CN112958019B - Sampling device based on biological experiment equipment and use method thereof - Google Patents

Sampling device based on biological experiment equipment and use method thereof Download PDF

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CN112958019B
CN112958019B CN202011566834.0A CN202011566834A CN112958019B CN 112958019 B CN112958019 B CN 112958019B CN 202011566834 A CN202011566834 A CN 202011566834A CN 112958019 B CN112958019 B CN 112958019B
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sampling
fixedly connected
box
pipe
sliding
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CN112958019A (en
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不公告发明人
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Hangzhou Life Ark Biomedical Technology Co ltd
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Hangzhou Life Ark Biomedical Technology Co ltd
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/18Stationary reactors having moving elements inside
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices
    • G01N2001/1418Depression, aspiration

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hydrology & Water Resources (AREA)
  • Physics & Mathematics (AREA)
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  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention belongs to the technical field of biological experiment equipment, in particular to a sampling device based on biological experiment equipment and a using method thereof, and provides a scheme which comprises a workbench, wherein the top of the workbench is fixedly connected with a biological reaction box, the inner wall of the top and the inner wall of the bottom of the biological reaction box are rotatably connected with the same stirring rod, and the inner wall of the bottom of the workbench is fixedly connected with a stepping motor.

Description

Sampling device based on biological experiment equipment and use method thereof
Technical Field
The invention relates to the technical field of biological experiment equipment, in particular to a sampling device based on biological experiment equipment and a using method thereof.
Background
Bioengineering requires reactions in a bioreactor, meaning any manufacturing or engineering equipment that provides a biologically active environment, in one case a vessel for carrying out chemical processes involving the production of biologically or biochemically active substances from a particular organism, either aerobically or anaerobically, usually cylindrical in volume, ranging from a few liters to several cubic meters, often made of stainless steel.
But prior art's biological reaction unit's sampling device degree of automation is lower, need the manual work to take a sample, increase staff's work load and intensity of labour, and when pulling sample bottle in the sampling process removed, make the bottle empty easily, lead to the bottle to break when serious, the sample flows, need the manual work to wash after using to sampling device in addition, the cleaning efficiency is low, after the washing, the intraductal washing water of imbibition is difficult for clearing up, need natural air-dry, inside remaining liquid if untimely clearance, make impurity such as inside easy absorption dust, influence follow-up use.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a sampling device based on biological experiment equipment, which has a compact structure, reduces the working strength, improves the cleaning efficiency and quickly dries the inner wall of a pipette, and a using method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a sampling device based on biological experiment equipment comprises a workbench, wherein a biological reaction box is fixedly connected to the top of the workbench, the inner wall of the top of the biological reaction box and the inner wall of the bottom of the biological reaction box are rotatably connected with the same stirring rod, a stepping motor is fixedly connected to the inner wall of the bottom of the workbench, an output shaft of the stepping motor extends into the biological reaction box and is fixedly connected with the stirring rod, the top end of the stirring rod extends to the upper part of the biological reaction box and is fixedly connected with an inner toothed ring, a sampling pipe communicated with the top of the biological reaction box is arranged at the top of the biological reaction box, a sampling box is arranged above the biological reaction box, a through hole communicated with the top of the sampling box is arranged at one side of the top of the workbench, a moving component used for enabling the sampling box to move up and down is arranged at one side of the top of the workbench, a rotating shaft is arranged at the bottom of the sampling box, the bottom of the rotating shaft is fixedly connected with a first gear, the inner wall of the bottom of the sampling box is provided with a circular groove, the inner walls of the two sides of the circular groove are connected with a circular sliding plate in a sliding way, the inner wall of the bottom of the circular groove takes the rotating shaft as the circle center, a plurality of tension springs are annularly and equidistantly arranged, the tops of the tension springs are fixedly connected with the bottom of the circular sliding plate, the top of the circular sliding plate is connected with a rotating plate in a sliding way, flourishing appearance bottle has been placed at the top of rotor plate, the top inner wall of flourishing appearance bottle rotates and is connected with the bottle plug, one side inner wall of sampling box is equipped with the drive assembly who is used for removing flourishing appearance bottle, the sampling box drives the top and is equipped with the centre gripping subassembly that is used for the flourishing appearance bottle of centre gripping, top one side of workstation is equipped with the washing subassembly that is used for wasing the biological reaction case.
The movable assembly comprises a support fixedly connected to one side of the top of the workbench, the top inner wall and the bottom inner wall of the support are rotatably connected with the same screw rod, the top end of the screw rod penetrates through the support and is fixedly connected with a first bevel gear, the top of the support is fixedly connected with a rotating motor, an output shaft of the rotating motor is fixedly connected with a second bevel gear, the second bevel gear is meshed with the first bevel gear, the outer wall of the screw rod is in threaded connection with a connecting plate, the connecting plate is in sliding connection with the support, and the inner walls on the two sides of the connecting plate are fixedly connected with a sampling box.
Drive assembly includes the driving motor of fixed connection at sampling case one side inner wall, driving motor's output shaft runs through sampling case and fixedly connected with second synchronizing wheel, the both sides inner wall of sampling case rotates and is connected with two symmetrical screw rods, two the equal threaded connection of outer wall of screw rod has the nut, two the same shifting ring of one side fixedly connected with that the nut is close to each other, flourishing appearance bottle is located the shifting ring, two the one end of screw rod all runs through sampling case and equal fixedly connected with first synchronizing wheel, two the outside transmission of first synchronizing wheel and second synchronizing wheel is connected with same hold-in range.
The clamping component comprises a fixed block fixedly connected to the top of the sampling box, the two inner walls of the two sides of the fixed block are slidably connected with the same sliding block, the two springs and the two springs are fixedly connected to the top of the sampling box, the top of each spring is fixedly connected with the bottom of each sliding block, the two sides of each sliding block are fixedly connected with second racks, the two inner walls of the two sides of each fixed block are rotatably connected with second gears, the two springs are meshed with the second racks, the two sliding rods are fixedly connected with the top of each circular sliding plate, the top of each sliding rod penetrates through the sampling box and is slidably connected with the sampling box, the two sliding rods are fixedly connected with first racks and the two first racks, the first racks are meshed with the second gears, the sampling cylinder and the vacuum tube are fixedly connected to the inside of the sliding block, the bottom of the sampling cylinder and the bottom of the sampling box extend into the sampling box and are slidably connected with the sampling box, the top inner wall of the sampling box is rotatably connected with a rotating ring, the rotating ring is located right above a sample bottle, the top of the vacuum pump is fixedly connected with a vacuum tube, one end of an air inlet of the vacuum pump is connected with a connecting hose, and the connecting tube is connected with the bottom of the sampling box, and the connecting tube is connected with a connecting tube, and the sampling box is connected with the connecting tube.
The cleaning assembly comprises a water suction pump, a water storage tank and a collecting tank which are fixedly connected to the top of a workbench, wherein a first liquid inlet pipe is arranged on a liquid inlet fixing sleeve of the water suction pump, one end of the first liquid inlet pipe extends into the water storage tank, a second liquid inlet pipe which is communicated with the water storage tank is sleeved at the top of the first liquid inlet pipe, a sliding pipe is connected to one side of the sampling box in a sliding mode, one end of the second liquid inlet pipe is communicated with the sliding pipe, one end of the sliding pipe, far away from the second liquid inlet pipe, penetrates through the sliding rod, a first liquid outlet pipe is arranged on a liquid outlet fixing sleeve of the water suction pump, one end of the first liquid outlet pipe extends into the biological reaction box, a second liquid outlet pipe which is communicated with the first liquid outlet pipe is sleeved at one side of the first liquid outlet pipe, one end of the second liquid outlet pipe extends into the collecting tank, and the outer walls of the first liquid inlet pipe, the second liquid inlet pipe, the first liquid outlet pipe and the second liquid outlet pipe are all provided with electromagnetic valves.
Two equal fixedly connected with stopper in one side that the slide bar is close to the nut, the stopper plays limiting displacement to the nut.
The utility model discloses a sampler case, sampling box's one side inner wall fixedly connected with two symmetrical telescopic links, two the one end of telescopic link all with the outer wall fixed connection of shift ring, can play the supporting role to the shift ring through the telescopic link.
States the bottom inner wall of sampling case and is equipped with the recess, one side of sampling case is equipped with the spout, spout and recess are linked together, the both sides inner wall sliding connection of spout has the carriage release lever, the one end that the carriage release lever is close to the sampling case extends to in the recess, the top fixedly connected with circular slab of carriage release lever.
The rubber pad is fixedly connected to the top of the circular plate, and when the sample containing bottle is placed into the sampling box from the through hole, the sample containing bottle can be prevented from being in rigid contact with the inner wall of the bottom of the sampling box through the rubber pad.
The use method of the sampling device based on the biological experimental equipment comprises the following steps:
s1, placing a sample containing bottle in a movable ring from a through hole, starting a driving motor to drive a second synchronous wheel to rotate, driving a first synchronous wheel and a screw to rotate through a synchronous belt by the second synchronous wheel, connecting a nut with the screw in a threaded manner, and driving the movable ring and the sample containing bottle to move rightwards by the nut along with the rotation of the screw so that the sample containing bottle is positioned at the circle center of a rotating plate;
s2, a rotating motor is started to drive a second bevel gear to rotate, the second bevel gear is meshed with a sliding rod, the second bevel gear drives the sliding rod and a lead screw to rotate, the lead screw is in threaded connection with a connecting plate, the connecting plate moves downwards along with the rotation of the lead screw, so that a first gear moves into an inner toothed ring and is meshed with the inner toothed ring, the connecting plate continues to move downwards, a tension spring begins to stretch, a rotating shaft props against a circular sliding plate, the rotating plate and a sample containing bottle to stop moving, the top of the sample containing bottle is contacted with the bottom of the rotating ring, the sample containing bottle is fixed by the rotating ring and the rotating plate, the bottom end of a sampling connecting pipe extends into a sampling pipe, the sliding rod is driven by the circular sliding plate to stop moving, the sampling box drives a fixing block, a second gear and a sliding block to move downwards, and the first rack is meshed with the second gear, so that the first rack drives the second gear to rotate, the second gear is meshed with the second rack, the second rack and the sliding block move downwards along with the rotation of the second gear, a spring begins to compress, and a vacuum tube is driven by the sliding block to move downwards and is penetrated into the sample containing bottle;
s3, the sliding rod is driven by the circular sliding plate to stop moving, the sampling box drives the fixed block, the second gear and the sliding block to move downwards, the first rack is meshed with the second gear, the first rack drives the second gear to rotate, the second gear is meshed with the second rack, the second rack and the sliding block move downwards along with the rotation of the second gear, the spring starts to compress, and the sampling cylinder and the vacuum tube move downwards under the driving of the sliding block to pierce through the bottle plug and enter the sample containing bottle;
s4, starting a vacuum pump, wherein the vacuum pump extracts air in the sample containing bottle through a sampling cylinder and a connecting hose to reduce the pressure in the sample containing bottle, so that the sample in the biological reaction box can be extracted through a vacuum pumping pipe, a sampling connecting pipe and the sampling pipe by the sample containing bottle, starting a stepping motor to drive a stirring rod to rotate, and driving an inner gear ring, a first gear, a rotating plate and the sample containing bottle to rotate by the stirring rod so as to continuously stir the samples in the biological reaction box and the sample containing bottle and prevent the samples in the biological reaction box and the sample containing bottle from agglomerating;
s5, after sampling is finished, starting a driving motor to drive a second synchronous wheel, a first synchronous wheel and a screw rod to rotate, moving the sample containing bottle, the moving ring and the nut to be right below the through hole and right above the circular plate, then moving the moving rod upwards, and driving the circular plate and the sample containing bottle to move upwards by the moving rod, so that the sample containing bottle is conveniently taken out of the sampling box;
s6, during the washing, start the suction pump, open the solenoid valve on first feed liquor pipe and the first drain pipe, the suction pump is gone into the biological reaction incasement through first feed liquor pipe and first drain pipe with the clear water pump in the water storage tank, start step motor and drive the puddler and rotate, stir the clear water in the biological reaction incasement, wash the biological reaction case, then close the solenoid valve on first feed liquor pipe and the first drain pipe, open the solenoid valve on second feed liquor pipe and the second drain pipe, promote the sample connecting pipe to both sides, make the sampling tube can extend to in the sample connecting pipe, start the suction pump after that, the suction pump is gone into the collection incasement through second feed liquor pipe and second drain pipe with the clear water pump in the biological reaction case, and then can be to the sampling tube, the inner wall of sample connecting pipe and sampling tube washs.
S7, starting the circular plate, enabling hot air blown out of the circular plate to enter the biological reaction box to dry the inner wall of the biological reaction box, discharging the hot air from the sampling cylinder along the sampling pipe, the sampling connecting pipe and the sampling cylinder, drying the inner walls of the sampling pipe, the sampling connecting pipe and the sampling cylinder, and preventing residual liquid inside from being cleaned out in time, so that impurities such as dust are easily adsorbed inside, and subsequent use is influenced.
The invention has the following advantages:
1. the sample containing bottle is placed in the movable ring from the through hole, the driving motor is started to drive the second synchronous wheel to rotate, and then the nut, the movable ring and the sample containing bottle can be driven to move rightwards, so that the sample containing bottle is located at the circle center of the rotating plate.
2. The rotating motor is started to drive the second bevel gear to rotate, the connecting plate can be controlled to move downwards along with the rotation of the second bevel gear, the first gear is meshed with the inner gear ring, the rotating ring and the rotating plate can fix the sample containing bottle, and the bottom end of the sampling connecting pipe extends into the sampling pipe.
3. Along with the circular sliding plate drives the sliding rod to stop moving, the sampling box drives the fixed block, the second gear and the sliding block to move downwards, the first rack is meshed with the second gear, the first rack drives the second gear to rotate, the second gear is meshed with the second rack, the second rack and the sliding block move downwards along with the rotation of the second gear, the spring starts to compress, and the sampling cylinder and the vacuum tube move downwards under the driving of the sliding block to pierce through the bottle plug to enter the sample containing bottle.
4. The vacuum pump is started, the vacuum pump extracts the air in the sample containing bottle through the sampling tube and the connecting hose, reduce the pressure in the sample containing bottle, and then the sample containing bottle can be through the evacuation pipe, sample connecting pipe and sampling tube extract the sample in the biological reaction box, start step motor drive puddler and rotate, the pinion drives interior ring gear, first gear, rotor plate and sample containing bottle and rotate, and then can continuously stir the sample in biological reaction box and the sample containing bottle, prevent the sample reunion in biological reaction box and the sample containing bottle.
5. After the sample is ended, start driving motor drive second synchronizing wheel, first synchronizing wheel and screw rod and rotate, will hold appearance bottle, shift ring and nut and move to the through-hole under, directly over the circular slab, then the rebound carriage release lever, the carriage release lever drives circular slab and flourishing appearance bottle rebound, conveniently will hold the appearance bottle and take out from the sample chamber.
6. When the cleaning machine is used for cleaning, the suction pump is started, the electromagnetic valves on the first liquid inlet pipe and the first liquid outlet pipe are opened, the suction pump pumps clean water in the water storage tank into a biological reaction box through the first liquid inlet pipe and the first liquid outlet pipe, the step motor is started to drive the stirring rod to rotate, clean water in the biological reaction box is stirred, the biological reaction box is cleaned, the electromagnetic valves on the first liquid inlet pipe and the first liquid outlet pipe are closed, the electromagnetic valves on the second liquid inlet pipe and the second liquid outlet pipe are opened, the sampling connecting pipe is pushed to two sides, the sampling cylinder can be extended into the sampling connecting pipe, then the suction pump is started, the suction pump pumps clean water in the biological reaction box into the collection box through the second liquid inlet pipe and the second liquid outlet pipe, and then the sampling pipe and the inner wall of the sampling connecting pipe and the sampling cylinder can be cleaned.
7. After finishing when wasing, start the circular slab, the hot-blast inner wall that gets into the biological reaction case that the circular slab blew off is dried the biological reaction case, and hot-blast along with sampling tube, sample connecting pipe and sampler barrel discharge from the sampling barrel, can dry the inner wall of sampling tube, sample connecting pipe and sampler barrel, prevents that inside remaining liquid is untimely to be cleared up, makes impurity such as inside easy absorption dust, influences follow-up use.
The sample taking device is simple in structure and convenient to operate, the movable ring can be driven by the driving motor to move, the bottle body is prevented from being toppled to cause bottle body breakage in the moving process of the sample taking bottle, air in the sample taking bottle can be pumped by the vacuum pump to form negative pressure so that a sample can be pumped, and in addition, the cleaning and drying are integrated, so that the cleaning efficiency is improved, and the phenomenon that residual liquid exists on the inner walls of the biological reaction box, the sampling pipe, the sampling connecting pipe and the sampling cylinder to influence subsequent use can be prevented.
Description of the figures and
FIG. 1 is a front cross-sectional view of a sampling device based on biological experimental equipment according to the present invention;
FIG. 2 is a side view of a sampling device based on a biological experimental facility;
FIG. 3 is a front cross-sectional view of a connection plate of a sampling device based on biological experimental equipment according to the present invention;
FIG. 4 is a top cross-sectional view of a frame of a sampling device based on biological experimental equipment according to the present invention;
FIG. 5 is a side sectional view of a sampling box of a sampling device based on biological experimental equipment according to the present invention;
FIG. 6 is a top cross-sectional view of a sampling box of a sampling device based on biological experimental equipment according to the present invention;
FIG. 7 is a top view of a sampling box of a sampling device based on biological experimental equipment according to the present invention;
FIG. 8 is a top view of an inner gear ring of a sampling device based on biological experimental equipment according to the present invention;
FIG. 9 is a front cross-sectional view of a sample holding bottle of a sampling device based on biological experimental equipment, which is provided by the invention;
FIG. 10 is a side view of a mobile ring of a sampling device based on biological experimental equipment according to the present invention;
FIG. 11 is a top view of a rotating plate of a sampling device based on biological experimental equipment according to the present invention;
FIG. 12 is an enlarged view of a sampling device based on a biological experimental facility;
FIG. 13 is an enlarged view of a sampling device based on biological experimental equipment, shown at B;
FIG. 14 is a top sectional view of the sampling box according to the second embodiment;
FIG. 15 is a partial front sectional view of a sampling box according to the second embodiment.
In the figure: 1. a work table; 2. a biological reaction box; 3. a sampling tube; 4. a stirring rod; 5. an inner gear ring; 6. a support; 7. a lead screw; 8. a connecting plate; 9. a first bevel gear; 10. rotating the motor; 11. a second bevel gear; 12. a sampling box; 13. a circular groove; 14. a circular sliding plate; 15. a tension spring; 16. a rotating plate; 17. a rotating shaft; 18. a first gear; 19. a slide bar; 20. a sample containing bottle; 21. a bottle stopper; 22. a screw; 23. a nut; 24. a moving ring; 25. a first synchronizing wheel; 26. a drive motor; 27. a second synchronizing wheel; 28. a synchronous belt; 29. rotating the circular ring; 30. a fixed block; 31. a slider; 32. a second gear; 33. a first rack; 34. a second rack; 35. a spring; 36. a sampling tube; 37. vacuumizing a tube; 38. a vacuum pump; 39. a connecting hose; 40. a sampling connecting pipe; 41. a water pump; 42. a first liquid inlet pipe; 43. a water storage tank; 44. a second liquid inlet pipe; 45. a through hole; 46. a first liquid outlet pipe; 47. a second liquid outlet pipe; 48. a collection box; 49. an electromagnetic valve; 50. a circular plate; 51. a chute; 52. a travel bar; 53. a groove; 54. a rubber pad; 55. a limiting block; 56. a telescopic rod; 57. a stepping motor; 58. a sliding tube.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example one
Referring to fig. 1-13, a sampling device based on biological experimental equipment, including a workbench 1, a biological reaction box 2 is fixedly connected to the top of the workbench 1, the top inner wall and the bottom inner wall of the biological reaction box 2 are rotatably connected with the same stirring rod 4, a stepping motor 57 is fixedly connected to the bottom inner wall of the workbench 1, an output shaft of the stepping motor 57 extends into the biological reaction box 2 and is fixedly connected with the stirring rod 4, the top end of the stirring rod 4 extends to the upper side of the biological reaction box 2 and is fixedly connected with an inner toothed ring 5, a sampling tube 3 is connected to the top of the biological reaction box 2, a sampling box 12 is arranged above the biological reaction box 2, through holes 45 communicated with the top of the sampling box 12 are provided, a moving assembly for moving the sampling box 12 up and down is provided on one side of the top of the workbench 1, a rotating shaft 17 is provided on the bottom of the sampling box 12, a first gear 18 is fixedly connected to the bottom inner wall of the rotating shaft 17, a circular groove 13 is provided on the bottom inner wall of the sampling box 12, circular sliding plates 14 are slidably connected with circular sliding plates 14, a plurality of inner walls of the rotating bottle holding assembly 20, a plurality of the rotating bottle plug 15 arranged on the inner wall of the rotating box 12, a plurality of the rotating plate 16 is provided on one side of the rotating bottle holding assembly for holding the bottle holding and is provided with a bottle holding assembly for holding plug 20 for holding the bottle cleaning, a plurality of the bottle 20, a plurality of the rotating bottle holding assembly for holding plug 20, a bottle holding plug for holding plug 20.
According to the invention, the moving assembly comprises a support 6 fixedly connected to one side of the top of the workbench 1, the top inner wall and the bottom inner wall of the support 6 are rotatably connected with a same screw 7, the top end of the screw 7 penetrates through the support 6 and is fixedly connected with a first bevel gear 9, the top of the support 6 is fixedly connected with a rotating motor 10, an output shaft of the rotating motor 10 is fixedly connected with a second bevel gear 11, the second bevel gear 11 is meshed with the first bevel gear 9, the outer wall of the screw 7 is in threaded connection with a connecting plate 8, the connecting plate 8 is in sliding connection with the support 6, and the inner walls on two sides of the connecting plate 8 are fixedly connected with a sampling box 12.
In the invention, the driving assembly comprises a driving motor 26 fixedly connected to the inner wall of one side of the sampling box 12, the output shaft of the driving motor 26 penetrates through the sampling box 12 and is fixedly connected with a second synchronizing wheel 27, the inner walls of two sides of the sampling box 12 are rotatably connected with two symmetrical screw rods 22, the outer walls of the two screw rods 22 are both in threaded connection with nuts 23, one side of each adjacent nut 23 is fixedly connected with a same moving ring 24, the sample containing bottle 20 is positioned in the moving ring 24, one ends of the two screw rods 22 penetrate through the sampling box 12 and are both fixedly connected with first synchronizing wheels 25, and the outer sides of the two first synchronizing wheels 25 and the second synchronizing wheel 27 are in transmission connection with a same synchronous belt 28.
In the invention, the clamping assembly comprises a fixed block 30 fixedly connected to the top of the sampling box 12, the inner walls of two sides of the fixed block 30 are slidably connected with a same sliding block 31, the top of the sampling box 12 is fixedly connected with two springs 35, the top ends of the two springs 35 are fixedly connected with the bottom of the sliding block 31, two sides of the sliding block 31 are fixedly connected with second racks 34, the inner walls of two sides of the fixed block 30 are rotatably connected with second gears 32, the side, close to each other, of the two second gears 32 is meshed with the second racks 34, two sides of the top of the circular sliding plate 14 are fixedly connected with sliding rods 19, the tops of the two sliding rods 19 are respectively penetrated through the sampling box 12 and are slidably connected with the sampling box 12, one side, close to each other, of the two first racks 33 is fixedly connected with the second gears 32, a sampling cylinder 36 and a vacuum tube 37 are fixedly connected in the sliding block 31, the bottom ends of the sampling cylinder 36 and the vacuum tube 37 extend into the sampling box 12 and are slidably connected with the sampling box 12, the inner wall of the top of the sampling box 12 is rotatably connected with a rotating ring 29, the vacuum tube 29 is positioned above the sampling bottle 20, a connecting tube 38 is connected with a vacuum tube 40, and a connecting tube 38 connected with a connecting tube 40, and a connecting tube 40 connected with the top of the sampling box 12.
According to the invention, the cleaning assembly comprises a water suction pump 41, a water storage tank 43 and a collection tank 48 which are fixedly connected to the top of the workbench 1, a first liquid inlet pipe 42 is fixedly sleeved on a liquid inlet of the water suction pump 41, one end of the first liquid inlet pipe 42 extends into the water storage tank 43, a second liquid inlet pipe 44 communicated with the first liquid inlet pipe 42 is fixedly sleeved on the top of the first liquid inlet pipe 42, a sliding pipe 58 is slidably connected to one side of the sampling tank 12, one end of the second liquid inlet pipe 44 is communicated with the sliding pipe 58, one end, far away from the second liquid inlet pipe 44, of the sliding pipe 58 penetrates through the sliding rod 19, a first liquid outlet pipe 46 is fixedly sleeved on a liquid outlet of the water suction pump 41, one end of the first liquid outlet pipe 46 extends into the biological reaction tank 2, a second liquid outlet pipe 47 communicated with the first liquid outlet pipe 46 is fixedly sleeved on one side of the first liquid outlet pipe 46, one end of the second liquid outlet pipe 47 extends into the collection tank 48, and electromagnetic valves 49 are fixedly sleeved on outer walls of the first liquid inlet pipe 42, the second liquid outlet pipe 44, the first liquid outlet pipe 46 and the second liquid outlet pipe 47.
In the invention, the two sliding rods 19 are fixedly connected with the limiting blocks 55 at one sides close to the nuts 23, and the limiting blocks 55 limit the nuts 23.
In the invention, two symmetrical telescopic rods 56 are fixedly connected to the inner wall of one side of the sampling box 12, one ends of the two telescopic rods 56 are both fixedly connected with the outer wall of the moving ring 24, and the moving ring 24 can be supported by the telescopic rods 56.
In the invention, a groove 53 is arranged on the inner wall of the bottom of the sampling box 12, a sliding chute 51 is arranged on one side of the sampling box 12, the sliding chute 51 is communicated with the groove 53, the inner walls of the two sides of the sliding chute 51 are connected with a movable rod 52 in a sliding manner, one end of the movable rod 52 close to the sampling box 12 extends into the groove 53, and the top of the movable rod 52 is fixedly connected with a circular plate 50.
A method for using a sampling device based on biological experimental equipment comprises the following steps:
s1, placing a sample containing bottle 20 in a moving ring 24 from a through hole 45, starting a driving motor 26 to drive a second synchronous wheel 27 to rotate, driving a first synchronous wheel 25 and a screw rod 22 to rotate by the second synchronous wheel 27 through a synchronous belt 28, connecting a nut 23 with the screw rod 22 in a threaded manner, and driving the moving ring 24 and the sample containing bottle 20 to move rightwards by the nut 23 along with the rotation of the screw rod 22 so that the sample containing bottle 20 is positioned at the circle center position of a rotating plate 16;
s2, starting the rotating motor 10 to drive the second bevel gear 11 to rotate, the second bevel gear 11 and the sliding rod 19 are meshed, the second bevel gear 11 drives the sliding rod 19 and the lead screw 7 to rotate, the lead screw 7 and the connecting plate 8 are in threaded connection, the connecting plate 8 moves downwards along with the rotation of the lead screw 7, the first gear 18 moves into the inner toothed ring 5 and is meshed with the inner toothed ring 5, the tension spring 15 starts to stretch along with the downward movement of the connecting plate 8, the rotating shaft 17 abuts against the circular sliding plate 14, the rotating plate 16 and the sample containing bottle 20 to stop moving, the top of the sample containing bottle 20 is contacted with the bottom of the rotating ring 29, the rotating ring 29 and the rotating plate 16 fix the sample containing bottle 20, the bottom end of the sampling connecting pipe 40 extends into the sampling pipe 3, the sampling box 12 drives the fixed block 30, the second gear 32 and the sliding block 31 to move downwards along with the sliding rod 19 driven by the circular sliding plate 14, the first rack 33 is meshed with the second rack 32, the second rack 32 is meshed with the second rack 34 and the sliding block 31 moves downwards along with the sliding block 35, and the sliding block 35 and compresses the sliding block 31 moving along with the sampling pipe 32;
s3, as the circular sliding plate 14 drives the sliding rod 19 to stop moving, the sampling box 12 drives the fixed block 30, the second gear 32 and the sliding block 31 to move downwards, and the first rack 33 is meshed with the second gear 32, so that the first rack 33 drives the second gear 32 to rotate, the second gear 32 is meshed with the second rack 34, the second rack 34 and the sliding block 31 move downwards along with the rotation of the second gear 32, the spring 35 starts to compress, and the sampling cylinder 36 and the vacuum tube 37 move downwards under the driving of the sliding block 31 to pierce through the bottle stopper 21 and enter the sample containing bottle 20;
s4, starting a vacuum pump 38, wherein the vacuum pump 38 extracts air in the sample containing bottle 20 through a sampling cylinder 36 and a connecting hose 39 to reduce the pressure intensity in the sample containing bottle 20, so that the sample containing bottle 20 can extract samples in the biological reaction box 2 through a vacuum tube 37, a sampling connecting tube 40 and a sampling tube 3, starting a stepping motor 57 to drive a stirring rod 4 to rotate, and the stirring rod 4 drives an inner gear ring 5, a first gear 18, a rotating plate 16 and the sample containing bottle 20 to rotate, so that the samples in the biological reaction box 2 and the sample containing bottle 20 can be continuously stirred, and the samples in the biological reaction box 2 and the sample containing bottle 20 are prevented from being agglomerated;
s5, after sampling is finished, starting the driving motor 26 to drive the second synchronous wheel 27, the first synchronous wheel 25 and the screw 22 to rotate, moving the sample containing bottle 20, the moving ring 24 and the nut 23 to be right below the through hole 45 and right above the circular plate 50, then moving the moving rod 52 upwards, and driving the circular plate 50 and the sample containing bottle 20 to move upwards by the moving rod 52, so that the sample containing bottle 20 can be conveniently taken out of the sampling box 12;
s6, during cleaning, a water suction pump 41 is started, electromagnetic valves 49 on a first liquid inlet pipe 42 and a first liquid outlet pipe 46 are opened, the water suction pump 41 pumps clean water in a water storage tank 43 into the biological reaction box 2 through the first liquid inlet pipe 42 and the first liquid outlet pipe 46, a stepping motor 57 is started to drive a stirring rod 4 to rotate, the clean water in the biological reaction box 2 is stirred to clean the biological reaction box 2, then the electromagnetic valves 49 on the first liquid inlet pipe 42 and the first liquid outlet pipe 46 are closed, the electromagnetic valves 49 on a second liquid inlet pipe 44 and a second liquid outlet pipe 47 are opened to push a sampling connecting pipe 40 to two sides, so that a sampling cylinder 36 can extend into the sampling connecting pipe 40, then the water suction pump 41 is started, the water suction pump 41 pumps the clean water in the biological reaction box 2 into a collecting box 48 through the second liquid inlet pipe 44 and the second liquid outlet pipe 47, and further the inner walls of the sampling pipe 3, the sampling connecting pipe 40 and the sampling cylinder 36 can be cleaned;
s7, starting the circular plate 50, enabling hot air blown out from the circular plate 50 to enter the biological reaction box 2 to dry the inner wall of the biological reaction box 2, discharging the hot air from the sampling cylinder 36 along the sampling tube 3, the sampling connecting tube 40 and the sampling cylinder 36, drying the inner walls of the sampling tube 3, the sampling connecting tube 40 and the sampling cylinder 36, preventing the residual liquid inside from being not cleaned in time, enabling the interior to easily absorb impurities such as dust and the like, and influencing subsequent use.
Example two: as shown in fig. 13-15, a sampling device based on biological experimental equipment, the present embodiment is different from the first embodiment in that: the rubber pad 54 is fixedly connected to the top of the circular plate 50, and when the sample bottle 20 is placed into the sampling box 12 from the through hole 45, the rubber pad 54 can prevent the sample bottle 20 from being in hard contact with the inner wall of the bottom of the sampling box 12.
However, as is well known to those skilled in the art, the working principle and wiring method of the rotating motor 10, the driving motor 26, the vacuum pump 38, the suction pump 41, the solenoid valve 49, the circular plate 50, the telescopic rod 56 and the stepping motor 57 are common and are conventional means or common knowledge, and thus they will not be described herein again, and those skilled in the art can make any choice according to their needs or convenience.
The working principle is as follows: firstly, placing a sample containing bottle 20 in a moving ring 24 from a through hole 45, starting a driving motor 26 to drive a second synchronizing wheel 27 to rotate, enabling the second synchronizing wheel 27 to drive a first synchronizing wheel 25 and a screw 22 to rotate through a synchronous belt 28, enabling a nut 23 to be in threaded connection with the screw 22, enabling the nut 23 to drive the moving ring 24 and the sample containing bottle 20 to move rightwards along with the rotation of the screw 22, enabling the sample containing bottle 20 to be located at the center position of a rotating plate 16, secondly, starting a rotating motor 10 to drive a second bevel gear 11 to rotate, enabling the second bevel gear 11 to be meshed with a sliding rod 19, enabling the sliding rod 19 and a lead screw 7 to rotate along with the rotation of the screw 22, enabling the lead screw 7 to move downwards along with the rotation of the connecting plate 8, enabling a first gear 18 to move into an inner toothed ring 5 and be meshed with the inner toothed ring 5, as the connecting plate 8 continues to move downwards, a tension spring 15 starts to stretch, a rotating shaft 17 props against a circular sliding plate 14, the rotating plate 16 and the sampling plate 20 to stop moving, enabling the top of the sample containing bottle 20 and the rotating ring 29 to touch the rotating ring 29, the rotating ring 29 and the rotating ring 16 to move downwards along with the rotating ring 20, enabling a vacuum pump 20 to drive a sliding block 32 to move towards a vacuum pump 20, enabling a sliding block 32 to drive a sliding block 32 along with the sliding block 32, enabling a vacuum pump 20 to move towards the sliding block 32, enabling a vacuum pump 20 to move, enabling a first bevel gear 32 to move towards the sliding block 32, enabling a vacuum bottle 32 and a vacuum bottle plug 32, enabling a vacuum pump 20 to move towards the sliding block 32, enabling a vacuum bottle plug 32 to move along with the sliding block 32 to move along with a first bevel gear 32, enabling a vacuum pump 20 and a vacuum bottle plug 32, enabling a first bevel gear 32, the vacuum pump 38 extracts air in the sample bottle 20 through the sampling tube 36 and the connecting hose 39 to reduce the pressure in the sample bottle 20, and then the sample bottle 20 can extract samples in the biological reaction box 2 through the vacuum tube 37, the sampling connecting tube 40 and the sampling tube 3, the stepping motor 57 is started to drive the stirring rod 4 to rotate, the stirring rod 4 drives the inner toothed ring 5, the first gear 18, the rotating plate 16 and the sample bottle 20 to rotate, so that the samples in the biological reaction box 2 and the sample bottle 20 can be continuously stirred to prevent the samples in the biological reaction box 2 and the sample bottle 20 from agglomerating, the fourth step is started, after sampling is finished, the driving motor 26 is started to drive the second synchronizing wheel 27, the first synchronizing wheel 25 and the screw rod 22 to rotate, the sample bottle 20, the moving ring 24 and the nut 23 are moved to be right below the through hole 45, to be right above the circular plate 50, and then the moving rod 52 is moved upwards, the moving rod 52 drives the circular plate 50 and the sample containing bottle 20 to move upwards, so that the sample containing bottle 20 can be conveniently taken out of the sample taking box 12, in the fifth step, during cleaning, the water suction pump 41 is started, the electromagnetic valves 49 on the first liquid inlet pipe 42 and the first liquid outlet pipe 46 are opened, the water suction pump 41 pumps clean water in the water storage tank 43 into the biological reaction box 2 through the first liquid inlet pipe 42 and the first liquid outlet pipe 46, the stepping motor 57 is started to drive the stirring rod 4 to rotate, clean water in the biological reaction box 2 is stirred to clean the biological reaction box 2, then the electromagnetic valves 49 on the first liquid inlet pipe 42 and the first liquid outlet pipe 46 are closed, the electromagnetic valves 49 on the second liquid inlet pipe 44 and the second liquid outlet pipe 47 are opened, the sampling connecting pipe 40 is pushed towards two sides, so that the sampling cylinder 36 can extend into the sampling connecting pipe 40, then the water suction pump 41 is started, and the water suction pump 41 pumps clean water in the biological reaction box 2 into the collection box 48 through the second liquid inlet pipe 44 and the second liquid outlet pipe 47, and then can wash the inner wall of sampling tube 3, sample connecting pipe 40 and sampler barrel 36, the sixth step, after finishing washing, start circular plate 50, the hot-blast entering that circular plate 50 blew off dries the inner wall of biological reaction case 2 in the biological reaction case 2, hot-blast following sampling tube 3, sample connecting pipe 40 and sampler barrel 36 and discharging, can dry the inner wall of sampling tube 3, sample connecting pipe 40 and sampler barrel 36, prevent that inside remaining liquid from not in time clearing up, make inside easily adsorb impurity such as dust, influence follow-up use.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.

Claims (8)

1. The utility model provides a sampling device based on biological assay equipment, includes workstation (1), its characterized in that, the top fixedly connected with biological reaction case (2) of workstation (1), the top inner wall and the bottom inner wall of biological reaction case (2) rotate and be connected with same puddler (4), the bottom inner wall fixedly connected with step motor (57) of workstation (1), the output shaft of step motor (57) extend to in biological reaction case (2) and with puddler (4) fixed connection, the top of puddler (4) extends to the top of biological reaction case (2) and fixedly connected with inner gear ring (5), the top of biological reaction case (2) is equipped with sampling tube (3) that are linked together, the top of biological reaction case (2) is equipped with sampling box (12), the top of sampling box (12) is equipped with through-hole (45) that are linked together, top one side of workstation (1) is equipped with the removal subassembly that is used for making sampling box (12) reciprocate, the bottom of sampling box (12) is equipped with pivot (17), the bottom of pivot (17) fixed gear wheel bottom be connected with first circular shape gear (18), the circular shape groove (13) inner wall (13) of sliding plate (13) is connected with circular shape groove (13) bottom sliding groove (13) that the circular shape groove (13) of circle of having been connected with the circular shape groove (13) and has been arranged, circular shape groove (13) both sides, circular shape inner wall (13) and be connected with the bottom of circular shape groove (13) inner wall (13) are equipped with the bottom of circular shape, circular shape groove (13) inner wall (13) are equipped with the bottom of circular shape The device comprises a plurality of tension springs (15), the tops of the tension springs (15) are fixedly connected with the bottom of a circular sliding plate (14), the top of the circular sliding plate (14) is connected with a rotating plate (16) in a sliding mode, sample containing bottles (20) are placed on the top of the rotating plate (16), the inner walls of the tops of the sample containing bottles (20) are connected with bottle stoppers (21) in a rotating mode, a driving assembly used for moving the sample containing bottles (20) is arranged on the inner wall of one side of a sampling box (12), a clamping assembly used for clamping the sample containing bottles (20) is arranged on the top of the sampling box (12), a cleaning assembly used for cleaning a biological reaction box (2) is arranged on one side of the top of a workbench (1), and the biological reaction box (2) is communicated with a drying assembly;
the moving assembly comprises a support (6) fixedly connected to one side of the top of the workbench (1), the inner wall of the top and the inner wall of the bottom of the support (6) are rotatably connected with a same lead screw (7), the top end of the lead screw (7) penetrates through the support (6) and is fixedly connected with a first bevel gear (9), the top of the support (6) is fixedly connected with a rotating motor (10), an output shaft of the rotating motor (10) is fixedly connected with a second bevel gear (11), the second bevel gear (11) is meshed with the first bevel gear (9), the outer wall of the lead screw (7) is in threaded connection with a connecting plate (8), the connecting plate (8) is in sliding connection with the support (6), and the connecting plate (8) is fixedly connected with a sampling box (12);
the clamping assembly comprises a fixed block (30) fixedly connected to the top of the sampling box (12), a sliding block (31) is arranged in an inner cavity of the fixed block (30), two springs (35) are fixedly connected to the top of the sampling box (12), the top ends of the two springs (35) are fixedly connected to the bottom of the sliding block (31), second racks (34) are fixedly connected to two sides of the sliding block (31), second gears (32) are rotatably connected to inner walls of two sides of the fixed block (30), one sides, close to each other, of the two second gears (32) are respectively meshed with the second racks (34), sliding rods (19) are fixedly connected to two sides of the top of the circular sliding plate (14), the tops of the two sliding rods (19) respectively penetrate through the sampling box (12) and are respectively slidably connected with the sampling box (12), one sides, close to each other, of the two sliding rods (19) are respectively fixedly connected with a first rack (33), the two first racks (33) are respectively meshed with the second gears (32), a vacuum tube (36) and a vacuum tube (37) are fixedly connected to the inner wall of the sampling box (31), and a rotating ring (29) is connected with the inner wall of the sampling box (12), rotate ring (29) and be located flourishing appearance bottle (20) directly over, the top fixedly connected with vacuum pump (38) of sampling case (12), the fixed cover of air inlet of vacuum pump (38) is equipped with coupling hose (39), the one end and the evacuation pipe (37) of coupling hose (39) are linked together, the bottom fixedly connected with sample connecting pipe (40) of sampling case (12), sample connecting pipe (40) are located sampling tube (3) directly over, the top of sample connecting pipe (40) is run through sampling case (12) and is linked together with sampling tube (36).
2. The sampling device based on biological assay equipment of claim 1, characterized in that, drive assembly includes drive motor (26) of fixed connection at sampling case (12) one side inner wall, the output shaft of drive motor (26) runs through sampling case (12) and fixedly connected with second synchronizing wheel (27), the both sides inner wall of sampling case (12) is rotated and is connected with two symmetrical screw rods (22), two the equal threaded connection of outer wall of screw rod (22) has nut (23), two one side fixedly connected with same shift ring (24) that nut (23) are close to each other, flourishing appearance bottle (20) is located shift ring (24), two the one end of screw rod (22) all runs through sampling case (12) and equal fixedly connected with first synchronizing wheel (25), two the outside transmission of first synchronizing wheel (25) and second synchronizing wheel (27) is connected with same hold-in range (28).
3. The biological experiment apparatus-based sampling device of claim 1, the cleaning component comprises a water suction pump (41), a water storage tank (43) and a collecting box (48) which are fixedly connected with the top of the workbench (1), a first liquid inlet pipe (42) is fixedly sleeved on a liquid inlet of the water pump (41), one end of the first liquid inlet pipe (42) extends into the water storage tank (43), the top of the first liquid inlet pipe (42) is fixedly sleeved with a second liquid inlet pipe (44) which is communicated with the first liquid inlet pipe, one side of the sampling box (12) is connected with a sliding pipe (58) in a sliding way, one end of the second liquid inlet pipe (44) is communicated with the sliding pipe (58), one end of the sliding pipe (58) far away from the second liquid inlet pipe (44) penetrates through the sliding rod (19), a first liquid outlet pipe (46) is fixedly sleeved at a liquid outlet of the water pump (41), one end of the first liquid outlet pipe (46) extends into the biological reaction box (2), one side of the first liquid outlet pipe (46) is fixedly sleeved with a second liquid outlet pipe (47) communicated with the first liquid outlet pipe, one end of the second liquid outlet pipe (47) extends into the collecting box (48), the outer walls of the first liquid inlet pipe (42), the second liquid inlet pipe (44), the first liquid outlet pipe (46) and the second liquid outlet pipe (47) are fixedly sleeved with electromagnetic valves (49).
4. The sampling device based on the biological experiment equipment as claimed in claim 1, wherein one side of each of the two sliding rods (19) close to the nut (23) is fixedly connected with a limiting block (55), and the limiting blocks (55) have a limiting effect on the nut (23).
5. The sampling device based on biological experiment equipment according to claim 2, characterized in that two symmetrical telescopic rods (56) are fixedly connected to the inner wall of one side of the sampling box (12), and one end of each of the two telescopic rods (56) is fixedly connected with the outer wall of the movable ring (24).
6. The sampling device based on biological experiment equipment according to claim 1, characterized in that the bottom inner wall of the sampling box (12) is provided with a groove (53), one side of the sampling box (12) is provided with a sliding groove (51), the sliding groove (51) is communicated with the groove (53), the inner walls of the two sides of the sliding groove (51) are slidably connected with a movable rod (52), one end of the movable rod (52) close to the sampling box (12) extends into the groove (53), and the top of the movable rod (52) is fixedly connected with a circular plate (50).
7. The biological experiment equipment-based sampling device according to claim 6, wherein the rubber pad (54) is fixedly connected to the top of the circular plate (50), and when the sample-holding bottle (20) is put into the sampling box (12) from the through hole (45), the sample-holding bottle (20) is prevented from being in hard contact with the inner wall of the bottom of the sampling box (12) by the rubber pad (54).
8. The method of claim 7, wherein the sampling device comprises: the method comprises the following steps:
s1, placing a sample containing bottle (20) in a moving ring (24) from a through hole (45), starting a driving motor (26) to drive a second synchronous wheel (27) to rotate, driving a first synchronous wheel (25) and a screw rod (22) to rotate by the second synchronous wheel (27) through a synchronous belt (28), connecting a nut (23) with the screw rod (22) in a threaded manner, and driving the moving ring (24) and the sample containing bottle (20) to move rightwards by the nut (23) along with the rotation of the screw rod (22) so that the sample containing bottle (20) is located at the circle center position of a rotating plate (16);
s2, a rotating motor (10) is started to drive a second bevel gear (11) to rotate, the second bevel gear (11) is meshed with a first bevel gear (9), the second bevel gear (11) drives the first bevel gear (9) and a lead screw (7) to rotate, the lead screw (7) is in threaded connection with a connecting plate (8), the connecting plate (8) moves downwards along with the rotation of the lead screw (7), so that a first gear (18) moves into an inner gear ring (5) and is meshed with the inner gear ring (5), a tension spring (15) begins to stretch along with the continuous downward movement of the connecting plate (8), a rotating shaft (17) props against a rotating plate (16) and stops moving with a sample containing bottle (20), the top of the sample containing bottle (20) is contacted with the bottom of the rotating ring (29), the rotating ring (29) and the rotating plate (16) fix the sample containing bottle (20), and the bottom of a sampling connecting pipe (40) extends into a sampling pipe (3);
s3, the sliding rod (19) is driven to stop moving along with the circular sliding plate (14), the sampling box (12) drives the fixed block (30), the second gear (32) and the sliding block (31) to move downwards, and the first rack (33) is meshed with the second gear (32), so that the first rack (33) drives the second gear (32) to rotate, the second gear (32) is meshed with the second rack (34), the second rack (34) and the sliding block (31) move downwards along with the rotation of the second gear (32), the spring (35) starts to compress, and the sampling cylinder (36) and the vacuum tube (37) move downwards under the driving of the sliding block (31) to pierce through the bottle stopper (21) and enter the sample containing bottle (20);
s4, starting a vacuum pump (38), extracting air in the sample containing bottle (20) through a sampling cylinder (36) and a connecting hose (39) by the vacuum pump (38), reducing the pressure in the sample containing bottle (20), further extracting samples in the biological reaction box (2) through a vacuum-extracting pipe (37), a sampling connecting pipe (40) and a sampling pipe (3) by the sample containing bottle (20), starting a stepping motor (57) to drive a stirring rod (4) to rotate, driving an inner toothed ring (5), a first gear (18), a rotating plate (16) and the sample containing bottle (20) to rotate by the stirring rod (4), further continuously stirring or uniformly mixing the samples in the biological reaction box (2) and the sample containing bottle (20), and preventing the samples in the biological reaction box (2) and the sample containing bottle (20) from aggregating;
s5, after sampling is finished, starting a driving motor (26) to drive a second synchronous wheel (27), a first synchronous wheel (25) and a screw (22) to rotate, moving the sample containing bottle (20), a moving ring (24) and a nut (23) to be right below a through hole (45) and right above a circular plate (50), then moving a moving rod (52) upwards, and driving the circular plate (50) and the sample containing bottle (20) to move upwards by the moving rod (52), so that the sample containing bottle (20) can be conveniently taken out of a sampling box (12);
s6, during cleaning, a water suction pump (41) is started, electromagnetic valves (49) on a first liquid inlet pipe (42) and a first liquid outlet pipe (46) are opened, the water suction pump (41) pumps clean water in a water storage tank (43) into a biological reaction box (2) through the first liquid inlet pipe (42) and the first liquid outlet pipe (46), a stepping motor (57) is started to drive a stirring rod (4) to rotate, clean water in the biological reaction box (2) is stirred to clean the biological reaction box (2), then the electromagnetic valves (49) on the first liquid inlet pipe (42) and the first liquid outlet pipe (46) are closed, the electromagnetic valves (49) on a second liquid inlet pipe (44) and a second liquid outlet pipe (47) are opened, a sampling connecting pipe (40) is pushed towards two sides, a sampling cylinder (36) extends into the sampling connecting pipe (40), then the water suction pump (41) is started, the water suction pump (41) pumps clean water in the biological reaction box (2) into the sampling connecting pipe (48) through the second liquid inlet pipe (44) and the second liquid outlet pipe (47), and the inner wall of the sampling cylinder (3) is cleaned;
s7, starting a drying component, enabling hot air blown out by the drying component to enter the biological reaction box (2) to dry the inner wall of the biological reaction box (2), discharging the hot air from the sampling cylinder (36) along the sampling pipe (3), the sampling connecting pipe (40) and the sampling cylinder (36), and drying the inner walls of the sampling pipe (3), the sampling connecting pipe (40) and the sampling cylinder (36).
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