CN113862115B - Placenta mesenchymal stem cell multiple extraction element - Google Patents
Placenta mesenchymal stem cell multiple extraction element Download PDFInfo
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- CN113862115B CN113862115B CN202111242719.2A CN202111242719A CN113862115B CN 113862115 B CN113862115 B CN 113862115B CN 202111242719 A CN202111242719 A CN 202111242719A CN 113862115 B CN113862115 B CN 113862115B
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Abstract
The invention discloses a multiple extraction device for placenta mesenchymal stem cells, which belongs to the technical field of cell extraction and comprises a test box body, a motor I, cell extraction equipment and diluent adding equipment.
Description
Technical Field
The invention relates to the technical field of cell extraction, in particular to a multiple extraction device for placenta mesenchymal stem cells.
Background
The placenta mesenchymal stem cell is a pluripotent stem cell and is a pluripotent cell with self-replication capacity. Under certain conditions, it can differentiate into a variety of functional cells like APSC pluripotent cells. Derived from mesoderm during embryonic development. MSCs are an important cell bank involved in tissue regeneration during normal tissue injury repair in the body. Under the action of special signals caused by tissue injury, MSC migrate to the injured part, locally accumulate and proliferate, and differentiate along different paths according to different injury signals. MSC is easy to separate and amplify, has vigorous in vitro multiplication capacity, and can maintain the multi-directional differentiation capacity even if amplified by 1 hundred million times. Therefore, MSC is a practical seed cell for tissue repair.
Through retrieval, chinese patent number CN202020222685.5 discloses a placenta mesenchymal stem cell multi-time extraction device, which comprises a device body, a device cover buckled on the device body, and an adding mechanism for adding experimental liquid into the device body; a cutting net is transversely arranged in the device body, and a space exists between the cutting net and an opening at the upper end of the device body; when the device cover is buckled on the device body, the lower end of the device cover positioned in the device body is in abutting contact with the upper end of the cutting net; the cell sieve is arranged below the cutting net in the device body, a circle of heating component is arranged on the periphery of the device body in a surrounding mode, the temperature in the device body is kept between 35 degrees and 39 degrees through the heating component, and the lower end of the device body is further connected with a rotating component driving the device body to rotate.
The main technical problems solved by the device are as follows: the placenta mesenchymal stem cell extraction device has the beneficial effects that the placenta mesenchymal stem cell extraction device provides the most suitable conditions for in-vitro separation and extraction of human amniotic mesenchymal stem cells, improves the extraction efficiency, has novel and simple structure, has high aggregation and reduces the infection risk; the placenta mesenchymal stem cells are efficiently treated by structural arrangement and effective arrangement design of a cutting net, a heating part, a cell sieve, a rotating assembly and a matched cylinder.
However, in the actual operation process, because the number of cells on a single group of test objects is too large, the accurate extraction cannot be performed, the cell concentration needs to be diluted at this time, the cell dilution liquid needs to be added in the extraction of the cells, and the rapid oscillation is needed after the cell dilution liquid is added, so that the cells and the cell dilution liquid are rapidly mixed, but the conventional cell dilution liquid adding equipment is often separated from the extraction equipment, so that the manual operation is required for many times, the inconvenience is caused, the manual oscillation is required after the cell dilution liquid is added, the working procedure is complex, and therefore, the placenta mesenchymal stem cell many times extraction device is provided.
Disclosure of Invention
The invention aims to provide a placenta mesenchymal stem cell multi-time extraction device so as to solve the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a placenta mesenchymal stem cell many times extraction element, includes test box, motor one, cell extraction equipment and diluent adding equipment, the inside fixedly connected with mount pad of test box, the surface mounting of mount pad has motor one, the internally mounted of mount pad has the installation axle and motor one's axis of rotation transmission connection, the one end of installation axle runs through the mount pad and rotates and connect the carousel, install mesenchymal stem cell on the carousel and wait to draw the mechanism;
the mesenchymal stem cell extraction mechanism comprises four groups of cell extraction control mechanisms, wherein the cell extraction control mechanisms comprise a fixed block, a second motor, a third motor and an electric telescopic rod, the fixed block is arranged in a mounting hole formed in the surface of a turntable, a fixed shaft is inserted into the fixed block, and one end of the fixed shaft is fixedly connected with a translation mechanism;
the translation mechanism comprises a mounting plate, a second motor and a first sliding block, wherein the surface of the mounting plate is fixedly connected with a connecting block through two groups of connecting rods, a bearing seat is fixedly connected to the inside of the connecting block, a screw rod is inserted into the bearing seat, the surface of the mounting plate is fixedly connected with the second motor, a rotating shaft of the second motor penetrates through the mounting plate to rotationally connect with the screw rod, the first sliding block is arranged on the surface of the screw rod, a threaded hole is formed in the center of the surface of the first sliding block, the screw rod is inserted into the threaded hole formed in the surface of the first sliding block, limiting holes are formed in two sides of the threaded hole on the surface of the first sliding block, and two groups of connecting rods are inserted into the limiting holes formed in the surface of the first sliding block;
the surface of the rotating mechanism is fixedly connected with an oscillating mechanism;
the surface of the vibration mechanism is fixedly connected with a test disc fixing mechanism;
the mesenchymal stem cell test disc is arranged in the test disc fixing mechanism and used for containing cells;
the surface of the top end of the test box body is provided with two groups of mounting grooves, and the two groups of mounting grooves are respectively and fixedly connected with cell extraction equipment and diluent adding equipment.
Preferably, the rotating mechanism comprises a supporting plate, a third motor, a control shaft and a rotating block, wherein the supporting plate is arranged on the surface of the first slider, the two groups of the supporting plates are fixedly connected with the surface of the first slider, the control shaft is inserted between the two groups of the supporting plates, the rotating block is fixedly connected with the surface of the control shaft, the third motor is fixedly connected with the surface of the supporting plate, the rotating shaft of the third motor is rotationally connected with the control shaft, and the oscillating mechanism is arranged on the surface of the rotating block.
Preferably, the second motor and the third motor are both servo motors.
Preferably, the vibration mechanism comprises a mounting box, a limiting rod, a second sliding block and an electric telescopic rod, wherein the mounting box is fixedly connected to the surface of a rotating block, the limiting rod is fixedly connected to the inside of the rotating block, the second sliding block is sleeved on the surface of the limiting rod, limiting springs are sleeved on the two sides of the second sliding block, one ends of the limiting springs are fixedly connected to the surface of the limiting rod and the other ends of the limiting springs are fixedly connected to the inner wall of the mounting box, a target block is fixedly connected to the surface of the second sliding block, an opening is formed in one side of the target block, an electric telescopic rod is fixedly connected to the opening formed in the surface of the mounting box, a pushing block and the pushing block are arranged on the electric telescopic rod in the mounting box in an inserted mode, the pushing block and the target block are located at the same horizontal height, and the test disc fixing mechanism is fixedly connected to the surface of the second sliding block.
Preferably, the test disc fixing mechanism comprises a mounting disc, a reset spring and a limit ball, wherein three groups of limit holes which are distributed circumferentially are formed in the inner wall of the mounting disc, the reset spring and the limit ball are all installed in the three groups of limit holes which are formed in the inner wall of the mounting disc, the limit ball is inserted into the limit hole which is formed in the inner wall of the mounting disc, one end of the reset spring is fixedly connected onto the inner wall of the limit hole which is formed in the mounting disc, the other end of the reset spring is fixedly connected onto the surface of the limit ball, and the mesenchymal stem cell test disc is inserted into the mounting disc.
Preferably, the mesenchymal stem cell test disc comprises a test disc body, a communication pipe and a baffle, wherein three groups of circumferentially distributed limiting holes are formed in the surface of the test disc body, the size of the limiting holes and the size of the limiting balls are matched with each other, openings and the communication pipe are fixedly connected to the surfaces of the two sides of the test disc body, and the baffle is fixedly connected to the surface of the top end of the test disc body.
Preferably, the inside of the test box body is positioned below the mesenchymal stem cell to-be-extracted mechanism and is fixedly connected with a waste liquid disc.
Preferably, the surface mounting of test box has the chamber door, be located the junction with the test box on the chamber door and set up sealed the pad, the surface of chamber door is provided with opening and opening part fixedly connected with glass.
Preferably, a controller is arranged on the surface of the test box body, and the controller is connected with the cell extraction equipment, the diluent adding equipment and the automatic equipment on the cell extraction control mechanism through control signals.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the scheme, through the arrangement of the mesenchymal stem cell to-be-extracted mechanism, cells can be contained in the mesenchymal stem cell test disc, the mesenchymal stem cell test disc is installed on the oscillating mechanism through the test disc fixing mechanism, meanwhile, the oscillating mechanism is installed on the translation mechanism through the rotating mechanism, the mesenchymal stem cell test disc can be rapidly displaced and replaced below the cell extraction equipment and the diluent adding equipment through the operation of the translation mechanism, so that the mesenchymal stem cell test disc can be added with cell diluent through the diluent adding equipment, then the cells are extracted through the cell extraction equipment, and in the process of adding the cell diluent into the diluent by the diluent adding equipment, the cell diluent is excessively added and can be rapidly moved to the lower part for re-addition;
2. according to the scheme, the middle mesenchymal stem cell test disc is arranged on the oscillation mechanism through the fixing mechanism, after the cell diluent is added, the cell diluent can be rapidly oscillated through the oscillation mechanism, so that the cell and the cell mixed liquid are rapidly mixed, and the cell extraction equipment is convenient to extract;
3. after adding the cell diluent through diluent adding equipment to the mesenchymal stem cell test panel, when the cell diluent is added too much, can drive through rotary mechanism and vibrate the mechanism rotation, make shake the fixed mesenchymal stem cell test panel of mechanism through test panel fixed establishment and rotate, further make the test panel body on the mesenchymal stem cell test panel can pour unnecessary cell and cell diluent mixture through the communicating pipe, and the mesenchymal stem cell is waited to draw the mechanism and is set up into four groups, go out the mesenchymal stem cell test panel of cell and cell diluent to the below through the mesenchymal stem cell test panel of top, make the mesenchymal stem cell test panel of top satisfy the requirement, make things convenient for cell extraction equipment to draw the cell, can debug once more in the mesenchymal stem cell test panel of below simultaneously, it is connected with the mesenchymal stem cell and wait to draw the mechanism to rotate through motor one operation drive, make the position of four groups of cell extraction control mechanism change, thereby the cell extraction control mechanism of below removes to the top and carries out cell extraction, the cell diluent of cell extraction control mechanism of the convenience, the use amount of cell dilution excessively has avoided the cell diluent of the while to cause inconvenient cell dilution.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of the structure of the present invention in section;
FIG. 3 is a schematic side cross-sectional view of the structure of the present invention;
FIG. 4 is a schematic diagram of a mechanism for extracting mesenchymal stem cells according to the present invention;
FIG. 5 is a schematic diagram showing the structure of a cell extraction control mechanism according to the present invention;
FIG. 6 is a schematic diagram showing the assembly of components of the cell extraction control mechanism of the present invention;
FIG. 7 is a schematic diagram of an oscillating mechanism according to the present invention;
FIG. 8 is a schematic cross-sectional view of the test disc fixing mechanism and the mesenchymal stem cell test disc structure of the present invention.
In the figure: 1. a test box; 2. a mounting base; 3. a first motor; 4. a mounting shaft; 5. a turntable; 6. a cell extraction control mechanism; 601. a fixed block; 602. a fixed shaft; 603. a mounting plate; 604. a connecting rod; 605. a connecting block; 606. a bearing seat; 607. a screw rod; 608. a second motor; 609. a first sliding block; 610. a support plate; 611. a third motor; 612. a control shaft; 613. a rotating block; 614. a mounting box; 615. a limit rod; 616. a second slide block; 617. a limit spring; 618. a target block; 619. an electric telescopic rod; 620. a pushing block; 621. a mounting plate; 622. a return spring; 623. a limit ball; 624. a test disc body; 625. a communicating pipe; 626. a baffle; 7. a cell extraction device; 8. a diluent adding device; 9. a waste liquid tray; 10. a door; 11. glass; 12. and a controller.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Referring to fig. 1-8, the present invention provides a technical solution:
the device adds mesenchymal stem cells into a group of cell extraction control mechanisms 6 on a mesenchymal stem cell extraction mechanism in a test box body 1, and then controls a box door 10 to seal and close the test box body 1 through a controller 12, so that the test box body 1 is in a sterile state;
the controller 12 controls the translation mechanism on the cell extraction control mechanism 6 to operate, the translation mechanism comprises a mounting plate 603, a motor II 608 and a slide block I609, the motor II 608 is mounted on the surface of the mounting plate 603, meanwhile, the same side surface of the mounting plate 603 is fixedly connected with a connecting block 605 through a release connecting rod 604, a mounting hole is formed in the connecting block 605, a bearing seat 606 is fixedly connected in the mounting hole, a screw rod 607 is inserted in the bearing seat 606, the screw rod 607 is positioned between the release connecting rods 604, a rotating shaft of the motor II 608 penetrates through the mounting plate 603 and the screw rod 607 to be rotationally connected, so that the screw rod 607 can be driven to rotate in the bearing seat 606 when the motor II 608 operates, the screw rod 607 is inserted in a threaded hole formed in the surface of the slide block I609, two groups of limiting holes are formed in the surface of the slide block I609 at the same time, the slide block I609 can slide on the surface of the screw rod 607 under the engagement of threads when the screw rod 607 rotates, and the motor II 608 is arranged as a servo motor, so that the motor II 608 can drive the screw rod 607 to rotate, and the slide in a reciprocating way on the surface 607 of the slide block I609;
after the screw rod 607 reciprocates, the mesenchymal stem cell test disc installed on the mesenchymal stem cell to-be-extracted mechanism can move, so that the mesenchymal stem cell test disc moves to the lower part of the cell extraction equipment 7 or the diluent adding equipment 8 installed above the inside of the test box body 1, the cell diluent can be added into the mesenchymal stem cell test disc through the diluent adding equipment 8, and the cells in the mesenchymal stem cell test disc can be extracted through the cell extraction equipment 7.
Example two
Referring to fig. 1-2, on the basis of the first embodiment, the present invention provides a technical solution:
the utility model provides a placenta mesenchymal stem cell multi-time extraction device, install rotary mechanism on the lead screw 607 on the mesenchymal stem cell waiting to draw the mechanism, rotary mechanism's surface mounting has vibration mechanism, vibration mechanism includes mounting box 614, gag lever post 615, slider two 616, spacing spring 617, electric telescopic rod 619 and ejector pad 620, the inside central point of mounting box 614 puts fixedly connected with gag lever post 615, slider two 616 is overlapped on the surface of gag lever post 615, slider two 616 is used for fixed connection test disc fixed establishment, the surface of gag lever post 615 is located the both sides of slider two 616 and all overlaps spacing spring 617 simultaneously, the one end of two sets of spacing springs 617 is all fixed connection on slider two 616 and the other end fixed connection is on the inner wall of mounting box 614, make slider two 616 in the surface sliding process of gag lever post 615, can extrude spacing spring 617 so that the elastic force of spacing spring 617 can cushion and reset the slip of slider two 616;
simultaneously, the surface of the second slider 616 is fixedly connected with a target block 618, the surface of the mounting box 614 is provided with an opening, the surface of the opening is fixedly connected with an electric telescopic rod 619, a push block 620 is mounted on the electric telescopic rod 619, the push block 620 penetrates through the opening formed in the surface of the mounting box 614 and is inserted into the mounting box 614, the push block 620 and the target block 618 keep the same horizontal height, the push block 620 is driven to slide forwards and retract fast through the electric telescopic rod 619, the push block 620 can push the second slider 616 to slide on the surface of the limiting rod 615, the second slider 616 can squeeze the limiting spring 617 and is rebounded by the limiting spring 617, the second slider 616 can slide back and forth on the limiting rod 615, further a test disc fixing mechanism mounted on the oscillating mechanism can vibrate back and forth, and further mesenchymal stem cells and cell diluents added into a mesenchymal stem cell test disc are mixed fast, and the cell extraction device 7 can extract cells more accurately and conveniently.
Example III
Referring to fig. 1-8, on the basis of the first embodiment and the second embodiment, the present invention provides a technical solution:
the utility model provides a placenta mesenchymal stem cell multiple extraction device, test panel fixed establishment includes mounting plate 621, reset spring 622 and spacing ball 623, the inner wall of mounting plate 621 is provided with three spacing hole equidistance and is the circumference release, reset spring 622 and spacing ball 623 are all installed in three spacing hole that the mounting plate 621 inner wall set up, spacing ball 623 is inserted in locating hole, reset spring 622's one end fixed connection is on the spacing hole inner wall that sets up in mounting plate 621, reset spring 622's the other end fixed connection is on spacing ball 623 surface, make spacing ball 623 can slide in the spacing hole that the mounting plate 621 surface set up, and spacing ball 623 can extrude reset spring 622 when receiving external force extrusion inwards to slide and compress, spacing ball 623 can be rebound under reset spring 622's elastic force after external force is withdrawn;
the mesenchymal stem cell test disc comprises a test disc body 624, a communication pipe 625 and a baffle 626, wherein three groups of limiting holes are formed in the surface of the test disc body 624, the sizes of three groups of limiting holes formed in the surface of the test disc body 624 are matched with the sizes of the limiting balls 623, the radius of the test disc body 624 is matched with the inner diameter of the mounting disc 621, the test disc body 624 can be quickly inserted into the mounting disc 621, the test disc body 624 can extrude the limiting balls 623 to slide inwards in the inserting process to compress the reset spring 622, after the test disc body 624 is inserted, the limiting balls 623 are reset under the elastic force of the reset spring 622 and are inserted into the limiting holes formed in the surface of the test disc body 624, the test disc body 624 is fixed in the mounting disc 621, quick mounting and taking are achieved, and the test disc body 624 can be more stable in the running process of the oscillating mechanism.
Example IV
Referring to fig. 1-8, on the basis of the first embodiment, the second embodiment and the third embodiment, the present invention provides a technical solution:
the utility model provides a placenta mesenchymal stem cell multiple extraction device, the inside fixedly connected with mount pad 2 of test box 1, the fixed surface of mount pad 2 is connected with motor one 3, the axis of rotation of motor one 3 rotates and connects installation axle 4, installation axle 4 inserts and locates in the mount pad 2 and the one end of installation axle 4 runs through mount pad 2 and rotates and connect carousel 5, mesenchymal stem cell waits to extract the mechanism and installs on carousel 5, mesenchymal stem cell waits to extract the mechanism and is constituteed by five sets of cell extraction control mechanism 6, every set of cell extraction control mechanism 6 all installs on carousel 5 through fixed block 601, fixed axle 602 is inserted to the inside of fixed block 601 and mounting panel 603 fixed connection is on fixed axle 602;
the first motor 3 drives the mounting shaft 4 to be connected with the rotary table 5 for rotation, so that four groups of cell extraction control mechanisms 6 mounted on the rotary table 5 can rotate along with the rotary table 5, thereby adjusting the positions of the four groups of cell extraction control mechanisms 6, and facilitating the operation of the four groups of cell extraction control mechanisms 6 by the cell extraction equipment 7 and the diluent adding equipment 8;
meanwhile, the four groups of cell extraction control mechanisms 6 can also rotate in the fixed block 601 through the fixed shaft 602 along with the rotating disc 5 in the rotating process, so that the cell extraction control mechanisms 6 always keep upwards no matter the cell extraction control mechanisms rotate to any position on the rotating disc 5, and the cells in the mesenchymal stem cell adaptation are prevented from falling.
Example five
Referring to fig. 1-8, on the basis of the fourth embodiment, the present invention provides a technical solution:
the placenta mesenchymal stem cell multi-time extraction device comprises a first slide block 609, a rotating mechanism fixedly connected with the first slide block 609, wherein the rotating mechanism comprises a support plate 610, a third motor 611, a control shaft 612 and a rotating block 613, two groups of support plates 610 are fixedly connected with the first slide block 609, the rotating block 613 is hinged between the two groups of support plates 610 through the control shaft 612, the bottom end surface of the rotating block 613 is fixedly connected with a mounting box 614 on an oscillating mechanism, meanwhile, the third motor 611 is mounted on the surface of the support plate 610, the rotating shaft of the third motor 611 is rotationally connected with the control shaft 612, the rotating block 613 can be driven by the third motor 611 to drive the oscillating mechanism to rotate, so that a test disc fixing mechanism mounted on the oscillating mechanism and a mesenchymal stem cell test disc can synchronously rotate, and the third motor 611 is arranged as a servo motor, and the rotating direction of the rotating block 613 can be controlled;
the openings are formed in the two side surfaces of the test disc body 624, the communication pipes 625 are fixedly connected to the surfaces of the openings, so that cells and cell diluent in the test disc body 624 can be poured out through the communication pipes 625 in the rotation process of the test disc body 624, the communication pipes 625 are bent upwards, excessive leakage of the cell diluent is avoided, the cell diluent can flow into a mesenchymal stem cell test disc below when discharged conveniently, and meanwhile, the cells and the cell diluent can be prevented from being poured out in the vibration process and the turnover process of the mesenchymal stem cell test disc by the baffle 626 fixedly connected to the surfaces of the test disc body 624;
when a group of cell extraction control mechanisms 6 rotate to the uppermost position on the turntable 5, cell diluent is added into the interphase mesenchymal stem cell test disc through diluent adding equipment 8, when the adding amount of the cell diluent is small, repeated adding can be carried out through the diluent adding equipment 8, and debugging is carried out through the oscillating mechanism, when the adding amount of the cell diluent is large, the rotation of the mesenchymal stem cell test disc is controlled through the rotating mechanism, so that cells in a test disc body 624 in the uppermost mesenchymal stem cell test disc and the cell diluent can be led into the cell extraction control mechanism 6 below through the test disc body 624, the mixture of the cells and the cell diluent is diluted again, and the convenience of cell extraction of the cell extraction equipment 7 is improved;
and through the setting of multiunit cell extraction control mechanism 6 for this device is carried out many times cell diluent and is added and dilute the back, can carry out many times and draw, improves the extraction quantity of this test alone.
Example six
Referring to fig. 1-8, on the basis of the fifth embodiment, the present invention provides a technical solution:
the placenta mesenchymal stem cell multi-time extraction device is characterized in that a waste liquid disc 9 is arranged below a mesenchymal stem cell to-be-extracted mechanism in the test box body 1, and redundant cells and cell diluent mixtures in the four groups of cell extraction control mechanisms 6 can be poured down through the waste liquid disc 9, so that the collection is convenient;
meanwhile, the surface of the test box body 1 is provided with an opening, the bayonet is provided with a box door 10, the test box body 1 can be sealed through the box door 10 to prevent bacterial infection, the surface of the box door 10 is provided with an opening, the opening is provided with glass 11, and the test box body 1 can be observed after being sealed through the glass 11;
the surface of the test box body 1 is fixedly connected with a controller 12, and various automatic apparatuses in the device can be controlled to be switched through the controller 12, such as a cell extraction device 7, a diluent adding device 8, a motor, an electric telescopic rod and the like.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation. The term "comprising" an element defined by the term "comprising" does not exclude the presence of other identical elements in a process, method, article or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. The utility model provides a placenta mesenchymal stem cell extraction element many times, includes test box (1), motor one (3), cell extraction equipment (7) and diluent adding equipment (8), its characterized in that: the device comprises a test box body (1), wherein an installation seat (2) is fixedly connected to the inside of the test box body (1), a motor I (3) is arranged on the surface of the installation seat (2), an installation shaft (4) is arranged in the installation seat (2), the installation shaft (4) is in transmission connection with a rotating shaft of the motor I (3), one end of the installation shaft (4) penetrates through the installation seat (2) to be connected with a rotating disc (5) in a rotating manner, and a mesenchymal stem cell extraction mechanism is arranged on the rotating disc (5);
the mesenchymal stem cell extraction mechanism comprises four groups of cell extraction control mechanisms (6), wherein the cell extraction control mechanisms (6) comprise a fixed block (601), a second motor (608), a third motor (611) and an electric telescopic rod (619), the fixed block (601) is arranged in a mounting hole formed in the surface of a turntable (5), a fixed shaft (602) is inserted into the fixed block (601), and one end of the fixed shaft (602) is fixedly connected with a translation mechanism;
the translation mechanism comprises a mounting plate (603), a motor II (608) and a slider I (609), wherein the surface of the mounting plate (603) is fixedly connected with a connecting block (605) through two groups of connecting rods (604), a bearing seat (606) is fixedly connected to the inside of the connecting block (605), a screw rod (607) is inserted into the bearing seat (606), the surface of the mounting plate (603) is fixedly connected with the motor II (608), a rotating shaft of the motor II (608) penetrates through the mounting plate (603) to be rotationally connected with the screw rod (607), a slider I (609) is arranged on the surface of the screw rod (607), a threaded hole is formed in the center of the surface of the slider I (609), limiting holes are formed in the two sides of the threaded hole on the surface of the slider I (609), and two groups of connecting rods (604) are inserted into the limiting holes formed in the surface of the slider I (609) respectively;
the surface of the rotating mechanism is fixedly connected with an oscillating mechanism;
the surface of the vibration mechanism is fixedly connected with a test disc fixing mechanism;
the mesenchymal stem cell test disc is arranged in the test disc fixing mechanism and used for containing cells;
two groups of mounting grooves are formed in the top surface of the test box body (1), and cell extraction equipment (7) and diluent adding equipment (8) are fixedly connected to the two groups of mounting grooves respectively;
the rotating mechanism comprises a supporting plate (610), a motor III (611), a control shaft (612) and a rotating block (613), wherein the supporting plate (610) is arranged into two groups which are fixedly connected to the surface of a first sliding block (609), the control shaft (612) is inserted between the two groups of the supporting plates (610), the rotating block (613) is fixedly connected to the surface of the control shaft (612), the motor III (611) is fixedly connected to the surface of one group of the supporting plates (610), the rotating shaft of the motor III (611) is rotationally connected with the control shaft (612), and the oscillating mechanism is arranged on the surface of the rotating block (613);
the vibration mechanism comprises a mounting box (614), a limit rod (615), a second slider (616) and an electric telescopic rod (619), wherein the mounting box (614) is fixedly connected to the surface of a rotating block (613), the limit rod (615) is fixedly connected to one side of the rotating block (613), the second slider (616) is sleeved on the surface of the limit rod (615), limit springs (617) are sleeved on the two sides of the second slider (616) on the surface of the limit rod (615), one ends of the two limit springs (617) are fixedly connected to the surface of the limit rod (615) and the other ends of the two limit springs are fixedly connected to the inner wall of the mounting box (614), a target block (618) is fixedly connected to the surface of the second slider (616), an electric telescopic rod (619) is fixedly connected to the opening formed in the surface of the mounting box (614), a push block (620) is mounted on the electric telescopic rod (619), the push block (620) is arranged in the mounting box (614) in a plug-in mode, and the push block (620) and the two push block (618) are fixedly connected to the same horizontal disc (616);
the test disc fixing mechanism comprises a mounting disc (621), a reset spring (622) and a limit ball (623), wherein three groups of limit holes which are distributed circumferentially are formed in the inner wall of the mounting disc (621), the reset spring (622) and the limit ball (623) are all installed in the three groups of limit holes formed in the inner wall of the mounting disc (621), the limit ball (623) is inserted into the limit hole formed in the inner wall of the mounting disc (621), one end of the reset spring (622) is fixedly connected to the inner wall of the limit hole formed in the mounting disc (621), the other end of the reset spring (622) is fixedly connected to the surface of the limit ball (623), and the mesenchymal stem cell test disc is inserted into the mounting disc (621);
the mesenchymal stem cell test disc comprises a test disc body (624), a communication pipe (625) and a baffle (626), wherein three groups of limit holes distributed circumferentially are formed in the surface of the test disc body (624), the size of the limit holes formed in the surface of the test disc body (624) is matched with the size of the limit balls (623), openings and fixedly connected with the communication pipe (625) are formed in the surfaces of the two sides of the test disc body (624), and the baffle (626) is fixedly connected to the surface of the top end of the test disc body (624).
2. The multiple extraction device for placental mesenchymal stem cells according to claim 1, wherein: and the second motor (608) and the third motor (611) are both arranged as servo motors.
3. The multiple extraction device for placental mesenchymal stem cells according to claim 1, wherein: the inside of the test box body (1) is positioned below the mesenchymal stem cell to-be-extracted mechanism and is fixedly connected with a waste liquid disc (9).
4. The multiple extraction device for placental mesenchymal stem cells according to claim 3, wherein: the surface mounting of test box (1) has chamber door (10), be located on chamber door (10) and set up sealed pad with the junction of test box (1), the surface of chamber door (10) is provided with opening and opening part fixedly connected with glass (11).
5. The multiple extraction device of placental mesenchymal stem cells of claim 4, wherein: the surface of the test box body (1) is provided with a controller (12), and the controller (12) is connected with the cell extraction equipment (7), the diluent adding equipment (8) and the automatic equipment control signals on the cell extraction control mechanism (6).
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