CN212128187U - Nicotinamide mononucleotide production system - Google Patents
Nicotinamide mononucleotide production system Download PDFInfo
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- CN212128187U CN212128187U CN202020444555.6U CN202020444555U CN212128187U CN 212128187 U CN212128187 U CN 212128187U CN 202020444555 U CN202020444555 U CN 202020444555U CN 212128187 U CN212128187 U CN 212128187U
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Abstract
The utility model discloses a nicotinamide mononucleotide production system, it has set up frequency conversion oscillation device in nicotinamide mononucleotide's production container, vibrates the subassembly through the frequency conversion to be less than 90 KHz's frequency, carry out the frequency conversion to the zymotic fluid and vibrate, with zymotic fluid homodisperse. The fermentation liquor after being uniformly dispersed is conveyed to a production container for producing nicotinamide mononucleotide through an airflow condenser at the top of the liquor container, so that the fermentation liquor can be uniformly mixed.
Description
Technical Field
The utility model relates to a cultivate nicotinamide mononucleotide technical field, especially relate to a nicotinamide mononucleotide production system.
Background
Currently, the cultivation of nicotinamide mononucleotide is divided into three methods: liquid fermentation, chemical synthesis and enzymatic methods. The liquid fermentation method is to use fermentation tank to carry out liquid fermentation of strain to collect thallus, because the physiological and biochemical metabolic pathways of the thallus produced by fermentation are different from those of nicotinamide mononucleotide, the biochemical synthesis capacity of the thallus is reduced, and high-purity nicotinamide mononucleotide cannot be obtained, so the components shared by nicotinamide mononucleotide are far away. The chemical synthesis method can shorten the fermentation time for the production of nicotinamide mononucleotide, but the synthesis route is long, the content of isomers and impurities is high, and more separation and purification steps are needed at the rear end to realize high yield. The enzyme method uses monomer for enzyme conversion, although it has high specificity, it is difficult to control the enzyme type, activity and reaction environment, and the production cost is increased.
The liquid fermentation method is to produce nicotinamide mononucleotide by inoculating culture liquid to transfer microbe producing medium, and has the advantages of obtaining the same component as that of organism, especially unique nicotinamide mononucleotide, replacing chemical synthesis and enzyme process, and avoiding damage of nicotinamide mononucleotide structure and lowered yield caused by over concentration and purification of nicotinamide mononucleotide. However, the disadvantages of liquid fermentation include more production equipment, more energy consumption and high production cost, so that equipment capable of meeting the requirements of liquid fermentation production environment becomes an important issue.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects in the technology, the utility model provides a nicotinamide mononucleotide production system.
The utility model provides a technical scheme that its technical problem adopted is:
a nicotinamide mononucleotide production system, comprising: a production vessel capable of producing nicotinamide mononucleotide by a microbial enzymatic process therein; a liquid container arranged in the production container for containing fermentation liquid, wherein the fermentation liquid is used for producing nicotinamide mononucleotide; the variable frequency oscillation assembly is arranged at the bottom of the liquid container and generates variable frequency oscillation to uniformly disperse the fermentation liquid; and the airflow condenser is arranged at the top of the liquid containing vessel, discharges the uniformly dispersed fermentation liquid out of the liquid containing vessel and conveys the fermentation liquid into the production container.
Preferably, the device also comprises a liquid preparation vessel for preparing the fermentation liquid, and the liquid preparation vessel is connected with the liquid containing vessel through an infusion passage.
Preferably, the liquid feeding passage is provided with a valve for opening or closing the liquid feeding passage to control the flow of the fermentation liquid between the liquid preparation vessel and the liquid containing vessel.
Preferably, the liquid supply path is connected to an upper opening of the side wall of the liquid container and to a lower opening of the side wall of the liquid dispensing container.
Preferably, the level of the lower opening of the side wall of the liquid distribution vessel is higher than the level of the upper opening of the side wall of the liquid containing vessel.
Preferably, the horizontal height of the lower opening of the side wall of the liquid preparation vessel is lower than the horizontal height of the upper opening of the side wall of the liquid containing vessel; wherein, the transfusion passage is provided with a transfusion pump to convey the fermentation liquid from the bottom of the liquid preparation vessel to the liquid containing vessel through the transfusion passage.
Preferably, the bottom of the liquid preparation vessel is provided with a stirring component; the stirring assembly consists of at least one stirrer.
Preferably, the agitator comprises a rotating shaft; the first frame body and the second frame body are fixed on the shaft body and basically combined into a rectangular frame; the first stirring plate and the second stirring plate are fixed on the shaft body and positioned in the rectangular frame; the third stirring plate is positioned in the rectangular frame and fixed on the first frame body; and the fourth stirring plate is positioned in the rectangular frame and is fixed on the second frame body.
Preferably, the first stirring plate, the second stirring plate, the third stirring plate and the fourth stirring plate are all limited to be arranged towards the shaft body; the first stirring plate, the second stirring plate, the third stirring plate and the fourth stirring plate are arranged on two sides of the shaft body in a centrosymmetric manner;
wherein, first stirring board and fourth stirring board all configure into and have an contained angle with the axis body.
Preferably, the variable frequency oscillation assembly is positioned in the liquid containing vessel and is contacted with the fermentation liquid; the variable frequency oscillating assembly comprises at least one variable frequency oscillator; the frequency of the variable frequency oscillation component is 10Hz-90 KHz; the airflow condenser is a condensing fan.
Compared with the prior art, the utility model, its beneficial effect is: the utility model provides a nicotinamide mononucleotide production system has set up frequency conversion oscillation device in nicotinamide mononucleotide's production container, vibrates the subassembly through the frequency conversion, carries out the frequency conversion to the zymotic fluid and vibrates, with zymotic fluid homodisperse. The fermentation liquor after being uniformly dispersed is conveyed to a production container for producing nicotinamide mononucleotide through an airflow condenser at the top of the liquor container, so that the fermentation liquor can be uniformly mixed. Thus, nicotinamide mononucleotide can be ensured to be in a constant production environment during growth for up to one to three years. In addition, the variable frequency oscillation can not only uniformly disperse the fermentation liquor, but also break the cell walls of microorganisms such as bacteria, viruses and spores in the fermentation liquor, lose activity and kill the microorganisms, thereby achieving the bacteriostatic effect, and simultaneously enabling substances in the fermentation liquor to be rapidly dissolved and uniformly distributed in the fermentation liquor.
Drawings
FIG. 1 is a schematic diagram of a nicotinamide mononucleotide production system according to example one;
FIG. 2 is a schematic diagram of a nicotinamide mononucleotide production system of example two;
FIG. 3 is a schematic diagram of a nicotinamide mononucleotide production system of example III;
fig. 4 and 5 are schematic structural diagrams of the stirrer according to the present invention at different viewing angles.
In the figure: 10. nicotinamide mononucleotide; 20. fermentation liquor; 30. uniformly dispersing the fermentation liquor; 50. producing a container; 60. a variable frequency oscillating device; 100. 200, 300, a nicotinamide mononucleotide production system; 110. a liquid containing vessel; 112. a variable frequency oscillating component; 112A, a variable frequency oscillation generator; 114. an airflow condenser; 210. a liquid preparation vessel; 212. an infusion path; 214. a valve; 216. a stirring assembly; 310. an infusion pump; 90. a shaft body; 911. a first frame body; 912. a second frame body; 931. a first stirring plate; 932. a second stirring plate; 921. third stirring plate 922, fourth stirring plate.
Detailed Description
The present invention is further described in detail below with reference to the drawings so that those skilled in the art can implement the invention with reference to the description.
Example one
Referring to FIG. 1, the nicotinamide mononucleotide producing system 100 of this embodiment comprises a production vessel 50 and a variable frequency oscillating device 60 disposed in the production vessel 50.
In production vessel 50, nicotinamide mononucleotide 10 is enzymatically produced. The variable frequency oscillating device 60 comprises a liquid container 110, which is disposed in the production vessel 50 and contains the fermentation liquid 20.
The bottom of the liquid container 110 is provided with a variable frequency oscillating assembly 112, the variable frequency oscillating assembly 112 is immersed in the fermentation liquid 20, and the top of the liquid container 110 is provided with an airflow condenser 114. In a specific application scenario, one or more variable frequency oscillation devices 60 may be disposed in the nicotinamide mononucleotide producing system 100 according to the size of the production container 50. That is, in an application environment, a variable frequency oscillating device 60 may be disposed at the center of the production container 50. In another application, two or more variable frequency oscillating devices 60 may be disposed in the production vessel 50 in a spatially distributed manner. In order to simplify the drawings, only one variable frequency oscillating device 60 is shown in the drawings.
Further, the fermentation liquid 20 can be directly poured into the liquid containing vessel 110 through the gap of the airflow condenser 114; wherein, the fermentation liquid 20 is used for regulating and controlling the production environment of the nicotinamide mononucleotide 10.
Then, the variable frequency oscillation assembly 112 located at the bottom of the liquid container 110 is used for carrying out variable frequency oscillation on the fermentation liquid 20, so that the fermentation liquid 20 is uniformly dispersed.
Specifically, the variable frequency oscillating assembly 112 may include one or more variable frequency oscillation generators 112A, and in the present embodiment, the variable frequency oscillating assembly 112 includes eight variable frequency oscillation generators 112A arranged in an array.
In the production process, the frequency conversion oscillating assembly 112 converts the 50Hz or 60Hz current into mechanical vibration with the same frequency in a manner similar to an ultrasonic generator, and then the mechanical vibration performs frequency conversion oscillation on the fermentation liquid 20 through the frequency conversion oscillating assembly 112.
Further, the variable frequency oscillating assembly 112 includes a variable frequency oscillating transducer (not shown), an amplitude transformer (not shown), a variable frequency oscillating pool (not shown), and a driving power source (not shown). The driving power supply converts current into variable-frequency high-voltage alternating current and transmits the variable-frequency high-voltage alternating current to the variable-frequency oscillation transducer; the frequency conversion oscillation transducer can convert the received electric energy into mechanical energy, namely frequency conversion oscillation; the frequency conversion oscillation transducer can perform back and forth telescopic motion, and the frequency of the telescopic motion is equal to that of the frequency conversion high-voltage alternating current.
In an application environment, the frequency of the frequency conversion oscillation generated by the frequency conversion oscillation component 112 is about less than 90 KHz. When the frequency is less than 50Hz, the effect of uniformly dispersing the fermentation liquid 20 is not good, for example, the average kinetic diameter of the molecules of the uniformly dispersed fermentation liquid 30 is not small enough; when the frequency is greater than about 90KHz, the service life of the entire variable frequency oscillating device 60 is reduced. When the frequency is less than 100Hz, the homogeneously dispersed molecules of the fermentation broth 30 are smaller than in conventional processes and can therefore be retained in the production vessel for a longer time, for example for about 30 minutes. Under an application environment, the average kinetic diameter of the molecules of the uniformly dispersed fermentation liquor 30 is between 1 μm and 20 μm, or between 1 μm and 10 μm, or between 1 μm and 5 μm, and a better wall breaking effect can be achieved by increasing the frequency by 20-90 KHz.
The uniformly dispersed fermentation broth 30 is then transferred to the production vessel 50 through an air flow condenser 114 located at the top of the liquid holding vessel 110, wherein the air flow condenser 114 may be a condensing fan in an application environment. Then, the uniformly dispersed fermentation solution 30 is vaporized by heat exchange with the external air, and rapidly dispersed throughout the production vessel 50.
In the nicotinamide mononucleotide producing system, the variable frequency oscillating device is arranged in a nicotinamide mononucleotide producing container, and the variable frequency oscillating component is used for carrying out variable frequency oscillation on the fermentation liquor to uniformly disperse the fermentation liquor. The fermentation liquor after being uniformly dispersed is conveyed to a production container for producing nicotinamide mononucleotide through an airflow condenser at the top of the liquor container, so that the fermentation liquor can be uniformly mixed. Therefore, this example ensures that nicotinamide mononucleotide can be maintained in a constant production environment during the culturing process.
In addition, the variable frequency oscillation can not only uniformly disperse the fermentation liquor, but also break the cell walls of microorganisms such as bacteria, viruses and spores in the fermentation liquor, lose activity and kill the microorganisms, thereby achieving the bacteriostatic effect, and simultaneously enabling substances in the fermentation liquor to be rapidly dissolved and uniformly distributed in the fermentation liquor.
Example two
Referring to FIG. 2, the nicotinamide mononucleotide producing system 200 of this embodiment comprises a production vessel 50 and a variable frequency oscillator 60 disposed within the production vessel 50.
It should be noted that the same or similar components or devices in fig. 2 are denoted by the same or similar reference numerals, and the materials, configurations and functions thereof are the same or similar to those described above, so that the detailed description thereof will not be repeated.
The difference between the present embodiment and the first embodiment is that the liquid container 110 can be connected to the liquid preparation vessel 210 through the liquid feeding path 212, wherein the liquid feeding path 212 is connected to the upper opening of the sidewall of the liquid container 110, and the liquid feeding path 212 is connected to the lower opening of the sidewall of the liquid preparation vessel 210. Therefore, the fermentation liquid can flow from the dispensing vessel 210 to the inner space of the liquid holding vessel 110 through the liquid feeding path 212.
In an application environment, the level of the lower opening of the sidewall of the dispensing well 210 is higher than the level of the upper opening of the sidewall of the liquid containing well 110. Therefore, the fermentation liquid 20 can flow from the lower opening of the side wall of the liquid distribution vessel 210 to the upper opening of the side wall of the liquid containing vessel 110 through the liquid feeding path 212 without applying any external power. The method of making the lower opening of the sidewall of the dispensing well 210 have a higher level than the upper opening of the sidewall of the liquid container 110 includes disposing a platform (not shown in fig. 2) below the dispensing well 210 to raise the dispensing well 210, but the method is not limited thereto.
Further, a valve 214 may be installed in the liquid feeding path 212 to control the flow of the fermentation liquid 20 from the dispensing vessel 210 to the liquid holding vessel 110.
Wherein, the bottom of the liquid preparation vessel 210 may be provided with a stirring component 216 to stir the fermentation liquid 20, so that the substances in the fermentation liquid 20 can be evenly distributed in the fermentation liquid 20.
In particular, the stirring assembly 216 may be composed of a single stirrer, although in other applications, the stirring assembly 216 may be composed of a plurality of stirrers.
More specifically, the stirrers may be disposed at the bottom of the liquid preparation vessel 210 in a horizontal distribution, and in other application environments, the stirrers may also be disposed in a row along a direction perpendicular to the bottom of the liquid preparation vessel 210 to form an elongated stirring assembly 216.
According to fig. 4 and 5, in order to improve the stirring capacity of the stirring assembly 216, the stirrer comprises a shaft body 90 arranged in the liquid distribution vessel 210 and capable of being rotated by a rotary drive (e.g. a motor); a first frame body 911 and a second frame body 912 which are fixed on the shaft body 90 and basically combined into a rectangular frame; a first agitating plate 931 and a second agitating plate 932 fixed to the shaft 90 and positioned in the rectangular frame; a third agitating plate 921 located inside the rectangular frame and fixed to the first frame 911; and a fourth agitating plate 922 located inside the rectangular frame and fixed to the second frame body 912.
Specifically, the first stirring plate 931, the second stirring plate 932, the third stirring plate 921 and the fourth stirring plate 922 are all defined to be disposed toward the shaft body 90; the first agitating plate 931, the second agitating plate 932, the third agitating plate 921, and the fourth agitating plate 922 are arranged on both sides of the shaft body 90 in a center-symmetric manner.
Further, the first agitating plate 931 and the fourth agitating plate 922 are disposed at an angle between 20 ° and 70 ° with respect to the shaft 90.
In a specific application environment, the number of the first stirring plate 931, the second stirring plate 932, the third stirring plate 921 and the fourth stirring plate 922 is multiple; the first stirring plate 931 and the third stirring plate 921 are located at one side of the shaft 90, and are arranged at intervals; second stirring plate 932 and fourth stirring plate 922 are located at the other side of shaft 90, and both are set up for the looks interval.
This embodiment includes the advantages mentioned above, and at the same time, the nicotinamide mononucleotide producing system 200 is a continuous system, so that the fermentation liquid can be continuously transported from the liquid preparation vessel to the liquid containing vessel via the liquid transporting path, so that the variable frequency oscillating device can continuously generate and transport the uniformly dispersed fermentation liquid to the nicotinamide mononucleotide producing vessel, and the production vessel can be maintained in a constant production environment for a longer period of time.
EXAMPLE III
Referring to FIG. 3, the nicotinamide mononucleotide producing system 200 of this embodiment comprises a production vessel 50 and a variable frequency oscillator 60 disposed within the production vessel 50.
It should be noted that the same or similar components or devices in fig. 3 are denoted by the same or similar reference numerals, and the materials, configuration and functions thereof are the same or similar to those described above, so that the detailed description thereof will not be repeated.
The difference between the present embodiment and the second embodiment is that the level of the lower opening of the sidewall of the liquid distribution vessel 210 is lower than the level of the upper opening of the sidewall of the liquid containing vessel 110. Therefore, the fermentation liquid 20 needs to flow from the lower opening of the side wall of the preparation vessel 210 to the upper opening of the side wall of the liquid holding vessel 110 by an external power. Specifically, the infusion pump 310 may be provided in the infusion path 212 to transfer the fermentation liquid 20 from the dispensing dish 210 to the liquid containing dish 110.
In addition to the advantages described above, since the nicotinamide mononucleotide producing system 300 does not need to use additional equipment to raise the dispensing vessel 210, the nicotinamide mononucleotide producing system 300 occupies a smaller volume in the production vessel 50, and the space for growing nicotinamide mononucleotide 10 in the production vessel 50 becomes larger.
To sum up, the utility model discloses set up frequency conversion oscillation device in nicotinamide mononucleotide's production container, vibrate the subassembly through the frequency conversion to basically for being less than 100 Hz's frequency, carry out the frequency conversion to the zymotic fluid and vibrate, with zymotic fluid homodisperse. The fermentation liquor after being uniformly dispersed is conveyed to a production container for producing nicotinamide mononucleotide through an airflow condenser at the top of the liquor container, so that the fermentation liquor can be uniformly mixed.
In addition, the variable frequency oscillation can not only uniformly disperse the fermentation liquor, but also break the cell walls of microorganisms such as bacteria, viruses, spores and the like in the fermentation liquor, lose activity and kill the microorganisms, thereby achieving the bacteriostatic effect, and simultaneously enabling substances in the fermentation liquor to be rapidly dissolved and uniformly distributed in the fermentation liquor. Because this application can become continuous from batch type, the appearance liquid ware of frequency conversion oscillation device passes through the infusion route and joins in marriage the liquid ware and be connected, makes the fermentation liquor can be via the infusion route, and the liquid ware is carried to appearance liquid ware from joining in marriage continuously, makes frequency conversion oscillation device constantly produce the fermentation liquor of homodisperse to conveying to nicotinamide mononucleotide production container, making the production container can maintain under the production environment of invariant for a long time.
While the embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields of application suitable for this invention, and further modifications may be readily made by those skilled in the art, and the invention is therefore not limited to the specific details and illustrations shown and described herein, without departing from the general concept defined by the claims and their equivalents.
Claims (10)
1. A nicotinamide mononucleotide production system, comprising:
a production vessel capable of enzymatically producing nicotinamide mononucleotide inside thereof;
a liquid container arranged in the production container for containing fermentation liquid for producing the nicotinamide mononucleotide;
the variable frequency oscillation assembly is arranged at the bottom of the liquid containing vessel and generates variable frequency oscillation to uniformly disperse the fermentation liquid; and
and the airflow condenser is arranged at the top of the liquid containing vessel, and is used for discharging the uniformly dispersed fermentation liquid out of the liquid containing vessel and conveying the fermentation liquid into the production container.
2. The nicotinamide mononucleotide production system of claim 1, further comprising a dispensing vessel for preparing said fermentation broth, wherein said dispensing vessel is connected to said holding vessel by an infusion line.
3. The nicotinamide mononucleotide production system of claim 2, wherein said infusion pathway is provided with a valve to open or close said infusion pathway to control the flow of said fermentation broth between said dispensing dish and said holding dish.
4. The nicotinamide mononucleotide production system of claim 3, wherein said infusion path is connected to an upper opening of the side wall of said liquid containing dish and to a lower opening of the side wall of said liquid dispensing dish.
5. The nicotinamide mononucleotide production system of claim 4, wherein a level of said lower opening of said side wall of said dispensing dish is higher than a level of said upper opening of said side wall of said liquid holding dish.
6. The nicotinamide mononucleotide production system of claim 4, wherein the level of said lower opening of the side wall of said dispensing dish is lower than the level of said upper opening of the side wall of said holding dish;
wherein, an infusion pump is arranged on the infusion passage to convey the fermentation liquid from the bottom of the liquid preparation vessel to the liquid containing vessel through the infusion passage.
7. The nicotinamide mononucleotide production system of claim 2, wherein a stirring assembly is disposed at the bottom of the dispensing dish;
wherein the stirring assembly consists of at least one stirrer.
8. The nicotinamide mononucleotide production system of claim 7, wherein said agitator comprises
A rotating shaft body;
the first frame body and the second frame body are fixed on the shaft body and basically combined into a rectangular frame;
the first stirring plate and the second stirring plate are fixed on the shaft body and are positioned in the rectangular frame;
the third stirring plate is positioned in the rectangular frame and fixed on the first frame body; and
and the fourth stirring plate is positioned in the rectangular frame and is fixed on the second frame body.
9. The nicotinamide mononucleotide production system of claim 8, wherein said first agitation plate, said second agitation plate, said third agitation plate, and said fourth agitation plate are all defined to be disposed toward said shaft;
the first stirring plate, the second stirring plate, the third stirring plate and the fourth stirring plate are arranged on two sides of the shaft body in a centrosymmetric manner;
the first stirring plate and the fourth stirring plate are both configured to form an included angle with the shaft body.
10. The nicotinamide mononucleotide production system of claim 1, wherein said variable frequency oscillating assembly is located in said liquid holding vessel and in contact with said fermentation broth; the variable frequency oscillating assembly comprises at least one variable frequency oscillator; the power of the variable frequency oscillation component is less than 90 KHz; the airflow condenser is a condensing fan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020444555.6U CN212128187U (en) | 2020-03-31 | 2020-03-31 | Nicotinamide mononucleotide production system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020444555.6U CN212128187U (en) | 2020-03-31 | 2020-03-31 | Nicotinamide mononucleotide production system |
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| CN212128187U true CN212128187U (en) | 2020-12-11 |
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| CN202020444555.6U Active CN212128187U (en) | 2020-03-31 | 2020-03-31 | Nicotinamide mononucleotide production system |
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