CN213475975U - Novel strain liquid counting culture bottle - Google Patents
Novel strain liquid counting culture bottle Download PDFInfo
- Publication number
- CN213475975U CN213475975U CN202021592293.4U CN202021592293U CN213475975U CN 213475975 U CN213475975 U CN 213475975U CN 202021592293 U CN202021592293 U CN 202021592293U CN 213475975 U CN213475975 U CN 213475975U
- Authority
- CN
- China
- Prior art keywords
- bottle
- bottle body
- cap
- counting culture
- liquid counting
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 239000007788 liquid Substances 0.000 title claims abstract description 28
- 239000004033 plastic Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 241001148470 aerobic bacillus Species 0.000 description 2
- 230000003670 easy-to-clean Effects 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 2
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a novel bacterial liquid count blake bottle. The culture bottle comprises a bottle body with a smooth surface, wherein the length of the bottle body is 8cm, the width of the bottle body is 5cm, the height of the bottle body is 0.5cm, a protruding bottle opening is formed in the side face of the bottle body, a cylindrical bottle cap is installed on the bottle opening, and the bottle opening extends towards the upper side in an inclined mode. The novel strain liquid counting culture bottle provided by the utility model cultures and visually observes strains by arranging the bottle body with the cuboid container and the cylindrical bottle cap, can accurately and effectively count, avoids the condition of missing or multiple counts as much as possible, and ensures the accuracy of counting; the structure is simple, the manufacture and the use are convenient, the cost is low, and the practicability is high.
Description
Technical Field
The utility model relates to a biological medicine experimental facilities field, in particular to novel bacterial liquid count blake bottle.
Background
In biomedical experiments, the step of liquid culture of strains is often included, and during the culture process, the strains are often counted. The common mode of liquid culture strains is to place the strains in a test tube filled with liquid for culture and counting, the test tube is a common cylindrical glass tube, a cover or other sealing devices are additionally arranged on an upper end opening, and the bottom of the test tube is of a semicircular closed structure. When the number of the cultured strains in the test tube is counted, the strains are overlapped to form concave-convex parts, so that the conditions of sight line observation deviation and inaccurate reading are easily caused; and because the planes of all parts of the strains are different, the test tube needs to be rotated ceaselessly during counting, and then suspended strains cultured in the test tube move, so that the condition of forgetting or remembering more strains is caused, and the counting accuracy is seriously influenced. In addition, the sealing device such as a cap attached to the opening of the test tube may obstruct the circulation of air, and may cause some strains, such as aerobic strains, to be produced in a poor condition.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems, the utility model provides a novel strain liquid counting culture bottle.
According to an aspect of the utility model, a novel bacterial liquid count blake bottle is provided, including a bottle that the surface is smooth, the length of bottle is 8cm, and the width is 5cm, highly is 0.5cm, the side of bottle is equipped with a prominent bottleneck, install a columniform bottle lid on the bottleneck, wherein, the bottleneck extends towards the top slope.
The novel strain liquid counting culture bottle provided by the utility model cultures and visually observes strains by arranging the bottle body with the cuboid container and the cylindrical bottle cap, can accurately and effectively count, avoids the condition of missing or multiple counts as much as possible, and ensures the accuracy of counting; the structure is simple, the manufacture and the use are convenient, the cost is low, and the practicability is high.
In some embodiments, the length and width of the body are enlarged according to a scale and the height is enlarged by no more than the scale. Therefore, in practical use, the bottle body can be enlarged or reduced in a proper equal ratio, but the height of the bottle body still needs to be as small as possible.
In some embodiments, the vial is made of plastic. Thus, the preferred material for the body is a plastic, such as a polymeric synthetic plastic.
In some embodiments, the vial is made of glass or rubber. Thus, other suitable materials may be used without the choice of plastic.
In some embodiments, the vial is a disposable sterile vial. Therefore, the bottle body is small in height, so that the whole bottle is not easy to clean, and the bottle body can be set into a disposable aseptic bottle.
In some embodiments, the bottle cap is a hermetic cap. Therefore, when the anaerobic bacteria are cultured and counted, the closed cover is selected and the bottle body is filled with the liquid as much as possible so as to reduce the oxygen amount in the bottle body.
In some embodiments, the bottle cap is an air filter cap. Therefore, when the aerobic bacteria are cultured and counted, the air filter element cover can be selected to ensure that clean oxygen can smoothly enter the bottle body.
In some embodiments, the outer periphery of the mouth and the inner periphery of the cap are provided with corresponding threads, respectively. Therefore, the bottle mouth and the bottle cap can be stably connected and sealed through the threads.
In some embodiments, the side of the body has volume graduations. From this, can read the total volume of liquid in the bottle through the volume scale mark.
In some embodiments, the bottom surface or the top surface of the bottle body is provided with a grid line, so that the bottle body on the body is divided into a plurality of grids with similar areas. Therefore, for the situation that the amount of the strains is large, dividing lines can be drawn on the bottle body for dividing, when counting is carried out, the bottle body is inverted and uniformly mixed, the number of each strain in one or more than one division is counted, and the total amount of the strains is estimated according to the volume in the bottle corresponding to the division.
Drawings
FIG. 1 is a schematic structural view of a novel strain liquid counting culture flask according to an embodiment of the present invention;
FIG. 2 is a top view of the novel seed liquid count flask of FIG. 1;
FIG. 3 is a side view of the novel seed liquid count flask of FIG. 1.
In the figure: the bottle comprises a bottle body 1, a bottle opening 2, a bottle cap 3, volume scale lines 4 and division lines 5.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Fig. 1 schematically shows a structure of a novel strain liquid counting culture flask according to an embodiment of the present invention, fig. 2 shows a top view of the novel strain liquid counting culture flask of fig. 1, and fig. 3 shows a side view of the novel strain liquid counting culture flask of fig. 1. As shown in fig. 1-3, the flask comprises a light-transmitting body 1, the body 1 having a substantially rectangular parallelepiped shape with smooth surfaces and possibly curved transitions at the corners. The height of the bottle body 1 is much smaller than the length or width, and the whole body is flat. The inside of the bottle body 1 can contain a culture solution and be used for culturing strains.
Preferably, the bottle body 1 is made of a suitable polymer material, typically plastic, and optionally glass, rubber or other suitable materials may be used.
Preferred data for vial 1 are 8cm in length, 5cm in width, 0.5cm in height, and about 20ml of solvent. In practice, the bottle body 1 can be enlarged or reduced in an appropriate equal ratio, but the height thereof still needs to be as small as possible, so that when the length and width of the bottle body 1 are enlarged in accordance with a certain ratio, the height thereof is enlarged in a ratio not larger than the ratio or smaller.
Since the height of the bottle body 1 is small, the whole bottle is not easy to clean, the bottle body 1 is generally selected to be a disposable sterile bottle body 1, and the bottle body 1 can be discarded after use.
When the bottle body 1 is used for culturing and counting strains, because the height of the bottle body 1 is small, the overlapping of the strains under the same plane can be reduced as much as possible, the concave-convex condition is avoided, and the sight line cannot have errors. In addition, the height of bottle 1 is less then can count the bacterial under a plane, and need not carry out extra upset, and the suspension bacterial that cultivates in the surface test tube takes place to remove and leads to missing to remember or the condition of remembering more, has guaranteed the accuracy of count.
A protruding bottle mouth 2 is arranged on the side surface of the bottle body 1, and a detachable bottle cap 3 is arranged on the bottle mouth 2. In order to prevent the liquid in the bottle body 1 from easily leaking out of the bottle through the bottle opening 2, the bottle opening 2 is provided to extend obliquely upward. Wherein the bottle cap 3 is cylindrical and has a considerable length, covering the bottle mouth 2 and approaching the bottle body 1. In addition, the outer periphery of the bottle mouth 2 and the inner periphery of the bottle cap 3 are respectively provided with corresponding threads so as to be matched for sealing connection.
In order to reduce the amount of oxygen in the bottle body 1 when culturing and counting anaerobic bacteria, a closed cap may be selected as the bottle cap 3 and the bottle body 1 may be filled with a liquid as much as possible.
When the aerobic bacteria are cultured and counted, in order to ensure that clean oxygen can smoothly enter the bottle body 1, the air filter element cover can be selected to be used as the bottle cap 3, and a certain supply space is reserved in the bottle body 1.
Preferably, a volume scale 4 is engraved on the side of the body 1, and the total volume of the solution in the body 1 can be read through the volume scale 4 to accurately adjust the amount of the solution to be added.
Preferably, when the amount of the bacterial species is large, the division lines 5 may be drawn on the bottle body 1, and the division lines 5 divide the surface of the bottle body 1 into a plurality of grids having similar areas. The dividing lines 5 are generally drawn on the bottom surface of the bottle body 1, but can be smoothly observed from the top surface of the bottle body 1 even in the presence of a solution and a strain. And if the solution and the strains have larger obstruction to the sight, the dividing lines 5 can be carved on the top surface of the bottle body 1. During counting, the bottle body 1 can be inverted and mixed evenly, the number of each strain in one or more grids can be counted, and the total amount of the strain can be estimated according to the volume in the bottle corresponding to each grid.
More preferably, the dividing lines 5 may be nine-square grids, or may be an appropriate number of grids such as sixteen grids or twelve grids according to actual circumstances.
What has been described above are only some embodiments of the invention. For those skilled in the art, without departing from the inventive concept, several modifications and improvements can be made, which are within the scope of the invention.
Claims (9)
1. A novel strain liquid counting culture bottle is characterized in that: the bottle comprises a bottle body (1) with a smooth surface, wherein the length of the bottle body (1) is 8cm, the width of the bottle body is 5cm, the height of the bottle body is 0.5cm, a protruding bottle opening (2) is arranged on the side surface of the bottle body (1), a cylindrical bottle cap (3) is installed on the bottle opening (2), and the bottle opening (2) extends towards the upper side in an inclined mode.
2. A novel liquid counting culture flask for strains according to claim 1, wherein: the length and width of the bottle body (1) are enlarged according to a scale, and the height is enlarged not more than the scale.
3. A novel liquid counting culture flask for strains according to claim 1, wherein: the bottle body (1) is made of plastic.
4. A novel liquid counting culture flask for strains according to claim 1, wherein: the bottle body (1) is made of glass or rubber.
5. A novel liquid counting culture flask for strains according to claim 1, wherein: the bottle body (1) is a disposable aseptic bottle.
6. A novel liquid counting culture flask for strains according to claim 1, wherein: the bottle cap (3) is a closed cap.
7. A novel liquid counting culture flask for strains according to claim 1, wherein: the bottle cap (3) is an air filter element cap.
8. A novel liquid counting culture flask for strains according to claim 1, wherein: the outer periphery of the bottle mouth (2) and the inner periphery of the bottle cap (3) are respectively provided with corresponding threads.
9. A novel liquid counting culture flask for strains according to claim 1, wherein: the side of the bottle body (1) is provided with volume scale marks (4).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202021592293.4U CN213475975U (en) | 2020-08-04 | 2020-08-04 | Novel strain liquid counting culture bottle |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202021592293.4U CN213475975U (en) | 2020-08-04 | 2020-08-04 | Novel strain liquid counting culture bottle |
Publications (1)
Publication Number | Publication Date |
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CN213475975U true CN213475975U (en) | 2021-06-18 |
Family
ID=76360628
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN202021592293.4U Expired - Fee Related CN213475975U (en) | 2020-08-04 | 2020-08-04 | Novel strain liquid counting culture bottle |
Country Status (1)
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CN (1) | CN213475975U (en) |
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2020
- 2020-08-04 CN CN202021592293.4U patent/CN213475975U/en not_active Expired - Fee Related
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Legal Events
Date | Code | Title | Description |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210618 |