CN212697391U

CN212697391U - Cell cryopreserving gradient cooling controlling means

Info

Publication number: CN212697391U
Application number: CN202021672459.3U
Authority: CN
Inventors: 章万古
Original assignee: Guanhao Precision Hangzhou Biotechnology Co ltd
Current assignee: Guanhao Precision Hangzhou Biotechnology Co ltd
Priority date: 2020-08-12
Filing date: 2020-08-12
Publication date: 2021-03-16
Anticipated expiration: 2030-08-12

Abstract

The utility model discloses a gradient cooling control device is deposited to cell freezing relates to cooling control field, which comprises an outer shell, be equipped with the inner bag in the shell, first circular through-hole has all been seted up to the bottom side of shell and inner bag, and same center pin is installed to two first circular through-hole internal rotations, the top fixedly connected with base of center pin, the bottom fixedly connected with gear wheel of center pin, one side fixed mounting of shell has spacing diaphragm. The utility model discloses it is rational in infrastructure, place the cell that needs the cooling in the culture solution, sealed back is cold-stored thirty minutes back in the refrigerator, take out and place on the base, the transmission shaft drives adjusting gear and rotates, adjusting gear drives the tooth cover and removes, the tooth cover drives the main adjusting rod and removes, it drives first regulation pin and second regulation pin removal simultaneously to adjust the pole, and then realize opening one-way regulating valve and relief valve simultaneously, prevent that gas nitrogen from leading to atmospheric pressure to rise, lead to the cell impaired.

Description

Cell cryopreserving gradient cooling controlling means

Technical Field

The utility model relates to a cooling control field, in particular to cell cryopreserving gradient cooling control device.

Background

In cell experiments, cell cryopreservation is a very critical step, and the quality of cell cryopreservation directly influences the subsequent cell recovery survival rate. Besides the requirement of appropriate solution (i.e. cell culture solution, fetal bovine serum (10-30%) with appropriate concentration) for cell cryopreservation, the cell cooling rate is also very critical.

The cell is when freezing, most taboo rapid cooling, rapid cooling can cause irreversible damage to the cell to reduce the survival rate of cell after the recovery, but among the prior art, for making the cell can slow cooling, avoid the injury that rapid cooling caused the cell, need the experimenter manually operation, a whole set of cooling operation gets down and wastes time and energy, and can not guarantee that the temperature that the cell in the culture solution receives is even, consequently urgently need one kind can assist the supplementary heat sink of experimenter operation, thereby improves the survival rate of cell.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell cryopreserving gradient cooling control device to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a gradient cooling control device for cell cryopreservation comprises a shell, wherein an inner container is arranged in the shell, first circular through holes are formed in the bottom sides of the shell and the inner container, the two first circular through holes are rotatably provided with the same central shaft, the top end of the central shaft is fixedly connected with a base, the bottom end of the central shaft is fixedly connected with a big gear, a limit transverse plate is fixedly arranged at one side of the shell, a second round through hole is arranged on the limit transverse plate, a rotating shaft is rotatably arranged in the second round through hole, the top end of the rotating shaft is fixedly connected with an adjusting disc, a short rod is eccentrically and fixedly arranged on the top side of the adjusting disc, a pinion is fixedly sleeved at the bottom end of the rotating shaft, the small gear is meshed with the large gear, a sealing cover is rotatably installed on one side of the shell, and a temperature display screen is fixedly installed on one side of the shell.

Preferably, a gas nitrogen inlet is formed in one side of the outer shell and one side of the inner container, an annular pipe is fixedly mounted on one side of the gas nitrogen inlet, a plurality of gas nitrogen nozzles are fixedly connected to the bottom side of the annular pipe, a one-way regulating valve is fixedly mounted on the other side of the gas nitrogen inlet, a second circular through hole is formed in a regulating pipe of the one-way regulating valve, a first regulating thin rod is arranged in the second circular through hole, and one end of the first regulating thin rod penetrates through the second circular through hole and is rotatably mounted with a main regulating rod.

Preferably, a gas nitrogen outlet is formed in one side of the outer shell and one side of the inner container, a pressure release valve is fixedly mounted at the gas nitrogen outlet, a third circular hole is formed in an adjusting pipe of the pressure release valve, a second adjusting thin rod is arranged on the third circular hole, and one end of the second adjusting thin rod penetrates through the third circular hole and is rotatably mounted on one side of the main adjusting rod.

Preferably, a gear sleeve is fixedly sleeved on the main adjusting rod.

Preferably, a transmission shaft is rotatably mounted on one side of the shell, an adjusting gear is fixedly sleeved on the transmission shaft, a handle is fixedly connected to one end of the transmission shaft, and the adjusting gear is meshed with the gear sleeve.

Preferably, two limiting plates are fixedly mounted on one side of the shell, fourth circular through holes are formed in the two limiting plates, and the main adjusting rod penetrates through the two fourth circular through holes in sequence.

Preferably, a fifth circular through hole and a sixth circular through hole are formed in the shell and the inner container, and a liquid nitrogen inlet pipe and a liquid nitrogen outlet pipe are fixedly mounted on the fifth circular through hole and the sixth circular through hole respectively.

The utility model discloses a technological effect and advantage:

1. the utility model has reasonable structure, cells needing cooling are placed in the culture solution, after being refrigerated in a refrigerator for thirty minutes after being sealed, the cells are taken out and placed on the base, the sealing cover is covered, the handle is moved at the moment, the handle drives the transmission shaft to rotate, the transmission shaft drives the adjusting gear to rotate, the adjusting gear drives the gear sleeve to move, the gear sleeve drives the main adjusting rod to move, the adjusting rod simultaneously drives the first adjusting thin rod and the second adjusting thin rod to move, and then the one-way adjusting valve and the pressure relief valve are opened simultaneously, so that the air nitrogen is prevented from causing the air pressure to rise, and the cells are damaged;

2. through the design of adjustment disk, gaseous nitrogen passes through gaseous nitrogen nozzle and gets into the inner bag, rotates the adjustment disk this moment, and the adjustment disk drives pinion rotation, and the pinion drives the gear wheel and rotates, and the gear wheel drives the center pin and rotates, and the center pin drives the base and rotates, thereby realizes slowly rotating the cultured cell, and it is even to realize catching cold, thereby improves the survival rate of cell, lets in gaseous nitrogen thirty minutes after and lets in the liquid nitrogen through the liquid nitrogen admission pipe, and then falls to minimum temperature, whole design simple structure, easy operation.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

fig. 2 is a schematic perspective view of another perspective of the present invention;

FIG. 3 is an enlarged schematic view of the structure at A of FIG. 1 according to the present invention;

fig. 4 is a schematic view of the top view structure of the present invention.

In the figure: 1. a housing; 2. an inner container; 3. a bull gear; 4. a transverse limiting plate; 5. a pinion gear; 6. an adjusting disk; 7. a base; 8. a one-way regulating valve; 9. a gas nitrogen nozzle; 10. a first adjustment pin; 11. a limiting plate; 12. a main adjustment lever; 13. a gear sleeve; 14. a second adjusting slender rod; 15. a pressure relief valve; 16. a drive shaft; 17. an adjusting gear; 18. a handle; 19. introducing liquid nitrogen into the pipe; 20. a liquid nitrogen outlet pipe; 21. a sealing cover; 22. and a temperature display screen.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model provides a cell freezing gradient cooling control device as shown in figures 1-4, which comprises a shell 1, an inner container 2 is arranged in the shell 1, first circular through holes are respectively arranged at the bottom sides of the shell 1 and the inner container 2, the same central shaft is rotationally arranged in the two first circular through holes, the top end of the central shaft is fixedly connected with a base 7, the bottom end of the central shaft is fixedly connected with a big gear 3, a spacing transverse plate 4 is fixedly arranged at one side of the shell 1, a second circular through hole is arranged on the spacing transverse plate 4, a rotational shaft is rotationally arranged in the second circular through hole, the top end of the rotational shaft is fixedly connected with an adjusting disc 6, a short rod is eccentrically and fixedly arranged at the top side of the adjusting disc 6, a small gear 5 is fixedly sleeved at the bottom end of the rotational shaft, the small gear 5 is meshed with the big gear 3, a, the temperature display screen 22 can directly penetrate the internal temperature of the inner container 2.

Further, in above-mentioned scheme, the gas nitrogen entry has all been seted up to one side of shell 1 and inner bag 2, one side fixed mounting of gas nitrogen entry has the ring pipe, a plurality of gas nitrogen nozzles of bottom side fixedly connected with 9 of ring pipe, the opposite side fixed mounting of gas nitrogen entry has one-way regulating valve 8, the circular through-hole of second has been seted up on one-way regulating valve 8's the control tube, be equipped with first regulation pin 10 in the circular through-hole of second, the one end of first regulation pin 10 runs through the circular through-hole of second and rotates and install main regulation pole 12, design through one-way regulating valve 8, the control to gas nitrogen has been realized.

Further, in the above scheme, the gas nitrogen outlet has all been seted up to one side of shell 1 and inner bag 2, and gas nitrogen outlet fixed mounting has pressure release valve 15, has seted up the third circular hole on pressure release valve 15's the control tube, is equipped with the second on the third circular through-hole and adjusts thin pole 14, and the one end of second regulation thin pole 14 runs through the third circular through-hole and rotates and install in one side of main regulating rod 12, through the design of pressure release valve 15, prevents that internal pressure is too big.

Further, in the above scheme, the main adjusting rod 12 is fixedly sleeved with the gear sleeve 13, and the transmission of mechanical energy is realized through the design of the gear sleeve 13.

Further, in the above scheme, a transmission shaft 16 is rotatably mounted on one side of the housing 1, an adjusting gear 17 is fixedly sleeved on the transmission shaft 16, a handle 18 is fixedly connected to one end of the transmission shaft 16, the adjusting gear 17 is engaged with the gear sleeve 13, and the transmission of mechanical energy is realized through the design of the adjusting gear 17.

Further, in above-mentioned scheme, one side fixed mounting of shell 1 has two limiting plates 11, has all seted up the circular through-hole of fourth on two limiting plates 11, and main regulating rod 12 runs through two circular through-holes of fourth in proper order, through the design of two limiting plates 11, has realized limit function.

Further, in the above scheme, a fifth circular through hole and a sixth circular through hole are both formed in the shell 1 and the inner container 2, and a liquid nitrogen inlet pipe 19 and a liquid nitrogen outlet pipe 20 are respectively and fixedly mounted on the fifth circular through hole and the sixth circular through hole, and are designed through the liquid nitrogen outlet pipe 20. The function of circulating liquid nitrogen is realized.

This practical theory of operation: placing cells needing cooling in a culture solution, refrigerating for thirty minutes in a refrigerator after sealing, taking out and placing the cells on a base 7, covering a sealing cover 21, moving a handle 18 at the moment, driving a transmission shaft 16 to rotate by the handle 18, driving an adjusting gear 17 to rotate by the transmission shaft 16, driving a gear sleeve 13 to move by the adjusting gear 17, driving a main adjusting rod 12 to move by the gear sleeve 13, driving a first adjusting thin rod 10 and a second adjusting thin rod 14 to move by the main adjusting rod 12 simultaneously, further realizing the simultaneous opening of a one-way adjusting valve 8 and a pressure release valve 15, preventing gas nitrogen from causing air pressure to rise and causing cell damage, allowing the gas nitrogen to enter an inner container 2 through a gas nitrogen nozzle 9, rotating an adjusting disc 6 at the moment, driving a small gear 5 to rotate by the adjusting disc 6, driving a large gear 3 to rotate by the small gear 5, driving a large gear 3 to rotate a central shaft, driving the base, realize the even quilt cold to improve the survival rate of cell, let in gas nitrogen thirty minutes after, let in the liquid nitrogen through liquid nitrogen admission pipe 19, and then fall to minimum temperature, whole design simple structure, the processing ease.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. The utility model provides a gradient cooling controlling means is deposited to cell freezing, includes shell (1), its characterized in that: the inner container (2) is arranged in the shell (1), first circular through holes are formed in the bottom sides of the shell (1) and the inner container (2), the two first circular through holes are internally rotatably provided with the same central shaft, the top end of the central shaft is fixedly connected with the base (7), the bottom end of the central shaft is fixedly connected with the gear wheel (3), one side of the shell (1) is fixedly provided with a limiting transverse plate (4), a second circular through hole is formed in the limiting transverse plate (4), a rotating shaft is rotatably arranged in the second circular through hole, the top end of the rotating shaft is fixedly connected with an adjusting disc (6), a short rod is eccentrically and fixedly arranged on the top side of the adjusting disc (6), a small gear (5) is fixedly sleeved at the bottom end of the rotating shaft, the small gear (5) is meshed with the gear wheel (3), and a sealing cover (21) is, and a temperature display screen (22) is fixedly arranged on one side of the shell (1).

2. The gradient cooling control device for cell cryopreservation according to claim 1, wherein: the gas nitrogen inlet has all been seted up to one side of shell (1) and inner bag (2), one side fixed mounting of gas nitrogen inlet has the ring pipe, and the bottom side fixedly connected with of ring pipe has a plurality of gas nitrogen nozzles (9), the opposite side fixed mounting of gas nitrogen inlet has one-way control valve (8), the circular through-hole of second has been seted up on the control tube of one-way control valve (8), is equipped with first regulation pin (10) in the circular through-hole of second, the one end of first regulation pin (10) runs through the circular through-hole of second and rotates and installs main regulation pole (12).

3. The gradient cooling control device for cell cryopreservation according to claim 1, wherein: the gas nitrogen outlet has all been seted up to one side of shell (1) and inner bag (2), gas nitrogen outlet department fixed mounting has relief valve (15), the third circular hole has been seted up on the control tube of relief valve (15), be equipped with second regulation pin (14) on the third circular through-hole, the one end of second regulation pin (14) runs through third circular through-hole and rotates and install in one side of main regulating rod (12).

4. The gradient cooling control device for cell cryopreservation according to claim 2, wherein: the main adjusting rod (12) is fixedly sleeved with a gear sleeve (13).

5. The gradient cooling control device for cell cryopreservation according to claim 1, wherein: one side of shell (1) is rotated and is installed transmission shaft (16), fixed cover on transmission shaft (16) has connect adjusting gear (17), the one end fixedly connected with handle (18) of transmission shaft (16), adjusting gear (17) and tooth cover (13) mesh mutually.

6. The gradient cooling control device for cell cryopreservation according to claim 1, wherein: one side fixed mounting of shell (1) has two limiting plates (11), the circular through-hole of fourth has all been seted up on two limiting plates (11), and main regulating stem (12) runs through two circular through-holes of fourth in proper order.

7. The gradient cooling control device for cell cryopreservation according to claim 1, wherein: a fifth circular through hole and a sixth circular through hole are formed in the shell (1) and the inner container (2), and a liquid nitrogen inlet pipe (19) and a liquid nitrogen outlet pipe (20) are fixedly mounted on the fifth circular through hole and the sixth circular through hole respectively.