CN105628657A - Chlorophyll fluorescence detector device - Google Patents
Chlorophyll fluorescence detector device Download PDFInfo
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- CN105628657A CN105628657A CN201410600200.0A CN201410600200A CN105628657A CN 105628657 A CN105628657 A CN 105628657A CN 201410600200 A CN201410600200 A CN 201410600200A CN 105628657 A CN105628657 A CN 105628657A
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- fluorescence detector
- chlorophyll fluorescence
- signal processing
- module
- processing module
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Abstract
The invention belongs to the field of environmental instrument, and more specifically relates to a chlorophyll fluorescence detector device. The chlorophyll fluorescence detector device comprises a working power supply, an optical module, a signal processing module, and a data communication module; the optical-electric module is used for realizing excitation light source modulation, amplification of weak carrier signals, and current/voltage conversion at the same time; the signal processing module is used for realizing amplification and denoising of received signals, and examining wave measuring results; and the data acquisition module is used for storage disposal of collected signals via connection of RS232 with an upper computer. The chlorophyll fluorescence detector device can be used for water eutrophication on-site detecting and monitoring of lakes, rivers, and the sea, and is high in sensitivity, speed, and instantaneity, and no reagent is used.
Description
Technical field
The present invention relates to environmental instrument field, be specifically related to the instrument of a kind of chlorophyll fluorescence detection.
Background technology
In recent years, there are the water crises such as Taihu Lake, Wuhu, Song Hua River year after year in China, owing to blue green algae continues to break out in water body so that in water phytoplankton increase severely, water oxygen divide exhaust, water body presents large area anaerobic condition, organism in water is mortality therefore. Due to the fast-growth of algae in water, on the water surface, chlorella is piled up, and causes current difficulty, and water body cannot carry out air with the external world and exchange, and becomes stench day by day, all brings great harm to the production of people, life and life and health.
The Eutrophic Extent of water body has mostly been monitored by relevant monitoring station and the Environmental Research Institute of current domestic each lake region reservoir, and including COD, total nitrogen, total phosphorus, Chlorophyll-a Content etc., single index and the Evaluation of Eutrophication of aggregative index calculated by certain model draw. Aggregative index evaluation mostly carries out cell cultivation under laboratory or specified conditions and carries out, and due to the problem such as water sampling and transport, there is bigger survey and starts error, and be not on-site measurement, and its result has very big time-lag effect, and repeatability is difficult to ensure that.
Summary of the invention
In order to solve the problems referred to above, the invention provides a kind of chlorophyll fluorescence detecting instrument.
The present invention is achieved through the following technical solutions:
Chlorophyll fluorescence detector, including working power, optical module, signal processing module and data communication module, it is characterized in that: described optical module includes SCM PWM, LED and photodiode, and described signal processing module includes preamplifier, differential amplifier; Described data communication module includes embedded integration A/D and host computer; It is connected by photodiode between described optical module and signal processing module, is connected by embedded integration A/D between signal processing module with data communication module.
It is connected by analog switch between above-mentioned working power, SCM PWM and LED, between described LED and photodiode, is connected to flow cell.
Above-mentioned photodiode is provided with transreactance amplifier.
Above-mentioned signal processing module preamplifier outfan passes through single-chip microcomputer PGA programming amplifying, is carrying out differential amplification.
Above-mentioned data communication module adopts embedded integration A/D to be connected with host computer by RS232.
The technique effect that the present invention is useful:
Chlorophyll fluorescence detector of the present invention, including working power, optical module, signal processing module and data communication module, described optical-electric module realizes excitation source modulation, faint carrier signal is amplified and realizes current/voltage-converted simultaneously, the signal received is amplified by signal processing module, the measurement result of the ripple that goes out to make an uproar, checks, and the signal collected is connected with host computer by RS232 signal is carried out storage process by data acquisition module. The present invention realizes can be used for the technology of the water eutrophication Site Detection monitoring such as lake, river, ocean, has highly sensitive ' without reagent, speed is fast, real-time is good advantage.
Accompanying drawing explanation
The flow chart of Fig. 1 chlorophyll fluorescence detector of the present invention.
Detailed description of the invention
Chlorophyll fluorescence detector, including working power, optical module, signal processing module and data communication module, it is characterized in that: described optical module includes SCM PWM, LED and photodiode, and described signal processing module includes preamplifier, differential amplifier; Described data communication module includes embedded integration A/D and host computer; It is connected by photodiode between described optical module and signal processing module, is connected by embedded integration A/D between signal processing module with data communication module. It is connected by analog switch between described working power, SCM PWM and LED, between described LED and photodiode, is connected to flow cell.
Specifically, chlorophyll fluorescence detector, mainly become with data communication module set by optical module, signal processing module. In optical module, excitation source adopts peak wavelength to be the blue led of 460mm, SCM PWM export l024Hz square wave as carrier signal, constant-current source is become by analog switch the current impulse of high frequency, power to LED with this current impulse, it is achieved excitation source is modulated. Also very faint for the weak fluorescence signals a lot, that excite of the power light source light intensity such as the beam intensity ratio laser instrument of LED, mercury lamp, xenon lamp, fluoroscopic examination module has been selected and has been specifically designed for the transreactance amplifier that photodiode is arranged, and realizes current/voltage-converted while small-signal being amplified.
Signal processing module is mainly signal gain and adjusts circuit. First preposition amplification is carried out from the voltage signal of photodiode output; The signal of the weak output of prime enters the integrated PGA of single-chip microcomputer and carries out programming amplifying; For eliminating the noise that bias light produces, adopt differential amplification of AD620 to remove background noise impact, after detection, after, obtain the measurement result needed.
Data communication module adopts the integrated A/D of system embedment formula to realize signals collecting, coding by RS232 and host computer or data collecting system communication in PSoCDesigner.
Chlorophyll fluorescence detector of the present invention, including working power, optical module, signal processing module and data communication module, described optical-electric module realizes excitation source modulation, faint carrier signal is amplified and realizes current/voltage-converted simultaneously, the signal received is amplified by signal processing module, the measurement result of the ripple that goes out to make an uproar, checks, and the signal collected is connected with host computer by RS232 signal is carried out storage process by data acquisition module. The present invention realizes can be used for the technology of the water eutrophication Site Detection monitoring such as lake, river, ocean, has highly sensitive ' without reagent, speed is fast, real-time is good advantage.
Claims (5)
1. chlorophyll fluorescence detector, including working power, optical module, signal processing module and data communication module, it is characterized in that: described optical module includes SCM PWM, LED and photodiode, and described signal processing module includes preamplifier, differential amplifier; Described data communication module includes embedded integration A/D and host computer; It is connected by photodiode between described optical module and signal processing module, is connected by embedded integration A/D between signal processing module with data communication module.
2. chlorophyll fluorescence detector as claimed in claim 1, it is characterised in that: it is connected by analog switch between described working power, SCM PWM and LED, between described LED and photodiode, is connected to flow cell.
3. chlorophyll fluorescence detector as described in claims 1 or 2, it is characterised in that: described photodiode is provided with transreactance amplifier.
4. chlorophyll fluorescence detector as claimed in claim 1, it is characterised in that: described signal processing module preamplifier outfan passes through single-chip microcomputer PGA programming amplifying, is carrying out differential amplification.
5. chlorophyll fluorescence detector as claimed in claim 1, it is characterised in that: described data communication module adopts embedded integration A/D to be connected with host computer by RS232.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410600200.0A CN105628657A (en) | 2014-10-31 | 2014-10-31 | Chlorophyll fluorescence detector device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410600200.0A CN105628657A (en) | 2014-10-31 | 2014-10-31 | Chlorophyll fluorescence detector device |
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| CN105628657A true CN105628657A (en) | 2016-06-01 |
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| CN201410600200.0A Pending CN105628657A (en) | 2014-10-31 | 2014-10-31 | Chlorophyll fluorescence detector device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994005A (en) * | 2016-05-30 | 2016-10-12 | 江苏师范大学 | Detection instrument and method for cow oestrus degree and optimum insemination time |
| CN106896090A (en) * | 2017-04-25 | 2017-06-27 | 清华大学深圳研究生院 | A kind of Fresh Watcr Blue Algae biology amount detection systems and its detection method |
| EP3943918A1 (en) | 2020-07-22 | 2022-01-26 | Universidade do Minho | Device for identification and quantification of phytoplankton, methods and uses thereof |
-
2014
- 2014-10-31 CN CN201410600200.0A patent/CN105628657A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994005A (en) * | 2016-05-30 | 2016-10-12 | 江苏师范大学 | Detection instrument and method for cow oestrus degree and optimum insemination time |
| CN105994005B (en) * | 2016-05-30 | 2022-04-19 | 江苏师范大学 | Instrument and method for detecting oestrus degree and optimal insemination time of dairy cow |
| CN106896090A (en) * | 2017-04-25 | 2017-06-27 | 清华大学深圳研究生院 | A kind of Fresh Watcr Blue Algae biology amount detection systems and its detection method |
| EP3943918A1 (en) | 2020-07-22 | 2022-01-26 | Universidade do Minho | Device for identification and quantification of phytoplankton, methods and uses thereof |
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Application publication date: 20160601 |
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| WD01 | Invention patent application deemed withdrawn after publication |