CN213416847U - But high flux drawer type suction filtration plasmid purification device - Google Patents
But high flux drawer type suction filtration plasmid purification device Download PDFInfo
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- CN213416847U CN213416847U CN202022249249.XU CN202022249249U CN213416847U CN 213416847 U CN213416847 U CN 213416847U CN 202022249249 U CN202022249249 U CN 202022249249U CN 213416847 U CN213416847 U CN 213416847U
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Abstract
The utility model discloses a but suction filtration plasmid purification device of high flux drawer type, including the purification cabinet, the upper end of purification cabinet is provided with two sets of draw-in grooves, the upper end of purification cabinet is provided with the glass board, the both sides fixedly connected with fixture block of glass board, and the fixture block agrees with mutually with draw-in groove size shape, the upper end of glass board is provided with the first small opening of twelve groups, and twelve groups first small openings are on average arranged into two rows, the front end of purification cabinet is provided with the drawer, the bottom of drawer is provided with multiunit second small opening, and the position one-to-one of position and first small opening of second small opening. The utility model discloses in, through setting up twelve first small openings of group, can place 12 nucleic acid purification post simultaneous workings simultaneously, possible higher flux. One AxyVac II Vacuum Manual device can be used for placing 5 nucleic acid purification columns with the diameter of 3cm at most, while the device can be used for placing 12 nucleic acid purification columns, and the device occupies small space and does not occupy space.
Description
Technical Field
The utility model relates to a genetic engineering field especially relates to a but suction filtration plasmid purification device of high flux drawer type.
Background
The extraction and purification of plasmids from Escherichia coli is the basic molecular experiment operation, and the plasmid small extraction and the plasmid large extraction are distinguished according to the amount of plasmids needing to be extracted, and the plasmid large extraction generally refers to the extraction and purification of plasmid amount more than 50 ug. The invention mainly aims at the plasmid big extraction. In the existing plasmid large-scale extraction, an anion exchange column is generally used for purifying escherichia coli supernatant subjected to alkaline lysis, plasmid DNA is negatively charged and is adsorbed and combined by anion exchange column packing in a low-salt environment, and after impurities such as RNA and protein are removed by impurity washing liquid with different salt concentrations, the impurities are eluted by eluent with high salt concentration. In order to produce plasmid DNA in high throughput, a plurality of DNA fragments are simultaneously prepared, and an apparatus for placing an anion exchange column is required. At present, a common purifying device is provided with an iron stand, and only one purifying device can be placed.
The nucleic acid purification column can be fixed by using an iron support, and the column passing speed can be increased by externally connecting a suction filtration device, so that the defects of low flux, only one purification and large occupied area of a laboratory bench are overcome; 10-20 nucleic acid purification posts can be placed simultaneously to AxyVac II Vacuum Manual device that uses AXGEN, can external suction filter device, and the experiment can be accelerated in the stage of loading a sample and washing miscellaneous stage, but need move the nucleic acid purification post to the centrifuging tube of collection in the elution stage, and the operation is loaded down with trivial details relatively. In addition, because of the small volume, a plurality of relatively large nucleic acid purification columns cannot be placed at the same time, and most of the nucleic acid purification columns for plasmid large-scale extraction have a diameter of more than 2 cm. Therefore, the amount of plasmid extracted by the secondary device is also reduced.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a high-flux drawer type suction filtration plasmid purification device.
In order to achieve the above purpose, the utility model adopts the following technical scheme: a high-flux drawer type suction filtration plasmid purification device comprises a purification cabinet, wherein two groups of clamping grooves are formed in the upper end of the purification cabinet, a glass plate is arranged at the upper end of the purification cabinet, clamping blocks are fixedly connected to two sides of the glass plate, the clamping blocks are matched with the clamping grooves in size and shape, twelve groups of first leakage holes are formed in the upper end of the glass plate, and the twelve groups of first leakage holes are evenly arranged in two rows;
the front end of the purification cabinet is provided with a drawer, the bottom end of the drawer is provided with a plurality of groups of second leakage holes, and the positions of the second leakage holes correspond to the positions of the first leakage holes one to one.
As a further description of the above technical solution:
the front end of the drawer is fixedly connected with a handle, and the handle is positioned in the center of the front end of the drawer.
As a further description of the above technical solution:
the first leak hole is a conical hole, and the aperture of the lower part of the first leak hole is slightly smaller than that of the upper part of the first leak hole.
As a further description of the above technical solution:
the inner wall both sides of purification cabinet all fixedly connected with support frame, and drawer and support frame sliding connection.
As a further description of the above technical solution:
the rear end fixedly connected with waste liquid of purification cabinet is external to manage, and the waste liquid is external to manage and is communicated with each other with the purification cabinet, and purification cabinet is located the lower extreme of drawer with the interface that the waste liquid is external to manage.
As a further description of the above technical solution:
one side fixedly connected with connecting pipe of purification cabinet, and the connecting pipe have with the interface of purification cabinet be located the upper end of drawer, the lower extreme of glass board.
As a further description of the above technical solution:
one end of the connecting pipe is fixedly connected with a multi-way pipe, one end of the multi-way pipe is fixedly connected with a suction filtration pump, and the suction filtration pump is communicated with the purification cabinet through the multi-way pipe and the connecting pipe.
As a further description of the above technical solution:
the main material of the purification cabinet and the drawer is acrylic plates.
The utility model discloses following beneficial effect has:
1. the utility model discloses a set up the first small opening of twelve groups, can place 12 nucleic acid purification post simultaneous workings simultaneously, possible higher flux. One AxyVac II Vacuum Manual device can be used for placing 5 nucleic acid purification columns with the diameter of 3cm at most, while the device can be used for placing 12 nucleic acid purification columns, and the device occupies small space and does not occupy space.
2. The utility model can place nucleic acid purification columns with different specifications through the arranged glass plate, and the device of the invention can replace different glass plates, thereby corresponding to nucleic acid purification columns with different specifications. And is more flexible.
3. The utility model discloses a drawer that sets up makes the device can be applied to the appearance of going up, washes miscellaneous, the elution stage. Only the centrifuge tube rack needs to be put into a drawer before the elution stage. After elution, the drawer is taken out, the operation is simple and convenient, the nucleic acid purification column does not need to be moved, and the experiment time is saved.
4. The utility model discloses a multi-ported tube that sets up can make a plurality of the device establish ties to a suction filtration pump, through the purification speed of the nucleic acid purification post on the suction filtration pump acceleration first small opening, makes the production flux improve greatly.
Drawings
FIG. 1 is a partial perspective view of a high-throughput drawer-type suction filtration plasmid purification device provided by the present invention;
FIG. 2 is a top view of a high throughput drawer-type vacuum filtration plasmid purification apparatus of the present invention;
FIG. 3 is a front view of a high-throughput drawer-type suction filtration plasmid purification apparatus according to the present invention;
FIG. 4 is a right side view of a high throughput drawer-type suction filtration plasmid purification apparatus according to the present invention;
FIG. 5 is a cross-sectional view of a high-throughput drawer-type suction filtration plasmid purification device provided by the present invention.
Illustration of the drawings:
1. a glass plate; 2. a first leak hole; 3. a clamping block; 4. a connecting pipe; 5. a drawer; 6. a second leak hole; 7. a handle; 8. a purification cabinet; 9. the waste liquid is externally connected with a pipe; 10. a support frame; 11. a card slot; 12. a multi-pass tube; 13. and (4) a suction filtration pump.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1-5, the present invention provides an embodiment: the utility model provides a but high flux drawer type suction filtration plasmid purification device, includes purification cabinet 8, and the upper end of purification cabinet 8 is provided with two sets of draw-in grooves 11, and the upper end of purification cabinet 8 is provided with glass board 1, and glass board 1's both sides fixedly connected with fixture block 3, and fixture block 3 agrees with draw-in groove 11 size shape mutually, can change different glass board 1 to correspond the nucleic acid purification post of different specifications. More nimble, the upper end of glass board 1 is provided with the first small opening 2 of twelve groups, and twelve groups first small opening 2 are on average arranged into two rows, and twelve groups first small opening 2 can place 12 nucleic acid purification post simultaneous workings simultaneously, can accomplish higher flux, and first small opening 2 is the bell mouth, and the aperture slightly is less than above aperture below, can be better fix nucleic acid purification post, prevents that nucleic acid purification post from droing.
The front end of purification cabinet 8 is provided with drawer 5, the bottom of drawer 5 is provided with multiunit second small opening 6, and the position one-to-one of second small opening 6 and first small opening 2, the rear end fixedly connected with waste liquid of purification cabinet 8 is external to be taken over 9, and the waste liquid is external to be taken over 9 and purification cabinet 8 and communicates with each other, and purification cabinet 8 is external to be taken over 9 with the waste liquid interface and is located the lower extreme of drawer 5, can place the centrifuge tube support in the drawer 5, before the elution stage with centrifuge tube support put into in drawer 5 can. After elution, the drawer 5 is taken out, the operation is simple and convenient, the nucleic acid purification column does not need to be moved, the experimental time is saved, waste liquid generated in other stages directly leaks through the second leakage hole 6, and finally the waste liquid is discharged to a waste liquid pool from the waste liquid external connecting pipe 9.
The front end of the drawer 5 is fixedly connected with a handle 7, the handle 7 is positioned at the center of the front end of the drawer 5, the drawer 5 is convenient to pull out, both sides of the inner wall of the purification cabinet 8 are fixedly connected with a support frame 10, the drawer 5 is in sliding connection with the support frame 10, the position of the drawer 5 can be fixed by the support frame 10, one side of the purification cabinet 8 is fixedly connected with a connecting pipe 4, the connecting pipe 4 is provided with an interface with the purification cabinet 8 and is positioned at the upper end of the drawer 5, the lower end of the glass plate 1, one end of the connecting pipe 4 is fixedly connected with a multi-way pipe 12, one end of the multi-way pipe 12 is fixedly connected with a suction pump 13, the suction pump 13 is communicated with the purification cabinet 8 through the multi-way pipe 12 and the connecting pipe 4, a plurality of the devices can be connected in series to one suction pump 13 through the multi-way pipe 12, the purification speed of the nucleic acid purification column on the first leak 2, the acrylic plate has low cost, corrosion resistance and easy cleaning.
The working principle is as follows: firstly, replacing glass plates 1 with first leak holes 2 with different apertures according to the sizes of different nucleic acid purification columns, inserting a plurality of groups of nucleic acid purification columns into the first leak holes 2, pouring supernatant into the column tubes of the nucleic acid purification columns during sample loading, opening a suction pump 13, forming negative pressure inside a purification cabinet 8, enabling supernatant liquid to flow through the nucleic acid purification columns, enabling flow-through substances to firstly pass through second leak holes 6 below a drawer 5 and drip to the bottom layer of the purification cabinet 8, then enabling the flow-through substances to enter a waste liquid pool through a waste liquid external connection tube 9 at the bottom layer, pouring impurity washing buffer liquid into the column tubes of the nucleic acid purification columns during an impurity washing step, opening the suction pump 13, enabling the purification cabinet 8 to form negative pressure, enabling the impurity washing liquid to flow through the nucleic acid purification columns, enabling the flow-through substances to enter the waste liquid pool through the waste liquid external connection tube 9, firstly taking out the drawer 5, placing the centrifugal tube frames and centrifugal tubes into 12 holes during an elution step, and pouring eluted buffer liquid, open the suction filtration pump 13, the inside negative pressure that forms of device, the liquid stream of elution passes through the nucleic acid purification post, and in the thing entering centrifuge tube of flowing through, the collection work has been accomplished, if need multiunit device simultaneous working, only need be connected to the connecting pipe 4 of multiunit device on same group's multi-ported tube 12, can realize the suction filtration simultaneously of multiunit device through a set of suction filtration pump 13.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (8)
1. The utility model provides a but high flux drawer type suction filtration plasmid purification device, includes purification cabinet (8), its characterized in that: the upper end of the purification cabinet (8) is provided with two groups of clamping grooves (11), the upper end of the purification cabinet (8) is provided with a glass plate (1), two sides of the glass plate (1) are fixedly connected with clamping blocks (3), the clamping blocks (3) are matched with the clamping grooves (11) in size and shape, the upper end of the glass plate (1) is provided with twelve groups of first leakage holes (2), and the twelve groups of first leakage holes (2) are evenly arranged in two rows;
the front end of the purification cabinet (8) is provided with a drawer (5), the bottom end of the drawer (5) is provided with a plurality of groups of second leakage holes (6), and the positions of the second leakage holes (6) are in one-to-one correspondence with the positions of the first leakage holes (2).
2. The high-throughput drawer-type suction filtration plasmid purification device of claim 1, wherein: the front end of the drawer (5) is fixedly connected with a handle (7), and the handle (7) is positioned in the center of the front end of the drawer (5).
3. The high-throughput drawer-type suction filtration plasmid purification device of claim 1, wherein: the first leak hole (2) is a conical hole, and the aperture of the lower part is slightly smaller than that of the upper part.
4. The high-throughput drawer-type suction filtration plasmid purification device of claim 1, wherein: the inner wall of the purification cabinet (8) is fixedly connected with support frames (10) on two sides, and the drawer (5) is connected with the support frames (10) in a sliding mode.
5. The high-throughput drawer-type suction filtration plasmid purification device of claim 1, wherein: the rear end fixedly connected with waste liquid of purification cabinet (8) is external to manage (9), and the waste liquid is external to manage (9) and is communicated with each other with purification cabinet (8), and purification cabinet (8) are external to manage the interface of (9) with the waste liquid and are located the lower extreme of drawer (5).
6. The high-throughput drawer-type suction filtration plasmid purification device of claim 1, wherein: one side fixedly connected with connecting pipe (4) of purification cabinet (8), and connecting pipe (4) have with the interface of purification cabinet (8) be located the upper end of drawer (5), the lower extreme of glass board (1).
7. The high-throughput drawer-type suction filtration plasmid purification device of claim 6, wherein: one end of the connecting pipe (4) is fixedly connected with a multi-way pipe (12), one end of the multi-way pipe (12) is fixedly connected with a suction and filtration pump (13), and the suction and filtration pump (13) is communicated with the purification cabinet (8) through the multi-way pipe (12) and the connecting pipe (4).
8. The high-throughput drawer-type suction filtration plasmid purification device of claim 1, wherein: the main material of the purification cabinet (8) and the drawer (5) is acrylic plates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022249249.XU CN213416847U (en) | 2020-10-12 | 2020-10-12 | But high flux drawer type suction filtration plasmid purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022249249.XU CN213416847U (en) | 2020-10-12 | 2020-10-12 | But high flux drawer type suction filtration plasmid purification device |
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| Publication Number | Publication Date |
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| CN213416847U true CN213416847U (en) | 2021-06-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022249249.XU Active CN213416847U (en) | 2020-10-12 | 2020-10-12 | But high flux drawer type suction filtration plasmid purification device |
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| CN (1) | CN213416847U (en) |
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