CN117327561A - Water environment DNA acquisition facility - Google Patents

Abstract

The invention discloses a water environment DNA collection equipment, which includes a lower box body. A clean water chamber, a motor room, a water pump room and a power supply room are arranged in the lower box body. A motor is arranged in the motor room, and a water pump is arranged in the water pump room. The lower box body is fixed A first filtering mechanism is connected, the water pump is connected to the first filtering mechanism, a support column is fixedly connected to the outside of the lower box, and a turntable is fixedly connected to the support column; there are several collection components, and the collection components include a filter press, a cylinder and a bottom shell. There is a horizontal plate fixedly connected to the cylinder, which divides the cylinder into a filter chamber and a filter chamber. A second filter mechanism is provided in the bottom shell. A water inlet hole is provided on the bottom shell. The water pump and the water inlet hole pass through the first soft filter. The pipes are connected, the first hose is detachably connected to the water inlet, the filter press is detachably connected to the cylinder, the filter press is connected to the filter chamber, and a filter membrane is provided in the filter press. The invention can reduce manual operations when collecting environmental DNA, and can collect multiple samples at the same time, making it more convenient to use.

Description

A kind of water environment DNA collection equipment

Technical field

The invention relates to the field of environmental DNA collection, and in particular to a water environment DNA collection equipment.

Background technique

Environmental DNA (eDNA) refers to the sum of DNA fragments that can be directly extracted from environmental samples (such as water, soil, air, ice cores, etc.). It is a mixture of DNA from different species such as microorganisms, animals, plants, etc., including both organisms. Intracellular DNA released into epidermal cells in the environment through skin, urine, feces, mucus, etc. also includes extracellular DNA released into the environment after cell death and cleavage. DNA is captured from environmental samples and preserved, extracted, amplified, sequenced and classified to determine the distribution of organisms in the sampled environment.

At present, the main method of collecting eDNA in water bodies is to collect a certain volume of water samples to be tested, and then bring the water samples back to the laboratory. The experimenters manually filter the eDNA in the water samples to collect the eDNA. The filtration process is inconvenient to operate. If multiple copies of DNA are needed, Samples require repeated operations many times, and the work efficiency is relatively low. Therefore, a water environment DNA collection device is proposed to solve the above problems.

Contents of the invention

The purpose of the present invention is to provide a water environment DNA collection device to solve the problems existing in the above-mentioned existing technologies and facilitate the collection of environmental DNA.

In order to achieve the above objects, the present invention provides the following solutions: The present invention provides a water environment DNA collection equipment, including:

The lower box body is provided with a clean water room, a motor room, a water pump room and a power supply room. The motor room is equipped with a motor, and the water pump room is equipped with a water pump. The lower box body is fixedly connected with a first Filtration mechanism, the water pump is connected with the first filter mechanism, a support column is fixedly connected to the lower box outside the body, and a turntable is fixedly connected to the support column;

Collection components, the collection components are in several groups. The collection components include a filter press, a cylinder and a bottom shell. A horizontal plate is fixedly connected to the cylinder, and the horizontal plate divides the cylinder into a filter pressure chamber and a bottom shell. A filter chamber, a second filter mechanism is provided in the bottom shell, the bottom shell is detachably connected to the cylinder, the bottom shell is detachably connected to the turntable, and a water inlet hole is provided on the bottom shell , the water pump and the water inlet are connected through a first hose, the first hose is detachably connected to the water inlet, the filter press is detachably connected to the cylinder, the filter press Communicated with the filter cavity, a filter membrane is provided in the filter press, and an electric telescopic rod is fixedly connected to the support column, and the electric telescopic rod is drivingly connected to the filter press.

Preferably, the filter press includes an inner cylinder, the inner cylinder is detachably connected to the cylinder body, the inner cylinder is located in the filter press chamber, and a piston is slidably connected to the inner cylinder. A push-pull rod is fixedly connected, a first groove is provided on the inner cylinder, a filter membrane is placed in the first groove, a pressure ring is detachably connected to the first groove, and the pressure ring is connected to the The filter membrane is in contact with the piston. A first through hole is provided on the piston. A first hard pipe is fixedly connected to the first through hole. A first one-way valve is installed on the first hard pipe. The first through hole is fixedly connected to the first hard pipe. A second flexible tube is connected to the hard tube, and the second flexible tube is connected to the filter chamber.

Preferably, a plurality of connecting rods are fixedly connected to the inner cylinder, and a positioning ring is fixedly connected to the connecting rods. The push-pull rod is located in the positioning ring, and the push-pull rod is movable within the positioning ring. A fixed plate is fixedly connected to the push-pull rod. A first spring is provided between the fixed plate and the positioning ring. The first spring is passed through the push-pull rod. The filter press chamber and the filter The cavities are all provided with air holes, and the air holes are fixedly connected with tracheas.

Preferably, a top block is fixedly connected to the top of the push-pull rod, the top block has a first slope, a supporting plate is fixedly connected to the support column, and a push block is fixedly connected to the output end of the electric telescopic rod. The block has a second slope, the push block is slidably arranged on the supporting plate, and an escape groove is provided on the push block.

Preferably, the second filtering mechanism includes a filter screen and a filter cotton, a second groove and a third groove are provided in the bottom shell, and the filter screen and the filter cotton are detachably connected to the third groove. In the second groove, the filter cotton is located above the filter mesh, the side wall of the third groove is provided with threads, the outer edge of the cylinder is provided with threads, and the cylinder and the third groove pass through The threads are detachably connected, and a second through hole is provided on the side wall of the filter chamber. A second hard pipe is inserted through the second through hole, and the water inlet end of the second hard pipe is located above the filter cotton. The second flexible tube is fixedly connected to the second hard tube.

Preferably, the first filtering mechanism includes a filter box, the filter box is fixedly connected to the lower box body, an interception net is fixedly connected to the filter box, and a water outlet hole is provided on the filter box. The water outlet is connected to the water pump through a third hose, and the third hose is detachably connected to the water outlet. A rotating shaft is fixedly connected to the output end of the motor, and the rotating shaft extends out of the lower box body. The rotating shaft passes through the filter box and the intercepting net. A brush is fixedly connected to the rotating shaft, and the brush is in contact with the intercepting net.

Preferably, the turntable is fixedly connected with an installation groove, the bottom shell is detachably connected in the installation groove, a first cavity is opened in the side wall of the installation groove, and the first cavity side wall is provided with a first cavity. A third through hole is provided, and a clamping column is inserted through the third through hole and the first cavity. A baffle is fixedly connected to the clamping column, and the baffle is in the first cavity. , a second spring is set on the clamping column, the second spring is located in the first cavity, one end of the second spring is in contact with the baffle, and the other end of the second spring is in contact with the baffle. The side walls of the first cavity are in contact with each other, and a clamping slot is provided on the bottom shell. The clamping column is detachably connected to the clamping slot, and the end of the clamping column away from the clamping slot is fixedly connected to a hand-operated block.

Preferably, the lower box is fixedly connected to a number of guide posts outside the body, a movable plate is slidably connected to the guide posts, studs are rotatably connected to the lower box body, and threaded holes are provided on the movable plate. The stud is passed through the threaded hole, and the stud is movable in the threaded hole. A turbine is fixedly connected to the stud, and a worm is rotatably connected to the lower box body. The turbine is connected to the threaded hole. The worm is engaged, a first joint is fixedly connected to the movable plate, the first joint is connected to the first hose, a through hole is provided in the installation groove and the turntable, and the water inlet hole is A second connector is fixedly connected, the second connector is inserted into the via hole, and the first connector is detachably connected to the via hole.

Preferably, the lower box is fixedly connected to two vertical plates outside the body, the worm is rotatably connected to the vertical plates, a number of uprights are fixedly connected to the lower box, and a second cavity is provided in the uprights. A fourth through hole is opened on the top wall of the second cavity. A sphere and a third spring are placed in the second cavity. The sphere extends out of the fourth through hole. The sphere is in contact with the third spring. Three springs are in contact with each other, a positioning groove is provided on the turntable, and the ball is clamped in the positioning groove.

Preferably, a plurality of partitions are fixedly connected to the lower box, and the partitions divide the lower box into the clean water room, the motor room, the water pump room and the power supply room. Clean water is installed in the room, and a power supply is provided in the power supply room. The motor and the electric telescopic rod are electrically connected to the power supply. A water adding hole is provided on the side wall of the clean water chamber, and the water adding hole is fixedly connected. There is a water adding pipe, a second one-way valve is installed on the water adding pipe, a drainage pipe is provided in the clean water chamber, the drainage pipe extends out of the lower box, and the drainage pipe and the third hose can After disassembly and connection, an upper plate is fixedly connected to the top of the support column, and a handle is fixedly connected to the upper plate.

The invention discloses the following technical effects: In this device, the first filtering mechanism filters water for the first time, the water pump sends the first filtered water to the bottom shell, and the filter membrane in the filter press is used to filter DNA in the water. , the cylinder is used to connect the filter press and the bottom shell. The second filtering mechanism in the bottom shell filters the water for the second time. The second filtered water is used to extract DNA from the water. The electric telescopic rod drives the filter press to move. For inhaling and pressure filtering the water after the second filtering, the collection components are divided into multiple groups. After using one group, the turntable can be rotated to continue using the other group. The used collection components can also be removed from the turntable. The invention can reduce manual operations when collecting environmental DNA, and can collect multiple samples at the same time, making it more convenient to use.

Description of drawings

In order to explain the embodiments of the present invention or the technical solutions in the prior art more clearly, the drawings needed to be used in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some of the drawings of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained based on these drawings without exerting creative efforts.

Figure 1 is a schematic structural diagram of the water environment DNA collection equipment of the present invention;

Figure 2 is a partial enlarged view of a in Figure 1;

Figure 3 is a partial enlarged view of b in Figure 1;

Figure 4 is a partial enlarged view of c in Figure 1;

Figure 5 is a partial enlarged view of d in Figure 1;

Among them, 1. Lower box; 2. Clean water chamber; 3. Motor room; 4. Motor; 5. Water pump; 6. Support column; 7. Turntable; 8. Cylinder; 9. Bottom shell; 10. Horizontal plate; 11. Press filter chamber; 12. Filter chamber; 13. Water inlet; 14. First hose; 15. Filter membrane; 16. Electric telescopic rod; 17. Inner cylinder; 18. Piston; 19. Push-pull rod; 20 , pressure ring; 21. first hard pipe; 22. connecting rod; 23. positioning ring; 24. fixed plate; 25. first spring; 26. air pipe; 27. top block; 28. support plate; 29. push block ; 30. Filter; 31. Filter cotton; 32. Second hard pipe; 33. Second hose; 34. Filter box; 35. Interceptor net; 36. Third hose; 37. Rotating shaft; 38. Brush ; 39. Installation slot; 40. First cavity; 41. Clamping column; 42. Baffle; 43. Second spring; 44. Hand-operated block; 45. Guide pillar; 46. Movable plate; 47. Stud; 48. Turbine; 49. Worm; 50. First joint; 51. Via hole; 52. Vertical plate; 53. Column; 54. Sphere; 55. Third spring; 56. Partition plate; 57. Power supply; 58. Addition Water pipe; 59, drainage pipe; 60, upper plate; 61, first one-way valve.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

In order to make the above objects, features and advantages of the present invention more obvious and understandable, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

Referring to Figures 1-5, the present invention provides a water environment DNA collection device, including:

The lower box body 1 is provided with a clean water room 2, a motor room 3, a water pump room and a power supply room. The motor room 3 is provided with a motor 4, and the water pump room is provided with a water pump 5. The lower box body 1 is fixedly connected with The first filtering mechanism, the water pump 5 is connected with the first filtering mechanism, the lower box body 1 is fixedly connected to the support column 6, and the support column 6 is fixedly connected to the turntable 7;

The collection assembly is divided into several groups. The collection assembly includes a filter press, a cylinder 8 and a bottom shell 9. A horizontal plate 10 is fixedly connected to the cylinder 8. The horizontal plate 10 divides the cylinder 8 into a filter pressure chamber 11 and a filter chamber. 12. A second filtering mechanism is provided in the bottom shell 9. The bottom shell 9 is detachably connected to the cylinder 8. The bottom shell 9 is detachably connected to the turntable 7. A water inlet hole 13 is provided on the bottom shell 9. The water pump 5 is connected to the water inlet. The hole 13 is connected through the first hose 14, the first hose 14 is detachably connected to the water inlet 13, the filter press is detachably connected to the cylinder 8, the filter press is connected to the filter chamber 12, and a filter membrane 15 is provided in the filter press. , the support column 6 is fixedly connected with an electric telescopic rod 16, and the electric telescopic rod 16 is drivingly connected to the filter press.

The first filtering mechanism filters the water for the first time. The water pump 5 pumps the first filtered water into the bottom shell 9. The filter membrane 15 in the filter press is used to filter DNA in the water. The cylinder 8 is used to connect the filter press. and the bottom shell 9. The second filtering mechanism in the bottom shell 9 filters the water for the second time. The second filtered water is used to extract DNA in the water. The electric telescopic rod 16 drives the filter press to move and is used for suction and press filtering. The water after filtering for the second time has multiple collection components. After using one group, the turntable 7 can be rotated to continue using another group. The used collection components can also be removed from the turntable 7 .

To further optimize the solution, the filter press includes an inner cylinder 17. The inner cylinder 17 is detachably connected to the cylinder 8. The inner cylinder 17 is located in the filter press chamber 11. A piston 18 is slidably connected to the inner cylinder 17, and a push button is fixedly connected to the piston 18. The pull rod 19 and the inner cylinder 17 are provided with a first groove. A filter membrane 15 is placed in the first groove. A pressure ring 20 is detachably connected to the first groove. The pressure ring 20 is in contact with the filter membrane 15. The piston 18 is on A first through hole is provided, a first hard pipe 21 is fixedly connected to the first through hole, a first one-way valve 61 is installed on the first hard pipe 21, and a second hose 33 is connected to the first hard pipe 21. The second hose 33 is connected with the filter chamber 12 .

The inner cylinder 17 and the cylinder 8 are connected through threads, making connection and disassembly more convenient. The push-pull rod 19 is used to drive the piston 18 to move, and the pressure ring 20 is used to press the filter membrane 15. The pressure ring 20 is connected to the first groove through threads. The first hard tube 21 is detachably connected to the second flexible tube 33. The electric telescopic rod 16 drives the push-pull rod 19 to move. When the push-pull rod 19 rises, the piston 18 rises, and the gap between the piston 18 and the filter membrane 15 becomes larger. The second filtered water is sucked in. When the push-pull rod 19 moves down, the water inside will be squeezed. At the same time, the first one-way valve 61 can prevent the water from flowing out. After the use of the filter membrane 15 is completed, the pressure ring 20 can be Remove, and then take off the filter membrane 15.

To further optimize the solution, there are a number of connecting rods 22 fixedly connected to the inner cylinder 17. The connecting rods 22 are fixedly connected to a positioning ring 23. The push-pull rod 19 is located in the positioning ring 23. The push-pull rod 19 is movable in the positioning ring 23. The push-pull rod 19 is A fixed plate 24 is fixedly connected. A first spring 25 is provided between the fixed plate 24 and the positioning ring 23. The first spring 25 is passed through the push-pull rod 19. Both the filter pressure chamber 11 and the filter chamber 12 are provided with air holes. A trachea 26 is fixedly connected to the top.

The positioning ring 23 is used to stabilize the push-pull rod 19. When the electric telescopic rod 16 drives the push-pull rod 19 to move upward, the fixed plate 24 also moves upward, which compresses the first spring 25. When the electric telescopic rod 16 leaves the push-pull rod 19, at the first Under the action of the spring 25, the fixed plate 24 drives the push-pull rod 19 to move downward, thereby squeezing the inhaled water, which can speed up the filtration of water by the filter membrane 15. The air holes facilitate the discharge of the air in the filter chamber 11 and the filter chamber 12, which is convenient. Water entry and exit.

To further optimize the solution, a top block 27 is fixedly connected to the top of the push-pull rod 19. The top block 27 has a first slope, a supporting plate 28 is fixedly connected to the support column 6, and a push block 29 is fixedly connected to the output end of the electric telescopic rod 16. The push block 29 It has a second inclined surface, the push block 29 is slidably arranged on the supporting plate 28, and an escape groove is provided on the push block 29.

The electric telescopic rod 16 drives the push block 29 to move. When the push block 29 contacts the top block 27, the second slope of the push block 29 will contact the first slope of the push block 27. As the push block 29 moves, the push block 29 will move. The block 27 pushes up, thus driving the push-pull rod 19 to move upward. The purpose of the avoidance groove is to avoid the push-pull rod 19 and prevent the push block 29 from being blocked in its movement.

In a further optimized solution, the second filtering mechanism includes a filter screen 30 and a filter cotton 31. A second groove and a third groove are provided in the bottom case 9. Both the filter screen 30 and the filter cotton 31 are detachably connected in the second groove. , the filter cotton 31 is located above the filter screen 30, there are threads on the side wall of the third groove, and there are threads on the outer edge of the cylinder 8. The cylinder 8 and the third groove are detachably connected through threads, and there are threads on the side wall of the filter chamber 12. A second through hole is provided, and a second hard pipe 32 is inserted through the second through hole. The water inlet end of the second hard pipe 32 is located above the filter cotton 31 , and the second flexible pipe 33 is fixedly connected to the second hard pipe 32 .

The filter screen 30 and the filter cotton 31 filter the water sample for the second time to prevent impurities in the water from affecting the collection of DNA. The filter screen 30 and the filter cotton 31 are clamped in the second groove to facilitate the filter screen 30 and the filter cotton 31. For replacement, the barrel 8 and the bottom shell 9 are connected through threads, making installation and disassembly more convenient. The second hard tube 32 and the second hose 33 are connected to facilitate the connection and disassembly of the second hose 33.

To further optimize the solution, the first filtering mechanism includes a filter box 34. The filter box 34 is fixedly connected to the lower box body 1. The filter box 34 is fixedly connected to an interception net 35. The filter box 34 is provided with a water outlet through which the water pump 5 passes. The third hose 36 is connected, and the third hose 36 is detachably connected to the water outlet. The output end of the motor 4 is fixedly connected with a rotating shaft 37. The rotating shaft 37 extends out of the lower box body 1, and the rotating shaft 37 passes through the filter box 34 and the interception net 35. A brush 38 is fixedly connected to the rotating shaft 37 , and the brush 38 is in contact with the intercepting net 35 .

The interception net 35 is used to intercept larger impurities. The third hose 36 is used to connect the filter box 34 and the water pump 5. The motor 4 drives the rotating shaft 37 to rotate. The rotating shaft 37 drives the brush 38 to rotate. The brush 38 can wash the intercepting network 35. of debris to prevent blocking the interception net 35.

To further optimize the solution, a mounting slot 39 is fixedly connected to the turntable 7, and the bottom shell 9 is detachably connected in the mounting slot 39. A first cavity 40 is provided in the side wall of the mounting slot 39, and a first cavity 40 is provided on the side wall of the first cavity 40. The third through hole, the third through hole and the first cavity 40 are provided with clamping pillars 41. The clamping pillars 41 are fixedly connected with a baffle 42. The baffle 42 is in the first cavity 40, and the clamping pillars 41 are covered. A second spring 43 is provided. The second spring 43 is located in the first cavity 40. One end of the second spring 43 is in contact with the baffle 42, and the other end of the second spring 43 is in contact with the side wall of the first cavity 40. The bottom shell 9 is provided with a card slot, the card post 41 is detachably connected to the card slot, and the end of the card post 41 away from the card slot is fixedly connected to the hand block 44.

The bottom shell 9 is detachably connected in the installation groove 39. When installing the bottom shell 9, pull the hand-operated block 44 outward, and the hand-operated block 44 drives the clamping column 41 to move. When the clamping column 41 completely enters the third through hole, it can be Put the bottom shell 9 into the installation groove 39. When the clamping column 41 moves, it will drive the baffle 42 to move. The movement of the baffle 42 will compress the second spring 43. After releasing the handle block 44, the second spring 43 will move. Under the action, the end of the clamping column 41 will enter the clamping groove, thereby clamping the bottom case 9 into the column.

To further optimize the solution, a number of guide posts 45 are fixedly connected to the outside of the lower box body 1, and a movable plate 46 is slidably connected to the guide posts 45. A stud 47 is rotatably connected to the lower box body 1, and a threaded hole is provided on the movable plate 46. The column 47 is penetrated in the threaded hole, the stud 47 is movable in the threaded hole, the stud 47 is fixedly connected with a turbine 48, the lower box body 1 is rotatably connected with a worm 49, the turbine 48 meshes with the worm 49, and the movable plate 46 A first joint 50 is fixedly connected to the top, and the first joint 50 is connected with the first hose 14. A through hole 51 is provided in the installation groove 39 and the turntable 7. A second joint is fixedly connected to the water inlet hole 13, and the second joint is inserted into the water inlet hole 13. Connected in the via hole 51 , the first connector 50 is detachably connected to the via hole 51 , and the top of the guide post 45 is fixedly connected with a blocking block.

When the bottom case 9 is put into the installation slot 39, the second joint will be inserted into the through hole 51. The movable plate 46 drives the first joint 50 to move. The first joint 50 is used to connect with the through hole 51. When connecting, the worm 49 is rotated , the worm 49 drives the turbine 48 to rotate, the turbine 48 drives the stud 47 to rotate, the stud 47 drives the movable plate 46 to move up and down, the stud 47 is rotationally connected with the lower box body 1, when the stud 47 rotates, the stud 47 is not in the lower box To move the position on the body 1, when rotating the turntable 7, it is necessary to disassemble the second joint and the through hole 51, and then rotate the worm 49 in the opposite direction.

To further optimize the solution, two vertical plates 52 are fixedly connected to the outside of the lower box 1, and the worm 49 is rotatably connected to the vertical plates 52. There are a number of uprights 53 fixedly connected to the lower box 1, and a second cavity is provided in the uprights 53. A fourth through hole is provided on the top wall of the second cavity. A ball 54 and a third spring 55 are placed in the second cavity. The ball 54 extends out of the fourth through hole. The ball 54 is in contact with the third spring 55. On the turntable 7 A positioning groove is provided, and the ball 54 is clamped in the positioning groove.

The vertical plate 52 is used to support the worm 49, and the ball 54 is used to contact the turntable 7. When the turntable 7 is rotated, the ball 54 will move on the surface of the turntable 7. When the ball 54 enters the positioning groove, the turntable 7 will be stuck, preventing The turntable 7 rotates, and the ball 54 can leave the positioning groove by rotating the turntable 7 forcefully.

To further optimize the solution, a number of partitions 56 are fixedly connected to the lower box 1. The partitions 56 divide the lower box 1 into a clean water room 2, a motor room 3, a water pump room 5 and a power supply room. The clean water room 2 contains clean water. , a power supply 57 is provided in the room. The motor 4 and the electric telescopic rod 16 are electrically connected to the power supply 57. A water-adding hole is provided on the side wall of the clean water chamber. A water-adding pipe 58 is fixedly connected in the water-adding hole. A third water-adding pipe 58 is installed on the water-adding pipe 58. Two one-way valves, a drainage pipe is provided in the clean water chamber, the drainage pipe extends out of the lower box body 1, the drainage pipe is detachably connected to the third hose 36, the top of the support column 6 is fixedly connected to an upper plate 60, and the upper plate 60 is fixedly connected to handle.

The partition 56 is used to separate the clean water room 2, the motor room 3, the water pump room 5 and the power supply room 57. The clean water is used to flush the third hose 36, the water pump 5 and the first hose 14. When a set of filter press sampling is completed , take the device out of the water, remove the bottom shell 9 from the turntable 7, and then connect the third hose 36 to the drainage pipe of the clean water room, then turn on the water pump 5 and flush the pipeline to prevent it from affecting the next collection. The water adding pipe 58 facilitates adding water to the clean water chamber.

To use this device, put the device into the water in the target area, and then put the device underwater. The water enters the filter box 34. The interceptor net 35 filters the water for the first time. After starting the water pump 5, the water pump 5 will filter the water. It is sent into the bottom case 9 through the first hose 14. There is a filter screen 30 and a filter cotton 31 in the bottom case 9. The filter screen 30 and the filter cotton 31 filter the water sample for the second time. The electric telescopic rod 16 is started and the electric telescopic rod 16 is started. The rod 16 drives the push block 29 to move. When the push block 29 contacts the top block 27, the second slope of the push block 29 will contact the first slope of the push block 27. As the push block 29 moves, the push block 27 will move. When the push-pull rod 19 rises, the piston 18 rises, and the gap between the piston 18 and the filter membrane 15 becomes larger. It will enter between the piston 18 and the filter membrane 15 through the second hard tube 32, the second flexible tube 33, and the first hard tube 21. After the water enters, the electric telescopic rod 16 is retracted, and the push block 29 and the top block 27 are separated. Under the action of the first spring 25, the fixed plate 24 drives the push-pull rod 19 to move downward, thereby squeezing the inhaled water, which can speed up the filtration of water by the filter membrane 15. After a set of filter press sampling is completed, take out the device from the water, remove the bottom shell 9 from the turntable 7, rotate the worm 49 in the reverse direction, the worm 49 drives the turbine 48 to rotate, the turbine 48 drives the stud 47 to rotate, and the movable plate 46 moves down, disassembles the second joint and through hole 51, then rotates the turntable 7, and replaces another set of filter press, cylinder 8 and bottom shell 9.

In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientations or positional relationships indicated by "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention, rather than indicating or It is implied that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation and is therefore not to be construed as a limitation of the invention.

The above-described embodiments only describe the preferred modes of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, those of ordinary skill in the art can make various modifications to the technical solutions of the present invention. All deformations and improvements shall fall within the protection scope determined by the claims of the present invention.

Claims (10)

1. A water environment DNA collection equipment, characterized by including: Lower box body (1), the lower box body (1) is provided with a clean water room (2), a motor room (3), a water pump room and a power supply room, and the motor room (3) is provided with a motor (4) , a water pump (5) is provided in the water pump chamber, a first filtering mechanism is fixedly connected to the lower box (1), the water pump (5) is connected to the first filtering mechanism, and the lower box (1) 1) A support column (6) is fixedly connected to the outside, and a turntable (7) is fixedly connected to the support column (6); The collection assembly is composed of several groups. The collection assembly includes a filter press, a cylinder (8) and a bottom shell (9). A horizontal plate (10) is fixedly connected to the cylinder (8). The horizontal plate (10) divides the cylinder (8) into a filter chamber (11) and a filter chamber (12). A second filter mechanism is provided in the bottom shell (9). The bottom shell (9) is detachably connected to the cylinder (8), the bottom shell (9) is detachably connected to the turntable (7), the bottom shell (9) is provided with a water inlet hole (13), and the water pump (5) is connected to the water inlet (13) through a first hose (14). The first hose (14) is detachably connected to the water inlet (13). The filter press is connected to the water inlet (13). The cylinder (8) is detachably connected, the filter press is connected with the filter chamber (12), a filter membrane (15) is provided in the filter press, and the support column (6) is fixedly connected with an electric telescopic Rod (16), the electric telescopic rod (16) is drivingly connected to the filter press. 2. A water environment DNA collection device according to claim 1, characterized in that: the filter press includes an inner cylinder (17), and the inner cylinder (17) is detachably connected to the cylinder (8) Inside, the inner cylinder (17) is located in the filter pressure chamber (11). A piston (18) is slidably connected in the inner cylinder (17), and a push-pull rod (19) is fixedly connected to the piston (18). ), the inner cylinder (17) is provided with a first groove, a filter membrane (15) is placed in the first groove, and a pressure ring (20) is detachably connected to the first groove. The pressure ring (20) is in contact with the filter membrane (15), the piston (18) is provided with a first through hole, and a first hard tube (21) is fixedly connected to the first through hole. A first one-way valve (61) is installed on the first hard pipe (21). A second hose (33) is connected to the first hard pipe (21). The second hose (33) is connected to the first hard pipe (21). The filter chamber (12) is connected. 3. A water environment DNA collection device according to claim 2, characterized in that: a plurality of connecting rods (22) are fixedly connected to the inner cylinder (17), and a plurality of connecting rods (22) are fixedly connected to the connecting rod (22). Positioning ring (23), the push-pull rod (19) is located in the positioning ring (23), the push-pull rod (19) is movable in the positioning ring (23), and the push-pull rod (19) is fixed A fixed plate (24) is connected, and a first spring (25) is provided between the fixed plate (24) and the positioning ring (23). The first spring (25) is passed through the push-pull rod (23). 19), the filter press chamber (11) and the filter chamber (12) are both provided with air holes, and a trachea (26) is fixedly connected to the air holes. 4. A water environment DNA collection device according to claim 3, characterized in that: a top block (27) is fixedly connected to the top of the push-pull rod (19), and the top block (27) has a first slope, A supporting plate (28) is fixedly connected to the support column (6), and a push block (29) is fixedly connected to the output end of the electric telescopic rod (16). The push block (29) has a second slope. The push block (29) is slidably installed on the supporting plate (28), and an escape groove is provided on the push block (29). 5. A water environment DNA collection device according to claim 2, characterized in that: the second filtering mechanism includes a filter screen (30) and filter cotton (31), and the bottom shell (9) is provided with a The second groove and the third groove, the filter screen (30) and the filter cotton (31) are detachably connected in the second groove, and the filter cotton (31) is located on the filter screen. (30) above, the side wall of the third groove is provided with threads, the outer edge of the barrel (8) is provided with threads, and the barrel (8) and the third groove are detachably connected through threads , a second through hole is provided on the side wall of the filter cavity (12), a second hard tube (32) is inserted through the second through hole, and the water inlet end of the second hard tube (32) is located at the Above the filter cotton (31), the second flexible tube (33) is fixedly connected to the second hard tube (32). 6. A water environment DNA collection device according to claim 1, characterized in that: the first filtering mechanism includes a filter box (34), and the filter box (34) is fixedly connected to the lower box (34). 1), the filter box (34) is fixedly connected with an interception net (35), the filter box (34) is provided with a water outlet, and the water outlet and the water pump (5) pass through a third hose (36) is connected, the third hose (36) is detachably connected to the water outlet, the output end of the motor (4) is fixedly connected with a rotating shaft (37), and the rotating shaft (37) extends out of the lower The box body (1), the rotating shaft (37) passes through the filter box (34) and the intercepting net (35), the rotating shaft (37) is fixedly connected with a brush (38), and the brush (38) ) is in contact with the interception net (35). 7. A water environment DNA collection device according to claim 1, characterized in that: the turntable (7) is fixedly connected with an installation slot (39), and the bottom shell (9) is detachably connected to the In the installation groove (39), a first cavity (40) is provided in the side wall of the installation groove (39), and a third through hole is provided in the side wall of the first cavity (40). A clamping column (41) is provided in the through hole and the first cavity (40), and a baffle (42) is fixedly connected to the clamping column (41). The baffle (42) is on the third In a cavity (40), a second spring (43) is set on the clamping column (41). The second spring (43) is located in the first cavity (40). The second spring (43) is located in the first cavity (40). One end of the spring (43) is in contact with the baffle (42), the other end of the second spring (43) is in contact with the side wall of the first cavity (40), and there is an opening on the bottom shell (9). There is a clamping slot, the clamping column (41) is detachably connected to the clamping slot, and the end of the clamping column (41) away from the clamping slot is fixedly connected to the hand-operated block (44). 8. A water environment DNA collection device according to claim 7, characterized in that: the lower box (1) is fixedly connected to a number of guide posts (45), and the guide posts (45) are slidingly connected to the outside. There is a movable plate (46), and studs (47) are rotatably connected to the lower box body (1). Threaded holes are provided on the movable plate (46), and the studs (47) are inserted through the In the threaded hole, the stud (47) is movably arranged in the threaded hole, the stud (47) is fixedly connected with a turbine (48), and the lower box (1) is rotatably connected with a worm ( 49), the turbine (48) meshes with the worm (49), a first joint (50) is fixedly connected to the movable plate (46), and the first joint (50) is connected to the first soft The pipe (14) is connected, a through hole (51) is provided in the installation groove (39) and the turntable (7), a second joint is fixedly connected to the water inlet hole (13), and the second joint Plugged into the via hole (51), the first connector (50) is detachably connected to the via hole (51). 9. A water environment DNA collection device according to claim 8, characterized in that: the lower box (1) is fixedly connected to two vertical plates (52), and the worm (49) is rotatably connected to the lower box (1). On the vertical board (52), a number of upright columns (53) are fixedly connected to the lower box body (1). A second cavity is provided in the upright column (53), and a second cavity is provided on the top wall of the second cavity. There is a fourth through hole, and a sphere (54) and a third spring (55) are placed in the second cavity. The sphere (54) extends out of the fourth through hole, and the sphere (54) is connected with the third spring (55). The third spring (55) is in contact, a positioning groove is provided on the turntable (7), and the ball (54) is locked in the positioning groove. 10. A water environment DNA collection device according to claim 6, characterized in that: a plurality of partitions (56) are fixedly connected to the lower box (1), and the partitions (56) connect the The lower box body (1) is divided into the clean water room (2), the motor room (3), the water pump room and the power supply room. The clean water room (2) contains clean water, and the power supply room A power supply (57) is provided in the room. The motor (4) and the electric telescopic rod (16) are electrically connected to the power supply (57). A water adding hole is provided on the side wall of the clean water chamber. The water adding hole is A water adding pipe (58) is fixedly connected inside, and a second one-way valve is installed on the water adding pipe (58). A drainage pipe (59) is provided in the clean water chamber, and the drainage pipe (59) extends out of the lower part. The box body (1), the drainage pipe (59) and the third hose (36) are detachably connected, the top of the support column (6) is fixedly connected with an upper plate (60), and the upper plate (60) ) is fixedly connected with a handle.