CN219831510U - Cell culture and microscopic observation bearing device and cell microscopic observation system - Google Patents
Cell culture and microscopic observation bearing device and cell microscopic observation system Download PDFInfo
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- CN219831510U CN219831510U CN202321334185.0U CN202321334185U CN219831510U CN 219831510 U CN219831510 U CN 219831510U CN 202321334185 U CN202321334185 U CN 202321334185U CN 219831510 U CN219831510 U CN 219831510U
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- microscopic observation
- cell culture
- sealing gasket
- clamping plate
- elastic clamping
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- 238000004113 cell culture Methods 0.000 title claims abstract description 38
- 239000006059 cover glass Substances 0.000 claims abstract description 33
- 238000007789 sealing Methods 0.000 claims abstract description 29
- -1 polytetrafluoroethylene Polymers 0.000 claims abstract description 12
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 12
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000011521 glass Substances 0.000 claims description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 238000000386 microscopy Methods 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 7
- 230000001954 sterilising effect Effects 0.000 abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 230000009194 climbing Effects 0.000 abstract 1
- 238000013461 design Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 238000007334 copolymerization reaction Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture and microscopic observation bearing device and a cell microscopic observation system. The downloader, the sealing gasket and the uploading device can be connected and fixed with the embedded cover glass under the action of the screw; after the cells are accessed, a separate upper cover can be covered on the uploading device. The bearing device has small overall thickness, can be directly fixed on the object carrying bracket of the inverted microscope for use, can be directly used for observing the real-time state of cells under a high-power microscope, and meets the bearing requirement of a slide with the thickness not more than 0.17mm used in a cell climbing sheet. The bearing device can be repeatedly used after sterilization by adopting high-temperature resistant materials such as polytetrafluoroethylene and the like, so that the experiment cost is reduced.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a reusable slide bearing device which can realize high-pressure steam sterilization and cell culture and can directly observe the cell state under an inverted microscope.
Background
The cell culture technology is widely applied to various fields of biology, medicine, new medicine research and development and the like, and is an important experimental science technology. Cell culture and observation are indispensable links in cell culture technology, and for example, some experiments require staining of cell slide and then observation of cell morphology. However, the cell slide fabricated in the experiment is easy to break due to repeated clamping and take out the existing slide, fall off cells and other hidden troubles during elution, incubation and dyeing, and a slide (cover glass) with the thickness not exceeding 0.17mm is usually required to bear the researched object so as to complete the experiment.
At present, a culture device with a slide bearing function mainly adopts a culture container formed by a slide glass and a through hole on a culture plate, and simultaneously the culture plate is connected and fixed with a base, so that cells can be grown on the slide glass at the bottom of the container in a adherence manner, and subsequent operations such as elution, incubation, dyeing and the like are performed in the container, so that the repeated clamping of the slide glass is avoided. However, in order to prevent the device from damaging the slide glass to be carried, the base, the culture plate and other structures are usually designed to be tightly combined with the slide glass by utilizing the reserved assembly gap, and meanwhile, the cross section area of the culture container (namely the effective area of the slide glass) is smaller, so that the whole thickness of the device is larger, and therefore, the observation of the cell state under the inverted microscope is difficult to realize (because the working distance of the objective lens of the inverted microscope, particularly the working distance of the high-power microscope is shorter, for example, the Chinese patent CN201648399U is just used under the upright microscope), and the design thickness of the slide glass is increased (and the use of the commercial slide glass with different specifications is limited).
Disclosure of Invention
The utility model aims to provide a cell culture and microscopic observation bearing device and a cell microscopic observation system, wherein the bearing device has good compatibility with a carrier bracket of an inverted microscope, can improve the consistency of microscopic experiment results, and is convenient for experimental operation.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the cell culture and microscopic observation bearing device comprises a loader, an elastic sealing gasket, an uploading device, a separated upper cover and a fixing screw; the downloader comprises an elastic clamping plate, the uploading device comprises a hard table top plate, a sealing gasket is arranged between the elastic clamping plate and the hard table top plate, a threaded hole and a culture hole (specifically controlling the opening and closing of the upper end of the culture hole) which is opened and closed by a separated upper cover are arranged on the hard table top plate, the lower end of the culture hole is communicated with an upper light passing hole arranged on the sealing gasket, a fixing screw penetrates through the elastic clamping plate and the sealing gasket, and the fixing screw is connected with the threaded hole.
Preferably, the threaded holes on the hard deck plate are symmetrically arranged on two sides of the culture hole, and the elastic clamping plate and the sealing gasket are respectively provided with through holes (for the fixing screws to pass through) corresponding to the positions of the threaded holes.
Preferably, the uploading device (particularly referred to as a hard table panel), the downloading device (particularly referred to as an elastic clamping plate) and the fixing screw are made of polytetrafluoroethylene materials, and the sealing gasket is made of heat-resistant silica gel.
Preferably, the thickness of the uploading device (particularly referred to as a hard table panel) is less than or equal to 10mm, the thickness of the downloading device (particularly referred to as an elastic clamping plate) is less than or equal to 1mm, and the aperture of the culture hole is more than or equal to 20mm.
Preferably, the separated upper cover is made of glass.
Preferably, the bearing device further comprises a cover glass arranged between the sealing gasket and the elastic clamping plate, the elastic clamping plate is provided with a lower light-passing hole, the upper side surface of the cover glass covers the upper light-passing hole on the sealing gasket, and the lower side surface of the cover glass covers the lower light-passing hole on the elastic clamping plate.
Preferably, the cover glass is made of glass and has a thickness of not more than 0.17mm.
A cell microscopic observation system comprises an inverted microscope and the cell culture and microscopic observation bearing device arranged on a carrying bracket of the microscope.
The beneficial effects of the utility model are as follows:
the bearing device of the utility model adopts the upper carrier, the lower carrier and the elastic sealing gasket between the upper carrier and the lower carrier to fix the cover glass to be borne, thus not only ensuring that the formed culture container is well sealed and effectively preventing the liquid in the culture container from leaking out, but also obviously reducing the whole thickness of the bearing device, being capable of being directly fixed on the carrier bracket of the inverted microscope for use and adapting the cover glass with smaller thickness (without limitation to the use of commodity slides with different specifications).
Furthermore, the bearing device of the utility model adopts high temperature resistant materials such as polytetrafluoroethylene, and can be repeatedly sterilized for use, thereby effectively reducing the experiment cost.
Furthermore, when the bearing device is assembled with the cover glass, the downloader, the cover glass, the sealing gasket and the uploading device are only required to be stacked and combined in sequence from bottom to top (the placement of the cover glass can be determined by referring to the positions of the corresponding light transmission holes), and then the assembly is fixed by using screws, so that the operation is simple and convenient.
Drawings
FIG. 1a is a general block diagram (assembled view) of a cell culture and microscopic examination carrier and a cover slip in an embodiment of the present utility model;
FIG. 1b is a schematic view (exploded view) showing the overall structure of a cell culture and microscopic examination carrier and a cover slip according to an embodiment of the present utility model;
FIG. 2a is a top view of the downloader of FIGS. 1a, 1 b;
FIG. 2b is a cross-sectional view of the download device shown in FIGS. 1a and 1 b;
FIG. 3a is a top view of the gasket of FIGS. 1a, 1 b;
FIG. 3b is a cross-sectional view of the gasket of FIGS. 1a and 1 b;
FIG. 4a is a top view of the upload shown in FIGS. 1a, 1 b;
FIG. 4b is a cross-sectional view of the upload shown in FIGS. 1a, 1 b;
FIG. 5 is a schematic view of the set screw of FIGS. 1a and 1 b;
FIG. 6 is a schematic view of the set screw of FIGS. 1a and 1 b;
in the figure: 1-loader, 2-cover glass, 3-sealing gasket, 4-loader, 5-fixing screw, 6-upper cover, 11-lower via hole, 12-lower light passing hole, 31-upper via hole, 32-upper light passing hole, 41-threaded hole and 42-culture hole.
Detailed Description
The utility model is described in further detail below with reference to the drawings and examples. The examples are given solely for the purpose of illustration and are not intended to limit the scope of the utility model.
Design of cell culture and microscopic observation bearing device
Referring to fig. 1a and 1b, the cell culture and microscopic observation carrying device of the present utility model comprises a loader 1, a sealing pad 3, an upper loader 4, a fine tooth fixing screw 5 made of polytetrafluoroethylene, and a separate glass upper cover 6, wherein the device is used for carrying a circular cover glass 2 (commercially available) with a diameter of 26mm and a thickness of 0.17mm, the loader 1, the circular cover glass 2, the sealing pad 3 and the upper loader 4 are combined in the order from bottom to top, and then two fine tooth fixing screws 5 made of polytetrafluoroethylene are screwed into the upper loader 4 through the loader 1 and the sealing pad 3, so that the components are tightly fixed. Wherein:
the downloader 1: the area is 76mm multiplied by 26mm (length 76mm and width 26 mm), the thickness is 1mm, and the polytetrafluoroethylene material is prepared; a large circular hole (as a lower light-passing hole 12) with a radius of 10mm is formed in the middle, two small circular holes (as lower through holes 11 where the fixing screws 5 pass through the downloader 1) with a radius of 5mm are symmetrically formed on two sides of the large circular hole, and the distance from the center of the large circular hole to the center of the small circular hole is 20mm, see fig. 2a and 2b.
Sealing gasket 3: a high-temperature resistant silica gel material with a length of 76mm and a width of 26mm and a thickness of 0.5 mm; a large circular hole (as an upper light-passing hole 32) with a radius of 10mm is formed in the middle, and small circular holes (as upper through holes 31 where the fixing screws 5 penetrate through the sealing gasket 3) with a radius of 5mm are formed on two sides of the large circular hole symmetrically, wherein the distance from the center of the large circular hole to the center of the small circular hole is 20mm, see fig. 3a and 3b.
The uploading device 4: the area is 76mm multiplied by 26mm (length 76mm and width 26 mm), the thickness is 10mm, and the polytetrafluoroethylene material is prepared; a large round hole (as a culture hole 42) with a radius of 10mm is formed in the middle, two small round holes (the inner side walls of the two small round holes are provided with threads, the thread pitch is 1.5mm, namely, the two threaded holes 41 are respectively connected with the fixing screw 5) with a radius of 5mm are symmetrically formed on two sides of the large round hole, and the distance from the center of the large round hole to the center of the small round hole is 20mm, see fig. 4a and 4b.
Fine tooth fixing screw 5 made of polytetrafluoroethylene: the total length is 12mm; the screw cap is a cylinder with the diameter of 12mm and the height of 2mm (a slot is formed in the middle of the cylinder, so that a screw can be conveniently screwed in or dismounted), and the thread pitch is 1.5mm, see fig. 5 and 6.
Separating glass upper cover 6: the area was 76mm by 26mm (length 76mm, width 26 mm) and the thickness was 1mm (commercially available).
In the cell culture and microscopic observation carrying device, a separating type downloader 1 made of polytetrafluoroethylene material with the thickness of 1mm is designed on the lower side of a circular cover glass 2, and the whole device is supported together with a screw cap of a fine tooth fixing screw 5 made of polytetrafluoroethylene material; meanwhile, in order to better protect the fragile circular cover glass 2, a silica gel sheet with the thickness of 0.5mm is used as a sealing gasket 3 between the circular cover glass 2 and the designed separated type upper carrier 4, the circular cover glass 2 is buffered to be in rigid contact with the upper carrier 4, the risk of fragmentation of the circular cover glass 2 is further reduced, the circular cover glass 2 and the upper carrier 4 are also bonded and sealed, and the liquid is effectively prevented from being leaked. The device is mainly made of polytetrafluoroethylene, can be reused after high-pressure sterilization, and has the advantages of cost reduction and environmental protection.
The whole structure of the cell culture and microscopic observation bearing device is designed into a cuboid with the size of 76mm multiplied by 26mm multiplied by 12.67mm, and a circular cover glass 2 (positioned between the upper light passing holes and the lower light passing holes 32 and 12) near the bottom of the device can transmit light rays, so that the cell culture and microscopic observation bearing device can be directly placed on a carrying bracket of an inverted microscope to observe the cell state. The device (specifically, the culture well 42) can form a culture container (column volume: 3.14X10 mm. Times.10mm. Times.10mm=3140 cubic millimeters) with the circular cover glass 2 by a sealing structure design, that is, the culture container can hold about 3mL of culture solution for cell culture.
Therefore, the cell culture and microscopic observation bearing device overcomes the defects of the cell culture devices such as the existing glass slide/cover glass combination, the copolymerization Jiao Min (the glass slide/cover glass combination and the copolymerization Jiao Min are also applicable to high-power mirrors) and the like in cell culture and research: such as easy pollution, low liquid carrying amount, unable to be reused, high experimental cost, etc.
Method for using cell culture and microscopic observation bearing device
(1) Stacking the cleaned downloader 1, the round cover glass 2, the sealing gasket 3 and the uploader 4 together in sequence from bottom to top;
(2) Two fine tooth fixing screws 5 are respectively inserted into small round holes on two sides of the loader 1 and screwed by combining with internal threads of the small round holes on two sides of the loader 4, so that the loader 1, the round cover glass 2 and the sealing gasket 3 are fixed with the loader 4;
(3) Sterilizing the components of the separated glass upper cover 6 and the components fixed together;
(4) After sterilization, the cells are connected into a culture container, and then a separated glass upper cover 6 is covered on an uploading device 4 to prevent bacteria contamination, and then the cells can be cultured according to the conventional steps;
(5) The growth state of the cells can be monitored in real time under an inverted microscope in the culture process; the culture solution can also be discarded, and the microscopic observation experiment can be carried out after the culture solution is directly dyed.
(III) application advantage analysis of cell culture and microscopic observation bearing device
1. Realize compatibility with the object carrying support of the inverted microscope: the structure of the cell culture and microscopic observation bearing device of the utility model refers to the design of a glass slide with the thickness not exceeding 0.17mm, the total height of the device is in the range of the accommodating height of the objective table of the inverted microscope, and the device has better universality than a commercial cylindrical culture dish.
2. Maintaining consistency of observed data: the cell culture and microscopic observation bearing device can be repeatedly sterilized and used, and the effect of the thickness difference of the cover glass on the imaging effect can be basically eliminated by only replacing the cover glass with the same type and batch each time.
3. The experimental cost is greatly reduced: the cell culture and microscopic observation carrying device of the utility model has the cover glass unit price of about 0.15 yuan which needs to be replaced every time, and the use cost of the cell culture and microscopic observation carrying device is about 1.5 percent of that of a disposable cell culture dish with the unit price of about 10 yuan.
In a word, the cell culture and microscopic observation bearing device provided by the utility model is high-temperature resistant and reusable, has good compatibility to common slide glass supports, can effectively ensure the consistency of experimental data, and has higher popularization value.
Claims (8)
1. A cell culture and microscopic observation bearing device, which is characterized in that: the bearing device comprises a downloading device (1), a sealing gasket (3), an uploading device (4), a separated upper cover (6) and a fixing screw (5); the downloader (1) comprises an elastic clamping plate, the uploading device (4) comprises a hard table top plate, the sealing gasket (3) is arranged between the elastic clamping plate and the hard table top plate, a threaded hole (41) and a culture hole (42) which is controlled to be opened and closed by a separated upper cover (6) are arranged on the hard table top plate, the culture hole (42) is communicated with a light passing hole arranged on the sealing gasket (3), and the fixing screw (5) penetrates through the elastic clamping plate and the sealing gasket (3) and is connected with the threaded hole (41).
2. The cell culture and microscopic observation carrying device according to claim 1, wherein: the threaded holes (41) are symmetrically arranged on two sides of the culture hole (42), and through holes opposite to the threaded holes (41) are respectively formed in the elastic clamping plate and the sealing gasket (3).
3. The cell culture and microscopic observation carrying device according to claim 1, wherein: the hard deck plate, the elastic clamping plate and the fixing screws (5) are made of polytetrafluoroethylene materials, and the sealing gasket (3) is made of heat-resistant silica gel.
4. The cell culture and microscopic observation carrying device according to claim 1, wherein: the thickness of the hard deck plate is less than or equal to 10mm, the thickness of the elastic clamping plate is less than or equal to 1mm, and the aperture of the culture hole (42) is more than or equal to 20mm.
5. The cell culture and microscopic observation carrying device according to claim 1, wherein: the separated upper cover (6) is made of glass.
6. The cell culture and microscopic observation carrying device according to claim 1, wherein: the bearing device further comprises a cover glass (2) arranged between the sealing gasket (3) and the elastic clamping plate, the elastic clamping plate is provided with a light-passing hole, and two side surfaces of the cover glass (2) are respectively covered with the sealing gasket (3) and the light-passing hole on the elastic clamping plate.
7. The cell culture and microscopic observation carrying device according to claim 6, wherein: the cover glass (2) is made of glass, and the thickness of the cover glass is not more than 0.17mm.
8. A cell microscopic observation system, characterized in that: a cell culture and microscopy carrier according to any one of claims 1 to 7 comprising an inverted microscope and a carrier for the microscope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321334185.0U CN219831510U (en) | 2023-05-29 | 2023-05-29 | Cell culture and microscopic observation bearing device and cell microscopic observation system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321334185.0U CN219831510U (en) | 2023-05-29 | 2023-05-29 | Cell culture and microscopic observation bearing device and cell microscopic observation system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219831510U true CN219831510U (en) | 2023-10-13 |
Family
ID=88274858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321334185.0U Active CN219831510U (en) | 2023-05-29 | 2023-05-29 | Cell culture and microscopic observation bearing device and cell microscopic observation system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219831510U (en) |
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2023
- 2023-05-29 CN CN202321334185.0U patent/CN219831510U/en active Active
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