CN211402399U - Enzyme-linked immunoassay instrument for detecting beta-receptor stimulant - Google Patents
Enzyme-linked immunoassay instrument for detecting beta-receptor stimulant Download PDFInfo
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- CN211402399U CN211402399U CN201921363041.1U CN201921363041U CN211402399U CN 211402399 U CN211402399 U CN 211402399U CN 201921363041 U CN201921363041 U CN 201921363041U CN 211402399 U CN211402399 U CN 211402399U
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Abstract
The utility model relates to a special instrument for enzyme-linked immunosorbent assay, in particular to an enzyme-linked immunosorbent assay instrument for detecting beta-receptor stimulant. The utility model discloses design a full automatization ELISA detector on traditional manual operation's basis, manual operation's trouble has been saved, specifically for installing rotatory liquid suction device on examining test table, accomplish the absorption to the diluent through the pipette on the rotatory lotion device, thereby instil into on the incubation joins in marriage liquid washing device's the cell, the bottom of cell is the self-heating base, join in marriage liquid washing device at the incubation and be equipped with the blowing device respectively on the corresponding position with the cell all around, first fixed dropping liquid device, the fixed dropping liquid device of light source device and second, through the very big trouble of having saved human operation of above device, the precision of detection has been improved simultaneously.
Description
Technical Field
The utility model relates to a special instrument for enzyme-linked immunosorbent assay, in particular to an enzyme-linked immunosorbent assay instrument for detecting beta-receptor stimulant.
Background
The special instrument for enzyme-linked immunosorbent assay can be divided into 2 categories of semi-automatic and full-automatic ones, but its working principle is basically identical, and its core is a colorimeter, i.e. it uses colorimetry to analyze antigen or antibody content. The ELISA measurement generally requires that the final volume of the test solution is below 250u l, and the test cannot be completed by using a general photoelectric colorimeter, so that special requirements are imposed on the photoelectric colorimeter in the microplate reader. The steps of the enzyme-linked immunoassay process are complex, the traditional manual operation wastes time and labor, the detection efficiency is extremely low, and the problem of low efficiency of single manual operation can be solved by designing the enzyme-linked immunoassay instrument for detecting the beta-receptor stimulant.
SUMMERY OF THE UTILITY MODEL
The technical problem to be solved by the utility model is to provide an enzyme-linked immunoassay instrument for detecting beta-receptor stimulant aiming at the technical defects, the utility model designs a full-automatic enzyme-linked immunoassay instrument based on the traditional manual operation, which saves the trouble of manual operation, in particular to install a rotary imbibing device on a detection table, and the absorption of diluent is completed by a pipette on a rotary washing liquid device, thereby dropping the solution on a cuvette of the incubation solution preparation washing device, the bottom end of the cuvette is an automatic heating base, a blowing device, a first fixed dropping device, a light source device and a second fixed dropping device are respectively arranged at the corresponding positions of the periphery of the incubation liquid preparation washing device and the cuvette, through the device, the trouble of manual operation is greatly saved, and the detection precision is improved.
In order to solve the technical problem, the utility model discloses the technical scheme who adopts is: an enzyme-linked immunoassay instrument for detecting a beta-receptor stimulant is characterized by comprising a detection table, wherein a rotary imbibing device is arranged on the detection table, an incubation liquid preparation washing device is arranged on one side of the rotary imbibing device, the incubation liquid preparation washing device comprises a rotary workbench and a plurality of heating bases which are circumferentially distributed on the rotary workbench, a cuvette with a movable bottom surface is placed on the heating bases, an electric telescopic rod is arranged in each heating base, and a telescopic part of each electric telescopic rod is connected with the movable bottom surface; a blowing device is arranged at the position, corresponding to the cuvette, on the periphery of the outer side of the rotary worktable; and a first fixed dropping device, a light source device for projecting light and a second fixed dropping device corresponding to the cuvette are arranged around the outside of the incubation liquid preparation washing device.
Further optimize this technical scheme, be equipped with the display screen on the test table.
Further optimize this technical scheme, it holds the ware to install on the test table, it is connected with the fluid passage to hold the ware.
Further optimize this technical scheme, install a plurality of pipettes on the rotatory imbibition device.
Further optimize this technical scheme, the blowing device is including blowing the tuber pipe.
Further optimize this technical scheme, be equipped with the switch gate on the detection platform.
Further optimize this technical scheme, the material of cell is polyvinyl chloride.
Further optimize this technical scheme, it is equipped with a plurality of heels and gyro wheel to examine test table bottom.
Compared with the prior art, the utility model has the advantages of it is following: 1. the utility model adopts the structural design of the rotary imbibition device, and the structure ensures that the beta-receptor stimulant flowing from the containing vessel to the fluid passage can be continuously absorbed, thereby improving the detection efficiency; 2. the utility model adopts the structural design of the cuvette with the movable bottom surface, and the structure ensures that the waste liquid after washing can be discharged from the cuvette by controlling the movable bottom surface through the telescopic part of the electric telescopic rod; 3. the utility model discloses a structural design of the fixed dropping liquid device of blowing device, first fixed dropping liquid device, light source device and second, artifical repetitive operation has been avoided to this structure, has improved the precision that detects simultaneously.
Drawings
FIG. 1 is a schematic diagram of the overall structure of an ELISA detector for detecting β -receptor agonists.
FIG. 2 is a schematic diagram of a portion of an ELISA detector for detecting β -receptor agonists.
FIG. 3 is a schematic diagram of a test structure of an ELISA detector for detecting β -receptor agonists.
FIG. 4 is a schematic view of a heating base of an ELISA detector for detecting β -receptor agonists.
In the figure: 1. a detection table; 2. rotating the liquid suction device; 3. an incubation liquid preparation washing device; 4. rotating the working table; 5. heating the base; 6. a movable bottom surface; 7. a cuvette; 8. an electric telescopic rod; 9. a telescopic part; 10. A blowing device; 11. a first fixed dripping device; 12. a light source device; 13. a second fixed dripping device; 14. a display screen; 15. a containing vessel; 16. a fluid passage; 17. a pipette; 18. blowing an air pipe; 19. Opening and closing a gate; 20. a bottom pad; 21. and a roller.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings. It should be understood that the description is intended to be illustrative only and is not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
The specific implementation mode is as follows: referring to FIGS. 1-4, FIG. 1 is a schematic diagram of the overall structure of an ELISA detector for detecting β -receptor agonists; FIG. 2 is a schematic diagram of a portion of an ELISA detector for detecting β -receptor agonists; FIG. 3 is a schematic diagram of a test configuration of an ELISA detector for detecting β -receptor agonists; FIG. 4 is a schematic view of a heating base of an ELISA detector for detecting β -receptor agonists.
The utility model provides an enzyme-linked immunosorbent assay instrument for detecting beta-receptor stimulant, which is characterized in that the enzyme-linked immunosorbent assay instrument comprises a detection table 1, wherein a rotary imbibition device 2 is arranged on the detection table 1, one side of the rotary imbibition device 2 is provided with an incubation liquid preparation washing device 3, the incubation liquid preparation washing device 3 comprises a rotary workbench 4 and a plurality of heating bases 5 which are circumferentially distributed on the rotary workbench 4, a cuvette 7 with a movable bottom surface 6 is placed on the heating bases 5, an electric telescopic rod 8 is arranged inside the heating bases 5, and a telescopic part 9 of the electric telescopic rod 8 is connected with the movable bottom surface 6; a blowing device 10 is arranged at the position, corresponding to the cuvette 7, on the periphery of the outer side of the rotary worktable 4; the periphery of the outside of the incubation liquid preparation washing device 3 is provided with a first fixed dropping device 11 corresponding to the cuvette 7, a light source device 12 for projecting light and a second fixed dropping device 13.
Further optimizing the technical scheme, the detection platform 1 is provided with a display screen 14.
Further optimize this technical scheme, install on the detection platform 1 and hold ware 15, hold ware 15 and be connected with fluid passage 16.
Further optimizing the technical scheme, a plurality of liquid suction pipes 17 are arranged on the rotary washing and sucking device 2.
Further optimizing the technical solution, the blowing device 10 includes a blowing pipe 18.
Further optimizing the technical scheme, the detection platform 1 is provided with a switch gate 19.
Further optimize this technical scheme, the material of cell 7 is polyvinyl chloride.
Further optimize this technical scheme, it is equipped with a plurality of heelpieces 20 and gyro wheel 21 to examine test table 1 bottom.
In the above embodiment, the present invention adopts the structural design of the rotary imbibing device 2, which ensures that the β -receptor stimulant flowing from the vessel 15 to the fluid passage 16 can be continuously imbibed, thereby improving the detection efficiency; the utility model adopts the structural design of the cuvette 7 with the movable bottom surface 6, and the structure ensures that the waste liquid after washing can be discharged out of the cuvette 7 by controlling the movable bottom surface 6 through the telescopic part 9 of the electric telescopic rod 8; the utility model discloses a structural design of blowing device 10, the fixed dropping liquid device of first 11, light source device 12 and the fixed dropping liquid device of second 13, artifical repetitive operation has been avoided to this structure, has improved the precision that detects simultaneously.
To facilitate understanding of the present invention, an enzyme-linked immunoassay device for detecting β -receptor agonists is provided, and is described in detail below with reference to specific examples.
An enzyme-linked immunoassay instrument for detecting beta-receptor stimulant comprises a detection table 1, a display screen 14 is arranged on the detection table 1, a containing vessel 15 and a fluid passage 16 connected with the containing vessel 15 are arranged opposite to the display screen 14, a rotary liquid suction device 2 is arranged at the other end of the fluid passage 16, a liquid suction pipe 17 is arranged on the rotary liquid suction device 2, the liquid suction pipe 17 can suck liquid flowing into the fluid passage 16 from the containing vessel 15, the process of uninterrupted liquid suction and continuous dripping can be achieved through the rotary liquid suction device 2, one side of the rotary liquid suction device 2 is an incubation liquid preparation washing device 3, the incubation liquid preparation washing device 3 comprises a rotary workbench 4 and a plurality of heating bases 5 positioned on the rotary workbench 4, each heating base 5 is respectively provided with a cuvette 7 with a movable bottom surface 6, and particularly the cuvette 7 is made of polyvinyl chloride; an electric telescopic rod 8 is arranged in the heating base 5, and a telescopic part 9 of the electric telescopic rod 8 is connected with the movable bottom surface 6 of the cuvette 7; the periphery of the outer side of the rotary workbench 4 is provided with a blowing device 10 corresponding to the cuvette 7, the blowing device 10 blows air into the cuvette 7 through a blowing air pipe 18, the periphery of the outer side of the rotary workbench 4 is also provided with a light source device 12 for lighting, a first fixed liquid dropping device 11 and a second fixed liquid dropping device 13 for adding enzyme, water and colorant, and the bottommost end of the detection platform 1 is provided with a plurality of bottom pads 20 and rollers 21;
when in use: firstly, putting a beta-receptor stimulant into a containing vessel 15, enabling the beta-receptor stimulant to pass through a fluid passage 16, starting a rotary imbibing device 2 to absorb the beta-receptor stimulant in the fluid passage 16 through a pipette 17, then, rotatably dripping the beta-receptor stimulant into a cuvette 7 of a rotary workbench 4, sealing the cuvette 7 by a film, starting a heating base 5 to heat the beta-receptor stimulant in the cuvette 7, setting the temperature to be about 37 ℃ and incubating for about 30 minutes, then, starting the rotary workbench 4 and a first fixed dripping device 11, carefully uncovering the film and closing the heating base 5;
secondly, dripping distilled water into the cuvette 7, starting the electric telescopic rod 8 after standing for about 30s, shortening the telescopic part 9 of the electric telescopic rod 8, driving the movable bottom surface 6 of the cuvette 7 to move downwards, enabling the wastewater to flow out of the cuvette 7, driving the telescopic part 9 to restore by the electric telescopic rod 8, and restoring the movable bottom surface 6 to the original state;
step three, opening the blowing device 10, blowing air into the cuvette through the blowing air pipe 18 to air the residual liquid in the cuvette 7, repeating the operation of the step two, namely filling distilled water into the cuvette 7, standing for 30s, lowering the movable bottom surface 6 by using the electric telescopic rod 8, allowing the wastewater to flow out of the cuvette 7, and opening the blowing device 10 to air-dry the residual liquid in the cuvette 7;
step four, after the washing process of the step one and the step two is repeated for a plurality of times, the movable bottom surface 6 is in a normal position, at this time, a certain amount of enzyme-labeled reagent is dripped into the cuvette 7 through the first fixed dripping device 11, then the cuvette 7 is sealed by a membrane, the heating base 5 is started again, the same set temperature is about 37 ℃, and the incubation time is not less than 30 minutes;
fifthly, carefully uncovering the film, controlling the electric telescopic rod 8 to enable the movable bottom surface 6 to move downwards, enabling the wastewater to leave the cuvette 7, then reducing the movable bottom surface 6, and repeating the washing process for 3-5 times;
step six, when the movable bottom surface 6 is in a normal position, the second fixed dropping device 13 is started to drop the colorant into the cuvette 7, then the cuvette 7 is irradiated by the light source device 12, the photoelectric detector converts the light energy into an electric signal to appear on the display screen 14, and thus the whole process of enzyme-linked immunoassay is completed.
The utility model adopts the structural design of the rotary imbibing device 2, which ensures that the beta-receptor stimulant flowing from the containing vessel 15 to the fluid passage 16 can be continuously imbibed, thereby improving the detection efficiency;
the utility model adopts the structural design of the cuvette 7 with the movable bottom surface 6, and the structure ensures that the waste liquid after washing can be discharged out of the cuvette 7 by controlling the movable bottom surface 6 through the telescopic part 9 of the electric telescopic rod 8;
the utility model discloses a structural design of blowing device 10, the fixed dropping liquid device of first 11, light source device 12 and the fixed dropping liquid device of second 13, artifical repetitive operation has been avoided to this structure, has improved the precision that detects simultaneously.
It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explaining the principles of the invention and are not to be construed as limiting the invention. Therefore, any modification, equivalent replacement, improvement and the like made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. Further, it is intended that the appended claims cover all such variations and modifications as fall within the scope and boundaries of the appended claims or the equivalents of such scope and boundaries.
Claims (8)
1. The enzyme-linked immunoassay instrument for detecting the beta-receptor stimulant is characterized by comprising a detection table (1), wherein a rotary imbibing device (2) is arranged on the detection table (1), an incubation liquid preparation washing device (3) is arranged on one side of the rotary imbibing device (2), the incubation liquid preparation washing device (3) comprises a rotary workbench (4) and a plurality of heating bases (5) which are circumferentially distributed on the rotary workbench (4), a cuvette (7) with a movable bottom surface (6) is placed on the heating bases (5), an electric telescopic rod (8) is arranged inside the heating bases (5), and a telescopic part (9) of the electric telescopic rod (8) is connected with the movable bottom surface (6); a blowing device (10) is arranged at the position, corresponding to the cuvette (7), on the periphery of the outer side of the rotary worktable (4); and a first fixed dropping device (11), a light source device (12) for projecting light and a second fixed dropping device (13) which correspond to the cuvette (7) are arranged around the outside of the incubation liquid preparation washing device (3).
2. The ELISA detector of claim 1, wherein the detection signal comprises at least one of: a display screen (14) is arranged on the detection table (1).
3. The ELISA detector of claim 1, wherein the detection signal comprises at least one of: the detection table (1) is provided with a containing vessel (15), and the containing vessel (15) is connected with a fluid passage (16).
4. The ELISA detector of claim 1, wherein the detection signal comprises at least one of: a plurality of liquid suction pipes (17) are arranged on the rotary liquid suction device (2).
5. The ELISA detector of claim 1, wherein the detection signal comprises at least one of: the blowing device (10) comprises a blowing pipe (18).
6. The ELISA detector of claim 1, wherein the detection signal comprises at least one of: and a switch gate (19) is arranged on the detection platform (1).
7. The ELISA detector of claim 1, wherein the detection signal comprises at least one of: the cuvette (7) is made of polyvinyl chloride.
8. The ELISA detector of claim 1, wherein the detection signal comprises at least one of: the bottom end of the detection table (1) is provided with a plurality of bottom cushions (20) and rollers (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921363041.1U CN211402399U (en) | 2019-08-21 | 2019-08-21 | Enzyme-linked immunoassay instrument for detecting beta-receptor stimulant |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921363041.1U CN211402399U (en) | 2019-08-21 | 2019-08-21 | Enzyme-linked immunoassay instrument for detecting beta-receptor stimulant |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115032151A (en) * | 2022-06-10 | 2022-09-09 | 杨富松 | Efficient multifunctional enzyme-labeling instrument and use method thereof |
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2019
- 2019-08-21 CN CN201921363041.1U patent/CN211402399U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115032151A (en) * | 2022-06-10 | 2022-09-09 | 杨富松 | Efficient multifunctional enzyme-labeling instrument and use method thereof |
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