CN117327561A - Water environment DNA acquisition facility - Google Patents
Water environment DNA acquisition facility Download PDFInfo
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- CN117327561A CN117327561A CN202311333236.2A CN202311333236A CN117327561A CN 117327561 A CN117327561 A CN 117327561A CN 202311333236 A CN202311333236 A CN 202311333236A CN 117327561 A CN117327561 A CN 117327561A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 150
- 238000001914 filtration Methods 0.000 claims abstract description 42
- 239000012528 membrane Substances 0.000 claims abstract description 23
- 230000007246 mechanism Effects 0.000 claims abstract description 22
- 238000003825 pressing Methods 0.000 claims abstract description 12
- 229920000742 Cotton Polymers 0.000 claims description 14
- 230000006835 compression Effects 0.000 claims description 6
- 238000007906 compression Methods 0.000 claims description 6
- 230000033001 locomotion Effects 0.000 claims description 6
- 210000001503 joint Anatomy 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 210000003437 trachea Anatomy 0.000 claims description 2
- 238000003306 harvesting Methods 0.000 claims 3
- 230000007613 environmental effect Effects 0.000 abstract description 7
- 239000012535 impurity Substances 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses water body environment DNA acquisition equipment, which comprises a lower box body, wherein a clean water chamber, a motor chamber, a water pump chamber and a power supply chamber are arranged in the lower box body, a motor is arranged in the motor chamber, a water pump is arranged in the water pump chamber, a first filtering mechanism is fixedly connected to the lower box body, the water pump is communicated with the first filtering mechanism, a support column is fixedly connected to the outer part of the lower box body, and a rotary table is fixedly connected to the support column; the collecting assembly comprises a filter press, a cylinder body and a bottom shell, wherein the cylinder body is internally and fixedly connected with a transverse plate, the transverse plate divides the cylinder body into a filter pressing cavity and a filter cavity, a second filter mechanism is arranged in the bottom shell, a water inlet hole is formed in the bottom shell, a water pump is communicated with the water inlet hole through a first hose, the first hose is detachably connected with the water inlet hole, the filter press is detachably connected with the cylinder body, the filter press is communicated with the filter cavity, and a filter membrane is arranged in the filter press. The invention can reduce the manual operation condition when collecting the environmental DNA, and can collect a plurality of samples, thereby being more convenient to use.
Description
Technical Field
The invention relates to the field of environmental DNA collection, in particular to water body environmental DNA collection equipment.
Background
Environmental DNA (eDNA) refers to the sum of DNA fragments that can be directly extracted from environmental samples (e.g., water, soil, air, ice core, etc.), and is a mixture of DNA from different species of microorganisms, animals, plants, etc., including both intracellular DNA released into the epidermal cells of the environment by organisms via skin, urine, feces, mucus, etc., and extracellular DNA released into the environment by lysis after cell death. DNA is captured from an environmental sample and stored, extracted, amplified, sequenced and classified to determine the distribution of organisms in the sampling environment.
At present, the eDNA collection mode in the water body is mainly that after a certain volume of water sample to be detected is collected, the water sample is brought back to a laboratory, the experimental staff manually filters and collects the eDNA in the water sample, the filtering process is inconvenient to operate, repeated operation is needed for a plurality of times when a plurality of DNA samples are needed, and the working efficiency is low.
Disclosure of Invention
The invention aims to provide water body environment DNA acquisition equipment so as to solve the problems in the prior art and facilitate the acquisition of environment DNA.
In order to achieve the above object, the present invention provides the following solutions: the invention provides a water environment DNA acquisition device, comprising:
the water pump comprises a lower box body, wherein a clean water chamber, a motor chamber, a water pump chamber and a power supply chamber are arranged in the lower box body, a motor is arranged in the motor chamber, a water pump is arranged in the water pump chamber, a first filtering mechanism is fixedly connected to the lower box body, the water pump is communicated with the first filtering mechanism, a support column is fixedly connected to the outer part of the lower box body, and a rotary table is fixedly connected to the support column;
the collecting assembly comprises a press filter, a cylinder body and a bottom shell, wherein a transverse plate is fixedly connected in the cylinder body, the transverse plate divides the cylinder body into a press filtration cavity and a filter cavity, a second filter mechanism is arranged in the bottom shell, the bottom shell is detachably connected with the cylinder body, the bottom shell is detachably connected with the rotary table, a water inlet hole is formed in the bottom shell, a water pump is communicated with the water inlet hole through a first hose, the first hose is detachably connected with the water inlet hole, the press filter is detachably connected with the cylinder body, the press filter is communicated with the filter cavity, a filter membrane is arranged in the press filter, an electric telescopic rod is fixedly connected to the support column, and the electric telescopic rod is in transmission connection with the press filter.
Preferably, the filter press includes the inner tube, the inner tube can be dismantled and be connected in the barrel, the inner tube is located in the filter-pressing chamber, sliding connection has the piston in the inner tube, fixedly connected with push-and-pull rod on the piston, first recess has been seted up on the inner tube, filter membrane has been placed in the first recess, first recess can be dismantled and be connected with the clamping ring, the clamping ring with filter membrane butt, first through-hole has been seted up on the piston, fixedly connected with first hard tube on the first through-hole, install first check valve on the first hard tube, the intercommunication has the second hose on the first hard tube, the second hose with the filter chamber intercommunication.
Preferably, a plurality of connecting rods are fixedly connected in the inner cylinder, a positioning ring is fixedly connected on the connecting rods, the push-pull rod is positioned on the positioning ring, the push-pull rod is movably arranged in the positioning ring, a fixing plate is fixedly connected on the push-pull rod, a first spring is arranged between the fixing plate and the positioning ring, the first spring penetrates through the push-pull rod, air holes are formed in the filter pressing cavity and the filter cavity, and an air pipe is fixedly connected to the air holes.
Preferably, the push-pull rod top fixedly connected with kicking block, the kicking block has first inclined plane, fixedly connected with layer board on the support column, electric telescopic handle output fixedly connected with ejector pad, the ejector pad has the second inclined plane, the ejector pad is in slide setting on the layer board, dodge the groove has been seted up on the ejector pad.
Preferably, the second filtering mechanism comprises a filter screen and filter cotton, a second groove and a third groove are formed in the bottom shell, the filter screen and the filter cotton are detachably connected in the second groove, the filter cotton is located above the filter screen, threads are formed in the side wall of the third groove, threads are formed in the outer edge of the cylinder, the cylinder and the third groove are detachably connected through the threads, a second through hole is formed in the side wall of the filter cavity, a second hard tube is arranged in the second through hole in a penetrating mode, the water inlet end of the second hard tube is located above the filter cotton, and the second hose is fixedly connected with the second hard tube.
Preferably, the first filtering mechanism comprises a filtering box, the filtering box is fixedly connected to the lower box body, an interception net is fixedly connected to the filtering box, a water outlet hole is formed in the filtering box, the water outlet hole is communicated with the water pump through a third hose, the third hose is detachably connected with the water outlet hole, a rotating shaft is fixedly connected to the output end of the motor, the rotating shaft extends out of the lower box body, the rotating shaft penetrates through the filtering box and the interception net, a hairbrush is fixedly connected to the rotating shaft, and the hairbrush is in butt joint with the interception net.
Preferably, the rotary table is fixedly connected with a mounting groove, the bottom shell is detachably connected in the mounting groove, a first cavity is formed in the side wall of the mounting groove, a third through hole is formed in the side wall of the first cavity, a clamping column is arranged in the first cavity in a penetrating mode, a baffle is fixedly connected to the clamping column, the baffle is arranged in the first cavity, a second spring is sleeved on the clamping column, the second spring is located in the first cavity, one end of the second spring is in butt joint with the baffle, the other end of the second spring is in butt joint with the side wall of the first cavity, a clamping groove is formed in the bottom shell, the clamping column is detachably connected with the clamping groove, and one end of the clamping groove is fixedly connected with a hand pulling block.
Preferably, the lower box body is fixedly connected with a plurality of guide posts, sliding connection has the fly leaf on the guide post, rotate on the lower box body and be connected with the double-screw bolt, set up threaded hole on the fly leaf, the double-screw bolt wears to establish threaded hole, the double-screw bolt is in threaded hole activity sets up, fixedly connected with turbine on the double-screw bolt, it is connected with the worm to rotate on the lower box body, the turbine with the worm meshing, fixedly connected with first joint on the fly leaf, first joint with first hose intercommunication, the mounting groove with the via hole has been seted up on the carousel, fixedly connected with second joint on the inlet port, the second connects to peg graft in the via hole, first joint with the via hole can dismantle and be connected.
Preferably, the lower box body is fixedly connected with two vertical plates, the worm is rotationally connected to the vertical plates, a plurality of stand columns are fixedly connected to the lower box body, a second cavity is formed in the stand columns, a fourth through hole is formed in the top wall of the second cavity, a ball body and a third spring are placed in the second cavity, the ball body extends out of the fourth through hole, the ball body is in butt joint with the third spring, a positioning groove is formed in the rotary disc, and the ball body is clamped in the positioning groove.
Preferably, a plurality of baffles are fixedly connected with the lower box body, the baffles divide the lower box body into the clear water chamber, the motor chamber, the water pump chamber and the power supply chamber, clean water is arranged in the clear water chamber, a power supply is arranged in the power supply chamber, the motor and the electric telescopic rod are electrically connected with the power supply, a water adding hole is formed in the side wall of the clear water chamber, a water adding pipe is fixedly connected with the water adding hole, a second one-way valve is arranged on the water adding pipe, a drain pipe is arranged in the clear water chamber, the drain pipe stretches out of the lower box body, the drain pipe is detachably connected with a third hose, an upper plate is fixedly connected with the top end of the support column, and a handle is fixedly connected with the upper plate.
The invention discloses the following technical effects: in this device, first filtering mechanism carries out the first filtration to water, the water pump is with the water pump after the first filtration to in the drain pan, filter membrane in the filter press is used for filtering the DNA in the water, the barrel is used for connecting filter press and drain pan, second filtering mechanism in the drain pan carries out the secondary to water and filters, the water after the second filters is used for extracting the DNA in the water, electric telescopic handle drives filter press motion, be used for inhaling with filter press the water after the secondary filtration, collection subassembly is the multiunit, after using a set of, rotatable carousel, continue to use another group, still can take off the collection subassembly that has used from the carousel. The invention can reduce the manual operation condition when collecting the environmental DNA, and can collect a plurality of samples, thereby being more convenient to use.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a water environment DNA collection device according to the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1;
FIG. 3 is an enlarged view of a portion of b of FIG. 1;
FIG. 4 is an enlarged view of part of c of FIG. 1;
FIG. 5 is an enlarged view of part of d in FIG. 1;
wherein, 1, lower box body; 2. a clean water chamber; 3. a motor chamber; 4. a motor; 5. a water pump; 6. a support column; 7. a turntable; 8. a cylinder; 9. a bottom case; 10. a cross plate; 11. a filter pressing cavity; 12. a filter chamber; 13. a water inlet hole; 14. a first hose; 15. a filter membrane; 16. an electric telescopic rod; 17. an inner cylinder; 18. a piston; 19. a push-pull rod; 20. a compression ring; 21. a first hard tube; 22. a connecting rod; 23. a positioning ring; 24. a fixing plate; 25. a first spring; 26. an air pipe; 27. a top block; 28. a supporting plate; 29. a pushing block; 30. a filter screen; 31. filtering cotton; 32. a second hard tube; 33. a second hose; 34. a filter box; 35. an interception net; 36. a third hose; 37. a rotating shaft; 38. a brush; 39. a mounting groove; 40. a first cavity; 41. a clamping column; 42. a baffle; 43. a second spring; 44. a hand pulling block; 45. a guide post; 46. a movable plate; 47. a stud; 48. a turbine; 49. a worm; 50. a first joint; 51. a via hole; 52. a vertical plate; 53. a column; 54. a sphere; 55. a third spring; 56. a partition plate; 57. a power supply; 58. a water supply pipe; 59. a drain pipe; 60. an upper plate; 61. a first one-way valve.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Referring to fig. 1-5, the present invention provides a water environment DNA collection apparatus comprising:
the lower box body 1, a clean water chamber 2, a motor chamber 3, a water pump chamber and a power supply chamber are arranged in the lower box body 1, a motor 4 is arranged in the motor chamber 3, a water pump 5 is arranged in the water pump chamber, a first filtering mechanism is fixedly connected to the lower box body 1, the water pump 5 is communicated with the first filtering mechanism, a support column 6 is fixedly connected to the outer part of the lower box body 1, and a rotary table 7 is fixedly connected to the support column 6;
the collection subassembly, collection subassembly is a plurality of groups, collection subassembly includes the filter press, barrel 8 and drain pan 9, fixedly connected with diaphragm 10 in the barrel 8, diaphragm 10 divide into filter-pressing chamber 11 and filter chamber 12 with barrel 8, be provided with second filter mechanism in the drain pan 9, drain pan 9 can dismantle with barrel 8 and be connected, drain pan 9 can dismantle with carousel 7 and be connected, inlet opening 13 has been seted up on the drain pan 9, water pump 5 and inlet opening 13 pass through first hose 14 intercommunication, first hose 14 can dismantle with inlet opening 13 and be connected, the filter press can dismantle with barrel 8 and be connected, filter press and filter chamber 12 intercommunication are provided with filter membrane 15 in the filter press, fixedly connected with electric telescopic handle 16 on the support column 6, electric telescopic handle 16 is connected with the filter press transmission.
The first filtering mechanism filters water for the first time, the water pump 5 pumps the water after the first filtering into the bottom shell 9, the filter membrane 15 in the pressure filter is used for filtering DNA in the water, the barrel 8 is used for connecting the pressure filter and the bottom shell 9, the second filtering mechanism in the bottom shell 9 filters the water for the second time, the water after the second filtering is used for extracting the DNA in the water, the electric telescopic rod 16 drives the pressure filter to move and is used for sucking and filter-pressing the water after the second filtering, the collecting components are multiple groups, when one group is used, the rotary table 7 can be rotated, the other group is continuously used, and the used collecting components can be taken down from the rotary table 7.
Further optimizing scheme, the pressure filter includes inner tube 17, inner tube 17 detachable connection is in barrel 8, inner tube 17 is located filter-pressing chamber 11, sliding connection has piston 18 in the inner tube 17, fixedly connected with push-and-pull rod 19 on the piston 18, first recess has been seted up on the inner tube 17, filter membrane 15 has been placed in the first recess, first recess detachable connection has clamping ring 20, clamping ring 20 and filter membrane 15 butt, first through-hole has been seted up on the piston 18, fixedly connected with first hard tube 21 on the first through-hole, install first check valve 61 on the first hard tube 21, the intercommunication has second hose 33 on the first hard tube 21, second hose 33 and filter chamber 12 intercommunication.
The inner barrel 17 and the barrel 8 are connected and detached through threads, the push-pull rod 19 is used for driving the piston 18 to move, the compression ring 20 is used for pressing the filter membrane 15, the compression ring 20 is connected with the first groove through threads, the first hard tube 21 is detachably connected with the second flexible tube 33, the electric telescopic rod 16 drives the push-pull rod 19 to move, when the push-pull rod 19 ascends, the piston 18 ascends, a gap between the piston 18 and the filter membrane 15 becomes large, water after secondary filtration can be sucked in, when the push-pull rod 19 descends, water inside can be extruded, meanwhile, the first one-way valve 61 can prevent water from flowing out, and after the filter membrane 15 is used, the compression ring 20 can be detached first, and then the filter membrane 15 is taken down.
Further optimizing scheme, fixedly connected with a plurality of connecting rods 22 in the inner tube 17, fixedly connected with holding ring 23 on the connecting rod 22, push-and-pull rod 19 are located holding ring 23, push-and-pull rod 19 is at holding ring 23 internal motion setting, fixedly connected with fixed plate 24 on the push-and-pull rod 19, is provided with first spring 25 between fixed plate 24 and the holding ring 23, and first spring 25 wears to establish on push-and-pull rod 19, has all offered the gas pocket on filter-pressing chamber 11 and the filter chamber 12, fixedly connected with trachea 26 on the gas pocket.
The positioning ring 23 is used for stabilizing the push-pull rod 19, when the electric telescopic rod 16 drives the push-pull rod 19 to move upwards, the fixing plate 24 also moves upwards and compresses the first spring 25, and when the electric telescopic rod 16 leaves the push-pull rod 19, under the action of the first spring 25, the fixing plate 24 drives the push-pull rod 19 to move downwards, so that the inhaled water is extruded, the filtration of the filter membrane 15 on the water can be quickened, the air holes conveniently discharge the air in the filter pressing cavity 11 and the filter cavity 12, and the water is convenient to enter and flow out.
Further optimizing scheme, push-and-pull rod 19 top fixedly connected with kicking block 27, kicking block 27 have first inclined plane, fixedly connected with layer board 28 on the support column 6, electric telescopic handle 16 output fixedly connected with ejector pad 29, ejector pad 29 have the second inclined plane, and ejector pad 29 slides on layer board 28 and sets up, has seted up on the ejector pad 29 and has dodged the groove.
The electric telescopic rod 16 drives the push block 29 to move, when the push block 29 is in contact with the ejector block 27, the second inclined surface of the push block 29 is in contact with the first inclined surface of the ejector block 27, and the ejector block 27 can be jacked up along with the movement of the push block 29, so that the push-pull rod 19 is driven to move upwards, and the purpose of avoiding the groove is to avoid the push-pull rod 19, so that the movement of the push block 29 is prevented from being blocked.
Further optimizing scheme, second filter mechanism includes filter screen 30 and filter pulp 31, second recess and third recess have been seted up in the drain pan 9, filter screen 30 and filter pulp 31 all can dismantle the connection in the second recess, filter pulp 31 is located filter screen 30 top, set up the screw thread on the third recess lateral wall, the screw thread has been seted up to barrel 8 outer fringe, barrel 8 and third recess pass through the screw thread and can dismantle the connection, set up the second through-hole on the filter chamber 12 lateral wall, wear to be equipped with second hard tube 32 in the second through-hole, second hard tube 32 water inlet end is located filter pulp 31 top, second hose 33 and second hard tube 32 fixed connection.
The filter screen 30 and the filter cotton 31 carry out the secondary to the water sample and filter, prevent that impurity in the water from influencing the collection of DNA, filter screen 30 and filter cotton 31 joint are in the second recess, and the convenience is changed filter screen 30 and filter cotton 31, and barrel 8 and drain pan 9 pass through threaded connection, and the installation is more convenient with dismantling, and second hard tube 32 and second hose 33 are connected, make things convenient for the connection and the dismantlement of second hose 33.
Further optimizing scheme, first filter mechanism includes filter box 34, filter box 34 fixed connection is on lower box body 1, fixedly connected with interception net 35 on the filter box 34, the apopore has been seted up on the filter box 34, the apopore passes through third hose 36 intercommunication with water pump 5, third hose 36 is connected with the apopore can be dismantled, motor 4 output fixedly connected with pivot 37, lower box body 1 is stretched out to pivot 37, filter box 34 and interception net 35 are passed to pivot 37, fixedly connected with brush 38 in the pivot 37, brush 38 and interception net 35 butt.
The interception net 35 is used for intercepting larger impurities, the third hose 36 is used for connecting the filter box 34 and the water pump 5, the motor 4 drives the rotating shaft 37 to rotate, the rotating shaft 37 drives the hairbrush 38 to rotate, and the hairbrush 38 can brush the impurities on the interception net 35 to prevent the interception net 35 from being blocked.
Further optimizing scheme, fixedly connected with mounting groove 39 on the carousel 7, the drain pan 9 can dismantle the connection in mounting groove 39, first cavity 40 has been seted up in the mounting groove 39 lateral wall, the third through-hole has been seted up on the first cavity 40 lateral wall, wear to be equipped with in third through-hole and the first cavity 40 and block the post 41, fixedly connected with baffle 42 on the post 41, baffle 42 is in first cavity 40, the cover is equipped with second spring 43 on the post 41, second spring 43 is located first cavity 40, second spring 43 one end and baffle 42 butt, second spring 43 other end and first cavity 40 lateral wall butt have been seted up the draw-in groove on the drain pan 9, block the post 41 and can dismantle the connection with the draw-in groove, block 41 is kept away from draw-in groove one end fixed connection hand piece 44.
The bottom shell 9 can be dismantled and connected in the mounting groove 39, when the bottom shell 9 is installed, outwards stimulate the hand piece 44, the hand piece 44 drives the card post 41 and removes, in the card post 41 gets into the third through hole completely, can put into the mounting groove 39 with the bottom shell 9, when card post 41 removes, can drive baffle 42 and remove, baffle 42 removes and can compress second spring 43, unclamp behind the hand piece 44, under the effect of second spring 43, the end of card post 41 can get into the draw-in groove to with bottom shell 9 card post.
According to a further optimization scheme, a plurality of guide posts 45 are fixedly connected to the outer portion of the lower box body 1, a movable plate 46 is connected to the guide posts 45 in a sliding mode, a stud 47 is connected to the lower box body 1 in a rotating mode, a threaded hole is formed in the movable plate 46, the stud 47 penetrates through the threaded hole, the stud 47 is movably arranged in the threaded hole, a turbine 48 is fixedly connected to the stud 47, a worm 49 is connected to the lower box body 1 in a rotating mode, the turbine 48 is meshed with the worm 49, a first connector 50 is fixedly connected to the movable plate 46, the first connector 50 is communicated with the first hose 14, a through hole 51 is formed in the mounting groove 39 and the turntable 7, a second connector is fixedly connected to the water inlet 13 and is inserted into the through hole 51, the first connector 50 is detachably connected with the through hole 51, and a blocking block is fixedly connected to the top end of the guide posts 45.
When the bottom shell 9 is placed in the mounting groove 39, the second connector is inserted into the through hole 51, the movable plate 46 drives the first connector 50 to move, the first connector 50 is used for being connected with the through hole 51, during connection, the worm 49 is rotated, the worm 49 drives the turbine 48 to rotate, the turbine 48 drives the stud 47 to rotate, the stud 47 drives the movable plate 46 to move up and down, the stud 47 is rotationally connected with the lower box body 1, when the stud 47 rotates, the stud 47 does not move on the lower box body 1, and when the turntable 7 rotates, the second connector and the through hole 51 need to be disassembled, and the worm 49 is reversely rotated.
According to a further optimization scheme, two vertical plates 52 are fixedly connected to the outer portion of the lower box body 1, a worm 49 is rotatably connected to the vertical plates 52, a plurality of upright posts 53 are fixedly connected to the lower box body 1, a second cavity is formed in each upright post 53, a fourth through hole is formed in the top wall of each second cavity, a ball 54 and a third spring 55 are placed in each second cavity, the ball 54 extends out of the fourth through hole, the ball 54 is abutted to the third spring 55, a positioning groove is formed in the rotary disc 7, and the ball 54 is clamped in the positioning groove.
The vertical plate 52 is used for supporting the worm 49, the ball 54 is used for being abutted with the turntable 7, when the turntable 7 is rotated, the ball 54 can move on the surface of the turntable 7, after the ball 54 enters the positioning groove, the turntable 7 can be clamped, the turntable 7 is prevented from rotating, and the ball 54 can leave the positioning groove by forcefully rotating the turntable 7.
Further optimizing scheme, a plurality of baffles 56 of fixedly connected with in the lower box body 1, baffle 56 will be separated into clean water room 2, motor room 3, water pump 5 room and power room in the lower box body 1, clean water is equipped with in the clean water room 2, be provided with power 57 in the power 57 room, motor 4 and electric telescopic handle 16 and power 57 electric connection, the water hole has been seted up on the clean water room lateral wall, water hole internal fixation has water pipe 58, install the second check valve on the water pipe 58, be provided with the drain pipe in the clean water room, the drain pipe stretches out box body 1 down, the drain pipe can dismantle with third hose 36 and be connected, support column 6 top fixedly connected with upper plate 60, upper plate 60 fixedly connected with handle.
The baffle 56 is used for separating clean water room 2, motor room 3, water pump 5 room and power 57 room, clean water is used for washing third hose 36, water pump 5 and first hose 14, after a set of filter press sampling is accomplished, take out this device from water, take off drain pan 9 from carousel 7, then connect third hose 36 on the drain pipe of clean water room, then start water pump 5, wash the pipeline, prevent to influence the collection next time, water pipe 58 is convenient to add water to clean water indoor.
According to the use method of the device, the device is placed into water in a target area, then the device is placed under water, the water enters a filter box 34, an interception net 35 is used for carrying out first filtering on the water, after a water pump 5 is started, the water pump 5 is used for sending the water into a bottom shell 9 through a first hose 14, a filter screen 30 and filter cotton 31 are arranged in the bottom shell 9, the filter screen 30 and the filter cotton 31 carry out second filtering on a water sample, an electric telescopic rod 16 is started, the electric telescopic rod 16 drives a push block 29 to move, when the push block 29 is in contact with the push block 27, a second inclined surface of the push block 29 is in contact with the first inclined surface of the push block 27, the push block 27 is jacked up along with the movement of the push block 29, so that a push rod 19 is driven to move upwards, a fixed plate 24 is also driven to move upwards, a first spring 25 is compressed, when the push rod 19 is lifted up, a gap between the piston 18 and a filter membrane 15 is increased, the water enters between the piston 18 and the piston 15 through a second hard tube 32, the second hose 33 and the first hard tube 21, after the water enters the push block 29, the electric telescopic rod 16 is contacted with the first inclined surface of the push block 29, the push block 27 is separated from the first inclined surface, the first inclined surface of the push block 27 is driven to move towards the filter membrane 19, and the filter membrane is driven to be sucked down by the filter membrane 25, and the filter membrane is fixed. When the sampling of the group of press filters is completed, the device is taken out from water, the bottom shell 9 is taken off from the rotary table 7, the worm 49 is reversely rotated, the worm 49 drives the turbine 48 to rotate, the turbine 48 drives the stud 47 to rotate, the movable plate 46 moves downwards, the second connector and the through hole 51 are disassembled, then the rotary table 7 is rotated, and the other group of press filters, the cylinder 8 and the bottom shell 9 are replaced.
In the description of the present invention, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (10)
1. A water environment DNA collection apparatus comprising:
the novel water purifier comprises a lower box body (1), wherein a clean water chamber (2), a motor chamber (3), a water pump chamber and a power supply chamber are arranged in the lower box body (1), a motor (4) is arranged in the motor chamber (3), a water pump (5) is arranged in the water pump chamber, a first filtering mechanism is fixedly connected to the lower box body (1), the water pump (5) is communicated with the first filtering mechanism, a support column (6) is fixedly connected to the outer part of the lower box body (1), and a rotary table (7) is fixedly connected to the support column (6);
the collecting assembly is a plurality of groups, the collecting assembly comprises a press filter, a barrel (8) and a bottom shell (9), a cross plate (10) is fixedly connected in the barrel (8), the cross plate (10) is used for dividing the barrel (8) into a press filtration cavity (11) and a filter cavity (12), a second filter mechanism is arranged in the bottom shell (9), the bottom shell (9) is detachably connected with the barrel (8), the bottom shell (9) is detachably connected with the rotary disc (7), a water inlet hole (13) is formed in the bottom shell (9), the water pump (5) is communicated with the water inlet hole (13) through a first hose (14), the press filter is detachably connected with the barrel (8), the press filter is communicated with the filter cavity (12), a filter membrane (15) is arranged in the press filter, an electric rod (16) is fixedly connected with the support column (6), and the electric rod (16) is connected with the telescopic filter.
2. The water environment DNA collection apparatus of claim 1, wherein: the filter press comprises an inner cylinder (17), the inner cylinder (17) is detachably connected in the cylinder body (8), the inner cylinder (17) is located in a filter pressing cavity (11), a piston (18) is connected in the inner cylinder (17) in a sliding mode, a push-pull rod (19) is fixedly connected to the piston (18), a first groove is formed in the inner cylinder (17), a filter membrane (15) is placed in the first groove, a compression ring (20) is detachably connected to the first groove, the compression ring (20) is abutted to the filter membrane (15), a first through hole is formed in the piston (18), a first hard pipe (21) is fixedly connected to the first through hole, a first one-way valve (61) is installed on the first hard pipe (21), a second hose (33) is communicated to the first hard pipe (21), and the second hose (33) is communicated with the filter cavity (12).
3. A water environment DNA harvesting apparatus according to claim 2, wherein: fixedly connected with a plurality of connecting rods (22) in inner tube (17), fixedly connected with holding ring (23) on connecting rod (22), push-and-pull rod (19) are located holding ring (23), push-and-pull rod (19) are in holding ring (23) internal motion sets up, fixedly connected with fixed plate (24) on push-and-pull rod (19), fixed plate (24) with be provided with first spring (25) between holding ring (23), first spring (25) wear to establish on push-and-pull rod (19), filter-pressing chamber (11) with all offer the gas pocket on filter chamber (12), fixedly connected with trachea (26) on the gas pocket.
4. A water environment DNA harvesting apparatus according to claim 3, wherein: push-and-pull rod (19) top fixedly connected with kicking block (27), kicking block (27) have first inclined plane, fixedly connected with layer board (28) on support column (6), electric telescopic handle (16) output fixedly connected with ejector pad (29), ejector pad (29) have the second inclined plane, ejector pad (29) are in slide setting on layer board (28), dodge the groove has been seted up on ejector pad (29).
5. A water environment DNA harvesting apparatus according to claim 2, wherein: the second filtering mechanism comprises a filter screen (30) and filter cotton (31), a second groove and a third groove are formed in the bottom shell (9), the filter screen (30) and the filter cotton (31) are detachably connected in the second groove, the filter cotton (31) is located above the filter screen (30), threads are formed in the side wall of the third groove, threads are formed in the outer edge of the cylinder body (8), the cylinder body (8) and the third groove are detachably connected through the threads, a second through hole is formed in the side wall of the filter cavity (12), a second hard tube (32) is arranged in the second through hole in a penetrating mode, and the water inlet end of the second hard tube (32) is located above the filter cotton (31), and the second hose (33) is fixedly connected with the second hard tube (32).
6. The water environment DNA collection apparatus of claim 1, wherein: the first filtering mechanism comprises a filtering box (34), the filtering box (34) is fixedly connected to the lower box body (1), an interception net (35) is fixedly connected to the filtering box (34), a water outlet is formed in the filtering box (34), the water outlet is communicated with a water pump (5) through a third hose (36), the third hose (36) is detachably connected with the water outlet, a rotating shaft (37) is fixedly connected to the output end of the motor (4), the rotating shaft (37) extends out of the lower box body (1), a brush (38) is fixedly connected to the rotating shaft (37) through the filtering box (34) and the interception net (35), and the brush (38) is in butt with the interception net (35).
7. The water environment DNA collection apparatus of claim 1, wherein: fixedly connected with mounting groove (39) on carousel (7), connect can be dismantled in mounting groove (39) bottom shell (9), first cavity (40) have been seted up in mounting groove (39) lateral wall, the third through-hole has been seted up on first cavity (40) lateral wall, the third through-hole with wear to be equipped with clamping column (41) in first cavity (40), fixedly connected with baffle (42) on clamping column (41), baffle (42) are in first cavity (40), the cover is equipped with second spring (43) on clamping column (41), second spring (43) are located in first cavity (40), second spring (43) one end with baffle (42) butt, the second spring (43) other end with first cavity (40) lateral wall butt has been seted up on bottom shell (9), clamping column (41) with clamping groove can dismantle to be connected, clamping column (41) are kept away from clamping block (44) fixed connection hand piece one end.
8. The water environment DNA collection apparatus of claim 7, wherein: the utility model discloses a box body, including box body (1) and movable plate (46) including lower box body (1), box body (1) and movable plate (46) are gone up to lower box body (45) external fixation, sliding connection has movable plate (46) on guide pillar (45), the last screw hole that has seted up of movable plate (46), screw bolt (47) are worn to establish screw hole, screw bolt (47) are in screw hole activity sets up, fixedly connected with turbine (48) on screw bolt (47), it is connected with worm (49) to go up to rotate on box body (1), turbine (48) with worm (49) meshing, fixedly connected with first joint (50) on movable plate (46), first joint (50) with first hose (14) intercommunication, mounting groove (39) with set up via hole (51) on carousel (7), fixedly connected with second joint on inlet port (13) is pegged graft in via hole (51), first joint (50) with can dismantle via hole (51).
9. The water environment DNA collection apparatus of claim 8, wherein: the novel box comprises a box body (1), wherein two vertical plates (52) are fixedly connected to the outside of the box body (1), a worm (49) is rotatably connected to the vertical plates (52), a plurality of vertical columns (53) are fixedly connected to the box body (1) below, a second cavity is formed in each vertical column (53), a fourth through hole is formed in the top wall of the second cavity, a ball body (54) and a third spring (55) are placed in the second cavity, the ball body (54) extends out of the fourth through hole, the ball body (54) is in butt joint with the third spring (55), a positioning groove is formed in the rotary disc (7), and the ball body (54) is clamped in the positioning groove.
10. The water environment DNA collection apparatus of claim 6, wherein: the utility model discloses a water-saving device for the solar cell is characterized in that a plurality of baffles (56) are fixedly connected with in a lower box body (1), baffle (56) will divide into in the lower box body (1) clear water room (2) motor room (3) water pump room with the power supply room, clear water room (2) are equipped with clean water, be provided with power supply (57) in the power supply room, motor (4) with electric telescopic handle (16) with power supply (57) electric connection, the water hole has been seted up on the clear water room lateral wall, water pipe (58) are added to fixedly connected with in the water hole, install the second check valve on water pipe (58), be provided with drain pipe (59) in the clear water room, drain pipe (59) stretch out lower box body (1), drain pipe (59) with third hose (36) detachable connection, support column (6) top fixedly connected with upper plate (60), upper plate (60) fixedly connected with handle.
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| CN202311333236.2A CN117327561B (en) | 2023-10-16 | 2023-10-16 | Water environment DNA acquisition facility |
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Cited By (1)
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| CN117568143A (en) * | 2024-01-15 | 2024-02-20 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Deep sea sample nucleic acid in-situ digestion and preservation device |
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| CN117327561B (en) | 2024-07-26 |
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