CN114806810A - Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor and application thereof - Google Patents
Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor and application thereof Download PDFInfo
- Publication number
- CN114806810A CN114806810A CN202210670062.8A CN202210670062A CN114806810A CN 114806810 A CN114806810 A CN 114806810A CN 202210670062 A CN202210670062 A CN 202210670062A CN 114806810 A CN114806810 A CN 114806810A
- Authority
- CN
- China
- Prior art keywords
- tank
- fermentation
- gas
- oxygen
- micro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002101 nanobubble Substances 0.000 title claims abstract description 97
- 239000001301 oxygen Substances 0.000 title claims abstract description 81
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 81
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 80
- 238000010564 aerobic fermentation Methods 0.000 title claims abstract description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 174
- 230000004151 fermentation Effects 0.000 claims abstract description 174
- 239000007788 liquid Substances 0.000 claims abstract description 88
- 238000003756 stirring Methods 0.000 claims abstract description 27
- 210000001822 immobilized cell Anatomy 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 62
- 239000007789 gas Substances 0.000 claims description 59
- 238000000926 separation method Methods 0.000 claims description 33
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 32
- 238000002156 mixing Methods 0.000 claims description 28
- 235000019766 L-Lysine Nutrition 0.000 claims description 16
- 239000004472 Lysine Substances 0.000 claims description 16
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 241000186226 Corynebacterium glutamicum Species 0.000 claims description 11
- 210000004027 cell Anatomy 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 9
- 238000010907 mechanical stirring Methods 0.000 claims description 8
- 229920000742 Cotton Polymers 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000010899 nucleation Methods 0.000 claims description 5
- 238000010008 shearing Methods 0.000 claims description 5
- 239000008223 sterile water Substances 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000012262 fermentative production Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 6
- 230000035755 proliferation Effects 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 7
- 239000012071 phase Substances 0.000 description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011218 seed culture Methods 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 229910001882 dioxygen Inorganic materials 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229960003966 nicotinamide Drugs 0.000 description 2
- 235000005152 nicotinamide Nutrition 0.000 description 2
- 239000011570 nicotinamide Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 2
- 239000012808 vapor phase Substances 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002926 oxygen Chemical class 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000006385 ozonation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000005501 phase interface Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Landscapes
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an oxygen micro-nano bubble enhanced aerobic fermentation bioreactor and application thereof. Oxygen and seed liquid form oxygen micro-nano bubbles through the micro-nano bubble generator, the oxygen micro-nano bubbles enter the fermentation tank, and oxygen is increased through the cooperation of the stirring paddle and the oxygen micro-nano bubbles, so that the oxygen mass transfer efficiency of a fermentation system is improved. In addition, the circulation of the oxygen micro-nano bubbles can reduce the size of the bubbles, enhance the stability of the bubbles in the solution and prolong the existence time while keeping the dissolved oxygen content of the fermentation liquor, thereby being beneficial to the growth and proliferation of immobilized cells, improving the self-renewal capacity of microorganisms in a fermentation tank and further playing the role of enhancing the continuous fermentation degree of the immobilized cells.
Description
Technical Field
The invention belongs to the field of microbial fermentation, and particularly relates to an oxygen micro-nano bubble enhanced aerobic fermentation bioreactor.
Background
In aerobic submerged fermentation, the supply of oxygen is often one of the important limiting factors for the success of the fermentation. For aerobic microorganisms, molecular oxygen must be present for growth, but because oxygen has a low solubility in water, and thus in the fermentation broth, continuous aeration and agitation are required to meet the oxygen demand of the aerobic microorganisms.
The micro-nano bubble is a micro-nano bubble, and has small particle size compared with the common bubble, so the micro-nano bubble has some special advantages, such as long existence time, high mass transfer efficiency, slow rising speed, high Zeta potential formed by surface charge and the like. The characteristics enable micro-nano bubbles to be widely applied to the fields of degradation of pollutants in water, aquaculture, agricultural soil improvement and the like in recent years, and Luliang et al (Shanghai agricultural science and technology, 2017, 06: 52+73) irrigate rice by using bubble water generated by a nano-micro gas-liquid dispersion phase interface mixing generator, so that the activity of the root system of the rice can be improved, the effective spike number, the spike number and the thousand kernel weight are increased, the yield is improved, the fertilizer consumption can be reduced, the agricultural non-point source pollution is favorably controlled, and the ecological environment is protected. Yuanling et al (Industrial Water treatment, 2020, 40 (07): 47-50) utilize micro-nano bubbles in combination with an ozonization technology to treat sludge, and compared with common ozone bubbles, the micro-nano bubbles of ozone have a higher sludge reduction effect, and the sludge reduction rate can reach about 15%. CN 114180670A provides an ozone micro-nano bubble wastewater treatment system and a wastewater treatment method, the size of micro-nano bubbles is reduced through primary gas dissolving and secondary gas dissolving processes, and the stability of the micro-nano bubbles in water is enhanced, so that the wastewater treatment effect is improved.
Disclosure of Invention
The purpose of the invention is as follows: the potential application value of the micro-nano bubbles is proved by existing literature reports, but the micro-nano bubbles are not applied to a fermentation system of aerobic microorganisms at present, so that the problems existing in the process of immobilized fermentation of the aerobic microorganisms are solved, for example: the invention provides an oxygen micro-nano bubble enhanced aerobic fermentation bioreactor which is used for improving the continuous fermentation performance of immobilized cells of aerobic microorganisms.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
an oxygen micro-nano bubble enhanced aerobic fermentation bioreactor comprises an oxygen generator, a seed tank, a gas-liquid mixing pump, a micro-nano bubble generator, a fermentation tank, a batching tank, a gas-liquid separation tank and a fermentation product collecting tank;
the gas-liquid mixing pump, the micro-nano bubble generator, the fermentation tank, the gas-liquid separation tank and the fermentation product collecting tank are sequentially connected through pipelines; the batching tank is connected with the fermentation tank;
the oxygen generator and the seed tank are respectively connected to a first inlet and a second inlet of the gas-liquid mixing pump, oxygen and seed liquid are fed into the gas-liquid mixing pump to be mixed, then are fed into the micro-nano bubble generator to form oxygen micro-nano bubbles through shearing, and then are fed into the fermentation tank to be fermented together with the raw material fed by the batching tank, and fermentation products are separated by the gas-liquid separating tank and then are fed into the fermentation product collecting tank.
Specifically, the micro-nano bubble generator comprises a nano bubble pump, a safety valve, a pressure tank and a bubble atomizer; the feed inlet of the nano bubble pump is connected with the discharge outlet of the gas-liquid mixing pump; the nano bubble pump, the pressure tank and the bubble atomizer are sequentially connected; the discharge hole of the bubble atomizer is connected with the feed inlet of the fermentation tank; the nano bubble pump is connected with the pressure tank through a gas circulating pipe, the excessive oxygen in the pressure tank is returned to the nano bubble pump, and the safety valve is arranged on the gas circulating pipe.
Furthermore, a discharge hole of the bubble atomizer is connected with a feed inlet below the side surface of the fermentation tank, and a water lifting pump is arranged on the connecting pipeline; the gas outlet at the top of the fermentation tank is connected to the pressure tank through a gas circulating pipe, the redundant oxygen in the fermentation tank is returned to the pressure tank, and the gas circulating pipe is provided with a suction pump.
Specifically, the fermentation tank is a mechanical stirring type fermentation tank, a feed inlet below the side surface of the fermentation tank is connected with the micro-nano bubble generator, a feed inlet at the top is connected with the batching tank, and a discharge outlet at the bottom is connected with the gas-liquid separation tank;
a stirring paddle is arranged in the fermentation tank and is connected with a motor positioned above the top of the fermentation tank, and the motor drives the stirring paddle to stir the materials in the fermentation tank;
a cylindrical and hollow silk screen fixer is arranged in the center of the reaction cavity in the fermentation tank and used for fixing cotton fibers; the stirring paddle penetrates through the hollow part in the middle of the screen fixer;
the fermentation tank is also provided with a heat exchanger, and the temperature of the fermentation liquid in the fermentation tank is adjusted through the heat exchanger.
Furthermore, the heat exchanger is fixed on the inner wall of the reaction cavity of the mechanical stirring fermentation tank by selecting a plurality of built-in coil pipe heat exchangers, the heat exchanger is connected with a water tank outside the fermentation tank through a water pipe, a water pump is arranged on the water pipe, and the pre-cooled sterile water in the water tank is sent into the coil pipe in the fermentation tank through the water pump to exchange heat with fermentation liquor in the tank.
Furthermore, a first gas phase balance pipe is arranged on a pipeline connecting a feed inlet at the top of the fermentation tank and the batching tank; and a second gas phase balance pipe is arranged on a pipeline connecting the bottom discharge port of the fermentation tank and the gas-liquid separation tank.
Furthermore, a gas filter is arranged on a pipeline connecting the oxygen generator and the gas-liquid mixing pump, so that the seed liquid is prevented from being polluted by the mixed bacteria in the oxygen.
Specifically, a feed inlet at the bottom of the gas-liquid separation tank is connected with a discharge outlet of the fermentation tank, a middle separation feed inlet is connected with a fermentation product collecting tank, and a gas outlet at the top of the gas-liquid separation tank is connected with a water separator.
The water separator is provided with a water outlet, a gas inlet, a gas outlet, a circulating gas outlet and a circulating gas inlet, wherein the circulating gas inlet is communicated with the gas outlet at the top of the gas-liquid separation tank, the circulating gas outlet is communicated with the gas inlet in the water separator body, the water outlet is communicated with the fermentation product collecting tank, and the gas outlet is communicated to the atmosphere; the water separator is provided with a valve for controlling the circulation of gas and the circulation of fermentation liquor.
Furthermore, the invention also claims the application of the oxygen micro-nano bubble enhanced aerobic fermentation bioreactor in the fermentation production of L-lysine by immobilized corynebacterium glutamicum.
Further, the specific steps of the reactor for producing L-lysine by fermentation of immobilized Corynebacterium glutamicum comprise:
(1) firstly, respectively carrying out steam sterilization on materials in a seed tank and a batching tank and immobilized carriers in a fermentation tank for later use;
(2) then, simultaneously introducing the filtered and sterilized oxygen and the activated seed liquid into a micro-nano bubble generator, controlling the flow of the oxygen at 200-250 g/h to form oxygen micro-nano bubbles containing cells, and further introducing the oxygen micro-nano bubbles and the activated seed liquid into a fermentation tank at the flow of 6-8L/min to perform immobilized cell fermentation; wherein the average size of the oxygen micro-nano bubbles is below 220nm, and the existence time is above 630 s; the stirring speed in the fermentation tank is controlled below 180 rpm;
(3) and after the fermentation is finished, circularly separating the fermentation liquor by using the gas-liquid separation tank and the water separator, and finally enabling the product to flow into a fermentation product collecting tank.
Compared with the existing aerobic fermentation bioreactor, the formation of the oxygen micro-nano bubbles can reduce the stirring speed by 10%, improve the L-lysine yield by more than 20%, improve the L-lysine yield by more than 68%, and prolong the existence time by more than 6% after further recycling.
Has the advantages that:
(1) the large bubbles formed by the traditional oxygenation mode show a tendency of gradually rising and becoming large, then break near the liquid level and generate a large amount of foam in fermentation liquor, so that oxygen mass transfer is limited, fermentation products are slowly formed, the fermentation period is obviously prolonged, and meanwhile, the foam rises to the top of a fermentation tank and seeps out from a shaft seal, so that the risk of bacterial contamination is increased. Compared with the prior art, the micro-nano bubbles can be maintained in the fermentation liquor for a long time without cracking until the micro-nano bubbles disappear, so that the mass transfer efficiency is improved, the risk of bacterial contamination in the fermentation process is reduced, and the influence of adding a defoaming agent on the activity and metabolism of the bacterial strain is avoided.
(2) The micro-nano bubble generating device and the immobilized cell fermentation system can achieve the effect of efficient oxygen supply without increasing the stirring speed of the fermentation tank, and the cells are prevented from being damaged by water shearing force and mechanical force due to the fact that the stirring speed is too high.
(3) The recycling of oxygen micro-nano bubbles and good physiological activity can accelerate cell metabolism and improve the growth and proliferation capacity of cells on the one hand, and also can be helpful for the efficient enrichment of nutrient substances on the surface of immobilized cells in a fermentation tank on the other hand, thereby improving the continuous fermentation performance of the immobilized cells and promoting the conversion of products.
(4) In the process that the seed liquid is subjected to primary and secondary air dissolving, the oxygen micro bubbles wrap the microbial cells and enter the nano bubble pump, so that the initial cell activity of the seed liquid is improved to a certain extent, meanwhile, the cell membrane permeability can be improved by proper pressure, the mass transfer performance is improved, and the cell metabolic flux is enhanced along the direction of a target product.
Drawings
The foregoing and/or other advantages of the invention will become further apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings.
FIG. 1 is a schematic diagram of the overall structure of an oxygen micro-nano bubble enhanced aerobic fermentation bioreactor.
Wherein each reference numeral represents:
1. an oxygen generator; 2. a seed tank; 3. a gas filter; 4. a gas-liquid mixing pump; 5. a micro-nano bubble generator; 6. a nano bubble pump; 7. a safety valve; 8. a pressure tank; 9. a bubble atomizer; 10. a water lifting pump; 11. an air pump; 12. a motor; 13. a stirring paddle; 14. a heat exchanger; 15. a first vapor phase equilibrium tube; 16. a dosing tank; 17. a fermentation tank; 18. a water pump; 19. a water tank; 20. a second vapor phase equilibrium tube; 21. a gas-liquid separation tank; 22. a fermentation product collecting tank 23 and a valve; 24. a water separator.
Detailed Description
The invention will be better understood from the following examples.
Example 1:
as shown in figure 1, the oxygen micro-nano bubble enhanced aerobic fermentation bioreactor comprises an oxygen generator 1, a seeding tank 2, a gas-liquid mixing pump 4, a micro-nano bubble generator 5, a fermentation tank 17, a dosing tank 16, a gas-liquid separation tank 21 and a fermentation product collecting tank 22.
Wherein, the gas-liquid mixing pump 4, the micro-nano bubble generator 5, the fermentation tank 17, the gas-liquid separation tank 21 and the fermentation product collecting tank 22 are sequentially connected through pipelines; the dosing tank 16 is connected to a fermentation tank 17.
The oxygen generator 1 and the seed tank 2 are respectively connected to a first inlet and a second inlet of a gas-liquid mixing pump 4, oxygen and seed liquid are sent into the gas-liquid mixing pump 4 to be mixed, then sent into a micro-nano bubble generator 5 to form oxygen micro-nano bubbles through shearing, then sent into a fermentation tank 17 to be fermented together with raw materials sent into a mixing tank 16, and fermented products are separated by a gas-liquid separation tank 21 and then sent into a fermented product collection tank 22.
The micro-nano bubble generator 5 comprises a nano bubble pump 6, a safety valve 7, a pressure tank 8 and a bubble atomizer 9; the feed inlet of the nano bubble pump 6 is connected with the discharge outlet of the gas-liquid mixing pump 4; the nano bubble pump 6, the pressure tank 8 and the bubble atomizer 9 are connected in sequence; the discharge hole of the bubble atomizer 9 is connected with the feed inlet of the fermentation tank 17; the nano bubble pump 6 is connected with the pressure tank 8 through a gas circulating pipe, the excessive oxygen in the pressure tank 8 is returned to the nano bubble pump 6, and the safety valve 7 is installed on the gas circulating pipe.
The discharge hole of the bubble atomizer 9 is connected with the feed inlet below the side surface of the fermentation tank 17, and a water lifting pump 10 is arranged on the connecting pipeline; the gas outlet at the top of the fermentation tank 17 is connected to the pressure tank 8 through a gas circulating pipe, the redundant oxygen in the fermentation tank 17 is returned to the pressure tank 8, and the gas circulating pipe is provided with an air pump 11.
The fermentation tank 17 is a mechanical stirring type fermentation tank, a feed inlet below the side surface of the fermentation tank is connected with the micro-nano bubble generator 5, a feed inlet at the top is connected with the batching tank 16, and a discharge outlet at the bottom is connected with the gas-liquid separation tank 21.
The inside of the fermentation tank 17 is provided with a stirring paddle 13, which is connected with a motor 12 positioned above the top of the fermentation tank 17 and drives the stirring paddle 13 through the motor 12 to stir the materials in the fermentation tank 17. The stirring device can be a propeller stirrer, a vortex stirrer, a paddle stirrer, a shaft stirrer and a helical ribbon stirrer, the stirring speed is controlled to be 150-240 rpm so as to prevent shearing force formed by excessively high stirring speed from causing irreversible damage to cells, and the excessively low stirring speed can cause insufficient dissolved oxygen in the fermentation process.
A cylindrical and hollow silk screen fixer is arranged in the center of the reaction cavity in the fermentation tank 17 and used for fixing cotton fibers; the stirring paddle 13 penetrates through the middle hollow part of the screen fixer. The size of the cotton fiber is 60cm x 120 cm-80 cm x 160cm, and the adding amount is 30-50 g/L.
The fermentation tank 17 is also provided with a heat exchanger 14, and the temperature of the fermentation liquid in the fermentation tank 17 is adjusted through the heat exchanger 14. The heat exchanger can be a jacketed heat exchanger, a U-shaped tube plate heat exchanger, a floating head heat exchanger, a plate heat exchanger and the like, the refrigerant used by the heat exchanger is sterile water, and the water temperature is controlled to be 4-10 ℃.
The heat exchanger 14 is a built-in multi-group coil heat exchanger and is fixed on the inner wall of a reaction cavity of the mechanical stirring fermentation tank, the heat exchanger 14 is connected with a water tank 19 outside the fermentation tank through a water pipe, a water pump 18 is arranged on the water pipe, and pre-cooled sterile water in the water tank 19 is sent into a coil in the fermentation tank through the water pump 18 to exchange heat with fermentation liquor in the tank.
A first gas phase balance pipe 15 is arranged on a pipeline connecting a feed inlet at the top of the fermentation tank 17 with the batching tank 16; the batching tank 16 is used for containing an aseptic fermentation medium which is 4-10 m 3 The flow rate/h is to the reaction chamber via the inlet opening of the fermentation tank 17, in order to balance the pressure in the dosing tank 16 and the fermentation tank 17, a gas phase balance tube is arranged on the connecting line of the two. Fermentation tank17 the pipeline connecting the bottom discharge port with the gas-liquid separation tank 21 is provided with a second gas phase balance pipe 20 to maintain the pressure balance of the gas-liquid separation tank and the mechanical stirring type fermentation tank.
The pipeline connecting the oxygen generator 1 and the gas-liquid mixing pump 4 is provided with a gas filter 3, so that the seed liquid is prevented from being polluted by the mixed bacteria in the oxygen. Controlling the flow rate of oxygen gas to be 200-500 g/h, and controlling the speed of the seed liquid entering the gas-liquid mixing pump after the seed liquid is activated for the second time to be 3-10 m 3 /h。
The oxygen microbubble and seed liquid mixed liquid flows out of a liquid outlet of the gas-liquid mixing pump 4 by 0.5-3 m 3 The liquid flow of/h flows into the nano bubble pump 6, secondary pressurization is carried out in the pressure tank 8 to dissolve gas, in the process, the pressure of the pressure tank 8 is controlled within the range of 0.5-20 MPa, in order to prevent the pressure in the tank from being too high, a safety valve 7 is arranged at an air inlet at the top of the pressure tank, when the pressure in the tank is too high, the safety valve 7 is manually opened, and oxygen in the pressure tank can be circulated to the nano bubble pump 6 through a connecting pipeline for secondary use.
The micro-nano bubbles wrapped with the cells are discharged through an outlet of the bubble atomizer 9, the flow rate is 6-10L/min, and the micro-nano bubbles enter the reaction cavity through a connecting pipeline by an air inlet at the bottom of a shell of the mechanical stirring type fermentation tank 17, so that the micro-nano bubbles enter the fermentation liquor from bottom to top.
The bottom feed inlet of the gas-liquid separation tank 21 is connected with the discharge outlet of the fermentation tank 17, the middle separation feed inlet is connected with the fermentation product collecting tank 22, and the top gas outlet is connected with the water separator 24. The water separator 24 is used for realizing the circular separation of the fermentation product and achieving the flow-dividing and pressure-stabilizing effects of the gas-liquid separation tank and the fermentation product collection tank.
The water separator 24 is provided with a water outlet, an air inlet, an air outlet, a circulating air outlet and a circulating air inlet, wherein the circulating air inlet is communicated with the air outlet at the top of the gas-liquid separation tank 21, the circulating air outlet is communicated with the air inlet in the water separator body, the water outlet is communicated with the fermentation product collecting tank 22, and the air outlet is communicated to the atmosphere; the gas circulation and the fermentation liquid circulation are controlled by valves 23.
Example 2:
adopt above-mentioned device to carry out the fermentation of immobilized corynebacterium glutamicum and produce L-lysine, specifically include:
seed culture and fermentation of corynebacterium glutamicum:
(1) selecting strains for fermentation: corynebacterium glutamicum wild strain KFCC 11043, this laboratory preservation.
(2) The seed culture medium (g/L) is sucrose 20, peptone 10, yeast powder 5, urea 3.5, ammonium sulfate 5, MgSO 5 4 ·7H 2 O 0.5，KH 2 PO 4 4，K 2 HPO 4 10.5, 0.5 of cysteine and 10mL of trace element solution a.
(3) The fermentation medium (g/L) is glucose 80, yeast powder 8, urea 15, ammonium sulfate 13.2, MgSO 4 ·7H 2 O 0.6，K 2 HPO 4 1.5，FeSO 4 0.2, 3-morpholine propanesulfonic acid 42 and trace element solution b 10 mL.
(4) The trace element solution a (mg/L) is calcium D-pantothenate 2, nicotinamide 3, vitamin B 1 1.5 and 0.15 of biotin, and adding the mixture into a seed culture medium after filtering and sterilizing.
(5) The trace element solution B (mg/L) is copper sulfate 0.9, zinc sulfate 1, biotin 1.8, vitamin B 1 9, manganese sulfate 150, D-calcium pantothenate 9 and nicotinamide 60, filtering and sterilizing, and then adding into a fermentation medium.
(6) Activating the seed liquid of the shake flask at 30 ℃ and 220rpm for 12h, transferring the activated seed liquid of the corynebacterium glutamicum to a seed tank 2 at 30 ℃ and culturing at 220rpm until OD 600 Is used for immobilized cell fermentation within the range of 0.3-0.4.
The bioreactor is used for the immobilized fermentation operation of corynebacterium glutamicum and is carried out according to the following flow:
(7) first, a cotton fiber with a size of 70cm x 140cm was wrapped on a cylindrical, hollow wire mesh holder with an addition of 35g/L, placed in the center of the reaction chamber in the fermentation tank 17, and then the materials in the seed tank 2, the blending tank 16, and the fermentation tank 17 were separately steam-sterilized at 115 ℃ for 30min for future use.
(8) Then, after the first activation by shaking the bottle and the second transfer by the seeding tank 2, the OD of the seed liquid is controlled 600 In the range of 0.3 to 0.4Starting the oxygen generator 1 and the seeding tank 2, adjusting the oxygen flow to 240g/h after filtering and sterilizing by the gas filter 3, and simultaneously, the speed of seed liquid entering the gas-liquid mixing pump 4 reaches 5m 3 H, after the action of the gas-liquid mixing pump 4, the oxygen microbubbles are uniformly mixed with the seed liquid to form a mixed liquid with the volume of 1m 3 The flow rate of the nano bubbles per hour is controlled to flow into a nano bubble pump 6, secondary pressurization is carried out by using a pressure tank 8, the pressure is controlled to be 8MPa, then oxygen micro-nano bubbles are discharged by a bubble atomizer 9, at the moment, the oxygen micro-nano bubbles wrapped with corynebacterium glutamicum are formed, the average size of the bubbles is 220nm, and the existence time is more than 650 s.
(9) Further, the motor 12, the mixing tank 16 and the water pump 18 are started, so that the corresponding stirring speed of the stirring paddle 13 reaches 180rpm, and the material in the mixing tank 16 is 6m 3 The flow rate is/h into the reaction chamber of the fermenter 17, during which the first gas phase equalization line 15 is opened for maintaining the pressure equalization of both the dosing tank 16 and the fermenter 17, while the pre-cooled 5 ℃ sterile water in the water tank 19 is acted upon by the water pump 18 for 1m 3 The flow rate/h through the heat exchanger 14 serves to prevent local temperature overshoot leading to reduced cell activity.
(10) And (3) after the temperature of the fermentation liquid in the fermentation tank 17 is stably maintained at 30 ℃, allowing the oxygen micro-nano bubbles containing the cells prepared in the step (8) to flow into the reaction cavity through a feed inlet below the side surface of the fermentation tank 17 at a flow rate of 8L/min for immobilized cell fermentation.
(11) After 20h of fermentation is finished, the second gas phase balance pipe 20 is opened, the fermentation tank 17 and the gas-liquid separation tank 21 are in a pressure balance state, fermentation liquor enters the gas-liquid separation tank 21 from the communicating pipeline and is discharged into the fermentation liquor collection tank 22 through the separation material port, and in addition, in order to prevent the untimely discharge of the excessive fermentation liquor and the loss of entrainment effect of micro-nano bubbles on the fermentation liquor, the product and the gas are further separated by the water separator 24.
(12) The gas in the water separator 24 is continuously circulated, the fermentation liquid is further separated, corresponding gas and liquid valves are opened, finally, the residual fermentation liquid flows into the fermentation product collecting tank 22 through the water outlet, and the gas is discharged into the atmosphere.
(13) And (3) after the fermentation liquid completely flows out and is collected, performing secondary fermentation by using the immobilized cells on the surface of the cotton fiber in the fermentation tank 17 without re-inoculation, wherein the material in the dosing tank 16 is refilled and subjected to steam sterilization for later use, and the specific operation flow is consistent with the operation flows (9) - (12).
In the single batch of immobilized cell fermentation process, the fermentation period is 20h, the L-lysine yield is 38g/L, the L-lysine yield is 1.9g/L/h, the L-lysine yield can reach 47.5%, the size of oxygen micro-nano bubbles is 220nm, and the existence time can reach more than 630 s.
Further, in the continuous immobilized cell fermentation process, the fermentation period is shortened from 20h to 8h, the L-lysine yield is increased to 48g/L at the 8 th time, the L-lysine yield is 6g/L/h and can reach 60%, the size of the oxygen micro-nano bubbles can be reduced to 7-200 nm through multiple cycles, and the existence time can reach 670 s.
Comparative example
The same examples were used for the activation and seed culture of C.glutamicum strains.
The fermentation of the corynebacterium glutamicum immobilized cells was performed according to the method of the example, except that the entire bioreactor system did not contain the micro-nano bubble generator 5, and the bubbles generated by the gas-liquid mixing pump 4 were directly introduced into the reaction chamber from the inlet at the bottom of the fermentor 17.
In contrast, in the single batch of immobilized cell fermentation process, in order to ensure consistent fermentation period, the stirring speed corresponding to the stirring paddle 13 needs to be increased to 200rpm to enhance dissolved oxygen, the yield of L-lysine is 36g/L, the yield of L-lysine is 1.8g/L/h, the yield of L-lysine can reach 45%, the size of oxygen microbubbles is 580nm, and the existence time is 280 s.
Further, in the continuous immobilized cell fermentation process, the fermentation period is shortened from 20h to 12h, the yield of L-lysine is increased to 43g/L at the 10 th time, the yield of L-lysine is 3.58g/L/h, the yield of L-lysine is 53.75%, the size of oxygen micron bubbles can be reduced to 290-560 nm through multiple cycles, and the existence time can reach 300 s.
The invention provides an oxygen micro-nano bubble enhanced aerobic fermentation bioreactor and an application concept and a method thereof, and a plurality of methods and ways for realizing the technical scheme are provided. All the components not specified in the present embodiment can be realized by the prior art.
Claims (10)
1. An oxygen micro-nano bubble enhanced aerobic fermentation bioreactor is characterized by comprising an oxygen generator (1), a seeding tank (2), a gas-liquid mixing pump (4), a micro-nano bubble generator (5), a fermentation tank (17), a batching tank (16), a gas-liquid separation tank (21) and a fermentation product collecting tank (22);
the gas-liquid mixing pump (4), the micro-nano bubble generator (5), the fermentation tank (17), the gas-liquid separation tank (21) and the fermentation product collecting tank (22) are sequentially connected through a pipeline; the batching tank (16) is connected with the fermentation tank (17);
the oxygen generator (1) and the seed tank (2) are respectively connected to a first inlet and a second inlet of the gas-liquid mixing pump (4), oxygen and seed liquid are sent into the gas-liquid mixing pump (4) to be mixed, then sent into the micro-nano bubble generator (5) to form oxygen micro-nano bubbles through shearing, then sent into the fermentation tank (17) to be fermented together with the raw material sent into the dosing tank (16), and fermented products are separated by the gas-liquid separation tank (21) and then sent into the fermented product collection tank (22).
2. The oxygen micro-nano bubble enhanced aerobic fermentation bioreactor according to claim 1, characterized in that the micro-nano bubble generator (5) comprises a nano bubble pump (6), a safety valve (7), a pressure tank (8) and a bubble atomizer (9); the feed inlet of the nano bubble pump (6) is connected with the discharge outlet of the gas-liquid mixing pump (4); the nano bubble pump (6), the pressure tank (8) and the bubble atomizer (9) are connected in sequence; the discharge hole of the bubble atomizer (9) is connected with the feed inlet of the fermentation tank (17); the nano bubble pump (6) is connected with the pressure tank (8) through a gas circulating pipe, excessive oxygen in the pressure tank (8) is returned to the nano bubble pump (6), and the safety valve (7) is installed on the gas circulating pipe.
3. The oxygen micro-nano bubble enhanced aerobic fermentation bioreactor according to claim 2, characterized in that a discharge port of the bubble atomizer (9) is connected with a feed port below the side surface of the fermentation tank (17), and a water lift pump (10) is arranged on a connecting pipeline; and a gas outlet at the top of the fermentation tank (17) is connected to the pressure tank (8) through a gas circulating pipe, so that redundant oxygen in the fermentation tank (17) is returned to the pressure tank (8), and the gas circulating pipe is provided with an air suction pump (11).
4. The oxygen micro-nano bubble enhanced aerobic fermentation bioreactor according to claim 1, wherein the fermentation tank (17) is a mechanical stirring type fermentation tank, a feed inlet below the side surface is connected with the micro-nano bubble generator (5), a feed inlet at the top is connected with a dosing tank (16), and a discharge outlet at the bottom is connected with a gas-liquid separation tank (21);
a stirring paddle (13) is arranged in the fermentation tank (17), and is connected with a motor (12) positioned above the top of the fermentation tank (17) and drives the stirring paddle (13) to stir the materials in the fermentation tank (17) through the motor (12);
a cylindrical hollow silk screen fixer is arranged in the center of the reaction cavity in the fermentation tank (17) and is used for fixing cotton fibers; the stirring paddle (13) penetrates through the hollow part in the middle of the silk screen fixer;
the fermentation tank (17) is also provided with a heat exchanger (14), and the temperature of the fermentation liquid in the fermentation tank (17) is adjusted through the heat exchanger (14).
5. The oxygen micro-nano bubble enhanced aerobic fermentation bioreactor according to claim 4, characterized in that the heat exchanger (14) is a built-in multi-group coil heat exchanger fixed on the inner wall of the reaction cavity of the mechanical stirring fermentation tank, the heat exchanger (14) is connected with a water tank (19) outside the fermentation tank through a water pipe, a water pump (18) is arranged on the water pipe, and the pre-cooled sterile water in the water tank (19) is sent into the coil in the fermentation tank through the water pump (18) to exchange heat with the fermentation liquor in the tank.
6. The oxygen micro-nano bubble enhanced aerobic fermentation bioreactor according to claim 4, characterized in that a first gas phase balance pipe (15) is arranged on a pipeline connecting a feed inlet at the top of the fermentation tank (17) and the dosing tank (16); a second gas phase balance pipe (20) is arranged on a pipeline connecting a discharge hole at the bottom of the fermentation tank (17) and the gas-liquid separation tank (21).
7. The oxygen micro-nano bubble enhanced aerobic fermentation bioreactor according to claim 1, wherein a feed inlet at the bottom of the gas-liquid separation tank (21) is connected with a discharge outlet of the fermentation tank (17), a middle separation feed inlet is connected with a fermentation product collection tank (22), and a gas outlet at the top is connected with a water separator (24).
8. The oxygen micro-nano bubble enhanced aerobic fermentation bioreactor according to claim 7, wherein the water separator (24) is provided with a water outlet, a gas inlet, a gas outlet, a circulating gas outlet and a circulating gas inlet, the circulating gas inlet is communicated with the gas outlet at the top of the gas-liquid separation tank (21), the circulating gas outlet is communicated with the gas inlet in the water separator body, the water outlet is communicated with the fermentation product collection tank (22), and the gas outlet is communicated to the atmosphere; the water separator (24) is provided with a valve (23) for controlling the circulation of gas and the circulation of fermentation liquor.
9. Use of a bioreactor as claimed in any of claims 1 to 8 for the fermentative production of L-lysine in immobilized corynebacterium glutamicum.
10. Use according to claim 9, characterized in that it comprises the following steps:
(1) respectively carrying out steam sterilization on the materials in the seeding tank (2) and the proportioning tank (16) and the immobilized carrier in the fermentation tank (17) for later use;
(2) simultaneously introducing the filtered and sterilized oxygen and the activated seed liquid into a micro-nano bubble generator (5), controlling the flow of the oxygen at 200-250 g/h to form oxygen micro-nano bubbles containing cells, and further introducing the oxygen micro-nano bubbles and the activated seed liquid into a fermentation tank (17) at the flow of 6-8L/min to perform immobilized cell fermentation; wherein the average size of the oxygen micro-nano bubbles is below 220nm, and the existence time is above 630 s; the stirring speed in the fermentation tank (17) is controlled below 180 rpm;
(3) after fermentation is finished, the fermentation liquid is circularly separated by using the gas-liquid separation tank (21) and the water separator (24), and the product finally flows into the fermentation product collecting tank (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210670062.8A CN114806810A (en) | 2022-06-14 | 2022-06-14 | Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210670062.8A CN114806810A (en) | 2022-06-14 | 2022-06-14 | Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114806810A true CN114806810A (en) | 2022-07-29 |
Family
ID=82520710
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210670062.8A Pending CN114806810A (en) | 2022-06-14 | 2022-06-14 | Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114806810A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115141748A (en) * | 2022-06-14 | 2022-10-04 | 南京工业大学 | Microporous membrane tube reactor and application thereof in immobilized enzyme catalytic reaction |
| CN116396855A (en) * | 2023-05-15 | 2023-07-07 | 南京工业大学 | Biological fermentation reactor and method based on jet flow rotary stirring |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080264843A1 (en) * | 2006-09-25 | 2008-10-30 | Sharp Kabushiki Kaisha | Manufacturing method, manufacturing device, and application device for liquid containing micro-nano bubbles |
| RU2378358C1 (en) * | 2008-07-15 | 2010-01-10 | Николай Аполлонович Мехузла | Method of processing grapes and grapes processing line |
| JP2011088076A (en) * | 2009-10-22 | 2011-05-06 | Panasonic Electric Works Co Ltd | Method and apparatus for generating gas-liquid mixed liquid |
| CN102350256A (en) * | 2011-07-01 | 2012-02-15 | 南阳启伟微生态基因科技开发有限公司 | Micro-nano bubble generating device, and fermentation apparatus and fermentation method using same |
| CN103555560A (en) * | 2013-10-28 | 2014-02-05 | 南京工业大学 | Device and method for preparing butanol by acetone-butanol fermentation coupling separation and purification |
| WO2014060778A1 (en) * | 2012-10-19 | 2014-04-24 | Advanced Technology And Engineering Limited (Atel) | Fermentation apparatus and method of fermentation for protein production |
| CN107904142A (en) * | 2017-12-29 | 2018-04-13 | 无锡海悦生化装备有限公司 | Fermentation tank gas-liquid separator |
| CN207641271U (en) * | 2017-10-13 | 2018-07-24 | 南京意西欧环境科技有限公司 | Vertical exhaust treatment system based on dynamic interception and micro-nano bubbler techniques |
| CN112169720A (en) * | 2019-07-04 | 2021-01-05 | 南京延长反应技术研究院有限公司 | Nano-micro interface enhanced reaction system |
| CN112481073A (en) * | 2019-09-11 | 2021-03-12 | 南京延长反应技术研究院有限公司 | Micro-interface enhanced fermentation system and process |
| CN113862134A (en) * | 2021-10-09 | 2021-12-31 | 沈阳航空航天大学 | Micro-nano bubble circulation hydrogen supply anaerobic fermentation tank |
| CN217459384U (en) * | 2022-06-14 | 2022-09-20 | 南京工业大学 | Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor |
-
2022
- 2022-06-14 CN CN202210670062.8A patent/CN114806810A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080264843A1 (en) * | 2006-09-25 | 2008-10-30 | Sharp Kabushiki Kaisha | Manufacturing method, manufacturing device, and application device for liquid containing micro-nano bubbles |
| RU2378358C1 (en) * | 2008-07-15 | 2010-01-10 | Николай Аполлонович Мехузла | Method of processing grapes and grapes processing line |
| JP2011088076A (en) * | 2009-10-22 | 2011-05-06 | Panasonic Electric Works Co Ltd | Method and apparatus for generating gas-liquid mixed liquid |
| CN102350256A (en) * | 2011-07-01 | 2012-02-15 | 南阳启伟微生态基因科技开发有限公司 | Micro-nano bubble generating device, and fermentation apparatus and fermentation method using same |
| WO2014060778A1 (en) * | 2012-10-19 | 2014-04-24 | Advanced Technology And Engineering Limited (Atel) | Fermentation apparatus and method of fermentation for protein production |
| CN103555560A (en) * | 2013-10-28 | 2014-02-05 | 南京工业大学 | Device and method for preparing butanol by acetone-butanol fermentation coupling separation and purification |
| CN207641271U (en) * | 2017-10-13 | 2018-07-24 | 南京意西欧环境科技有限公司 | Vertical exhaust treatment system based on dynamic interception and micro-nano bubbler techniques |
| CN107904142A (en) * | 2017-12-29 | 2018-04-13 | 无锡海悦生化装备有限公司 | Fermentation tank gas-liquid separator |
| CN112169720A (en) * | 2019-07-04 | 2021-01-05 | 南京延长反应技术研究院有限公司 | Nano-micro interface enhanced reaction system |
| CN112481073A (en) * | 2019-09-11 | 2021-03-12 | 南京延长反应技术研究院有限公司 | Micro-interface enhanced fermentation system and process |
| CN113862134A (en) * | 2021-10-09 | 2021-12-31 | 沈阳航空航天大学 | Micro-nano bubble circulation hydrogen supply anaerobic fermentation tank |
| CN217459384U (en) * | 2022-06-14 | 2022-09-20 | 南京工业大学 | Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115141748A (en) * | 2022-06-14 | 2022-10-04 | 南京工业大学 | Microporous membrane tube reactor and application thereof in immobilized enzyme catalytic reaction |
| CN116396855A (en) * | 2023-05-15 | 2023-07-07 | 南京工业大学 | Biological fermentation reactor and method based on jet flow rotary stirring |
| CN116396855B (en) * | 2023-05-15 | 2024-05-07 | 南京工业大学 | Biological fermentation reactor and method based on jet flow rotary stirring |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN217459384U (en) | Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor | |
| AU2018200574B2 (en) | Method for producing compound and compound production system used in said production method | |
| CN114806810A (en) | Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor and application thereof | |
| CN102350256B (en) | Micro-nano bubble generating device, and fermentation apparatus and fermentation method using same | |
| CN101312795A (en) | Hydrogen fermentor and method of producing hydrogen | |
| CN102311924B (en) | Method for open-type culture of microalgae | |
| JP2022534726A (en) | Microbial growth bioreactor | |
| US3969190A (en) | Apparatus and method for microbial fermentation in a zero gravity environment | |
| US20100291621A1 (en) | Anaerobic process | |
| Tango et al. | A continuous lactic acid production system using an immobilized packed bed of Lactobacillus helveticus | |
| CN102127505B (en) | Immobilized cell bioreactor | |
| CN101153294B (en) | Immobilized cell single-tank high-strength continuous fermentation process for succinic acid | |
| CN114854536B (en) | Micro-interface enhanced bioreactor for synthesis gas conversion and application thereof | |
| CN107487860A (en) | A kind of fish-farming with sewage microalgae device and preparation method thereof | |
| CN109735452A (en) | Airlift bioreactor for membrane tube gas distribution and application thereof | |
| CN217578866U (en) | Micro-interface enhanced high-efficiency bioreactor for synthesis gas conversion | |
| CN114350480B (en) | Double-circulation bubbling fermentation tank and method for preparing lactic acid by fermentation | |
| JPH0568235B2 (en) | ||
| CN214193258U (en) | Raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate | |
| CN104531506B (en) | Fiber bed reactor suitable for aerobic fermentation and method for producing L-ornithine by using fiber bed reactor | |
| CN114591878A (en) | Process for preparing mariculture tail water treatment microbial inoculum and tail water treatment method | |
| JPH02138960A (en) | Liquid flow type biochemical reactor | |
| CN1498959A (en) | Method and device of solid-state fermentation by using porous adsorption carrier | |
| CN101870946A (en) | Bioreactor suitable for fermentation of long-chain polyunsaturated fatty acid | |
| CN201704321U (en) | Bioreactor suitable for fermentation of long-chain polyunsaturated fatty acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |