CN114002839A - Immersion objective lens capable of being used reversely and microscope with immersion objective lens - Google Patents
Immersion objective lens capable of being used reversely and microscope with immersion objective lens Download PDFInfo
- Publication number
- CN114002839A CN114002839A CN202111151035.1A CN202111151035A CN114002839A CN 114002839 A CN114002839 A CN 114002839A CN 202111151035 A CN202111151035 A CN 202111151035A CN 114002839 A CN114002839 A CN 114002839A
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- immersion objective
- ring
- objective lens
- dielectric film
- immersion
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- 238000007654 immersion Methods 0.000 title claims abstract description 101
- 239000007788 liquid Substances 0.000 claims abstract description 69
- 238000002347 injection Methods 0.000 claims abstract description 39
- 239000007924 injection Substances 0.000 claims abstract description 39
- 238000007789 sealing Methods 0.000 claims abstract description 25
- 238000003825 pressing Methods 0.000 claims description 15
- 239000013013 elastic material Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000003466 welding Methods 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 abstract description 7
- 229920003023 plastic Polymers 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000007731 hot pressing Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/33—Immersion oils, or microscope systems or objectives for use with immersion fluids
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- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Lenses (AREA)
Abstract
The invention is applicable to the technical field of optical equipment instruments, and provides an immersion objective lens capable of being used reversely and a microscope with the immersion objective lens. The immersion objective comprises an immersion objective body and a dielectric film arranged at the front end of the immersion objective body, wherein the refractive index of the dielectric film is the same as that of a liquid medium outside the immersion objective body; the immersion objective body is sleeved with an injection ring, a sealing cavity is formed between the dielectric film, the injection ring and the immersion objective body, and a liquid medium is injected into the sealing cavity. The microscope has the immersion objective lens. The immersion objective lens capable of being used in an inverted mode and the microscope with the immersion objective lens are used in an inverted mode, when a sample is replaced, only the carrying platform for placing the sample needs to be taken out directly, the objective lens does not need to be moved, the space above the sample carrying platform is enough, the height of a main optical path is reduced, the stability of the system is superior to that of the immersion objective lens used in an upright mode, the sample is not directly soaked in a medium, the medium does not need to be replaced frequently, and the use cost of the microscope system is reduced.
Description
Technical Field
The invention belongs to the technical field of optical equipment instruments, and particularly relates to an immersion objective lens capable of being used in an inverted mode and a microscope with the immersion objective lens.
Background
The resolution of optical microscopy imaging depends to a large extent on the numerical aperture of the objective lens, while increasing the refractive index of the medium between the objective lens and the sample can significantly increase the numerical aperture of the objective lens, and high refractive index media are typically aqueous or oily liquid media, and require the addition of a liquid medium between the sample and the objective lens during use, i.e. immersion objectives. Immersion objectives require the front section to be immersed in a liquid medium and are difficult to use upside down.
The high refractive index medium enables the immersion objective to have a higher numerical aperture, improves the resolution, and normally enables the immersion objective to be used in a positive mode in order to form an effective medium layer between a sample and the medium, namely, the sample is immersed in the medium, and the immersion objective is placed above the sample, so that the front section of the immersion objective is immersed in the medium. In the prior art, when the immersion objective is used in a positive mode, the sample is loaded, the objective is often required to be moved for a long distance, certain difficulty is caused for refocusing, and the distance between the objective and the sample carrying platform is small, so that the size of the sample is limited; moreover, the immersion objective is used in a positive mode, most of the optical path needs to be placed above the sample carrying platform, the overall stability is poor, and extra expenditure needs to be added in the aspect of the stability of the system; the sample is directly soaked in the medium, and the medium needs to be replaced when the sample is replaced, and the medium is expensive and high in application cost.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides an immersion objective lens capable of being used in an inverted mode and a microscope with the immersion objective lens, wherein the immersion objective lens can be used in an inverted mode, the using effect is good, and the application cost is low.
The technical scheme of the invention is as follows: an immersion objective lens capable of being used in an inverted mode comprises an immersion objective lens body and a dielectric film arranged at the front end of the immersion objective lens body, wherein the refractive index of the dielectric film is the same as that of a liquid medium outside the immersion objective lens body; the immersion objective body is sleeved with a liquid injection ring, a sealing cavity is formed between the dielectric film, the liquid injection ring and the immersion objective body, and a liquid medium is injected into the sealing cavity.
Optionally, the liquid injection ring is connected with a pressing ring for pressing the dielectric film against the liquid injection ring, and a first sealing ring is arranged between the dielectric film and the liquid injection ring.
Optionally, the periphery of the dielectric film is an annular convex edge, the liquid injection ring is provided with an annular groove for installing the first sealing ring, the bottom surface of the annular convex edge is pressed against the annular groove and connected with the first sealing ring, the pressing ring is pressed against the front surface of the annular convex edge and fixedly connected to the liquid injection ring, and the pressing ring is connected to the liquid injection ring through a locking piece.
Optionally, the dielectric film is hermetically connected to the liquid injection ring by means of hot pressing or ultrasonic welding.
Optionally, the liquid injection ring is provided with a liquid inlet and an air outlet.
Optionally, the immersion objective further comprises a positioning ring, the positioning ring is sleeved on the immersion objective body, and the positioning ring and the immersion objective body are coaxially arranged.
Optionally, the immersion objective further comprises a loading ring, the loading ring is located between the positioning ring and the immersion objective body, the positioning ring is in threaded connection with a positioning piece, and the positioning piece abuts against the loading ring.
Optionally, the immersion objective further comprises a load relief ring located between the load ring and the immersion objective body.
Optionally, the loading ring is made of a rigid material; the load relief ring is made of elastic materials.
The invention also provides a microscope having an invertible immersion objective as described above.
The immersion objective lens capable of being used in an inverted mode and the microscope with the immersion objective lens are used in an inverted mode, when a sample is replaced, only the carrying platform for placing the sample needs to be taken out directly, the objective lens does not need to be moved, the space above the sample carrying platform is enough, the size of the sample is not limited by the size of the objective lens and the size of the carrying platform, the height of a main optical path is reduced, the stability of the system is superior to that of the system used in an upright mode, the sample is not directly soaked in a medium, the medium does not need to be replaced frequently, and the use cost of the microscope system is reduced.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is an exploded perspective view of an invertible immersion objective according to an embodiment of the present invention;
fig. 2 is a schematic cross-sectional view of an immersion objective lens that can be used upside down according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present.
It should be noted that the terms of orientation such as left, right, up and down in the embodiments of the present invention are only relative to each other or are referred to the normal use state of the product, and should not be considered as limiting.
As shown in fig. 1 and fig. 2, an immersion objective lens capable of being used upside down according to an embodiment of the present invention includes an immersion objective lens body 10 and a dielectric film 2 disposed at a front end of the immersion lens body 10, wherein the dielectric film 2 has the same refractive index as a liquid medium outside the immersion lens body 10; the immersion objective body 10 is sleeved with an injection ring 4, a sealing cavity 100 is formed between the dielectric film 2, the injection ring 4 and the immersion objective body 10, the chamber 100 is filled with a liquid medium (not shown), by providing a separate chamber 100 in the front section of the immersion objective, and the front end face of the sealed chamber 100 is a dielectric film 2 (which may be a transparent plastic film) having the same refractive index as the liquid medium outside the immersion objective body 10, the chamber 100 is filled with a liquid medium (including but not limited to an oily liquid) required for the immersion objective, with the same high refractive index medium layer between the sample and the immersion objective, providing a separate medium layer for the immersion objective, the immersion objective can be used reversely, the sample loading does not cause difficulty in refocusing, and the distance between the objective and the sample carrier can be increased, so that a larger sample can be placed conveniently; moreover, the immersion objective can be inverted, the overall stability of the optical path is better, the requirement on the stability of the system can be correspondingly reduced, namely, extra expenditure on the stability of the system is not required, a medium is not required to be replaced when a sample is replaced, and the application cost is low.
In the concrete application, the sample can bear through the slide glass, and immersion liquid objective can be located the below of slide glass and invert the use, when changing the sample, only need directly take out the microscope carrier that places the sample, need not remove objective, and the space of sample microscope carrier top is enough, and the sample size no longer receives the restriction of objective and microscope carrier size, and main light path height reduces, and system stability is superior to just putting the use, and the sample does not directly soak within the medium, need not frequently change the medium, reduces microsystem use cost.
Specifically, the liquid injection ring 4 may be connected with a pressing ring 1 for pressing the dielectric film 2 to the liquid injection ring 4, and a first sealing ring 3 may be disposed between the dielectric film 2 and the liquid injection ring 4 to prevent leakage of the liquid medium.
Specifically, the periphery of the dielectric film 2 is an annular convex edge 21, and the liquid injection ring 4 is provided with an annular groove 41 for mounting the first sealing ring 3. The bottom surface of the annular convex edge 21 is pressed on the annular groove 41 and connected with the first sealing ring 3, the pressing ring 1 is pressed on the front surface of the annular convex edge 21 and fixedly connected with the liquid injection ring 4, the first sealing ring 3 can be clamped on the annular groove 41, and the pressing ring 1 tightly presses the annular convex edge 21 of the dielectric film 2 and the first sealing ring 3.
Specifically, a second sealing ring 6 can be arranged between the injection ring 4 and the immersion objective lens body 10, an annular groove can be arranged on the inner side of the injection ring 4 or/and the outer side of the immersion objective lens body 10, and the second sealing ring 6 can be clamped in the annular groove, so that the sealing effect is good.
Specifically, clamping ring 1 through retaining member 11 connect in annotate liquid ring 4, retaining member 11 can be the bolt to the dismouting does benefit to follow-up change dielectric film 2, and locks effectually, and is sealed reliable. Of course, the pressing ring 1 may also be connected to the liquid injection ring 4 through a buckle, a thread structure, or the like, and the liquid injection ring 4 may be a metal piece or a hard plastic piece.
Or, the pressing ring 1 may be omitted, the liquid injection ring 4 may be a plastic part, the annular protruding edge 21 of the dielectric film 2 is hermetically connected to the liquid injection ring 4 by hot pressing or ultrasonic welding, and the annular protruding edge 21 of the dielectric film 2 may also be hermetically connected to the liquid injection ring 4.
Specifically, the liquid injection ring 4 is provided with a liquid inlet 42 and a liquid outlet 43, the liquid inlet 42 may be connected to a liquid inlet pipeline through a thread structure or a luer, the liquid outlet 43 may be connected to a liquid outlet pipeline through a thread structure or a luer, and the liquid outlet 43 may discharge gas in the sealed cavity 100. The pressure in the sealed cavity 100 can be adjusted by the pressure adjusting device, so that the dielectric films 2 have different tension degrees, and the focal length can be conveniently adjusted.
Specifically, immersion lens still includes holding ring 5, holding ring 5 fixed cover in outside immersion lens body 10, just holding ring 5 with immersion lens body 10 is coaxial to be set up, the one end of annotating liquid ring 4 is connected holding ring 5, the other end and dielectric film 2, clamping ring 1 of annotating liquid ring 4 are connected. One end of the liquid injection ring 4 can be connected through a thread structure or a screwing structure.
In specific application, the front end of the immersion objective body 10 is provided with a circular truncated cone or frustum-shaped protrusion, and the dielectric film 2 is correspondingly provided with a cylindrical or conical-cylindrical structure.
Specifically, immersion objective still includes loading ring 8, loading ring 8 is located holding ring 5 with between the immersion objective body 10, holding ring 5 threaded connection has setting element 9, setting element 9 support in loading ring 8 to avoid damaging immersion objective body 10. The positioning part 9 can be provided with a plurality of positioning parts which are uniformly distributed along the circumferential direction of the positioning ring 5, so that the positioning part can be conveniently installed and adjusted. The positioning member 9 may be a set screw.
In particular, the immersion objective further comprises a load-relief ring 7, wherein the load-relief ring 7 is located between the load-relief ring 8 and the immersion objective body 10 and plays a role of buffering, so that the immersion objective body 10 can be further prevented from being damaged.
In a specific application, the inner side of the positioning ring 5 may be stepped and includes a first inner side wall and a second inner side wall, and the inner diameter of the positioning ring 5 at the first inner side wall is greater than the inner diameter of the positioning ring at the second inner side wall. The first inside wall department is provided with and is used for annotating the internal thread that liquid ring 4 is connected, annotates the lower terminal surface of liquid ring 4 and can support in the step face between first inside wall and the second inside wall. The loading ring 8 is arranged on the inner side of the second inner side wall, and the unloading ring 7 is arranged on the inner side of the loading ring 8.
In this embodiment, the loading ring 8 is made of a rigid material, such as stainless steel, rigid plastic, etc.; the relief ring 7 may be made of an elastic material, such as rubber, silicone, etc.
During installation, the deloading ring 7 is installed in the suitable position of immersion fluid objective body 10 surface, install the external surface of deloading ring 7 with loading ring 8 again, install the holding ring 5 in the loading ring 8 outside, screw up set screw (setting element 9), and do the fine setting, make holding ring 5 coaxial with immersion fluid objective body 10, will annotate liquid ring 4 again and twist above the holding ring 5, later install first sealing washer 3 on annotating the annular groove 41 on the liquid ring 4, place the dielectric film 2 and annotate liquid ring 4 upper surface, lock the clamping ring 1 through the bolt and annotate on the liquid ring 4, extrude first sealing washer 3, make dielectric film 2 and annotate the reliable sealed of stroke between the liquid ring 4.
During operation, the dielectric film 2, the first sealing ring 3, the liquid injection ring 4, the second sealing ring 6 and the outer surface of the immersion objective lens body 10 form a sealed cavity 100, liquid medium enters the sealed cavity 100 from the liquid injection hole, and air in the sealed cavity 100 is exhausted from the exhaust port 43, so that the sealed cavity 100 is filled with the liquid medium.
Specifically, by testing an Olympus XLUMPLFLN 20XW water immersion objective lens, a liquid medium layer of 2mm can be formed between the medium film 2 and the front surface of the immersion objective lens, and the sample just falls within the working distance of the objective lens, so that the effect is good.
Embodiments of the present invention also provide a microscope having an invertible immersion objective as described above.
The immersion objective lens capable of being used in the inverted mode and the microscope with the immersion objective lens are used in the inverted mode, when a sample is replaced, only the carrying platform for placing the sample needs to be taken out directly, the objective lens does not need to be moved, the space above the sample carrying platform is enough, the size of the sample is not limited by the size of the objective lens and the size of the carrying platform, the height of a main optical path is reduced, the stability of the system is superior to that of the system used in the upright mode, the sample is not directly soaked in a medium, the medium does not need to be replaced frequently, and the use cost of a microscope system is reduced.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. An immersion objective lens capable of being used in an inverted mode is characterized by comprising an immersion objective lens body and a dielectric film arranged at the front end of the immersion objective lens body, wherein the dielectric film has the same refractive index as a liquid medium outside the immersion objective lens body; the immersion objective body is sleeved with a liquid injection ring, a sealing cavity is formed between the dielectric film, the liquid injection ring and the immersion objective body, and a liquid medium is injected into the sealing cavity.
2. The immersion objective lens capable of being used upside down as claimed in claim 1, wherein said liquid injection ring is connected with a pressing ring for pressing said dielectric film against said liquid injection ring, and a first sealing ring is disposed between said dielectric film and said liquid injection ring.
3. The immersion objective lens capable of being used upside down as claimed in claim 2, wherein the periphery of the dielectric film is an annular convex edge, the liquid injection ring is provided with an annular groove for installing the first sealing ring, the bottom surface of the annular convex edge is pressed against the annular groove and connected with the first sealing ring, the pressing ring is pressed against the front surface of the annular convex edge and fixedly connected with the liquid injection ring, and the pressing ring is connected with the liquid injection ring through a locking member.
4. An inverted immersion objective lens as claimed in claim 3, characterized in that the dielectric film is sealingly connected to the filling ring by means of heat pressing or ultrasonic welding.
5. An invertible immersion objective according to claim 1, wherein the injection ring is provided with an inlet and an outlet.
6. An invertible immersion objective according to any of claims 1 to 5, further comprising a positioning ring, which is fitted around the immersion objective body and which is arranged coaxially with the immersion objective body.
7. An immersion objective lens that can be used upside down according to claim 6, characterized in that it further comprises a loading ring, which is located between the positioning ring and the immersion objective lens body, the positioning ring being screwed with a positioning element, which abuts against the loading ring.
8. An invertible immersion objective according to claim 7, wherein the immersion objective further comprises a load-relief ring, which is located between the load ring and the immersion objective body.
9. An invertible immersion objective according to claim 8, wherein the load ring is made of a rigid material; the load relief ring is made of elastic materials.
10. A microscope having an invertible immersion objective as claimed in any of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111151035.1A CN114002839B (en) | 2021-09-29 | 2021-09-29 | Liquid immersion objective capable of being used reversely and microscope with liquid immersion objective |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111151035.1A CN114002839B (en) | 2021-09-29 | 2021-09-29 | Liquid immersion objective capable of being used reversely and microscope with liquid immersion objective |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114002839A true CN114002839A (en) | 2022-02-01 |
| CN114002839B CN114002839B (en) | 2024-06-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111151035.1A Active CN114002839B (en) | 2021-09-29 | 2021-09-29 | Liquid immersion objective capable of being used reversely and microscope with liquid immersion objective |
Country Status (1)
| Country | Link |
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| CN (1) | CN114002839B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001091849A (en) * | 1999-09-21 | 2001-04-06 | Olympus Optical Co Ltd | Liquid immersion objective lens for microscope |
| US20060275918A1 (en) * | 2004-02-16 | 2006-12-07 | Olympus Corporation | Immersion objective lens, retention mechanism for immersion medium, and manufacturing method |
| WO2008099516A1 (en) * | 2007-02-15 | 2008-08-21 | Tokai Hit Co., Ltd. | Objective lens warming unit |
| CN102385078A (en) * | 2011-10-25 | 2012-03-21 | 洛阳师范学院 | Remote-controlled continuous zoom lens with liquid |
| CN203799108U (en) * | 2014-04-24 | 2014-08-27 | 中国人民解放军第三军医大学第一附属医院 | Microscope objective lens bath conversion device |
| US20150212310A1 (en) * | 2014-01-30 | 2015-07-30 | Olympus Corporation | Microscope |
| US20180259761A1 (en) * | 2017-03-07 | 2018-09-13 | Olympus Corporation | Optical system, immersion liquid holder and observation device |
| CN111795734A (en) * | 2019-04-02 | 2020-10-20 | 莱卡微系统Cms有限责任公司 | Device and method for feeding impregnation medium |
| CN112650028A (en) * | 2020-12-25 | 2021-04-13 | 浙江启尔机电技术有限公司 | Immersion liquid supply and recovery device for improving pressure characteristic of immersion flow field |
-
2021
- 2021-09-29 CN CN202111151035.1A patent/CN114002839B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001091849A (en) * | 1999-09-21 | 2001-04-06 | Olympus Optical Co Ltd | Liquid immersion objective lens for microscope |
| US20060275918A1 (en) * | 2004-02-16 | 2006-12-07 | Olympus Corporation | Immersion objective lens, retention mechanism for immersion medium, and manufacturing method |
| WO2008099516A1 (en) * | 2007-02-15 | 2008-08-21 | Tokai Hit Co., Ltd. | Objective lens warming unit |
| CN102385078A (en) * | 2011-10-25 | 2012-03-21 | 洛阳师范学院 | Remote-controlled continuous zoom lens with liquid |
| US20150212310A1 (en) * | 2014-01-30 | 2015-07-30 | Olympus Corporation | Microscope |
| CN203799108U (en) * | 2014-04-24 | 2014-08-27 | 中国人民解放军第三军医大学第一附属医院 | Microscope objective lens bath conversion device |
| US20180259761A1 (en) * | 2017-03-07 | 2018-09-13 | Olympus Corporation | Optical system, immersion liquid holder and observation device |
| CN111795734A (en) * | 2019-04-02 | 2020-10-20 | 莱卡微系统Cms有限责任公司 | Device and method for feeding impregnation medium |
| CN112650028A (en) * | 2020-12-25 | 2021-04-13 | 浙江启尔机电技术有限公司 | Immersion liquid supply and recovery device for improving pressure characteristic of immersion flow field |
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|---|---|
| CN114002839B (en) | 2024-06-25 |
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Effective date of registration: 20240711 Address after: A702, Building 1, Phase 2, Yinxing Zhijie, No. 1301-76, Xinlan Community, Guanlan Street, Longhua District, Shenzhen City, Guangdong Province, 518000 Patentee after: Shenzhen Mingzhun Medical Technology Co.,Ltd. Country or region after: China Address before: Room A101, building 1, Yinxing Zhijie phase II, No. 1301-76, sightseeing Road, Xinlan community, Guanlan street, Longhua District, Shenzhen, Guangdong 518000 Patentee before: Shenzhen National Research Institute of high performance Medical Devices Co.,Ltd. Country or region before: China |
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