CN110846227B

CN110846227B - Stem cell culture device

Info

Publication number: CN110846227B
Application number: CN201911205021.6A
Authority: CN
Inventors: 王冬
Original assignee: Anhui Guozhen Biotechnology Co ltd
Current assignee: Anhui Guozhen Biotechnology Co ltd
Priority date: 2019-11-29
Filing date: 2019-11-29
Publication date: 2023-01-31
Anticipated expiration: 2039-11-29
Also published as: CN110846227A

Abstract

The invention discloses a stem cell culture device which comprises a cell tissue crushing and washing module, a PBS (phosphate buffer solution) recycling and transmitting module, a PBS transferring module, a cell tissue oscillating module and a tissue filtering and transferring module. The cell tissue crushing and washing module comprises a first lead screw, a first motor, a second motor, a stirring rod, a stirring head, a lifting seat and a crushing and washing tank; the PBS recycling transmission module comprises a transmission belt and a PBS storage tank; the PBS transfer module comprises a second lead screw, a third motor, a fourth motor, a third lead screw, a transverse column and a transfer plate; the cell tissue oscillation module comprises a fifth motor, a first rotating shaft, an oscillation tank, a second rotating shaft and a rotating block; the tissue filtering and transferring module comprises a cylinder, a rotary table and a filter screen. The invention has simple structure and strong practicability, liberates the hands of scientific research personnel and increases the culture efficiency of stem cells. Solves the problems of manually preparing culture solution and manually culturing and transferring stem cells in the prior art.

Description

Stem cell culture device

Technical Field

The invention belongs to the field of cell culture, and particularly relates to a stem cell culture device.

Background

With the continuous development of the current science and technology, the application of cell engineering is very wide, the cell engineering has obvious effect in both medical and pharmaceutical fields, and the cell culture in the cell engineering is very important as the basis of the whole engineering.

Stem cells are a type of pluripotent cells that are capable of self-replication. Under certain conditions, it can differentiate into multiple functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. Stem cells are insufficiently differentiated and immature cells, have potential functions of regenerating various tissues, organs and human bodies, and are called universal cells in the medical field. Therefore, the culture of stem cells is of great importance.

Most of traditional stem cell culture is that culture solution is prepared manually by scientific research personnel, and stem cell culture transfer is carried out manually, so that the method wastes time and labor, and the working efficiency is low. Aiming at the situations, a stem cell culture device is designed to realize the automatic culture of stem cells.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a stem cell culture device, which solves the problems that culture solution is manually prepared and stem cells are manually cultured and transferred in the prior art.

The purpose of the invention can be realized by the following technical scheme:

the utility model provides a stem cell culture device, includes that cell tissue smashes and washes module, PBS and recycles transport module, PBS and transports module, cell tissue oscillation module and tissue filtration transfer module, cell tissue smashes and washes module and PBS and transport the module and place the both ends that PBS recycled transport module respectively in, cell tissue oscillation module places the one end that PBS transported the module in, tissue filtration transfer module places the cell tissue oscillation module and PBS and recycles between the transport module.

Cell tissue stirring washing module includes stand and first lead screw, be equipped with the second motor on the stand, the lower extreme of stand is equipped with the dwang, the output intercommunication stand of second motor rotates with the dwang to be connected.

The PBS transfer module comprises a second lead screw, a nut and a cross column are fixedly connected on the second lead screw, a fourth motor is arranged on the cross column, a third lead screw is arranged in the cross column, the output end of the fourth motor is communicated with the cross column and is in transmission connection with the third lead screw, and the third lead screw is in threaded fit with the transfer plate.

The cell tissue oscillation module includes fifth motor and oscillation tank, the output of fifth motor and the one end fixed connection of first pivot, the other end and the square post of first pivot rotate to be connected, oscillation tank's side is equipped with the fixed plate, be equipped with the second pivot in the fixed plate, the turning block normal running fit of second pivot and, the turning block is run through to first pivot.

The tissue filtering and transferring module comprises a cylinder, a first connecting rod is fixedly arranged at the side end of the cylinder, a rotary table is rotatably connected to one end of the first connecting rod, and the rotary table and the filter screen are fixedly connected through a second connecting rod.

Cell tissue pulped washing module is including supporting the base, be equipped with first base on the support base, the one end of first base is equipped with first motor, the output intercommunication first base of first motor and with first lead screw transmission be connected.

The lower extreme of dwang is fixed and is equipped with the disc, the fixed puddler that is equipped with stirring head and array distribution of lower extreme of disc.

The stirring device is characterized in that a stirring flushing tank arranged on the lifting seat is arranged under the stirring head, a sliding block is arranged at one end of the lifting seat, the sliding block is in threaded fit with a first lead screw, a first blanking pipeline penetrating through the lifting seat is arranged at the lower end of the stirring flushing tank, and a first electromagnetic valve is arranged on the first blanking pipeline.

PBS recycles transfer module includes spill post and conveyer belt, spill post and conveyer belt rotate to be connected, be equipped with the PBS holding vessel on the conveyer belt, the lower extreme of PBS holding vessel is equipped with second unloading pipeline, be equipped with the second solenoid valve on the second unloading pipeline.

The PBS transfer module comprises a second base, a third motor is arranged at one end of the second base, and the output end of the third motor is communicated with the second base and is in transmission connection with a second lead screw.

The cell tissue oscillation module comprises a third base, a fourth base is arranged on the third base, and a fifth motor is arranged on the fourth base.

The side end of the rotating block is provided with arc-shaped notches which are distributed in an array manner.

The tissue filtering and transferring module comprises a fifth base, and a cylinder in rotary connection is vertically arranged on the fifth base.

The filter screen is arranged under the first blanking pipeline and arranged over the PBS storage tank, and the cylinder is arranged over the oscillation tank through rotation.

The invention has the beneficial effects that:

1. the PBS recycling and transmitting module is arranged, so that the PBS can be recycled, materials are saved, and the cost is reduced;

2. the invention is provided with a cell tissue oscillation module which can control the vibration of the whole module in the vertical direction through the rotation in the horizontal direction;

3. the invention has simple structure and strong practicability, liberates the hands of scientific research personnel and increases the culture efficiency of stem cells.

Drawings

In order to more clearly illustrate the embodiments or prior art solutions of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic diagram of the overall structure of an embodiment of the present invention;

FIG. 2 is a schematic diagram of a cell tissue mincing and washing module according to an embodiment of the invention;

FIG. 3 is a schematic diagram of a PBS reuse transport module according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of the bottom structure of a PBS recycling transport module according to an embodiment of the invention;

FIG. 5 is a schematic view of a PBS transport module according to an embodiment of the present invention;

FIG. 6 is a bottom view of a PBS transport module according to an embodiment of the present invention;

FIG. 7 is a schematic view of a tissue oscillation module according to an embodiment of the present invention;

FIG. 8 is a front view of a tissue oscillation module according to an embodiment of the present invention;

FIG. 9 isbase:Sub>A sectional view taken along line A-A of FIG. 8;

fig. 10 is a tissue filtration transfer module of an embodiment of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1, a stem cell culture apparatus comprises five parts, namely a cell tissue mincing and washing module 1, a PBS recycling and conveying module 2, a PBS transferring module 3, a cell tissue oscillating module 4 and a tissue filtering and transferring module 5, wherein the cell tissue mincing and washing module 1 and the PBS transferring module 3 are respectively arranged at two ends of the PBS recycling and conveying module 2, the cell tissue oscillating module 4 is arranged at one end of the PBS transferring module 3, and the tissue filtering and transferring module 5 is arranged between the cell tissue oscillating module 4 and the PBS recycling and conveying module 2.

As shown in fig. 2, the cell tissue mincing and washing module 1 includes a supporting base 11, a first base 12 and a column 15 are arranged on the supporting base 11, a first lead screw 13 is vertically arranged on the first base 12, a first motor 14 is arranged at one end of the first base 12, and an output end of the first motor 14 is communicated with the first base 12 and is in transmission connection with the first lead screw 13. Be equipped with second motor 111 on the stand 15, the lower extreme of stand 15 is equipped with dwang 112, the output intercommunication stand 15 of second motor 111 and with dwang 112 rotation be connected. The lower end of the rotating rod 112 is fixedly provided with a disc 113, and the lower end of the disc 113 is fixedly provided with a stirring head 114 and stirring rods 115 distributed in an array manner. A stirring and washing tank 18 arranged on the lifting seat 16 is arranged right below the stirring head 114, a sliding block 17 is arranged at one end of the lifting seat 16, and the sliding block 17 is in threaded fit with the first lead screw 13. The lower end of the mashing and washing tank 18 is provided with a first blanking pipeline 19 penetrating through the lifting seat 16, and the first blanking pipeline 19 is provided with a first electromagnetic valve 110.

As shown in fig. 3, the PBS recycling and conveying module 2 includes a concave column 21 and a conveyor belt 22, the concave column 21 is rotatably connected to the conveyor belt 22, a PBS storage tank 23 is disposed on the conveyor belt 22, as shown in fig. 4, a second discharging pipe 24 is disposed at a lower end of the PBS storage tank 23, and a second electromagnetic valve 25 is disposed on the second discharging pipe 24.

As shown in fig. 5, the PBS transferring module 3 includes a second base 31, a second lead screw 32 is vertically disposed on the second base 31, a third motor 33 is disposed at one end of the second base 31, and an output end of the third motor 33 is communicated with the second base 31 and is in transmission connection with the second lead screw 32. The nut on the second screw rod 32 is fixedly connected with the cross column 34, the cross column 34 is provided with a fourth motor 35, as can be seen from fig. 5 and 6, the cross column 34 is provided with a third screw rod 37, and the output end of the fourth motor 35 is communicated with the cross column 34 and is in transmission connection with the third screw rod 37. The third lead screw 37 is screwed with the transfer plate 36.

As shown in fig. 7, the cell tissue oscillation module 4 includes a third base 41, and a fourth base 42 and a square column 43 are disposed on the third base 41. Be equipped with fifth motor 46 on the fourth base 42, the output of fifth motor 46 and the one end fixed connection of first pivot 47, the other end and the square post 43 of first pivot 47 rotate to be connected. As can be seen from fig. 7, 8, and 9, an oscillating tank 44 is disposed above the third base 41, a fixing plate 45 is disposed at a side end of the oscillating tank 44, a second rotating shaft 49 is disposed in the fixing plate 45, the second rotating shaft 49 is attached to a rotating block 48 having arc notches distributed in an array at a side end, and the first rotating shaft 47 penetrates through the rotating block 48. It should be noted that the second rotating shaft 49 is rotatably engaged with the rotating block 48.

As shown in fig. 10, the tissue filtering and transferring module 5 includes a fifth base 51, a cylinder 52 is vertically disposed on the fifth base 51, a first connecting rod 53 is fixedly disposed at a side end of the cylinder 52, a rotating table 54 is rotatably connected to one end of the first connecting rod 53, and the rotating table 54 is fixedly connected to a filter screen 56 through a second connecting rod 55. It should be noted that the filter screen 56 is disposed right below the first blanking pipe 19 and right above the PBS storage tank 23, and the cylinder 52 is rotated 180 ° and then disposed right above the oscillation tank 44.

When the cell tissue and fresh PBS are used, the cell tissue and fresh PBS are firstly placed into the mashing and washing tank 18, then the first motor 14 is started, the first lead screw 13 is controlled to rotate, the lifting seat 16 is further enabled to move upwards, and the cell tissue and fresh PBS stop after the stirring head 114 and the stirring rod 115 are placed in the mashing and washing tank 18 for a certain depth. Then, the second motor 111 is started, so that the rotating rod 112 drives the stirring head 114 and the stirring rod 115 to rotate, and the cell tissue is smashed and washed by the PBS. After the mincing and washing is completed, the first motor 14 is reversed to lower the lifting base 16 to a certain height and then stopped, and the first solenoid valve 110 is opened to allow the cell tissue fragments and the PBS to be poured from the mincing and washing tank 18 onto the filtering net 56.

Through the filtration of filter screen 56, the cell tissue fragment is placed on filter screen 56, PBS falls into PBS holding vessel 23 of below, then rotate cylinder 52, make filter screen 56 place directly over oscillation tank 44, then 180 rotate revolving stage 54, make the cell tissue fragment fall into to oscillation tank 44, go into normal saline and culture solution to filter screen 56, make remaining cell tissue fragment on filter screen 56 fall into oscillation tank 44 together with normal saline and culture solution, start fifth motor 46 after that, make first pivot 47 drive turning block 48 and rotate, and then drive second pivot 49 and rotate, finally realize oscillation tank 44 and vibrate from top to bottom, stop fifth motor 46 after the oscillation is accomplished.

Then, the conveyor belt 22 is started to convey the PBS storage tank with PBS onto the transfer plate 36, and then the third motor 33 is started to rotate the second lead screw 32, so as to drive the cross column 34 and the transfer plate 36 to move upwards, and stop when the PBS storage tank rises to a certain height. Then start fourth motor 35 for third lead screw 37 rotates, and then drives transfer board 36 horizontal motion, stops after transfer board 36 moves directly over oscillation tank 44, then opens second solenoid valve 25 for PBS falls into oscillation tank 44 by second unloading pipeline 24, carries out the secondary to the cell tissue and washes, takes out the cell tissue after washing the completion, changes into 37 degrees centigrade CO after the centrifugation ₂ Culturing in an incubator for a period of time to obtain stem cells.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing shows and describes the general principles, principal features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.

Claims

1. A stem cell culture device comprises a cell tissue mincing and washing module (1), a PBS (phosphate buffer solution) recycling and conveying module (2), a PBS (phosphate buffer solution) transferring module (3), a cell tissue oscillating module (4) and a tissue filtering and transferring module (5), and is characterized in that the cell tissue mincing and washing module (1) and the PBS (phosphate buffer solution) transferring module (3) are respectively arranged at two ends of the PBS recycling and conveying module (2), the cell tissue oscillating module (4) is arranged at one end of the PBS (phosphate buffer solution) transferring module (3), and the tissue filtering and transferring module (5) is arranged between the cell tissue oscillating module (4) and the PBS recycling and conveying module (2);

the cell tissue smashing and washing module (1) comprises an upright post (15) and a first lead screw (13), a second motor (111) is arranged on the upright post (15), a rotating rod (112) is arranged at the lower end of the upright post (15), and the output end of the second motor (111) is communicated with the upright post (15) and is rotatably connected with the rotating rod (112);

the PBS transfer module (3) comprises a second lead screw (32), a nut on the second lead screw (32) is fixedly connected with a transverse column (34), a fourth motor (35) is arranged on the transverse column (34), a third lead screw (37) is arranged in the transverse column (34), the output end of the fourth motor (35) is communicated with the transverse column (34) and is in transmission connection with the third lead screw (37), and the third lead screw (37) is in threaded fit with a transfer plate (36);

the cell tissue oscillation module (4) comprises a fifth motor (46) and an oscillation tank (44), the output end of the fifth motor (46) is fixedly connected with one end of a first rotating shaft (47), the other end of the first rotating shaft (47) is rotatably connected with a square column (43), a fixing plate (45) is arranged at the side end of the oscillation tank (44), a second rotating shaft (49) is arranged in the fixing plate (45), the second rotating shaft (49) is rotatably matched with a rotating block (48), and the first rotating shaft (47) penetrates through the rotating block (48);

the tissue filtering and transferring module (5) comprises a cylinder (52), a first connecting rod (53) is fixedly arranged at the side end of the cylinder (52), a rotary table (54) is rotatably connected to one end of the first connecting rod (53), and the rotary table (54) is fixedly connected with a filter screen (56) through a second connecting rod (55).

2. The stem cell culture device according to claim 1, wherein the cell tissue mincing and washing module (1) comprises a support base (11), a first base (12) is arranged on the support base (11), a first motor (14) is arranged at one end of the first base (12), and an output end of the first motor (14) is communicated with the first base (12) and is in transmission connection with a first lead screw (13).

3. The stem cell culture device according to claim 1, wherein a disc (113) is fixedly arranged at the lower end of the rotating rod (112), and a stirring head (114) and stirring rods (115) distributed in an array are fixedly arranged at the lower end of the disc (113).

4. The stem cell culture device according to claim 3, wherein a stirring washing tank (18) arranged on a lifting seat (16) is arranged right below the stirring head (114), a sliding block (17) is arranged at one end of the lifting seat (16), the sliding block (17) is in threaded fit with the first lead screw (13), a first blanking pipeline (19) penetrating through the lifting seat (16) is arranged at the lower end of the stirring washing tank (18), and a first electromagnetic valve (110) is arranged on the first blanking pipeline (19).

5. The stem cell culture device according to claim 1, wherein the PBS recycling transmission module (2) comprises a concave column (21) and a transmission belt (22), the concave column (21) is rotatably connected with the transmission belt (22), a PBS storage tank (23) is arranged on the transmission belt (22), a second blanking pipeline (24) is arranged at the lower end of the PBS storage tank (23), and a second electromagnetic valve (25) is arranged on the second blanking pipeline (24).

6. The stem cell culture device according to claim 1, wherein the PBS transfer module (3) comprises a second base (31), one end of the second base (31) is provided with a third motor (33), and the output end of the third motor (33) is communicated with the second base (31) and is in transmission connection with a second lead screw (32).

7. The stem cell culture device according to claim 1, wherein the cell tissue shaking module (4) comprises a third base (41), a fourth base (42) is arranged on the third base (41), and a fifth motor (46) is arranged on the fourth base (42).

8. The stem cell culture device according to claim 1, wherein the side ends of the rotating block (48) are provided with arc-shaped notches distributed in an array.

9. The stem cell culture device according to claim 1, wherein the tissue filtering and transferring module (5) comprises a fifth base (51), and a rotatably connected cylinder (52) is vertically arranged on the fifth base (51).

10. The stem cell culture apparatus according to claim 1, wherein the filter screen (56) is disposed directly below the first blanking line (19) and directly above the PBS storage tank (23), and the cylinder (52) is disposed directly above the shaking tank (44) by rotation.