CN212110721U - Water environment DNA sampling device - Google Patents

Water environment DNA sampling device Download PDF

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Publication number
CN212110721U
CN212110721U CN202020594907.6U CN202020594907U CN212110721U CN 212110721 U CN212110721 U CN 212110721U CN 202020594907 U CN202020594907 U CN 202020594907U CN 212110721 U CN212110721 U CN 212110721U
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CN
China
Prior art keywords
water
box body
sample
sealing cover
sampling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN202020594907.6U
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Chinese (zh)
Inventor
陈露
王晶晶
吴炎均
马玉娟
张铭斯
梁沛文
陈方灿
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Guangzhou Qianjiangshui Ecological Technology Co ltd
Original Assignee
Guangzhou Qianjiangshui Ecological Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Guangzhou Qianjiangshui Ecological Technology Co ltd filed Critical Guangzhou Qianjiangshui Ecological Technology Co ltd
Priority to CN202020594907.6U priority Critical patent/CN212110721U/en
Application granted granted Critical
Publication of CN212110721U publication Critical patent/CN212110721U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model relates to a water environment DNA sampling technical field just discloses a water environment DNA sampling device, the power distribution box comprises a box body, the power supply room has been seted up to the bottom of box, the inside fixed mounting of power supply room has the battery, the delivery port has been seted up of box, the sample cell has been seted up on the left of the upper surface of box, the top threaded connection of sample cell has sealed lid one. This sampling device, through the cavity at the right side fixed mounting of water inlet, the impurity of the aquatic of coming is carried to two pairs of delivery hose of the filter of the inside fixed mounting of cavity intercepts, prevent too much impurity when the water pump, cause the jam to the water pump, carry out volume measurement to the water of process through the flowmeter, upload the display screen again, the convenient volume statistics and the record to water, absolute ethyl alcohol through the sample inslot portion can be fine carry out the sample to filter paper and preserve, the quantity in sample groove is 3, can carry out the sampling save many times.

Description

Water environment DNA sampling device
Technical Field
The utility model relates to a water environment DNA sampling technical field specifically is a water environment DNA sampling device.
Background
The application of environmental DNA is firstly to directly extract DNA genome from soil and sediment for researching the diversity of non-culturable microorganisms and identifying species, and in recent years, the application is gradually expanded from microbiology to animal and plant research field, and the environmental DNA is well applied to the research of amphibians, fishes, insect larvae, crustaceans and mammals. In a water body environment, the distribution condition of species can be well judged by utilizing an environmental DNA technology, the community structure of the species is analyzed, particularly under the condition that the biological density of a target species is low, the environmental DNA technology can well capture the DNA of the target species for analysis and research, and the efficiency of the large-scale sequencing analysis technology is far higher than the number of samples obtained by a conventional fishing method. Compared with the traditional investigation method, the environmental DNA technology greatly reduces the statistical work, increases the research accuracy and simultaneously reduces the sample collection cost under the fixed sample number.
At present, in the water body environment DNA sampling device on the market, in the process of sampling, the water pump is blocked due to sundries in the water body, the volume of the extracted water is not well measured, a plurality of filter paper samples cannot be stored at one time, and the error is brought to the following analysis and research.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a water environment DNA sampling device to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a water environment DNA sampling device comprises a box body, wherein a power supply chamber is arranged at the bottom of the box body, a storage battery is fixedly arranged in the power supply chamber, a water outlet is formed in the box body, a sample groove is formed in the left side of the upper surface of the box body, a first sealing cover is in threaded connection with the top end of the sample groove, a sampling groove is formed in the upper surface of the box body, an annular fixing block is fixedly connected to the inner wall of the sampling groove, a fixing ring is fixedly connected to the inner part of the annular fixing block, filter paper is placed on the upper surface of the fixing ring, a second sealing cover is in threaded connection with the top end of the sampling groove, a fixing rod is fixedly arranged at the bottom of the second sealing cover, a rubber ring is fixedly arranged at the bottom end of the fixing rod, a filter tank is formed in the upper surface of, the last fixed surface of box installs the display screen, the bottom fixed mounting of display screen has fluidflowmeter, the last surface right side fixed mounting of box has the apron, the inside right side fixed mounting of box has the hose connector, the inside right side fixed mounting of box has the water pump, the water inlet has been seted up on the right side of box, the right-hand member fixed mounting of water inlet has the cavity, the inside fixed mounting of cavity has filter two, the right side of cavity is pegged graft and is had conveying hose.
Preferably, the inner walls of the upper ports of the sample tank, the sampling tank and the filtering tank are fixedly connected with annular blocks, inner spiral threads are formed in the annular blocks, and outer spiral threads are formed in the outer walls of the first sealing cover, the second sealing cover and the third sealing cover.
Preferably, the water inlet, the water pump, the liquid flowmeter, the filter tank, the sampling tank and the water outlet are connected in sequence through hoses.
Preferably, the left side of the bottom of the filter tank pair is provided with a groove, a filter plate is fixedly arranged in the groove, and the left side of the groove is provided with a water delivery port.
Preferably, the number of the sample groove pairs is 3, the top end of each sample groove is in threaded connection with a first sealing cover, and a proper amount of absolute ethyl alcohol is stored in each sample groove.
Compared with the prior art, the beneficial effects of the utility model are that: this water environment DNA sampling device, cavity through the right side fixed mounting at the water inlet, the impurity of the aquatic of coming is carried to two pairs of delivery hose to the filter of the inside fixed mounting of cavity intercepts, prevent too much impurity when through the water pump, cause the jam to the water pump, the filter tank left side wall fixed mounting filter carries out the impurity interception of second time to it, prevent too much impurity from getting into the sampling groove, influence its sampling, carry out volume measurement to the water of process through the flowmeter, upload to the display screen again, the convenience is to the volume statistics and the record of water, absolute ethyl alcohol through the sample inslot portion can be fine carry out the sample to filter paper and preserve, the quantity in sample groove is 3, can carry out the sampling save many times.
Drawings
FIG. 1 is a schematic sectional view of the present invention;
FIG. 2 is a schematic view of the internal structure of the sampling tank of the present invention;
fig. 3 is a schematic view of the top view structure of the present invention.
In the figure: the device comprises a box body 1, a storage battery 2, a power supply chamber 3, a water outlet 4, a sample tank 5, a first sealing cover 6, a sampling tank 7, a second sealing cover 8, a filter plate 9, a filter tank 10, a third sealing cover 11, a liquid flowmeter 12, a display screen 13, a cover plate 14, a hose connector 15, a water pump 16, a water inlet 17, a conveying hose 18, a second filter plate 19, a cavity 20, a fixing rod 21, a rubber ring 22, an annular fixing block 23, filter paper 24 and a fixing ring 25.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: a water environment DNA sampling device comprises a box body 1, a power supply chamber 3 is arranged at the bottom of the box body 1, a storage battery 2 is fixedly arranged in the power supply chamber 3, a water outlet 4 is arranged in the box body 1, a sample groove 5 is arranged on the left side of the upper surface of the box body 1, a first sealing cover 6 is in threaded connection with the top end of the sample groove 5, the number of the sample groove 5 pairs is 3, the top end of each sample groove is in threaded connection with a first sealing cover, a proper amount of absolute ethyl alcohol is stored in the sample groove 5, the absolute ethyl alcohol in the sample groove 5 can well store samples on filter paper 24, the number of the sample grooves 5 is 3, multiple sampling and storage can be carried out, a sampling groove 7 is arranged on the upper surface of the box body 1, an annular fixing block 23 is fixedly connected with the inner wall of the sampling groove 7, a fixing ring 25 is fixedly connected with the, the top end of the sampling groove 7 is in threaded connection with a second sealing cover 8, the bottom of the second sealing cover 8 is fixedly provided with a fixed rod 21, the bottom end of the fixed rod 21 is fixedly provided with a rubber ring 22, the upper surface of the box body 1 is provided with a filter groove 10, the upper surface of the filter groove 10 is in threaded connection with a third sealing cover 11, the inner walls of the upper ports of the sample groove 5, the sampling groove 7 and the filter groove 10 are fixedly connected with an annular block, the inner part of the annular block is provided with inner spiral threads, the outer walls of the first sealing cover 6, the second sealing cover 8 and the third sealing cover 11 are provided with outer spiral threads, the left side of the inner part of the filter groove 10 is fixedly provided with a filter plate 9, the left side of the bottom of the filter groove 10 pair is provided with a groove, the filter plate 9 is fixedly arranged in the groove, the left wall of the filter, the sampling of the water purifier is influenced, a display screen 13 is fixedly arranged on the upper surface of a box body 1, a liquid flowmeter 12 is fixedly arranged at the bottom of the display screen 13, the flowmeter 12 measures the volume of water passing through and then uploads the water to the display screen 13, the volume statistics and recording of water are facilitated, a cover plate 14 is fixedly arranged on the right side of the upper surface of the box body 1, a hose connector 15 is fixedly arranged on the right side of the interior of the box body 1, a water pump 16 is fixedly arranged on the right side of the interior of the box body 1, a water inlet 17 is formed in the right side of the box body 1, a cavity 20 is fixedly arranged at the right end of the water inlet 17, a second filter plate 19 fixedly arranged in the cavity 20 intercepts impurities in the water conveyed by a conveying hose 18, the water pump is prevented from being blocked by excessive impurities when the water pump 16 passes through, and the second, the right side of the cavity 20 is inserted with a conveying hose 18, and the water inlet 17, the water pump 16, the liquid flowmeter 12, the filter tank 10, the sampling tank 7 and the water outlet 4 are connected in sequence through hoses.
In the actual operation process, when the device uses, the water pump 16 is started to extract and convey water, the water flow is recorded in volume when passing through the liquid flow meter 12 and is uploaded to the display screen 13, then the water enters the filter tank 10, the water is filtered by the filter plate 9 on the left side inside the filter tank 10, the water enters the sampling tank 7 after being filtered, the water finally flows to the water outlet 4 through the filter paper 24 inside the sampling tank 7 and is discharged, and after sampling is completed, the filter paper 24 is taken out and is stored in the absolute ethyl alcohol in the sample tank 5.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. The utility model provides a water environment DNA sampling device, includes box (1), its characterized in that: the bottom of the box body (1) is provided with a power supply chamber (3), a storage battery (2) is fixedly mounted inside the power supply chamber (3), a water outlet (4) is formed in the box body (1), a sample groove (5) is formed in the left side of the upper surface of the box body (1), a first sealing cover (6) is connected to the top end of the sample groove (5) in a threaded manner, a sampling groove (7) is formed in the upper surface of the box body (1), an annular fixing block (23) is fixedly connected to the inner wall of the sampling groove (7), a fixing ring (25) is fixedly connected to the inside of the annular fixing block (23), filter paper (24) is placed on the upper surface of the fixing ring (25), a second sealing cover (8) is connected to the top end of the sampling groove (7) in a threaded manner, a fixing rod (21) is fixedly mounted at the bottom of the second sealing, the improved water purifier is characterized in that a filter tank (10) is arranged on the upper surface of the box body (1), a third sealing cover (11) is in threaded connection with the upper surface of the filter tank (10), a filter plate (9) is fixedly arranged on the left inside of the filter tank (10), a display screen (13) is fixedly arranged on the upper surface of the box body (1), a liquid flow meter (12) is fixedly arranged at the bottom of the display screen (13), a cover plate (14) is fixedly arranged on the right side of the upper surface of the box body (1), a hose connecting port (15) is fixedly arranged on the right inside of the box body (1), a water pump (16) is fixedly arranged on the right inside of the box body (1), a water inlet (17) is arranged on the right side of the box body (1), a cavity (20) is fixedly arranged at the right end of the water inlet, the right side of the cavity (20) is inserted with a conveying hose (18).
2. The water body environment DNA sampling device of claim 1, wherein: the inner wall of the upper port of the sample tank (5), the inner wall of the upper port of the sampling tank (7) and the inner wall of the upper port of the filtering tank (10) are fixedly connected with annular blocks, inner spiral threads are arranged inside the annular blocks, and outer spiral threads are arranged on the outer walls of the sealing cover I (6), the sealing cover II (8) and the sealing cover III (11).
3. The water body environment DNA sampling device of claim 1, wherein: the water inlet (17), the water pump (16), the liquid flowmeter (12), the filter tank (10), the sampling tank (7) and the water outlet (4) are connected in sequence through hoses.
4. The water body environment DNA sampling device of claim 1, wherein: the left sides of the bottoms of the filter tank pairs (10) are provided with grooves, filter plates (9) are fixedly arranged in the grooves, and water conveying ports are formed in the left sides of the grooves.
5. The water body environment DNA sampling device of claim 1, wherein: the number of the sample groove (5) pairs is 3, the top end of each sample groove is in threaded connection with a first sealing cover, and a proper amount of absolute ethyl alcohol is stored in each sample groove (5).
CN202020594907.6U 2020-04-20 2020-04-20 Water environment DNA sampling device Expired - Fee Related CN212110721U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202020594907.6U CN212110721U (en) 2020-04-20 2020-04-20 Water environment DNA sampling device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202020594907.6U CN212110721U (en) 2020-04-20 2020-04-20 Water environment DNA sampling device

Publications (1)

Publication Number Publication Date
CN212110721U true CN212110721U (en) 2020-12-08

Family

ID=73640752

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202020594907.6U Expired - Fee Related CN212110721U (en) 2020-04-20 2020-04-20 Water environment DNA sampling device

Country Status (1)

Country Link
CN (1) CN212110721U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117327561A (en) * 2023-10-16 2024-01-02 中国环境科学研究院 Water environment DNA acquisition facility

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117327561A (en) * 2023-10-16 2024-01-02 中国环境科学研究院 Water environment DNA acquisition facility

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CF01 Termination of patent right due to non-payment of annual fee
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Granted publication date: 20201208

Termination date: 20210420