CN214011057U - Underwater multi-wavelength backscatter and fluorescence monitoring probe - Google Patents
Underwater multi-wavelength backscatter and fluorescence monitoring probe Download PDFInfo
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- CN214011057U CN214011057U CN202021620009.XU CN202021620009U CN214011057U CN 214011057 U CN214011057 U CN 214011057U CN 202021620009 U CN202021620009 U CN 202021620009U CN 214011057 U CN214011057 U CN 214011057U
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Abstract
The embodiment of the application discloses probe is monitored with fluorescence to multi-wavelength backscatter under water, its characterized in that includes: the device comprises a sealed cabin shell, a first excitation light source (1), a second excitation light source (2), a third excitation light source (3), a first photoelectric detector (4), a second photoelectric detector (5), a third photoelectric detector (6) and a constant current source driving modulation circuit, wherein the first excitation light source, the second excitation light source, the third excitation light source, the first photoelectric detector, the second photoelectric detector and the third photoelectric detector are positioned in the sealed cabin shell; the central wavelengths of the first excitation light source (1), the second excitation light source (2) and the third excitation light source (3) are different; the probe further comprises: the photoelectric detector comprises a first band-pass filter positioned at the front end of the first photoelectric detector (4), a second band-pass filter positioned at the front end of the second photoelectric detector (5), and a third band-pass filter positioned at the front end of the third photoelectric detector (6). The probe can realize the miniaturization, low power consumption, multi-wavelength and integrated detection of the underwater in-situ backscattering coefficient and the chlorophyll concentration.
Description
Technical Field
The application relates to the technical field of optical in-situ detection, in particular to an underwater multi-wavelength backscattering and fluorescence monitoring probe device.
Background
The water body backscattering coefficient is an important ocean optical parameter and plays an important role in ocean water color remote sensing, water color component concentration inversion and a biological optical model. The distribution condition of phytoplankton and the distribution change rule of primary organic matters can be known by researching the concentration of chlorophyll in the ocean, and the early warning effect on red tide caused by water eutrophication is achieved. Many foreign companies develop a plurality of mature ocean optical sensors, and the ocean optical sensors are widely applied to aspects of ocean environmental investigation, ecological environment monitoring, red tide monitoring and the like. However, most of the research in the field of China still stays in the experimental prototype stage, the market of the domestic marine monitoring instrument is basically monopolized abroad, and the development of the technology and equipment with independent intellectual property rights is urgent.
In marine observation, in traditional field sampling and laboratory analysis, the sample changes its relevant characteristics due to changes in temperature, pressure and light during collection, transportation and storage. Meanwhile, the field record of an emergency or a severe environment cannot be obtained, so that the development of the in-situ sensor capable of carrying out long-time field measurement is of great significance. The optical method gradually becomes the mainstream of the biochemical detection method due to the advantages of high stability, difficult pollution, no need of regular calibration and the like. The optical method mainly comprises a spectrophotometry, a fluorescence method and a scattering light method, wherein the fluorescence method and the scattering light method have higher measurement precision at low concentration of substances, are generally 1-2 orders higher than the spectrophotometry, have good stability and are widely applied to in-situ low-concentration substance measurement. At present, various sensors are developed in the multi-band, miniaturized, low power consumption, high stability and in-situ multi-parameter detection direction.
Based on the research background, the underwater multi-wavelength backscattering and fluorescence monitoring probe is a multi-band integrated marine optical sensor and adopts the principle that a stable light emitting diode is used as an excitation light source, a high-sensitivity photoelectric detector is used for receiving backscattering and fluorescence signals, and a working mode of light source time-sharing emission and photoelectric detector multiplexing is adopted in the detection process, so that the interference of environment stray light can be effectively avoided, the system volume and the power consumption are reduced, and the long-term, continuous and real-time observation application of marine environment can be realized.
Disclosure of Invention
The embodiment of the application provides an underwater multi-wavelength backscattering and fluorescence monitoring probe, and the underwater multi-wavelength backscattering and fluorescence monitoring probe has the characteristics of miniaturization, low power consumption and integration.
In a first aspect, an embodiment of the present application provides an underwater multi-wavelength backscatter and fluorescence monitoring probe, which includes: the device comprises a sealed cabin shell, a first excitation light source (1), a second excitation light source (2), a third excitation light source (3), a first photoelectric detector (4), a second photoelectric detector (5), a third photoelectric detector (6) and a constant current source driving modulation circuit, wherein the first excitation light source, the second excitation light source, the third excitation light source, the first photoelectric detector, the second photoelectric detector and the third photoelectric detector are positioned in the sealed cabin shell; the central wavelengths of the first excitation light source (1), the second excitation light source (2) and the third excitation light source (3) are different; the probe further comprises: the first band-pass filter is positioned at the front end of the first photoelectric detector (4), the second band-pass filter is positioned at the front end of the second photoelectric detector (5), and the third band-pass filter is positioned at the front end of the third photoelectric detector (6), wherein the band-pass center wavelength of the first band-pass filter is the same as the center wavelength of the first excitation light source (1), and the band-pass center wavelength of the second band-pass filter is the same as the center wavelength of the second excitation light source (2); the center wavelength of the band-pass of the third band-pass filter is the same as the center wavelength of the third excitation light source (3); the first photoelectric detector (4) is used for detecting a backscattering signal of the first excitation light source (1) at 117 degrees; the second photoelectric detector (4) is used for detecting a backscattering signal of the second excitation light source (2) at 117 degrees, and the second photoelectric detector (5) is also used for detecting a chlorophyll fluorescence signal of the third excitation light source (3) at 142 degrees; the third photoelectric detector (6) is used for detecting a backscattering signal of the third excitation light source (3) at 142 degrees; the constant current source driving modulation circuit is used for controlling only the first excitation light source (1) and the second excitation light source (2) to emit light in a first time period, the driving circuit is also used for controlling only the third excitation light source (3) to emit light in a second time period, and no overlapping part exists between the first time period and the second time period.
With reference to the first aspect, in a first possible implementation manner of the first aspect, the first excitation light source (1) is a light emitting diode with a central wavelength of 550nm, the second excitation light source (2) is a light emitting diode with a central wavelength of 680nm, the third excitation light source (3) is a light emitting diode with a central wavelength of 450nm, the central wavelength of the first bandpass filter is 550nm, the central wavelength of the second bandpass filter is 680nm, and the central wavelength of the third bandpass filter is 450 nm.
With reference to the first aspect and the foregoing implementation manner, in a second possible implementation manner of the first aspect, the constant current source driving modulation circuit uses a constant current source driving circuit to high-frequency modulate the first excitation light source (1), the second excitation light source (2), and the third excitation light source (3).
With reference to the first aspect and the foregoing implementation manner of the first aspect, in a third possible implementation manner of the first aspect, the probe further includes: a first optical window, a second optical window, a third optical window, a fourth optical window, a fifth optical window, and a sixth optical window, the first optical window is placed in front of the first excitation light source (1), the second optical window is placed in front of the second excitation light source (2), the third optical window is placed in front of the third excitation light source (3), the fourth optical window is placed in front of the first bandpass filter, the fifth optical window is placed before the second bandpass filter, the sixth optical window is placed before the third bandpass filter, the first optical window, the second optical window, the third optical window, the fourth optical window, the fifth optical window and the sixth optical window are all arranged in parallel with the front end cover face of the probe, and the optical windows are made of quartz glass or sapphire.
With reference to the first aspect and the foregoing implementation manner of the first aspect, in a fourth possible implementation manner of the first aspect, the probe further includes: and the watertight plug is positioned on the sealed cabin shell.
Compared with the prior art, the utility model is characterized in that:
(1) the constant current source driving circuit is adopted to modulate the light source at high frequency, so that the anti-interference capability of the ambient light is strong, and the practical value is important.
(2) The working mode of light source time-sharing emission and photoelectric detector multiplexing is adopted, so that multi-wavelength integrated detection of backscattering and chlorophyll fluorescence signals is realized;
(3) the system has simpler light path and lower power consumption, is beneficial to miniaturization and integrated detection, and can carry various carrying platforms.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 shows a schematic layout of a probe head cover according to an embodiment of the present application.
Fig. 2 shows a schematic structural diagram of a probe according to an embodiment of the present application, wherein a shows a schematic structural diagram of a cross section of a probe according to an embodiment of the present application, b shows a schematic structural diagram of a cross section of a probe according to another embodiment of the present application, and c shows a schematic structural diagram of a cross section of a probe according to yet another embodiment of the present application.
FIG. 3 shows a schematic block diagram of a drive circuit of a probe of one embodiment of the present application.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The utility model comprises a sealed cabin shell, an optical window on the shell, a Light Emitting Diode (LED) light source arranged in the shell, a band-pass filter, a photoelectric detector and a drive circuit board; an LED light source and a photoelectric detector (detect) are correspondingly arranged on the inner side of the optical window, and an optical filter is arranged at the front end of the photoelectric detector; the LED light source and the center of the detector are fixed at a proper angle; the LED light source and the photoelectric detector are connected to the driving circuit board; the back end cover of the shell is provided with a watertight plug.
Optical probe, the submergence is at aquatic work, constant current source drive modulation circuit control LED light source on the dirver circuit board sends high frequency modulation signal, the transmission light that has certain divergence angle sees through the glass window and shines the aquatic, the backscattering signal and the chlorophyll fluorescence signal of its production see through the glass window, reachs photoelectric detector after band pass filter filters, photoelectric detector accomplishes light signal to the signal of telecommunication conversion, dirver circuit board enlargies the signal of telecommunication, filtering, demodulation processing. The optical probe is powered and communicates with the upper computer through a watertight plug on the housing. In the using process, the optical probe can be used for in-situ online monitoring in water after a configuration file is imported into control software by calibrating on site in advance.
The glass window can be made of quartz glass or sapphire materials.
The central wavelength of the LED light source is 450nm, 550nm and 680nm, respectively, and it should be understood that the central wavelength of the LED light source may also be other wavelengths, which is not limited in this application.
The center wavelengths of the band-pass filters are respectively 450nm, 550nm and 680nm, and the full width at half maximum is 20-40 nm.
The photoelectric detector can select a PN photodiode with small dark current, and has good spectral response in a wide spectral range of 190nm-1000 nm.
The driving circuit board comprises a signal processing circuit of an LED light source modulation and a photoelectric detector. The LED light source adopts a working mode of time-sharing emission and photoelectric detector multiplexing, firstly, the LEDs with central wavelengths of 550nm and 680nm emit light simultaneously, and the photoelectric detectors corresponding to the band-pass filters of 550nm and 680nm receive back scattering signals of the LEDs simultaneously; then, the LED with the central wavelength of 450nm emits light, and backscattering signals and chlorophyll fluorescence signals of the LED are received by the bandpass filters corresponding to 450nm and 680 nm. The drive circuit amplifies and filters signals collected by the photoelectric detector, and outputs information to the upper computer through the watertight plug. The shell adopts a sealing structure, has a waterproof function, adopts an aluminum alloy material, and carries out hard oxidation treatment.
The utility model discloses a light source and photoelectric detector are shown in figure 1 at probe front end housing overall arrangement, have presented 6 optical window in the picture, these six optical window all with probe front end housing face parallel placement, the optical window material is quartz glass or sapphire. The specific structure of the probe is shown in fig. 2. Measuring the backscattering signal of the 550nm emitted light in the 117-degree direction by adopting a 550nm LED light source and a photoelectric detector 1 (figure 2 (a)); a 680nm LED light source is adopted to be matched with the photoelectric detector 2 to measure a backscattering signal of 680nm emitted light in a 117-degree direction, and a 450nm LED light source is matched with the photoelectric detector 2 to measure a chlorophyll fluorescence signal at 680nm, wherein a photoelectric detector multiplexing working mode (figure 2(b)) is adopted; the backscattered signal of the 450nm emitted light in the 142 ° direction is measured using a 450nm LED light source in conjunction with photodetector 3, where the LED light source is shared with fluorescence detection (fig. 2 (c)). The specific time-sharing working mode is as follows: the LEDs with central wavelengths of 550nm and 680nm emit light simultaneously, and the photoelectric detectors corresponding to the band-pass filters of 550nm and 680nm receive back scattering signals of the LEDs simultaneously; then, the 450nm central wavelength LED emits light, and backscattering signals and chlorophyll fluorescence signals of the LED are received by corresponding 450nm band-pass filters and 680nm band-pass filters.
The driving circuit of the present invention is shown in fig. 3, and mainly comprises: the LED constant current source driving circuit comprises an LED light source, a constant current source driving modulation circuit, a low-power consumption single chip microcomputer, an A/D converter, a synchronous demodulator, a secondary amplifier, a band-pass filter, a preamplifier, an I/V converter, a photoelectric detector, an RS232 communication unit and a PC terminal. The method is characterized in that: an independent optical power adjusting circuit is arranged for the LED light source, so that interference-free independent adjustment is realized; the PWM adjustable multi-path output function is adopted to realize the fine phase adjustment of the chopped wave signals; crosstalk between signals is reduced by enhancing isolation of the analog circuitry from the digital circuitry. In the actual use process, the optical probe can be used for in-situ online monitoring in water after a configuration file is imported into control software by calibrating on site in advance.
Those skilled in the art will clearly understand that the techniques in the embodiments of the present application may be implemented by way of software plus a required general hardware platform. Based on such understanding, the technical solutions in the embodiments of the present application may be essentially implemented or a part contributing to the prior art may be embodied in the form of a software product, which may be stored in a storage medium, such as a ROM/RAM, a magnetic disk, an optical disk, etc., and includes several instructions for enabling a computer device (which may be a personal computer, a server, or a network device, etc.) to execute the method described in the embodiments or some parts of the embodiments of the present application.
It is noted that, in this document, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The foregoing are merely exemplary embodiments of the present invention, which enable those skilled in the art to understand or practice the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
The same and similar parts in the various embodiments in this specification may be referred to each other. Especially, for the terminal embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant points can be referred to the description in the method embodiment.
The above-described embodiments of the present application do not limit the scope of the present application.
Claims (5)
1. An underwater multi-wavelength backscatter and fluorescence monitoring probe, comprising: the device comprises a sealed cabin shell, a first excitation light source (1), a second excitation light source (2), a third excitation light source (3), a first photoelectric detector (4), a second photoelectric detector (5), a third photoelectric detector (6) and a constant current source driving modulation circuit, wherein the first excitation light source, the second excitation light source, the third excitation light source, the first photoelectric detector, the second photoelectric detector and the third photoelectric detector are positioned in the sealed cabin shell;
the central wavelengths of the first excitation light source (1), the second excitation light source (2) and the third excitation light source (3) are different;
the probe further comprises: the first band-pass filter is positioned at the front end of the first photoelectric detector (4), the second band-pass filter is positioned at the front end of the second photoelectric detector (5), and the third band-pass filter is positioned at the front end of the third photoelectric detector (6), wherein the band-pass center wavelength of the first band-pass filter is the same as the center wavelength of the first excitation light source (1), and the band-pass center wavelength of the second band-pass filter is the same as the center wavelength of the second excitation light source (2); the bandpass center wavelength of the third bandpass filter is the same as the center wavelength of the third excitation light source (3);
the first photoelectric detector (4) is used for detecting a backscattering signal of the first excitation light source (1) at 117 degrees;
the second photoelectric detector (5) is used for detecting a backscattering signal of the second excitation light source (2) at 117 degrees, and the second photoelectric detector (5) is also used for detecting a chlorophyll fluorescence signal of the third excitation light source (3) at 142 degrees;
the third photoelectric detector (6) is used for detecting a backscattering signal of the third excitation light source (3) at 142 degrees;
the constant current source driving modulation circuit is used for controlling only the first excitation light source (1) and the second excitation light source (2) to emit light in a first time period, and is also used for controlling only the third excitation light source (3) to emit light in a second time period, and no overlapping part exists between the first time period and the second time period.
2. The probe according to claim 1, wherein the first excitation light source (1) is a light emitting diode with a central wavelength of 550nm, the second excitation light source (2) is a light emitting diode with a central wavelength of 680nm, the third excitation light source (3) is a light emitting diode with a central wavelength of 450nm, the first bandpass filter has a central wavelength of 550nm, the second bandpass filter has a central wavelength of 680nm, and the third bandpass filter has a central wavelength of 450 nm.
3. The probe according to claim 2, wherein the constant current source drive modulation circuit high-frequency modulates the first excitation light source (1), the second excitation light source (2), and the third excitation light source (3) with a constant current source drive circuit.
4. The probe of claim 3, further comprising: a first optical window, a second optical window, a third optical window, a fourth optical window, a fifth optical window, and a sixth optical window, the first optical window is placed in front of the first excitation light source (1), the second optical window is placed in front of the second excitation light source (2), the third optical window is placed in front of the third excitation light source (3), the fourth optical window is placed in front of the first bandpass filter, the fifth optical window is placed before the second bandpass filter, the sixth optical window is placed before the third bandpass filter, the first optical window, the second optical window, the third optical window, the fourth optical window, the fifth optical window and the sixth optical window are all arranged in parallel with the front end cover face of the probe, and the optical windows are made of quartz glass or sapphire.
5. The probe of claim 4, further comprising: and the watertight plug is positioned on the sealed cabin shell.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116930141A (en) * | 2023-09-13 | 2023-10-24 | 国家海洋技术中心 | CDOM deep sea sensor by ultraviolet fluorescence analysis method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116930141A (en) * | 2023-09-13 | 2023-10-24 | 国家海洋技术中心 | CDOM deep sea sensor by ultraviolet fluorescence analysis method |
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