CN212748736U - Multi-channel switching device for fluorescence detection based on Photodiode (PD) - Google Patents
Multi-channel switching device for fluorescence detection based on Photodiode (PD) Download PDFInfo
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- CN212748736U CN212748736U CN202021641309.6U CN202021641309U CN212748736U CN 212748736 U CN212748736 U CN 212748736U CN 202021641309 U CN202021641309 U CN 202021641309U CN 212748736 U CN212748736 U CN 212748736U
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Abstract
The invention discloses a multichannel switching device for fluorescence detection based on a Photodiode (PD), which comprises an excitation light LED, an excitation light optical filter, a filter wheel shell, an emission light optical filter and a photodiode detection module, wherein the excitation light LED is arranged on the excitation light filter; the exciting light LED emits exciting light with a specific wavelength, the exciting light is collimated by the collimating lens, the exciting light is further filtered to light waves with the specific wavelength by the exciting light filter, the test tube sample is irradiated by the light guide element, and a fluorescence signal is excited; the fluorescent signal passes through the light guide element, an invalid signal is filtered out through the emission light filter, and the fluorescent signal is focused on the photodiode detection module through the collimating lens to be converted into an electric signal; the filter component for switching specific wavelength by rotating a specific angle through the motor realizes a multiband detection function.
Description
Technical Field
The invention belongs to the field of molecular diagnosis, and relates to a multi-channel switching device for fluorescence detection based on a Photodiode (PD).
Background
Multiplex PCR (multiplex PCR), also called multiplex PCR or multiplex PCR, is a PCR reaction in which two or more pairs of primers are added to the same PCR reaction system to simultaneously amplify multiple nucleic acid fragments, and the reaction principle, reaction reagents and operation process are the same as those of ordinary PCR. Multiplex PCR (multiplex PCR), in which a plurality of pairs of primers and a plurality of fluorescent probes are added to a reaction system for a plurality of target DNAs by using the specificity of the combination of the primers and the fluorescent probes with the target DNAs, and the plurality of target DNAs are detected by one-time real-time fluorescent quantitative PCR. The method overcomes the defects of complicated operation, difficult quantification and easy pollution of the common PCR, improves the detection flux and reliability, and makes the real-time fluorescence quantitative PCR become practical. In multiplex PCR, in order to avoid the mutual mixing of fluorescence of different fluorescent probes, fluorescent reporter groups with different excitation wavelengths and detection wavelengths are selected to synthesize the fluorescent probes. This also puts the demand of multiple fluorescence channels on the fluorescence detection system of the real-time fluorescence quantitative PCR instrument.
Like a general Diode, a photodiode (Photo-Diode) is also a semiconductor device including a PN junction and has a unidirectional conductive characteristic. But it is not a rectifying element in the circuit, but a photoelectric sensing device that converts an optical signal into an electrical signal.
A photomultiplier tube (PMT), an important product in photon counting devices, is a light detection device with extremely high sensitivity and ultra-fast time response.
The CCD is a charge coupled device, which is a detecting element that uses charge to represent the signal size and transmits the signal in a coupling mode, has a series of advantages of self-scanning, wide sensing spectrum range, small distortion, small volume, light weight, low system noise, low power consumption, long service life, high reliability and the like, and can be made into an assembly with very high integration level.
At present, the domestic multi-fluorescence channel nucleic acid amplification detection technology mainly adopts PMT/CCD as a main core fluorescence detection element, and the scheme has the following defects:
the core elements such as PMT, CCD and the like mainly depend on an inlet;
the cost of core elements such as PMT and CCD is high, and the price is about 50 times that of a Photo-Diode (Photo-Diode).
Because the sensitivity of a photodiode (Photo-Diode) is low, a strong excitation light source is needed to detect an effective signal, and the current domestic multi-fluorescence channel nucleic acid amplification detection technology mainly adopts a white LED single light source for excitation and cannot meet the detection requirement of the photodiode (Photo-Diode).
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides a multi-channel switching device for fluorescence detection based on a Photodiode (PD). The excitation light sources with different wave bands can meet the detection requirements of the photodiode (Photo-Diode), thereby greatly reducing the overall cost of equipment and reducing the dependence on imported elements.
A multi-channel switching device for fluorescence detection based on Photodiodes (PDs), comprising:
the exciting light LED is an LED lamp with a specific wavelength;
the exciting light filter is used for further filtering invalid wave bands of the LED lamp to form clean exciting light with specific wavelength;
the filter wheel comprises one or more wave band excitation light filter fixing grooves, one or more wave band excitation light fixing grooves and one or more wave band emission light filter fixing grooves, and the excitation light filter fixing grooves and the excitation light fixing grooves are in one-to-one correspondence and are coaxially arranged;
the filter wheel shell is used for sealing the filter wheel in a darkroom to prevent the influence of external light;
an emission light filter for filtering the invalid portion of the fluorescence signal to form a clean fluorescence signal of a specific wavelength;
a photodiode detection module provided with a Photodiode (PD) for detecting a fluorescent signal incident through the emission filter.
Preferably, the excitation light filter fixing grooves of different wave bands of the filter wheel are arranged at equal angles.
Preferably, the specific waveband excitation light filter and the specific waveband emission light filter of the filter wheel are arranged in a 180-degree manner and are arranged in a matched mode.
Preferably, a collimating lens is arranged between the excitation light of the filter wheel and the excitation light filter.
Preferably, a collimating lens is arranged between the emission filter of the filter wheel and the photodiode detection module.
Preferably, an excitation light hole and a fluorescence signal hole are arranged above the filter wheel shell.
Preferably, a light guide element is arranged above the filter wheel shell.
Preferably, the multi-channel switching device comprises a motor drive assembly connected to the filter wheel.
Compared with the prior art, the gain effect of the invention is as follows:
greatly reducing the equipment cost.
Reducing the dependence on the inlet element.
Drawings
Fig. 1 is a cross-sectional view of the overall structure of a multi-channel switching device for fluorescence detection based on a Photodiode (PD).
Fig. 2 is a perspective view of the overall structure of a multi-channel switching device for fluorescence detection based on Photodiodes (PDs).
Fig. 3 is a perspective view of the inside of a multi-channel switching device for fluorescence detection based on Photodiodes (PDs).
Fig. 4 is a perspective view of the filter wheel.
FIG. 5 is a top view of the filter wheel.
Detailed Description
The technical embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.
As shown in fig. 1, the present invention discloses a multi-channel switching device for fluorescence detection based on Photodiode (PD), comprising: the device comprises an exciting light LED (1), an exciting light filter (2), a filter wheel (3), a filter wheel shell (4), an emitting light filter (5) and a photodiode detection module (6).
The excitation light LED (1) is provided with six excitation light LEDs (wavelength range 470nm to 692nm) as shown in FIG. 3.
The excitation light filter (2) is provided with six excitation light filters (wavelength range 470nm to 692nm) as shown in FIG. 3.
A filter wheel (3) comprising six excitation light filter (2) fixing grooves, six excitation light LED (1) fixing grooves, and six emission light filter (5) fixing grooves as shown in fig. 4; a collimating lens (7) is arranged between the exciting light and the exciting light filter; the exciting light filter fixing grooves correspond to the exciting light fixing grooves one by one and are coaxially arranged; the specific wavelength excitation light filter (2) and the specific wavelength emission light filter (5) are arranged in an angle of 180 degrees; the filters of different wavelength bands are equiangularly arranged as shown in fig. 5.
As shown in fig. 2, the filter wheel housing (4) is a sealed structure, and an excitation light hole and a fluorescence signal hole are disposed above the filter wheel housing and used for fixing the light guide element (9).
The photodiode detection module (6) is fixed on the lower side of the filter wheel shell (4) as shown in fig. 3, and the photodiode detection module (6) and the emission optical filter (5) are provided with collimating mirrors (8).
The operating principle of a multi-channel switching device for fluorescence detection based on Photodiodes (PD) will now be described in further detail with reference to fig. 1.
The exciting light LED (1) emits exciting light with a specific wavelength, the exciting light is collimated by the collimating lens (7), the exciting light is further filtered to light waves with the specific wavelength by the exciting light filter (2), and a test tube sample is irradiated by the light guide element (9) to excite a fluorescence signal. The fluorescent signal passes through a light guide element (9), invalid signals are filtered out through an emission light filter (5), and the fluorescent signal is focused on a photodiode detection module (6) through a collimating lens (8) and converted into an electric signal. The multi-channel switching device realizes a multi-band detection function through a filter assembly which is rotated by a motor (10) at a specific angle so as to switch specific wavelengths as shown in figure 3.
It is to be understood that: although the present invention has been described in detail, the present invention is only for the purpose of illustration and not limitation, and any invention that does not depart from the spirit of the present invention falls within the scope of the present invention.
Claims (8)
1. A multi-channel switching device for fluorescence detection based on Photodiodes (PDs), comprising:
the exciting light LED is an LED lamp with a specific wavelength;
the exciting light filter is used for further filtering invalid wave bands of the LED lamp to form clean exciting light with specific wavelength;
the filter wheel comprises one or more wave band excitation light filter fixing grooves, one or more wave band excitation light fixing grooves and one or more wave band emission light filter fixing grooves, and the excitation light filter fixing grooves and the excitation light fixing grooves are in one-to-one correspondence and are coaxially arranged;
the filter wheel shell is used for sealing the filter wheel in a darkroom to prevent the influence of external light;
an emission light filter for filtering the invalid portion of the fluorescence signal to form a clean fluorescence signal of a specific wavelength;
a photodiode detection module provided with a Photodiode (PD) for detecting a fluorescent signal incident through the emission filter.
2. The multi-channel switching device for Photodiode (PD) -based fluorescence detection according to claim 1, wherein the excitation light filter fixation slots of different wavelength bands of the filter wheel are equiangularly arranged.
3. The multi-channel switching device for Photodiode (PD) -based fluorescence detection as claimed in claim 1, wherein the specific wavelength band excitation light filter and the specific wavelength band emission light filter of the filter wheel are mounted at 180 ° in paired arrangement.
4. The multi-channel switching device for Photodiode (PD) -based fluorescence detection as claimed in claim 1, wherein a collimating lens is disposed between the excitation light and the excitation light filter of the filter wheel.
5. The multi-channel switching device for Photodiode (PD) -based fluorescence detection as claimed in claim 1, wherein a collimating lens is disposed between the emission filter of the filter wheel and the photodiode detection module.
6. The multi-channel switching device for Photodiode (PD) -based fluorescence detection as claimed in claim 1, wherein an excitation light aperture and a fluorescence signal aperture are provided above the filter wheel housing.
7. The multi-channel switching device for Photodiode (PD) -based fluorescence detection as claimed in claim 1, wherein a light guide element is disposed above the filter wheel housing.
8. The multi-channel switching device for Photodiode (PD) -based fluorescence detection according to claim 1, wherein the multi-channel switching device comprises a motor drive assembly coupled to a filter wheel.
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| CN202021641309.6U CN212748736U (en) | 2020-08-10 | 2020-08-10 | Multi-channel switching device for fluorescence detection based on Photodiode (PD) |
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| CN202021641309.6U CN212748736U (en) | 2020-08-10 | 2020-08-10 | Multi-channel switching device for fluorescence detection based on Photodiode (PD) |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113789261A (en) * | 2021-11-18 | 2021-12-14 | 深圳市刚竹智造科技有限公司 | Rotary wheel type optical assembly and rotary wheel type PCR optical system |
| CN115161187A (en) * | 2022-09-06 | 2022-10-11 | 鲲鹏基因（北京）科技有限责任公司 | Multichannel fluorescence detection device and PCR instrument |
-
2020
- 2020-08-10 CN CN202021641309.6U patent/CN212748736U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113789261A (en) * | 2021-11-18 | 2021-12-14 | 深圳市刚竹智造科技有限公司 | Rotary wheel type optical assembly and rotary wheel type PCR optical system |
| CN115161187A (en) * | 2022-09-06 | 2022-10-11 | 鲲鹏基因（北京）科技有限责任公司 | Multichannel fluorescence detection device and PCR instrument |
| CN115161187B (en) * | 2022-09-06 | 2023-04-07 | 鲲鹏基因（北京）科技有限责任公司 | Multi-channel fluorescence detection device and PCR instrument |
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