CN212051399U

CN212051399U - Novel single sperm microinjection ware

Info

Publication number: CN212051399U
Application number: CN202020307034.6U
Authority: CN
Inventors: 马超; 李利娜; 邵小光
Original assignee: Dalian Municipal Women And Children's Medical Center
Current assignee: Dalian Municipal Women And Children's Medical Center
Priority date: 2020-03-13
Filing date: 2020-03-13
Publication date: 2020-12-01
Anticipated expiration: 2030-03-13

Abstract

The invention discloses a novel single sperm microinjection dish, which is applied to the field of single sperm microinjection and comprises a base, wherein the upper surface of the base comprises a horizontal long-strip plane, one side of the long-strip plane along the width direction is an inclined plane with the bottom end connected with the long-strip plane, and the other side of the long-strip plane along the width direction is a vertical step higher than the long-strip plane; a plurality of cylindrical concave holes are formed in the inclined plane; the diameter and the column height of the concave hole of the cylinder are both 200-250 um; the width of the long strip plane is 200 um-250 um; the invention is beneficial for an operator to determine whether the injection process of the ovum is finished and to quickly position the position of the ovum before and after the injection, thereby reducing the operation time and greatly reducing the operation difficulty.

Description

Novel single sperm microinjection ware

Technical Field

The invention relates to a single sperm microinjection technology, in particular to a novel single sperm microinjection dish.

Background

Due to the influence of social factors and environmental factors, the semen quality of men tends to decline year by year, and the male infertility caused by the factors is more and more. In the assisted reproduction technology, in order to solve the sterility problem caused by male factors, such as patients with severe oligospermia, weak teratospermia, the intracytoplasmic sperm injection (ICSI) technology is born. The technology is to inject a single sperm into an ovum by means of a micromanipulation system to fertilize the ovum, only a plurality of sperms are needed to achieve the aims of fertilization and pregnancy, and the technology is the most effective treatment method for patients with severe male infertility at present.

In the technology of single sperm microinjection, a microinjection dish is used as a carrier platform for storing sperms and eggs, then a fine micro holding needle is used for fixing the eggs on the dish, and a micromanipulation injection needle is used for injecting the braked single sperms into the cytoplasm of the eggs, so that the fertilization of the eggs by male sterile sperms is realized.

In the preparation of the traditional single sperm microinjection dish, the preparation method specifically comprises ovum injection microdroplets and needle washing microdroplets prepared by HEPES buffer solution and sperm braking microdroplets prepared by PVP. At present, the micro-injection dish used in clinic is mostly a plastic dish with a flat bottom surface, and the preparation of micro-droplets is generally carried out on the surface of the plastic dish. When preparing the ovum for injecting the micro-droplets, the circular micro-droplet method is generally adopted for preparation, and one ovum is placed in each micro-droplet. According to different operation habits of each experimenter, a plurality of circular buffer micro-droplets can be manufactured on the plastic plate, and the eggs in the buffer micro-droplets are injected one by one in sequence.

However, in actual practice, the following problems are often encountered:

1. when an experimenter carries out ICSI operation, because the injected ovum and the non-injected ovum have no difference in morphology, and meanwhile, the experimenter is concentrated in high intensity for a long time, whether the ovum in each buffer micro-droplet is injected or not can not be distinguished sometimes, and finally the ovum injection process is missed or the consequence of multiple injections is caused.

2. In the preparation process of the ovum buffer micro-droplets, the volume of the buffer micro-droplets determines the buffer effect of liquid, theoretically, the volume of the micro-droplets is not suitable to be too small, otherwise, the buffer effect of the liquid is influenced due to the evaporation of the micro-droplets. However, the volume of the micro-droplets is too large, the micro-droplets are shaken when the micro-injection dish is moved, and the micro-droplets float to other positions or are connected with other micro-droplets, so that the operation effect is influenced.

3. In the traditional ovum buffer micro-droplet, the ovum is loaded in the buffer micro-droplet, and the specific position of the ovum in the micro-droplet needs to be carefully searched under a high power microscope because the micro-droplet is not prepared too small, thereby increasing the working time of an operator and increasing the labor intensity.

Disclosure of Invention

The invention aims to provide a novel single sperm microinjection dish to solve the problems in the background art.

In order to achieve the purpose, the invention adopts the following technical scheme:

a novel single sperm microinjection dish comprises a base, wherein the upper surface of the base comprises a horizontal long plane, one side of the long plane along the width direction is an inclined plane with the bottom end connected with the long plane, and the other side of the long plane along the width direction is a vertical step higher than the long plane;

a plurality of cylindrical concave holes are formed in the inclined plane;

the diameter and the column height of the concave hole of the cylinder are both 200-250 um;

the width of the long strip plane is 200 um-250 um;

the long strip plane and the inclined plane are provided with baffles on two sides of the length direction of the long strip plane.

The width of the strip plane is 200 um.

A plurality of cylinder shrinkage pools all are located same water flat line.

The distance between the horizontal line of the concave hole of the cylinder and the bottom edge of the inclined plane is 500-800 um.

The included angle between the inclined plane and the horizontal plane is not more than 15 degrees.

The base can be a disc type base or a cuboid type base. When the base is a disc type base, the baffle is a baffle wall which is vertically connected with the edge of the disc type base and is higher than the highest point of the upper surface of the disc type base.

Compared with the prior art, the invention has the advantages that the width of the long strip plane is designed to be slightly larger than the diameter of a common ovum, so that the position of the ovum can be limited within a certain range after the ovum is loaded, an operator can quickly position the ovum, the operation time is reduced, and the operation difficulty is greatly reduced. And the eggs moved into the cylindrical concave hole are all injected eggs, so that the eggs are prevented from being injected or injected for multiple times. Due to the design of the inclined plane, the situation that the uninjected ovum on the long-strip plane falls into the concave hole of the cylinder due to the sliding of external force is avoided; due to the design of slight inclination, the phenomenon that the holding needle is broken in the process of translating the ovum due to the overlarge angle of the inclined plane is avoided.

Drawings

FIG. 1 is a perspective view of an injection dish of the present invention in an embodiment using a rectangular parallelepiped base (drawing of a baffle plate is omitted);

FIG. 2 is a schematic side view of the injection dish of the present invention in use;

fig. 3 is a top view of an injection dish of the present invention under an embodiment using a disk base.

In the figure, the device comprises a base 1, a base 2, an inclined plane 3, a cylindrical concave hole 4, a long plane 5, a vertical step 6, a micro-injection needle 7 and a micro-holding needle.

Detailed Description

The following describes embodiments of the present invention with reference to the drawings.

As shown in fig. 1 to 3, the novel single sperm microinjection dish of the present invention comprises a base 1, wherein the upper surface of the base 1 comprises a horizontal strip plane 4, one side of the strip plane 4 along the width direction is an inclined plane 2 with the bottom end connected to the strip plane 4, and the other side of the strip plane 4 along the width direction is a vertical step 5 higher than the strip plane 4;

a plurality of cylindrical concave holes 3 are formed in the inclined plane 2;

the diameter and the column height of the cylindrical concave hole 3 are both 200-250 um;

the width of the long strip plane 4 is 200 um-250 um;

the long strip plane 4 and the inclined plane 2 are provided with baffle plates on two sides of the long strip plane 4 in the length direction, and the baffle plates are used for preventing injection buffer solution from flowing out.

The width of the strip plane 4 is 200 um.

The plurality of cylindrical concave holes 3 are all located on the same horizontal line.

The distance between the horizontal line of the cylindrical concave hole 3 and the bottom edge of the inclined plane 2 is 500-800 um.

The included angle between the inclined plane 2 and the horizontal plane is not more than 15 degrees.

The diameter and the hole depth of the cylinder concave hole 3 are both larger than 200um and larger than the diameter of the ovum by about 150 um. The width of the long strip plane 4 is designed to be about 200um (slightly larger than the diameter of a common ovum), so that the position of the ovum can be limited within a certain range after the ovum is sampled, an operator can quickly position the ovum, the operation time is shortened, and the operation difficulty is greatly reduced.

The base of the invention can be a rectangular base or a disc base, as shown in fig. 1 and 3, respectively, and the drawing of the baffle plate or the baffle wall on the base is omitted in the drawing. A disc-type base is used in most cases.

When the injection device is used, eggs are firstly loaded to the long plane 4, the injection is carried out on ICSI by using the micro-injection needle 6, and then the injected eggs are slowly moved into the cylindrical concave hole 3 along the inclined plane 2 by using the micro-holding needle 7, so that the eggs moved into the cylindrical concave hole 3 are all the injected eggs, and the missed injection or multiple injections of the eggs are avoided. The design of the inclined plane 2 prevents the uninjected ovum on the long plane 4 from falling into the cylindrical concave hole 3 due to the sliding of external force; due to the slightly inclined design, the phenomenon that the holding needle is broken in the process of translating the ovum due to the overlarge angle of the inclined plane 2 is avoided. In the case of egg injection buffer loading, the buffer may be added to the strip plane, with all injected eggs in one large droplet. Therefore, the buffer solution can be ensured to be large enough, the buffer effect is not influenced by the volatilization of the liquid, and the change of the positions of the liquid drops at the buffer position is avoided. When the ovum in the cylindrical concave hole 3 is sucked, the ovum can be sucked out by a drawn Pasteur pipette.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.

Claims

1. A novel single sperm microinjection dish is characterized by comprising a base (1), wherein the upper surface of the base (1) comprises a horizontal long plane (4), one side of the long plane (4) along the width direction is an inclined plane (2) with the bottom end connected with the long plane (4), and the other side of the long plane (4) along the width direction is a vertical step (5) higher than the long plane (4);

a plurality of cylindrical concave holes (3) are formed in the inclined plane (2);

the diameter and the column height of the cylindrical concave hole (3) are both 200-250 um;

the width of the strip plane (4) is 200 um-250 um;

the long strip plane (4) and the inclined plane (2) are arranged on two sides of the length direction of the long strip plane (4) and are provided with baffles.

2. A novel single sperm microinjection dish according to claim 1, wherein said elongated plane (4) has a width of 200 um.

3. A novel single sperm microinjection dish according to claim 1, wherein a plurality of said cylindrical wells (3) are all located on the same horizontal line.

4. The novel single sperm microinjection dish of claim 1, wherein the distance between the horizontal line of the cylindrical concave hole (3) and the bottom edge of the inclined plane (2) is 500-800 um.

5. A novel single sperm microinjection dish according to claim 1, wherein said base (1) is a disk base.

6. The dish of claim 5, wherein the edge of the disc-type base is vertically connected to a wall that is higher than the highest point of the upper surface of the disc-type base.

7. A novel single sperm microinjection dish according to claim 1, wherein said bevel (2) forms an angle with the horizontal plane not greater than 15 °.