JP6686958B2 - Cell processing container - Google Patents

Description

  The present invention relates to a cell treatment container for use in treatment of cells using a treatment liquid, such as treatment of embryos immediately before transplantation.

  In the field of assisted reproductive medicine, fertilized eggs that have been cultured by in vitro fertilization are transplanted after undergoing a certain culture process, leading to implantation, pregnancy and childbirth. At that time, many improvements have been made to the culture dish and method used in the culture process, and efforts have been made to improve work efficiency and reduce the risk of fertilized egg loss due to mistakes. On the other hand, when a fertilized egg after culturing is transplanted into a female body, it is generally different from the composition of the culture solution at the time of culturing, whether it is a fresh embryo transfer or a freeze-thaw embryo transfer. After transferring to a dedicated culture medium (called “implantation medium”), the embryo is taken into the catheter and transplanted. At this time, in order to make the state immersed in the transplant solution as close to the body environment as possible, it is stably maintained in an incubator in which the temperature, humidity, gas concentration, etc. are controlled. The time until the embryo is aspirated with a catheter and transplanted becomes an environment in which temperature, humidity, and gas are different and stress is high for the embryo, so a short work is preferable.

  However, no dedicated container for performing the above-mentioned transplantation work has been provided so far. Therefore, each clinic is currently using a normal petri dish, a 4-well petri dish, a center well dish, and the like so that there is as little work error as possible and the transplantation work can be performed in a short time.

  Furthermore, since the patient is already waiting in the operation room during the transplantation process, the worker needs to work under a sense of tension in consideration of the risk of damaging the embryo due to a mistake in addition to the above short-time work. It was

  The work in the above environment is not easy when working in a wide range of wells because the catheter used is made of a soft material such as silicone. Further, when the depth of the transplanted liquid in the cell treatment container is insufficient, bubbles are likely to enter during suction work with a catheter. Furthermore, since the bottom surface is flat on the well of a normal petri dish, the embryo moves to the peripheral wall of the well or the end of the transplant fluid drop during movement of the petri dish or catheter work, and disappears under observation, or in the worst case, There was a risk of damaging or losing the embryo.

  In addition, in recent years, it has been reported that addition of various components at the time of transplantation affects implantation and pregnancy rate, and there is also a culture medium for transplantation containing a mixture of expensive additives. If there are many cases, the cost will increase, leading to an increase in treatment cost.

  Patent Document 1 discloses a device for monitoring and / or culturing at least two microscopic objects and a target medium, the device comprising at least one recess, and each recess further comprising: It has a depression with a smaller cross section than the depression. The microscope object and object medium are retained in the depression. The device described in Patent Document 1 is suitable for the purpose of observing cells, but it is clear that it is not easy to take out the cells retained in the recess.

Patent Document 2 discloses a cell culture container having a recess for containing cells and a culture solution. Patent Document 2 describes that the bottom surface of the recess in the container is preferably 0.75 mm ² or more and has a relatively large area, and in Patent Document 2, the shape of the bottom surface of the recess in plan view. Is not specified. When cells and the like are stored in a conventional cell culture container as described in Patent Document 2, it is difficult to specify the position of the cells and the like on the bottom surface, and therefore, when observing with a microscope during the working process. First, it is necessary to confirm a wide field of view at a certain low magnification (for example, 20 cultures or less) to identify the position of cells and the like, and there is a risk that workability is reduced. Further, in some cases, even when observing at the low magnification, there is a cell to be handled outside the visual field range, and there is a risk of disappearing or a decrease in workability. Patent Document 2 does not disclose means for avoiding such a risk. Further, Patent Document 2 does not disclose any means for facilitating the handling of cells contained in the concave portion of the container using a catheter having a small outer diameter formed of a soft material such as silicone.

Japanese Patent No. 5143227 JP, 2014-207897, A

  The present invention provides a method for treating cells (including multicellular bodies such as embryos) with a treatment solution such as a treatment with a culture solution for transplantation of embryos before transplantation, which shows damage to cells or a microscopic observation field. The purpose is to solve the problem of disappearance or difficulty of handling with a catheter.

Therefore, the present invention discloses the following inventions as means for solving the above problems.
(1) The bottom of the container,
A cell treatment container provided with one or more accommodating portions that are provided at the bottom of the container and in which an accommodation space for accommodating cells and a cell treatment liquid is formed and open upward,
The volume of the accommodation space is 200 μl or more and 1000 μl or less,
The formation surface, which is a surface that forms the accommodation space, of the accommodation portion includes a deepest portion that is a deepest portion of the accommodation portion and an inclined surface that is formed so as to surround the deepest portion. Have,
The inclined surface is continuous with the deepest part,
The deepest part is formed so as to fit within a range of a circle having a diameter of 3 mm in a plan view of the cell treatment container.
The cell treatment container.

  In the above (1), the surface of the container bottom portion and the outer wall surface of the housing portion may be connected by a rounded curved surface. The curved surface may have a radius of curvature of 10 μm to 15 mm, preferably 10 μm to 1 mm. The curved surface is preferably a concave curved surface.

In the above (1), the formation surface of the accommodation portion includes an accommodation portion bottom surface that forms a bottom of the accommodation space, and an accommodation portion side surface that stands up from a peripheral edge of the accommodation portion bottom surface and surrounds the accommodation space,
The housing bottom surface has the inclined surface,
The bottom surface of the housing portion and the side surface of the housing portion may be connected by a rounded curved surface. The curved surface may have a radius of curvature of 10 μm to 15 mm, preferably 10 μm to 1 mm. The curved surface is preferably a concave curved surface.

In the above (1), the formation surface of the accommodation portion includes an accommodation portion bottom surface that forms a bottom of the accommodation space, and an accommodation portion side surface that stands up from a peripheral edge of the accommodation portion bottom surface and surrounds the accommodation space,
The housing bottom surface has the inclined surface,
The side surface of the accommodating portion may be a surface having an inclination angle of less than 90 ° or a surface having an inclination angle of 90 ° or more as defined herein.

  In the above (1), the outer wall surface of the accommodating portion may be a surface having an inclination angle of less than 90 ° or a surface having an inclination angle of 90 ° or more as defined herein.

In the above (1), the formation surface of the accommodation portion includes an accommodation portion bottom surface that forms a bottom of the accommodation space, and an accommodation portion side surface that stands up from a peripheral edge of the accommodation portion bottom surface and surrounds the accommodation space,
The housing bottom surface has the inclined surface,
A width between the side surface of the accommodating portion and an outer wall surface of the accommodating portion among wall surfaces forming the accommodating portion may be 0.3 mm to 3 mm.

  In (1) above, the thickness of the bottom of the container may be 0.3 mm to 3 mm.

  In the above (1), one or more markings capable of identifying the direction of the cell treatment container may be provided. The marking can be provided on the bottom of the container. The marking can be configured to be visually and / or tactilely distinguishable.

  In the above (1), at least one micro tag having readable information recorded thereon may be coupled to the cell treatment container.

  The cell treatment container of (1) further includes a lid, and at least one micro tag is coupled to the lid and the container body that is a portion other than the lid.

  In the above (1), the surface of the container bottom may be formed such that the depth in the container increases near the periphery of the container bottom.

In (1) above, the height of the entire cell treatment container in the vertical direction may be 1 mm to 15 mm.
(2) The cell treatment container according to (1), which includes an outer peripheral wall portion standing upright from a peripheral edge of the container bottom portion.

  In the above (2), the surface of the bottom of the container sandwiched by the accommodating part and the outer peripheral wall part and the outer wall surface of the accommodating part may be connected by a rounded curved surface. The curved surface may have a radius of curvature of 10 μm to 15 mm, preferably 10 μm to 1 mm. The curved surface is preferably a concave curved surface.

  In the above (2), the surface of the container bottom, which is sandwiched between the accommodation portion and the outer peripheral wall, and the inner peripheral surface of the outer peripheral wall may be connected by a rounded curved surface. it can. The curved surface may have a radius of curvature of 10 μm to 15 mm, preferably 10 μm to 1 mm. The curved surface is preferably a concave curved surface.

  In (2) above, among the wall surfaces forming the outer peripheral wall portion, the width between the inner peripheral surface of the outer peripheral wall portion and the outer peripheral surface of the outer peripheral wall portion may be 0.3 mm to 3 mm. .

  In (2) above, the outer peripheral surface of the outer peripheral wall portion is a surface on which at least one step is formed.

  In the above (2), one or more markings capable of identifying the direction of the cell treatment container may be provided. The marking may be provided on at least one selected from the container bottom and the outer peripheral wall. The marking can be configured to be visually and / or tactilely distinguishable.

  In the above (2), the outer peripheral surface of the outer peripheral wall portion may have a shape recessed inward.

In the above (2), the surface of the bottom of the container, which is sandwiched between the accommodating portion and the outer peripheral wall, is formed so that the depth in the container increases in the vicinity of the outer peripheral wall. it can.
(3) The cell processing container according to (1) or (2), wherein the inclined surface is formed from the peripheral edge of the formation surface to the deepest part.
(4) The cell treatment container according to any one of (1) to (3), wherein the height of the formation surface from the deepest part to the upper end is 2 mm or more and 12 mm or less.
(5) When the cell treatment container is placed on a horizontal plane, a virtual straight line connecting one point included in the deepest part on the formation surface and one point included in the upper end of the formation surface is formed. The cell treatment container according to any one of (1) to (4), wherein the smallest acute angle formed with the horizontal plane is 45 ° or less.
(6) The cell treatment container according to any one of (1) to (5), wherein the opening of the accommodation portion has a shape that is long in one direction in a plan view of the cell treatment container.
(7) The cell treatment container according to (6), wherein the opening of the accommodation portion has a width in the minor axis direction of 3 mm or more and 15 mm or less in a plan view of the cell treatment container.
(8) The opening of the accommodation portion has a shape in which a first portion and one or more second portions extending in at least one direction from the first portion are combined in a plan view of the cell treatment container. The cell treatment container according to (1) to (7).
(9) A cell holding region for holding cells is formed by at least one of the deepest portion of the formation surface and a portion of the inclined surface adjacent to the deepest portion,
The cell treatment container according to any one of (1) to (8), wherein the inclined surface is an inclined surface that is inclined at a constant gradient in the cell holding region.
(10) The cell treatment container according to (9), wherein the surface roughness of the inclined surface in the cell holding region is less than 4.0 μm in maximum height Ry value.
(11) A cell holding region for holding cells is formed by at least one of the deepest portion of the formation surface and a portion of the inclined surface adjacent to the deepest portion,
The cell treatment container according to any one of (1) to (10), wherein in the cell holding region, an angle formed between the facing portions of the inclined surface is larger than 90 °.
(12) The forming surface includes a bottom surface of the housing portion that forms a bottom of the housing space, and a side surface of the housing portion that stands up from a peripheral edge of the bottom surface of the housing portion and surrounds the housing space.
The housing bottom surface has the inclined surface,
The cell treatment according to any one of (1) to (11), wherein an angle formed by the bottom surface of the housing and the side surface of the housing is greater than 90 ° at a portion where the bottom surface of the housing portion and the side surface of the housing portion intersect. container.
(13) Any one of (1) to (12), wherein the container bottom portion is further provided with one or more container-shaped portions in which a container-shaped portion accommodating space for accommodating cells and / or liquid is formed. The cell treatment container according to the item 1.

The present specification further discloses the following inventions.
(14) The bottom of the container,
An outer peripheral wall portion standing up from the peripheral edge of the container bottom,
A cell treatment container provided with one or more accommodating portions that are provided at the bottom of the container and in which an accommodation space for accommodating cells and a cell treatment liquid is formed and open upward,
The container further comprises two or more partition walls that stand upright from the bottom of the container and bridge the storage portion and the outer peripheral wall portion,
A container-shaped part accommodation space for accommodating cells and / or liquid, which is surrounded by the container part, the outer peripheral wall part, and an adjacent pair of the two or more partition walls, with the container bottom part as a bottom part. The cell treatment container, wherein one or more container-shaped portions in which the above are formed are formed.

  In (14) above, a surface of the partition wall facing the container-shaped portion accommodation space and a surface of the container bottom facing the container-shaped portion accommodation space may be connected by a rounded surface. The curved surface may have a radius of curvature of 10 μm to 15 mm, preferably 10 μm to 1 mm. The curved surface is preferably a concave curved surface.

  In (14) above, a surface of the partition wall facing the container-shaped portion accommodation space and an outer wall surface of the accommodation portion may be connected by a rounded surface. The curved surface may have a radius of curvature of 10 μm to 15 mm. The curved surface is preferably a concave curved surface.

  In (14) above, a surface of the partition wall facing the container-shaped portion accommodation space and an inner peripheral surface of the outer peripheral wall portion may be connected by a rounded surface. The curved surface may have a radius of curvature of 10 μm to 15 mm. The curved surface is preferably a concave curved surface.

  In (14), the surface of the partition wall facing the container-shaped portion accommodation space may be a surface having an inclination angle of less than 90 ° defined in the present specification, or a surface of 90 ° or more.

  In (14), the partition wall may have a thickness of 0.3 mm to 3 mm.

  In the above (14), one or more further container shapes in which a container-shaped part accommodation space for accommodating cells and / or liquid is formed in a part of the container bottom facing the container-shaped part accommodation space. The part can be formed.

  In the above (14), the height d of the portion of the partition wall that stands up from the bottom of the container can be 1 mm or more, preferably 2 mm or more, and more preferably 15 mm or less.

  The cell treatment container according to (14) further includes an outer lid portion, and the height d of the portion of the partition wall that stands up from the container bottom portion, the height e of the portion of the storage portion that stands up from the container bottom portion, and The height f of the portion of the outer peripheral wall portion standing upright from the bottom of the container satisfies the relations of f ≧ d and f ≧ e, and the surface of the outer lid portion facing into the container at the time of mounting is the upper end of the outer peripheral wall portion. The following conditions can be further satisfied in the case of contacting with.

    (I) One surface of the partition wall is a surface having an affinity for a liquid contained in a container-shaped portion accommodation space surrounded by the surface, and the surface of the outer lid portion facing into the container when mounted. However, when the surface has an affinity for the liquid, d has a value smaller than f by 1 mm or more.

    (II) One surface of the partition wall is a surface having an affinity for a liquid contained in the container-shaped portion accommodation space surrounded by the surface, and the surface of the outer lid portion facing into the container when mounted. Is a surface having no affinity for the liquid, d is a value of f or less.

    (III) When one surface of the partition wall is a surface that does not have an affinity for the liquid contained in the container-shaped portion accommodation space surrounded by the surface, d is a value of f or less.

In (I), (II) and (III), the lower limit of d is not particularly limited, but it is preferably a value larger than 2 mm, more preferably 3 mm or more, and further preferably 8 mm or more.
(15) The upper end of the accommodating portion and the upper ends of the two or more partition walls are independently located at the same vertical position as the upper end of the outer peripheral wall portion, or from the upper end of the outer peripheral wall portion. The cell treatment container according to (14) above, which is also below.
(16) The cell treatment container according to (14), wherein an upper end of the outer peripheral wall is located above an upper end of the accommodating portion and an upper end of each of the two or more partition walls.
(17) The cell treatment container according to (14), wherein an upper end of the accommodating portion is at the same vertical position as an upper end of the outer peripheral wall portion, or is located higher than an upper end of the outer peripheral wall portion.
(18) The cell treatment container according to (14), wherein an upper end of the outer peripheral wall portion is above an upper end of the accommodating portion.
(19) The upper end of the accommodating portion and at least a pair of adjacent upper ends of the two or more partition walls independently have the same vertical position as the upper end of the outer peripheral wall portion, or The cell treatment container according to (14) above, which is above the upper end of the outer peripheral wall.
(20) The cell treatment container according to (14), wherein an upper end of the outer peripheral wall is above at least one selected from an upper end of each of the two or more partition walls and an upper end of the accommodating portion.
(21) The cell treatment container according to (14), wherein at least one selected from an upper end of each of the two or more partition walls and an upper end of the accommodating portion is above an upper end of the outer peripheral wall portion.
(22) Three or more partition walls bridging at least one of the accommodating portions and the outer peripheral wall portion are provided,
The three or more partition walls include one or two or more adjacent partition walls A, and a pair of partition walls B adjacent to the partition wall A,
The upper end of each of the pair of partition walls B is higher than the upper end of each of the partition walls A,
The cell treatment container according to (14), wherein both the upper end of the accommodation portion and the upper end of the outer peripheral wall portion are above the respective upper ends of the partition walls A.
(23) The cell treatment container according to any one of (14) to (22), wherein the container further includes the feature described in any one of (1) to (13).

  In the present invention, the notation "numerical value 1-numerical value 2" in relation to the numerical value range means a numerical value range including numerical value 1 and numerical value 2 at both ends, with numerical value 1 being the lower limit value and numerical value 2 being the upper limit value, It is synonymous with "numerical value 2 or less".

  The present specification includes the disclosures of Japanese Patent Application No. 2015-105796 and Japanese Patent Application No. 2015-156472, which are the basis of priority of the present application.

  According to the present invention, it is possible to suppress damage to cells or disappearance from a microscopic observation visual field, or it is easy to handle cells using a catheter or pipette. A processing vessel is provided.

It is a schematic diagram of the top view of the cell treatment container 100 of Embodiment 1 of this invention. It is a schematic diagram of the XX cross section of the cell treatment container 100 shown in FIG. 1A. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 1A of the cell treatment container 100 of Embodiment 1 further provided with the outer lid part 170. The cell 10 and the cell treatment liquid 20 are accommodated in the accommodation space 150 of the cell treatment container 100 of the first embodiment, and the operation is performed by covering the cells 10 and the cell treatment liquid 20 with the outer lid portion 170 and corresponding to the XX cross section in FIG. 1A. It is a schematic diagram of a cross section. FIG. 3B is a schematic cross-sectional view corresponding to the XX cross section in FIG. 1A, showing an operation of handling the cells 10 stored in the storage space 150 of the cell treatment container 100 of Embodiment 1 with the catheter 30. It is a figure explaining the definition of inclination-angle (theta) ₁ of the surface in this invention. It is a figure explaining the definition of the angle (theta) _{2 which} the parts which the slanting surface opposes in the cell holding area | region 146 of the cell processing container 100 in this invention. It is a schematic diagram of a cross section of an example of the cell holding region 146. (A) It is a schematic diagram of a cross section of another example of the cell holding region 146. (B) A schematic view of a plan view of the example shown in (a) of the cell holding region 146. It is a schematic diagram for explaining angles θ _{4 and} θ ₅ in the present invention. It is a schematic diagram of the top view of the cell treatment container 100 of Embodiment 2 of this invention. It is a schematic diagram of the XX cross section of the cell treatment container 100 shown in FIG. 2A. It is a schematic diagram of the top view of the cell treatment container 100 of Embodiment 3 of this invention. It is a schematic diagram of the XX cross section of the cell treatment container 100 shown in FIG. 3A. It is a schematic diagram of the top view of the cell treatment container 100 of Embodiment 4 of this invention. It is a schematic diagram of the XX cross section of the cell treatment container 100 shown in FIG. 4A. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 1A of the cell treatment container 100 of Embodiment 5 of this invention. It is a schematic diagram of the top view of the cell processing container 100 of Embodiment 6 of this invention. It is a schematic diagram of the YY cross section of the cell treatment container 100 shown in FIG. 6A. It is a schematic diagram of the cross section corresponding to the YY cross section in FIG. 6A, which shows the operation of culturing the cells 630 in the culture container-shaped portion 610 using the cell treatment container 100 of Embodiment 6 of the present invention. It is a schematic diagram which shows the shape of the microwell 615 in the cell treatment container 100 of Embodiment 6 of this invention. FIG. 16 is a schematic diagram showing an example of a microwell 615 in which two or more line-shaped concave portions 6154 and two or more line-shaped convex portions 6155 are alternately formed on the microwell side surface 6152. FIG. 6B is a schematic diagram of a plan view of the microwell 615 shown in FIG. 6E. It is a schematic diagram which shows an example of the microwell 615 in which two or more dot-shaped protrusions 6156 were formed on the microwell side surface 6152. It is a schematic diagram of the top view of the cell treatment container 100 of Embodiment 7 of this invention. It is a schematic diagram of the XX cross section of the cell treatment container 100 shown in FIG. 7A. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 8 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 9 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 10 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 11 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 12 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 13 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 14 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 15 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 16 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 17 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 18 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 19 of this invention. FIG. 7B is a schematic view of a cross section corresponding to the XX cross section in FIG. 7A in a state where the cell treatment container 100 according to the eighth embodiment of the present invention is covered with the outer lid portion 170. FIG. 7B is a schematic view of a cross section corresponding to the XX cross section in FIG. 7A in a state where the cell processing container 100 of Embodiment 11 of the present invention is covered with the inner lid portion 210. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A in the state which covered the cell processing container 100 of Embodiment 9 of this invention with the outer lid part 170. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 20 of this invention. It is a schematic diagram of the cross section corresponded to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 21 of this invention. It is a schematic diagram of the cross section corresponding to the XX cross section in FIG. 7A of the cell treatment container 100 of Embodiment 22 of this invention. It is a schematic diagram of the top view of the cell processing container 100 of the modification 1 of Embodiment 1 of this invention. It is a schematic diagram of the top view of the cell processing container 100 of Embodiment 1 modification 2 of this invention. It is a schematic diagram of the end surface corresponded to the AA end surface of FIG. 7A of the cell treatment container 100 of the modification 1 of Embodiment 7 of this invention. It is a schematic diagram of the end surface corresponded to the AA end surface of FIG. 7A of the cell treatment container 100 of the modification 2 of Embodiment 7 of this invention. It is a schematic diagram of the end surface corresponded to the AA end surface of FIG. 7A of the cell treatment container 100 of the modification 3 of Embodiment 7 of this invention. It is a schematic diagram of the end surface corresponded to the AA end surface of FIG. 7A of the cell treatment container 100 of the modification 4 of Embodiment 7 of this invention. It is a schematic diagram of the end surface corresponded to the BB end surface of FIG. 7A of the cell treatment container 100 of the modification 5 of Embodiment 7 of this invention. FIG. 7B is a plan view of a region corresponding to a region 310 in FIG. 7A of the cell treatment container 100 according to the modified example 6 of the seventh embodiment of the present invention. It is a schematic diagram of the end surface corresponded to the BB end surface in FIG. 7A of the cell treatment container 100 of the modification 7 of Embodiment 7 of this invention. It is a schematic diagram of the end surface corresponded to the BB end surface of FIG. 7A of the cell treatment container 100 of the modification 8 of Embodiment 7 of this invention. It is a schematic diagram of the end surface corresponded to the BB end surface of FIG. 7A of the cell treatment container 100 of the modification 9 of Embodiment 7 of this invention. An example in which the cell treatment container 100 of Embodiment 7 of the present invention includes an outer lid portion 170 is shown. It is a top view of the cell treatment container 100 of Embodiment 10 modification 10 of this invention. It is a schematic diagram of the end surface corresponded to CC end surface of FIG. 7A of the cell treatment container 100 of the modification 11 of Embodiment 7 of this invention. It is a schematic diagram of the end surface corresponded to CC end surface in FIG. 7A of the cell treatment container 100 of the modification 12 of Embodiment 7 of this invention. The top view of the cell processing container 100 of this invention which concerns on the modification 13 of Embodiment 7 is shown. FIG. 37C is a cross-sectional schematic diagram of the cell processing container 100 of the present invention according to the thirteenth modification of the seventh embodiment, which is taken along the line DD of FIG. 37A. FIG. 3 is a perspective view of an outer peripheral wall portion 120 of the cell treatment container 100 in the vicinity of a recessed portion 371. The top view of the cell processing container 100 of this invention which concerns on the modification 14 of Embodiment 7 is shown. The side view of the cell processing container 100 of this invention which concerns on the modification 15 of Embodiment 7 is shown. The top view of the cell processing container 100 of this invention which concerns on the modification 16 of Embodiment 7 is shown. FIG. 40B shows an end view of the EE end surface in FIG. 40A. The top view of the cell treatment container 100 of this invention which concerns on the modification 17 of Embodiment 7 is shown. FIG. 41B is an end view of the container bottom portion 110 in the vicinity of the identification circles 411 to 416 in the cell treatment container 100 of the present invention according to Modification 17 of the seventh embodiment shown in FIG. 41A. The top view of the cell processing container 100 of this invention which concerns on the modification 18 of Embodiment 7 is shown. The top view of the cell processing container 100 of this invention which concerns on the 19th modification of Embodiment 7. The top view of the cell processing container 100 of this invention which concerns on the 20th modification of Embodiment 7 is shown. An example in which a micro tag 451 is bonded to the inner surface of the recessed portion 371 of the cell treatment container 100 of the present invention according to the thirteenth modification of the seventh embodiment (FIGS. 37A, 37B, 37C) is shown. The example which combined the micro tag 461 on the container bottom lower surface 113 of the container bottom 110 of the cell treatment container 100 of this invention which concerns on the 13th modification of Embodiment 7 (FIG. 37A, 37B, 37C) is shown. 1 is a schematic view of a cross section corresponding to the XX cross section in FIG. 1A of a modification of the cell treatment container 100 according to the first embodiment of the present invention in which the outer peripheral wall 120 is partially recessed toward the inner side in the radial direction. Show. A schematic view of a cross section corresponding to the XX cross section in FIG. 1A of a modified example of the cell treatment container 100 of the present invention according to the first embodiment in which the central portion of the container bottom portion 110 partially projects into the container. Show. A modification of the cell treatment container 100 of the present invention according to the first embodiment, in which the outer peripheral wall portion 120 is partially recessed radially inward and the central portion of the container bottom portion 110 is partially protruded into the container, The schematic diagram of the cross section corresponding to the XX cross section in FIG. 1A is shown.

  In the present invention, the "up and down direction" means that when a liquid such as a culture liquid is stored in the storage space in a state where the cell treatment container of the present invention is placed on a horizontal surface, the depth direction of the liquid (that is, the vertical direction) Points in the matching direction. And in the present invention, the direction from the bottom of the cell treatment container, the containing portion or the container-like portion to the opening is "upward" along the vertical direction, and the direction from the opening of the containing portion to the bottom is "downward" along the vertical direction. And

  When the cell treatment container of the present invention is placed on a horizontal surface, the surface that comes into contact with the horizontal surface is referred to as the “container lower surface”, and in the illustrated embodiment is the container lower surface 112. The bottom surface 112 of the container is a continuous flat surface in the illustrated embodiment, but is not limited to this, and may be composed of one or more point-like or linear surfaces capable of contacting the horizontal surface (for example, Modification 13) of Embodiment 7 shown in FIGS. 37A, 37B, 37C. In this case, the plane including the surface that contacts the horizontal plane can be regarded as the bottom surface of the container. Then, in the cell treatment container of the present invention, the distance in the vertical direction from one site A to another site B may be referred to as the “height” from the site A to the site B. When one of the two parts included in the cell treatment container has a distance from the lower surface of the container along the vertical direction that is larger than the distance of the other part from the lower surface of the container along the vertical direction. , One part is also said to be "upper," "upper," or "upper" than the other part, and the other part is "lower", "lower side", or "more than one part". Say "below" or located. In addition, in the site included in the cell treatment container of the present invention, the end of the site on the cross section along the up-down direction that is away from the lower surface of the container is referred to as the “upper end” of the site.

  In the present invention, the peripheral edge of the bottom of the container refers to a portion that becomes the outer contour of the bottom of the container when the cell treatment container of the present invention is placed on a horizontal surface and viewed in a plan view.

In the present invention, the inclination angle of the surface is defined as follows. As shown in FIG. 1F, a normal line passing through a point P on the surface S which is the surface of the member X is N, and an imaginary straight line passing through the point P in the vertical direction (as defined above) is L, When the half straight line portion of the normal line N on the side where the member X does not exist from the point P is N ₁ and the half straight line portion of the virtual straight line L on the upper side from the point P is L ₁ , the normal line N and the virtual straight line Among the angles formed by L, the angle θ ₁ formed between the half straight line portion N ₁ , the point P, and the half straight line portion L ₁ is defined as the inclination angle at the position of the point P of the surface S. .

  The cell treatment container of the present invention can be widely used for the purpose of treating cells using a cell treatment liquid. The treatment of cells includes various treatments of cells performed in a liquid such as cell culturing, washing, and thawing.

  The cells to be treated using the cell treatment container of the present invention and observed as necessary are not particularly limited, but include, for example, fertilized eggs, egg cells, ES cells (embryonic stem cells) and iPS cells (induced pluripotent stem cells). ) Is mentioned. The egg cell refers to an unfertilized egg cell and includes an immature oocyte and a mature oocyte. After fertilization, the number of fertilized eggs increases at the 2-cell stage, 4-cell stage, and 8-cell stage due to cleavage, and then develops into blastocysts through the morula. Fertilized eggs include early embryos such as 2-cell embryos, 4-cell embryos and 8-cell embryos, morula, blastocysts (including early blastocysts, expanded blastocysts and escape blastocysts). Blastocyst refers to an embryo that consists of an outer cell that has the potential to form a placenta and an inner cell mass that has the potential to form an embryo. Among the fertilized eggs listed here, ones just before transplantation to the mother are referred to as embryos for transplantation in the present specification. ES cells refer to undifferentiated pluripotent or totipotent cells obtained from the inner cell mass of blastocysts. The iPS cells refer to cells that have pluripotency similar to ES cells by introducing several kinds of genes (transcription factors) into somatic cells (mainly fibroblasts). That is, in the present invention, the cells to be treated also include an aggregate of a plurality of cells such as a fertilized egg and a blastocyst. The cell treatment container 100 of the present invention is suitable for treating mammalian and avian cells, particularly mammalian cells. Mammals refer to warm-blooded vertebrates, for example, primates such as humans and monkeys, rodents such as mice, rats and rabbits, companion animals such as dogs and cats, and domestic animals such as cows, horses and pigs. Can be mentioned. The cell treatment container of the present invention is particularly suitable for treatment of human or bovine fertilized eggs.

  As the cell treatment liquid, a culture liquid selected according to the cells, a transplant liquid that is a dedicated culture liquid for processing the embryo for transplant immediately before the transfer to the mother, a liquid for cell washing, a liquid for cell lysis, etc. Is mentioned.

  The material of the cell treatment container of the present invention is not particularly limited. Specifically, inorganic materials such as metal, glass, and silicon, plastics (for example, polystyrene resin, polyethylene resin, polypropylene resin, ABS resin, nylon, acrylic resin, fluorine resin, polycarbonate resin, polyurethane resin, methylpentene resin, Examples thereof include organic materials represented by phenol resin, melamine resin, epoxy resin, vinyl chloride resin, polyester resin). The cell treatment container of the present invention preferably contains one or more plastic materials, and more preferably one or more plastic materials. It is preferable that the plastic material is a material that satisfies the standard value of the dissolution test described in the Japanese Pharmacopoeia, for example, Japanese Pharmacopoeia, General Test Method, 7.02 Plastic Drug Container Test Method, 1.2 Dissolution Test, (Iv) A material that has been subjected to the procedure described in the ultraviolet absorption spectrum and has an absorbance of 0.08 or less at a wavelength of 220 nm to less than 241 nm and an absorbance of 0.05 or less at a wavelength of 241 nm to 350 nm in the ultraviolet absorption spectrum. Is preferred. The cell treatment container of the present invention can be manufactured by a method known to those skilled in the art. For example, when a cell treatment container made of a plastic material is manufactured, it can be manufactured by a conventional molding method such as injection molding.

  Add additives such as bluing agents and various pigments used in the production of transparent plastic molded products to the plastic materials by a masterbatch method, a dry blend method, a kneading method, a surface coating method, etc. The desired transparency and color combination can be imparted thereby. Therefore, a clear image can be obtained when observing the cells contained in the cell treatment container of the present invention.

  An additive such as an antistatic agent or an antistatic agent used in the production of plastic molded products can be added to the plastic material by a masterbatch method, a dry blending method, a kneading method, a surface coating method, or the like, As a result, it is possible to prevent the plastic product from being charged. Therefore, it is possible to prevent adhesion of dirt to the cell treatment container of the present invention due to static electricity and electric shock injury in the molding line.

  For observation with an optical microscope, the cell treatment container of the present invention is preferably made of a light transmissive material.

  The cell treatment container of the present invention may be surface-treated or surface-coated to promote the development of fertilized eggs. In particular, when co-culturing with cells of other organs (eg, endometrial cells or oviductal epithelial cells) to promote the development of fertilized eggs, these cells should be adhered to the surface of the cell treatment container in advance. Need to let. In such a case, it is advantageous to coat the surface of the cell treatment container with a cell adhesive material.

  The produced cell treatment container of the present invention preferably satisfies the standard value of the dissolution test described in the Japanese Pharmacopoeia. For example, the procedure described in Japanese Pharmacopoeia, General test method, 7.02 Plastic drug container test method, 1.2 Elution test, (iv) Ultraviolet absorption spectrum is carried out, and the wavelength in the ultraviolet absorption spectrum is 220 nm or more and less than 241 nm. It is preferable that the absorbance at 0.08 or less and the absorbance at a wavelength of 241 nm or more and 350 nm or less be 0.05 or less. It is preferable that the entire cell treatment container of the present invention has this characteristic, and it is more preferable that the inner surface of the container has this characteristic.

  The manufactured cell treatment container of the present invention is preferably sterilized by high-pressure steam sterilization, ethylene oxide sterilization, radiation sterilization, etc., and preferably sterilized by radiation sterilization. The sterilization dose for radiation sterilization may be 10 kGy to 100 kGy, and is preferably 15 kGy to 40 kGy.

  The amount of endotoxin in the produced cell treatment container of the present invention is preferably 20 EU (endotoxin unit) / container or less, and more preferably 2.15 EU (endotoxin unit) / container or less. The amount of endotoxin can be measured by the method described in Japanese Pharmacopoeia 4.01 Endotoxin Test Method, USP <85>.

Hereinafter, the structure of the cell treatment container of the present invention will be described with reference to the embodiments shown in the drawings, but the scope of the present invention is not limited to these embodiments. In the drawings of this specification, configurations having the same function are denoted by the same reference numerals regardless of the size and shape of each configuration, and description thereof is omitted except for special differences.
<Embodiment 1 (FIGS. 1A to 1J)>
1A to 1J show a first embodiment of a cell treatment container 100 of the present invention.

  The cell treatment container 100 includes a container bottom portion 110, an outer peripheral wall portion 120 standing upright from the peripheral edge of the container bottom portion 110, and one or more accommodating portions 130 provided in the container bottom portion 110. In all of the embodiments described in the present specification, only one container 130 is provided in the cell treatment container 100, but the number of the container 130 is 2 or more, for example, 2 to 9, preferably 2 to 10. It may be provided on the four container bottoms 110.

  The accommodating portion has a structure in which an accommodating space for accommodating cells and a cell treatment solution is formed and opened upward. The container may be formed in any manner, but typically, it can be formed by the container peripheral wall 140 standing upright from the container bottom 110 like the container 130 shown in the figure. The accommodating part peripheral wall part 140 includes a forming surface 141 that is a surface that forms the accommodating space 150 on the inner side, and an accommodating part outer wall surface 144 that faces the outer side of the accommodating space 150 on the outer side, and has an accommodating part opening side end An upper end 145 of the housing portion is provided. The upper end 145 of the housing portion does not have to be a flat upper end surface as shown, but may be a curved surface or a ridge line.

  The cell treatment container 100 has an opening formed by the outer peripheral wall inner peripheral surface 121 of the outer peripheral wall 120 and opening upward. As shown in the figure, the cell treatment container 100 preferably has a dish shape as a whole. The upper end of the outer peripheral wall portion 120 is the outer peripheral wall portion upper end 122. The outer peripheral wall upper end 122 does not have to be a flat upper end surface as shown in the drawing, and may be a curved surface or a ridge line.

  The shape of the figure when the inner peripheral side contour and / or the outer peripheral side contour of the outer peripheral wall upper end 122 is viewed in plan view can be any shape such as a circular shape (including a circular shape and an elliptical shape), and is preferable. Is circular. Opening width of the cell treatment container (in a figure when the inner peripheral side contour of the upper end 122 of the outer peripheral wall is viewed in a plan view, between a pair of points on the peripheral edge of the figure, which are opposed to each other with the center of gravity of the figure therebetween. The maximum value of the distance) is preferably 15 mm to 100 mm, more preferably 30 mm to 70 mm, and it is even more preferable that the size is almost the same as the size often found in existing petri dishes such as the diameter of 35 mm or 60 mm.

  The container bottom 111 which is the upper surface of the container bottom 110, the outer peripheral wall inner peripheral surface 121 which is the inner peripheral surface of the outer peripheral wall 120, and the housing outer wall surface 144 form an outer peripheral space 160 that is closed downward and opened upward. To be done.

  As shown in FIG. 1C, the cell treatment container 100 of the present embodiment may further include a detachable outer lid portion 170 capable of closing the opening of the cell treatment container 100.

  Next, the features of the housing section 130 will be described in detail.

  The forming surface 141 of the accommodating portion 130 includes a deepest portion 148 that is the deepest portion along the vertical direction of the accommodating portion 130, and an accommodating portion bottom inclined surface 142 that is formed so as to surround the deepest portion 148. And the accommodation portion bottom inclined surface 142 is continuous with the deepest portion 148. Only one deepest portion 148 exists in the formation surface 141 in plan view. In the present invention, the inclined surfaces such as the accommodating portion bottom inclined surface 142 are more preferably formed from the peripheral edge of the forming surface 141 to the deepest portion 148 as in the illustrated embodiments. Here, the “periphery” of the forming surface 141 is formed when the forming surface 141 observed through the opening of the container 130 is projected onto the horizontal surface while the cell treatment container 100 is placed on the flat horizontal surface. A part of the forming surface 141 that is the outer periphery of the figure. In the present invention, in the expression that “the inclined surface is formed from the peripheral edge of the forming surface to the deepest portion”, the inclined surface such as the accommodation portion bottom inclined surface 142 is the deepest portion from the peripheral edge of the forming surface 141. It suffices that it is formed over 148, and it is not necessary that the depth of the accommodating portion 130 increases as it approaches the deepest portion in plan view in all the portions of the inclined surface. For example, as long as the purpose of the present invention of holding cells in the cell holding area 146 described later is not impaired, the inclined surfaces such as the storage section bottom inclined surface 142 are in a direction perpendicular to the vertical direction defined above (that is, , The horizontal direction) may include a stepped portion including a part thereof, and a portion in which the depth of the accommodating portion 130 becomes shallower toward the deepest portion in a plan view.

  In the illustrated first embodiment, the forming surface 141 includes a housing portion bottom surface 400 that is a surface that forms the bottom of the housing space 150, and a housing portion side surface 143 that stands up from the peripheral edge of the housing portion bottom surface 400. Of these, the bottom surface 400 of the housing portion includes a deepest portion 148 and a bottom sloped surface 142 of the housing portion that is formed so as to surround the deepest portion 148 and that is continuous with the deepest portion 148. Of the portion of the forming surface 141 corresponding to the peripheral edge in the plan view defined above, the upper edge is the forming surface upper end 149. In the first embodiment, at least the housing portion bottom inclined surface 142 of the formation surface 141 has an inclined surface.

  The accommodating portion side surface 143 is typically a surface of the forming surface 141 that extends from the forming surface upper end 149, and usually has an inclination angle of greater than 45 °. The housing portion side surface 143 may be a surface having an inclination angle of more than 90 ° or a surface of 90 °, and an inclination angle of less than 90 °, for example, 70 ° or more and less than 90 °, preferably 80. It may be a surface having an angle of not less than 90 ° and less than 90 °, but is preferably a surface having an inclination angle of 90 ° or less than 90 °. When the accommodation portion side surface 143 is a surface of less than 90 °, the accommodation portion side surface 143 and the accommodation portion bottom inclined surface 142 are integrated to form an inclined surface extending from the peripheral edge of the forming surface 141 to the deepest portion 148.

  In the first embodiment, the deepest part 148 of the forming surface 141 is formed so as to fit in a circle C having a diameter D described later in a plan view of the cell treatment container 100. The deepest portion 148 may be a surface, a point, a line, or 2 of these as long as it is formed so as to fit within the range of the circle C having the diameter D. The shape may be a combination of two or more. In the present invention, that the deepest part 148 is within the range of the circle C having the diameter D in the plan view of the cell treatment container 100 is the same as the circle C having the diameter D in the plan view of the deepest part 148. Or, it indicates that the shape is a shape included in a circle C, and the diameter of the smallest circumscribing circle that includes the shape of the deepest portion 148 in the plan view is D or less. Is synonymous with that. For example, when the deepest portion 148 has a shape of a point, a circle having a diameter of 0.1 μm, a linear shape having a length of 2 μm, etc. in a plan view, the deepest portion 148 having such a shape has a diameter of 2 μm, or It can be said that it is formed so as to fit within the range of a circle C having a larger diameter.

  At least one of the deepest portion 148 of the forming surface 141 and a portion of the accommodation portion bottom inclined surface 142 adjacent to the deepest portion 148 forms a cell holding region 146 that holds cells.

  The cell holding area 146 accommodates the cells 10 and the cell treatment liquid 20 in the accommodation space 150 in a state where the cell treatment container 100 is placed on a horizontal surface under gravity, and the cells 10 move vertically downward due to gravity. This is a region that retains the cell 10 when it finally stops. Under the weight, the cells 10 held in the cell holding region 146 can return to the top of the deepest part 148 by the accommodation part bottom inclined surface 142 adjacent to the deepest part 148 even if the cells 10 are moved horizontally by vibration.

  FIG. 1H shows an example (the same as the embodiment shown in Examples 1A to 1G) of the cell holding region 146 in which the deepest part 148 is a point. In this example, since the deepest portion 148 is a point, it is within the range of a circle C having a diameter D described later in a plan view of the cell treatment container 100. When the cells 10 are added to the accommodation space 150 (same) together with the cell treatment liquid 20 (not shown in FIG. 1H), the cells 10 are propagated on the accommodation part bottom inclined surface 142 which is the inclined surface portion of the forming surface 141 by gravity. To reach the cell holding region 146, the periphery of the cell 10 is surrounded by the portion of the accommodation portion bottom inclined surface 142 adjacent to the deepest portion 148, and the cell 10 is supported. At this time, the position of the cell 10 is determined at one point. Therefore, it is possible to prevent the cells 10 from disappearing from the observation field of view by the microscope due to the unintended horizontal movement during the cell treatment.

  FIG. 1I shows an example of the cell holding region 146 whose surface is the deepest part 148. In this example, the deepest part 148 has a horizontal expansion, but is formed so as to fit within a circle C having a diameter D described later. The outer circumference of the deepest part 148 is surrounded by the housing part bottom inclined surface 142. Even if the size of the cell 10 is smaller than the width of the planar deepest part 148 and can move horizontally within the deepest part 148 as shown in FIG. Since the movement in the horizontal direction is limited, the range in which the cell 10 can move in the horizontal direction is limited to the range of the deepest portion 148 within the range of the circle C having the diameter D. Although not shown, when the area of the planar or linear deepest portion 148 is relatively small with respect to the cell 10 to be treated and is covered with the cell 10, the cell is similar to the example shown in FIG. 1H. The periphery of 10 is surrounded by the portion of the accommodation portion bottom inclined surface 142 adjacent to the deepest portion 148, the cells 10 are supported, and the position of the cells 10 is determined at one point.

  In the first embodiment, the diameter D of the circle C is 3 mm. When the diameter D of the circle C is 3 mm, the cells accommodated in the accommodation space 150 of the accommodation unit 130 are present within a narrow circle having a diameter of 3 mm at the maximum in a plan view. Since the cells can be confirmed by observing a predetermined position including the deepest part 148 at an observation magnification (for example, 30 to 50 times) that is easy to work, the cells 10 disappear from the observation field under the microscope during the cell treatment. It is possible to avoid the above, and it is not necessary to confirm the cell position in advance by microscopic observation at a low magnification (for example, 20 times), which simplifies the work. When the diameter D of the circle C is 3 mm, the diameter d of the smallest circumscribing circle that includes the shape of the deepest part 148 of the cell treatment container 100 in plan view is 3 mm or less. When a catheter having an outer diameter equal to or larger than the diameter d is used, if the catheter tip is moved so as to approach the cell holding region 146, the catheter tip is positioned at a position covering the entire deepest part 148. The cell (embryo) 10 can be easily handled with a catheter. That is, if a catheter having an outer diameter of d or more is used as a catheter for handling embryos, stable work can be performed by moving the catheter tip to the same position each time. The outer diameter of the catheter used to handle the embryo is usually 3 mm or less. In the cell treatment container 100, since the deepest part 148 is set within the range of the circle C having a diameter of 3 mm, that is, the diameter d is 3 mm or less, it has a general dimension (the outer diameter is 3 mm or less). Among the catheters for handling the embryo, a catheter having an outer diameter of the catheter tip equal to or larger than d and capable of covering the entire deepest part 148 can be appropriately selected and used.

  In a preferred embodiment of this Embodiment 1, the diameter D of the circle C is more preferably 2.5 mm, more preferably 2 mm, more preferably 1.8 mm, more preferably 1.6 mm, more preferably 1.5 mm. Preferably 1.4 mm, more preferably 1.2 mm, more preferably 1 mm, more preferably 970 μm, more preferably 900 μm, more preferably 700 μm, more preferably 500 μm, more preferably 300 μm, more preferably 200 μm, more preferably Is 100 μm, more preferably 50 μm, more preferably 10 μm, particularly preferably 5 μm, most preferably 2 μm. As the diameter D of the circle C is smaller, the position of the cell 10 is set within a narrower range, so that it is possible to reduce the possibility that the cell 10 will disappear during observation with a high-power microscope. Moreover, in the cell treatment container 100, the smaller the diameter D of the circle C is, the more suitable it is to use a catheter having a small outer diameter and a smaller burden on the patient. In particular, the cell treatment container 100 having a diameter D of 1 mm, preferably 970 μm, and more preferably a smaller value uses a catheter having a small outer diameter such as 3 Fr (1 mm) and 4 Fr (1.33 mm). It is suitable and suitable for handling the cells 10.

In another preferred embodiment of Embodiment 1, the deepest portion 148 of the forming surface 141 has an area of less than 0.75 mm ² , preferably 0.74 mm ² or less, more preferably 0 in plan view of the cell treatment container 100. .7Mm ² or less, more preferably 0.5 mm ² or less, more preferably formed to be 0.3 mm ² or less. In this case, since the cells accommodated in the accommodation space 150 of the accommodation unit 130 are present in the area having the small area in plan view, the cells are accommodated in the area having the small observation area at the observation magnification that is easy for the operator to work from the beginning. Since it is possible to confirm the cells by observing, the cells 10 can be prevented from disappearing from the observation field under the microscope during the cell treatment. In the another preferred embodiment, the diameter D is not particularly limited as long as it is 3 mm or less, but it is preferably the same value as the preferred value of the diameter D in the preferred embodiment.

  Further, in the present embodiment, since the accommodation portion bottom inclined surface 142 is formed from the peripheral edge of the forming surface 141 to the deepest portion 148, the cells 10 are unlikely to settle midway. Then, as described above, the cells 10 are held in the cell holding region 146, and therefore, observation with a microscope is easy. Further, as shown in FIG. 1E, when the cells 10 are sucked by the catheter 30, the positions of the cells 10 are fixed, so that the cells 10 can be sucked easily.

  A portion of the inclined surface (housing portion bottom inclined surface 142) adjacent to the deepest portion 148 included in the cell holding region 146 is a portion of the inclined surface that plays a role of limiting the horizontal movement of the cells 10. It is determined according to the size of the cell 10 and the like. For example, in a plan view in a state where the cell treatment container 100 is placed on a flat horizontal surface, it is a portion within a range of 1 mm from the contact point with the deepest portion 148 in the inclined surface (storing portion bottom inclined surface 142).

  In the present embodiment, the slope of the inclined surface in the forming surface 141, such as the accommodation portion bottom inclined surface 142, may be constant from the peripheral edge of the forming surface 141 to the deepest portion 148, or may be continuous or stepwise. However, it is preferably constant at least in the cell holding region 146. On an inclined surface having a constant gradient, the shape of the plane along the vertical direction on the cross section is linear. When the change in the gradient on the inclined surface of the cell holding region 146 is small, the cells 10 can be satisfactorily observed with little light scattering during observation with a microscope. More preferably, as shown in the drawing, the entire housing bottom inclined surface 142 has a constant slope from the peripheral edge of the housing bottom inclined surface 142 to the deepest portion 148. Note that the constant slope of the inclined surface 142 does not necessarily mean that the slope is constant, but also includes the case where the slope is substantially constant. The phrase that the gradient is substantially constant means, for example, that the change in the gradient is so small that the effect at the time of observation with the above-described microscope is exhibited.

The inclination angle θ ₁ of the inclined surface portion such as the accommodation portion bottom inclined surface 142 included in the forming surface 141 is preferably 1 ° to 45 °, more preferably 2 ° to 25 °, at least in the cell holding region 146. , Particularly preferably 5 ° to 10 °. When the inclination angle θ ₁ is within this range, reflection and scattering on the inclined surface are less likely to occur when the cell 10 on the cell holding region 146 is observed by transmission with a microscope, and a clear observation image can be obtained. More preferably, the inclination angle θ ₁ is within the above range for the entire inclined surface included in the housing portion inclined surface 142. In this case, it is easy to move the cell 10 to the cell holding region 146 using gravity as a power source.

  If the surface roughness of the inclined surface is a large value, when the image that has been observed by transmission with a microscope is subjected to the contour extraction processing, a clear contour may not be obtained due to unevenness on the inclined surface. Therefore, the value is preferably as small as possible. Specifically, the maximum height Ry (only the reference length is extracted from the roughness curve in the direction of the average line, and the distance between the peak line and the valley bottom line in this extracted portion) is less than 4.0 μm. Is preferred, less than 1.0 μm is more preferred, and less than 0.5 μm is particularly preferred. The maximum height Ry complies with JIS B0601-1994. When the surface roughness of the inclined surface is within this range, light scattering can be sufficiently suppressed and a clear contour can be obtained during transmission observation. The surface roughness of the inclined surface can be reduced by increasing the processing accuracy of the mold, for example, by performing a polishing treatment when manufacturing the mold of the cell treatment container.

  When the deepest part is a surface, the surface roughness of the deepest part is preferably in the same range as above.

More preferably, at least in the cell holding region 146, the angle formed by the facing portions of the inclined surfaces included in the forming surface 141 (angle corresponding to θ ₂ below) is larger than 90 °, and more preferably 150 ° or more. And more preferably 160 ° or more. The upper limit is not particularly limited as long as it is less than 180 °. Reference is now made to FIG. 1G to describe the corner. FIG. 1G is an enlarged view of the vicinity of the cell holding region 146. The facing portions of the accommodation portion bottom inclined surface 142 are a pair of portions A and B that are included in the accommodation portion bottom inclined surface 142 and have the same vertical position, and are the normal lines of the accommodation portion bottom inclined surface 142 that pass through the portion A. L _A and a normal line L _{B of the} accommodation portion bottom inclined surface 142 passing through the portion B indicate a pair of portions A and B passing on one plane parallel to the vertical direction. Then, the angle which the portion forms, in the said plane, from the portion A normal L _A, the part B pulls the normal L _B, when the intersection and P, between A and P and B It refers to the outside angle θ ₂ . When θ ₂ is larger than 90 ° at least in the cell holding region 146 of the forming surface 141, the stored cells 10 can be easily taken out from the cell holding region 146, and the catheter 30 was used as shown in FIG. 1E. The possibility of damaging the cell 10 when handling the cell 10 is low.

More preferably, as shown in the figure, θ ₂ satisfies the above condition not only in the cell holding region 146 of the forming surface 141 but also in the vicinity thereof. Of the forming surface 141, the vicinity of the cell holding region 146 is a range from the deepest portion 148 to preferably 10 mm in plan view of the forming surface 141 in a state where the cell treatment container 100 is placed on a flat horizontal surface, It is a portion on the forming surface 141 that is more preferably up to 8 mm, and further preferably up to 3 mm. Since the outer diameter of the catheter 30 used for handling the embryo for transplantation is usually 1 mm to 3 mm, if the portion near the cell holding region 146 on the forming surface 141 satisfies the condition of θ ₂ , the catheter 30 is used. The handling of the used cells 10 becomes easier.

The cell treatment container 100 preferably has a formation surface 141 including a housing portion bottom surface 400 (the housing portion bottom surface inclined surface 142 and the deepest portion 148) and a housing portion side surface 143, as in the first embodiment shown in FIG. 1. In the first embodiment, more preferably, the angle θ formed between the housing bottom surface 400 and the housing side surface 143 in the housing space 150 at the bottom surface side crossing portion 147 which is the portion where the housing bottom surface 400 and the housing side surface 143 intersect. ₃ is larger than 90 °, more preferably 95 ° or more, and the upper limit is not particularly limited, but is 135 ° or less, for example. theta ₃ is accommodated bottom face side of the normal line _{L C} and an angle made (normal _{L D} of the bottom surface side intersection 147 of the normal line _{L D} and the accommodating portion side 143 of the bottom surface side intersection 147 of the bottom 400 It coincides with a half line extending from the intersecting portion 147 to the side opposite to the accommodation space 150 and an angle formed by a half line extending from the bottom surface side intersecting portion 147 to the accommodation space 150 side of the normal line L _C (FIG. 1B). . When the angle θ ₃ is in this range, even if the cell 10 enters the bottom surface side crossing portion 147, the cell 10 can be taken out relatively easily, and the cell 10 can be taken out without damaging the cell 10.

  The height from the deepest part 148 to the upper end 149 of the forming surface 141 is preferably 2 mm or more and 12 mm or less (2 mm to 12 mm or 2 to 12 mm), preferably 3 mm or more, preferably 4 mm or more, preferably 5 mm or more, It is preferably 11 mm or less, preferably 9 mm or less, preferably 8 mm or less, more preferably 3 mm or more and 11 mm or less (3 mm to 11 mm or 3 to 11 mm), and further preferably 4 mm or more and 9 mm or less (4 mm to 9 mm or 4 to 9 mm). , And more preferably 5 mm or more and 8 mm or less (5 mm to 8 mm or 5 to 8 mm). Here, the height from the deepest part 148 of the forming surface 141 to the upper end 149 refers to the distance from the deepest part 148 of the forming surface 141 to the upper end 149 of the forming surface 141 in the up-down direction of the accommodating portion 130. When an arbitrary point on the upper end 149 of the forming surface 141 does not lie on a plane perpendicular to the vertical direction, the smallest height of the forming surface 141, more preferably all heights, is set. The value may be within the above numerical range. When the height of the forming surface 141 is within the numerical range, when the cell treatment liquid 20 is stored in the storage space 150, the depth from the liquid surface to the cell holding region 146 becomes a sufficient depth. This is advantageous because the cells 10 in the cell treatment liquid 20 positioned in the region 146 can be easily handled by the catheter 30 or the like. In particular, it is advantageous when a catheter with a flexible tip and a wide width is used, such as a silicone rubber catheter used for handling embryos for transplantation. Further, when the height of the forming surface 141 is 2 mm or more, the cells 10 such as transplanted embryos are sufficiently immersed, and the amount of the liquid for suppressing the change of the component due to the volatilization of the cell treatment liquid 20 is left for a short time and the like. Is possible, which is preferable. When the height is 4 mm or more, the depth from the liquid surface to the cell holding region 146 becomes a sufficient depth, so that the tip of the catheter is easily immersed in the cell treatment liquid 20 at the time of catheter work to suck bubbles. This is preferable because the risk of mistakes can be reduced. When the height is 5 mm or more, the risk of liquid leakage can be reduced when the cell treatment liquid 20 has a liquid depth of about 3 mm, which is preferable. If the height is 12 mm or less, the working angle of the catheter, glass capillary, or the like during the microscope observation becomes a relatively obtuse angle, so that the risk of contacting the lens of the microscope which may occur in the case of an acute angle, or the catheter This is preferable because it is possible to reduce the risk of causing the tip of the glass capillary or the like to come into contact with the accommodating portion peripheral wall portion 140 including the forming surface 141 and cause damage or damage. When the height is 9 mm or less, the risk can be further reduced, which is preferable.

A more preferable aspect of the present invention is, as in the present embodiment, when the cell treatment container 100 is placed on the horizontal surface H, one point included in the deepest part 148 on the forming surface 141 and the forming surface upper end 149. This is a mode in which the smallest angle of the acute angle θ ₄ formed by the imaginary straight line L ₂ connecting one point included in 1 with the horizontal plane H is 45 ° or less (see FIG. 1J). As described above, the upper end 149 of the forming surface is the upper edge of the peripheral edge of the forming surface 141 in plan view, and the deepest portion 148 is not limited to a dot shape, and may be a surface shape or a linear shape. Therefore, θ ₄ can take various values in one cell treatment container 100, but in the present embodiment, the smallest angle of θ ₄ may be within the above range. When the smallest angle of θ ₄ is in the above range, the following effects can be achieved. When sucking cells such as a fertilized egg held in the cell holding region 146 including the deepest part 148 with a catheter, it is common to suck the cells up to a position of about 4 mm from the tip of the catheter. Within the above range where θ ₄ is the smallest, the operation of bringing the catheter tip closer to the cells held in the cell holding area 146 and sucking to a position 4 mm from the catheter tip is focused on the cells held in the cell holding area 146. When observed with a stereoscopic microscope at a magnification of 20 in this state, the angle between the length direction of the catheter and the horizontal plane H can be set to 45 ° or less, and a portion 4 mm from the cell holding region 146 and the catheter tip. Since the difference in the vertical position between and is small, cells can be visually recognized in the observation visual field of the microscope. More preferably, the smallest angle of θ ₄ is 40 ° or less, further preferably θ ₄ is 45 ° or less at any part, and most preferably 40 ° or less.

In the present embodiment, the upper edge of the outer peripheral wall portion inner peripheral surface 121 is the outer peripheral wall portion inner peripheral surface upper end 123. When the cell treatment container 100 is placed on the horizontal plane H, a virtual straight line L ₃ connecting one point included in the deepest part 148 of the forming surface 141 and one point included in the upper end 123 of the outer peripheral wall inner peripheral surface is formed. The smallest angle of acute angles θ ₅ formed with the horizontal plane H is preferably 45 ° or less, more preferably 40 ° or less, and further preferably θ ₅ is 45 ° or less in any part, and most preferably 40 ° or less. is there.

Further, θ ₄ and θ ₅ are preferably 81 ° or less at maximum. At this time, as confirmed in Experiment 5, the catheter does not interfere with the objective lens, the accommodating part peripheral wall part 140, and the outer peripheral wall part 120 during observation with a stereoscopic microscope having a magnification of 20 times.

Further, when the height from the deepest part 148 of the formation surface 141 to the upper end 149 is 4 mm or less, the smallest angle of θ ₄ is preferably 25 ° or more. When a catheter having a tip width of 4 mm or more is used, if the angle is less than 25 °, the tip of the catheter is placed in a cell holding region so that the angle formed by the length direction of the catheter and the horizontal plane H is less than 25 °. Although it can be brought close to 148, there is a risk that air bubbles easily enter the catheter at that time and cells are killed by an operation error. This problem is particularly remarkable when a catheter having a tip width of about 1 mm to 2 mm is used. It is preferable that the smallest angle of θ ₄ is 25 ° or more because the risk can be avoided.

  The shape of the figure formed by the opening of the accommodation section 130 in plan view, that is, the figure surrounded by the inner peripheral side contour of the accommodation section upper end 145 is not particularly limited, and the first embodiment and the second, fourth, and fifth embodiments described later. Such as a circular shape (including a circle and an ellipse), a rectangle (including a square and a rectangle) like Embodiment 3 to be described later, other polygons, and a variation 1 and 2 of Embodiment 1 to be described later. The shape may be a combination of a plurality of different shapes.

  In a plan view, the graphic may have a shape that is long in one direction (for example, an ellipse, a flat circle, a rectangle) as in the third and fourth embodiments and the first modification of the first embodiment. 5, N-fold symmetry (N is an integer of 3 or more, preferably an integer of 4 or more, more preferably an integer of 4 to 8) as in the second modification of the first embodiment (for example, a circle, a regular polygon). May be Here, the “N-fold symmetric shape” does not need to have strict symmetry, and includes a case of a substantially N-fold symmetric shape.

  When the figure formed by the opening of the housing section 130 in a plan view has a shape that is long in one direction as in the third and fourth embodiments, the orientation of the opening of the housing section 130 can be recognized, so that the operator can perform work. There is an advantage that mistakes can be reduced. Further, since the opening of the housing portion 130 provides an expanded working area expanded in the longitudinal direction defined below, the device is arranged when the cells housed in the housing space 150 are handled by a device such as a catheter. There is an advantage that the workable area is expanded and the workability is improved. In this embodiment, the one direction is defined as a “major axis direction”, and the distance between a pair of points on the peripheral edge of the figure that are opposed to each other along the major axis direction with the center of gravity of the figure therebetween is defined as a “long axis direction”. The width in the axial direction ", the direction orthogonal to the major axis direction in a plan view is defined as the" minor axis direction ", and the most of the distances between a pair of points on the peripheral edge of the figure facing each other along the minor axis direction. The large distance is defined as the “width in the minor axis direction”. The width in the minor axis direction can be, for example, 3 mm or more and 15 mm or less (3 mm to 15 mm or 3 to 15 mm), and the width in the major axis direction can be, for example, 3.6 mm or more and 30 mm or less (3.6 mm to 30 mm or 3.6 to 30 mm). Can be When the width in the short axis direction and the width in the long axis direction of the opening of the accommodating portion 130 are within the above range, it is particularly easy to handle cells using a catheter having a wide tip. The ratio of the width in the minor axis direction to the width in the major axis direction is preferably 1: 1.2 to 1: 3, and more preferably 1: 1.5 to 1: 2. When the width in the short axis direction and the width in the long axis direction are in this range, it is possible to more clearly recognize the orientation of the opening of the housing portion 130.

  When the figure formed by the opening of the housing section 130 in plan view has a N-fold symmetry shape, the distance between a pair of points on the peripheral edge of the figure that linearly face each other with the center of gravity of the figure in between. When the minimum value of is the “minimum width”, the minimum width is preferably 3 mm to 30 mm, and more preferably 3.6 mm to 15 mm.

  Another preferable form of the figure formed by the opening of the housing section 130 in plan view is the first portions 261 and 271 as in the first modified example 1 of the first embodiment shown in FIG. 26 and the second modified example of the first embodiment shown in FIG. , A form in which one or more second portions 262, 272 (272-1, 272-2, 272-3, 272-4) extending in at least one direction from the first portions 261, 271 are combined with each other. Is. In these forms, since the storage space 150 is expanded to the second portions 262 and 272, when the cells stored in the storage space 150 are handled by a device such as a catheter, a region where the device can be arranged is widened and workability is improved. Is improved.

  Further, in the plan view of the cell treatment container 100, the cell holding region 146 of the forming surface 141 has a center of gravity that is relative to the figure formed by the opening of the container 130, that is, the figure surrounded by the contour of the upper end 145 of the container. The cell holding region 146 may be located at a position where the cell holding region 146 overlaps with the center of gravity as in Embodiment 1 and Embodiments 3, 4 and 5 described later, or as in Embodiment 2 described below. The holding region 146 may be located at a position where it does not overlap with the center of gravity, that is, at a position where the cell holding region 146 is eccentric with respect to the figure formed by the opening of the container 130.

  Further, the position of the container 130 on the container bottom 110 is not particularly limited. When only one accommodation section 130 is present, in a plan view of the cell treatment container 100, a shape formed by the inner peripheral side contour of the opening of the cell treatment container 100 (the inner peripheral side contour of the upper end of the outer peripheral wall 120) is formed. The accommodating portion 130 is preferably formed at a position overlapping the center of gravity. More preferably, in the plan view of the cell treatment container 100, the center of gravity of the figure formed by the contour of the inner peripheral side of the opening of the housing portion 130 and the center of gravity of the figure formed by the contour of the inner peripheral side of the opening of the cell treatment container 100 are determined. In the same position. Here, “identical” includes “substantially identical”.

  The capacity of the accommodation space 150 formed by the accommodation section 130 is 200 μl or more and 1000 μl or less (200 μl to 1000 μl or 200 to 1000 μl), preferably 250 μl or more, preferably 300 μl or more, preferably 800 μl or less, preferably 500 μl or less, It is preferably 400 μl or less, more preferably 200 μl or more and 800 μl or less (200 μl to 800 μl or 200 to 800 μl), particularly preferably 250 μl or more and 500 μl or less (250 μl to 500 μl or 250 to 500 μl), and most preferably 300 μl or more. It is 400 μl or less (300 μl to 400 μl or 300 to 400 μl). In the present embodiment, the “capacity of the accommodation space” is the lowest of the formation surface upper ends 149 that are the upper ends of the formation surfaces 141 that form the accommodation space 150 when the cell treatment container is placed on a flat horizontal surface. Indicates the volume of the space surrounded by the formation surface 141 and an imaginary plane that passes through the portion located at and is parallel to the horizontal plane.

  If the volume of the accommodation space 150 is 200 μl or more, a sufficient amount of the cell treatment liquid 20 can be accommodated in the accommodation space 150, so that the disappearance and composition change of the cell treatment liquid 20 due to volatilization can be sufficiently suppressed. You can Therefore, the cell treatment container 100 of the present embodiment can be suitably used for applications in which the surface of the cell treatment liquid cannot be covered with oil, such as the treatment of the embryo for transplantation immediately before the transplantation with the transplant liquid. On the other hand, since the capacity of the accommodation space 150 is 1000 μl or less, the problem that the excess of the cell treatment solution that accommodates a large amount of the cell treatment solution 20 in the accommodation space 150 is likely to spill is unlikely to occur. Although some of the cell treatment liquids 20 are expensive, the treatment cost can be suppressed by setting the volume of the accommodation space 150 to 1000 μl or less. Moreover, the volume of 200 μl or more and 1000 μl or less is a volume particularly suitable for the purpose of treating one transplant embryo with a transplant liquid immediately before transplantation to a mother.

  Further, in order to make the volume of the accommodation space 150 larger than 1000 μl, the height of the accommodation part peripheral wall part 140 from the container lower surface 112 or the deepest part 148 to the accommodation part upper end 145 is increased, or the flat surface of the accommodation space 150. It is necessary to increase the visual width. However, if the height of the accommodating part peripheral wall part 140 is increased, when the cells accommodated in the accommodating space 150 are operated by the catheter, the accommodating part peripheral wall part 140 hinders the catheter operation, resulting in a complicated operation. is there. Further, if the width of the accommodation space 150 in plan view is increased, it is possible to increase the capacity by increasing the height of the accommodating portion peripheral wall portion 140 that hinders the operation, but on the other hand, the surface area of the treatment liquid to be accommodated. Therefore, there is a problem that the volatilization of the liquid is promoted, and when the amount of the processing liquid used is reduced, the volatilization is promoted, and it is difficult to adjust the amount of the processing liquid to be small. Further, when the capacity of the accommodation space 150 increases, the amount of the treatment liquid to be accommodated also increases, and the range in which a device such as a catheter is immersed in the treatment liquid increases, so that there is a problem that the probability of contamination by microorganisms increases. . By setting the volume of the accommodation space 150 to 1000 μl or less, the above problem can be solved.

  When an embryo for transplant is used as a cell and an embryo for transplant is used as a cell treatment liquid, as shown in FIG. 1D, the embryo for transplant 10 and the embryo 20 are housed in a housing space 150, and necessary. It is covered with an outer lid 170 according to the requirements and placed in an incubator in which the temperature, humidity, gas concentration, etc. are controlled. In general cell culture, in order to prevent volatilization of the cell treatment liquid 20, oil is filled so as to entirely cover the bottom surface 111 of the container and the container 130 in which the cells 10 and the cell treatment liquid 20 are contained. (Not shown). However, when treating embryos for transplantation, coating with oil is not possible. In the cell treatment container 100 of the present embodiment, the volume of the accommodation space 150 is 200 μl or more, which is a relatively large volume, and therefore, the volatilization of the cell treatment liquid 20 is relatively small even when oil is not used, which is advantageous. .

Hereinafter, another embodiment shown in the drawings will be described. In the drawings of this specification, configurations having the same function are denoted by the same reference numerals regardless of the size and shape of each configuration, and description thereof is omitted except for special differences from the first embodiment.
<Embodiment 2 (FIGS. 2A and 2B)>
The second embodiment is a form of the first embodiment, and the cell holding region 146 of the forming surface 141 is a figure formed by the opening of the container 130 in a plan view of the cell treatment container 100, that is, the upper end 145 of the container. It is characterized in that it is formed at a position that does not overlap with the center of gravity of the figure surrounded by the inner contour.

  According to the second embodiment, in a plan view, the cell holding region 146 is located at an eccentric position in the accommodation space 150, so that a relatively large space margin is provided on the side of the accommodation space 150 where the cell holding region 146 is not formed. You can Therefore, when the cells supported by the cell holding region 146 are handled by a catheter or pipette, the catheter or pipette can be inserted through the space, which is advantageous because the operation is facilitated.

  Moreover, since the position of the cell holding region 146 is eccentric, it becomes easy for the user to grasp the orientation of the cell treatment container 100.

The features such as the structure, shape, dimensions, and angles of the other parts in the second embodiment are the same as those described above in the first embodiment.
<Embodiment 3 (FIGS. 3A and 3B)>
The third embodiment is one form of the first embodiment, and the shape formed by the opening of the housing portion 130 in plan view, that is, the shape surrounded by the inner peripheral side contour of the housing upper end 145 is rectangular. Characterize. In the third embodiment, the figure surrounded by the bottom surface side crossing portion 147, which is the upper end of the housing bottom surface 400, is also rectangular, and the housing bottom inclined surface 142 is an inverted quadrangular pyramid surface.

  In the third embodiment, the ratio between the width in the short axis direction and the width in the long axis direction of the rectangle formed by the opening of the housing portion 130 in plan view is not particularly limited, but is preferably 1: 1.2 to 1: 3.

  When the figure formed in the plan view of the opening of the container 130 is rectangular, the user can easily grasp the orientation of the cell treatment container 100. In addition, a relatively large space can be provided in the accommodation space 150 along the long axis direction. Therefore, when the cells supported by the cell holding area 146 are handled by the catheter, the catheter can be inserted through the space, which is advantageous because the operation is facilitated.

The features such as the structure, shape, dimensions, and angles of the other parts in the third embodiment are the same as those described above in the first embodiment.
<Embodiment 4 (FIGS. 4A and 4B)>
The fourth embodiment is one form of the first embodiment, and the shape formed by the opening of the housing portion 130 in plan view, that is, the shape surrounded by the inner peripheral side contour of the housing portion upper end 145 is a flat circle. Is characterized by. In the fourth embodiment, the figure surrounded by the bottom surface side crossing portion 147, which is the upper end of the housing section bottom surface 400, is also a flat circle of the same shape.

  In the fourth embodiment, the ratio of the width in the minor axis direction to the width in the major axis direction of the flat circle formed by the opening of the housing portion 130 in plan view is not particularly limited, but is preferably 1: 1.2 to 1: 3. .

  When the figure formed in the plan view of the opening of the container 130 is a flat circle, the user can easily grasp the orientation of the cell treatment container 100. In addition, a relatively large space can be provided in the accommodation space 150 along the long axis direction. Therefore, when the cells supported by the cell holding area 146 are handled by the catheter, the catheter can be inserted through the space, which is advantageous because the operation is facilitated.

The features such as the structure, shape, dimensions, and angles of the other portions in the fourth embodiment are the same as those described above in the first embodiment.
<Embodiment 5 (FIG. 5)>
The fifth embodiment is a form of the first embodiment, in which the forming surface 141 has a deepest portion 148 and a housing portion bottom inclined surface 142 extending from the forming surface upper end 149 to the deepest portion 148, and It is characterized in that the inclination angle of the bottom inclined surface 142 is 45 ° or less at any part.

  In the fifth embodiment, the bulge of the accommodation space 150 in the direction perpendicular to the vertical direction is small. Therefore, as compared with the case where the accommodation section bottom inclined surface 142 includes a portion whose inclination angle exceeds 45 °, accommodation when the height from the deepest portion 148 to the upper end 149 of the forming surface 141 is set to a predetermined value. The capacity of the space 150 can be reduced. Although some of the cell treatment solutions are very expensive, according to the fifth embodiment, the cell treatment solution accommodated in the accommodating space 150 has a sufficient depth for operation of a catheter or the like. It is advantageous in that the amount can be reduced.

The features such as the structure, shape, dimensions, and angles of the other parts in the fifth embodiment are the same as those described above in the first embodiment.
<Embodiment 6 (FIGS. 6A to 6G)>
The cell treatment container of the present invention may further be provided with one or more container-shaped parts in which a container space for containing cells and / or liquid is formed at the container bottom. Here, the “container-shaped portion” is a storage space (container-shaped portion storage space) for storing cells and / or liquid, which can be provided in a part of the cell treatment container of the present invention separately from the storage portion. Is a structure formed. The container-shaped portion accommodation space may have any shape as long as it is a space capable of accommodating cells and / or liquid for the purpose of culturing cells, washing cells, and the like. The container-shaped portion may have any shape as long as the container-shaped portion accommodation space is formed, but generally has a bottomed shape that opens upward. The one or more container-shaped portions can be used for the purpose of culturing cells, washing cells, washing catheters, and the like. Embodiment 6 is shown in FIGS. 6A-6G as an example of this aspect of the invention.

  The cell treatment container 100 according to the sixth embodiment is different from the cell treatment container 100 according to the first embodiment in that one culture vessel-shaped portion 610 and three washing vessel-shaped portions 620 are provided on the vessel bottom 110. . In the sixth embodiment, the culture container-shaped portion 610 and the cleaning container-shaped portion 620 are formed so as to project from the container bottom 110 into the outer peripheral space 160.

  By providing one cell treatment container 100 with one or more container-shaped portions 610 and 620 in addition to the container 130, the following advantageous effects can be realized. For example, in order to culture the embryo for transplantation, a step 1 of culturing the embryo after fertilization in a culture medium, and a step of washing the culture medium or the oil used for coating the culture medium from the embryo after the culture In some cases, step 2 and step 3 of immersing the washed embryo in a transplant liquid and treating the embryo are performed. If these steps are performed in different containers, there is a risk of mix-ups when transferring embryos between containers, and management is required to prevent mix-ups, which makes the operation extremely complicated and time-consuming, and It is difficult to perform transplantation quickly. If the cell treatment container 100 of the sixth embodiment is used, step 1 can be performed in the culture container part 610, step 2 can be performed in the cleaning container part 620, and step 3 can be performed in the container part 130. This eliminates the need to transfer the embryos between the plants, which simplifies the work and reduces the risk of mistaking and damaging the embryos.

  The culture container-shaped portion 610 includes a culture container-shaped portion peripheral wall portion 611 standing upright from the container bottom 110, and forms a culture container-shaped portion accommodation space 612 as a space surrounded by the culture container-shaped portion peripheral wall portion 611. ing. The culture container-shaped portion 610 has a culture container-shaped portion bottom surface 613 forming a bottom of the culture container-shaped portion accommodation space 612, and a lower end of the culture container-shaped portion bottom surface 613. And a culture container-shaped portion side surface 614 whose upper end surrounds the opening of the culture container-shaped portion accommodation space 612. One or a plurality of microwells 615 for positioning the cells 630 are formed on the bottom surface 613 of the culture vessel.

In the culture container-shaped portion 610, the capacity of the culture container-shaped portion accommodation space 612 is not particularly limited, and may be, for example, 10 μl to 1000 μl. The definition of the capacity of the culture container-shaped portion accommodation space 612 is the same as the definition of the capacity of the accommodation portion accommodation space 150. In the state where the cell treatment container 100 is placed on a flat horizontal surface, the culture container-shaped portion side surface is formed. A volume of a space surrounded by an imaginary plane that passes through the lowermost portion of the upper end of 614 and is parallel to the horizontal plane, a culture container-shaped portion bottom surface 613, and a culture container-shaped portion side surface 614. . More preferably, a small volume of a culture solution, for example, 10 μl to 100 μl of a culture solution drop (liquid mass) can be contained in the culture container-shaped portion accommodation space 612, and a culture container shape is formed by a drop of the culture solution of 10 μl to 100 μl The culture container-shaped portion accommodation space 612 is formed so that the entire bottom surface 613 can be covered and the height of the drop is 0.35 mm or more, more preferably 0.5 mm or more. For this purpose, the area of the bottom surface 613 of the vessel portion for culture is preferably 0.75 mm ² or more, more preferably 3 mm ² or more, still more preferably 5 mm ² or more, preferably 20 mm ² or less, more preferably 13 mm. It is ² or less. By setting the area of the bottom surface 613 of the vessel portion for culture to be 0.75 mm ² or more, a plurality of microwells 615 capable of accommodating cells, preferably human fertilized eggs, can be arranged on the bottom surface 613 of the vessel portion for culture. . Further, by setting the area of the bottom surface 613 of the vessel portion for culture to 20 mm ² or less, it is possible to cover the entire bottom surface 613 of the vessel portion for culture with a drop of a small volume of culture solution (for example, 10 μl). It is possible to prevent the drop from moving largely on the bottom surface 613 of the culture vessel.

  As shown in FIG. 6C, the culture container-shaped portion 610 forms a drop-shaped culture solution 640 in a culture container-shaped portion accommodation space 612, and arranges cells 630 in a microwell 615 covered with the culture solution 640. Then, it can be used as a culture container for culturing the cells 630. At this time, as shown in the figure, when the upper end 616 of the culture container-shaped portion peripheral wall portion 611 is closer to the container lower surface 112 than the storage portion upper end 145, the outer peripheral space covers the upper surface of the drop-shaped culture solution 640. Oil 650 can be added to 16, and the cells 630 can be cultured in a state where the oil 650 does not enter the accommodation unit internal space 150 while suppressing the drying of the culture solution 640 by the layer of the oil 650.

  To illustrate the preferred embodiment of microwell 615, the vicinity of one microwell 615 is shown enlarged in FIGS. 6D, 6E, 6F and 6G.

  In one embodiment of the microwell 615 shown in FIG. 6D, the microwell 615 is formed on the bottom surface 613 of the culture vessel and has an upward opening shape. In the microwell 615, a microwell internal space 6153 for accommodating cells is formed. The microwell internal space 6153 is formed by the microwell bottom surface 6151 and the microwell side surface 6152 standing upright from the peripheral edge of the microwell bottom surface 6151. The microwell bottom surface 6151 and the microwell side surface 6152 do not have to be clearly distinguishable surfaces as shown in the figure, and may be surfaces that are smoothly continuous with each other. The bottom surface 6151 of the microwell may be a surface recessed downward as shown in the figure, a surface protruding toward the space 6153 in the microwell, or a flat surface. When the bottom surface 6151 of the microwell is a surface recessed downward, the side surface 6152 of the microwell may not exist, and in this case, the peripheral edge of the bottom surface 6151 of the microwell forms the opening of the microwell 615.

  As a more preferable embodiment of the microwell 615, there is an embodiment in which a concavo-convex structure is formed on the side surface 6152 of the microwell. By forming the concavo-convex structure on the microwell side surface 6152, it is possible to prevent bubbles from remaining in the minute microwell internal space 6153 when the culture solution 640 is stored in the culture container-shaped portion 610, and the work of removing the bubbles. It is possible to reduce the frequency of performing. As the concavo-convex structure that can be formed on the microwell side surface 6152, a structure including two or more line-shaped concave portions and / or two or more line-shaped convex portions, or two or more dot-shaped protrusions and / or 2 A structure including the above dot-shaped depressions can be given.

  As the concavo-convex structure including two or more line-shaped concave portions and / or two or more line-shaped convex portions, a structure in which two or more line-shaped convex portions are continuously formed with a space interposed, or two or more A structure in which the line-shaped concave portions are continuously formed with a space in between is exemplified. In a structure in which two or more line-shaped convex portions are continuously formed with a space interposed, the space portion can be regarded as a concave portion, so that substantially two or more line-shaped concave portions sandwich the space. In addition, it is a structure in which line-shaped convex portions and line-shaped concave portions are alternately formed.

  The embodiment of the microwell 615 shown in FIGS. 6E and 6F is one embodiment in which two or more line-shaped concave portions 6154 and two or more line-shaped convex portions 6155 are alternately formed on the microwell side surface 6152. . Hereinafter, the concave portion 6155 will be described as a space between two adjacent convex portions 6154. Each of the line-shaped convex portion 6154 and the line-shaped concave portion 6155 is preferably formed in the direction from the bottom of the microwell 615 toward the opening. When a virtual straight line passing through the center of gravity of the bottom surface of the microwell 6151 in a plan view and extending along the vertical direction of the cell treatment container 100 is projected on the microwell side surface 6152 as “direction 1”, a line-shaped projection It is preferable that each of the portion 6154 and the line-shaped recess 6155 is formed so as to extend along a direction whose angle to the direction 1 is 30 ° or less.

  The line-shaped convex portion 6154 and the concave portion 6155 may be linear or curved, but are preferably linear because they are easy to mold and have a high effect of removing bubbles.

  It is preferable that two or more line-shaped convex portions 6154 have substantially the same width and height even when they are displaced. Further, it is preferable that all the recesses 6155 corresponding to two or more spaces have substantially the same width and height (depth).

In two or more line-shaped concavo-convex structures, the width of the line-shaped convex portion 6154 (X ₁₁ ) and the width of the line-shaped concave portion 6155 (X ₁₂ ) are respectively equal to the length of the edge portion of the opening of the microwell 615. 1/3140 to 1 / 4.5, preferably 1/314 to 1 / 4.5, more preferably 1/165 to 1/15, particularly preferably 1 / 82.5 to 1 / The range is 15. As specific dimensions, the width (X ₁₁ ) of the linear convex portion 6154 and the width (X ₁₂ ) of the linear concave portion 6155 are each preferably 0.1 μm or more, more preferably 1 μm or more, and further preferably Is 10 μm or more, particularly preferably 15 μm or more, particularly preferably 20 μm or more, preferably 100 μm or less, more preferably 70 μm or less, and further preferably 50 μm or less. Each of the widths (X ₁₁ , X ₁₂ ) is measured by, for example, a three-dimensional measurement laser microscope to measure the widths of the convex portions and the concave portions of the plurality of concavo-convex structures in an arbitrary microwell 615, and the average value of the measured values. Can be determined by calculating respectively. By setting the widths of the convex portion 6154 and the concave portion 6155 to be equal to or more than a certain value, it is possible to avoid the possibility that the processing and molding accuracy is deteriorated. Further, by setting the thickness to be equal to or less than a certain value, it is possible to prevent the uneven structure from becoming larger than the structure of the microwell 615, which makes it difficult to form the shape.

  It is preferable that each of the line-shaped convex portion 6154 and the line-shaped concave portion 6155 is formed so that the cross-sectional shape of a plane perpendicular to the vertical direction of the cell treatment container 100 is a square shape or an arc shape. Each side and corner in the shape may be rounded.

  The height of the line-shaped convex portion 6154 with respect to the line-shaped concave portion 6155, which is a space portion, is preferably 0.1 μm or more, more preferably 1 μm or more, still more preferably 5 μm or more, preferably 100 μm or less, more preferably It is 70 μm or less, more preferably 50 μm or less. Here, the height of the line-shaped convex portion 6154 with respect to the line-shaped concave portion 6155 is the distance between the top surface of the convex portion 6154 and the bottom surface of the concave portion 6155 adjacent to the convex portion 6154. Refers to the maximum value. The ratio of the height to the width of the line-shaped convex portion 6154 is preferably 0.05 or more, more preferably 0.1 or more, further preferably 0.2 or more, particularly preferably 0.3 or more, and preferably It is 1.5 or less, more preferably 1.0 or less. The height of the convex portion 6154 with respect to the concave portion 6155 is determined, for example, by measuring the height of the plurality of convex portions 614 in an arbitrary microwell 615 by a three-dimensional measurement laser microscope and calculating an average value of the measured values. can do. Further, the ratio of the height to the width of the protrusion 6154 can be calculated using the values of the width and height of the protrusion 6154 determined by the means described above.

  In the concavo-convex structure including two or more dot-shaped protrusions and / or two or more dot-shaped depressions that can be formed on the microwell side surface 6152, the shape of each protrusion and the depression is not particularly limited, and may be a cone or a cone. Examples include pedestals, pillars, and random anisotropic irregularities. Pyramidal, frustum-shaped, and columnar protrusions and depressions are preferable in terms of workability.

  The embodiment of the microwell 615 shown in FIG. 6G is an embodiment in which two or more columnar protrusions 6156 are formed on the microwell side surface 6152.

  In the case of a cone-shaped protrusion or a truncated cone-shaped protrusion, a shape that becomes thinner toward the tip of the protrusion is preferable from the viewpoint of workability. In the case of a cone-shaped recess or a truncated cone-shaped recess, the opening width becomes wider toward the opening side of the recess. The following shape is preferable from the viewpoint of workability.

  The width of the dot-shaped protrusions and the opening width of the dot-shaped depressions are preferably 0.1 μm or more, more preferably 1 μm or more, still more preferably 5 μm or more, preferably 50 μm or less, more preferably 25 μm or less, further preferably 10 μm. It is the following. By setting the width of the protrusions and the opening width of the depressions to be equal to or greater than a certain value, it is possible to avoid the possibility that the processing and molding accuracy is deteriorated. Further, by setting the thickness to a certain value or less, it is possible to avoid the difficulty of forming the shape because the recessed portion becomes large with respect to the structure of the microwell. The width of the protrusion refers to the maximum value of the maximum diameter in the figure of the cut surface perpendicular to the axis of the protrusion. The opening width of the depression refers to the maximum diameter in the figure of the opening of the depression.

  The height of the dot-shaped projections and the depth of the dot-shaped depressions are preferably 0.1 μm or more, more preferably 1 μm or more, still more preferably 5 μm or more, preferably 50 μm or less, more preferably 25 μm or less, further preferably It is 10 μm or less. The ratio of the height of the protrusion to the width of the protrusion (height / width) and the ratio of the depth of the depression to the opening width of the depression (depth / opening width) are preferably 0.1 or more, more preferably 0. It is 3 or more, preferably 1.5 or less, and more preferably 1 or less.

  By setting the height of the dot-shaped projections and the depth of the dot-shaped depressions to be equal to or higher than a certain level, it is possible to avoid the possibility of deterioration of the processing and molding accuracy. Further, by setting the thickness to a certain value or less, it is possible to prevent the protrusion and the recess from becoming large with respect to the structure of the microwell and making it difficult to form the shape. In addition, by setting the height ratio to be equal to or less than a certain value, it becomes difficult to perform vertical peeling from the mold or the shape is broken when the side surface depressions or protrusions are formed by injection molding or the like. You can avoid the possibility of getting lost.

  The pitch of the dot-shaped projections and the pitch of the dot-shaped depressions are preferably 0.1 μm or more, more preferably 1 μm or more, further preferably 5 μm or more, preferably 50 μm or less, more preferably 25 μm or less, further preferably 10 μm or less. Is.

  By setting the pitch to be a certain value or more, it is possible to avoid the possibility that the processing and molding accuracy is deteriorated. Further, by setting the thickness to a certain value or less, it is possible to prevent the protrusion and the recess from becoming large with respect to the structure of the microwell and making it difficult to form the shape.

  The pitch of the dot-shaped protrusions is the distance between two adjacent protrusions and indicates the distance between the centers of the protrusions. Here, the center of the protrusion is the center of gravity of the figure at the tip of the protrusion. The pitch of the dot-shaped depressions is the distance between two adjacent depressions and indicates the distance between the centers of the depressions. Here, the center of the depression is the center of gravity of the figure of the opening of the depression. Pitch usually refers to the average pitch.For a certain projection, the average pitch is calculated by calculating the average value from the pitches of all the adjacent projections, and for a certain depression, it is calculated from the pitch of all the adjacent depressions. The average value is calculated.

  Although the dot-shaped projections and the dot-shaped depressions may be mixed, it is preferable from the viewpoint of workability that only the dot-shaped projections or only the dot-shaped depressions are formed, and that only the dot-shaped projections are formed. Is more preferable.

Each microwell 615 preferably has an opening width capable of accommodating cells. The opening width of each microwell 615 refers to the size of the width of a figure formed by the peripheral edge of the microwell 615 when the peripheral edge of the opening is projected onto a virtual plane perpendicular to the vertical direction. Furthermore, the minimum value of the opening width is the minimum value of the distance between a pair of points on the peripheral edge of the figure that face each other with the center of gravity of the figure in between (for example, if the figure is a circle, the length of the diameter is In the case of an ellipse, it means the length of the minor axis). When the minimum opening width of each microwell 615 is larger than the maximum size of cells to be cultured, each microwell 615 can accommodate cells. When culturing a fertilized egg using the culture container 610, it is desirable to culture to the blastocyst stage. Therefore, the minimum opening width of each microwell 615 is the maximum of the blastocyst stage cells. It is desirable that the size is larger than the size. Since the maximum size of cells in the blastocyst stage is usually 100 μm to 280 μm, the minimum opening width of each microwell 615 is usually 100 μm or more, and more preferably 250 μm or more. Further, each microwell 615 preferably has dimensions that allow it to be densely arranged within a small area. The maximum value of the opening width of each microwell 615 is usually set to be smaller than the pitch between adjacent microwells 615. The maximum opening width of each microwell 615 is preferably less than 1000 μm, more preferably less than 700 μm. Here, the maximum value of the opening width of each microwell 615 means that in the figure formed by the periphery of the opening of each microwell 615 when projected on the virtual plane perpendicular to the vertical direction, It refers to the maximum value of the distance between a pair of points on the peripheral edge of the graphic that face each other with the center of gravity of the graphic interposed therebetween. By setting the maximum value of the opening width of each microwell 615 to less than 1000 μm, the pitch between the adjacent microwells 615 can be made small, and a plurality of them can be formed in a small area in the bottom surface 613 of the culture vessel. Since it is possible to condense the microwells 615 of the above, it is possible to culture a plurality of cells at the same time while managing the cells individually. Furthermore, since many cells are included in one field of view of the microscope, many cells can be observed at once can do. Further, by setting the maximum value of the opening width of each microwell 615 to less than 1000 μm, the movement of cells within each microwell 615 is small and observation is easy. Adjacent microwells 615 are preferably arranged at a density of 1 or more, more preferably 4 or more per 1 mm ² .

  When culturing bovine fertilized eggs in the culture container 610, the minimum opening width of each microwell 615 is usually 250 μm or more, preferably 260 μm or more, and more preferably 270 μm or more. The maximum value of the opening width is usually less than 1000 μm, more preferably less than 700 μm. Further, the minimum value of the opening width of each microwell 615 can be defined as X + m (where X represents the maximum diameter of cells). Here, m is preferably 10 μm or more, more preferably 20 μm or more.

  The pitch between adjacent microwells 615 is the distance between the centers of adjacent microwells 615. The center of the microwell 615 is the center of gravity of the figure formed by the peripheral edge of the opening of the microwell 615 when projected onto the virtual plane perpendicular to the vertical direction, and if the peripheral edge of the opening is circular. It is the center of the circle. The pitch between the microwells 615 usually refers to an average pitch, and the average pitch refers to a certain microwell 615 obtained by calculating an average value from the pitches of all the microwells 615 that are adjacent to each other.

  When the periphery of the opening of the microwell 615 is circular, the pitch between the adjacent microwells 615 is X + m + n (where X represents the maximum diameter of the cell, and m is a circle formed by the periphery of the opening of the microwell 615). Of the microwells 615) and n represents the length of the partition between the microwells 615). The partition between the microwells 615 refers to the shortest distance between the peripheral edges of the openings between the adjacent microwells 615. Here, m is usually 100 μm or less, preferably 70 μm or less, more preferably 50 μm or less, and n is usually 600 μm or less, preferably 350 μm or less, more preferably 150 μm or less.

  By arranging the microwells 615 densely with the pitch as described above, many cells can be cultured at the same time while individually managing the cells, and more cells enter one field of the microscope. Can be observed.

  The depth of each microwell 615 is not particularly limited, but if it is too shallow, cells move outside the range of each microwell 615 when the culture vessel is transported or cells divide. Since there is a possibility that the cells may come out, it is appropriately set so that the cells can be surely held in each microwell 615. On the other hand, if it is too deep, it becomes difficult to introduce the culture solution or cells into each microwell 615. Therefore, the value is set appropriately so as not to be too deep while holding the cells in each microwell 615. For example, the upper limit of the depth can be three times or less than the average value of the maximum value and the minimum value of the opening width of each microwell 615. Further, in order to facilitate the introduction of the culture solution, the depth is preferably not more than 1 time the average value of the maximum value and the minimum value of the opening width of each microwell 615, and is not more than 1/2. It is particularly preferable that In addition, the average value of the maximum and minimum values of the opening width of each microwell 615 is small, and the deeper the depth, the more difficult convection occurs. Therefore, the composition change of the surrounding culture solution is accompanied by the respiration and metabolism of cells. May occur more easily. Since the easiness of growth of cells is affected by the composition of the surrounding culture solution, it is preferable to set the diameter and depth in consideration of biological effects so as to promote the growth of cells. The depth of each microwell 615 is preferably in the range of 50 μm to 500 μm, particularly 50 μm to 300 μm, and particularly 100 μm to 300 μm from the viewpoint of improving workability and stably retaining cultured cells. For example, in the case of the culture container 610 for culturing bovine fertilized eggs, the depth of each microwell 615 is preferably 80 μm or more, more preferably 125 μm or more. The depth of each microwell 615 refers to the distance along the vertical direction from the deepest part of the bottom surface 6151 of the microwell to the opening of the microwell 615.

  Note that it is not essential that the microwell 615 is provided on the bottom surface 613 of the culture container portion 610 of the culture container portion 610, and the bottom surface 613 of the culture container portion may be a flat surface or the entire surface thereof. It may be a concave surface depressed downward (not shown).

  The cleaning container-shaped portion 620 is provided with a cleaning container-shaped portion peripheral wall portion 621 that stands upright from the container bottom portion 110 and opens upward, and is a space surrounded by the cleaning container-shaped portion peripheral wall portion 621. 622 is formed. The cleaning container-shaped portion 620 has a cleaning container-shaped portion bottom surface 623 that forms the bottom of the cleaning container-shaped portion accommodation space 622, and a lower end that is the periphery of the cleaning container-shaped portion bottom surface 623. The cleaning container-shaped portion has a side surface 624 that is connected and has an upper end that surrounds the opening of the cleaning container-shaped portion accommodation space 622.

  In the cleaning container-shaped part 620, the capacity of the cleaning container-shaped part accommodation space 622 is not particularly limited, and can be set to, for example, 10 μm to 1000 μl. The definition of the capacity of the washing container-shaped portion accommodation space 622 is similar to the definition of the capacity of the accommodation portion accommodation space 150, and in the state where the cell treatment container 100 is placed on a flat horizontal surface, the side surface of the washing container-shaped portion is A volume of a space surrounded by an imaginary plane that passes through the lowermost portion of the upper end of 624 and is parallel to the horizontal plane, a bottom surface 623 of the cleaning container-shaped portion, and a side surface 624 of the cleaning container-shaped portion. .

  The shape of the bottom surface 623 of the cleaning container-shaped portion is not particularly limited and may be a flat surface. More preferably, the bottom surface 623 of the cleaning container-shaped portion has a deepest part 625 that is the deepest part of the cleaning container-shaped part 620 and a cleaning container that surrounds the deepest part 625 and is continuous with the deepest part 625. In the cell holding region 626 near the deepest part 625, the cell-shaped region bottom inclined surface 627 is formed so that the inclined surface can surround the cell and hold the cell. With this configuration, the cells to be washed can be collected in the cell holding region 626, and the washing operation of cells using a catheter or pipette becomes easy.

  The cleaning container-shaped portion 620 may be one, but it is preferable that a plurality of cleaning container-shaped portions 620 are provided as illustrated. If a plurality of washing container-shaped portions 620 are provided, a washing liquid is stored in each washing container-shaped portion 620, cells are washed in one washing container-shaped portion 620, and cells after washing are washed. Can be transferred to another cleaning container-shaped portion 620 for further cleaning. The plurality of cleaning container-shaped portions 620 do not have to have the same shape and size, and may have different shapes and sizes from each other.

The washing container 620 can be provided not only for the purpose of washing cells, but also for holding a washing liquid for washing instruments such as catheters and pipettes.
<Embodiment 7 (FIGS. 7A and 7B)>
The cell treatment container 100 of Embodiment 7 shown in FIGS. 7A and 7B is another specific example in which one or more container-shaped portions are provided on the container bottom 110.

  In the seventh embodiment, the cell treatment container 100 of the first embodiment and the like further includes two or more partition walls 701, 702, 703 that stand upright from the container bottom 110 and bridge the housing peripheral wall 140 and the outer peripheral wall 120. , The container-shaped portions 710, 720, 730 have the container bottom portion 110 as a bottom portion, and the surroundings are formed into the accommodating portion peripheral wall portion 140, the outer peripheral wall portion 120, and the adjacent pair of the two or more partition walls 701, 702, 703. It is surrounded and formed. In the illustrated seventh embodiment, three partition walls are provided, and a first partition wall 701, a second partition wall 702, and a third partition wall 704 are used to distinguish them. A bottom surface is formed by the container bottom surface 111 in the first container-shaped portion 710, and the periphery of the container outer wall surface 144, the outer peripheral wall inner peripheral surface 121, one surface 704 of the first partition wall 701, and one of the second partition walls 702. A first container-shaped portion accommodation space 161 which is enclosed by the surface 705 and which is closed at the lower side and is at the upper side is formed. Hereinafter, the accommodating portion outer wall surface 144, the outer peripheral wall portion inner peripheral surface 121, the one surface 704 of the first partition wall 701 and the one surface 705 of the second partition wall 702 will be referred to as “side wall surfaces surrounding the first container-shaped part housing space 161”. Say. A bottom surface is formed by the container bottom surface 111 in the second container-shaped portion 720, and the periphery of the container outer wall surface 144, the outer peripheral wall inner peripheral surface 121, the other surface 706 of the second partition wall 702, and one of the third partition walls 703. A second container-shaped portion accommodating space 162 that is enclosed by the surface 707 and that is closed at the bottom and opened at the top is formed. Hereinafter, the accommodating portion outer wall surface 144, the outer peripheral wall portion inner peripheral surface 121, the other surface 706 of the second partition wall 702, and the one surface 707 of the third partition wall 703 will be referred to as “side wall surface surrounding the second container-shaped portion accommodating space 162”. Say. A bottom surface is formed by the container bottom surface 111 in the third container-shaped portion 730, and the periphery of the container outer wall surface 144, the outer peripheral wall portion inner peripheral surface 121, the other surface 708 of the third partition wall 703, and the other of the first partition wall 701 is surrounded. A third container-shaped portion accommodating space 163 that is enclosed by the surface 709 and that is closed at the bottom and opened at the top is formed. Hereinafter, the accommodating portion outer wall surface 144, the outer peripheral wall portion inner peripheral surface 121, the other surface 708 of the third partition wall 703, and the other surface 709 of the first partition wall 701 are referred to as “side wall surfaces surrounding the third container-shaped portion accommodating space 163”. Say.

  The number of partition walls is not limited to 3 as long as it is 2 or more, and may be 2, 4, 5, 6, or the like.

  The first container-shaped portion 710, the second container-shaped portion 720, and the third container-shaped portion 730, respectively, should be used for the purpose of culturing, washing, etc., similarly to the container-shaped portions 610 and 620 of the sixth embodiment. You can When used for cell culture, the microwell 615 similar to that described in the sixth embodiment is provided on the container bottom surface 111 in the first container-shaped portion 710, the second container-shaped portion 720, or the third container-shaped portion 730. Alternatively, a plurality of layers may be formed (not shown). Further, as shown in a modified example 10 of the seventh embodiment (see FIG. 34), which will be described later, inside the container-shaped portions 710, 720, 730 formed by the accommodating portion peripheral wall portion 140, the outer peripheral wall portion 120, and a pair of adjacent partition walls. Another container-shaped portion may be formed on the container bottom surface 111.

  The number of container-shaped portions formed by the accommodating portion peripheral wall portion 140 and the outer peripheral wall portion 120 and the pair of partition walls adjacent to each other is preferably 2 or more, and more preferably 3 or more. The area of the accommodation space of each container-shaped portion when the cell treatment container 100 is viewed in plan may be uniform or may not be uniform. Each container-shaped portion can be used for different purposes such as cell culture and washing. If there are two or more container-shaped portions, each container-shaped portion can be used for different purposes. Further, in order to be able to perform washing of cells, washing of a catheter, etc., by using different container-shaped portions for each object to be processed, it is preferable that three or more container-shaped portions are formed.

  The respective capacities of the first container-shaped portion accommodation space 161, the second container-shaped portion accommodation space 162, and the third container-shaped portion accommodation space 163 are not particularly limited, but are, for example, in the range of 0.25 mL to 5 mL. The definition of the capacity of each accommodation space 161, 162, 163 is the same as the definition of the capacity of the accommodation space 150 of the accommodation unit 130. The volume of the first container-shaped portion accommodation space 161 (or the volume of the second container-shaped portion accommodation space 162 or the third container-shaped portion accommodation space 164) is in a state in which the cell treatment container 100 is placed on a flat horizontal surface. , The lowermost one of the upper ends of the side wall surface surrounding the first container-shaped portion accommodation space 161 (or the side wall surface surrounding the second container-shaped portion accommodation space 162 or the side wall surface surrounding the third container-shaped portion accommodation space 163). An imaginary plane passing through the positioned portion and parallel to the horizontal plane, the container bottom surface 111, and the side wall surface surrounding the first container-shaped portion accommodation space 161 (or the side wall surface surrounding the second container-shaped portion accommodation space 162, or the third It refers to the volume of the space surrounded by (the side wall surface surrounding the container-shaped portion accommodation space 163).

  The cell treatment container 100 of the seventh embodiment can be used similarly to the cell treatment container 100 of the sixth embodiment, and the same effect can be realized.

  In the cell treatment container of Embodiment 7 and the cell treatment containers of Embodiments 8 to 22 described later, the accommodating portion 130 is formed in the container bottom 110 to accommodate the cells and the cell treatment liquid. It can also be replaced by one or more other receptacles that are open upwards (not shown).

In the cell treatment container 100 of Embodiment 7, the first partition upper end 711, the second partition upper end 712, the third partition upper end 713, the container upper end 145, the outer peripheral wall upper end 122, and the cell processing container 100 up and down from the container bottom 111. Distance along the direction (if the upper ends are not along one plane perpendicular to the vertical direction, the most of the distances of the upper ends from the container bottom surface 111 in the vertical direction of the cell treatment container 100). Short distances) are d ₁ , d ₂ , d ₃ , e and f, respectively. In the cell treatment container 100 of the seventh embodiment, the values of the dimensions d ₁ , d ₂ , d ₃ , e and f are not particularly limited.

However, it is preferable that d ₁ , d ₂ , and d ₃ corresponding to the heights of the partition walls 701, 702, and 703 are ₁ mm to 15 mm independently of each other. When each partition wall 701, 702, 703 supplies the liquid to each accommodation space 161, 162, 163 of the first to third container-shaped portions 710, 720, 730, the partition wall 701, 702, 703 is provided with the liquid. It can be used to bring the tip of the contained catheter or pipette close to each other and to supply while supplying the liquid to the wall surface. At this time, if d ₁ , d ₂ , and d ₃ are 15 mm or less, the liquid is unlikely to leak out of the container, and the operability is excellent. If d ₁ , d ₂ , and d ₃ are 1 mm or more, the storage spaces 161, 162, 163 in contact with the partition walls can store the liquid at a sufficient depth, and thus the storage spaces 161, 162, 163. It is easy to handle the cells and culture solution housed in the container with a pipette, a catheter, or the like.
<Embodiments 7 to 19 (FIGS. 7A, 7B, 8 to 19)>
In the cell treatment container 100 of Embodiment 7, the upper end of the first partition 701 is the first partition upper end 711, the upper end of the second partition 702 is the second partition upper end 712, and the upper end of the third partition 703 is the third partition upper end 713. And In the illustrated example, the first partition upper end 711, the second partition upper end 712, and the third partition upper end 713 are flat surfaces, but the flat surfaces are not essential and may be curved surfaces or ridge lines. Good. Distances along the up-down direction of the cell treatment container 100 from the container lower surface 112 of the outer peripheral wall upper end 122, the container upper end 145, the first partition upper end 711, the second partition upper end 712, and the third partition upper end 713 (each upper end described above). However, in the case where it is not along one plane perpendicular to the vertical direction, the respective shortest distances of the respective upper ends from the container lower surface 112 along the vertical direction of the cell treatment container 100 are a, b, Let c ₁ , c ₂ , and c ₃ .

In the cell treatment container 100 of the seventh embodiment, there is a relation of a>b> c ₁ = c ₂ = c ₃ .

Cell treatment container 100 of the embodiment 8-19 shown in FIG. 8 to 19 _{are, a,} b, cell treatment container 100 of c _1, c 2, _{c 3} of a relative magnitude exemplary relationship except different forms 7 It is a modified example having the same structure as.

In the cell treatment container 100 of Embodiment 8 (FIG. 8), the relationship of a = b = c ₁ = c ₂ = c ₃ is satisfied.

In the cell treatment container 100 of the ninth embodiment (FIG. 9), there is a relationship of c ₁ = c ₂ = c ₃ > a = b.

Embodiment 10 is the relation of the cell treatment container 100, _{a = b> c 1 = c} 2 = c 3 (FIG. 10).

In the cell treatment container 100 of the eleventh embodiment (FIG. 11), the relationship of a> b = c ₁ = c ₂ = c ₃ is satisfied.

In the cell treatment container 100 of the twelfth embodiment (FIG. 12), there is a relationship of b = c ₁ = c ₂ = c ₃ > a.

In the cell treatment container 100 of the thirteenth embodiment (FIG. 13), the relation of a = c ₁ = c ₂ = c ₃ > b is satisfied.

In the cell treatment container 100 of the fourteenth embodiment (FIG. 14), there is a relationship of b> a = c ₁ = c ₂ = c ₃ .

In the cell treatment container 100 of the fifteenth embodiment (FIG. 15), there is a relationship of b>a> c ₁ = c ₂ = c ₃ .

In the cell treatment container 100 according to the sixteenth embodiment (FIG. 16), the relationship of a> c ₁ = c ₂ = c ₃ > b is satisfied.

In the cell treatment container 100 of the seventeenth embodiment (FIG. 17), there is a relation of b> c ₁ = c ₂ = c ₃ > a.

In the cell treatment container 100 of the eighteenth embodiment (FIG. 18), there is a relation of c ₁ = c ₂ = c ₃ >a> b.

In the cell treatment container 100 of the nineteenth embodiment (FIG. 19), there is a relationship of c ₁ = c ₂ = c ₃ >b> a.

Each cell treatment container 100 in _a preferred embodiment 7 to _19, b, by the magnitude of c _1, c 2, _{c 3} are different, different each feature are possible different uses way.
(Characteristic 1) All of the cell treatment containers 100 of Embodiments 7 to 19 are placed on a horizontal surface in a storage space 150, a first container-shaped portion storage space 161, a second container-shaped portion storage space 162, If the liquid is stored in each of the third container-shaped housing spaces 163 within a range that does not exceed the upper end of the surface surrounding the housing spaces, the liquids housed in the housing spaces do not mix with each other. Therefore, it is possible to individually manage cells such as treatment, washing, and culturing in each of the accommodation spaces.
(Feature 2) In the cell treatment container 100 of Embodiments 7, 8, 10, 11, 13, and 16, the container upper end 145, the first partition upper end 711, the second partition upper end 712, and the third partition upper end 713 are independent of each other. Then, the vertical position is the same as the outer peripheral wall upper end 122, or it is lower than the outer peripheral wall upper end 122. Therefore, the outer lid portion 170 that abuts the upper end 122 of the outer peripheral wall portion can cover the entire opening formed by the outer peripheral wall portion 120 of the cell treatment container 100. As an example, FIG. 20 shows a state in which the cell treatment container 100 of Embodiment 8 is covered with the outer lid portion 170. Since the outer lid portion 170 abuts the outer circumferential wall portion upper end 122 over the entire circumference, the cell treatment container 100 can be sealed in a relatively airtight state.

In order to obtain the effect of the feature 2, the upper end 145 of the housing part 130 and the upper ends 711, 712, 713 of the two or more partition walls 701, 702, 703 are independently formed on the outer peripheral wall part 120. It is sufficient to satisfy the condition that the vertical position is the same as that of the upper end 122 of the, or that it is below the upper end 122 of the outer peripheral wall 120.
(Characteristic 3) In the cell treatment container 100 of Embodiments 7, 11, and 16, the upper end of the outer peripheral wall inner peripheral surface 121 has an upper end 145 of the accommodation portion, a first partition upper end 711, a second partition upper end 712, and a third partition upper end 713. Since it protrudes above, the storage space 150, the first container-shaped portion storage space 161, and the second container-shaped portion storage are provided by the inner lid portion 210 whose peripheral edge fits in the inner periphery of the upper end of the outer peripheral wall portion inner peripheral surface 121. The space 162 and the third container-shaped portion accommodation space 163 can be covered. As an example, FIG. 21 shows a state in which the cell treatment container 100 of the eleventh embodiment is covered with the inner lid part 210.

In order to obtain the effect of Feature 3, the upper end 122 of the outer peripheral wall 120 is positioned above the upper end 145 of the housing 130 and the upper ends 711, 712, 713 of each of the two or more partition walls 701, 702, 703. It suffices to satisfy the condition that there is.
(Characteristic 4) In the cell treatment containers 100 of Embodiments 8, 9, 10, 12, 14, 15, 17, and 19, the upper end 145 of the storage portion is the same as the upper end 122 of the outer peripheral wall portion in the vertical direction, or It is above the upper end 122 of the outer peripheral wall. Therefore, in the state where the cell treatment container 100 of Embodiments 8, 9, 10, 12, 14, 15, 17, and 19 is placed on a horizontal plane, the first container-shaped portion housing space 161 and the second container-shaped portion housing Even when the liquid is stored in the space 162 and / or the third container-shaped storage space 163 until the liquid surface reaches the upper end of the outer peripheral wall 120, the liquid moves to the storage space 150 beyond the upper end 145 of the storage portion. Never. For this reason, it is easy to individually carry out operations such as cell treatment, culturing, and washing without the cell treatment liquids being mixed with each other in the accommodating portion 130 and the first to third container-shaped portions 710, 720, 730.

In order to obtain the effect of Feature 4, the upper end 145 of the housing portion 130 has the same vertical position as the upper end 122 of the outer peripheral wall 120, or is located above the upper end 122 of the outer peripheral wall 120. It suffices to satisfy the condition.
(Characteristic 5) In the cell treatment container 100 of Embodiments 7, 11, 13, 16, and 18, the outer peripheral wall upper end 122 is located above the accommodation unit upper end 145. Therefore, with the cell treatment container 100 of Embodiments 7, 11, 13, 16, and 18 placed on a horizontal plane, the first container-shaped portion housing space 161, the second container-shaped portion housing space 162, and the third container. The liquid can be stored in the container storage space 163 until the liquid level exceeds the container upper end 145, and the storage space 150, the first container storage space 161, the second container storage space 162, and A common liquid can be stored in the third container-shaped portion storage space 163. Therefore, it is easy to carry out operations such as cell treatment, culturing, and washing using the cell treatment liquid common to the container 130 and the first to third container-shaped portions 710, 720, 730.

In order to obtain the effect of Feature 5, the condition that the upper end 122 of the outer peripheral wall 120 is above the upper end 145 of the accommodating part 130 may be satisfied.
(Characteristic 6) In the cell treatment container 100 of Embodiments 8, 9, 12, 14, 17, and 19, the container upper end 145, the first partition upper end 711, the second partition upper end 712, and the third partition upper end 713 are independent of each other. Then, it is located at the same vertical position as the outer peripheral wall upper end 122, or is located above the outer peripheral wall upper end 122. Therefore, with the cell treatment container 100 of Embodiments 8, 9, 12, 14, 17, and 19 placed on a horizontal plane, the first container-shaped portion accommodation space 161, the second container-shaped portion accommodation space 162, and / or Alternatively, even when the liquid is stored in the third container-shaped portion storage space 163 up to the upper end of the outer peripheral wall 120, the liquid is stored in the storage portion upper end 145, the first partition upper end 711, the second partition upper end 712, or It does not move beyond the third partition upper end 713 to the adjacent container-shaped portion accommodation spaces 161, 162, 163. For this reason, the cell treatment liquids do not mix with each other in each of the first to third container-shaped portions 710, 720, and 730, and it is easy to individually carry out operations such as cell treatment, culture, and washing.

In order to obtain the effect of the feature 6, the upper end 145 of the housing portion 130 and at least a pair (eg, 701 and 702, 702 and 703, 703 and 701) of at least adjacent ones of the two or more partition walls 701, 702 and 703. As long as the upper ends 711, 712, and 713 are independently located at the same vertical position as the upper end 122 of the outer peripheral wall 120 or above the upper end 122 of the outer peripheral wall 120, the condition is satisfied. Good. More preferably, the upper end 145 of the accommodating part 130 and all the upper ends 711, 712, 713 of the two or more partition walls 701, 702, 703 are independent of the upper end 122 of the outer peripheral wall part 120 and the vertical position. Are the same, or are above the upper end 122 of the outer peripheral wall 120. When a plurality of housings 130 are included, at least one of the upper end 145 of at least one housing 130 and at least two partition walls 701, 702, 703 bridging the at least one housing 130 and the outer peripheral wall 120. The pair of adjacent upper ends 711, 712, 713 may satisfy the above condition.
(Feature 7) In the cell treatment containers 100 of Embodiments 7, 10, 11, 13, 15, 16, and 18, the outer peripheral wall upper end 122 has a first partition upper end 711, a second partition upper end 712, and a third partition upper end 713. Above, or the upper end 122 of the outer peripheral wall is above the upper end 145 of the housing. Therefore, in the state where the cell treatment container 100 of Embodiments 7, 10, 11, 13, 15, 16, 18 is placed on a horizontal plane, the first container-shaped portion accommodation space 161 and the second container-shaped portion accommodation space 162 are provided. Further, the liquid may be stored in the third container-shaped portion storage space 163 until the liquid level exceeds the first partition upper end 711, the second partition upper end 712 and the third partition upper end 713, or the storage portion upper end 145. It is possible, and the common liquid can be stored in the first container-shaped portion storage space 161, the second container-shaped portion storage space 162, and the third container-shaped portion storage space 163. Therefore, it is easy to carry out operations such as culturing and washing of cells in the first to third container-shaped portions 710, 720, 730 using a common cell treatment solution.

In order to obtain the effect of the feature 7, the upper end 122 of the outer peripheral wall 120 is selected from the upper ends 711, 712, 713 of each of the two or more partition walls 701, 702, 703 and the upper end 145 of the housing 130. It suffices to satisfy the condition that it is above at least one. Two or more container-shaped portion accommodation spaces that are adjacent to each other via at least one of the two or more partition walls 701, 702, and 703, or two or more container-shaped portion accommodation spaces that are adjacent to each other via the accommodation portion 130. It is possible to store a common liquid.
(Characteristic 8) In the cell treatment container 100 of Embodiments 9, 12, 14, 15, 17, 18, and 19, the container upper end 145 and / or the first partition upper end 711, the second partition upper end 712, and the third partition upper end 713. Is above the upper end 122 of the outer peripheral wall. Therefore, when these containers further include the outer lid body 170, the outer lid body 170 abuts the accommodation portion upper end 145 and / or the first partition wall upper end 711, the second partition wall upper end 712, and the third partition wall upper end 713, The cell treatment container 100 can be covered without contacting the upper end 122 of the outer peripheral wall. As an example, FIG. 22 shows a state in which the cell treatment container 100 of Embodiment 9 is covered with the outer lid portion 170. Since the outer lid portion 170 does not contact the upper end 122 of the outer peripheral wall portion, it is possible to cover the opening of the cell treatment container 100 in a state where the air permeability between the inside air and the outside air in the container is relatively maintained.
(Characteristic 9) In the cell treatment container 100 of Embodiments 9, 12, 14, 15, 17, 18, and 19, the container upper end 145 and / or the first partition upper end 711, the second partition upper end 712, and the third partition upper end 713. Is above the upper end 122 of the outer peripheral wall. Therefore, in these embodiments, the housing portion 130 and / or the first to third container-shaped portions 710, 720, 730 have wall surfaces that project above the outer peripheral wall portion upper end 122. For this reason, when supplying the liquid to the housing portion 130 and / or the housing spaces of the first to third container-shaped portions 710, 720, 730, the tip of the catheter or pipette containing the liquid is brought close to the protruding wall surface. The liquid can be easily supplied while being transmitted to the protruding wall surface, and the liquid splash at the time of supplying the liquid can be easily suppressed.

  In order to obtain the effects of the features 8 and 9, at least one selected from the upper ends 711, 712, 713 of each of the two or more partition walls 701, 702, 703 and the upper end 145 of the accommodating part 130 is the outer peripheral wall part. It suffices to satisfy the condition that it is above the upper end 122 of 120.

In the illustrated Embodiments 7 to 19, the heights (c ₁ , c ₂ , c ₃ ) from the container bottom surface 112, which correspond to the vertical positions of the upper ends 711, 712, 713 of the respective partition walls 701, 702, 703, are all. It is the same. However, in order to obtain the effects of the features 1 to 9 described above, as long as the conditions described in detail for the features 1 to 9 are satisfied, the upper and lower directions of the upper ends 711, 712, and 713 of the partition walls 701, 702, and 703 are vertical. The positions may be the same or different. Further, when a plurality of housing portions 130 are included, the height (b ₁ ) from the container bottom surface 112, which corresponds to the vertical position of the housing portion upper end 145 of each housing portion 130, may be the same or different. .
<Embodiments 20, 21, 22 (FIGS. 23, 24, 25)>
The cell treatment container 100 of Embodiments 20, 21, and 22 shown in FIGS. 23, 24, and 25 is the cell of Embodiment 7 except that the relative magnitude relationship of a, b, c ₁ , c ₂ , and c ₃ is different. This is a modified example having the same structure as the processing container 100.

In the cell treatment container 100 of Embodiment 20 (FIG. 23), the relationship of a>b> c ₁ = c ₂ > c ₃ is satisfied.

In the cell treatment container 100 of the twenty-first embodiment (FIG. 24), there is a relation of a>b> c ₂ = c ₃ > c ₁ .

In the cell treatment container 100 of Embodiment 22 (FIG. 25), the relationship of a>b> c ₁ = c ₃ > c ₂ is satisfied.

  In the cell treatment container 100 of Embodiment 20, the first partition upper end 711 and the second partition upper end 712 are located above the third partition upper end 713. Therefore, in the state in which the cell treatment container 100 of the twentieth embodiment is placed on the horizontal surface, the liquid level in the second container-shaped portion accommodation space 162 and the third container-shaped portion accommodation space 163 exceeds the third partition upper end 713. However, the liquid can be contained so that the first partition upper end 711 and the second partition upper end 712 are not exceeded. That is, a common liquid can be contained in the second container-shaped portion accommodation space 162 and the third container-shaped portion accommodation space 163, and at the same time, another liquid can be mixed in the first container-shaped portion accommodation space 161. Can be accommodated without. Therefore, in the second and third container-shaped portions 720 and 730, operations such as cell culture and washing are performed using a common liquid, and in the first container-shaped portion 710, cell culture and washing are separately performed using independent liquids. Etc. can be performed.

  In the cell treatment container 100 of Embodiment 21, the second partition upper end 712 and the third partition upper end 713 are located above the first partition upper end 711. Therefore, in the state in which the cell treatment container 100 of the twenty-first embodiment is placed on the horizontal surface, the liquid level exceeds the first partition upper end 711 in the first container-shaped portion accommodation space 161 and the third container-shaped portion accommodation space 163. However, the liquid can be contained so that the second partition upper end 712 and the third partition upper end 713 are not exceeded. That is, a common liquid can be stored in the first container-shaped portion storage space 161 and the third container-shaped portion storage space 163, and at the same time, another liquid can be mixed in the second container-shaped portion storage space 162. Can be accommodated without. Therefore, operations such as cell culture and washing are performed using a common liquid in the first and third container-shaped portions 710 and 730, and cell culture and washing are performed using a separate independent liquid in the second container-shaped portion 720. Etc. can be performed.

  In the cell treatment container 100 of Embodiment 22, the first partition upper end 711 and the third partition upper end 713 are located above the second partition upper end 712. Therefore, in the state in which the cell treatment container 100 of Embodiment 22 is placed on the horizontal surface, the liquid level exceeds the second partition upper end 712 in the first container-shaped portion accommodation space 161 and the second container-shaped portion accommodation space 162. However, the liquid can be contained so that the upper end 711 of the first partition wall and the upper end 713 of the third partition wall are not exceeded. That is, a common liquid can be stored in the first container-shaped portion storage space 161 and the second container-shaped portion storage space 162, and at the same time, another liquid can be mixed in the third container-shaped portion storage space 163. Can be accommodated without. Therefore, in the first and second container-shaped portions 710 and 720, operations such as cell culture and washing are performed using a common liquid, and in the third container-shaped portion 730, cell culture and washing are separately performed using independent liquids. Etc. can be performed.

That is, the cell treatment container 100 according to Embodiments 20, 21, and 22 includes three or more partition walls 701, 702, and 703 that bridge at least one of the housing portions 130 and the outer peripheral wall portion 120, and the three or more partition walls. Reference numerals 701, 702, and 703 include one or two adjacent partition walls A and a pair of partition walls B adjacent to the partition wall A, and the upper ends of the pair of partition walls B are the upper ends of the respective partition walls A. The above effect can be obtained by satisfying the condition that both the upper end of the housing portion 130 and the upper end of the outer peripheral wall portion 120 are above each upper end of the partition wall A. Here, in the twentieth embodiment, the third partition 703 corresponds to the partition A, and the first partition 701 and the second partition 702 correspond to the pair of partition B. In the twenty-first embodiment, the first partition 701 corresponds to the partition A and the second partition 702. The third partition 703 corresponds to the pair of partitions B, and in the twenty-second embodiment, the second partition 702 corresponds to the partition A, and the first partition 701 and the third partition 703 correspond to the pair of partition B. By storing the liquid in the cell treatment container 100 so that the liquid level exceeds the upper ends of the partition walls A, but does not exceed the upper ends of the partition walls B, two or more container-shaped portion accommodation spaces adjacent to each other via the partition walls A are accommodated. It is possible to store a common liquid in.
<Modification 1 of Embodiment 1 (FIG. 26)>
The cell treatment container 100 shown in FIG. 26 is a modified example 1 of the first embodiment, in which the figure formed by the opening of the housing portion 130 in plan view extends from the first portion 261 having a substantially circular shape and the first portion 261 in one direction. It has the same features as the cell treatment container 100 of Embodiment 1 shown in FIG. 1 except that it has a shape in which one existing second portion 262 is combined. According to the first modified example of the first embodiment, the accommodation space 150 has only the second portion 262 as compared with the case where the figure formed in the plan view of the opening of the accommodation portion 130 includes only the substantially first portion 261. Since the cells are expanded, there is an advantage that when the cells accommodated in the accommodation space 150 are handled by an instrument such as a catheter, a region where the instrument can be arranged is expanded and workability is improved.
<Second Modification of First Embodiment (FIG. 27)>
The cell treatment container 100 shown in FIG. 27 is a modified example 2 of the first embodiment, and the figure formed by the opening of the accommodating portion 130 in a plan view has a substantially square first portion 271 and four directions from the first portion 271. Similar to the cell treatment container 100 of the first embodiment shown in FIG. 1 except that it has a shape in which four extended second portions 272-1, 272-2, 272-3, 272-4 are combined. It has characteristics. According to the second modification of the first embodiment, the accommodation space 150 has four second portions 272-in comparison with the case where the figure formed in the plan view of the opening of the accommodation portion 130 includes only the first portion 271 having a substantially square shape. Since only the portions 1, 272-2, 272-3, 272-4 are expanded, when the cells accommodated in the accommodation space 150 are handled by an instrument such as a catheter, the region where the instrument can be arranged is further expanded and workability is improved. There is an advantage of further improvement.
<Other preferred embodiments>
<Other preferred embodiment relating to wall thickness>
In the cell treatment container 100 of the present invention, the thickness of the wall portion containing the resin material forming each portion is not particularly limited, but is preferably 0.3 mm to 3 mm. Specifically, as shown in FIGS. 7A and 7B, the thickness T ₁ of the portion of the accommodation portion peripheral wall portion 140 sandwiched between the accommodation portion side surface 143 and the accommodation portion outer wall surface 144, and the thickness of the first partition wall 701. T _2-1, the thickness of the second partition wall 702 _{T 2-2,} the thickness _{T 2-3} of the third partition wall 703, the thickness _{T 3} of the outer peripheral wall portion 120, and, the thickness of the container bottom 110 0. It is preferably 3 mm to 3 mm. The cell treatment container 100 of the present invention may be sterilized by radiation before shipping. However, when the cell treatment container 100 is formed of a resin material, the resin material changes to yellow due to the irradiation of radiation and has a colorless and transparent property. May be lost. If the thickness of the wall portion containing the resin material in the cell treatment container 100 is 3 mm or less, the colorless and transparent property of the resin material is maintained when the cell treatment container 100 is viewed with the naked eye even when radiation sterilized. It has a suitable appearance. Moreover, if the thickness of each wall is 0.3 mm or more, sufficient strength that is not easily broken when the cell treatment container 100 is used is imparted.
<Other preferred embodiment regarding shape of accommodating portion peripheral wall portion>
Other preferred embodiments of the accommodating portion peripheral wall portion 140 of the cell treatment container 100 of the present invention will be described below as modified examples of the cell treatment container 100 of the seventh embodiment shown in FIGS. 7A and 7B. Other embodiments such as the first embodiment of the cell treatment container 100 of the present invention can be modified examples having similar characteristics.

  FIG. 28 is a schematic diagram of an end surface corresponding to the AA end surface in FIG. 7A of the cell treatment container 100 according to the first modification of the seventh embodiment of the present invention. In the first modified example of the seventh embodiment, the housing portion side surface 143 of the housing portion peripheral wall portion 140 and the housing portion bottom inclined surface 142 are connected by the rounded housing portion side surface-bottom inclined surface connecting curved surface 281. The accommodation part side surface-bottom inclined surface connection curved surface 281 is a accommodation part peripheral wall portion 140 formed by an imaginary plane along the up-down direction (specifically, an imaginary plane including the normal of the accommodation part side surface 143 and along the up-down direction). The accommodating portion side surface 143 and the accommodating portion bottom inclined surface 142 are arranged so that the inclination angle defined in the present specification on the cross section in the vicinity thereof continuously changes from the accommodating portion side surface 143 to the accommodating portion bottom inclined surface 142. Is a curved surface that connects the two surfaces, and is preferably a curved surface having a radius of curvature of 10 μm to 15 mm, more preferably 10 μm to 1 mm on the cross section. In the cell treatment container 100 of the modified example 1 of the seventh embodiment having this feature, the cells 10 enter between the accommodation part side surface 143 and the accommodation part bottom inclined surface 142 more than the embodiment 1 having the bottom surface side crossing portion 147. The possibility can be reduced and it is easy to take out with a catheter or pipette when it gets in. In addition, when the droplets containing the cells 10 are transmitted from the side surface 143 of the containing portion onto the formation surface 141 and moved to the cell holding region 146, the cells 10 can be easily moved.

  28, the container outer wall surface 144 of the container peripheral wall 140 and the container bottom surface 111 of the container bottom 110 have a rounded container outer wall surface-container bottom surface connection curved surface 282. Connected by. The accommodating portion outer wall surface-container bottom surface connecting curved surface 282 is defined by an imaginary plane along the up-down direction (specifically, an imaginary plane including the normal line of the accommodating portion outer wall surface 144 and extending along the up-down direction). A curved surface connecting the outer wall surface 144 of the container and the bottom surface 111 of the container so that the inclination angle defined in the present specification continuously changes from the outer wall surface 144 of the container to the bottom surface 111 of the container on the cross section in the vicinity thereof. And is preferably a curved surface having a radius of curvature of 10 μm to 15 mm, more preferably 10 μm to 1 mm on the cross section. In the cell treatment container 100 of the modified example 7 of the seventh embodiment having this feature, it is possible to reduce the possibility that the cells 10 will enter between the outer wall surface 144 of the container and the bottom surface 111 of the container, and to take them out with a catheter or pipette when the cells 10 enter. Is easy.

  Although not shown, as a further modification of the first modification of the seventh embodiment, there is one having only one of the accommodation part side surface-bottom inclined surface connection curved surface 281 and the accommodation part outer wall surface-container bottom surface connection curved surface 282.

  29A, 29B, and 29C are schematic views of the end surface corresponding to the AA end surface in FIG. 7A of the cell treatment container 100 of the second modification, third, fourth embodiment of the present invention. For convenience of description, only the modified example of the portion sandwiched between the first partition wall 701 and the third partition wall 703 in the storage portion peripheral wall portion 140 of the cell treatment container 100 of the seventh embodiment will be described. Of the portion 140, a portion sandwiched between the first partition wall 701 and the second partition wall 702, a portion sandwiched between the storage second partition wall 702 and the third partition wall 703, and the housing portion peripheral wall portion 140 in the first embodiment. The same modification can be added, and the effect in that case is as follows. It may be understood by substituting the second container-shaped portion accommodation space 162 "and the" peripheral space 160 ".

  In the second modification of the seventh embodiment shown in FIG. 29A, the inclination angle of the accommodation portion side wall 143 of the accommodation portion peripheral wall portion 140 is less than 90 °. The inclination angle of the housing side surface 143 is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. When the inclination angle of the housing portion side surface 143 is within this range, when the cell treatment container 100 is molded by filling a resin material into a die, the cell treatment container 100 is placed on the accommodation portion side surface 143 when the cell treatment container 100 is taken out from the die. It is possible to reduce the possibility of scratches on the skin. In the second modification of the seventh embodiment, the inclination angle of the housing outer wall surface 144 is not particularly limited, but is typically 90 ° (substantially 90 °, including, for example, 89.5 ° to 90.5 °) or 90 °. Is also large, preferably 90 °. When the inclination angle of the housing outer wall surface 144 is within this range, it is possible to prevent the liquid stored in the third container-shaped housing space 163 from overcoming the housing peripheral wall 140 and flowing into the housing space 150. it can.

  29B, the inclination angle of the housing outer wall surface 144 of the housing peripheral wall portion 140 is less than 90 °. The inclination angle of the housing outer wall surface 144 is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. When the inclination angle of the housing part outer wall surface 144 is within this range, when the cell treatment container 100 is molded by filling a resin mold into a mold, the housing part outer wall surface is taken out from the mold. The possibility of scratches on the 144 can be reduced. In the third modification of the seventh embodiment, the inclination angle of the housing portion side surface 143 is not particularly limited, but is typically 90 ° (substantially 90 °, including, for example, 89.5 ° to 90.5 °) or more than 90 °. Large, preferably 90 °. When the inclination angle of the housing portion side surface 143 is in this range, it is possible to prevent the liquid stored in the housing space 150 of the housing portion 130 from passing over the housing portion peripheral wall portion 140 and flowing into the third container-shaped portion housing space 163. can do.

  In the fourth modification of the seventh embodiment shown in FIG. 29C, the inclination angle of the accommodation portion side surface 143 of the accommodation portion peripheral wall portion 140 is less than 90 °. The inclination angle of the housing side surface 143 is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. At this time, similarly to the first modification of the seventh embodiment, the possibility of scratches on the side surface 143 of the housing portion can be reduced. In the fourth modification of the seventh embodiment, the inclination angle of the housing outer wall surface 144 is less than 90 °. The inclination angle of the housing outer wall surface 144 is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. At this time, similarly to the second modification of the seventh embodiment, the possibility of scratches on the outer wall surface 144 of the housing portion can be reduced.

  Although not shown, as another modification of the seventh embodiment, the inclination angle of the accommodation portion side surface 143 of the accommodation portion peripheral wall portion 140 and the inclination angle of the accommodation portion outer wall surface 144 are independently 90 ° (substantially 90 °). , Including 99.5 ° to 90.5 °) or 90 °, preferably 90 °. In this modification, the liquid stored in the storage space 150 of the storage portion 130 passes over the storage portion peripheral wall portion 140 and flows into the third container-shaped portion storage space 163, and the liquid is stored in the third container-shaped portion storage space 163. It is possible to prevent the stored liquid from overcoming the storage portion peripheral wall portion 140 and flowing into the storage space 150.

In each of the modified examples described in detail in this section, it is preferable that the inclination angle is within each of the above ranges over the entire height direction of the housing portion side surface 143, but it is not essential, and at least the housing portion side surface 143 is at least the housing portion. The inclination angle at the height position near the upper end 145 may be within the above range. Similarly, in each of the modified examples described in detail in this section, it is preferable that the inclination angle is within the above ranges over the entire height direction of the housing outer wall surface 144, but it is not essential, and the housing outer wall surface 144 is not essential. Of these, the inclination angle at least at the height position near the upper end 145 of the accommodating portion may be within the above range.
<Other preferred embodiment (1) regarding the shape of the partition wall>
Another preferred modified example of the partition walls 701, 702, 703 of the cell treatment container 100 of Embodiment 7 of the present invention will be described below. For convenience of description, only the first partition 701 will be described, but the other second partition 702 and the third partition 703 may have the same characteristics.

  FIG. 30 is a schematic diagram of an end surface corresponding to the BB end surface in FIG. 7A of the cell treatment container 100 of the fifth modification of the seventh embodiment of the present invention. In the fifth modification of the seventh embodiment, one surface 704 of the first partition wall 701 and the container bottom surface 111 of the container bottom portion 110 are connected by the first rounded partition wall side surface-bottom surface connection curved surface 301. The first partition wall side surface-bottom surface connection curved surface 301 is defined by an imaginary plane extending in the up-down direction (specifically, an imaginary plane including the normal to one surface 704 of the first partition 701 and extending in the up-down direction). The first partition wall so that the inclination angle defined in this specification continuously changes from one surface 704 of the first partition wall 701 to the container bottom surface 111 of the container bottom portion 110 on the cross section of the one partition wall 701 and its vicinity. It is a curved surface that connects one surface 704 of 701 and the container bottom surface 111 of the container bottom portion 110, and is preferably a curved surface having a radius of curvature of 10 μm to 15 mm, more preferably 10 μm to 1 mm on the cross section. In the cell treatment container 100 of the fifth modification of the seventh embodiment having this feature, the possibility of the cells 10 entering between the one surface 704 of the first partition wall 701 and the container bottom surface 111 of the container bottom 110 can be reduced, and the cells 10 can enter. In this case, it is easy to take out with a catheter or pipette.

Further, in the fifth modified example of the seventh embodiment, the other surface 709 of the first partition wall 701 and the container bottom surface 111 of the container bottom portion 110 are connected by the second rounded partition wall side surface-bottom surface connection curved surface 302. The second partition wall side surface-bottom surface connecting curved surface 302 is defined by an imaginary plane extending in the up-down direction (specifically, an imaginary plane including the normal line of the other surface 709 of the first partition 701 and extending in the up-down direction). The first partition wall so that the inclination angle defined in this specification continuously changes from the other surface 709 of the first partition wall 701 to the container bottom surface 111 of the container bottom portion 110 on the cross section of the first partition wall 701 and its vicinity. It is a curved surface that connects the other surface 709 of 701 and the container bottom surface 111 of the container bottom portion 110, and preferably has a curvature radius of 10 μm to 15 mm, more preferably 10 μm to 1 mm on the cross section. In the cell treatment container 100 according to the fifth modification of the seventh embodiment having this feature, it is possible to reduce the possibility of the cells 10 entering between the other surface 709 of the first partition wall 701 and the container bottom surface 111 of the container bottom portion 110, and to enter. In this case, it is easy to take out with a catheter or pipette.
<Other preferred embodiment (2) regarding shape of partition wall>
Still another preferable modified example of the partition walls 701, 702, 703 of the cell treatment container 100 of Embodiment 7 of the present invention will be described below. For convenience of description, only the third partition wall 703 will be described, but the other first partition wall 701 and the second partition wall 702 can also have similar features.

  FIG. 31 is a plan view of a region corresponding to the region 310 in FIG. 7A of the cell treatment container 100 according to Modification 6 of Embodiment 7 of the present invention. In the seventh modified example 6, one surface 707 of the third partition wall 703 and the housing outer wall surface 144 of the housing circumferential wall portion 140 are formed by the rounded first partition wall side surface-housing outer wall connection curved surface 311. It is connected, and one surface 707 of the third partition wall 703 and the outer peripheral wall portion inner peripheral surface 121 of the outer peripheral wall portion 120 are connected by the first rounded partition wall side surface-outer peripheral wall portion inner peripheral surface connection curved surface 313. ing. The first partition wall side surface-accommodating portion outer wall surface connection curved surface 311 is accommodated with the one surface 707 of the third partition wall 703 on the cross section of the third partition wall 703 and the vicinity thereof by a virtual plane along a direction perpendicular to the vertical direction. It is a curved surface that smoothly connects the outer wall surface 144 of the housing wall of the peripheral wall portion 140, and preferably has a curvature radius of 10 μm to 15 mm on the cross section. Similarly, the first partition wall side surface-outer peripheral wall portion inner peripheral surface connection curved surface 313 is one of the third partition walls 703 on the cross section of the third partition wall 703 and the vicinity thereof by a virtual plane along a direction perpendicular to the vertical direction. It is a curved surface that smoothly connects the surface 707 and the inner peripheral surface 121 of the outer peripheral wall portion 120, and is preferably a curved surface having a radius of curvature of 10 μm to 15 mm on the cross section. The presence of the first partition wall side surface-container outer wall surface connection curved surface 311 may cause the cells 10 to enter between one surface 707 of the third partition wall 703 and the container outer wall surface 144 of the container peripheral wall portion 140. In addition, it is easy to remove with a catheter or pipette when it enters. Similarly, in the first partition wall side surface-outer peripheral wall portion inner peripheral surface connection curved surface 313, there is a possibility that the cells 10 may enter between one surface 707 of the third partition wall 703 and the outer peripheral wall portion inner peripheral surface 121 of the outer peripheral wall portion 120. It is possible to reduce the number and it is easy to take out with a catheter or pipette when it enters.

  The first partition wall side surface-accommodating portion outer wall surface connection curved surface 311 and the first partition wall side surface-outer peripheral wall portion inner peripheral surface connection curved surface 313 can also exhibit the following preferable effects. When the first partition wall side surface-accommodating portion outer wall surface connection curved surface 311 and the first partition wall side surface-outer peripheral wall portion inner peripheral surface connection curved surface 313 do not exist, the following problems exist. That is, in the case where a liquid such as oil, cell washing liquid, and culture liquid is stored in the second container-shaped storage space 162 of the cell treatment container 100, one surface 707 of the third partition 703 and the peripheral wall 140 of the storage part. When the housing outer wall surface 144 and the outer peripheral wall inner peripheral surface 121 of the outer peripheral wall 120 have an affinity for the liquid to be contained (for example, the liquid is oil, and each surface is a hydrophobic surface). In some cases), a portion where one surface 707 of the third partition wall 703 and the housing outer wall surface 144 of the housing peripheral wall portion 140 intersect, and one surface 707 of the third partition wall 703 and the outer peripheral wall portion 120. There is a problem that the liquid diffuses along the portion where the outer peripheral wall portion inner peripheral surface 121 intersects and moves from the second container-shaped portion accommodation space 162 to the adjacent third container-shaped portion accommodation space 163 (Experiment 6 reference). This problem can be solved by providing the first partition wall side surface-accommodating portion outer wall surface connection curved surface 311 and the first partition wall side surface-outer peripheral wall portion inner peripheral surface connection curved surface 313.

  In the present specification, that a surface has an affinity for a liquid means that the contact angle of the liquid on the surface is preferably 45 ° or less, more preferably 30 ° or less. . That a surface has no affinity for a liquid means that the contact angle of the liquid on the surface is preferably 60 ° or more, more preferably 80 ° or more. The measurement temperature of the contact angle may be measured at a temperature at which the cell treatment container 100 is assumed to be used, for example, in the range of 20 ° C to 40 ° C.

31, the other surface 708 of the third partition wall 703 and the storage portion outer wall surface 144 of the storage portion peripheral wall portion 140 are rounded second partition wall side surface-storage portion outer wall surface. The other side surface 708 of the third partition wall 703 and the outer peripheral wall portion inner peripheral surface 121 of the outer peripheral wall portion 120, which are connected by the connection curved surface 312, are rounded second partition wall side surface-outer peripheral wall portion inner peripheral surface connection curved surface. They are connected by 314. The second partition wall side surface-accommodating portion outer wall connection curved surface 312 is accommodated with the other surface 708 of the third partition wall 703 on the cross section of the third partition wall 703 and the vicinity thereof by a virtual plane along a direction perpendicular to the vertical direction. It is a curved surface that smoothly connects the outer wall surface 144 of the accommodating portion of the peripheral wall portion 140, and is preferably a curved surface having a radius of curvature of 10 μm to 15 mm on the cross section. Similarly, the second partition wall side surface-outer peripheral wall portion inner peripheral surface connection curved surface 314 is the other of the third partition walls 703 on the cross section of the third partition wall 703 and the vicinity thereof by a virtual plane along a direction perpendicular to the vertical direction. It is a curved surface that smoothly connects the surface 708 and the outer peripheral wall portion inner peripheral surface 121 of the outer peripheral wall portion 120, and is preferably a curved surface having a radius of curvature of 10 μm to 15 mm on the cross section. The second partition wall side surface-accommodating portion outer wall surface connecting curved surface 312 and the second partition wall side surface-outer peripheral wall portion inner peripheral surface connecting curved surface 314 are respectively the first partition wall side surface-accommodating portion outer wall surface connecting curved surface 311 and the first partition wall. The same effect as that of the side surface-outer peripheral wall portion inner peripheral surface connection curved surface 313 can be obtained.
<Other preferred embodiment (3) regarding the shape of the partition wall>
Still another preferable modified example of the partition walls 701, 702, 703 of the cell treatment container 100 of Embodiment 7 of the present invention will be described below. For convenience of description, only the first partition 701 will be described, but the other second partition 702 and the third partition 703 may have the same characteristics. In that case, the "first partition 701" in the following description is referred to as "first partition". 2 partition 702 ”or“ 3rd partition 703 ”as appropriate, and“ one surface 704 ”and“ other surface 709 ”as appropriate as“ 2nd partition 702 ”or“ 3rd partition 703 ”. Just understand.

  32A, 32B, and 32C are schematic views of an end surface corresponding to the BB end surface in FIG. 7A of the cell treatment container 100 according to the seventh modified examples 7, 8, and 9 of the present invention.

  32A, the inclination angle of the one surface 704 of the first partition wall 701 is less than 90 °. The inclination angle of the one surface 704 is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. When the inclination angle of the one surface 704 is in this range, when the cell treatment container 100 is molded by filling a resin material into a mold, the one surface 704 is removed when the cell treatment container 100 is taken out of the mold. It is possible to reduce the possibility of scratches on the skin. In the seventh modification of the seventh embodiment, the inclination angle of the other surface 709 of the first partition wall 701 is not particularly limited, but is typically 90 ° (substantially 90 °, including, for example, 89.5 ° to 90.5 °). Or greater than 90 °, preferably 90 °. When the inclination angle of the other surface 709 of the first partition wall 701 is in this range, the liquid contained in the third container-shaped portion accommodation space 163 flows over the first partition wall 701 and flows into the first container-shaped portion accommodation space 161. Can be suppressed.

  32B, the inclination angle of the other surface 709 of the first partition 701 is less than 90 °. The inclination angle of the other surface 709 is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. When the inclination angle of the other surface 709 is within this range, when the cell treatment container 100 is molded by filling a resin material into a mold, the other surface 709 is removed when the cell treatment container 100 is taken out from the mold. It is possible to reduce the possibility of scratches on the skin. In the eighth modification of the seventh embodiment, the inclination angle of the one surface 704 of the first partition wall 701 is not particularly limited, but is typically 90 ° (substantially 90 °, including, for example, 89.5 ° to 90.5 °). Or greater than 90 °, preferably 90 °. When the inclination angle of the one surface 704 of the first partition wall 701 is within this range, the liquid contained in the first container-shaped portion accommodation space 161 passes over the first partition wall 701 and flows into the third container-shaped portion accommodation space 163. Can be suppressed.

  32C, the inclination angle of the one surface 704 of the first partition 701 is less than 90 °. The inclination angle of the one surface 704 is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. At this time, similarly to the modified example 7 of the seventh embodiment, the possibility of scratches on the one surface 704 can be reduced. In the ninth modification of the seventh embodiment, the inclination angle of the other surface 709 of the first partition 701 is less than 90 °. The inclination angle of the other surface 709 is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. At this time, the possibility of scratches on the one surface 709 can be reduced as in the eighth modified example of the seventh embodiment.

  Although not shown, as another modification of the seventh embodiment, the inclination angle of one surface 704 of the first partition wall 701 and the inclination angle of the other surface 709 of the first partition wall 701 are independently 90 ° (substantially 90 °, for example, 90 °, for example). (Including 89.5 ° to 90.5 °) or 90 °, preferably 90 °. In this modified example, the liquid stored in the third container-shaped portion storage space 163 flows over the first partition wall 701 and flows into the first container-shaped portion storage space 161, and the liquid is stored in the first container-shaped portion storage space 161. It is possible to prevent the stored liquid from overcoming the first partition wall 701 and flowing into the third container-shaped portion storage space 163.

In each of the modified examples described in detail in this section, it is preferable that the inclination angle is within the above ranges over the entire one surface 704 of the first partition 701 in the height direction, but it is not essential. The inclination angle at least at the height position near the upper end 711 of the first partition on the one surface 704 may be within the above range. Similarly, in each of the modifications described in detail in this section, it is preferable that the inclination angle is within the above ranges over the entire height direction of the other surface 709 of the first partition wall 701, but it is not essential. It suffices that the inclination angle at least at the height position near the upper end 711 of the first partition wall of the other surface 709 of the first partition wall 701 is within the above range.
<Other preferred embodiment (4) regarding the shape of the partition wall>
FIG. 33 shows an example in which the cell treatment container 100 according to the seventh embodiment of the present invention includes an outer lid portion 170. A surface of the outer lid portion 170 facing the inside of the container is referred to as a lid inner surface 331.

In the example of the cell treatment container 100 of the seventh embodiment shown in FIG. 33, the heights d ₁ , d ₂ , and d _{3 of} the first partition wall 701, the second partition wall 702, and the third partition wall 703 from the container bottom surface 111 and the peripheral wall of the housing portion. The height e of the portion 140 from the container bottom surface 111 is independently smaller than the height f of the outer peripheral wall 120 from the container bottom surface 111, or at most the same as f. For convenience of description, only the first partition 701 will be described, but the other second partition 702 and the third partition 703 can also have the same characteristics. In that case, the "first partition 701" in the following description is referred to as "first partition". It may be understood by appropriately replacing “d ₁ ” with “d ₂ ” or “d ₃ ” in the second partition 702 ”or the“ third partition 703 ”.

In the cell treatment container 100 according to the seventh embodiment shown in FIG. 33, one surface 704 of the first partition wall 701 has a surface having an affinity for the liquid contained in the first container-shaped portion accommodation space 161 (for example, the liquid). Is oil, one surface 704 of the first partition wall 701 is a hydrophobic surface such as a styrene surface not subjected to hydrophilic treatment, and the inner lid surface 331 has an affinity for the liquid. In the case of the surface, the height d ₁ of the first partition wall 701 from the container bottom surface 111 is preferably a value smaller than f by 1 mm or more. The liquid easily diffuses on the surface of the substrate having affinity, but if d ₁ is a value smaller than f by 1 mm or more, the liquid is stored in the first container-shaped portion storage space 161 up to the upper end 711 of the first partition 701. Even when the outer lid portion 170 is used as the lid, the liquid is less likely to travel along the lid inner surface 331, get over the first partition wall 701, and move to the third container-shaped portion accommodation space 163. In this mode, the entire “side wall surface surrounding the first container-shaped portion accommodation space 161” including the one surface 704 of the first partition 701, the first partition upper end 711, the other surface 709 of the first partition 701, and the container It is particularly suitable when the region of the bottom surface 111 inside the first container-shaped portion accommodation space 161 is a surface having an affinity for the liquid. The lower limit of d ₁ is not particularly limited, but is preferably a value larger than 2 mm, more preferably 3 mm or more, and further preferably 8 mm or more. The entire “side wall surface surrounding the first container-shaped portion accommodation space 161” including the one surface 704 of the first partition wall 701, and the region of the container bottom surface 111 within the first container-shaped portion accommodation space 161 is compatible with the liquid. As a method of using the cell treatment container 100 of the present invention, which is a surface having a property, a first liquid containing cells and the like having no affinity with the region (for example, when the region is hydrophobic, water or the like is used). A drop of a hydrophilic liquid) is formed on the region, and a second liquid that has no affinity with the drop and has an affinity with the region or the like is formed so as to cover the drop, and the first container-shaped portion accommodation space There is a method of using it in 161. For example, the first liquid is a cell treatment liquid based on water containing cells 10 and the second liquid is oil. The amount of the first liquid forming the drop is generally 15 μL or more. When a drop of 15 μL of the first liquid is formed on the container bottom surface 111 that has no affinity for the first liquid, the height from the container bottom surface 111 to the drop top is about 2 mm (see Experiment 7). Therefore, if d ₁ is a value larger than 2 mm, particularly 3 mm or more, it is possible to cover the entire drop of the first liquid having a volume of 15 μL or more with the second liquid. Further, when d ₁ is 8 mm or more, it is suitable for preventing liquid leakage of the second liquid.

Further, in the cell treatment container 100 of the seventh embodiment shown in FIG. 33, one surface 704 of the first partition wall 701 is a surface having an affinity for the liquid stored in the first container-shaped portion storage space 161. In addition, when the lid inner surface 331 is a surface having no affinity for the liquid (for example, the liquid is oil and the lid inner surface 331 is a hydrophilically treated surface), the first partition wall 701 The height d ₁ from the container bottom surface 111 can be the same value as f or a smaller value. In this case, since the liquid is less likely to diffuse along the inner surface 331 of the lid, the liquid is less likely to travel along the inner surface 331 of the lid, get over the first partition wall 701, and move to the third container-shaped portion accommodation space 163. In this mode, the entire “side wall surface surrounding the first container-shaped portion accommodation space 161” including the one surface 704 of the first partition 701, the first partition upper end 711, the other surface 709 of the first partition 701, and the container It is particularly suitable when the region of the bottom surface 111 inside the first container-shaped portion accommodation space 161 is a surface having an affinity for the liquid. The lower limit of d ₁ is not particularly limited, but is preferably greater than 2 mm, more preferably 3 mm or more, and further preferably 8 mm or more for the same reason as above.

Furthermore, in the cell treatment container 100 of the seventh embodiment shown in FIG. 33, one surface 704 of the first partition wall 701 does not have an affinity for the liquid contained in the first container-shaped portion accommodation space 161. When the surface is a surface (for example, the liquid is oil and one surface 704 of the first partition wall 701 is hydrophilically treated), the height d ₁ of the first partition wall 701 from the container bottom surface 111 is f. It can be the same value or a smaller value. In this aspect, since it is difficult for the liquid to diffuse on the one surface 704 of the first partition wall 701, the liquid is less likely to move over the first partition wall 701 and move to the third container-shaped portion accommodation space 163. . In this mode, the entire “side wall surface surrounding the first container-shaped portion accommodation space 161” including the one surface 704 of the first partition 701, the first partition upper end 711, the other surface 709 of the first partition 701, and the container It is particularly suitable when the region in the first container-shaped portion accommodation space 161 of the bottom surface 111 is a surface that has no affinity for the liquid. The lower limit of d ₁ is not particularly limited, but is preferably greater than 2 mm, more preferably 3 mm or more, and further preferably 8 mm or more for the same reason as above.
<Other preferred embodiment relating to container-shaped portion>
FIG. 34 is a plan view of the cell treatment container 100 of Modification 10 of the seventh embodiment of the present invention.

  In the cell treatment container 100 of Modification Example 7 of the seventh embodiment, another container-shaped portion is formed in the container-shaped portion surrounded by the peripheral wall portion of the accommodating portion, the peripheral wall portion, and the pair of adjacent partition walls.

In the illustrated example, a further container-shaped portion 610 for culture is formed on the container bottom 110 that constitutes the bottom of the first container-shaped portion 710. The specific configuration of the culture vessel-shaped portion 610 is as described above with respect to the sixth embodiment. A cell and a culture solution are stored in a culture container-shaped portion accommodation space 612 inside the culture container-shaped portion 610, and the first container-shaped portion accommodation space 161 of the first container-shaped portion 710 is filled with oil for culture. The entire container-shaped portion 610 can be covered. At this time, other operations such as cell washing and catheter washing can be performed in the adjacent container-shaped portions such as the second container-shaped portion 720 and the third container-shaped portion 730. The number of the additional vessel-shaped portion for culture 610 is not particularly limited and may be one or plural. The height of the upper end 616 of the culture container-shaped portion peripheral wall portion 611 of the further culture container-shaped portion 610 from the container bottom surface 611 is not particularly limited, but at most, among the above d ₁ , d ₂ , e and f. It is preferably the same as or smaller than the smallest value, and preferably 1 mm or more. If it is at most the upper limit value, the first container-shaped portion housing space 161 can be filled with oil so as to cover the entire first container-shaped portion 710. If it is at least the above lower limit, it is easy to handle the cells and cell treatment liquid stored in the further culture vessel 610 with a pipette or a catheter.

In the illustrated example, a further cleaning container-shaped portion 620 is further formed on the container bottom portion 110 that constitutes the bottom portion of the third container-shaped portion 730. The specific configuration of the further cleaning container 620 is as described above with respect to the sixth embodiment. The number of the additional cleaning container-shaped portion 620 is not particularly limited and may be one or plural.
<Other preferred embodiment (1) regarding the shape of the outer peripheral wall>
Another preferable modification of the outer peripheral wall portion 120 of the cell treatment container 100 according to the seventh embodiment of the present invention will be described below.

FIG. 35 is a schematic view of an end surface corresponding to the CC end surface of FIG. 7A of the cell treatment container 100 of Modification 11 of the seventh embodiment of the present invention. In the eleventh modified example of the seventh embodiment, the outer peripheral wall portion inner peripheral surface 121 of the outer peripheral wall portion 120 and the container bottom surface 111 of the container bottom portion 110 are connected by a rounded outer peripheral wall portion inner peripheral surface-bottom surface connecting curved surface 351. The inner peripheral surface-bottom surface connecting curved surface 351 of the outer peripheral wall portion is an outer peripheral wall portion 120 formed by a virtual plane along the up-down direction (specifically, a virtual plane including the normal line of the outer peripheral wall portion inner peripheral surface 121 and extending along the up-down direction). On the cross section in the vicinity thereof, the inclination angle defined in the present specification is such that the inclination angle of the outer peripheral wall portion 120 continuously changes from the outer peripheral wall portion inner peripheral surface 121 of the outer peripheral wall portion 120 to the container bottom surface 111 of the container bottom portion 110. It is a curved surface that connects the inner peripheral surface 121 of the outer peripheral wall portion and the container bottom surface 111 of the container bottom portion 110, and preferably has a curvature radius of 10 μm to 15 mm, more preferably 10 μm to 1 mm on the cross section. In the cell treatment container 100 of the modified example 7 of the seventh embodiment having this feature, it is possible to reduce the possibility that the cells 10 enter between the outer peripheral wall inner peripheral surface 121 of the outer peripheral wall 120 and the container bottom surface 111 of the container bottom 110. It is easy to take out with a catheter or pipette when it gets inside.
<Other preferred embodiment (2) regarding the shape of the outer peripheral wall portion>
Still another preferable modified example of the outer peripheral wall portion 120 of the cell treatment container 100 according to the seventh embodiment of the present invention will be described below.

  FIG. 36 is a schematic view of an end face corresponding to the C-C end face in FIG. 7A of the cell treatment container 100 of Modification 12 of the seventh embodiment of the present invention. For convenience of explanation, only a modified example of the portion sandwiched between the first partition wall 701 and the third partition wall 703 in the outer peripheral wall portion 120 of the cell treatment container 100 of the seventh embodiment will be described, but the outer peripheral wall portion 120. Of the above, a portion sandwiched between the first partition wall 701 and the second partition wall 702, a portion sandwiched between the storage second partition wall 702 and the third partition wall 703, and the outer peripheral wall portion 120 according to the first embodiment are similarly modified. The effect in that case can be added by adding the “third container-shaped portion accommodation space 163” to the “first container-shaped portion accommodation space 161” and the “second container”, respectively, in the description of the effects according to the following modifications. It should be understood by substituting the “shaped portion accommodation space 162” and the “outer peripheral space 160”.

  In Modification 12 of Embodiment 7 shown in FIG. 36, the inclination angle of the outer peripheral wall inner peripheral surface 121 of the outer peripheral wall 120 is less than 90 °. The inclination angle of the inner peripheral surface 121 of the outer peripheral wall portion is more preferably 70 ° to 89 °, particularly preferably 83 ° to 85 °, and most preferably 85 °. When the inclination angle of the inner peripheral surface 121 of the outer peripheral wall is in this range, when the cell treatment container 100 is molded by filling a resin material into a mold, the inner wall of the outer peripheral wall is taken out when the cell treatment container 100 is taken out from the mold. The possibility of scratches on the peripheral surface 121 can be reduced.

  Although not shown, as another modified example of the seventh embodiment, the inclination angle of the outer peripheral wall inner peripheral surface 121 of the outer peripheral wall 120 is 90 ° (substantially 90 °, for example, 89.5 ° to 90.5 °). Included) or greater than 90 °, preferably 90 °. In this modification, it is possible to prevent the liquid stored in the third container-shaped storage space 163 from overcoming the outer peripheral wall 120 and flowing out of the container.

  Although not shown, as another modification of the seventh embodiment, the inclination angle of the outer peripheral wall outer peripheral surface 124 of the outer peripheral wall 120 is less than 90 °, more preferably 70 ° to 89 °, and particularly preferably 83 ° to 85. The most preferable example is 85 °, and most preferably 85 °. When the inclination angle of the outer peripheral wall portion outer peripheral surface 124 is within this range, when the cell treatment container 100 is molded by filling a resin material into a mold, the outer peripheral wall portion is taken out from the mold. The possibility of scratches on the outer peripheral surface 124 can be reduced.

In each of the modified examples described in detail in this section, it is preferable that the inclination angle is within the above ranges over the entire height of the outer peripheral wall portion inner peripheral surface 121, but it is not essential. Of these, the inclination angle at the height position near the upper end 122 of the outer peripheral wall may be within the above range. Similarly, in each of the modified examples described in detail in this section, it is preferable that the inclination angle is within each of the above ranges over the entire height of the outer peripheral wall outer peripheral surface 124, but it is not essential. The inclination angle at the height position near the upper end 122 of the outer peripheral wall of the surface 124 may be within the above range.
<Other preferred embodiment (3) regarding the shape of the outer peripheral wall>
In the cell treatment container 100 of the present invention, the height a of the outer peripheral wall portion upper end 122 of the outer peripheral wall portion 120 from the container lower surface 112 is not particularly limited, but is preferably 15 mm or less. When a is 15 mm or less, a plurality of cell treatment containers 100, for example, 5 to 15, particularly 10 cells can be stably placed even when they are placed on top of each other. The lower limit of a is not particularly limited, but is preferably 1 mm or more. If a is 1 mm or more, it is easy to handle the cells or cell treatment solution contained in the cell treatment container 100 with a pipette or a catheter.
<Other preferred embodiment (4) regarding the shape of the outer peripheral wall>
FIG. 37A shows a plan view of a cell treatment container 100 of the present invention according to Modification 13 of Embodiment 7. FIG. 37B shows a schematic cross-sectional view of the cell treatment container 100 of the present invention according to the thirteenth modified example of the seventh embodiment, taken along the line D-D shown in FIG. 37A.

  In the thirteenth modification of the seventh embodiment, the outer peripheral wall portion 120 has the following features.

  In the thirteenth modification of the seventh embodiment, the outer peripheral wall outer peripheral surface 124 located on the outer peripheral side of the outer peripheral wall 120 has a stepped structure in which at least one step is formed. Specifically, a portion of the outer peripheral wall portion outer peripheral surface 124 near the lower end includes an inclined surface 124 ′ having a shape in which the peripheral diameter increases as the inclination angle increases upward by more than 90 °. A portion 124 ′ ″ near the upper end and the inclined surface 124 ′ are connected by a connection surface 124 ″ to form a step. Since the outer peripheral wall outer peripheral surface 124 has the stepped structure, the operation of manually moving the cell treatment container 100 is facilitated, and the risk of the operator dropping the cell treatment container 100 can be reduced.

  Further, in the thirteenth modification of the seventh embodiment, a bottom protrusion 125 that protrudes below the container bottom lower surface 113 of the container bottom 110 is formed at the lower end of the outer peripheral wall 120 over the entire circumference. The bottom surface 112 of the container is defined by the lower end of the bottom protrusion 125.

  Further, in the thirteenth modification of the seventh embodiment, a dent portion 371 formed by denting the outer peripheral wall portion 120 inward is present in a part of the outer peripheral wall portion 120. FIG. 37C is a perspective view of the outer peripheral wall portion 120 of the cell treatment container 100 in the vicinity of the recessed portion 371. The recessed portion 371 is an example of marking for identifying the direction, position, etc. of the cell treatment container 100. During microscopic observation, the direction and position of the cell treatment container 100 can be easily grasped based on the marking. The type of marking is not particularly limited, and may be identifiable by contact, visually identifiable, or identifiable by other means. . The number of markings included in the cell treatment container 100 may be one or plural. In the cell processing container 100 of the seventh modification, the operator can touch the outer peripheral wall outer peripheral surface 124 and identify the direction and position of the cell processing container 100 based on the recess 371.

Although not shown, in a seventh modified example 13 ′ which is a further modified example of the seventh modified example of the cell treatment container 100 of the present invention, a height b of the container upper end 145 from the container lower surface 112 and 1 the height _{c 1} from the container bottom surface 112 of the partition wall upper end 711, the height _{c 2} from the container bottom surface 112 of the second partition wall upper end 712, the height _{c 3} from the container bottom surface 112 of the third partition upper edge 713 It is the same, and more preferably b, c ₁ , c ₂ , c ₃ is smaller than the height a of the outer peripheral wall upper end 122 from the container lower surface 112, and the outer peripheral wall upper end 122, the housing upper end 145, and the first The relative height relationship between the partition top 711, the second partition top 712, and the third partition top 713 is the same as in FIG. In Embodiment 13 Modification 13 ′, the heights d ₁ , d ₂ , and d _{3 of} the first partition wall 701, the second partition wall 702, and the third partition wall 703 from the container bottom surface 111, and the container bottom surface 111 of the accommodating part peripheral wall portion 140. The height e is preferably the same, and its value is not particularly limited, but can be 4 mm.
<Other preferred embodiment (1) regarding marking>
FIG. 38 shows a plan view of the cell treatment container 100 according to the fourteenth modification of the seventh embodiment. The D-D cross section is the same as that shown in FIG. 37B for the thirteenth modification of the seventh embodiment.

The modification 14 of the seventh embodiment is also an example in which the marking is provided on the outer peripheral wall portion 120, and specifically includes a marking protrusion 381 formed by protruding a part of the outer peripheral wall portion 120 to the outside. In the cell treatment container 100 of the modification 14 of the seventh embodiment, the operator can identify the direction or position of the cell treatment container 100 based on the marking protrusion 381 by touching the outer peripheral surface 124 of the outer peripheral wall.
<Other preferred embodiment (2) regarding marking>
FIG. 39 shows a side view of the cell treatment container 100 of the present invention according to the modified example 15 of the seventh embodiment. The configuration of the other part of the cell treatment container 100 is as described in the seventh embodiment.

  The modification 15 of the seventh embodiment is an example in which markings 391 and 392 that can be visually identified are provided on the outer peripheral wall 120. The visually identifiable markings 391 and 392 are not particularly limited, and may be colored lines drawn on the surface of the outer peripheral wall 120, or visual marks included inside the resin outer peripheral wall 120. It may be a line such as a weld line that can be recognized by the user.

Although two markings 391 and 392 are provided in the illustrated example, the number is not particularly limited, and may be one, or two or three or more. When a plurality of markings are provided, by making one marking 391 and another marking 392 different in length and shape, it is possible to more easily identify the top, bottom, left and right of the cell treatment container 100.
<Other preferred embodiment (3) regarding marking>
In the cell treatment container 100 of the present invention, the marking may be provided on the container bottom 110. By providing the marking on the container bottom 110, the direction and position of the cell treatment container 100 can be easily grasped during microscopic observation.

FIG. 40A is a plan view of the cell treatment container 100 according to the modified example of the seventh embodiment of the present invention. The cell treatment container 100 of the modified example 16 of the seventh embodiment includes an identification line 401 on a part of the container bottom 110. FIG. 40B shows an end view of the EE end surface in FIG. 40A. The identification line 401 can be formed by slightly raising a part of the container bottom surface 111, which is the surface of the container bottom portion 110. Although not shown, the identification line 401 may be formed on the lower surface 112 of the container bottom 110.
<Other preferred embodiment (4) regarding marking>
FIG. 41A shows a plan view of the cell treatment container 100 of the present invention according to the modified example 17 of the seventh embodiment. The cell treatment container 100 of the modification 17 of the seventh embodiment includes identification circles 411 to 416 on a part of the container bottom 110. The identification circles 411 to 416 can be formed by slight steps on the container bottom surface 111 of the container bottom portion 110, as shown in FIG. 41B. Although not shown, the identification line 401 may be formed on the lower surface 112 of the container bottom 110. In the illustrated example, the plurality of identification circles 411 to 416 are arranged non-rotationally symmetrically, and the observer can easily identify the direction of the cell treatment container 100.

As shown in FIG. 41A, by arranging the identification circles 411, 412, 413, 414 of relatively large size and the identification circles 415, 416 of relatively small size on the container bottom 110 in a non-rotationally symmetric manner. It is preferable that the vertical and horizontal directions of the cell treatment container 100 can be more easily identified. Although not shown, three or more types of identification circles having different sizes and / or shapes may be arranged on the container bottom 110.
<Other preferred embodiment (5) regarding marking>
FIG. 42 is a plan view of the cell treatment container 100 of the present invention according to the modified example 18 of the seventh embodiment. The cell treatment container 100 of the modified example 18 of the seventh embodiment includes cross-shaped identification marks 421, 422, 423 on a part of the container bottom 110. The cross-shaped identification marks 421, 422, 423 can be formed, for example, on the container lower surface 112 of the container bottom 110 with a slight step. In the illustrated example, the plurality of identification marks 421, 422, 423 are arranged in a non-rotationally symmetric manner, so that the observer can easily identify the direction of the cell treatment container 100. Although not shown, two or more types of identification marks having different sizes and / or shapes may be arranged on the container bottom 110 so that the vertical and horizontal directions of the cell treatment container 100 can be more easily identified.
<Other preferred embodiment (6) regarding marking>
FIG. 43 is a plan view of the cell treatment container 100 of the present invention according to the modification 19 of the seventh embodiment. The cell treatment container 100 of the seventh modification of the seventh embodiment includes visually identifiable markings 431 and 432 on a part of the container bottom 110. The type of the markings 431, 432 is not particularly limited, and may be a colored line drawn on the surface of the container bottom 110, or a visually recognizable weld line included in the resin container bottom 110. May be a line. In the illustrated example, the plurality of markings 431 and 432 are arranged in a non-rotationally symmetric manner, so that the observer can easily identify the direction of the cell treatment container 100.

Although two markings 431 and 432 are provided in the illustrated example, the number is not particularly limited, and may be one or a plurality of two or three or more. When a plurality of markings are provided, by making one marking 431 and another marking 432 have different lengths and shapes, it is possible to more easily identify the top, bottom, left and right of the cell treatment container 100.
<Other preferred embodiment (7) regarding marking>
Multiple types of different markings can be used in combination.

  FIG. 44 is a plan view of the cell treatment container 100 of the present invention according to the modification 20 of the seventh embodiment. The cell treatment container 100 of Embodiment 7 Variant 20 is the cell treatment container according to Embodiment 7 Variant 13 (FIGS. 37A, 37B, 37C) or Embodiment 7 Variant 13 ′ in which a dent 371 is provided as one type of marking. On the container bottom 110 of 100, an identification line 401 similar to that of the seventh modified example 16 (FIGS. 40A and 40B) and identification circles 411 to 416 similar to that of the seventh modified example 17 (FIGs. 41A and 41B) are further added. It is an example of forming.

  By using a plurality of types of markings in combination in this way, it is possible to more easily identify the vertical and horizontal directions of the cell treatment container 100.

The container bottom 110 of the cell treatment container 100 according to the embodiment 7 Modification 13 ', and the identification line 401, the case of forming the identification circles 411 to 416, preferably identical, b, _{c 1} and _{c 2} and _{c 3} More preferably, b, c ₁ , c ₂ , c ₃ are smaller than a, and the outer peripheral wall upper end 122, the housing upper end 145, the first partition upper end 711, the second partition upper end 712, and the third partition upper end 713. The relative height relationship of is the same as in FIG. Further, preferably, d ₁ , d ₂ , d ₃ and e are the same, and the value thereof is not particularly limited, but 4 mm can be exemplified.
<Preferred embodiment (1) of combining micro tags>
A micro tag capable of further recording information and reading the recorded information with an appropriate reader can be attached to the cell treatment container 100 of the present invention. An RF (radio frequency) tag can be used as the micro tag. As the RF tag, a passive tag that does not have a built-in battery and can be downsized is preferable.

  In the cell treatment container 100 of the present invention having the outer lid portion 170 as shown in FIGS. 1C, 20, 22, and 33, and the cell treatment container 100 of the present invention having the inner lid portion 210 as shown in FIG. 21, The outer lid portion 170 or the inner lid portion 210 (hereinafter collectively referred to as “lid portion”) is provided with a first micro tag, and a container portion other than the lid portion of the cell treatment container 100 (hereinafter referred to as “container body portion”). It is preferable to provide a second micro tag in ().

When the cell treatment container 100 provided with a lid is used to treat cells derived from a specific patient or cells to be transplanted to a specific patient, examples of combinations of information recorded in the microtags can be exemplified below. .
(Aspect 1) An ID number (lid part ID number) for identifying the lid part is stored in the first micro tag of the lid part, and the container body part is identified in the second micro tag of the container body part. The ID number (container body section ID number) is stored. Separately, an ID number (patient ID number) for specifying a patient is set, and data in which a specific lid ID number, container body ID number, and patient ID number are associated with each other is created. The associated data may be separately recorded in a server or the like and used as a database. Further, as the lid ID number and the container body ID number, a unique ID originally held by the micro tag and different for each micro tag may be used, or a separate ID is issued and written in the micro tag. It may be stored.
(Aspect 2) An ID number (patient ID number) for identifying a patient is set, and the patient ID number is stored in each of the first micro tag of the lid and the second micro tag of the container body.

  In any of the embodiments, the lid portion and the container body portion of the cell treatment container 100 used for treating cells derived from a specific patient or cells to be transplanted to a specific patient are specified by reading the information stored in the microtag. Therefore, it is possible to prevent the containers from being mixed up with each other and to improve the traceability. In addition, a system that issues a warning when the lid and the container body are not intended for the same patient, and the patient ID number of the patient's chart is read and recorded in the first and / or second microtags. A system that reads information and issues a warning when there is a discrepancy, and reads the information recorded in the first and / or second micro tags at the beginning and end of each operation and records the information and time. It is possible to build various systems such as a system for improving traceability by leaving work history.

  The planar shape of the micro tag is not particularly limited, and may be circular (including a perfect circle, an ellipse, a flat perfect circle, a flat ellipse, etc.) or a rectangle, or may be another polygonal shape. Good.

  The frequency band of the RF tag is not particularly limited, and may be 13.56 MHz, 860 MHz to 960 MHz, or 2.45 GHz.

  The reading distance of the micro tag is not particularly limited. For example, when the micro tags are coupled to both the lid and the container body, the wireless output and antenna may be combined so that the information of both micro tags can be read at the same time. Communication may be performed only over a distance so that it can be confirmed that the cell treatment container 100 is reliably installed in a specific place.

  The micro tag may be bonded to both the lid and the container body, or may be bonded to only one of them. Since cells are contained in the container body, it is preferable to bind the microtag to at least the container body.

  A plurality of micro tags may be combined with at least one of the lid and the container body. When a plurality of tags are provided, the effect of expanding the communication range and the effect of being able to back up when one tag is broken can be expected.

The type of micro tag is not particularly limited. An example of a small micro tag is Muchip (trade name of Hitachi, Ltd.), which is an RF tag.
<Preferred embodiment (2) in which micro tags are combined>
The form in which the micro tag is bonded to the cell treatment container 100 of the present invention is not particularly limited, but it is preferable to bond the micro tag to a position that does not interfere with the work or a position that does not obstruct the visual field during observation.

  For example, FIG. 45 shows an example in which the micro tag 451 is bonded to the inner surface of the recessed portion 371 of the cell treatment container 100 of the present invention according to the thirteenth modified example of the seventh embodiment (FIGS. 37A, 37B, and 37C). Since the micro tag 451 is small, it can be housed in the recess 371 and does not interfere with the work. The method of binding the micro tag 451 to the cell treatment container 100 is not particularly limited, and may be joined with an adhesive or an adhesive tape, or may be embedded in a resin so that the surface of the cell treatment container 100 (in the example shown in the drawings, a hollow portion 371 inner surface). When the micro tag 451 is housed in the recess 371 and embedded by resin to be bonded, the recess 371 housing the micro tag 451 is completely filled with the resin so that the surface of the resin layer is the outer peripheral wall of the outer peripheral wall 120. The lower peripheral portion 124 ′ of the outer peripheral surface 124 may be matched, or the recessed portion 371 may be partially filled with resin to leave the recessed portion.

  Further, FIG. 46 shows an example in which the micro tag 461 is bonded onto the container bottom lower surface 113 of the container bottom 110 of the cell treatment container 100 of the present invention according to Modification 13 of Embodiment 7 (FIGS. 37A, 37B, 37C). . In this example, the container bottom lower surface 113 of the cell treatment container 100 is located in a recessed position rather than the container lower surface 112, and therefore, disposing the micro tag 461 on the container bottom lower surface 113 does not hinder the work.

When arranging the micro tag 461 on the container bottom 110 of the cell treatment container 100, it is preferable that it does not interfere with the observation when the operator observes it from above with a microscope or visually or from below with a microscope. For example the area of the plan view of the container bottom 111 is a container the inner surface of the container bottom 110 is 800 mm ² or more, for example 800mm ² ~2000mm ^2, if for example a 900mm ² ~1000mm ^2, preferably in the bottom of the container 110 is in plan view a circular In this case, the ratio of the area of the portion where the micro tag (including the chip and the antenna) overlaps with the container bottom surface 111 to the area of the container bottom surface 111 is preferably 5% or less, more preferably 1% or less, It is particularly preferably 0.1% or less. If the ratio is 5% or less, it is unlikely that the observation of cells on the bottom surface 111 of the container is blocked by the microtag, and the lower the ratio is, the lower the possibility is. When the overall shape of the bottom surface 111 of the container in a plan view is a circle with a diameter D ₁ , the ratio is 1% or less if the size of the microtag is within the range of a circle with a diameter of 0.1D ₁ or less. Become.
<Other preferred embodiment regarding shape of cell treatment container>
Each surface constituting the surface of the cell treatment container 100 of the present invention does not need to be a surface having a linear cross section, and may be a surface having a curved cross section. Further, each part of the cell treatment container 100 of the present invention may have a distorted shape.

  In FIG. 47, a cross-section corresponding to the XX cross section in FIG. 1A of a modification of the cell treatment container 100 of the present invention according to the first embodiment, in which the outer peripheral wall 120 is partially recessed radially inward. The schematic diagram of is shown. In this modified example, since the outer peripheral side surface 124 of the outer peripheral wall 120 has a recess, it is easy to hold by hand, which is preferable.

  FIG. 48 is a cross-sectional view corresponding to the XX cross section in FIG. 1A of a modified example of the cell treatment container 100 of the present invention according to the first embodiment in which the central portion of the container bottom portion 110 partially projects toward the inside of the container. The schematic diagram of is shown. In this modification, the container bottom surface 111 of the container bottom portion 110 sandwiched between the housing portion 130 and the outer peripheral wall portion 120 has the depth of the outer peripheral space 160 increased in the vicinity of the outer peripheral wall portion 120 (that is, approaches the container lower surface 112). Since it is formed as described above, cells on the container bottom surface 111 outside the housing portion 130 of the cell processing container 100 are easily gathered in the peripheral region of the container bottom surface 111 and cells are easily searched for, which is preferable.

  In FIG. 49, in the cell treatment container 100 of the present invention according to the first embodiment, the outer peripheral wall portion 120 is partially recessed inward in the radial direction, and the central portion of the container bottom portion 110 is partially protruded into the container. The schematic diagram of the cross section corresponded to the XX cross section in FIG. 1A of a modification is shown. The modification shown in FIG. 49 has the effects of both the modification shown in FIG. 47 and the modification shown in FIG.

<1. Manufacturing of containers>
<Production Example 1>
The cell treatment container 100 of Embodiment 1 having the shape shown in FIGS.

At the periphery of the container bottom 110, an inner peripheral contour is a circle having a diameter of 35 mm, and an outer peripheral wall 120 having a height from the container lower surface 112 to the outer peripheral wall upper end 122 of about 10 mm is provided. A cell treatment container 100 having 130 was produced. The housing portion forming surface 141 is a circle having a diameter of 13 mm in a plan view. The accommodation portion forming surface 141 includes an accommodation portion bottom surface 400 and an accommodation portion side surface 143 standing upright from the peripheral edge of the accommodation portion bottom surface 142. The height of the accommodation portion forming surface 141 from the deepest part 148 of the accommodation portion 130 forming surface 141 to the upper end 149 is 5.8 mm, and the height of the accommodation portion side surface 143 from the bottom surface side crossing portion 147 to the upper end 149 is 5 mm. And The accommodation portion bottom inclined surface 142 of the accommodation portion bottom surface 400 has an inverted cone shape with an inclination angle of 7 ° and is depressed so that the center of gravity of the accommodation portion bottom surface 400 in a plan view is the point-like deepest portion 148. The inclination angle of the housing portion side surface 143 was 85 °. The volume of the accommodation space 150 was about 660 μL. The deepest part 148 has a dot shape having substantially no width, and has a shape that falls within a range of 3 mm in diameter when the cell treatment container 100 is viewed in plan.
<Production Example 2>
2A and 2B, which have the same design as Manufacturing Example 1 except that the deepest part 148 corresponding to the apex of the inverted conical shape of the housing bottom surface 400 is eccentric from the center of gravity of the housing bottom surface 400 in plan view. The cell treatment container 100 of Embodiment 2 was produced.
<Production Example 3>
The cell treatment container 100 of Embodiment 3 having the shape shown in FIGS. 3A and 3B was produced.

At the periphery of the container bottom 110, an inner peripheral contour is a circle with a diameter of 35 mm, and an outer peripheral wall 120 having a height from the container lower surface 112 to the outer peripheral wall upper end 122 of about 10 mm is provided. A cell treatment container 100 having a housing portion 130 having a rectangular shape as viewed was produced. The accommodating portion forming surface 141 is a rectangle having a short side of 5 mm and a long side of 13 mm in plan view, the height from the deepest portion 148 to the upper end 149 of the accommodating portion forming surface 141 is 5.8 mm, and the accommodating portion side surface 143 is The height from the bottom side crossing portion 147 to the upper end 149 was 5 mm. The accommodating portion bottom inclined surface 142 has an inclination angle of 7 ° at the portion connected to the long side, an inclination angle of about 18 ° at the portion connected to the short side, and the center of gravity of the accommodation portion bottom surface 400 in the plan view is the deepest portion 148. As shown in FIG. The inclination angle of the housing portion side surface 143 was 85 °. The volume of the storage space 150 was about 325 μL. The deepest part 148 has a dot shape having substantially no width, and has a shape that falls within a range of 3 mm in diameter when the cell treatment container 100 is viewed in plan.
<Production Example 4>
A container similar to the cell treatment container of Production Example 1 was manufactured except that the height from the bottom side crossing portion 147 of the housing side surface 143 to the upper end 149 was 3 mm.
<Production Example 5>
A container similar to the cell treatment container of Production Example 1 was manufactured except that the height from the bottom side crossing portion 147 of the housing side surface 143 to the upper end 149 was 7 mm.
<2. Workability verification>
<Experiment 1>
The cell treatment containers of Examples 1 to 7 and Comparative Examples 1 and 2 shown in the table below were manufactured.

  The container of Example 4 is the container obtained in Production Example 1 above. The container of Example 3 is the container obtained in Production Example 4 above. The container of Example 5 is the container obtained in Production Example 5 above.

  The containers of the other examples and the comparative examples 1 and 2 have the same overall shape, the inclination angle of the accommodating portion bottom inclined surface 142, and the inclination angle of the accommodating portion side surface 143 as those of the container of the fourth embodiment. The height from the deepest portion 148 of the 141 to the upper end 149 (“formation surface height” in the table), and the height from the bottom surface side crossing portion 147 of the accommodation part side surface 143 to the upper end 149 (“side surface in the table”). “Height”), the diameter of the accommodation portion forming surface 141 in plan view (“diameter” in the table), and the capacity of the accommodation space 150 are the dimensions shown in the following table.

  The operability of the containers of Examples 1 to 7 and Comparative Examples 1 and 2 was evaluated.

  The accommodation space 150 of the accommodation unit 130 is filled with HTF medium (IRVINE SCIENTIFIC), glass beads (diameter about 150 μm) corresponding to the embryo are put, and a catheter (KITATATO ET catheter (catheter size 4Fr)) is accommodated. Whether the suction work by the catheter is easy by immersing in the space 150 (evaluation item 1), and the catheter tip collides with the peripheral wall portion 140 when performing the catheter work while observing under the microscope, or the microscope. It was evaluated whether or not there is a problem that it is difficult to work by hitting the lens (evaluation item 2).

  The results are shown in the "Catheter work" column of the table. Regarding the evaluation item 1 and the evaluation item 2, those having good workability are ○ (may be “A”), medium ones are Δ (may be “B”), and bad ones are x (“C”). If either one of the evaluation items 1 and 2 is x, the evaluation of “catheter work” is x (may be “C”), and there is no x in either of the evaluation items 1 and 2 and either or If both are Δ, the evaluation of “catheter work” is Δ (may be “B”), and if both evaluation items 1 and 2 are ○, the evaluation of “catheter work” is ○ (even if “A”) Good).

  None of the containers of Examples 1 to 7 had a "catheter work" of x. However, regarding the container of Example 1, the evaluation item 1 was Δ, and it was recognized that bubbles were likely to enter the catheter during the suction work with the catheter. In the container of Example 7, the evaluation item 2 is Δ, and the range in which the inclination of the catheter can be changed is limited, the work becomes difficult, and it tends to take time to suck the embryo and the like, and errors tend to increase.

  The container of Comparative Example 1 was evaluated as 1 for the evaluation item 1, and the problem of bubbles entering the catheter during the suction work with the catheter was remarkable.

  The container of Comparative Example 2 was “Poor” with respect to the evaluation item 2, and the range in which the inclination of the catheter could be changed was limited, which made the work difficult and took a lot of time to suck the embryo or the like, resulting in many errors.

When it was confirmed during the above-mentioned evaluation test whether the medium solution in the accommodation space 150 was volatilized, a slight amount of medium solution was confirmed to be volatilized in the containers of Examples 1 and 2. It was confirmed that the container of Comparative Example 2 had a large amount of volatile medium.
<Experiment 2>
The cell treatment containers of Examples 8 to 13 and Comparative Examples 3 and 4 shown in the table below were manufactured.

  The containers of Examples 8 to 13 and Comparative Examples 3 and 4 have the same overall shape, the inclination angle of the accommodation portion bottom inclined surface 142, and the inclination angle of the accommodation portion side surface 143 as those of the container of Manufacturing Example 3, and thus the accommodation portion is formed. The height from the deepest part 148 of the surface 141 to the upper end 149 (“formation surface height” in the table), and the height from the bottom surface side crossing portion 147 of the accommodation part side surface 143 to the upper end 149 (“in the table” The “side height”), the major axis length and the minor axis length of the accommodation portion forming surface 141 in plan view, and the capacity of the accommodation space 150 are the dimensions shown in the following table.

  The workability and direction recognizability of the containers of Examples 8 to 13 and Comparative Examples 3 and 4 were evaluated.

  The housing space 150 of the housing 130 is filled with HTF medium (IRVINE SCIENTIFIC), and a catheter (KITAZATO ET catheter (catheter size 4Fr)) is inserted from the minor axis side along the major axis direction of the opening of the housing 130. The workability (evaluation item 3) and the direction recognizability (evaluation item 4) when inserted into the accommodation space 150 and accessed in the center were confirmed.

  For each of the evaluation items 3 and 4, a good one is ◯ (“A” may be used), a medium grade is Δ (“B” may be used), and a bad one is X (“C” may be used). If either one of the evaluation items 3 and 4 is x, the evaluation of “catheter work” is x (may be “C”), and there is no x in either of the evaluation items 3 and 4 and one or both is If it is Δ, the evaluation of “catheter work” is Δ (may be “B”), and if both evaluation items 3 and 4 are o, the evaluation of “catheter work” is o (may be “A”). And The results are shown in the column "Catheter work" in the above table.

Among the containers of Examples 8 to 13, there was no container in which one or both of the evaluation items 3 and 4 were x. However, the container of Example 8 was slightly poor in workability because the width in the short axis direction was equal to or smaller than the width of the tip of the catheter, and was evaluated as Δ for the evaluation item 3. In the container of Example 13, the opening of the housing portion 130 is rectangular, but it is difficult to distinguish the major axis direction and the minor axis direction from the outside, and the minor axis length is relatively long, so the bank structure is within the visual field during microscopic observation. Is difficult to see, the direction recognizability is slightly poor, and the evaluation item 4 is Δ.
<Experiment 3>
In this experiment, the container of Example 4 (the container obtained in Production Example 1) was used.

  In the container of Comparative Example 5 used as a comparative example in the present experiment, the housing portion bottom surface 400 is formed by a flat plane whose peripheral edge has a circular shape in plan view and whose inclined surface is 0 °. The container has the same structure as the container of Example 4 except that the height of the side surface 143 is 5 mm. In the container of Comparative Example 5, the housing bottom surface 400 is formed by a circle having a diameter of 13 mm, and does not have a shape that fits within the range of 3 mm in diameter when the container is viewed in plan.

  One spherical glass bead with a diameter of 150 μm, HTF medium (manufactured by IRVINE SCIENTIFIC), and 400 μL were accommodated in the accommodation space of each container. After observing the contained glass beads with a microscope, the whole container was held, vibrated slightly, and allowed to stand. After standing, it was confirmed by a microscope whether the glass beads were in the initial arrangement position.

  In the container of Example 4, the glass beads were located in the cell holding region 146 including the deepest part 148 both before and after the vibration (evaluation ○). Here, the evaluation ◯ may be expressed as the evaluation A.

  In the container of Comparative Example 5, after the vibration, the glass beads largely moved from the position on the bottom surface before the vibration, and were located in the portion in contact with the side surface of the edge of the bottom surface, which made observation difficult (evaluation). X). Here, the evaluation x may be expressed as an evaluation C.

<Experiment 4>
The overall shape, the inclination angle of the accommodation portion bottom inclined surface 142, and the inclination angle of the accommodation portion side surface 143 are the same as those of the container of Example 4, and the height from the deepest portion 148 of the accommodation portion forming surface 141 to the upper end 149 is 4 mm. The prepared container was used as the cell treatment container 100 of Example 14. In this container, all θ ₄ are 32 ° and all θ ₅ are 27 °.

The container of Example 14 was placed on a horizontal stage, the housing space 150 of the housing unit 130 was filled with HTF medium (IRVINE SCIENTIFIC), and the cell holding area 146 was spherical glass with a diameter of about 150 μm corresponding to the embryo. Beads were added. While focusing on the glass beads and observing them with a stereoscopic microscope at a magnification of 20 times, an operation of sucking the glass beads from the tip of the catheter by about 4 mm was performed with a catheter (ET catheter made by KITAZATO (catheter size 4 Fr)). At this time, the acute angle of the angle formed by the length direction of the catheter and the plane along the mounting surface of the stage was set within the range of 60 ° to 40 °. As a result, when the acute angle is 60 ° to 50 °, there is a problem that the cells in the catheter are blurred and invisible, but if it is 45 ° or less, the cells can be visually recognized in the visual field, and if it is 40 ° or less. It became clear that there was no such problem.
<Experiment 5>
A stereomicroscope in which the cell treatment container 100 of Example 14 is placed on a horizontal stage and which is equipped with a cylindrical objective lens having a radius of 40 mm and whose optical axis is 20 × with the optical axis of the objective lens being perpendicular to the mounting surface of the stage. When the focus was set on the deepest portion 148, the distance between the objective lens and the deepest portion was 114 mm. Therefore, if both θ ₄ and θ ₅ are 81 ° or less in the largest part, the operation of bringing the catheter (KITAZATO ET catheter (catheter size 4Fr)) close to the deepest part 148 while observing the deepest part 148 in the microscope. At this time, it was revealed that the catheter did not interfere with the objective lens, the housing peripheral wall portion 140 and the outer peripheral wall portion 120. The lower limits of θ ₄ and θ ₅ are not particularly limited, and 1 ° is no problem.
<Experiment 6>
The cell treatment container 100 according to Embodiment 11 of the present invention shown in FIG. 11 was formed.

Heights d ₁ , d ₂ , d _{3 of} the first partition wall 701, the second partition wall 702, and the third partition wall 703 from the container bottom surface 111 (see FIG. 7A), and a height of the container peripheral wall portion 140 from the container bottom surface 111. All e (see FIG. 7A) were 4 mm. Three types of containers having diameters of 13 mm, 7 mm, and 3.8 mm at the upper end 149 of the formation surface of the accommodation space 150 were created.

  Surfaces inside the container, such as the forming surface 141 of the cell treatment container 100, the side wall surface surrounding the second container-shaped portion accommodating space 162, and the container bottom surface 111, were all hydrophobic surfaces.

  In this cell treatment container 100, one surface 707 of the third partition wall 703 and the housing outer wall surface 144 of the housing peripheral wall portion 140, one surface 707 of the third partition wall 703, and the outer peripheral wall portion inner peripheral surface 121 of the outer peripheral wall portion 120. Are directly intersected with each other and are not curved.

  When oil was added to the second container-shaped portion accommodation space 162 of the cell treatment container 100 to a depth of 0.5 mm, one surface 707 of the third partition wall 703 and the inner circumference of the outer peripheral wall portion of the outer peripheral wall portion 120. The oil diffused along the portion where the surface 121 intersects and flowed into the third container-shaped portion accommodation space 163.

  On the other hand, oil was added to the accommodation space 150 of the accommodation portion 130 of the cell treatment container 100 such that the liquid surface was positioned 0.5 mm below the upper end 149 of the forming surface 141, which is the upper end of the forming surface 141. The oil remained in the accommodation space 150 without exceeding the upper end 149 of the forming surface regardless of the case where the diameter of the upper end 149 of the forming surface was 13 mm, 7 mm, or 3.8 mm.

From this, as shown in FIG. 31, one surface 707 of the third partition wall 703 and the housing outer wall surface 144 of the housing peripheral wall portion 140, one surface 707 of the third partition wall 703 and the outer peripheral wall of the outer peripheral wall portion 120. If the inner peripheral surface 121 of the part is smoothly connected by the rounded first partition wall side-accommodating part outer wall connection curved surface 311 and the first partition wall side-outer peripheral wall inner peripheral surface connection curved surface 313, the second container shape is obtained. It has been clarified that the oil can be prevented from diffusing from the part accommodation space 162 to the third container-shaped part accommodation space 163.
<Experiment 7>
In cell culture, a culture solution may be formed into a drop shape on a substrate, and the drop may be covered with oil or the like to prevent evaporation of the culture solution, and cells may be cultured in the drop.

  When fertilized eggs are cultured in drops of culture medium, the volume of the culture medium forming the drops is usually 15 μL or more.

  Therefore, 15 μL of a cell culture solution, which is an aqueous solution in which each component is dissolved in water, is placed on the surface of a hydrophobic polystyrene base material that has not been subjected to a hydrophilic treatment to form a drop, and the drop is removed from the surface of the base material. I confirmed the height. As a result, it was confirmed that the height of the drop was 2 mm. A layer of oil deeper than 2 mm is required to cover the drop.

100 .. Cell treatment container, 110 .. container bottom, 120 .. outer peripheral wall part, 130 .. accommodation part, 150 .. accommodation space of accommodation part, 141 .. forming surface, 142 ... 148 .. deepest part, 146 .. cell retention region All publications, patents and patent applications cited herein are incorporated herein by reference in their entirety.

Claims (3)

The bottom of the container,
An outer peripheral wall portion standing up from the peripheral edge of the container bottom,
A cell treatment container provided with one or more accommodating portions that are provided at the bottom of the container and in which an accommodation space for accommodating cells and a cell treatment liquid is formed and open upward,
The volume of the accommodation space is 200 μl or more and 1000 μl or less,
The formation surface, which is a surface that forms the accommodation space, of the accommodation portion includes a deepest portion that is a deepest portion of the accommodation portion and an inclined surface that is formed so as to surround the deepest portion. Have,
The inclined surface is continuous with the deepest part,
The deepest part is formed to fit within the circle of diameter 3mm in a plan view of the cell treatment container,
The container further comprises two or more partition walls that stand upright from the bottom of the container and bridge the storage portion and the outer peripheral wall portion,
A container-shaped part accommodation space for accommodating cells and / or liquid, which is surrounded by the container part, the outer peripheral wall part, and an adjacent pair of the two or more partition walls, with the container bottom part as a bottom part. One or more container-shaped portions formed with
The cell treatment container. The upper end of the accommodating portion and the upper end of each of the two or more partition walls are independently located at the same vertical position as the upper end of the outer peripheral wall portion, or below the upper end of the outer peripheral wall portion. The cell treatment container according to claim 1, wherein: The cell treatment container according to claim 2, wherein the upper end of the outer peripheral wall is located above the upper end of the accommodation part and the upper ends of the two or more partition walls.

Images