding an immersion medium and objective with a feeding device |
| CN112650028B (en) * | 2020-12-25 | 2024-02-09 | 浙江启尔机电技术有限公司 | Immersion liquid supply recovery device for improving pressure characteristic of immersion flow field |
-
2021
- 2021-09-29 CN CN202111151035.1A patent/CN114002839B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001091849A (en) * | 1999-09-21 | 2001-04-06 | Olympus Optical Co Ltd | Liquid immersion objective lens for microscope |
| CN203799108U (en) * | 2014-04-24 | 2014-08-27 | 中国人民解放军第三军医大学第一附属医院 | Microscope objective lens bath conversion device |
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| CN114002839A (en) | 2022-02-01 |
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