CN1498959A - Method and device of solid-state fermentation by using porous adsorption carrier - Google Patents
Method and device of solid-state fermentation by using porous adsorption carrier Download PDFInfo
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- CN1498959A CN1498959A CNA021467633A CN02146763A CN1498959A CN 1498959 A CN1498959 A CN 1498959A CN A021467633 A CNA021467633 A CN A021467633A CN 02146763 A CN02146763 A CN 02146763A CN 1498959 A CN1498959 A CN 1498959A
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- absorption carrier
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Abstract
A solid-state fermenting method and apparatus with porous adsorption carrier features that on the basis of traditional horizontal solid-state fermenting method and apparatus, a porous adsorption carrier and its cylindrical container are used for the culture medium, and after the fermentation is finished, a counter-current extracting process is used to separate product. Its advantages are high fermenting level, and use of aseptic inoculation and ultrasonic moisture control.
Description
Technical field
The invention belongs to the fermentation industry technical field, particularly a kind of porous absorption carrier solid-state fermentation technology and device thereof.
Background technology
Solid state fermentation is one of human use's microorganisms producing product technology with the longest history, before several thousand just by the mankind in order to make various traditional foods.In recent years, solid state fermentation was developed rapidly and was used in the research in fields such as organic acid, alcohol, microbiotic, gene recombination bacterium fermentation medicine, and solid state fermentation is used has huge potential.
Solid state fermentation is meant that generally substratum is solid-state, does not have or almost do not have one or more fermentation process that carry out under the state of free water.Solid state fermentation has following characteristics: (1) can directly utilize thick agricultural-food raw material (grain and cellulose raw material) etc. to be substrate, raw material cheapness; (2) simulating nature growing environment makes microorganism keep the growth conditions similar to nature; (3) no a large amount of organic waste water produces, and belongs to process for cleanly preparing; (4) tunning is adsorbed on (thick agricultural-food raw material) in the fermentation residuum, the aftertreatment complexity, and the high product of purity requirement is unfavorable for fermenting.Enhancing along with people's environmental protection consciousness; everybody begins to pay close attention to this process for cleanly preparing of solid state fermentation; but traditional solid state fermentation only is applicable to the leavened prod production low to purity requirement; owing to adopt the coarse fodder fermentation; be difficult to be applied to the high leavened prod of purity requirement, limited the development of solid state fermentation greatly.
Summary of the invention
The objective of the invention is to be applied to the high problems such as leavened prod of purity requirement, and a kind of porous absorption carrier process for solid state fermentation is provided in order to solve being difficult to that present solid-state fermenter exists.
Another object of the present invention is for a kind of porous absorption carrier solid-state fermentation installation, this device are provided.
Purpose of the present invention can realize by following measure:
A kind of porous absorption carrier process for solid state fermentation comprises the steps:
(1) the porous absorption carrier is contained in the fermenting container, and on the porous absorption carrier, is 1: 1.5-2.5 absorption solubility substratum by the solid-liquid volume ratio;
(2) inoculation fermentation bacterium in the porous absorption carrier, zymogenic weight is the 2-10% of the weight of substratum;
(3) temperature 25--35 ℃ in the control porous support, humidity 85%--95% ferments;
(4) after the fermentation ends, enter purification procedures.
Step also comprises a sterilization process in above-mentioned (1), is about to be adsorbed with the porous absorption carrier sterilization of wanting the dissolubility substratum.
Also be included in the solid ferment process after above-mentioned (3) step, for control fermentation humidity, feed sterile air in fermenting container, its air flow is 0.8-1.2vvm.
Described porous absorption carrier is selected from least a in sponge, paper pulp, vermiculite, the porous inert material of zeolites.
Separation and purification in above-mentioned (4) step comprises the steps: its porous support and tunning are poured into respectively in three extracting tanks; At first in the 1st extractor, add extracting solution, the weight ratio of extracting solution and porous absorption carrier is (10-20): 1 times, stirring and leaching 1 hour, then, its vat liquor is pressed into the 2nd extractor lixiviate, successively, to the 3rd extractor lixiviate, again vat liquor is made with extra care variant production by traditional separation purification method.
Another object of the present invention can realize by following measure:
A kind of porous absorption carrier solid-state fermentation installation, this device comprises seed fermentation jar, recirculation blower, solid state fermentation sealed can, wherein the seed fermentation jar links to each other with the solid state fermentation sealed can; It is characterized in that in the solid state fermentation sealed can, being provided with porous absorption carrier tube, and be gas circulation channel between solid state fermentation sealed can and the porous absorption carrier tube; In porous absorption carrier tube, be placed with the porous absorption carrier; Two ends at porous absorption carrier tube are provided with gas distribution grid, are provided with the gas distribution ring before an end gas distribution grid of porous absorption carrier tube.
Before described gas distribution ring, establish recirculation blower.
The diameter of described gas distribution ring is the 1/2-2/3 of gas distribution board diameter, and percentage of open area is 0.2-0.5 on the gas distribution ring.
The diameter of described gas distribution grid is the 1/2-8/10 of solid state fermentation sealed can diameter, and the percentage of open area on the gas distribution grid is 0.5-0.7.
Described porous absorption carrier is selected from a kind of in sponge, paper pulp, vermiculite, the porous inert material of zeolites.
The present invention has following advantage compared to existing technology:
1, the present invention selects porous absorption carriers such as sponge, paper pulp, vermiculite, zeolite for use, and absorption solubility substratum has avoided the impurity in the solids to bring in the fermented extracted liquid so fully, and simultaneously, the porous absorption carrier can be recycled, and reduces production costs greatly.
2, method of the present invention is the simulating nature growing environment, makes microorganism keep the growth conditions similar to nature, and temperature in the fermenting process, humidity, pH are controlled.
3, the present invention is because porous absorption carrier solid state fermentation under no free-water state, being beneficial to gas-liquid transmits admittedly, satisfy the needed oxygen of microbial fermentation and the carbonic acid gas of fermentation generation, the discharge of heat easily, avoid being difficult in the immobilized cell fermentation process problem of oxygen supply and carbonic acid gas discharge fully, fermentation level improves 3--5 doubly than liquid fermenting, and facility investment is more much lower than liquid submerged fermentation.
4, the present invention adopts three stage countercurrent extraction processes, and consumption that can limited control extracting solution is avoided the generation of a large amount of organic waste waters.
Supporting aseptic technique methods such as 5, the present invention adopts that substratum real disappears, liquid seeds aseptic inoculation on the throne and ultrasonic control on the throne are wet, solved that extensive solid medium is difficult to sterilize and the problem of extensive aseptic inoculation, realized proper purebred cultivation.
Description of drawings
Fig. 1 is a process flow sheet of the present invention
1-seed fermentation jar 2-Wen inoculated tube 3-ultrasonic atomizer 4-recirculation blower
5-solid state fermentation sealed can 6-gas distribution grid 7-porous absorption carrier tube
8-gas circulation channel 9-solid state fermentation cover door 10-valve 11-gas distribution ring
Fig. 2 is solid-state fermenter of the present invention square section and gas distribution ring structure synoptic diagram
Concrete embodiment
With reference to Fig. 1, be process flow system figure of the present invention.The present invention is that the device of the porous absorption carrier solid state fermentation of employing comes fermentative production microbiotic, gene engineering recombinant bacterium proteolytic enzyme and high-density lipoprotein (HDL) medicine etc.
Further specify device of the present invention below in conjunction with Fig. 1,2.This device comprises liquid seeds fermentor tank 1, Wen's inoculated tube 2, ultrasonic atomizer 3, recirculation blower 4, solid state fermentation sealed can 5, gas distribution grid 6, porous absorption carrier tube 7, gas circulation channel 8, solid state fermentation cover door 9, valve 10, gas distribution ring 11 and porous absorption carrier support 12; Place the porous absorption carrier tube 7 that the porous absorption carrier is housed on the support 12 in horizontal solid state fermentation sealed can 5, be provided with gas distribution grid 6 at the two ends of porous absorption carrier tube 7; The diameter of this gas distribution grid 6 is 8/10 of solid-state fermenter 5 diameters, the percentage of open area 0.5-07 on the gas distribution grid 6; At horizontal solid state fermentation sealed can 5 one ends recirculation blower 4 and the gas distribution ring 11 relative with gas distribution grid 6 are installed, the diameter of this gas distribution ring 11 is 2/3 of gas distribution grid 6 diameters, and percentage of open area is 0.2-0.5 on the gas distribution ring 11; The other end of solid state fermentation sealed can 5 is the quick-actuating closure door 9 of turnover porous absorption carrier tube 7; In horizontal solid state fermentation sealed shell of tank 5, make jar intravital gas by forming circulation in the porous absorption carrier inner core 7 and between the gas circulation channel 8 by recirculation blower 4; In horizontal solid state fermentation sealed shell of tank 5 outsides, seed liquor in the liquid seeds fermentor tank 1 is inoculated on the porous absorption carrier by Wen's inoculated tube 2; According to humidity conditions in the solid-state fermenter, open the intravital certain humidity of ultrasonic atomizer 3 controlling tank.Wherein percentage of open area is meant that on plate institute's perforate accounts for the ratio of the total area.
Porous absorption carrier process for solid state fermentation of the present invention is:
(1) pack in the porous absorption carrier tube 7 in horizontal solid state fermentation sealed shell of tank 5 porous absorption carrier and solubility substratum thereof such as sponge, paper pulp, zeolite and vermiculite, wherein the solid-to-liquid ratio of porous absorption carrier and substratum is 1: 2, be warmed up to 120 ℃ of sterilizations 20-30 minute, ON cycle blower fan 4, after substratum is cooled to 40 ℃, by the seed liquor (with respect to the seed liquor of substratum weight 5%) in the Venturi meter 2 inoculation liquid seeding tanks 1 in inserting the porous absorption carrier, 30 ℃ of temperature in the control porous support, humidity 85%--95% ferments;
(2) in solid ferment process, by certain humidity in the ultrasonic atomizer control tank body, entering jar intravital sterile air air flow is 0.8-1.2vvm (air capacity of per minute feeding tank body is 0.8-1.2 a times of tank body volume);
(3) behind the fermentation termination, open tank body quick-actuating closure door 9, take out porous absorption carrier inner core 7, its porous support and tunning are poured into respectively in three extracting tanks; The extracting solution that at first adds 10 times is in the 1st extractor, and stirring and leaching 1 hour then, is pressed into the 2nd extractor lixiviate with its vat liquor, successively, to the 3rd extractor lixiviate, again vat liquor is made with extra care variant production by traditional separation purification method;
Embodiment 1: porous absorption carrier solid-state fermentation technology clavulanic acid
Pack in the porous absorption carrier tube 7 in horizontal solid state fermentation sealed shell of tank 5 paper pulp porous absorption carrier and solubility substratum thereof (solid-to-liquid ratio is 1: 2.5), be warmed up to 120-125 ℃ of sterilization 20-30 minute, ON cycle blower fan 4, after substratum is cooled to 40 ℃, by the seed liquor (with respect to the seed liquor of substratum weight 5%) in the Venturi meter 2 inoculation liquid seeding tanks in inserting the porous absorption carrier, control temperature 25--28 ℃ in the porous support, humidity 85%--95% ferments; In solid ferment process, by certain humidity in the ultrasonic atomizer control tank body, entering jar intravital sterile air air flow is 1.0vvm (air capacity of per minute feeding tank body is 1.0 times of tank body volume); Behind the fermentation termination, open tank body quick-actuating closure door 9, take out porous absorption carrier inner core 7, its porous support and tunning are poured into respectively in three extracting tanks; The extracting solution that at first adds 10 times is in the 1st extractor, and stirring and leaching 1 hour then, is pressed into the 2nd extractor lixiviate with its vat liquor, successively, to the 3rd extractor lixiviate, again vat liquor is made with extra care variant production by traditional separation purification method;
Bacterial classification: carat dimension streptomycete (Streptomyces clavuligerus);
Porous absorption carrier: wood pulp;
Substratum: soy peptone 0.5%, glycerine 0.8%, KH
2PO
40.1%, FeSO
4.7H
2O0.001%, LiCl 0.8%, pH7.0;
Carrier (solid phase) compares with substratum (liquid phase): 1: 2;
Leavening temperature: 25--28 ℃
Liquid inoculation amount: 10% (with the solid phase ratio);
Fermentation period: 4-5 days
Fermentation titer: 120-150r/g (solid phase);
Fermentation vat liquor purity: 45-53%.
Embodiment 2: porous absorption carrier solid-state fermentation technology genetic engineering bacterium Sumizyme MP
Its operating process is with embodiment 1;
Bacterial classification: Bacillus subtilus (Bacillus subtilis PG1723-3);
Porous absorption carrier: sponge;
Substratum: Zulkovsky starch 0.8%, Tryptones 0.2%, lactose 0.2%, kantlex 0.005%, calcium chloride 0.02%, pH10.5;
Carrier (solid phase) compares with substratum (liquid phase): 1: 1.5;
Leavening temperature: 28--32 ℃
Liquid inoculation amount: 10% (with the solid phase ratio);
Fermentation period: 3--4 days
Fermentation titer: 12160-12350IU/g (solid phase);
Fermentation vat liquor purity: 45--55%.
Embodiment 3: porous absorption carrier solid-state fermentation technology genetic engineering bacterium high-density lipoprotein (HDL) medicine
Its operating process is with embodiment 1;
Bacterial classification: pichia pastoris phaff bacterium (Pichia pastoris YAC);
Porous absorption carrier: sponge or vermiculite;
Substratum: methyl alcohol 0.8%, glycerine 12.5%, oleic acid 0.1%, Histidine 0.1%, ammonium sulfate 1.0%, pH5.5;
Carrier (solid phase) compares with substratum (liquid phase): 1: 1--1.5;
Leavening temperature: 25--27 ℃
Liquid inoculation amount: 10% (with the solid phase ratio);
Fermentation period: 3--4 days
Fermentation titer: 1.12g/g (solid phase);
Fermentation vat liquor purity: 45--50%.
Claims (10)
1, a kind of porous absorption carrier process for solid state fermentation comprises the steps:
(1) the porous absorption carrier is contained in the fermenting container, and on the porous absorption carrier, is 1: 1.5-2.5 absorption solubility substratum by the solid-liquid volume ratio;
(2) inoculation fermentation bacterium in the porous absorption carrier, zymogenic weight is the 2-10% of the weight of substratum;
(3) temperature 25--35 ℃ in the control porous support, humidity 85%--95% ferments;
(4) after the fermentation ends, enter purification procedures.
2, porous absorption carrier process for solid state fermentation as claimed in claim 1 is characterized in that also comprising a sterilization process in above-mentioned (1) step, is about to be adsorbed with the porous absorption carrier sterilization of wanting the dissolubility substratum.
3, porous absorption carrier process for solid state fermentation as claimed in claim 1 is characterized in that also being included in the solid ferment process after above-mentioned (3) step, for control fermentation humidity, feeds sterile air in fermenting container, and its air flow is 0.8-1.2vvm.
4, porous absorption carrier process for solid state fermentation as claimed in claim 1 is characterized in that described porous absorption carrier is selected from least a in sponge, paper pulp, vermiculite, the porous inert material of zeolites.
5, porous absorption carrier process for solid state fermentation as claimed in claim 1 is characterized in that the separation and purification in above-mentioned (4) step comprises the steps: its porous support and tunning are poured into respectively in three extracting tanks; At first in the 1st extractor, press and add extracting solution, the weight ratio of extracting solution and porous absorption carrier is (10-20): 1, stirring and leaching 1-2 hour, then, its vat liquor is pressed into the 2nd extractor lixiviate, successively, to the 3rd extractor lixiviate, again vat liquor is made with extra care variant production by traditional separation purification method.
6, a kind of device that is applied to the described porous absorption carrier of claim 1 process for solid state fermentation, this device comprises recirculation blower (4), solid state fermentation sealed can (5); It is characterized in that in solid state fermentation sealed can (5), being provided with porous absorption carrier tube (7), and be gas circulation channel (8) between solid state fermentation sealed can (5) and the porous absorption carrier tube (7); In porous absorption carrier tube (7), be placed with the porous absorption carrier; Be provided with gas distribution grid (6) at the two ends of porous absorption carrier tube (7), at the preceding gas distribution ring (11) that is provided with of an end gas distribution grid (6) of porous absorption carrier tube (7).
7, porous absorption carrier solid-state fermentation installation as claimed in claim 6 is characterized in that at the preceding recirculation blower (4) of establishing of described gas distribution ring (11).
8, porous absorption carrier solid-state fermentation installation as claimed in claim 6, the diameter that it is characterized in that described gas distribution ring (11) is the 1/2-2/3 of gas distribution grid (6) diameter, it is 0.2-0.5 that gas distribution ring (11) is gone up percentage of open area.
9, porous absorption carrier solid-state fermentation installation as claimed in claim 6, the diameter that it is characterized in that gas distribution grid (6) is the 1/2-8/10 of solid state fermentation sealed can (5) diameter, the percentage of open area on the gas distribution grid (6) is 0.5-07.
10, porous absorption carrier solid-state fermentation installation as claimed in claim 6 is characterized in that described porous absorption carrier is selected from a kind of in sponge, paper pulp, vermiculite, the porous inert material of zeolites.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02146763 CN1207391C (en) | 2002-11-06 | 2002-11-06 | Method and device of solid-state fermentation by using porous adsorption carrier |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02146763 CN1207391C (en) | 2002-11-06 | 2002-11-06 | Method and device of solid-state fermentation by using porous adsorption carrier |
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| CN1498959A true CN1498959A (en) | 2004-05-26 |
| CN1207391C CN1207391C (en) | 2005-06-22 |
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| CN 02146763 Expired - Fee Related CN1207391C (en) | 2002-11-06 | 2002-11-06 | Method and device of solid-state fermentation by using porous adsorption carrier |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100335608C (en) * | 2004-12-21 | 2007-09-05 | 中国科学院过程工程研究所 | Inert carrier type equipment and method for solid continuous ferment |
| CN102102081A (en) * | 2009-12-18 | 2011-06-22 | 中国科学院过程工程研究所 | Pure-culture solid-state fermentation apparatus capable of gas-phase inoculation and feeding, and method thereof |
| CN102140445A (en) * | 2010-12-31 | 2011-08-03 | 浙江海洋学院 | Carrier cell immobilization method |
| CN102643870A (en) * | 2012-05-14 | 2012-08-22 | 中国科学院过程工程研究所 | Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation |
| CN102660885A (en) * | 2012-05-14 | 2012-09-12 | 中国科学院过程工程研究所 | Method for preparing high-performance long fiber by utilizing lignocelluloses biological method |
| CN102660430A (en) * | 2012-05-02 | 2012-09-12 | 江苏今世缘酒业股份有限公司 | Air inlet-outlet system for wine-making and koji-making cultivation bed |
-
2002
- 2002-11-06 CN CN 02146763 patent/CN1207391C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100335608C (en) * | 2004-12-21 | 2007-09-05 | 中国科学院过程工程研究所 | Inert carrier type equipment and method for solid continuous ferment |
| CN102102081A (en) * | 2009-12-18 | 2011-06-22 | 中国科学院过程工程研究所 | Pure-culture solid-state fermentation apparatus capable of gas-phase inoculation and feeding, and method thereof |
| CN102140445A (en) * | 2010-12-31 | 2011-08-03 | 浙江海洋学院 | Carrier cell immobilization method |
| CN102140445B (en) * | 2010-12-31 | 2014-09-03 | 浙江海洋学院 | Carrier cell immobilization method |
| CN102660430A (en) * | 2012-05-02 | 2012-09-12 | 江苏今世缘酒业股份有限公司 | Air inlet-outlet system for wine-making and koji-making cultivation bed |
| CN102660430B (en) * | 2012-05-02 | 2013-08-14 | 江苏今世缘酒业股份有限公司 | Air inlet-outlet system for wine-making and koji-making cultivation bed |
| CN102643870A (en) * | 2012-05-14 | 2012-08-22 | 中国科学院过程工程研究所 | Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation |
| CN102660885A (en) * | 2012-05-14 | 2012-09-12 | 中国科学院过程工程研究所 | Method for preparing high-performance long fiber by utilizing lignocelluloses biological method |
| CN102643870B (en) * | 2012-05-14 | 2014-04-30 | 中国科学院过程工程研究所 | Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation |
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| CN1207391C (en) | 2005-06-22 |
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