CN211712992U

CN211712992U - A extraction element for preparing monoclonal antibody

Info

Publication number: CN211712992U
Application number: CN201922312215.8U
Authority: CN
Inventors: 闫乐乐; 王坤; 黎四平
Original assignee: Dongguan Eighth People's Hospital (dongguan Children's Hospital); Qingdao Jiuning Pharmaceutical Technology Co ltd; Tianjin Protech Biotechnology Co ltd
Current assignee: Dongguan Eighth People's Hospital (dongguan Children's Hospital); Qingdao Jiuning Pharmaceutical Technology Co ltd; Tianjin Protech Biotechnology Co ltd
Priority date: 2019-12-20
Filing date: 2019-12-20
Publication date: 2020-10-20
Anticipated expiration: 2029-12-20

Abstract

The utility model discloses an extraction element for preparing monoclonal antibody, including workstation and supporting leg, the welding of center department of workstation top outer wall has the protecting crust, the joint has axial contact bearing on the bottom inner wall of protecting crust, and the inside of protecting crust rotates through axial contact bearing and is connected with the centrifuge bowl, there is the motor one side of workstation top outer wall through bolted connection, the output of motor runs through to the below of workstation, and the key connection has driving pulley on the output of motor, it is connected with the main shaft to rotate through the bearing on the bottom outer wall of workstation, the one end joint of main shaft is on the bottom outer wall of centrifuge bowl, and the other end key-type connection of main shaft has driven pulley. The utility model discloses a main shaft passes through the structural connection of fixed block and fixed slot, does not restrict centrifugal cylinder and removes on the axial direction when can transmit the moment of torsion in radial direction, and the structure is dismantled conveniently, only needs to mention centrifugal cylinder can separate centrifugal cylinder and main part, has reduced the maintenance cost.

Description

A extraction element for preparing monoclonal antibody

Technical Field

The utility model relates to a bioengineering technical field especially relates to an extraction element for preparing monoclonal antibody.

Background

Monoclonal antibodies are highly homogeneous antibodies produced by a single B cell clone, directed only to a specific epitope, and are generally prepared by the hybridoma technique, which is a fusion of a sensitized B cell having the ability to secrete a specific antibody and a myeloma cell having an unlimited proliferation ability into a B cell hybridoma based on a cell fusion technique.

However, the transmission mechanism and the centrifuge bowl of the general device of the existing monoclonal antibody extraction device are of an integrated structure, and the whole device needs to be completely disassembled during maintenance, so that the maintenance work is difficult, and the maintenance cost is increased. Therefore, it is desirable to design an extraction apparatus for preparing monoclonal antibodies to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects in the prior art and providing an extraction device for preparing monoclonal antibodies.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides an extraction element for preparing monoclonal antibody, includes workstation and supporting leg, the welding of the center department of workstation top outer wall has the protecting crust, the joint has axial contact bearing on the bottom inner wall of protecting crust, and the inside of protecting crust rotates through axial contact bearing and is connected with the centrifuge bowl, there is the motor one side of workstation top outer wall through bolted connection, the output of motor runs through to the below of workstation, and the key joint has driving pulley on the output of motor, rotate through the bearing on the bottom outer wall of workstation and be connected with the main shaft, the one end joint of main shaft is on the bottom outer wall of centrifuge bowl, and the other end key-type connection of main shaft has driven band pulley, driven band pulley passes through the belt and is connected with the driving pulley transmission.

Further, be connected with the top cap through hinge rotation on the top outer wall of protecting crust, and peg graft on the top outer wall of top cap and have discharging pipe and the wasting discharge pipe that is horizontal structure and distributes, discharging pipe and wasting discharge pipe all link up to the inside of centrifuge bowl, and the one end threaded connection of discharging pipe has the filter tube.

Furthermore, a clamping groove is formed in the inner wall of one side of the filter pipe, and the inside of the filter pipe is connected with a precise filter element through the clamping groove in a clamping mode.

Furthermore, a fixing groove is formed in the outer wall of the bottom of the centrifugal cylinder, a fixing block is welded to the outer wall of the side face of the main shaft, and the main shaft is clamped inside the fixing groove through the fixing block.

Furthermore, the welding has the equidistance to become rectangle structure distribution spliced pole on the bottom outer wall of workstation, and the welding has the guide post on the bottom outer wall of spliced pole, the guide post plug-in is in the inside of supporting leg, and cup joints the spring on the side outer wall of guide post.

Furthermore, a baffle is welded on the outer wall of the top of the centrifugal cylinder, and a feeding port is formed in the center of the outer wall of the top of the baffle.

The utility model has the advantages that:

1. through the main shaft and the centrifugal cylinder that set up, the main shaft passes through the structural connection of fixed block and fixed slot, does not restrict the centrifugal cylinder and removes in the axial direction when can transmit the moment of torsion in radial direction, and the bottom of centrifugal cylinder is connected with the workstation through the axial contact bearing for bear the gravity of centrifugal cylinder itself, make the structure dismantle convenient, only need mention the centrifugal cylinder can separate centrifugal cylinder and main part, make the dismantlement process of maintaining simplify greatly, reduced the maintenance cost.

2. Through the filter tube that sets up, the direct threaded connection of filter tube is in the one end of discharging pipe, and the in-process of the ejection of compact is direct to be filtered the extract, has saved the time, has improved extraction efficiency, and filterable precision filter core joint is in the inside of filter tube simultaneously, and the split of filter tube and filter core is also very simple and convenient for the clearance becomes convenient.

3. Through the supporting leg that sets up, the supporting leg is connected with the spliced pole through the spring, and the spring can be kept apart the vibrations on ground, prevents to receive the impact at the device during operation and causes the unstable condition emergence of centrifuge bowl work, has reduced the unexpected condition emergence probability in the production process.

Drawings

FIG. 1 is a schematic structural diagram of an extraction apparatus for preparing monoclonal antibodies according to the present invention;

FIG. 2 is a sectional view of the structure of an extraction apparatus for preparing monoclonal antibodies according to the present invention;

FIG. 3 is a schematic diagram showing a partial enlarged structure A of an extraction apparatus for preparing monoclonal antibodies according to the present invention;

fig. 4 is a schematic view of a cross-sectional structure of a filter tube of an extraction device for preparing monoclonal antibodies according to the present invention.

In the figure: 1 workbench, 2 protective shells, 3 top covers, 4 discharge pipes, 5 filter pipes, 6 waste discharge pipes, 7 motors, 8 clamping grooves, 9 precise filter elements, 10 connecting columns, 11 guide columns, 12 springs, 13 supporting legs, 14 driving belt wheels, 15 driven belt wheels, 16 main shafts, 17 centrifugal cylinders, 18 baffles, 19 fixing blocks, 20 fixing grooves and 21 axial contact bearings.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1-4, an extraction apparatus for preparing monoclonal antibody comprises a workbench 1 and support legs 13, a protective shell 2 welded at the center of the top outer wall of the workbench 1 for protecting an internal centrifugal cylinder 17, an axial contact bearing 21 clamped on the bottom inner wall of the protective shell 2 for bearing the axial force of the centrifugal cylinder 17, and the protective shell 2 is rotatably connected with the centrifugal cylinder 17 through the axial contact bearing 21 for separating the components in the raw materials by rotation, a motor 7 connected with one side of the top outer wall of the workbench 1 through a bolt, the motor 7 being of model NV420EAI, the output end of the motor 7 penetrating to the lower part of the workbench 1, and the output end of the motor 7 being key-connected with a driving pulley 14, a main shaft 16 rotatably connected with the bottom outer wall of the workbench 1 through a bearing, one end of the main shaft 16 being clamped on the bottom outer wall of the centrifugal cylinder 17, and the other end of the main shaft 16 being key-connected with a driven pulley 15, the driven pulley 15 is in transmission connection with the driving pulley 14 through a belt and is used for transmission of the device.

Further, be connected with top cap 3 through hinge rotation on the top outer wall of protecting crust 2, and peg graft on the top outer wall of top cap 3 and have discharging pipe 4 and the exhaust waste pipe 6 that is the horizontal structure and distributes, discharging pipe 4 and exhaust waste pipe 6 all link up to the inside of centrifuge tube 17, and a terminal threaded connection of discharging pipe 4 has filter tube 5, directly filters the extract at the in-process of the ejection of compact, has saved the time.

Furthermore, a clamping groove 8 is formed in the inner wall of one side of the filter pipe 5, and a precision filter element 9 is clamped in the filter pipe 5 through the clamping groove 8 to filter the components of the discharged materials.

Furthermore, a fixing groove 20 is formed in the outer wall of the bottom of the centrifugal cylinder 17, a fixing block 19 is welded to the outer wall of the side face of the main shaft 16, and the main shaft 16 is clamped inside the fixing groove 20 through the fixing block 19 and used for transmission between the main shaft 16 and the centrifugal cylinder 17.

Further, the welding has the equidistance to become rectangle structure distribution spliced pole 10 on the bottom outer wall of workstation 1, and the welding has guide post 11 on the bottom outer wall of spliced pole 10, and guide post 11 pegs graft in the inside of supporting leg 13, and has cup jointed spring 12 on the side outer wall of guide post 11, can keep apart the vibrations on ground, prevents to receive the circumstances that the impact caused centrifuge bowl 17 unstable work to take place when the device is worked.

Furthermore, a baffle 18 is welded on the outer wall of the top of the centrifugal cylinder 17 to prevent raw materials from splashing, and a feed inlet is formed in the center of the outer wall of the top of the baffle 18 to facilitate the addition of raw materials into the centrifugal cylinder 17.

The working principle is as follows: when the centrifugal machine is used, an operator firstly opens the top cover 3 through a hinge, then places the clean centrifugal cylinder 17 in the protective shell 2, enables the fixing block 19 of the main shaft 16 to be clamped in the fixing groove 20, then adds raw materials into the centrifugal cylinder 17 through the feeding port, then covers the top cover 3, connects the filter pipe 5 on the discharging pipe 4 in a threaded manner, and connects the discharging pump and the waste discharging pump on the filter pipe 5 and the waste discharging pipe 6 respectively; when the centrifugal machine works, an operator firstly turns on the motor 7, the motor 7 drives the driving belt wheel 14 which is in key connection with the main shaft of the motor 7 to rotate, the driving belt wheel 14 drives the driven belt wheel 15 to rotate through a belt, the driven belt wheel 15 drives the main shaft 16 to rotate, the main shaft 16 drives the centrifugal cylinder 17 to rotate through the fixing block 19 and the fixing groove 20, the centrifugal cylinder 17 drives the raw materials inside the centrifugal cylinder 17 to rotate quickly, different components are separated from each other due to different deadweights of the different components inside the raw materials, heavier components are distributed on the periphery, lighter components are distributed on the periphery, after the centrifugal machine is centrifuged for a period of time, the operator can close the motor 7, the heavier components are arranged on the lower part at the moment, lighter components are arranged on the upper part, the upper components are sucked out through the discharge pipe 4 and are collected through the filtration of the precision.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides an extraction element for preparing monoclonal antibody, includes workstation (1) and supporting leg (13), its characterized in that, the welding of the center department of workstation (1) top outer wall has protecting crust (2), the joint has axial contact bearing (21) on the bottom inner wall of protecting crust (2), and the inside of protecting crust (2) rotates through axial contact bearing (21) and is connected with centrifuge tube (17), there is motor (7) one side of workstation (1) top outer wall through bolted connection, the output of motor (7) runs through to the below of workstation (1), and key connection has driving pulley (14) on the output of motor (7), it is connected with main shaft (16) through the bearing rotation on the bottom outer wall of workstation (1), the one end joint of main shaft (16) is on the bottom outer wall of centrifuge tube (17), and the other end key of the main shaft (16) is connected with a driven belt wheel (15), and the driven belt wheel (15) is in transmission connection with the driving belt wheel (14) through a belt.

2. The extraction device for preparing monoclonal antibodies, according to claim 1, characterized in that the top outer wall of the protective shell (2) is rotatably connected with a top cover (3) through a hinge, and the top outer wall of the top cover (3) is inserted with a discharge pipe (4) and a waste discharge pipe (6) which are distributed in a horizontal structure, the discharge pipe (4) and the waste discharge pipe (6) both penetrate through the interior of the centrifuge tube (17), and one end of the discharge pipe (4) is in threaded connection with the filter pipe (5).

3. The extraction device for preparing monoclonal antibodies of claim 2, wherein the inner wall of one side of the filter tube (5) is provided with a clamping groove (8), and the inside of the filter tube (5) is clamped with a precision filter element (9) through the clamping groove (8).

4. The extraction device for preparing monoclonal antibodies, according to claim 1, characterized in that the bottom outer wall of the centrifuge bowl (17) is provided with a fixing groove (20), the side outer wall of the main shaft (16) is welded with a fixing block (19), and the main shaft (16) is clamped inside the fixing groove (20) through the fixing block (19).

5. The extraction device for preparing monoclonal antibodies, according to claim 1, characterized in that the outer wall of the bottom of the workbench (1) is welded with connecting columns (10) distributed in a rectangular structure at equal intervals, the outer wall of the bottom of the connecting columns (10) is welded with guide columns (11), the guide columns (11) are inserted into the supporting legs (13), and the outer wall of the side surfaces of the guide columns (11) is sleeved with springs (12).

6. The extraction apparatus for preparing monoclonal antibodies according to claim 1, wherein the top outer wall of the centrifuge cylinder (17) is welded with a baffle (18), and the center of the top outer wall of the baffle (18) is provided with a feeding port.