CN207958349U - Bioreactor and its system - Google Patents

Abstract

The utility model is related to biological equipment technology field more particularly to a kind of bioreactor and its systems.The bioreactor includes retort, the agitating device being arranged in the retort and the inlet duct being arranged outside the retort；The agitating device is connect with the tank bottom of the retort, and the agitating shaft of the agitating device is located on the axis of the retort；At least one surface layer air admission hole is provided on the side wall of the retort；It is provided with gas vent on the head cover of the retort；The output end of the inlet duct is connected to the surface layer air admission hole；Height of the surface layer air admission hole apart from the tank bottom presets the depth of culture solution not less than the retort.The bio-reaction system includes air supply system and bioreactor.The purpose of this utility model is to provide bioreactor and its systems to shorten residence time of the gas generated when culture products in bioreactor so that the gas generated when culture products in bioreactor can quickly be discharged.

Description

Bioreactor and its system

Technical field

The utility model is related to biological equipment technology field more particularly to a kind of bioreactor and its systems.

Background technology

Bioreactor (English：Bioreactor) refer to any manufacture that bioactivity environment is provided or engineering equipment, It is to carry out the apparatus system of biochemical reaction in vitro using biological function possessed by enzyme or organism (such as microorganism).It is A kind of biological function analog machine.Have in terms of drinks, medicine production, concentration jam, fruit juice fermentation, organic pollutant degradation important Using.In one case, bioreactor is one and be related to biology or biochemical activity substance by specifically giving birth to The container for the chemical process that object is produced.This process can aerobic progress or anaerobic progress.These bioreactors Usually cylindrical, volume is raised to several kilolitres etc. from several, is often made of 316L stainless steels.

Bioreactor has various forms, so that bioreactor is run well, it is necessary to first to biological respinse Device and response feature have deep understanding, and here it is the concepts of bioreactor engineering.Bioreactor engineering focuses on postgraduate The characteristic of object reactor itself, such as its relationship that structurally and operationally mode, operating condition form cellular morphology, growth, product. It is combined with biological respinse engineering, solves the selection of the best bioreactor, optimum operation condition of various biological respinses jointly Problem.

It carries out often generating carbon dioxide gas in cell cultivation process in bioreactor；The carbon dioxide gas needs It to be rapidly discharged bioreactor, the carbon dioxide gas cognition otherwise accumulated in bioreactor is dissolved in culture simultaneously The pH value of culture is set to accelerate to decline；There is this situation, needs to be added alkaline matter in culture solution (for example, sodium bicarbonate Or sodium hydroxide) maintain the pH value of cell normal growth；But alkaline matter is added moment and can cause to damage to cell, Increasing for culture solution osmotic pressure is will also result in, the stabilization of cell culture environment is destroyed, is unfavorable for cell growth.

Therefore, the application provides a kind of new bioreactor and its system regarding to the issue above, can quickly be discharged Carbon dioxide gas in bioreactor shortens residence time of the carbon dioxide in bioreactor.

Utility model content

The purpose of this utility model is to provide bioreactors, culture products in bioreactor can quickly be discharged When the gas that generates, shorten residence time of the gas generated when culture products in bioreactor.

The purpose of this utility model also resides in offer bio-reaction system, is cultivated with can quickly be discharged in bioreactor The gas generated when product shortens residence time of the gas generated when culture products in bioreactor.

Based on above-mentioned first purpose, bioreactor provided by the utility model, including retort, setting are in the reaction Agitating device in tank and the inlet duct being arranged outside the retort；

The agitating device is connect with the tank bottom of the retort, and the agitating shaft of the agitating device is located at the reaction On the axis of tank；

At least one surface layer air admission hole is provided on the side wall of the retort；The row of being provided on the head cover of the retort Stomata；

The output end of the inlet duct is connected to the surface layer air admission hole；

Height of the surface layer air admission hole apart from the tank bottom presets the depth of culture solution not less than the retort.

The optional technical solution of the utility model is height of the surface layer air admission hole apart from the tank bottom, at least above The retort presets the upper liquid level 30cm-70cm of nutrient solution volume.

The optional technical solution of the utility model is height of the surface layer air admission hole apart from the tank bottom, at least above The retort presets the upper liquid level 50cm of nutrient solution volume.

The optional technical solution of the utility model is that the quantity of the surface layer air admission hole is multiple；Multiple surface layers into Stomata is spaced setting successively along the axial direction of the retort.

The optional technical solution of the utility model is, the agitating device include agitating shaft and with the stirring axis connection Stirring blade；

The bottom of the retort is fixedly connected with driving motor；The driving motor is drivingly connected the agitating shaft, with The stirring blade is set to be rotated with the agitating shaft.

The optional technical solution of the utility model is that the quantity of the stirring blade is three groups；Stirring blade described in three groups Axial direction along the agitating shaft is spaced setting successively；

Along the axial direction of the agitating shaft, the stirring blade described in one group of centre, which is arranged, can make the culture solution along first party To flowing, stirring blade can be such that the culture solution flows in a second direction described in other two groups, the first direction and described the Two directions are opposite.

The optional technical solution of the utility model be provided on the inner wall of the retort it is corresponding to the stirring blade Baffle；

The baffle is arranged with the stirring blade interval；

The quantity of the baffle is multiple, and multiple baffles are uniformly arranged along the inner wall of the retort；

The plate face of the baffle and the radial direction of the retort are arranged in the angle of 20 degree of -50 degree.

The optional technical solution of the utility model is that the tank bottom of the retort is provided with leakage fluid dram.

The optional technical solution of the utility model is that the side wall of the retort has interlayer；It is provided in the interlayer Heat medium；

The side wall of the retort is provided with the loop head and loop exit for being connected to the interlayer.

Based on above-mentioned second purpose, bio-reaction system provided by the utility model, including air supply system and the life Object reactor；

The air supply system is connected to the inlet duct of the bioreactor.

The beneficial effects of the utility model：

Bioreactor provided by the utility model, including retort, agitating device and inlet duct；Pass through agitating device Stir culture liquid so that the gas in culture solution to be discharged out of culture solution, such as will carry out cell cultivation process in retort Middle generation carbon dioxide gas is discharged from culture solution；It is connected to surface layer air admission hole by inlet duct, to be blown into retort The gases such as filtrated air reduce the gas concentration that culture solution generates, and with gases such as filtrated airs from the head cover of retort Gas vent is discharged, such as carries out generating carbon dioxide gas in cell cultivation process with gases such as filtrated airs in retort from row Retort is discharged in stomata；The depth of culture solution is preset not less than retort by height of the surface layer air admission hole apart from tank bottom, so that The gas that the gases such as the filtrated air being blown into retort are preferably generated with culture solution contacts, consequently facilitating reducing culture solution The concentration of the gas of generation reduces residence time of the gas of incubation generation in retort；Such as it can quickly be discharged Carbon dioxide gas in retort reduces residence time of the carbon dioxide in retort.

Bio-reaction system provided by the utility model, including air supply system and bioreactor, having quickly to arrange Go out the gas that culture products generate in bioreactor, when reducing stop of the gas of incubation generation in bioreactor Between the advantages that.

Description of the drawings

It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art Specific implementation mode or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art Under the premise of labour, other drawings may also be obtained based on these drawings.

Fig. 1 is the structural schematic diagram for the bioreactor that the utility model embodiment one provides；

Fig. 2 is another structural schematic diagram for the bioreactor that the utility model embodiment one provides；

Fig. 3 is another structural schematic diagram for the bioreactor that the utility model embodiment one provides；

Fig. 4 is the vertical view of the retort for the bioreactor that the utility model embodiment one provides.

Icon：100- retort；110- tank bottoms；120- head covers；The surface layers 130- air admission hole；140- gas vents；150- baffles； 160- leakage fluid drams；200- agitating devices；210- agitating shafts；220- stirring blades；300- inlet ducts；400- driving motors.

Specific implementation mode

The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally The every other embodiment that field those of ordinary skill is obtained without making creative work, belongs to this practicality Novel protected range.

It is in the description of the present invention, it should be noted that term "center", "upper", "lower", "left", "right", " perpendicular Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only The utility model and simplifying describes for ease of description, do not indicate or imply the indicated device or element must have it is specific Orientation, with specific azimuth configuration and operation, therefore should not be understood as limiting the present invention.In addition, term " the One ", " second ", " third " are used for description purposes only, and are not understood to indicate or imply relative importance.

In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally Connection；It can be mechanical connection, can also be electrical connection；Can be directly connected, can also indirectly connected through an intermediary, It can be the connection inside two elements.For the ordinary skill in the art, it can understand above-mentioned art with concrete condition The concrete meaning of language in the present invention.

Embodiment one

Shown in Fig. 1-Fig. 4, a kind of bioreactor is present embodiments provided；Fig. 1-Fig. 3 is implemented for the utility model Three kinds of structural schematic diagrams of the bioreactor that example provides；Fig. 4 is the anti-of the bioreactor that the utility model embodiment provides Answer the vertical view of tank.Wherein, Fig. 2 shows culture solution.

Shown in Fig. 1-Fig. 4, bioreactor provided in this embodiment, for cultivating cell；The bioreactor packet Include retort 100, the agitating device being arranged in retort 100 200 and the inlet duct 300 being arranged outside retort 100.

Agitating device 200 is connect with the tank bottom 110 of retort 100, and the agitating shaft 210 of agitating device 200 is located at reaction On the axis of tank 100.

At least one surface layer air admission hole 130 is provided on the side wall of retort 100；It is arranged on the head cover 120 of retort 100 There is gas vent 140.The output end of inlet duct 300 is connected to surface layer air admission hole 130；Optionally, the output end of inlet duct 300 is defeated Go out to compress filtrated air to surface layer air admission hole 130.The gases such as compression filtrated air to lengthen the output of inlet duct 300 are being cultivated The distance that the ullage of liquid is walked, it is easy to carry go out the gas that generates of the culture solutions such as more carbon dioxide；

Height of the surface layer air admission hole 130 apart from tank bottom 110 presets the depth of culture solution not less than retort 100.Namely Say that surface layer air admission hole 130 is located at the top that retort 100 presets culture solution.

Bioreactor described in the present embodiment, including retort 100, agitating device 200 and inlet duct 300；Pass through 200 stir culture liquid of agitating device, so that the gas in culture solution is discharged out of culture solution, such as by retort 100 into Carbon dioxide gas is generated in row cell cultivation process to be discharged from culture solution；It is connected to surface layer air admission hole by inlet duct 300 130, to be blown into the gases such as filtrated air into retort 100, the gas concentration that culture solution generates is reduced, and with filtrated air etc. Gas is discharged from the gas vent 140 on the head cover 120 of retort 100, such as carries out producing in cell cultivation process in retort 100 Retort 100 is discharged from gas vent 140 with gases such as filtrated airs in raw carbon dioxide gas；Pass through 130 distance of surface layer air admission hole The height of tank bottom 110 presets the depth of culture solution not less than retort 100, so that the filtrated air being blown into retort 100 The gas that equal gases are preferably generated with culture solution contacts, consequently facilitating reducing the concentration for the gas that culture solution generates, reduces training Residence time of the gas that the process of supporting generates in retort 100；Such as the carbon dioxide in retort 100 can quickly be discharged Gas reduces residence time of the carbon dioxide in retort 100.

Bioreactor described in the present embodiment, reduces residence time of the carbon dioxide in retort 100, reduce or Person avoids carbon dioxide gas and is dissolved in culture solution and the pH value of culture solution is made to accelerate the situation declined, also reduces or keeps away Exempt from alkaline matter is added in culture solution and maintained the pH value of cell normal growth, cell culture environment is made more to stablize, from And promote cell quality.

In the alternative of the present embodiment, height of the surface layer air admission hole 130 apart from tank bottom 110, at least above retort 100 The upper liquid level 30cm-70cm of default nutrient solution volume.It is at least above retort 100 by surface layer air admission hole 130 and presets culture solution 30cm-70cm, to ensure that the gas that surface layer air admission hole 130 exports is not blown and beaten directly to culture solution, to reduce or avoid thin It will produce more foam in born of the same parents' incubation.

In the alternative of the present embodiment, height of the surface layer air admission hole 130 apart from tank bottom 110, at least above retort 100 The upper liquid level 50cm of default nutrient solution volume；Further ensure that the gas that surface layer air admission hole 130 exports is not blown and beaten directly to training Nutrient solution, to reduce or avoid to will produce more foam in cell cultivation process.

Contrast table

As can be seen from the above table, the alkaline matter that the bioreactor of the prior art fills into is more, and culture osmotic pressure increases, A small amount of cell will appear loose situation.The bioreactor of the present embodiment fills into the opposite reduction of alkaline matter, can also reduce Cost.

In the alternative of the present embodiment, the quantity of surface layer air admission hole 130 is multiple；Multiple surface layer air admission holes 130 are along anti- The axial direction of tank 100 is answered to be spaced setting successively.By the way that multiple surface layer air admission holes 130 are arranged, to adapt to the not co-content of retort 100 Culture solution culture cell.

The quantity of surface layer air admission hole 130 for example can be 2,3,5,6 or 7 etc..Optionally, referring to Fig. 1 Shown, the quantity of surface layer air admission hole 130 is 2.

In the alternative of the present embodiment, agitating device 200 includes agitating shaft 210 and the stirring being connect with agitating shaft 210 Blade 220.

Optionally, agitating device 200 uses magnetic agitation.

The tank bottom of retort 100 is fixedly connected with driving motor 400；Driving motor 400 is drivingly connected agitating shaft 210, with Stirring blade 220 is set to be rotated with agitating shaft 210, to pass through 220 stir culture liquid of stirring blade.

In the alternative of the present embodiment, the quantity of stirring blade 220 is three groups；Three groups of stirring blades 220 are along agitating shaft 210 axial direction is spaced setting successively.

Along the axial direction of agitating shaft 210, the stirring blade 220 described in one group of centre, which is arranged, can make the culture solution along first Direction is flowed, and stirring blade 220 can be such that the culture solution flows in a second direction described in other two groups, the first direction and institute It is opposite to state second direction.That is, be arranged one group of intermediate stirring blade 220 make the flow direction of the culture solution with other two Group stirring blade 220 makes the flow direction of the culture solution on the contrary, so that culture solution stirring is more uniform.For example, being arranged in Between one group of stirring blade 220 it is opposite with the setting angle direction of other two groups of stirring blades 220；For example, being arranged in centre One group of stirring blade 220 makes the culture solution towards the flowing of direction obliquely downward (being in pressed status), other two groups of stirring blades 220 make institute State culture solution obliquely to flowing (in the state of raising up)；Vice versa.

Optionally, driving motor 400 includes first motor and the second motor；First motor is drivingly connected intermediate one group and stirs Blade 220 is mixed, the second motor is drivingly connected other two groups of stirring blades 220.Optionally, the quantity of driving motor 400 is one, One driving motor 400 connects three groups of stirring blades 220 by actuator drives.

Optionally, three groups of stirring blades 220 are respectively the first paddle, the second paddle and third paddle；First stirs The axial direction that leaf, the second paddle and third paddle are mixed along agitating shaft 210 is spaced setting successively, wherein the first paddle is close The head cover 120 of retort 100, tank bottom 110 of the third paddle close to retort 100；First paddle, the second paddle and The direction of rotation of three paddles may be the same or different.Preferably, the first paddle, the second paddle and third stirring The direction of rotation of leaf is identical, and the second paddle is opposite with the first paddle, the setting angle direction of third paddle；So that second Paddle stirring after in raise up either the first paddle of pressed status and third paddle stirring after in push or the shape that raises up State, so that culture solution stirring is more uniform.

Shown in Fig. 1-Fig. 3, in the alternative of the present embodiment, it is provided on the inner wall of retort 100 and paddle 220 corresponding baffle 150 of piece；By baffle 150 to weaken the flow velocity of the culture solution of 100 inner wall of retort, so that culture solution Environment is more stablized, and gas-liquid mixed is more uniform.

Baffle 150 is arranged with the interval of stirring blade 220；Baffle 150 is touched in rotation to avoid stirring blade 220, To improve the security performance of bioreactor.

Optionally, the quantity of baffle 150 is multiple, and multiple baffles 150 are uniformly arranged along the inner wall of retort 100；Baffle 150 quantity for example can be 2,3,5,6 or 7 etc..

The plate face of baffle 150 is arranged with the radial direction of retort 100 in the angle of 20 degree of -50 degree, is reacted with further decrease The flow velocity of the culture solution of 100 inner wall of tank, so that the environment of culture solution is more stablized.The diameter of the plate face and retort 100 of baffle 150 Angle between for example can be 20 degree, 25 degree, 35 degree, 40 degree or 50 degree etc..

In the alternative of the present embodiment, the tank bottom 110 of retort 100 is provided with leakage fluid dram 160.By leakage fluid dram 160, For discharging and/or blowdown.

In the alternative of the present embodiment, the side wall of retort 100 has interlayer.

Optionally, it is provided with heat medium in the interlayer of the side wall of retort 100；Heat medium for example can be water or Conduction oil.The side wall of retort 100 is provided with the loop head and loop exit for being connected to the interlayer；Heat medium is through being recycled into Mouth flows into interlayer, flows out interlayer through loop exit.For example, flowing into interlayer from loop head by the water of heating or cooling It is interior, interlayer is flowed out from loop exit, to realize the heating, heat preservation, cooling of retort 100.

Optionally, the heat medium in interlayer is completed by heating device.By heat medium, to heat retort 100, so that cell is in a suitable temperature and is cultivated.

Optionally, heating device is electric heating wire or steam-plate type heat exchanger.

Embodiment two

Embodiment two provides a kind of bio-reaction system, which includes the bioreactor described in embodiment one, The technical characteristic of bioreactor disclosed in embodiment one is also applied for the embodiment, one published biological respinse of embodiment The technical characteristic of device is not repeated to describe.

Bio-reaction system provided in this embodiment, including air supply system and bioreactor.Air supply system may include Air feed pump.

Air supply system is connected to the inlet duct of bioreactor.By air supply system with to the retort of bioreactor The gases such as filtrated air are inside blown into, to reduce the density of carbon dioxide gas generated during culture products, so that incubation The carbon dioxide gas of middle generation is discharged with air etc. from the gas vent on the head cover of retort.

Bio-reaction system described in the present embodiment, by agitating device stir culture liquid, so as to by the gas in culture solution It is discharged out of culture solution, such as generation carbon dioxide gas in cell cultivation process will be carried out in retort and arranged from culture solution Go out；It is connected to surface layer air admission hole by inlet duct, to be blown into the gases such as filtrated air into retort, reduces culture Finished product processThe The gas concentration of middle generation, and be discharged from the gas vent on the head cover of retort with gases such as filtrated airs, such as in retort It carries out generation carbon dioxide gas in cell cultivation process and retort is discharged from gas vent with gases such as filtrated airs；Pass through surface layer Height of the air admission hole apart from tank bottom presets the depth of culture solution not less than retort, so that the filtrated air being blown into retort The gas that equal gases are preferably generated with culture solution contacts, consequently facilitating reducing the concentration for the gas that culture solution generates, reduces training Residence time of the gas that nutrient solution generates in retort；Such as the carbon dioxide gas in retort can quickly be discharged, subtract Few residence time of the carbon dioxide in retort.

Bio-reaction system described in the present embodiment has the advantages that bioreactor described in embodiment one, one institute of embodiment The advantages of disclosed bioreactor, is not repeated to describe herein.

Finally it should be noted that：The above various embodiments is only to illustrate the technical solution of the utility model, rather than limits it System；Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should Understand：It still can be with technical scheme described in the above embodiments is modified, either to which part or whole Technical characteristic carries out equivalent replacement；And these modifications or replacements, this practicality that it does not separate the essence of the corresponding technical solution are new The range of each embodiment technical solution of type.

Claims (10)

1. a kind of bioreactor, which is characterized in that including retort, the agitating device being arranged in the retort and setting Inlet duct outside the retort；

The agitating device is connect with the tank bottom of the retort, and the agitating shaft of the agitating device is located at the retort On axis；

At least one surface layer air admission hole is provided on the side wall of the retort；Exhaust is provided on the head cover of the retort Hole；

The output end of the inlet duct is connected to the surface layer air admission hole；

Height of the surface layer air admission hole apart from the tank bottom presets the depth of culture solution not less than the retort.

2. bioreactor according to claim 1, which is characterized in that height of the surface layer air admission hole apart from the tank bottom Degree, at least above described retort preset the upper liquid level 30cm-70cm of nutrient solution volume.

3. bioreactor according to claim 2, which is characterized in that height of the surface layer air admission hole apart from the tank bottom Degree, at least above described retort preset the upper liquid level 50cm of nutrient solution volume.

4. bioreactor according to claim 1, which is characterized in that the quantity of the surface layer air admission hole is multiple；It is more A surface layer air admission hole is spaced setting successively along the axial direction of the retort.

5. bioreactor according to claim 1, which is characterized in that the agitating device include agitating shaft and with it is described Stir the stirring blade of axis connection；

The bottom of the retort is fixedly connected with driving motor；The driving motor is drivingly connected the agitating shaft, so that institute Stirring blade is stated to rotate with the agitating shaft.

6. bioreactor according to claim 5, which is characterized in that the quantity of the stirring blade is three groups；Three groups The stirring blade is spaced setting successively along the axial direction of the agitating shaft；

Along the axial direction of the agitating shaft, the stirring blade described in one group of centre, which is arranged, can make the culture solution along first direction stream Dynamic, stirring blade can be such that the culture solution flows in a second direction described in other two groups, the first direction and the second party To opposite.

7. bioreactor according to claim 6, which is characterized in that be provided on the inner wall of the retort with it is described The corresponding baffle of stirring blade；

The baffle is arranged with the stirring blade interval；

The quantity of the baffle is multiple, and multiple baffles are uniformly arranged along the inner wall of the retort；

The plate face of the baffle and the radial direction of the retort are arranged in the angle of 20 degree of -50 degree.

8. bioreactor according to claim 1, which is characterized in that the tank bottom of the retort is provided with leakage fluid dram.

9. bioreactor according to claim 1, which is characterized in that the side wall of the retort has interlayer；It is described Heat medium is provided in interlayer；

The side wall of the retort is provided with the loop head and loop exit for being connected to the interlayer.

10. a kind of bio-reaction system, which is characterized in that including air supply system and claim 1-9 any one of them biologies Reactor；

The air supply system is connected to the inlet duct of the bioreactor.