CN2587875Y - Porous adsorption carrier solid fermentation device - Google Patents
Porous adsorption carrier solid fermentation device Download PDFInfo
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- CN2587875Y CN2587875Y CN 02285096 CN02285096U CN2587875Y CN 2587875 Y CN2587875 Y CN 2587875Y CN 02285096 CN02285096 CN 02285096 CN 02285096 U CN02285096 U CN 02285096U CN 2587875 Y CN2587875 Y CN 2587875Y
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- solid
- absorption carrier
- state fermentation
- porous absorption
- porous
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Abstract
The utility model relates to a porous adsorption carrier solid fermentation device, which comprises a circulation fan, and a solid-state fermentation sealed container which is provided with a porous adsorption carrier tube inside, and gas circulation channel is formed between the solid-state fermentation sealed container and the porous adsorption carrier tube. A porous adsorption carrier is positioned in the porous adsorption carrier tube, both ends of which are provided with gas distributing boards. A gas distributing ring is arranged at front of the gas distributing board on one end of the porous adsorption carrier tube, and the circulation fan is arranged at front of the gas distributing ring. The diameter of the gas distributing ring is half to one third as large as the diameter of each gas distributing board, and the aperture ratio of the gas distributing ring is 0.2 to 0.5. The diameter of each gas distributing board is half to eight tenths as large as the diameter of the solid-state fermentation sealed container. The aperture ratio of each gas distributing board is 0.5 to 0.7. The porous adsorption carrier adopts a porous inert material, such as sponges, paper pulp, vermiculites and zeolites. The utility model can solve the problem that a large-scale solid culture medium is difficult to sterilize and inoculate without asepsis on a large scale, broadens the application range of solid fermentation, and has wide applicability and popularization significance.
Description
Technical field
The utility model belongs to the fermentation industry technical field, particularly a kind of porous absorption carrier solid-state fermentation installation.
Background technology
Solid state fermentation is one of human use's microorganisms producing product technology with the longest history, before several thousand just by the mankind in order to make various traditional foods.In recent years, solid state fermentation was developed rapidly and was used in the research in fields such as organic acid, alcohol, microbiotic, gene recombination bacterium fermentation medicine, and solid state fermentation is used has huge potential.
Solid state fermentation is meant that generally substratum is solid-state, does not have or almost do not have one or more fermentation process that carry out under the state of free water.Solid state fermentation has following characteristics: (1) can directly utilize thick agricultural-food raw material (grain and cellulose raw material) etc. to be substrate, raw material cheapness; (2) simulating nature growing environment makes microorganism keep the growth conditions similar to nature; (3) no a large amount of organic waste water produces, and belongs to process for cleanly preparing; (4) tunning is adsorbed on (thick agricultural-food raw material) in the fermentation residuum, the aftertreatment complexity, and the high product of purity requirement is unfavorable for fermenting.Enhancing along with people's environmental protection consciousness; everybody begins to pay close attention to this process for cleanly preparing of solid state fermentation; but traditional solid state fermentation only is applicable to the leavened prod production low to purity requirement; owing to adopt the coarse fodder fermentation; be difficult to be applied to the high leavened prod of purity requirement, limited the development of solid state fermentation greatly.
Summary of the invention
The purpose of this utility model is to be applied to the high problems such as leavened prod of purity requirement in order to solve being difficult to that present solid-state fermenter exists, and a kind of porous absorption carrier solid-state fermentation installation is provided.
The technical solution of the utility model is as follows:
The porous absorption carrier solid-state fermentation installation that the utility model provides comprises seed fermentation jar, recirculation blower, solid state fermentation sealed can, and wherein the seed fermentation jar links to each other with the solid state fermentation sealed can; It is characterized in that in the solid state fermentation sealed can, being provided with porous absorption carrier tube, and be gas circulation channel between solid state fermentation sealed can and the porous absorption carrier tube; In porous absorption carrier tube, be placed with the porous absorption carrier; Two ends at porous absorption carrier tube are provided with gas distribution grid, are provided with the gas distribution ring before an end gas distribution grid of porous absorption carrier tube;
Before described gas distribution ring, establish recirculation blower.
The diameter of described gas distribution ring is the 1/2-2/3 of gas distribution board diameter, and percentage of open area is 0.2-0.5 on the gas distribution ring.
The diameter of described gas distribution grid is the 1/2-8/10 of solid state fermentation sealed can diameter, and the percentage of open area on the gas distribution grid is 0.5-07.
Described porous absorption carrier is selected from a kind of in sponge, paper pulp, vermiculite, the porous inert material of zeolites.
The utility model has following advantage compared to existing technology:
1, the utility model is selected porous absorption carriers such as sponge, paper pulp, vermiculite, zeolite for use, absorption solubility substratum has avoided the impurity in the solids to bring in the fermented extracted liquid, simultaneously so fully, the porous absorption carrier can be recycled, and reduces production costs greatly.
2, the utility model simulating nature growing environment makes microorganism keep the growth conditions similar to nature, and temperature in the fermenting process, humidity, pH are controlled.
3, the utility model is because porous absorption carrier solid state fermentation under no free-water state, being beneficial to gas-liquid transmits admittedly, satisfy the needed oxygen of microbial fermentation and the carbonic acid gas of fermentation generation, the discharge of heat easily, avoid being difficult in the immobilized cell fermentation process problem of oxygen supply and carbonic acid gas discharge fully, fermentation level improves 3--5 doubly than liquid fermenting, and facility investment is more much lower than liquid submerged fermentation.
4, the utility model adopts three stage countercurrents to extract, and consumption that can limited control extracting solution is avoided the generation of a large amount of organic waste waters.
Supporting aseptic techniques such as 5, the utility model adopts that substratum disappears in fact, liquid seeds aseptic inoculation on the throne and ultrasonic control on the throne are wet, solved that extensive solid medium is difficult to sterilize and the problem of extensive aseptic inoculation, realized proper purebred cultivation.
Description of drawings
Fig. 1 is a structural representation of the present utility model;
1-seed fermentation jar 2-Wen inoculated tube 3-ultrasonic atomizer 4-recirculation blower
5-solid state fermentation sealed can 6-gas distribution grid 7-porous absorption carrier tube
8-gas circulation channel 9-solid state fermentation cover door 10-valve 11-gas distribution ring
Fig. 2 is solid-state fermenter of the present utility model square section and gas distribution ring structure synoptic diagram;
Concrete embodiment
With reference to Fig. 1, the utility model is that the device of the porous absorption carrier solid state fermentation of employing comes fermentative production microbiotic, gene engineering recombinant bacterium proteolytic enzyme and high-density lipoprotein (HDL) medicine etc.
Further specify device of the present utility model below in conjunction with Fig. 1,2.This device comprises liquid seeds fermentor tank 1, Wen's inoculated tube 2, ultrasonic atomizer 3, recirculation blower 4, solid state fermentation sealed can 5, gas distribution grid 6, porous absorption carrier tube 7, gas circulation channel 8, solid state fermentation cover door 9, valve 10, gas distribution ring 11 and porous absorption carrier support 12; Place the porous absorption carrier tube 7 that the porous absorption carrier is housed on the support 12 in horizontal solid state fermentation sealed can 5, be provided with gas distribution grid 6 at the two ends of porous absorption carrier tube 7; The diameter of this gas distribution grid 6 is 8/10 of solid-state fermenter 5 diameters, the percentage of open area 0.5-07 on the gas distribution grid 6; At horizontal solid state fermentation sealed can 5 one ends recirculation blower 4 and the gas distribution ring 11 relative with gas distribution grid 6 are installed, the diameter of this gas distribution ring 11 is 2/3 of gas distribution grid 6 diameters, and percentage of open area is 0.2-0.5 on the gas distribution ring 11; The other end of solid state fermentation sealed can 5 is the quick-actuating closure door 9 of turnover porous absorption carrier tube 7; In horizontal solid state fermentation sealed shell of tank 5, make jar intravital gas by forming circulation in the porous absorption carrier inner core 7 and between the gas circulation channel 8 by recirculation blower 4; In horizontal solid state fermentation sealed shell of tank 5 outsides, seed liquor in the liquid seeds fermentor tank 1 is inoculated on the porous absorption carrier by Wen's inoculated tube 2; According to humidity conditions in the solid-state fermenter, open the intravital certain humidity of ultrasonic atomizer 3 controlling tank.Wherein percentage of open area is meant that on plate institute's perforate accounts for the ratio of the total area.
Adopt porous absorption carrier process for solid state fermentation of the present utility model to be:
(1) pack in the porous absorption carrier tube 7 in horizontal solid state fermentation sealed shell of tank 5 porous absorption carrier and solubility substratum thereof such as sponge, paper pulp, zeolite and vermiculite, wherein the solid-to-liquid ratio of porous absorption carrier and substratum is 1: 2, be warmed up to 120 ℃ of sterilizations 20-30 minute, ON cycle blower fan 4, after substratum is cooled to 40 ℃, by the seed liquor (with respect to the seed liquor of substratum weight 5%) in the Venturi meter 2 inoculation liquid seeding tanks 1 in inserting the porous absorption carrier, 30 ℃ of temperature in the control porous support, humidity 85%--95% ferments;
(2) in solid ferment process, by certain humidity in the ultrasonic atomizer control tank body, entering jar intravital sterile air air flow is 0.8-1.2vvm (air capacity of per minute feeding tank body is 0.8-1.2 a times of tank body volume);
(3) behind the fermentation termination, open tank body quick-actuating closure door 9, take out porous absorption carrier inner core 7, its porous support and tunning are poured into respectively in three extracting tanks; The extracting solution that at first adds 10 times is in the 1st extractor, and stirring and leaching 1 hour then, is pressed into the 2nd extractor lixiviate with its vat liquor, successively, to the 3rd extractor lixiviate, again vat liquor is made with extra care variant production by traditional separation purification method;
Embodiment 1: porous absorption carrier solid-state fermentation technology clavulanic acid
Pack in the porous absorption carrier tube 7 in horizontal solid state fermentation sealed shell of tank 5 paper pulp porous absorption carrier and solubility substratum thereof (solid-to-liquid ratio is 1: 2.5), be warmed up to 120-125 ℃ of sterilization 20-30 minute, ON cycle blower fan 4, after substratum is cooled to 40 ℃, by the seed liquor (with respect to the seed liquor of substratum weight 5%) in the Venturi meter 2 inoculation liquid seeding tanks in inserting the porous absorption carrier, control temperature 25--28 ℃ in the porous support, humidity 85%--95% ferments; In solid ferment process, by certain humidity in the ultrasonic atomizer control tank body, entering jar intravital sterile air air flow is 1.0vvm (air capacity of per minute feeding tank body is 1.0 times of tank body volume); Behind the fermentation termination, open tank body quick-actuating closure door 9, take out porous absorption carrier inner core 7, its porous support and tunning are poured into respectively in three extracting tanks; The extracting solution that at first adds 10 times is in the 1st extractor, and stirring and leaching 1 hour then, is pressed into the 2nd extractor lixiviate with its vat liquor, successively, to the 3rd extractor lixiviate, again vat liquor is made with extra care variant production by traditional separation purification method;
Bacterial classification: carat dimension streptomycete (Streptomyces clavuligerus);
Porous absorption carrier: wood pulp;
Substratum: soy peptone 0.5%, glycerine 0.8%, KH
2PO
40.1%, FeSO
4.7H
2O0.001%, LiCl 0.8%, pH7.0;
Carrier (solid phase) compares with substratum (liquid phase): 1: 2;
Leavening temperature: 25--28 ℃
Liquid inoculation amount: 10% (with the solid phase ratio);
Fermentation period: 4-5 days
Fermentation titer: 120-150r/g (solid phase);
Fermentation vat liquor purity: 45-53%.
Embodiment 2: porous absorption carrier solid-state fermentation technology genetic engineering bacterium Sumizyme MP
Its operating process is with embodiment 1;
Bacterial classification: Bacillus subtilus (Bacillus subtilis PG1723-3);
Porous absorption carrier: sponge;
Substratum: Zulkovsky starch 0.8%, Tryptones 0.2%, lactose 0.2%, kantlex 0.005%, calcium chloride 0.02%, pH10.5;
Carrier (solid phase) compares with substratum (liquid phase): 1: 1.5;
Leavening temperature: 28--32 ℃
Liquid inoculation amount: 10% (with the solid phase ratio);
Fermentation period: 3--4 days
Fermentation titer: 12160-12350IU/g (solid phase);
Fermentation vat liquor purity: 45--55%.
Embodiment 3: porous absorption carrier solid-state fermentation technology genetic engineering bacterium high-density lipoprotein (HDL) medicine
Its operating process is with embodiment 1;
Bacterial classification: pichia pastoris phaff bacterium (Pichia pastoris YAC);
Porous absorption carrier: sponge or vermiculite;
Substratum: methyl alcohol 0.8%, glycerine 12.5%, oleic acid 0.1%, Histidine 0.1%, ammonium sulfate 1.0%, pH5.5;
Carrier (solid phase) compares with substratum (liquid phase): 1: 1--1.5;
Leavening temperature: 25--27 ℃
Liquid inoculation amount: 10% (with the solid phase ratio);
Fermentation period: 3--4 days
Fermentation titer: 1.12g/g (solid phase);
Fermentation vat liquor purity: 45--50%.
Claims (5)
1, a kind of device that is applied to the described porous absorption carrier of claim 1 process for solid state fermentation, this device comprises recirculation blower (4), solid state fermentation sealed can (5); It is characterized in that in solid state fermentation sealed can (5), being provided with porous absorption carrier tube (7), and be gas circulation channel (8) between solid state fermentation sealed can (5) and the porous absorption carrier tube (7); In porous absorption carrier tube (7), be placed with the porous absorption carrier; Be provided with gas distribution grid (6) at the two ends of porous absorption carrier tube (7), at the preceding gas distribution ring (11) that is provided with of an end gas distribution grid (6) of porous absorption carrier tube (7).
2, porous absorption carrier solid-state fermentation installation as claimed in claim 1 is characterized in that at the preceding recirculation blower (4) of establishing of described gas distribution ring (11).
3, porous absorption carrier solid-state fermentation installation as claimed in claim 1, the diameter that it is characterized in that described gas distribution ring (11) is the 1/2-2/3 of gas distribution grid (6) diameter, it is 0.2-0.5 that gas distribution ring (11) is gone up percentage of open area.
4, porous absorption carrier solid-state fermentation installation as claimed in claim 1, the diameter that it is characterized in that gas distribution grid (6) is the 1/2-8/10 of solid state fermentation sealed can (5) diameter, the percentage of open area on the gas distribution grid (6) is 0.5-07.
5, porous absorption carrier solid-state fermentation installation as claimed in claim 1 is characterized in that described porous absorption carrier is selected from a kind of in sponge, paper pulp, vermiculite, the porous inert material of zeolites.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02285096 CN2587875Y (en) | 2002-11-06 | 2002-11-06 | Porous adsorption carrier solid fermentation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02285096 CN2587875Y (en) | 2002-11-06 | 2002-11-06 | Porous adsorption carrier solid fermentation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2587875Y true CN2587875Y (en) | 2003-11-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02285096 Expired - Lifetime CN2587875Y (en) | 2002-11-06 | 2002-11-06 | Porous adsorption carrier solid fermentation device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102140445A (en) * | 2010-12-31 | 2011-08-03 | 浙江海洋学院 | Carrier cell immobilization method |
| CN102145278A (en) * | 2010-02-09 | 2011-08-10 | 杰智环境科技股份有限公司 | Adsorption system for carbon dioxide |
| CN102643870A (en) * | 2012-05-14 | 2012-08-22 | 中国科学院过程工程研究所 | Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation |
| US10508290B2 (en) | 2012-05-08 | 2019-12-17 | Qvidja Kraft Ab | Means and methods for methane production |
-
2002
- 2002-11-06 CN CN 02285096 patent/CN2587875Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102145278A (en) * | 2010-02-09 | 2011-08-10 | 杰智环境科技股份有限公司 | Adsorption system for carbon dioxide |
| CN102140445A (en) * | 2010-12-31 | 2011-08-03 | 浙江海洋学院 | Carrier cell immobilization method |
| CN102140445B (en) * | 2010-12-31 | 2014-09-03 | 浙江海洋学院 | Carrier cell immobilization method |
| US10508290B2 (en) | 2012-05-08 | 2019-12-17 | Qvidja Kraft Ab | Means and methods for methane production |
| CN102643870A (en) * | 2012-05-14 | 2012-08-22 | 中国科学院过程工程研究所 | Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050622 |
|
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050622 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |