CN214167996U

CN214167996U - Culture apparatus for reinforcing mesenchymal stem cell treatment osteoarthritis

Info

Publication number: CN214167996U
Application number: CN202022890451.0U
Authority: CN
Inventors: 田程; 赵敬军; 张大军
Original assignee: Zhengzhou Aobo Cell Medicine Laboratory Co ltd
Current assignee: Zhengzhou Aobo Cell Medicine Laboratory Co ltd
Priority date: 2020-12-04
Filing date: 2020-12-04
Publication date: 2021-09-10
Anticipated expiration: 2030-12-04

Abstract

The utility model provides a reinforcing mesenchymal stem cell treats culture apparatus of osteoarthritis. This reinforcing mesenchymal stem cell treats culture apparatus of osteoarthritis, includes: an incubator; the first motor is fixedly arranged on the inner wall of the bottom of the incubator; the first rotating rod is fixedly arranged on an output shaft of the first motor; the rotary table is fixedly arranged at the top end of the first rotary rod; the four culture dishes are all arranged on the turntable; two litter, two litter are all fixed mounting in the incubator. The utility model provides a culture apparatus of reinforcing mesenchymal stem cell treatment osteoarthritis has the advantage of quantitative transport culture solution, a plurality of culture dishes of ability simultaneous culture.

Description

Culture apparatus for reinforcing mesenchymal stem cell treatment osteoarthritis

Technical Field

The utility model belongs to the cell culture field especially relates to a culture apparatus of reinforcing mesenchymal stem cell treatment osteoarthritis.

Background

Bone marrow mesenchymal stem cells, which are known as bone marrow stromal fibroblasts in the past, are a class of adult stem cells derived from mesoderm, have the potential of self-renewal and multidirectional differentiation, and can be differentiated into various mesenchymal tissues, such as bones, cartilages, fats, bone marrow hematopoietic tissues and the like. Migration, colonization, proliferation and differentiation of bone marrow BMSCs requires information to be obtained from the outside. The direction of differentiation depends on the microenvironment in which it is located. The "microenvironment" herein includes not only the bone marrow microenvironment, but also the local interstitial tissue microenvironment. In the related art, a culture device for enhancing mesenchymal stem cells to treat osteoarthritis is disclosed, which comprises a culture box and a culture solution providing device; the two opposite inner walls of the incubator are respectively provided with a first clamping device and a second clamping device from top to bottom in sequence, a first ELISA plate is clamped between the two first clamping devices, and a second ELISA plate is clamped between the two second clamping devices. The beneficial effects are as follows: the aim of culturing the mesenchymal stem cells can be fulfilled by the arranged culture box; the purpose of clamping the ELISA plate for culturing the mesenchymal stem cells can be achieved through the arranged first clamping device and the second clamping device; the purpose of providing the culture solution can be achieved through the arranged culture solution providing device.

However, there are disadvantages in the above structure, because the transfer amount of the culture solution needs to be accurately controlled for the culture of the mesenchymal stem cells, and the culture solution is sprayed through the first branch pipe or the second branch pipe in the above device, the amount of the culture solution entering the corresponding hole of the microplate can not be accurately controlled, which may cause the culture failure of the mesenchymal stem cells.

Therefore, there is a need to provide a new culture device for enhancing mesenchymal stem cells to treat osteoarthritis to solve the above technical problems.

SUMMERY OF THE UTILITY MODEL

The utility model provides a technical problem provide a culture solution is carried to ration, can cultivate the culture apparatus of reinforcing mesenchymal stem cell treatment osteoarthritis of a plurality of culture dishes simultaneously.

In order to solve the technical problem, the utility model provides a reinforcing mesenchymal stem cell treats culture apparatus of osteoarthritis, include: an incubator; the first motor is fixedly arranged on the inner wall of the bottom of the incubator; the first rotating rod is fixedly arranged on an output shaft of the first motor; the rotary table is fixedly arranged at the top end of the first rotary rod; the four culture dishes are all arranged on the turntable; the two sliding rods are fixedly arranged in the incubator; the springs are sleeved on the corresponding sliding rods in a sliding manner, and the top ends of the springs are fixedly connected with the inner wall of the top of the incubator; the sliding blocks are slidably mounted on the two sliding rods, and the tops of the sliding blocks are fixedly connected with the bottom ends of the springs; the second motor is fixedly arranged on the inner wall of one side of the incubator; the residual wheel is fixedly arranged on an output shaft of the second motor; the connecting block is hinged to the outer wall of one side of the sliding block; the nutrient box is arranged in the incubator, and the outer wall of one side of the nutrient box is hinged with one side of the connecting block; the second rotating rod is rotatably arranged on the nutrient box, and one end of the second rotating rod is fixedly connected with the inner wall of one side of the nutrient box; the liquid level sensor is fixedly arranged on the inner wall of one side of the nutrient box; the controller is fixedly arranged at the top of the incubator.

As a further proposal of the utility model, the top fixed mounting of the incubator has a feed pump, four universal wheels are installed to the bottom rectangle of the incubator, and the inlet port has been seted up to one side of the incubator.

As a further proposal of the utility model, the inlet opening fixed mounting of the feeding pump has the inlet pipe, the apopore fixedly connected with discharging pipe of the feeding pump, and the one end of the discharging pipe extends to the incubator.

As a further proposal of the utility model, two slide holes have been seted up on the slider, and equal activity is inlayed in two slide holes has the ball, and two litter run through corresponding slide hole and with ball sliding connection.

As the utility model discloses a further scheme has seted up four recesses on the carousel, and equal fixed mounting has a plurality of rubber protection pieces in four recesses, and four culture dishes are respectively for being located four recesses, and one side outer wall fixed mounting of slider has the rack, and the rack meshes with the incomplete wheel mutually.

As a further proposal of the utility model, the second bull stick is sleeved with a bearing, a round hole is arranged on the outer wall of one side of the nutrient box, and the outer ring of the bearing is fixedly connected with the inner wall of the round hole.

Compared with the prior art, the utility model provides a culture apparatus of reinforcing mesenchymal stem cell treatment osteoarthritis has following beneficial effect:

the utility model has the advantages that the culture solution is poured into the culture dish quantitatively under the mutual cooperation of the feeding pump, the discharging pipe, the second motor, the residual wheel, the rack, the slide block, the spring, the connecting block, the nutrient box and the second rotating rod, and the liquid level sensor and the controller, and the culture solution is conveyed quantitatively; through mutually supporting of first bull stick, carousel, culture dish under, carry the culture solution to four culture dishes, have the advantage that can cultivate a plurality of culture dishes simultaneously.

Drawings

In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.

Fig. 1 is a schematic front sectional view of a preferred embodiment of a culture device for enhancing mesenchymal stem cells to treat osteoarthritis according to the present invention;

FIG. 2 is an enlarged schematic view of portion A of FIG. 1;

fig. 3 is an enlarged schematic view of a portion B in fig. 1.

In the figure: 1. an incubator; 2. a first motor; 3. a first rotating lever; 4. a turntable; 5. a culture dish; 6. a slide rod; 7. a spring; 8. a slider; 9. a second motor; 10. a residual wheel; 11. connecting blocks; 12. a nutrient box; 13. a second rotating rod; 14. a liquid level sensor; 15. a controller; 16. a feeding pump.

Detailed Description

Referring to fig. 1, 2 and 3 in combination, a culture device for enhancing mesenchymal stem cells to treat osteoarthritis includes: an incubator 1; the first motor 2 is fixedly arranged on the inner wall of the bottom of the incubator 1; the top of the incubator 1 is fixedly provided with a feed pump 16, the bottom of the incubator 1 is rectangularly provided with four universal wheels, one side of the incubator 1 is provided with an air inlet first rotating rod 3, and the first rotating rod 3 is fixedly arranged on an output shaft of a first motor 2; the rotary table 4 is fixedly arranged at the top end of the first rotary rod 3; four culture dishes 5, wherein the four culture dishes 5 are all arranged on the turntable 4; a feed pipe is fixedly arranged at a water inlet of the feed pump 16, a discharge pipe is fixedly connected with a water outlet of the feed pump 16, and one end of the discharge pipe extends into the incubator 1; the two sliding rods 6, the two sliding rods 6 are both fixedly arranged in the incubator 1; the springs 7 are sleeved on the corresponding sliding rods 6 in a sliding manner, and the top ends of the springs 7 are fixedly connected with the inner wall of the top of the incubator 1; the sliding blocks 8 are arranged on the two sliding rods 6 in a sliding mode, and the top of each sliding block 8 is fixedly connected with the bottom end of the spring 7; the second motor 9, the second motor 9 is fixedly installed on one side of the inner wall of the incubator 1; two slide holes are formed in the slide block 8, balls are movably embedded in the two slide holes, and the two slide rods 6 penetrate through the corresponding slide holes and are connected with the balls in a sliding manner; the residual wheel 10 is fixedly arranged on the output shaft of the second motor 9; the connecting block 11 is hinged to the outer wall of one side of the sliding block 8; the nutrient box 12 is arranged in the incubator 1, and the outer wall of one side of the nutrient box 12 is hinged with one side of the connecting block 11; four grooves are formed in the turntable 4, a plurality of rubber protection blocks are fixedly mounted in the four grooves, the four culture dishes 5 are respectively positioned in the four grooves, a rack is fixedly mounted on the outer wall of one side of the sliding block 8, and the rack is meshed with the residual wheel 10; the second rotating rod 13 is rotatably arranged on the nutrient box 12, and one end of the second rotating rod 13 is fixedly connected with the inner wall of one side of the nutrient box 12; the liquid level sensor 14, the liquid level sensor 14 is fixedly installed on one side inner wall of the nutrient box 12; a bearing is sleeved on the second rotating rod 12, a round hole is formed in the outer wall of one side of the nutrient box 12, and the outer ring of the bearing is fixedly connected with the inner wall of the round hole; and the controller 15, wherein the controller 15 is fixedly arranged on the top of the incubator 1.

The utility model provides a culture apparatus for reinforcing mesenchymal stem cell treatment osteoarthritis's theory of operation as follows:

the device can move through the four universal wheels and enter air through the air inlet;

when culture solution needs to be quantitatively conveyed to the culture dish 5, the feeding pump 16 is started, and when the nutrient solution in the nutrient box 12 touches the liquid level sensor 14, the liquid level sensor 14 sends a signal to enable the controller 17 to close the feeding pump 16, so that the nutrient solution can be quantitatively conveyed;

starting a second motor 9 to drive a residual wheel 10 to rotate, driving a rack and a slide block 8 to move upwards by the residual wheel 10, driving a connecting block 11 to rotate towards the upper right by the slide block 8, driving a nutrient box 12 to rotate towards the right by the connecting block 11, and pouring nutrient solution into the culture dish 5;

when the cell in the culture dish 5 of difference needs is cultivateed, start first motor 2 and drive first bull stick 3 and rotate, first bull stick 3 drives carousel 4 and culture dish 5 and rotates, when another culture dish 5 rotates the below of nourishment box 12, stops first motor 2, restarts feeding pump 16 and carries out the ration nutrient solution.

The utility model discloses a feed pump 16, the discharging pipe, second motor 9, incomplete wheel 10, the rack, slider 8, spring 7, connecting block 11, nourishment box 12, second bull stick 13, under the mutually supporting of liquid level inductor 14 and controller 15, the culture solution is poured into to culture dish 5 to the ration, has the advantage of quantitative transport culture solution, under mutually supporting through first bull stick 3, carousel 4, culture dish 5, carry the culture solution to four culture dishes 5, have the advantage that can cultivate a plurality of culture dishes simultaneously.

It should be noted that the device structure and the accompanying drawings of the present invention mainly describe the principle of the present invention, and in the design principle, the settings of the power mechanism, the power supply system, the control system, etc. of the device are not completely described, and on the premise that the skilled person understands the principle of the present invention, the details of the power mechanism, the power supply system, and the control system can be clearly known, the control mode of the application file is automatically controlled by the controller, and the control circuit of the controller can be realized by simple programming of the skilled person in the art;

the standard parts used in the method can be purchased from the market, and can be customized according to the description of the specification and the accompanying drawings, the specific connection mode of each part adopts conventional means such as mature bolts, rivets, welding and the like in the prior art, the machines, parts and equipment adopt conventional models in the prior art, and the structure and the principle of the parts known by the skilled person can be known by technical manuals or conventional experimental methods.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments, or a direct or indirect use of these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the claims and their equivalents, which are to be included within the scope of the invention as defined in the claims.

Claims

1. A culture device for enhancing mesenchymal stem cells to treat osteoarthritis, comprising:

an incubator;

the first motor is fixedly arranged on the inner wall of the bottom of the incubator;

the first rotating rod is fixedly arranged on an output shaft of the first motor;

the rotary table is fixedly arranged at the top end of the first rotary rod;

the four culture dishes are all arranged on the rotary plate;

the two sliding rods are fixedly arranged in the incubator;

the springs are sleeved on the corresponding sliding rods in a sliding mode, and the top ends of the springs are fixedly connected with the inner wall of the top of the incubator;

the sliding blocks are slidably mounted on the two sliding rods, and the tops of the sliding blocks are fixedly connected with the bottom ends of the springs;

the second motor is fixedly arranged on the inner wall of one side of the incubator;

the residual wheel is fixedly arranged on an output shaft of the second motor;

the connecting block is hinged to the outer wall of one side of the sliding block;

the nutrient box is arranged in the incubator, and the outer wall of one side of the nutrient box is hinged with one side of the connecting block;

the second rotating rod is rotatably arranged on the nutrient box, and one end of the second rotating rod is fixedly connected with the inner wall of one side of the nutrient box;

the liquid level sensor is fixedly arranged on the inner wall of one side of the nutrient box;

and the controller is fixedly arranged at the top of the incubator.

2. The culture device for enhancing mesenchymal stem cells to treat osteoarthritis according to claim 1, wherein a feed pump is fixedly mounted at the top of the culture box, four universal wheels are mounted at the bottom of the culture box in a rectangular shape, and an air inlet hole is formed in one side of the culture box.

3. The culture device for enhancing mesenchymal stem cells to treat osteoarthritis according to claim 2, wherein a feed pipe is fixedly installed at a water inlet of the feed pump, a discharge pipe is fixedly connected to a water outlet of the feed pump, and one end of the discharge pipe extends into the culture box.

4. The culture device for enhancing mesenchymal stem cells to treat osteoarthritis according to claim 1, wherein the slider is provided with two slide holes, balls are movably embedded in the two slide holes, and the two slide rods penetrate through the corresponding slide holes and are slidably connected with the balls.

5. The culture device for enhancing mesenchymal stem cells to treat osteoarthritis according to claim 1, wherein four grooves are formed in the turntable, a plurality of rubber protection blocks are fixedly mounted in each of the four grooves, the four culture dishes are respectively positioned in the four grooves, a rack is fixedly mounted on an outer wall of one side of the sliding block, and the rack is meshed with the residual wheel.

6. The culture device for enhancing mesenchymal stem cells to treat osteoarthritis according to claim 1, wherein a bearing is sleeved on the second rotating rod, a circular hole is formed in the outer wall of one side of the nutrient box, and the outer ring of the bearing is fixedly connected with the inner wall of the circular hole.