CN213835360U - Cell culture device relating to research and development of rabies vaccine - Google Patents
Cell culture device relating to research and development of rabies vaccine Download PDFInfo
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- CN213835360U CN213835360U CN202021928291.8U CN202021928291U CN213835360U CN 213835360 U CN213835360 U CN 213835360U CN 202021928291 U CN202021928291 U CN 202021928291U CN 213835360 U CN213835360 U CN 213835360U
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Abstract
The utility model relates to the technical field of cell culture devices, in particular to a cell culture device relating to research and development of rabies vaccines, which comprises an outer bottle of the device, a culture bottle, a heating device, an outer cover mechanism, hasps, an inlet pipe and an air pump, wherein the culture bottle is arranged in the outer bottle of the device, the heating device is arranged on one side of the outer bottle of the device, the upper end of the outer bottle of the device is fixed with the outer cover mechanism through a plurality of hasps, the inlet pipe is arranged on the outer cover mechanism, the air blowing mechanism is arranged in the culture bottle, and the air blowing mechanism is connected with the air pump; the air pressure valve has the functions of filtering and regulating the pressure of air, and the pressure of the entering air can be regulated through the air pressure valve according to the concentration of cells in the culture bottle in the using process, so that the gathered cell clusters can be blown away more effectively.
Description
Technical Field
The utility model relates to a cell culture device technical field specifically is a cell culture device that relates to rabies bacterin research and development.
Background
The cell culture technology is also called as cell cloning technology, is the most core and basic technology in biotechnology, most biological products are generally obtained by cell culture, and the cell culture is widely applied to various fields of biology, medicine, new drug research and development and the like, and becomes one of the most important basic sciences. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques. The cell culture technology can be used for preparing simple single cells or rarely differentiated multi-cells from one cell through mass culture, and is a large-scale cell cloning technology.
In the process of culturing cells, the temperature of the culture flask or the culture dish needs to be controlled according to different conditions, and the cells need to be blown to eliminate the aggregation of cell masses. Among the prior art, generally need the experimenter operation to blow to beat injection, straw or centrifuging tube etc. and press to the cell and blow and beat, both spent time and energy to manual operation's accuracy is poor, and is poor to the control of pressure in the operation process, blows and beats the activity that the in-process is inhomogeneous, too big or the undersize to the pressure of cell and all can influence the cell, thereby influences final culture result, for this reason, the utility model provides a cell culture device that relates to rabies vaccine research and development is used for solving above-mentioned problem.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell culture device that relates to rabies vaccine research and development to solve the problem of proposing among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a cell culture device related to research and development of rabies vaccines comprises an outer bottle, a culture bottle, a heating device, an outer cover mechanism, hasps, a feeding pipe and an air pump, wherein the culture bottle is arranged in the outer bottle, the heating device is arranged on one side of the outer bottle, the upper end of the outer bottle is fixed with the outer cover mechanism through the hasps, the feeding pipe is arranged on the outer cover mechanism, an air blowing mechanism is arranged in the culture bottle, and the air blowing mechanism is connected with the air pump;
the air blowing mechanism comprises an air inlet pipe, one end of the air inlet pipe penetrates through the outer cover mechanism until the inside of the culture bottle and is fixedly connected with the blowing and beating needle, the other end of the air inlet pipe is fixedly connected with the two inner rings of the bearing, the two outer rings of the bearing are fixedly connected with the joint, one end of the telescopic pipe is fixedly connected with the joint, the other end of the telescopic pipe is fixedly connected with the air pressure valve, and the air pressure valve is connected with the air pump through an air pipe.
Preferably, the heating device comprises a temperature control box fixedly connected with the outer wall of the outer bottle of the device, the outer wall of the outer bottle of the device is provided with a plurality of heating boxes, and the bottom in the outer bottle of the device is symmetrically provided with two groups of temperature sensing probes along the central line.
Preferably, the outer cover mechanism comprises an outer cover mechanism shell, the outer cover mechanism shell is fixed with the outer bottle of the device through a plurality of hasps, a first bearing is arranged right above the culture bottle and fixedly connected with the inner wall of the outer cover mechanism shell, an inner ring of the first bearing is fixedly connected with a transmission plate, the upper end of the transmission plate is fixedly connected with a transmission shaft, and the transmission shaft is connected with a power mechanism.
Preferably, the heating box includes the heating box shell, and the outer bottle outer wall of heating box shell embedding device sets up the resistance wire in the heating box shell, and resistance wire one side sets up the asbestos tile, and asbestos tile fixed connection heating box shell.
Preferably, the power mechanism comprises a large gear, the large gear is fixed with the transmission shaft through a flat key, the large gear is meshed with a small gear, the small gear is fixedly connected with a motor, and the motor is fixedly connected with the inner wall of the shell of the outer cover mechanism.
Preferably, two groups of through holes are formed in the gear wheel, the feeding pipe penetrates through one group of through holes in the gear wheel until the inside of the culture bottle, and the air inlet pipe penetrates through the other group of through holes in the gear wheel until the inside of the culture bottle.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the air pressure valve has the functions of filtering and regulating air pressure, and the pressure of the entering gas can be regulated through the air pressure valve according to the concentration of cells in the culture bottle in the using process, so that the gathered cell clusters can be blown away more effectively;
2. eight groups of equal angles are arranged on the same circumferential line, eight groups of equal angles are arranged on the inner wall of the heating box shell, and one group of equal angles are arranged at the bottom of the heating box shell, so that cells in the culture bottle are heated more uniformly.
Drawings
FIG. 1 is a front view of the structure of the present invention;
FIG. 2 is an enlarged view of FIG. 1A;
FIG. 3 is a sectional view of the cover mechanism of the present invention;
fig. 4 is a combined view of the first structural bearing, the transmission plate, the transmission shaft and the gearwheel of the present invention.
In the figure: the device comprises 1 device of an external bottle, 2 culture bottles, 3 heating devices, 4 external cover mechanisms, 5 hasps, 6 blowing mechanisms, 7 feeding pipes, 8 air pumps, 301 temperature control boxes, 302 heating boxes, 303 temperature sensing probes, 3021 resistance wires, 3022 heating box shells, 3023 asbestos tiles, 401 external cover mechanism shells, 402 bearing I, 403 transmission plates, 404 transmission shafts, 405 power mechanisms, 4051 pinions, 4052 gearwheels, 4053 motors, 601 blowing needles, 602 air inlet pipes, 603 bearing II, 604 connectors, 605 extension pipes and 606 air pressure valves.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1 to 4, the present invention provides a technical solution: a cell culture device relating to the research and development of rabies vaccines comprises an outer bottle 1 of the device, a culture bottle 2, a heating device 3, an outer cover mechanism 4, hasps 5, a feeding pipe 7 and an air pump 8, wherein the culture bottle 2 is arranged in the outer bottle 1 of the device, one side of the outer bottle 1 of the device is provided with the heating device 3, the upper end of the outer bottle 1 of the device is fixed with the outer cover mechanism 4 through a plurality of hasps 5, the feeding pipe 7 is arranged on the outer cover mechanism 4, an air blowing mechanism 6 is arranged in the culture bottle 2, the air blowing mechanism 6 is connected with the air pump 8 and comprises an air inlet pipe 602, one end of the air inlet pipe 602 penetrates through the outer cover mechanism 4 until the inside of the culture bottle 2 is fixedly connected with a blow-beating needle 601, the other end of the air inlet pipe 602 is fixedly connected with the inner ring of a bearing II 603, the outer ring of the bearing II 603 is fixedly connected with a joint 604, one end of a telescopic pipe 605 is fixedly connected with one end of the telescopic pipe 605, and the other end of the air pressure valve 606 (refer to model AW2-02), the air pressure valve 606 (reference model AW2-02) is connected with the air pump 8 through an air pipe, wherein the air pressure valve 606 (reference model AW2-02) has the functions of filtering and regulating pressure of air, in the using process, the pressure of the entering air can be regulated through the air pressure valve 606 (reference model AW2-02) according to the concentration of cells in the culture bottle 2, so that the gathered cell clusters can be blown away more effectively, the telescopic length of the telescopic pipe 605 meets the stroke of the air inlet pipe 602 during rotation, the second bearing 603 is set to prevent the telescopic pipe 605 from being wound on the air inlet pipe 602, and after the air pressure value of the air pressure valve 606 (reference model AW2-02) is set, air can enter the telescopic pipe 605 from the air pressure valve 606 (reference model AW2-02), the telescopic pipe 605 enters the air inlet pipe 602 through the connector 604 and is finally sprayed out from the head of the blowing needle 601.
Further, the heating device 3 comprises a temperature control box 301, the temperature control box 301 is fixedly connected with the outer wall of the device outer bottle 1, a plurality of heating boxes 302 are arranged on the outer wall of the device outer bottle 1, two groups of temperature sensing probes 303 are symmetrically arranged along the central line at the bottom in the device outer bottle 1, the heating boxes 302 comprise heating box shells 3022, the heating box shells 3022 are embedded in the outer wall of the device outer bottle 1, resistance wires 3021 are arranged in the heating box shells 3022, asbestos tiles 3023 are arranged on one side of the resistance wires 3021, the asbestos tiles 3023 are fixedly connected with the heating box shells 3022, wherein the resistance wires 3021 and the temperature sensing probes 303 are both in line connection with the temperature control box 301, the heating boxes 302 are provided with nine groups, eight groups are arranged on the same circumferential line, eight groups are arranged on the outer wall of the device outer bottle 1, one group is arranged at the bottom of the device outer bottle 1, so that the cells in the culture bottle 2 are heated more uniformly, when the resistance wires 3021 are electrified, the heat dissipation begins, when the temperature reaches the ideal temperature, the temperature sensing probe 303 transmits a signal to the temperature control box 301, so that the heat dissipation of the resistance wire 3021 is controlled, and the culture bottle 2 is kept in an ideal constant temperature state.
Further, the outer cover mechanism 4 comprises an outer cover mechanism shell 401, the outer cover mechanism shell 401 is fixed with the outer bottle 1 through a plurality of hasps 5, a bearing I402 is arranged right above the culture bottle 2, the bearing I402 is fixedly connected with the inner wall of the outer cover mechanism shell 401, the inner ring of the bearing I402 is fixedly connected with a transmission plate 403, the upper end of the transmission plate 403 is fixedly connected with a transmission shaft 404, the transmission shaft 404 is connected with a power mechanism 405, the power mechanism 405 comprises a large gear 4052, the large gear 4052 is fixed with the transmission shaft 404 through a flat key, the large gear 4052 is meshed with a small gear 4051, the small gear 4051 is fixedly connected with a motor 4053, the motor 4053 is fixedly connected with the inner wall of the outer cover mechanism shell 401, wherein two groups of through holes are arranged on the large gear 4052, a feed pipe 7 passes through one group of through holes on the large gear 4052 to the inside the culture bottle 2, an air inlet pipe 602 passes through the other group of through holes on the large gear 4052 to the inside the culture bottle 2, wherein when the motor 4053 is started to drive the small gear 4051 to rotate, meanwhile, the large gear 4052 rotates together, and the large gear 4052 rotates the transmission plate 403 through the transmission shaft 404, so as to drive the air inlet pipe 602 to rotate, so that the cell mass of the culture bottle 2 can be uniformly blown by the blowing needle 601.
The working principle is as follows: in the use, with during cell and required nourishment pass through the inlet pipe leading-in blake bottle 2, resistance wire 3021 circular telegram begins the heat dissipation, when the temperature reaches the ideal, temperature sensing probe 303 gives temperature control box 301 with signal transmission, thereby the heat dissipation of control resistance wire 3021, let blake bottle 2 keep the constant temperature state of ideal, motor 4053 starts to drive pinion 4051 rotatory, gear wheel 4025 rotates together simultaneously, gear wheel 4025 makes driving plate 403 rotatory through transmission shaft 404, thereby it is rotatory to drive intake pipe 602, the cell mass that makes blake bottle 2 can both be even receives the blowing of blowing needle 601.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a cell culture device that relates to rabies vaccine research and development, includes device outer bottle (1), blake bottle (2), heating device (3), enclosing cover mechanism (4), hasp (5), inlet pipe (7), air pump (8), its characterized in that: a culture bottle (2) is arranged in the device outer bottle (1), a heating device (3) is arranged on one side of the device outer bottle (1), the upper end of the device outer bottle (1) is fixed with an outer cover mechanism (4) through a plurality of hasps (5), a feeding pipe (7) is arranged on the outer cover mechanism (4), a blowing mechanism (6) is arranged in the culture bottle (2), and the blowing mechanism (6) is connected with an air pump (8);
the air blowing mechanism (6) comprises an air inlet pipe (602), one end of the air inlet pipe (602) penetrates through the outer cover mechanism (4) until the inside of the culture bottle (2) is fixedly connected with the blowing needle (601), the other end of the air inlet pipe (602) is fixedly connected with the inner ring of a second bearing (603), the outer ring of the second bearing (603) is fixedly connected with a joint (604), the joint (604) is fixedly connected with one end of a telescopic pipe (605), the other end of the telescopic pipe (605) is fixedly connected with an air pressure valve (606), and the air pressure valve (606) is connected with the air pump (8) through an air pipe.
2. The cell culture apparatus relating to rabies vaccine development according to claim 1, wherein: heating device (3) include temperature control case (301), outer bottle (1) outer wall of temperature control case (301) fixed connection device, the outer bottle (1) outer wall of device sets up a plurality of heating boxes (302), and the bottom sets up two sets of temperature sensing probe (303) along the central line symmetry in the outer bottle (1) of device.
3. The cell culture apparatus relating to rabies vaccine development according to claim 1, wherein: enclosing cover mechanism (4) are including enclosing cover mechanism casing (401), enclosing cover mechanism casing (401) are fixed with device outer bottle (1) through a plurality of hasps (5), set up bearing (402) directly over blake bottle (2), bearing (402) fixed connection enclosing cover mechanism casing (401) inner wall, bearing (402) inner circle fixed connection driving plate (403), driving plate (403) upper end fixed connection transmission shaft (404), power unit (405) is connected in transmission shaft (404).
4. The cell culture apparatus relating to rabies vaccine development according to claim 2, wherein: heating box (302) is including heating box shell (3022), outer bottle (1) outer wall of heating box shell (3022) embedded device sets up resistance wire (3021) in heating box shell (3022), resistance wire (3021) one side sets up asbestos tile (3023), asbestos tile (3023) fixed connection heating box shell (3022).
5. The cell culture apparatus relating to rabies vaccine development according to claim 3, wherein: the power mechanism (405) comprises a large gear (4052), the large gear (4052) is fixed with the transmission shaft (404) through a flat key, the large gear (4052) is meshed with a small gear (4051), the small gear (4051) is fixedly connected with a motor (4053), and the motor (4053) is fixedly connected with the inner wall of the outer cover mechanism shell (401).
6. The cell culture apparatus relating to rabies vaccine development according to claim 5, wherein: set up two sets of through-holes on gear wheel (4052), inlet pipe (7) pass a set of through-hole on gear wheel (4052) and reach culture bottle (2) inside, and intake pipe (602) pass another set of through-hole on gear wheel (4052) and reach culture bottle (2) inside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021928291.8U CN213835360U (en) | 2020-09-07 | 2020-09-07 | Cell culture device relating to research and development of rabies vaccine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021928291.8U CN213835360U (en) | 2020-09-07 | 2020-09-07 | Cell culture device relating to research and development of rabies vaccine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213835360U true CN213835360U (en) | 2021-07-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021928291.8U Active CN213835360U (en) | 2020-09-07 | 2020-09-07 | Cell culture device relating to research and development of rabies vaccine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213835360U (en) |
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2020
- 2020-09-07 CN CN202021928291.8U patent/CN213835360U/en active Active
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Address after: No.32, Xinglin Road, medical hi tech Zone, Taizhou City, Jiangsu Province 225312 Patentee after: Jiangsu Zhonghui Yuantong Biotechnology Co.,Ltd. Address before: No.32, Xinglin Road, medical hi tech Zone, Taizhou City, Jiangsu Province 225312 Patentee before: AB&B BIO TECH CO.,LTD. JS |