CN212713579U - Cell climbing device - Google Patents
Cell climbing device Download PDFInfo
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- CN212713579U CN212713579U CN202021378845.1U CN202021378845U CN212713579U CN 212713579 U CN212713579 U CN 212713579U CN 202021378845 U CN202021378845 U CN 202021378845U CN 212713579 U CN212713579 U CN 212713579U
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- climbing
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Abstract
The utility model relates to the technical field of biological experiment appliances, in particular to a cell climbing sheet device, which comprises a cell culture plate, wherein a plurality of climbing sheet mechanisms are arranged on the upper surface of the cell culture plate, each climbing sheet mechanism comprises a plurality of supports and climbing sheets, the supports are fixed on the cell culture plate, the climbing sheets are placed on the supports, and the edges of the climbing sheets are provided with bulges; the utility model discloses the climbing piece of equidimension not can be placed to the support that sets up, has improved the availability factor of device, and overall structure sets up simple, reasonable in design, easily change, has practical value.
Description
Technical Field
The utility model relates to a biological assay utensil technical field specifically is a cell climbing piece device.
Background
Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.
In the process of cell culture, at present, a slide is mostly placed in a culture dish/culture plate, before conventional cells are spread on the slide, when the cells are basically full on the slide, the slide is taken out, and after rinsing, residual culture medium and serum are sufficiently removed, then the cells are fixed, aired and stored in a container for standby. However, the slide is easy to slide and difficult to fix in the current culture method; cells can grow in the crack of the container of the slide glass, so that the cells grow on two sides, the growth area and the growth quantity of the cells are not controlled well in the test, and the identity of the test has errors.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to overcome the not enough of prior art, provide a cell slide device rational in infrastructure, easy to assemble and pick, can improve experiment uniformity.
The utility model provides a pair of cell climbing piece device, including the cell culture board, the upper surface of cell culture board is equipped with a plurality of climbing piece mechanism, climbing piece mechanism includes a plurality of support and climbing piece, the support is fixed on the cell culture board, the climbing piece is placed on the support, just the edge of climbing piece is equipped with the arch, sets up the arch and not only can make things convenient for snatching of climbing piece, also is favorable to preventing spilling, the energy saving of antibody.
Preferably, the climbing sheet is circular.
Preferably, the screenshot of the main view of the support is a right-angled triangle, so that a good fixing effect can be achieved.
Preferably, the number of the supports is 3, and the supports are uniformly arranged on the outer side of the climbing sheet along the circumference in a circular mode.
Preferably, the climbing piece is placed on the hypotenuse of support, and the circle that each support hypotenuse bottom encloses is less than the climbing piece, and the circle that each support hypotenuse top encloses is greater than the climbing piece, is convenient for place the climbing piece of equidimension not consequently.
Preferably, the cell culture plate is one of circular or rectangular.
Preferably, the number of the sheet climbing mechanisms is 6, the sheet climbing mechanisms are arranged in a pairwise symmetrical mode, and the experimental efficiency can be greatly improved due to the arrangement of the plurality of sheet climbing mechanisms.
Preferably, the slide is a standard glass slide with the thickness of 0.14-0.17mm, and the thickness of the bulge is 2-4 mm.
Preferably, the thickness of the climbing sheet is 0.15mm, and the thickness of the protrusion is 2 mm.
The utility model is provided with the bulge, which not only facilitates the grabbing of the climbing sheet, but also is beneficial to preventing the antibody from leaking, saves energy and improves the experimental identity; the utility model discloses the climbing piece of equidimension not can be placed to the support that sets up, has improved the availability factor of device, the utility model discloses an overall structure sets up simple, reasonable in design, easily change, has practical value.
Drawings
Fig. 1 is a schematic view of an overall three-dimensional structure in embodiment 1 of the present invention.
Fig. 2 is a schematic front view of the whole structure in embodiment 1 of the present invention.
Fig. 3 is a schematic top view of the whole structure according to embodiment 1 of the present invention.
Fig. 4 is a schematic perspective view of the slide-climbing mechanism of the present invention.
Fig. 5 is a schematic view of the three-dimensional structure of the bracket of the present invention.
In the figure: 1-cell culture plate, 2-slide climbing mechanism, 3-bracket, 4-slide climbing and 5-bulge.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Detailed description of the preferred embodiment 1
Fig. 1 to 5 are schematic structural views of a cell slide device of the present invention, from which it can be seen that the cell slide device of the present invention comprises a rectangular cell culture plate 1, 6 slide mechanisms 2 are arranged on the upper surface of the cell culture plate in a pairwise symmetrical manner, and the arrangement of a plurality of slide mechanisms can greatly improve the experimental efficiency; the climbing piece mechanism comprises 3 right-angled triangle supports 3 and a circular climbing piece 4, the supports are fixed on a cell culture plate, the climbing piece is placed on the oblique side of the right-angled triangle supports, the 3 supports are uniformly arranged on the outer side of the climbing piece along the circumference in an annular mode, the circle enclosed by the bottom of the oblique side of each support is smaller than the climbing piece, the circle enclosed by the top of the oblique side of each support is larger than the climbing piece, therefore, climbing pieces with different sizes can be placed conveniently, the supports with right-angled triangle structures can well fix the climbing piece, and the climbing piece is a standard glass sheet with the thickness of 0.15 mm; the edge of the climbing piece is provided with the bulge 5, the thickness of the bulge is 2mm, the climbing piece can be conveniently grabbed, the antibody can be prevented from leaking, and energy is saved.
Specific example 2
With reference to fig. 1 to 5 and embodiment 1, the present invention further provides a cell slide device, which includes a circular cell culture plate 1, wherein 6 slide mechanisms 2 are symmetrically arranged on the upper surface of the cell culture plate, and the arrangement of the plurality of slide mechanisms can greatly improve the experimental efficiency; the climbing piece mechanism comprises 3 right-angled triangle supports 3 and a circular climbing piece 4, the supports are fixed on a cell culture plate, the climbing piece is placed on the oblique side of the right-angled triangle supports, the 3 supports are uniformly arranged on the outer side of the climbing piece along the circumference in an annular mode, the circle enclosed by the bottom of the oblique side of each support is smaller than the climbing piece, the circle enclosed by the top of the oblique side of each support is larger than the climbing piece, therefore, climbing pieces with different sizes can be placed conveniently, the supports with right-angled triangle structures can well fix the climbing piece, and the climbing piece is a standard glass sheet with the thickness of 0.15 mm; the edge of the climbing piece is provided with the bulge 5, the thickness of the bulge is 2mm, the climbing piece can be conveniently grabbed, the antibody can be prevented from leaking, and energy is saved.
When the cell climbing sheet is used, firstly, the climbing sheet is pretreated, namely, high-temperature sterilization is carried out, the cell climbing sheet is soaked in alcohol for 1 hour, then, a support of a climbing sheet mechanism is fixed on a cell culture plate, then, cultured cells are added into the climbing sheet, after a preset state is reached, the climbing sheet is placed on the inclined edge of the support, due to the arrangement of the protrusions, the climbing sheet can be conveniently grabbed, the leakage of antibodies is prevented, energy is saved, after the cell climbing sheet grows for a certain time, the climbing sheet is taken down through the protrusions at the edge of the climbing sheet, the climbing sheet is treated, namely, the climbing sheet is washed for 3 times by PBS (phosphate buffer solution) and then is fixed in cold acetone for half an hour, after the cell climbing sheet is fully dried, the climbing sheet is transferred into a container, and is sealed by covering, and is kept for standby.
In summary, the protrusions are arranged in the above embodiments of the present invention, which not only facilitates the grabbing of the climbing sheet, but also facilitates the prevention of the antibody leakage, saves energy, and improves the experimental identity; the utility model discloses the climbing piece of equidimension not can be placed to the support that sets up, has improved the availability factor of device, and overall structure sets up simple, reasonable in design, easily change, has practical value.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can understand the changes or substitutions within the technical scope of the present invention, and the protection scope of the present invention should be covered within the protection scope of the present invention.
Claims (9)
1. A cell slide device, its characterized in that: including cell culture board (1), the upper surface of cell culture board is equipped with a plurality of climbing piece mechanism (2), climbing piece mechanism includes a plurality of support (3) and climbing piece (4), the support is fixed on the cell culture board, the climbing piece is placed on the support, just the edge of climbing piece is equipped with arch (5).
2. The cell slide apparatus according to claim 1, wherein: the climbing sheet is circular.
3. The cell slide apparatus according to claim 2, wherein: the main view screenshot of the support is a right triangle.
4. The cell slide apparatus according to claim 3, wherein: the support is equipped with 3, just the support evenly sets up in the outside of climbing the piece along circumference annular.
5. The cell slide apparatus according to claim 4, wherein: the climbing pieces are placed on the inclined edges of the supports, the circle enclosed by the bottoms of the inclined edges of the supports is smaller than the climbing pieces, and the circle enclosed by the tops of the inclined edges of the supports is larger than the climbing pieces.
6. The cell slide apparatus according to claim 1, wherein: the cell culture plate is one of circular or rectangular.
7. The cell slide apparatus according to claim 1, wherein: the number of the sheet climbing mechanisms is 6, and the sheet climbing mechanisms are arranged in a pairwise symmetrical mode.
8. The cell slide apparatus according to claim 1, wherein: the climbing sheet is a standard glass sheet with the thickness of 0.14-0.17mm, and the thickness of the bulge is 2-4 mm.
9. The cell slide apparatus according to claim 8, wherein: the thickness of climbing piece is 0.15mm, bellied thickness is 2 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021378845.1U CN212713579U (en) | 2020-07-14 | 2020-07-14 | Cell climbing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021378845.1U CN212713579U (en) | 2020-07-14 | 2020-07-14 | Cell climbing device |
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| Publication Number | Publication Date |
|---|---|
| CN212713579U true CN212713579U (en) | 2021-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021378845.1U Active CN212713579U (en) | 2020-07-14 | 2020-07-14 | Cell climbing device |
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| CN (1) | CN212713579U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023281951A1 (en) * | 2021-07-06 | 2023-01-12 | 株式会社日立ハイテク | Slide glass carrier |
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2020
- 2020-07-14 CN CN202021378845.1U patent/CN212713579U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023281951A1 (en) * | 2021-07-06 | 2023-01-12 | 株式会社日立ハイテク | Slide glass carrier |
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