CN112210499A - Cell negative pressure culture apparatus - Google Patents
Cell negative pressure culture apparatus Download PDFInfo
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- CN112210499A CN112210499A CN202011218223.7A CN202011218223A CN112210499A CN 112210499 A CN112210499 A CN 112210499A CN 202011218223 A CN202011218223 A CN 202011218223A CN 112210499 A CN112210499 A CN 112210499A
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- culture
- cell culture
- negative pressure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/14—Pressurized fluid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The invention provides a negative pressure cell culture device, and relates to the technical field of medical instruments. The cell negative pressure culture device comprises: the device comprises a cell culture perforated plate, a cylindrical culture substrate, a cell culture vessel, a silica gel sealing cover, a first needle and a second needle; the cell culture perforated plate is provided with a plurality of culture tanks; the bottom surface of the cell culture vessel is made of a porous membrane; the cell culture vessel is internally provided with foam. According to the invention, a culture substrate is in sealed insertion connection with a culture tank, a cell culture vessel is in sealed insertion connection with the culture substrate, and a silica gel sealing cover seals an opening at the top of the cell culture vessel, so that a sterile sealed environment is provided for cell culture; the negative pressure pump provides negative pressure for the culture environment of the cells through the first needle head; the culture solution is injected into the bottom of the culture tank through the second needle, so that the culture solution is injected in real time under the sterile condition of keeping negative pressure sealing; effectively avoids the cell contamination and simultaneously ensures the accuracy of cell culture observation.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a negative pressure cell culture device.
Background
In medical research, it is sometimes necessary to observe the growth of cells in a negative pressure environment.
At present, in order to observe the growth of cells under a negative pressure environment, cells are generally cultured on a cell culture multi-well plate and a cell culture vessel, and a quantitative culture solution is injected at one time before negative pressure sealing.
In the culture process, if the culture solution is required to be injected continuously, the negative pressure sealing state of the cell culture device must be released. In the process of injecting the culture solution, the sterile sealing environment and the negative pressure state of the cell culture device are destroyed, cells are easily polluted, and the observation accuracy is influenced.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a negative pressure cell culture device, which solves the problem that a negative pressure cell culture device cannot inject culture solution in real time under the aseptic condition of maintaining negative pressure sealing.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
a negative cell pressure culture device, comprising: the device comprises a cell culture perforated plate, a cylindrical culture substrate, a cell culture vessel, a silica gel sealing cover, a first needle and a second needle;
the cell culture perforated plate is provided with a plurality of culture tanks; the bottom surface of the cell culture vessel is made of a polycarbonate porous membrane subjected to culture treatment; foam is arranged in the cell culture vessel;
the culture substrate is inserted into the culture groove, and the cell culture vessel is inserted into the culture substrate; the outer cylinder wall of the culture substrate is hermetically connected with the inner side wall of the culture tank, and the outer side wall of the cell culture vessel is hermetically connected with the inner cylinder wall of the culture substrate; the silica gel sealing cover seals the top opening of the cell culture vessel;
the first needle penetrates through the silica gel sealing cover to be inserted into the foam, and the second needle penetrates through the silica gel sealing cover and the culture substrate in sequence to be inserted into the bottom of the culture tank; the tail end of the first needle head is communicated with a negative pressure pump, and the tail end of the second needle head is communicated with a culture solution injection device.
Preferably, the top surface of the foam is covered with a sealing film.
Preferably, a fixing pad is arranged on the sealing membrane, and the first needle is inserted into the foam through the fixing pad.
Preferably, the outer cylinder wall of the culture substrate is provided with an annular first sealing groove, and the inner cylinder wall is provided with an annular second sealing groove; a first sealing ring is arranged in the first sealing groove, and a second sealing ring is arranged in the second sealing groove; the outer cylinder wall of the culture substrate and the inner side wall of the culture tank are sealed through a first sealing ring, and the inner cylinder wall of the culture substrate and the outer side wall of the cell culture vessel are sealed through a second sealing ring.
Preferably, the culture substrate is provided with a through hole for the second needle to pass through, and the top end of the through hole is provided with a chamfer angle.
Preferably, the bottom surface of the culture substrate is provided with a plurality of through grooves extending upwards, the through grooves are communicated with the space in the culture substrate barrel, and the bottom ends of the through holes are communicated with the through grooves.
Preferably, the top surface of the culture substrate is provided with a downwardly extending limiting groove, the outer side wall of the cell culture vessel is provided with limiting clamping blocks in one-to-one correspondence with the limiting groove, and the limiting clamping blocks are inserted into the limiting groove.
Preferably, the first sealing ring has a larger diameter in cross section than the second needle, and the second needle is inserted into and passes through the first sealing ring during the process of passing through the culture substrate.
(III) advantageous effects
The invention provides a negative pressure cell culture device. Compared with the prior art, the method has the following beneficial effects:
according to the invention, cell tissues to be cultured are placed between the inner bottom surface of a cell culture vessel and foam cotton, a culture substrate is in sealed insertion connection with a culture tank, the cell culture vessel is in sealed insertion connection with the culture substrate, and a silica gel sealing cover seals an opening at the top of the cell culture vessel, so that a sterile sealed environment is provided for cell culture; in the process of cell culture, a negative pressure pump provides negative pressure for the culture environment of cells through a first needle head; the culture solution is injected into the bottom of the culture tank through the second needle head and then enters the cell culture vessel through the bottom surface of the porous membrane of the cell culture vessel, so that the culture solution is injected in real time under the sterile condition of keeping negative pressure sealing; effectively avoids the cell contamination and simultaneously ensures the accuracy of cell culture observation.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is an exploded view of a negative pressure cell culture apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic view of the internal structure of a negative pressure cell culture apparatus according to an embodiment of the present invention;
FIG. 3 is an isometric view of a culture substrate in an example of the invention;
FIG. 4 is a bottom view of a culture substrate according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the application provides a cell negative pressure culture device, and solves the problem that the cell negative pressure culture device cannot inject culture solution in real time under the sterile condition of keeping negative pressure sealing.
In order to solve the technical problems, the general idea of the embodiment of the application is as follows:
in the embodiment of the invention, the cell tissue to be cultured is arranged between the inner bottom surface of the cell culture vessel and the foam, the culture substrate is in sealed insertion connection with the culture tank, the cell culture vessel is in sealed insertion connection with the culture substrate, and the top opening of the cell culture vessel is sealed by the silica gel sealing cover, so that a sterile sealing environment is provided for cell culture; in the process of cell culture, a negative pressure pump provides negative pressure for the culture environment of cells through a first needle head; the culture solution is injected into the bottom of the culture tank through the second needle head and then enters the cell culture vessel through the bottom surface of the porous membrane of the cell culture vessel, so that the culture solution is injected in real time under the sterile condition of keeping negative pressure sealing; effectively avoids the cell contamination and simultaneously ensures the accuracy of cell culture observation.
In order to better understand the technical solution, the technical solution will be described in detail with reference to the drawings and the specific embodiments.
Example (b):
as shown in fig. 1 and 2, the present invention provides a cell negative pressure culture apparatus including: a cell culture perforated plate 10, a cylindrical culture substrate 20, a cell culture vessel 30, a silica gel sealing cover 40, a first needle 41 and a second needle 42;
the cell culture perforated plate 10 is provided with a plurality of culture tanks 11; the bottom surface of the cell culture vessel 30 is made of a polycarbonate porous membrane subjected to culture treatment; the cell culture vessel 30 is internally provided with foam 31;
the culture substrate 20 is inserted into the culture tank 11, and the cell culture vessel 30 is inserted into the culture substrate 20; the outer cylinder wall of the culture substrate 20 is hermetically connected with the inner side wall of the culture tank 11, and the outer side wall of the cell culture vessel 30 is hermetically connected with the inner cylinder wall of the culture substrate 20; the silica gel sealing cover 40 seals the top opening of the cell culture vessel 30;
the first needle 41 penetrates through the silica gel sealing cover 40 and is inserted into the foam 31, and the second needle 42 penetrates through the silica gel sealing cover 40 and the culture substrate 20 in sequence and is inserted into the bottom of the culture tank 11; the end of the first needle 41 is communicated with a negative pressure pump (not shown in the figure), and the end of the second needle 42 is communicated with a culture solution injection device (not shown in the figure).
The cell tissue to be cultured is placed between the inner bottom surface of the cell culture vessel 30 and the foam 31, the culture substrate 20 is in sealed insertion connection with the culture tank 11, the cell culture vessel 30 is in sealed insertion connection with the culture substrate 20, and the silica gel sealing cover 40 seals the top opening of the cell culture vessel 30, so that a sterile sealing environment is provided for cell culture; in the process of cell culture, the negative pressure pump provides negative pressure for the culture environment of cells through the first needle 41; the culture solution is injected into the bottom of the culture tank 11 through the second needle 42 and then enters the cell culture vessel 30 through the bottom surface of the porous membrane of the cell culture vessel 30, so that the culture solution is injected in real time under the sterile condition of maintaining negative pressure sealing; effectively avoids the cell contamination and simultaneously ensures the accuracy of cell culture observation.
As shown in figure 2, the top surface of the foam 31 is covered with a sealing film 32, so that a structure similar to a dressing is formed, and the sealing film 32 has the function of blocking external pollutants and is beneficial to keeping the foam 31 moist and promoting the formation of cell tissues.
As shown in fig. 1 and 2, a fixing pad 33 is disposed on the sealing membrane 32, the first needle 41 is inserted into the foam 31 through the fixing pad 33, the fixing pad 33 is used for fixing the needle point of the first needle 41 and sealing the first needle 41 which passes through, so that the negative pressure pump can provide an effective negative pressure environment for cell culture through the first needle 41.
As shown in fig. 1 to 3, an annular first sealing groove 21 is formed in the outer cylindrical wall of the culture substrate 20, and an annular second sealing groove 22 is formed in the inner cylindrical wall; a first sealing ring 23 is arranged in the first sealing groove 21, and a second sealing ring 24 is arranged in the second sealing groove 22; the outer wall of the culture substrate 20 and the inner wall of the culture tank 11 are sealed by a first seal ring 23, and the inner wall of the culture substrate 20 and the outer wall of the cell culture vessel 30 are sealed by a second seal ring 24.
As shown in fig. 3, the culture substrate 20 is provided with a through hole 25 for the second needle 42 to pass through, and the top end of the through hole 25 is provided with a chamfer to facilitate the insertion of the second needle 42.
As shown in fig. 3 and 4, the bottom surface of the culture substrate 20 is provided with a plurality of through grooves 26 extending upward, the through grooves 26 are communicated with the space in the cylinder of the culture substrate 20, and the bottom ends of the through holes 25 are communicated with the through grooves 26, so that the culture solution can be conveniently injected into the culture tank 11 through the second needles 42.
As shown in fig. 1, the top surface of the culture substrate 20 is provided with a downwardly extending limiting groove 27, the outer side wall of the cell culture vessel 30 is provided with limiting blocks 34 corresponding to the limiting groove 27 one by one, and the limiting blocks 34 are inserted into the limiting groove 27 to prevent the cell culture vessel 30 from rotating relative to the culture substrate 20.
As shown in FIG. 1, the diameter of the cross section of the first sealing ring 23 is larger than that of the second needle 42, and the second needle 42 is inserted into and passes through the first sealing ring 23 during the process of passing through the culture substrate 20, thereby not only damaging the seal of the first sealing ring 23 to the culture substrate 20 and the culture tank 11, but also sealing the second needle 42.
In summary, compared with the prior art, the invention has the following beneficial effects:
in the embodiment of the invention, the cell tissue to be cultured is arranged between the inner bottom surface of the cell culture vessel and the foam, the culture substrate is in sealed insertion connection with the culture tank, the cell culture vessel is in sealed insertion connection with the culture substrate, and the top opening of the cell culture vessel is sealed by the silica gel sealing cover, so that a sterile sealing environment is provided for cell culture; in the process of cell culture, a negative pressure pump provides negative pressure for the culture environment of cells through a first needle head; the culture solution is injected into the bottom of the culture tank through the second needle head and then enters the cell culture vessel through the bottom surface of the porous membrane of the cell culture vessel, so that the culture solution is injected in real time under the sterile condition of keeping negative pressure sealing; effectively avoids the cell contamination and simultaneously ensures the accuracy of cell culture observation.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. A negative pressure cell culture device, comprising: a cell culture perforated plate (10), a cylindrical culture substrate (20), a cell culture vessel (30), a silica gel sealing cover (40), a first needle head (41) and a second needle head (42);
the cell culture perforated plate (10) is provided with a plurality of culture tanks (11); the bottom surface of the cell culture vessel (30) is made of a polycarbonate porous membrane which is subjected to culture treatment; foam (31) is arranged in the cell culture vessel (30);
the culture substrate (20) is inserted into the culture tank (11), and the cell culture vessel (30) is inserted into the culture substrate (20); the outer cylinder wall of the culture substrate (20) is hermetically connected with the inner side wall of the culture tank (11), and the outer side wall of the cell culture vessel (30) is hermetically connected with the inner cylinder wall of the culture substrate (20); the silica gel sealing cover (40) is used for sealing the top opening of the cell culture vessel (30);
the first needle (41) penetrates through the silica gel sealing cover (40) and is inserted into the foam (31), and the second needle (42) penetrates through the silica gel sealing cover (40) and the culture substrate (20) in sequence and is inserted into the bottom of the culture tank (11); the tail end of the first needle (41) is communicated with a negative pressure pump, and the tail end of the second needle (42) is communicated with a culture solution injection device.
2. The negative pressure cell culture device according to claim 1, wherein the top surface of the foam (31) is covered with a sealing film (32).
3. The negative pressure cell culture device according to claim 2, wherein the sealing membrane (32) is provided with a fixing pad (33), and the first needle (41) is inserted into the foam (31) through the fixing pad (33).
4. The negative pressure cell culture device according to claim 1, wherein the outer cylinder wall of the culture substrate (20) is provided with an annular first sealing groove (21), and the inner cylinder wall is provided with an annular second sealing groove (22); a first sealing ring (23) is arranged in the first sealing groove (21), and a second sealing ring (24) is arranged in the second sealing groove (22); the outer cylinder wall of the culture substrate (20) and the inner side wall of the culture tank (11) are sealed through a first sealing ring (23), and the inner cylinder wall of the culture substrate (20) and the outer side wall of the cell culture vessel (30) are sealed through a second sealing ring (24).
5. The negative pressure cell culture device according to claim 1, wherein the culture substrate (20) is provided with a through hole (25) for the second needle (42) to pass through, and the top end of the through hole (25) is chamfered.
6. The negative pressure cell culture device according to claim 5, wherein the bottom surface of the culture substrate (20) is provided with a plurality of through grooves (26) extending upwards, the through grooves (26) are communicated with the space in the cylinder of the culture substrate (20), and the bottom ends of the through holes (25) are communicated with the through grooves (26).
7. The negative pressure cell culture device according to claim 1, wherein the top surface of the culture substrate (20) is provided with a downwardly extending limiting groove (27), the outer side wall of the cell culture vessel (30) is provided with limiting blocks (34) corresponding to the limiting groove (27), and the limiting blocks (34) are inserted into the limiting groove (27).
8. The negative pressure cell culture device according to claim 4, wherein the first sealing ring (23) has a larger diameter in cross section than the second needle (42), and the second needle (42) is inserted into and passes through the first sealing ring (23) during the process of passing through the culture substrate (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011218223.7A CN112210499A (en) | 2020-11-04 | 2020-11-04 | Cell negative pressure culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011218223.7A CN112210499A (en) | 2020-11-04 | 2020-11-04 | Cell negative pressure culture apparatus |
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| Publication Number | Publication Date |
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| CN112210499A true CN112210499A (en) | 2021-01-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202011218223.7A Pending CN112210499A (en) | 2020-11-04 | 2020-11-04 | Cell negative pressure culture apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7286749B1 (en) | 2021-12-23 | 2023-06-05 | 財團法人工業技術研究院 | Cell and tissue sheet forming package and cell injection device |
-
2020
- 2020-11-04 CN CN202011218223.7A patent/CN112210499A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7286749B1 (en) | 2021-12-23 | 2023-06-05 | 財團法人工業技術研究院 | Cell and tissue sheet forming package and cell injection device |
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