CN212260068U - Device for keeping high activity of in-vitro pollen of gramineous crops - Google Patents

Device for keeping high activity of in-vitro pollen of gramineous crops Download PDF

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Publication number
CN212260068U
CN212260068U CN202020551253.9U CN202020551253U CN212260068U CN 212260068 U CN212260068 U CN 212260068U CN 202020551253 U CN202020551253 U CN 202020551253U CN 212260068 U CN212260068 U CN 212260068U
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pollen
dry ice
isolated
maintaining high
chamber
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CN202020551253.9U
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Chinese (zh)
Inventor
张从合
王慧
冯冲
张道林
严志
方玉
周桂香
申广勒
张云虎
陈琳
李方宝
庞战士
王林
刘兴江
汪和廷
杨韦
杨力
黄艳玲
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Jiang Jiayue
Anhui Win All Hi Tech Seed Co ltd
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Jiang Jiayue
Anhui Win All Hi Tech Seed Co ltd
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Abstract

The utility model relates to a keep high active device of gramineous crop separation pollen, gather the room and locate glass bead locker room, drier locker room and the suction fan of pollen collection room upper end including the pollen, the lower extreme of pollen collection room is connected with inhales the powder ware, it is connected with dry ice ration sprayer on the powder ware to inhale. This keep device of gramineae crop separation pollen high activity can be when collecting pollen with kibbling dry ice and the air ration mixture that contains the pollen, reaches the effect of rapid sublimation cooling, makes pollen collection room temperature control all the timeAt 7-15 deg.C, the refrigeration is rapid and uniform, the pollen collection time is prolonged, the high activity of the collected pollen is maintained, and CO is passed through2The method has the advantages of inhibiting the respiration of the pollen, reducing the consumption of nutrient substances for storing the pollen, being beneficial to keeping the activity of the pollen, greatly improving the pollen collection quality and finally improving the pollen setting rate.

Description

Device for keeping high activity of in-vitro pollen of gramineous crops
Technical Field
The utility model belongs to the technical field of pollen is gathered, concretely relates to keep device of gramineae crop separation pollen high vitality.
Background
Two varieties with different genetic properties are hybridized, the first filial generation shows very strong fertility, vitality, stress resistance and adaptability, and has more advantages than parents in the aspects of yield, quality and the like; the pollen plays an important role in sexual propagation of plants, and male parent genetic information transmitted after the pollen germinates is the key for seed formation; the collection and storage of pollen and the pollen activity are the foundation of seed reproduction and production and are also important links in the breeding of male sterile lines and the hybrid seed production; before carrying out crop hybrid seed production, a large amount of pollen of male parent materials needs to be collected so as to facilitate hybridization; in order to meet the centralized requirement of large-area female parent pollination in sterile lines of different crops, male parent pollen needs to be collected in advance; how to collect pollen and keep the activity of the pollen is the key of hybrid seed production work.
At present, pollen collection is mainly completed manually and mechanically, the manual pollen collection method has the disadvantages of high labor intensity, low efficiency and uneven pollen collection, and the pollen activity can be reduced faster by manual operation, so that the seed production yield is reduced; in the current mechanical pollen collection process, due to the fact that the temperature is difficult to reduce, the collection time is long, and other conditions are not properly controlled, pollen activity is usually obviously reduced, and the pollen fertilization and fructification capability is seriously influenced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the problems and provide a device which has simple structure and reasonable design and can keep the high activity of the isolated pollen of the gramineous crops.
The utility model discloses a following technical scheme realizes above-mentioned purpose:
the utility model provides a keep gramineae crop separation pollen high-activity device, includes pollen collection room and locates glass bead locker room, drier locker room and the suction fan of pollen collection room upper end, the lower extreme of pollen collection room is connected with inhales the powder ware, it is connected with dry ice ration sprayer on the powder ware to inhale.
As the utility model discloses a further optimization scheme, inhale whitewashed ware and include the casing, connect pollen conveyer pipe in the casing upper end, locate second filter screen on the shells inner wall, connect connecting plate, swing joint of casing one end on the connecting plate rotary rod and connect the powder driving operation pole at the rotary rod lower extreme.
As the utility model discloses a further optimization scheme, dry ice ration spraying apparatus includes the dry ice storage box, connects at the electric putter of dry ice storage box one end, connects the piston on the electric putter output and connects the dry ice spray tube at the dry ice storage box other end, the dry ice spray tube is connected on the pollen conveyer pipe, and is equipped with the third solenoid valve on the dry ice spray tube, be detachable connection between the upper end of pollen conveyer pipe and the pollen collection room.
As a further optimization scheme of the utility model, the indoor a plurality of pollen box that is equipped with is gathered to the pollen, and the indoor conveyer pipe that is equipped with of pollen collection, glass bead locker room and drier locker room all with duct connection.
As the utility model discloses a further optimization scheme, the junction of glass bead locker room and conveyer pipe is equipped with first solenoid valve, the drier locker room is equipped with the second solenoid valve with the junction of conveyer pipe, the conveyer pipe all communicates with a plurality of pollen box.
As a further optimization scheme of the utility model, be equipped with temperature and humidity sensor on the upper end inner wall of room is gathered to pollen, the position that is close to the suction fan on the inner wall of room is gathered to pollen is equipped with first filter screen, first filter screen is for melting nonwoven fabric or microfiber filter screen, and the net gape aperture is less than 10 μm.
As a further optimization scheme of the utility model, the upper end of rotary rod is connected with the stopper, and is connected with the holding rod on the rotary rod, the second filter screen is plastic net or non-woven fabrics net, and the net gape aperture is 60-300 mu m.
As a further optimization scheme of the utility model, be equipped with the dry ice particle in the dry ice storage box, the particle diameter of dry ice particle is less than 2mm, is equipped with the glass microballon in the glass microballon locker room, be equipped with the drier in the drier locker room, the drier is silica gel.
As a further optimization scheme of the utility model, the distance between the powder driving operating rod and the inlet at the lower end of the shell is 20-30 cm.
The beneficial effects of the utility model reside in that:
1) the utility model discloses can be when collecting pollen with kibbling dry ice and the air ration mixture that contains pollen, reach the effect of rapid sublimation cooling, make pollen collection room temperature control all the time at 7-15 ℃, the refrigeration is rapid and even, prolonged the acquisition time of pollen, kept the high vitality of the pollen of gathering simultaneously through CO2The respiration of the pollen is inhibited, the consumption of nutrient substances for storing the pollen is reduced, the vitality of the pollen is kept, the pollen collection quality is greatly improved, and the pollen setting rate is finally improved;
2) the utility model discloses simple structure, stability is high, reasonable in design, the realization of being convenient for.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a view showing the powder sucking device and the dry ice quantitative spraying device of the present invention;
fig. 3 is a schematic structural diagram of the fan of the present invention.
In the figure: 1. a pollen collection chamber; 101. a pollen box; 102. a first filter screen; 103. a delivery pipe; 2. a glass bead storage chamber; 201. a first solenoid valve; 3. a desiccant storage compartment; 301. a second solenoid valve; 4. a suction fan; 5. a temperature and humidity sensor; 6. a dry ice quantitative sprayer; 601. an electric push rod; 602. a piston; 603. a dry ice storage bin; 604. a dry ice spray tube; 605. a third electromagnetic valve; 7. a powder suction device; 701. a housing; 702. a second filter screen; 703. a pollen conveying pipe; 704. a connecting plate; 705. a holding rod; 706. rotating the rod; 707. and a powder driving operating rod.
Detailed Description
The present application will now be described in further detail with reference to the drawings, it should be noted that the following detailed description is given for illustrative purposes only and is not to be construed as limiting the scope of the present application, as those skilled in the art will be able to make numerous insubstantial modifications and adaptations to the present application based on the above disclosure.
Example 1
As shown in fig. 1-3, a device for maintaining high activity of in vitro pollen of gramineous crops comprises a pollen collecting chamber 1, a glass bead storage chamber 2, a desiccant storage chamber 3 and a suction fan 4, wherein the glass bead storage chamber 2, the desiccant storage chamber 3 and the suction fan 4 are arranged at the upper end of the pollen collecting chamber 1, the lower end of the pollen collecting chamber 1 is connected with a powder suction device 7, and the powder suction device 7 is connected with a dry ice quantitative sprayer 6;
the powder sucker 7 comprises a shell 701, a pollen conveying pipe 703 connected to the upper end of the shell 701, a second filter screen 702 arranged on the inner wall of the shell 701, a connecting plate 704 connected to one end of the shell 701, a rotating rod 706 movably connected to the connecting plate 704 and a powder driving operating rod 707 connected to the lower end of the rotating rod 706;
the dry ice quantitative sprayer 6 comprises a dry ice storage box 603, an electric push rod 601 connected with one end of the dry ice storage box 603, a piston 602 connected with the output end of the electric push rod 601, and a dry ice spraying pipe 604 connected with the other end of the dry ice storage box 603, wherein the dry ice spraying pipe 604 is connected with a pollen conveying pipe 703, a third electromagnetic valve 605 is arranged on the dry ice spraying pipe 604, and the upper end of the pollen conveying pipe 703 is detachably connected with the pollen collecting chamber 1; the dry ice storage box 603 is internally provided with dry ice particles, the particle size of the dry ice particles is smaller than 2mm, the dry ice quantitative sprayer 6 can push the piston 602 to convey dry ice through the electric push rod 601 according to the field temperature and the air inlet flow of collected pollen, the dry ice spraying speed can be regulated and controlled through the pushing speed and the third electromagnetic valve 605 when the dry ice is conveyed, and the lower the field temperature is, the slower the dry ice spraying speed is; temperature of fieldThe higher the degree is, the higher the spraying speed of the dry ice is, so that the collection chamber can quantitatively mix gaseous CO2The temperature is kept between 7 and 15 ℃ all the time, the temperature and the water content of the pollen in vitro are reduced by rapid sublimation and a large amount of heat absorption and temperature reduction, and the high activity of the pollen is kept; at the same time due to the mixed CO2The respiration of the pollen can be inhibited, the consumption of nutrient substances for storing the pollen is reduced, and the pollen vitality is kept;
a plurality of pollen boxes 101 are arranged in the pollen collecting chamber 1, the pollen boxes 101 are used for storing collected pollen, a conveying pipe 103 is arranged in the pollen collecting chamber 1, the glass bead storage chamber 2 and the desiccant storage chamber 3 are both connected with the conveying pipe 103, and the conveying pipe 103 can convey objects stored in the glass bead storage chamber 2 and the desiccant storage chamber 3 to the pollen boxes 101;
a first electromagnetic valve 201 is arranged at the joint of the glass bead storage chamber 2 and the conveying pipe 103, a second electromagnetic valve 301 is arranged at the joint of the desiccant storage chamber 3 and the conveying pipe 103, the conveying pipe 103 is communicated with the plurality of pollen boxes 101, glass beads are arranged in the glass bead storage chamber 2, a desiccant is arranged in the desiccant storage chamber, the desiccant is silica gel, the first electromagnetic valve 201 can be regulated and controlled to quantitatively spray the glass beads to the pollen boxes 101 according to the collected pollen amount so as to prevent the collected pollen from agglomerating and keep the high activity of the pollen, meanwhile, the second electromagnetic valve 301 can be regulated and controlled to quantitatively spray the desiccant to the pollen boxes 101 according to the collected pollen amount so as to keep the humidity in the pollen collection chamber 1 at 10% -20% all the time and keep the high activity of the pollen;
a temperature and humidity sensor 5 is arranged on the inner wall of the upper end of the pollen collecting chamber 1, so that the temperature and the humidity in the pollen collecting chamber 1 can be monitored at any time;
a first filter screen 102 is arranged on the inner wall of the pollen collecting chamber 1 and close to the suction fan 4, the first filter screen 102 is a melt-blown non-woven fabric or microfiber filter screen, the aperture of the mesh opening is less than 10 μm, and the first filter screen is preferably in a honeycomb or folding shape, so that the dissipation of pollen can be prevented;
the second filter screen 702 is a plastic net or a non-woven net, and the aperture of the net opening is 60-300 μm so as to remove impurities such as anther, insects, broken leaves and the like;
the upper end of the rotating rod 706 is connected with a limiting block, and the rotating rod 706 is connected with a holding rod 705; the distance between the powder driving operating rod 707 and the inlet at the lower end of the shell 701 is 20-30cm, the powder driving operating rod 707 can be driven by the holding rod 705 to shake off pollen from the ear head and disperse the pollen above the ear head in a centralized manner, so that the powder absorber 7 can absorb a large amount of powder in a centralized manner, and the efficiency of collecting the pollen is obviously improved.
It should be noted that, in the collection process, the suction fan 4 starts to generate air flow and sucks the pollen from the pollen sucking device 7, the dry ice is conveyed into the pollen conveying pipe 703 through the dry ice quantitative sprayer 6 in the pollen sucking process, so that the pollen and dry ice mixture enters the pollen collection chamber 1, then the glass microspheres and the drying agent are controlled and conveyed according to the amount of the pollen, the drying degree in the pollen collection chamber 1 is controlled while the pollen is prevented from agglomerating, the temperature of the pollen collection chamber 1 can be controlled to be 7-15 ℃ all the time while the dry ice achieves the effect of rapid sublimation and temperature reduction, the refrigeration effect is rapid and uniform, the pollen collection time is prolonged, the high activity of the collected pollen is maintained, and meanwhile, the CO is used for absorbing the pollen2The method has the advantages of inhibiting the respiration of the pollen, reducing the consumption of nutrient substances for storing the pollen, being beneficial to keeping the activity of the pollen, greatly improving the pollen collection quality and finally improving the pollen setting rate.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Claims (9)

1. The utility model provides a keep gramineae crop separation pollen high-activity device, includes pollen collection chamber (1) and locates glass bead locker room (2), drier locker room (3) and suction fan (4) of pollen collection chamber (1) upper end, the lower extreme of pollen collection chamber (1) is connected with inhales whitewashed ware (7), its characterized in that: the powder suction device (7) is connected with a dry ice quantitative spraying device (6).
2. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 1, wherein: the powder suction device (7) comprises a shell (701), a pollen conveying pipe (703) connected to the upper end of the shell (701), a second filter screen (702) arranged on the inner wall of the shell (701), a connecting plate (704) connected to one end of the shell (701), a rotating rod (706) movably connected to the connecting plate (704) and a powder driving operating rod (707) connected to the lower end of the rotating rod (706).
3. The device for maintaining high activity of pollen isolated from gramineous crops as claimed in claim 2, wherein: the dry ice quantitative sprayer (6) comprises a dry ice storage box (603), an electric push rod (601) connected to one end of the dry ice storage box (603), a piston (602) connected to the output end of the electric push rod (601), and a dry ice spraying pipe (604) connected to the other end of the dry ice storage box (603), wherein the dry ice spraying pipe (604) is connected to a pollen conveying pipe (703), a third electromagnetic valve (605) is arranged on the dry ice spraying pipe (604), and the upper end of the pollen conveying pipe (703) is detachably connected with a pollen collecting chamber (1).
4. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 1, wherein: the pollen collecting chamber is characterized in that a plurality of pollen boxes (101) are arranged in the pollen collecting chamber (1), conveying pipes (103) are arranged in the pollen collecting chamber (1), and the glass bead storage chamber (2) and the drying agent storage chamber (3) are connected with the conveying pipes (103).
5. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 4, wherein: the junction of glass bead locker room (2) and conveyer pipe (103) is equipped with first solenoid valve (201), the junction of drier locker room (3) and conveyer pipe (103) is equipped with second solenoid valve (301), conveyer pipe (103) all communicate with a plurality of pollen box (101).
6. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 1, wherein: the pollen collecting device is characterized in that a temperature and humidity sensor (5) is arranged on the inner wall of the upper end of the pollen collecting chamber (1), a first filter screen (102) is arranged at a position, close to the suction fan (4), on the inner wall of the pollen collecting chamber (1), the first filter screen (102) is a melt-blown non-woven fabric or a microfiber filter screen, and the aperture of a screen opening is smaller than 10 microns.
7. The device for maintaining high activity of pollen isolated from gramineous crops as claimed in claim 2, wherein: the upper end of the rotating rod (706) is connected with a limiting block, the rotating rod (706) is connected with a holding rod (705), the second filter screen (702) is a plastic net or a non-woven fabric net, and the aperture of the net opening is 60-300 mu m.
8. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 3, wherein: the dry ice storage box (603) is internally provided with dry ice particles, the particle size of the dry ice particles is smaller than 2mm, glass beads are arranged in the glass bead storage chamber (2), and a drying agent is arranged in the drying agent storage chamber and is silica gel.
9. The device for maintaining high activity of pollen isolated from gramineous crops as claimed in claim 2, wherein: the distance between the powder removing operating rod (707) and the inlet at the lower end of the shell (701) is 20-30 cm.
CN202020551253.9U 2020-04-15 2020-04-15 Device for keeping high activity of in-vitro pollen of gramineous crops Active CN212260068U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202020551253.9U CN212260068U (en) 2020-04-15 2020-04-15 Device for keeping high activity of in-vitro pollen of gramineous crops

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202020551253.9U CN212260068U (en) 2020-04-15 2020-04-15 Device for keeping high activity of in-vitro pollen of gramineous crops

Publications (1)

Publication Number Publication Date
CN212260068U true CN212260068U (en) 2021-01-01

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ID=73876378

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202020551253.9U Active CN212260068U (en) 2020-04-15 2020-04-15 Device for keeping high activity of in-vitro pollen of gramineous crops

Country Status (1)

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CN (1) CN212260068U (en)

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