CN213012895U - Ultrasonic crusher - Google Patents
Ultrasonic crusher Download PDFInfo
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- CN213012895U CN213012895U CN202021712350.8U CN202021712350U CN213012895U CN 213012895 U CN213012895 U CN 213012895U CN 202021712350 U CN202021712350 U CN 202021712350U CN 213012895 U CN213012895 U CN 213012895U
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- 238000012545 processing Methods 0.000 claims abstract description 30
- 238000012360 testing method Methods 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 230000005855 radiation Effects 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 15
- 238000002525 ultrasonication Methods 0.000 abstract description 9
- 239000002699 waste material Substances 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 48
- 239000012472 biological sample Substances 0.000 description 8
- 210000003781 tooth socket Anatomy 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000000498 cooling water Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- 235000019047 Crassocephalum crepidioides Nutrition 0.000 description 1
- 244000287353 Crassocephalum crepidioides Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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Abstract
The utility model discloses an ultrasonograph, ultrasonograph includes ultrasonic transducer (1), profile of tooth energy collector (2) and sample processing groove (3), wherein, ultrasonic transducer (1) sets up in the lower part of sample processing groove 3, and profile of tooth energy collector (2) set up the inside at sample processing groove (3) to gather the acoustic energy. The tooth-shaped energy concentrator (2) comprises a plate-shaped part and a tooth-shaped part, the bottom surface of the plate-shaped part is fixedly connected with the inner bottom surface of the sample processing groove (3), the number of teeth of the tooth-shaped part is two or the tooth-shaped part is a tooth-shaped array consisting of more than two teeth, the cross section of each tooth is triangular, rectangular or trapezoidal, and a chamfer or fillet structure is arranged on each tooth. The utility model provides an ultrasonication appearance utilizes the reflection of sound wave between test tube holder (or liquid level) and profile of tooth energy collector and the effect that gathers of profile of tooth structure, improves the local sound intensity of tooth's socket position, has reduced the waste of sound energy, has improved the utilization ratio.
Description
Technical Field
The utility model relates to a biological sample processing apparatus technical field, concretely relates to profile of tooth structure ultrasonication appearance of non-contact.
Background
Under normal temperature and normal pressure, ultrasonic waves emitted by the ultrasonic transducer are transmitted in liquid, and when the amplitude of the sound waves is larger than a cavitation threshold value, negative pressure is formed in the liquid, so that acoustic cavitation is excited. When acoustic cavitation occurs, a large number of bubbles vibrate and collapse in the liquid: micro-flow and mechanical friction are generated around the cavitation bubbles when the cavitation bubbles vibrate, micro-jet flow and shock wave equivalent effects are generated when the cavitation bubbles collapse, and the effects have certain destructive effects on biological particles, so that the cavitation bubbles can be used for crushing biological samples.
Two common approaches to ultrasonication are: one is contact ultrasonic crushing, in which an ultrasonic transducer with an amplitude transformer is inserted into a sample container for crushing. Another is non-contact ultrasonication: and arranging an ultrasonic transducer at the bottom of the water tank, and putting the test tube containing the sample in the ultrasonic water tank for sample crushing. The non-contact ultrasonication has the advantages that multiple samples can be processed simultaneously, and the test tube can be sealed in the processing process, so that the pollution of the samples is avoided.
Generally, the capacity and the sound intensity of ultrasonic crushing are related to the frequency, and the effect of ultrasonic crushing can be directly improved by increasing the sound intensity of the ultrasonic. However, for the conventional ultrasonic crusher, most of the sound energy is distributed in the whole container, and if the local sound intensity at the crushing position is increased, the sound intensity in the whole container needs to be increased, so that the waste of sound energy is caused, and the loss of equipment is increased.
Therefore, it is necessary to provide an ultrasonograph which can efficiently collect sound waves, improve the utilization efficiency of sound energy, and have a good ultrasonograph effect.
SUMMERY OF THE UTILITY MODEL
In order to overcome the above problems, the present inventors have conducted a keen study to design a tooth profile structured ultrasonograph, which utilizes the reflection of ultrasonic waves between a test tube holder and a tooth profile structure and the energy-gathering effect of the tooth profile structure, improves the local sound intensity of a sample position, and realizes ultrasonication under high sound energy utilization rate, thereby completing the present invention.
In particular, the utility model aims to provide an ultrasonic crusher with a tooth-shaped structure, wherein the ultrasonic crusher comprises an ultrasonic transducer 1, a tooth-shaped energy concentrator 2 and a sample processing groove 3,
wherein the ultrasonic transducer 1 is arranged at the lower part of the sample processing groove 3,
a toothed concentrator 2 is provided inside the sample processing well 3 to concentrate the acoustic energy.
Wherein, the ultrasonic transducer 1 is one or more, and the radiation surface thereof is fixedly connected with the bottom surface of the sample processing groove 2.
Wherein the toothed concentrator 2 comprises a plate-shaped portion and a toothed portion,
the bottom surface of the plate-shaped part is fixedly connected with the inner bottom surface of the sample processing groove 3, and the number of the teeth-shaped parts is multiple.
The number of teeth of the tooth-shaped part is two or the tooth-shaped part is a tooth-shaped array consisting of more than two teeth.
The tooth of the tooth-shaped part is triangular, rectangular or trapezoidal in cross section, and a chamfer or fillet structure is arranged on the tooth.
When the tooth-shaped part is in a tooth-shaped array structure consisting of more than two teeth, the cross sections of the teeth are the same.
Wherein, the upper part of the tooth-shaped energy concentrator 2 and the inside of the sample processing groove 3 are also provided with a test tube bracket 4 for fixing the sample containing tube 5 and reflecting the ultrasonic waves emitted from the upper surface of the tooth-shaped energy concentrator 2.
Wherein the test tube holder 4 comprises a vertical plate and a horizontal plate, both having sound reflection capability;
an opening is arranged on the horizontal plate to place the sample containing tube 5.
Wherein, a gap is arranged between the bottom of the sample containing pipe 5 and the bottom of the tooth groove.
Wherein, the sample in the sample containing pipe 5 is liquid, and the height of the liquid level is not higher than the vertical height of the tooth socket.
The utility model discloses the beneficial effect who has includes:
(1) the utility model provides an ultrasonic crusher, easy operation, wide application scope, strong versatility;
(2) the utility model provides an ultrasonic crusher, utilize the reflection of sound wave between test tube support and profile of tooth energy concentrator and the energy-collecting effect of profile of tooth structure, improve the local sound intensity in tooth's socket position, reduced the waste of sound energy, improved the utilization ratio;
(3) the utility model provides an ultrasonication appearance, the sound current that produces in the upper portion region of tooth's socket can play the stirring effect to the liquid in the sample splendid attire pipe, improves the broken even degree of sample.
Drawings
Fig. 1 is a schematic view showing an overall structure of a tooth-shaped ultrasonic crusher according to a preferred embodiment of the present invention;
fig. 2 is a schematic sectional view of a ultrasonic crusher having a tooth structure according to a preferred embodiment of the present invention.
The reference numbers illustrate:
1-an ultrasound transducer;
2-a toothed concentrator;
3-a sample treatment tank;
4-test tube holder;
5-sample containing tube.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and embodiments. The features and advantages of the present invention will become more apparent from the description. In which, although various aspects of the embodiments are shown in the drawings, the drawings are not necessarily drawn to scale unless specifically indicated.
The inventor researches and discovers that the tooth-shaped structure has better energy accumulation effect and can effectively reduce the loss of sound waves, therefore, the utility model provides an ultrasonic crusher with the tooth-shaped structure, the ultrasonic crusher comprises an ultrasonic transducer 1, a tooth-shaped energy accumulator 2 and a sample processing groove 3,
wherein the ultrasonic transducer 1 is arranged at the lower part of the sample processing groove 3,
a toothed concentrator 2 is provided inside the sample processing well 3 to concentrate the acoustic energy, as shown in fig. 1.
In the present invention, the ultrasonic crusher with a tooth-shaped structure is suitable for processing biological samples, such as cells, biological tissues, nucleotide fragments, etc.
According to a preferred embodiment of the present invention, the radiation surface of the ultrasonic transducer 1 is fixedly connected to the bottom surface of the sample processing groove 2, and the fixed connection may be a connection method commonly used in the art, such as adhesion.
In the present invention, the ultrasonic transducer is used to convert the input electric power into mechanical power, i.e., ultrasonic waves, and then transmit the mechanical power. The ultrasound transducers commonly used in the art may be selected, such as: a sandwich piezoelectric transducer.
The radiation surface of the ultrasonic transducer is fixedly connected with the bottom surface of the sample processing groove, so that the vibration of the ultrasonic transducer excites the vibration of the tooth-shaped energy concentrator in the sample processing groove.
In a further preferred embodiment, the ultrasonic transducers 1 may be one or more, and the models may be the same or different.
The number of the ultrasonic transducers is positively correlated with the working power of the ultrasonic transducers, and the working power is higher when the number is larger. The utility model discloses in handle the target differently according to ultrasonic transducer, set up different operating power.
In a further preferred embodiment, the operating frequency of the ultrasonic transducer is between 16kHz and 10 MHz.
In the present invention, the operating frequency of the ultrasonic transducer can be selected according to the requirement, for example, the frequency of 20kHz to 3MHz can be selected.
According to a preferred embodiment of the invention, the toothed concentrator 2 comprises a plate-shaped part and a toothed part, as shown in figure 2,
the bottom surface of the plate-like portion is fixedly connected to the inner bottom surface of the sample treatment well 3 by means of a conventional connection means, such as adhesion.
In a further preferred embodiment, the number of teeth of the tooth-like portion is plural,
preferably, the number of teeth is two, or
The tooth-shaped part is a tooth-shaped array formed by more than two teeth.
The utility model discloses in, the number of teeth of dentate part can be adjusted as required, forms the tooth's socket between a plurality of teeth.
In a further preferred embodiment, the teeth of the tooth-like portion have a triangular, rectangular or trapezoidal cross-sectional shape.
Preferably, a chamfer or fillet structure is provided on the teeth.
According to a preferred embodiment of the present invention, when the tooth portion has a tooth array structure including two or more teeth, the plurality of teeth have the same cross-sectional shape.
In a further preferred embodiment, when the tooth portion has a tooth array structure of two or more teeth, the pitch between adjacent teeth is the same.
Wherein the pitch between the adjacent teeth refers to a distance between tooth tops of the adjacent teeth.
The present invention relates to a method for producing a tooth-shaped energy concentrator, and more particularly to a method for producing a tooth-shaped energy concentrator. In order to ensure the emission effect of the ultrasonic waves, the toothed concentrator is preferably arranged directly above the ultrasonic transducer.
Preferably, when the number of the ultrasonic transducers is 1, the ultrasonic transducers are arranged coaxially with the tooth-shaped transducer;
when the ultrasonic transducer is a plurality of ultrasonic transducers, the ultrasonic transducers are uniformly distributed at the lower part of the tooth-shaped transducer.
The utility model discloses in, the length of profile of tooth energy collector (the length of platelike portion), the figure and the size of tooth can be adjusted according to actual demand, and ultrasonic transducer's figure, size, mode of arrangement and operating frequency etc. also can select according to actual demand.
For example: in fig. 1 and 2, the tooth-shaped energy concentrator is provided with four teeth to form three tooth sockets, three rows of samples can be processed simultaneously, and two transducers of the same type are arranged below the tooth-shaped energy concentrator to perform excitation.
According to a preferred embodiment of the present invention, the upper portion of the tooth-shaped energy concentrator 2, the inside of the sample processing groove 3, and the test tube holder 4 are further provided to fix the sample holding tube 5 and reflect the ultrasonic wave emitted from the upper surface of the tooth-shaped energy concentrator 2.
Wherein, the test tube bracket can be movably or fixedly arranged in the sample processing groove.
In a further preferred embodiment, the test tube holder 4 comprises a vertical plate and a horizontal plate, both having acoustic reflection capability;
preferably comprising two oppositely disposed vertical plates and a horizontal plate connected between the two vertical plates,
wherein, the vertical plates are arranged at both sides of the tooth-shaped energy concentrator 2 and arranged along the direction of increasing the number of teeth;
the horizontal plate is disposed above the teeth as shown in fig. 2.
Wherein a gap is formed between the vertical plate and the tooth-shaped energy concentrator.
In a further preferred embodiment, openings are provided in the horizontal plate for the placement of sample receiving tubes 5;
preferably, the arrangement of the openings on the horizontal plate is matched with the arrangement of the tooth sockets, so that the sample holding tube 5 is vertically arranged in the tooth sockets, and the corresponding openings above each tooth socket can be one or more, and are preferably distributed in an in-line manner.
The utility model discloses in, ultrasonic wave is from gathering the ware upper surface transmission and by test tube holder's horizontal plate and vertical board reflection to produce great local standing wave sound field in the tooth's socket, improved the utilization ratio of sound energy, the cavitation effect that utilizes big acoustic pressure to produce simultaneously carries out the breakage to the biological sample.
According to the utility model relates to a preferred embodiment, sample splendid attire pipe 5 can be test tube, centrifuging tube etc. and its bottom has the clearance with flute bottom portion, and the clearance size is 0mm ~ 10mm, nevertheless does not include 0 mm.
The present inventors found that the ultrasonic crushing effect is weakened by an excessively large clearance, and therefore the distance between the sample-holding tube and the bottom of the tooth socket is set to the above range.
Wherein, the upper portion of sample splendid attire pipe is fixed on test tube holder, sets up the clearance fit that proper height realized sample splendid attire socle bottom and tooth tank bottom through test tube holder. The sample holding tube and the test tube holder can be matched by the common technology in the field, for example, the sample holding tube adopts a centrifuge tube.
Preferably, the distance between the outer wall of the sample containing tube and the adjacent teeth is 2mm to 10 mm.
In a further preferred embodiment, the sample-containing tube 5 is located on the central plane of the tooth grooves, which is perpendicular to the direction in which the number of teeth increases.
In a further preferred embodiment, the sample in the sample holding tube 5 is liquid, and the liquid level is not higher than the vertical level of the gullet.
According to a preferred embodiment of the present invention, the sample processing tank 3 is further provided with a water inlet end and a water outlet end for the inflow and discharge of cooling water;
wherein the cooling water is used for cooling the biological sample to be processed.
Ultrasonication appearance of profile of tooth structure, utilize the profile of tooth to gather the effect to the sound wave, produce great acoustic pressure in the tooth's socket position, improved the utilization efficiency of sound energy, the sound current that produces simultaneously in the upper portion region of tooth's socket can play stirring effect to the liquid in the test tube, improves the broken even degree of sample.
The working process of the ultrasonic crusher with the tooth-shaped structure is as follows: the number, the arrangement mode, the working frequency, the power and the like of the ultrasonic transducers, the number, the size, the shape and the like of teeth of the tooth-shaped energy concentrator are set according to actual requirements; then, a proper amount of biological samples to be processed are placed in the sample containing tube and fixed in the test tube support, so that the samples are positioned in the grooves, the ultrasonic transducer is started to carry out ultrasonic crushing work, the vibration of the ultrasonic transducer excites the vibration of the tooth-shaped energy concentrator, ultrasonic waves are emitted from the upper surface of the energy concentrator and are reflected by the test tube support, so that standing waves are formed between the test tube support and the energy concentrator, larger sound pressure can be generated in the tooth space area, and the biological samples are crushed by utilizing the cavitation effect generated by the large sound pressure; in the working process, cooling water is pumped in from one end of the sample processing groove by a water pump, and the cooling water is discharged from the other end of the sample processing groove so as to cool the processed biological sample.
Examples
The present invention is further described below by way of specific examples, which are only exemplary and do not limit the scope of the present invention in any way.
Example 1
In the embodiment, the cross section of the teeth of the tooth-shaped energy concentrator is triangular, the tooth height is 20mm, the tooth width is 10mm, the tooth space is 15mm, the length of the tooth-shaped energy concentrator is 60mm, 4 teeth are provided, 1 to 9 samples can be simultaneously crushed, the number of the ultrasonic transducers is 1, the model is HNC-4SS-3833 of Suzhou Hainan technology, the factory resonance frequency of the transducers is 33kHz and 67kHz, the maximum power is 60W, the active electric power of the transducers is 10W during actual work, and the working frequency is about 65.27 kHz.
Adopt the profile of tooth energy concentrator of above-mentioned structure to carry out the breakage to DNA sample (10.4kbp, the provision of zhhai Ebolo biotechnology limited company), broken total operating time 20min, ON/OFF is 30s/30s, runs the length distribution that the gluey method detected the DNA sample with PCR electrophoresis, obtains DNA fragment and has about 70% to distribute between the target length of 200bp-300bp, the result shows that the ultrasonication appearance of profile of tooth structure can effectively smash the DNA sample piece.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", etc. indicate the position or positional relationship based on the operation state of the present invention, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element indicated must have a specific position, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly specified or limited, the terms "connected" and "connected" are to be construed broadly, e.g., as meaning a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; the connection may be direct or indirect via an intermediate medium, and may be a communication between the two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The present invention has been described above in connection with preferred embodiments, which are merely exemplary and illustrative. On this basis, can be right the utility model discloses carry out multiple replacement and improvement, these all fall into the utility model discloses a protection scope.
Claims (8)
1. An ultrasonic crusher is characterized by comprising an ultrasonic transducer (1), a tooth-shaped energy concentrator (2) and a sample processing groove (3),
wherein the ultrasonic transducer (1) is arranged at the lower part of the sample processing groove (3),
the tooth-shaped energy concentrator (2) is arranged inside the sample processing groove (3) to concentrate sound energy;
the toothed concentrator (2) comprises a plate-shaped part and a toothed part,
the bottom surface of the plate-shaped part is fixedly connected with the inner bottom surface of the sample processing groove (3), and the number of teeth of the toothed part is multiple;
the upper part of the tooth-shaped energy concentrator (2) and the inside of the sample processing groove (3) are also provided with a test tube bracket (4) for fixing the sample containing tube (5) and reflecting ultrasonic waves emitted by the upper surface of the tooth-shaped energy concentrator (2).
2. The ultrasonograph according to claim 1, wherein the ultrasonic transducer (1) is one or more, and a radiation surface thereof is fixedly connected to a bottom surface of the sample processing well (3).
3. The ultrasonograph according to claim 1, wherein the number of teeth of the tooth portion is two or the tooth portion is a tooth array of two or more teeth.
4. The ultrasonograph according to claim 1, wherein the teeth of the tooth-like portion have a triangular, rectangular or trapezoidal cross-sectional shape, and a chamfer or fillet structure is provided on the teeth.
5. The ultrasonograph according to claim 4, wherein the teeth have the same cross-sectional shape when the tooth portion has a tooth array structure of two or more teeth.
6. The ultrasonograph according to claim 1, wherein the cuvette holder (4) comprises a vertical plate and a horizontal plate, each having acoustic reflection capability;
the horizontal plate is provided with an opening for placing a sample containing tube (5).
7. The ultrasonograph according to claim 6, wherein the sample holding tube (5) has a gap between the bottom of the sample holding tube and the bottom of the gullet.
8. The ultrasonograph according to claim 6, wherein the sample in the sample holding tube (5) is a liquid, and the liquid level is not higher than the vertical level of the gullet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021712350.8U CN213012895U (en) | 2020-08-17 | 2020-08-17 | Ultrasonic crusher |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021712350.8U CN213012895U (en) | 2020-08-17 | 2020-08-17 | Ultrasonic crusher |
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| Publication Number | Publication Date |
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| CN213012895U true CN213012895U (en) | 2021-04-20 |
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| CN202021712350.8U Active CN213012895U (en) | 2020-08-17 | 2020-08-17 | Ultrasonic crusher |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979122A (en) * | 2020-08-17 | 2020-11-24 | 珠海艾博罗生物技术股份有限公司 | Ultrasonic crusher with tooth-shaped structure |
-
2020
- 2020-08-17 CN CN202021712350.8U patent/CN213012895U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979122A (en) * | 2020-08-17 | 2020-11-24 | 珠海艾博罗生物技术股份有限公司 | Ultrasonic crusher with tooth-shaped structure |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: An ultrasonic crusher Granted publication date: 20210420 Pledgee: Zhuhai Branch of China Construction Bank Co.,Ltd. Pledgor: ZHUHAI APPLETREE BIOTECHNOLOGY CO.,LTD. Registration number: Y2024980013033 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |