CN117568143B - Deep sea sample nucleic acid in-situ digestion and preservation device - Google Patents
Deep sea sample nucleic acid in-situ digestion and preservation device Download PDFInfo
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- CN117568143B CN117568143B CN202410053146.6A CN202410053146A CN117568143B CN 117568143 B CN117568143 B CN 117568143B CN 202410053146 A CN202410053146 A CN 202410053146A CN 117568143 B CN117568143 B CN 117568143B
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- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 46
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 46
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 46
- 230000029087 digestion Effects 0.000 title claims abstract description 29
- 238000004321 preservation Methods 0.000 title claims abstract description 26
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 25
- 238000001179 sorption measurement Methods 0.000 claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 239000013535 sea water Substances 0.000 claims abstract description 20
- 238000005336 cracking Methods 0.000 claims abstract description 19
- 244000005700 microbiome Species 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims abstract description 9
- 238000003860 storage Methods 0.000 claims abstract description 9
- 239000012149 elution buffer Substances 0.000 claims abstract description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 16
- 238000007789 sealing Methods 0.000 claims description 15
- 230000002572 peristaltic effect Effects 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 7
- 238000000197 pyrolysis Methods 0.000 claims description 5
- 238000005192 partition Methods 0.000 claims description 4
- 244000309464 bull Species 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 8
- 210000000170 cell membrane Anatomy 0.000 abstract description 6
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- 238000001125 extrusion Methods 0.000 abstract description 2
- 230000000149 penetrating effect Effects 0.000 abstract description 2
- 238000001556 precipitation Methods 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D88/00—Large containers
- B65D88/74—Large containers having means for heating, cooling, aerating or other conditioning of contents
- B65D88/745—Large containers having means for heating, cooling, aerating or other conditioning of contents blowing or injecting heating, cooling or other conditioning fluid inside the container
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of microbiology, and particularly relates to a deep sea sample nucleic acid in-situ digestion and preservation device which comprises a tank body, wherein a sea water pump is fixedly connected to the upper side wall of the tank body, a conveying pipe penetrating and extending into the tank body is fixedly connected to the output end of the sea water pump, two guide rods are fixedly connected to the inner wall of the tank body, sliding plates are sleeved at the end parts of the two guide rods in a sliding manner, a filter membrane is fixedly connected between the end parts of the sliding plates, and a collecting tank is fixedly connected to the inner wall of the tank body. According to the invention, the flow of seawater is promoted by the reciprocating stretching of the filter membrane, the precipitation of microorganisms is accelerated, the rotation and mutual extrusion of the crushing disc are realized by the swinging of the rotating plate when the microorganisms are led into the cracking groove, the primary cell membrane rupture of the microorganisms is realized, the secondary rupture is carried out by the subsequent contact with the cracking liquid, the extraction of nucleic acid is accelerated, and the microorganisms are precipitated in the elution buffer solution for low-temperature storage after the adsorption and extraction, so that the pollution, degradation and loss of samples are avoided.
Description
Technical Field
The invention relates to the technical field of microbiology, in particular to a deep sea sample nucleic acid in-situ digestion and preservation device.
Background
The deep sea environment refers to sea water and seabed sediments and the like at the sea bottom, and the composition and properties of biological and non-biological components in the deep sea environment are greatly different from those of land and shallow sea environments due to special conditions such as high pressure, low temperature, darkness and the like of the deep sea environment. The organisms in the deep sea environment are mostly unique benthic organisms, and the method has important scientific research value. With the continuous deep sea scientific research, the research on the biological communities in the deep sea environment is increasingly important.
Nucleic acids are a very important subject of investigation in the study of deep sea organisms. Currently, widely used nucleic acid extraction methods include mechanical disruption, phenol-chloroform, ion exchange column, magnetic bead, and the like. CN209854124U discloses an in-situ extraction device for nucleic acid of deep sea microorganism, sea water is contacted with a lysate to crack after passing through a filter membrane, but the opening degree of cell membrane of microorganism cannot be ensured, so as to affect the extraction of subsequent nucleic acid.
In order to solve the problems, we propose an in-situ digestion and preservation device for nucleic acid of a deep sea sample.
Disclosure of Invention
The invention aims to solve the problems in the background technology and provides a deep sea sample nucleic acid in-situ digestion and preservation device.
In order to achieve the above purpose, the present invention adopts the following technical scheme: the utility model provides a deep sea sample nucleic acid normal position digestion and save set, includes a jar body, the upper side wall fixedly connected with sea water pump of jar body, the output fixedly connected with of sea water pump runs through the conveyer pipe that extends to the jar internally, the inner wall fixedly connected with two guide bars of jar body, two the tip slip cap of guide bar is equipped with the slide, fixedly connected with filter membrane between the tip of slide, the inner wall fixedly connected with collecting vat of jar body, filter membrane outer wall fixedly connected with row material pipe, the tip of row material pipe is equipped with valve one, the lower extreme of row material pipe is fixed through the collecting vat setting and fixedly connected with digester that is used for broken microorganism, the lower side wall fixedly connected with unloading pipe of digester, the internal fixedly connected with schizolysis tank of jar;
the utility model discloses a liquid nitrogen storage tank, including jar body, lower lateral wall fixedly connected with centrifuge tube, the interior bottom wall fixedly connected with motor of jar body, the lower lateral wall fixedly connected with conveying tube of schizolysis groove, be provided with valve two on the conveying tube, the outer wall connection of centrifuging tube has the pigging and is located the nucleic acid tube of pigging downside, jar internal fixedly connected with preserves jar, be provided with adsorption chamber, dry chamber and low temperature chamber in preserving jar, the last lateral wall of preserving jar is fixed with the liquid nitrogen pipe with low temperature chamber intercommunication, low temperature intracavity is equipped with elution buffer.
In the deep sea sample nucleic acid in-situ digestion and preservation device, a swinging mechanism is connected between the tank body and each sliding plate, the swinging mechanism comprises a gear arranged in the tank body and a rotating plate rotatably arranged in the tank body, a sliding groove is formed in the rotating plate, a second fixing rod and a first fixing rod which are positioned in the sliding groove are fixed on the outer wall of the gear and the outer wall of the sliding plate, the two gears are meshed with each other, a motor is fixedly connected with the inner wall of the tank body, and the driving end of the motor is fixedly connected with the center of a circle of the left gear.
In the deep sea sample nucleic acid in-situ digestion and preservation device, the digester comprises a hollow body, two piston plates are slidably arranged in the hollow body, a motor I is fixedly embedded on each piston plate, two motors I are oppositely arranged, a driving end is fixedly connected with a crushing disc, two ends, away from each other, of the motors I are fixedly connected with a connecting rod penetrating through the inner wall of the hollow body, a rotating rod is rotatably connected between the connecting rod and a rotating plate positioned on the same side, and a telescopic spring is fixedly connected between each piston plate and the inner wall of the hollow body.
In the deep sea sample nucleic acid in-situ digestion and preservation device, a peristaltic pump is fixed in the tank body, and a conveying pipe for conveying a pyrolysis liquid is connected between the output end of the peristaltic pump and the pyrolysis tank.
In the deep sea sample nucleic acid in-situ digestion and preservation device, a plurality of discharge holes are formed in the inner wall of the cracking groove, a cavity communicated with the discharge holes is formed in the cracking groove, and the output end of the peristaltic pump is communicated with the cavity.
In the deep sea sample nucleic acid in-situ digestion and preservation device, the specifications of the adsorption cavity, the drying cavity and the low-temperature cavity are consistent, and the connecting parts are provided with the partition boards for isolation.
In the deep sea sample nucleic acid in-situ digestion and preservation device, the end part of the rotating plate is fixedly connected with the rotating shaft, the end part of the rotating shaft is fixedly sleeved with the bearing, and the bearing is fixed on the inner wall of the tank body.
In the deep sea sample nucleic acid in-situ digestion and preservation device, the motor II is fixedly embedded in the upper side wall of the preservation tank, the driving end of the motor II is vertically and downwards fixedly connected with the electric push rod, the driving end of the electric push rod is vertically and downwards fixedly connected with the sealing plate I and the sealing plate II, and the lower side wall of the sealing plate I is fixedly connected with the adsorption column.
Compared with the prior art, the deep-sea sample nucleic acid in-situ digestion and preservation device has the advantages that:
according to the invention, the flow of seawater is promoted by the reciprocating stretching of the filter membrane, the precipitation of microorganisms is accelerated, the rotation and mutual extrusion of the crushing disc are realized by the swinging of the rotating plate when the microorganisms are led into the cracking groove, the primary cell membrane rupture of the microorganisms is realized, the secondary rupture is carried out by the subsequent contact with the cracking liquid, the extraction of nucleic acid is accelerated, and the microorganisms are precipitated in the elution buffer solution for low-temperature storage after the adsorption and extraction, so that the pollution, degradation and loss of samples are avoided.
Drawings
FIG. 1 is an internal view of a deep sea sample nucleic acid in situ digestion and preservation device according to the present invention;
FIG. 2 is an external view of a deep sea sample nucleic acid in situ digestion and preservation device according to the present invention;
FIG. 3 is an enlarged view of part A of FIG. 1;
FIG. 4 is a schematic diagram showing the internal structure of a digester in a deep sea sample nucleic acid in-situ digestion and preservation device according to the present invention;
fig. 5 is a plan sectional view of a holding tank in a deep sea sample nucleic acid in-situ digestion and holding device according to the present invention.
In the figure: 1. a tank body; 2. a guide rod; 3. a slide plate; 4. a filter membrane; 5. sea water pump; 6. a bearing; 7. a rotating shaft; 8. a rotating plate; 9. a chute; 10. a first fixed rod; 11. a gear; 12. a second fixing rod; 13. a collection tank; 14. a discharge pipe; 15. a digester; 16. a connecting rod; 17. a rotating rod; 18. a piston plate; 19. a first motor; 20. a shredder plate; 21. a telescopic spring; 22. a peristaltic pump; 23. a discharge port; 24. a motor; 25. centrifuging tube; 26. cleaning the pipe; 27. a nucleic acid tube; 28. a holding tank; 29. a second motor; 30. a liquid nitrogen pipe; 31. an adsorption chamber; 32. a low temperature chamber; 33. a drying chamber; 34. a first sealing plate; 35. a second sealing plate; 36. an electric push rod; 37. a cleavage tank.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Referring to fig. 1-5, a deep sea sample nucleic acid in-situ digestion and preservation device comprises a tank body 1, wherein a sea water pump 5 is fixedly connected to the upper side wall of the tank body 1, the output end of the sea water pump 5 is fixedly connected with a conveying pipe which penetrates and extends into the tank body 1, two guide rods 2 are fixedly connected to the inner wall of the tank body 1, sliding plates 3 are sleeved at the end parts of the two guide rods 2 in a sliding manner, a filter membrane 4 is fixedly connected between the end parts of the sliding plates 3, a collecting tank 13 is fixedly connected to the inner wall of the tank body 1, a discharge pipe 14 is fixedly connected to the outer wall of the filter membrane 4, a valve I is arranged at the end part of the discharge pipe 14, the sea water pump 5 pumps sea water into the filter membrane 4 for filtering, the guide rods 2 play a role in guiding the sliding plates 3, and the linear motion of the sliding plates 3 is guaranteed all the time.
A swinging mechanism is connected between the tank body 1 and each sliding plate 3, the swinging mechanism comprises a gear 11 arranged in the tank body 1 and a rotating plate 8 rotatably arranged in the tank body 1, the end part of the rotating plate 8 is fixedly connected with a rotating shaft 7, the end part of the rotating shaft 7 is fixedly sleeved with a bearing 6, the bearing 6 is fixed on the inner wall of the tank body 1, a sliding groove 9 is formed in the rotating plate 8, the outer wall of the gear 11 and the outer wall of the sliding plate 3 are fixedly provided with a fixing rod two 12 and a fixing rod one 10 which are positioned in the sliding groove 9, the two gears 11 are meshed with each other, the inner wall of the tank body 1 is fixedly connected with a motor, the driving end of the motor is fixedly connected with the center of the gear 11 at the left side, the seawater pump 5 pumps the seawater into a conveying pipe, and then flows out from the end part of the conveying pipe into a filter membrane 4, in the process of filtering seawater by the filter membrane 4, the motor is started to drive the gear 11 to rotate, because the two gears 11 are meshed with each other, when the gears 11 drive the fixing rods II 12 to rotate outwards, the fixing rods II 12 push the rotary plates 8 to rotate outwards, meanwhile, the upper ends of the rotary plates 8 push the fixing rods I10 and the sliding plates 3 to move outwards, the two sliding plates 3 move outwards simultaneously, the filter membrane 4 is pulled towards two sides from a downward collapsed state, when the gears 11 drive the fixing rods II 12 to rotate inwards, the fixing rods II 12 push the rotary plates 8 to rotate inwards, at the moment, the upper ends of the rotary plates 8 push the sliding plates 3 to move inwards, the end parts of the filter membrane 4 are pushed to move inwards, so that the filter membrane 4 is converted to a collapsed state, the flow of seawater in the filter membrane 4 is realized through the reciprocating stretching and collapsing of the filter membrane 4, the filtering of the seawater is promoted, and microorganisms are quickly precipitated and remained in the filter membrane 4.
Further, the second fixing rod 12 is arranged offset from the center of the gear 11. The lower extreme of row material pipe 14 is fixed and is run through collecting vat 13 setting and fixedly connected with is used for the digestion ware 15 of broken microorganism, the lower lateral wall fixedly connected with discharge tube of digestion ware 15, the digestion ware 15 includes the hollow ware body, the internal slip of hollow ware is provided with two piston plates 18, every piston plate 18 is last to be fixedly inlayed and is equipped with motor one 19, two motor one 19 set up relatively, and drive end fixedly connected with crushing dish 20, the one end that two motor one 19 kept away from all is fixedly connected with link 16 that runs through the setting of hollow ware body inner wall, rotate between link 16 and the rotating plate 8 that is located same one side and be connected with bull stick 17, fixedly connected with expansion spring 21 between the inner wall of two piston plates 18 and the hollow ware body, open valve one, the microorganism that filters out is led out through row material pipe 14, simultaneously motor one 19 drives crushing dish 20 and rotates, gear 11 drives dead lever two 12 rotations, when pushing rotating plate 8 and swinging to the outside, through bull stick 17 pulling link 16 and 18 outside and piston plate 8 moves, when rotating plate 8 swings to the inside, reverse elasticity of expansion spring 21 makes down and moves down to the same cell membrane and the mutual microorganism is broken, the cell membrane is broken down, and the microorganism is avoided moving to the inside to the membrane is broken.
The tank body 1 is fixedly connected with a cracking groove 37, the peristaltic pump 22 is fixedly arranged in the tank body 1, a conveying pipe for conveying cracking liquid is connected between the output end of the peristaltic pump 22 and the cracking groove 37, a plurality of discharge holes 23 are formed in the inner wall of the cracking groove 37, a cavity communicated with the discharge holes 23 is formed in the cracking groove 37, the output end of the peristaltic pump 22 is communicated with the cavity, the smashing disc 20 is used for downwards flowing into the cracking groove 37 after cracking microorganisms, the cracking liquid in the tank body 1 is guided into the cracking groove 37 through the peristaltic pump 22, cell membranes of the microorganisms are further damaged, nucleic acid is released into the solution, and initial extraction of the nucleic acid is achieved.
The inner bottom wall of the tank body 1 is fixedly connected with a motor 24, the driving end of the motor 24 is fixedly connected with a centrifuge tube 25, the lower side wall of a cracking groove 37 is fixedly connected with a feeding tube, a valve II is arranged on the feeding tube, the outer wall of the centrifuge tube 25 is connected with an upper cleaning tube 26 and a nucleic acid tube 27 positioned at the lower side of the upper cleaning tube 26, the tank body 1 is fixedly connected with a storage tank 28, an adsorption cavity 31, a drying cavity 33 and a low-temperature cavity 32 are arranged in the storage tank 28, the sizes of the adsorption cavity 31, the drying cavity 33 and the low-temperature cavity 32 are consistent, partition plates for isolation are arranged at the joints, the partition plates are arranged to avoid or reduce temperature transmission, a motor II 29 is fixedly embedded in the upper side wall of the storage tank 28, an electric push rod 36 is vertically and fixedly connected with a sealing plate I34 and a sealing plate II 35 at the driving end of the electric push rod 36, the lower side wall of the first sealing plate 34 is fixedly connected with an adsorption column, the upper side wall of the preservation tank 28 is fixedly provided with a liquid nitrogen pipe 30 communicated with a low-temperature cavity 32, an elution buffer is arranged in the low-temperature cavity 32, after nucleic acid is initially extracted, a valve II is opened to guide out the solution into a centrifuge tube 25, the centrifuge tube 25 is driven by a motor 24 to rotate, the solution is centrifugally moved, substances such as protein are separated out of supernatant liquid, the subsequent adsorption column is prevented from being blocked by solidification of the protein, nucleic acid is separated out at the lower layer, the supernatant liquid is guided out by an upper cleaning tube 26 at the moment, the nucleic acid is guided out into an adsorption cavity 31 by a nucleic acid pipe 27 (a valve III, a valve IV and other control flow components can be respectively arranged on the upper cleaning tube 26 and the nucleic acid pipe 27), the motor II 29 drives an electric push rod 36 and the first sealing plate 34 and the second sealing plate 35 to rotate to enable the adsorption column to rotate to the upper side of the adsorption cavity 31, the driving end of the electric push rod 36 extends out to enable the adsorption column to be inserted into the adsorption cavity 31 for adsorbing and extracting nucleic acid;
after the adsorption is finished, the driving end of the electric push rod 36 is retracted and continuously rotates until the adsorption column is positioned in the low-temperature cavity 32, the electric push rod 36 pushes down the adsorption column to be in contact with the elution buffer solution, nucleic acid is precipitated into the elution buffer solution, then the electric push rod 36 is retracted again and continuously rotates until the adsorption column is positioned on the upper side of the drying cavity 33, the driving end of the electric push rod 36 extends out to enable the first sealing plate 34 and the second sealing plate 35 to move downwards to seal the drying cavity 33 and the low-temperature cavity 32, and liquid nitrogen is led into the low-temperature cavity 32 through the liquid nitrogen pipe 30, so that the elution buffer solution containing the nucleic acid is stored at a low temperature.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (6)
1. The utility model provides a deep sea sample nucleic acid normal position digestion and save set, includes a jar body (1), its characterized in that, the upper side wall fixedly connected with sea water pump (5) of jar body (1), the output fixedly connected with of sea water pump (5) runs through the conveyer pipe that extends to in jar body (1), the inner wall fixedly connected with two guide bars (2) of jar body (1), two the tip slip cap of guide bar (2) is equipped with slide (3), fixedly connected with filter membrane (4) between the tip of slide (3), the inner wall fixedly connected with collecting vat (13) of jar body (1), filter membrane (4) outer wall fixedly connected with row material pipe (14), the tip of row material pipe (14) is equipped with valve one, the lower extreme fixedly connected with of row material pipe (14) runs through collecting vat (13) setting and fixedly connected with digester (15) that are used for breaking microorganism, the lower lateral wall fixedly connected with of digester (15) is connected with unloading pipe, the inside the jar body (1) is fixedly connected with pyrolysis tank (37);
the utility model discloses a liquid nitrogen buffer solution tank, including a tank body (1), a motor (24) is fixedly connected with the interior bottom wall of the tank body (1), a centrifuge tube (25) is fixedly connected with the drive end of the motor (24), a feed pipe is fixedly connected with the lower side wall of a cracking groove (37), a valve II is arranged on the feed pipe, an upper pipe cleaning (26) and a nucleic acid tube (27) positioned at the lower side of the upper pipe cleaning (26) are connected with the outer wall of the centrifuge tube (25), a storage tank (28) is fixedly connected with the tank body (1), an adsorption cavity (31), a drying cavity (33) and a low-temperature cavity (32) are arranged in the storage tank (28), a liquid nitrogen tube (30) communicated with the low-temperature cavity (32) is fixedly connected with the upper side wall of the storage tank (28), an elution buffer solution is arranged in the low-temperature cavity (32), a swinging mechanism is connected between the tank body (1) and each sliding plate (3), the swinging mechanism comprises a gear (11) arranged in the tank body (1) and a rotating plate (8) which is rotatably arranged at the lower side of the tank body (1), a fixed plate (8), a fixed shaft (9) is meshed with the outer wall (9) of the motor (3), and the fixed shaft (9) is meshed with the inner wall of the two sliding plates (11), the drive end of motor and centre of a circle fixed connection of left gear (11), digester (15) are including the hollow ware body, the internal slip of hollow ware is provided with two piston boards (18), every all fixedly embedded motor one (19) on piston board (18), two motor one (19) set up relatively, and drive end fixedly connected with crushing dish (20), two the equal fixedly connected with of one end that motor one (19) kept away from mutually runs through connecting rod (16) that the internal wall of hollow ware set up, rotate between connecting rod (16) and rotary board (8) that are located same one side and be connected with bull stick (17), two fixedly connected with telescopic spring (21) between piston board (18) and the inner wall of the hollow ware body.
2. The deep-sea sample nucleic acid in-situ digestion and preservation device according to claim 1, wherein a peristaltic pump (22) is fixed in the tank body (1), and a conveying pipe for conveying a pyrolysis liquid is connected between an output end of the peristaltic pump (22) and the pyrolysis tank (37).
3. The deep-sea sample nucleic acid in-situ digestion and preservation device according to claim 2, characterized in that a plurality of discharge holes (23) are formed in the inner wall of the cracking groove (37), a cavity communicated with the discharge holes (23) is formed in the cracking groove (37), and the output end of the peristaltic pump (22) is communicated with the cavity.
4. The deep-sea sample nucleic acid in-situ digestion and preservation device according to claim 3, wherein the adsorption cavity (31), the drying cavity (33) and the low-temperature cavity (32) are identical in specification, and the connection parts are provided with partition plates for isolation.
5. The deep-sea sample nucleic acid in-situ digestion and preservation device according to claim 4, wherein a rotating shaft (7) is fixedly connected to the end part of the rotating plate (8), a bearing (6) is fixedly sleeved on the end part of the rotating shaft (7), and the bearing (6) is fixed on the inner wall of the tank body (1).
6. The deep-sea sample nucleic acid in-situ digestion and preservation device according to claim 5, wherein a motor II (29) is fixedly embedded in the upper side wall of the preservation tank (28), an electric push rod (36) is vertically and fixedly connected with the driving end of the motor II (29) downwards, a sealing plate I (34) and a sealing plate II (35) are vertically and fixedly connected with the driving end of the electric push rod (36) downwards, and an adsorption column is fixedly connected with the lower side wall of the sealing plate I (34).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410053146.6A CN117568143B (en) | 2024-01-15 | 2024-01-15 | Deep sea sample nucleic acid in-situ digestion and preservation device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202410053146.6A CN117568143B (en) | 2024-01-15 | 2024-01-15 | Deep sea sample nucleic acid in-situ digestion and preservation device |
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| CN117568143A CN117568143A (en) | 2024-02-20 |
| CN117568143B true CN117568143B (en) | 2024-03-22 |
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| CN117842469A (en) * | 2024-03-07 | 2024-04-09 | 诸城兴贸玉米开发有限公司 | Packaging equipment is used in starch sugar production |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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