CN211394473U

CN211394473U - Spirochete living body counting device

Info

Publication number: CN211394473U
Application number: CN201922067556.3U
Authority: CN
Inventors: 宝福凯; 李连保; 柳爱华; 陈泰桂; 罗丽莎; 徐鑫; 曹文静; 岳鹏; 张宇; 王峰
Original assignee: Kunming Medical University
Current assignee: Kunming Medical University
Priority date: 2019-11-26
Filing date: 2019-11-26
Publication date: 2020-09-01
Anticipated expiration: 2029-11-26

Abstract

The utility model discloses a spirochaeta living body counting device, which comprises a glass slide and a cover glass, wherein the glass slide is made of sepia optical glass, at least one counting cell is arranged on the glass slide, a square counting area is arranged in the middle of the counting cell, a circular arc groove-shaped sample adding and liquid filling area is arranged on one side edge of the counting cell, and the sample adding and liquid filling area is communicated with the counting cell; the cover glass is arranged on the upper part of the counting chamber. The utility model discloses the slide glass adopts and chooses brown optical glass for use, will lack in order to reduce light transmission amount, provides the dark field of vision, makes the spirochaeta can observe under ordinary optical microscope, and the utility model discloses can place and carry out the mirror examination under the microscope, the convenient minute object that needs the count to spirochaeta etc. of setting up through square count district counts.

Description

Spirochete living body counting device

Technical Field

The utility model relates to a microbiology and immunology laboratory glassware field especially relate to a spirochaeta live body counting assembly.

Background

In many microbial and immunological experiments, pathogenic microorganisms often need to be co-cultured with cells or tissues, and living pathogenic microorganisms need to be observed in order to determine the growth condition and the number of the pathogenic microorganisms.

At present, a dark field microscope is often needed for observing living spirochetes, but not all laboratories are equipped with the dark field microscope, how to observe and count the living spirochetes by using a common optical microscope becomes a problem, and counting and inspection are needed for the living spirochetes, the living spirochetes are generally scraped on glass slides and then covered with cover slips, so that the living spirochetes play a role in homogenizing sample tissues, liquid can be prevented from contacting with an objective lens to avoid polluting an objective lens, and cells to be observed can be positioned on the same plane at the top so as to be convenient for observation, comparison and counting. However, because the field of view of the glass slide and the cover glass under the microscope is large, for technical operation similar to various cells in blood, operators are fatigued, and even problems of repeated counting, missed technology and the like often occur, so that the counting is inaccurate, and the judgment of doctors on patients is influenced.

Therefore, it is an urgent need to solve the above-mentioned problems in the art to provide a spiral body counting apparatus which has a simple structure, can be used under a general optical microscope, and is convenient for counting the spiral body.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model provides a simple structure can use under ordinary optical microscope, is convenient for to spirochete live body counting assembly to spirochete live body count.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a spirochete living body counting device comprises a glass slide and a cover glass, wherein the glass slide is made of sepia optical glass, at least one counting cell is arranged on the glass slide, a square counting area is arranged in the middle of the counting cell, a circular arc groove-shaped sample adding and liquid filling area is arranged on one side edge of the counting cell, and the sample adding and liquid filling area is communicated with the counting cell;

the cover glass is arranged on the upper part of the counting chamber.

The utility model adopts the brown optical glass, which reduces the light transmission amount and provides dark field, so that the spirochete can be observed under a common optical microscope, and the utility model can be placed under the microscope for microscopic examination, and the micro objects such as the spirochete and the like which need to be counted can be counted conveniently through the arrangement of the square counting area; and the utility model discloses convenient the washing, simple to use can used repeatedly, has reduced the use consumption in laboratory.

The utility model discloses at first cover the cover glass when using, then application of sample liquid filling district adds the sample, and the sample can be filled the count pond automatically, then shift to ordinary optical microscope observe the count can, use portably.

Furthermore, the counting cell sinks by 0.1-0.15mm relative to the upper surface of the glass slide, the counting cell is rectangular, the length of the counting cell is 1-2mm, and the width of the counting cell is 1-2 mm.

By adopting the technical scheme, the counting chamber forms a space with a fixed volume on the glass slide, so that the counting chamber is convenient for bearing an experimental sample and observing and counting the spiral body.

Furthermore, the counting cells are two, the two counting cells are symmetrically arranged, and the sample adding and liquid filling area is relatively far away from the counting cells.

Adopt above-mentioned technical scheme, the utility model discloses when can guaranteeing to add the sample, can not cause contacting of two counting cells, avoid the result to interfere with each other.

Furthermore, the square counting area is arranged in the middle of the counting pool, and the separating line of the square counting area extends outwards to the edge of the counting pool.

Preferably, the specification of the square grid counting area is 5 × 5, and the square grid counting area has 25 big grids; the four corners and the middle large grid of the grid counting area are equally divided into 16 small grids of a rectangle.

Preferably, the specification of the square grid counting area is 5 × 5, the square grid counting area has 25 large grids, each large grid is divided into 16 small grids of a rectangle, and the total number of the small grids of the square grid counting area is 400 small squares.

The utility model discloses the square that can adopt two kinds of differences above counts respectively, and then can calculate comparatively accurate numerical value.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a schematic view of the overall structure of the spiral body living body counting device of the present invention;

FIG. 2 is an enlarged schematic view of a counting cell of the spiral body living body counting device of the present invention;

FIG. 3 is an enlarged structural diagram of a square counting area of the spiral body living body counting device of the present invention;

in the figure: 1 is a counting pool; 2 is a sample adding and liquid filling area; 3 is a cover glass; 4 is a grid counting area; and 5 is a glass slide.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1-3: a spirochaeta living body counting device comprises a glass slide 5 and a cover glass 3, wherein the glass slide 5 is made of sepia optical glass, at least one counting cell 1 is arranged on the glass slide, a square counting area 4 is arranged in the middle of the counting cell 1, a circular arc groove-shaped sample adding and liquid filling area 2 is arranged on one side edge of the counting cell 1, and the sample adding and liquid filling area 2 is communicated with the counting cell 1; the cover glass 3 is arranged at the upper part of the counting chamber 1.

The utility model discloses slide glass 5 adopts and chooses the brown optical glass for use, will reduce in order to reduce the light transmission amount, provide the dark field, make the spirochaeta can observe under ordinary optical microscope, and the utility model discloses can place and carry out the microscopic examination under the microscope, conveniently count the minute object that needs the count such as the spirochaeta through the setting of square count district 4; and the utility model discloses convenient the washing, simple to use can used repeatedly, has reduced the use consumption in laboratory.

The utility model discloses at first cover when using and put glass coverslip 3, then the application of sample liquid filling district 2 adds the sample, and the sample can be filled automatically and is full of counting cell 1, then shift to ordinary optical microscope observe the count can, it is simple and convenient to use.

In this embodiment, the counting chamber 1 is depressed by 0.1mm with respect to the upper surface of the slide 5, the counting chamber 1 is rectangular, and the counting chamber 1 has a length of 1mm and a width of 1 mm.

In other embodiments, the counting cell 1 is depressed by 0.1-0.15mm relative to the upper surface of the slide 5, the counting cell 1 is rectangular, and the counting cell 1 has a length of 1-2mm and a width of 1-2 mm.

The utility model discloses counting chamber 1 forms the space of a fixed volume on slide 5, is convenient for bear the weight of the experiment sample, is convenient for observe the count to the spiral body.

In this embodiment, the number of the counting cells 1 is two, and the two counting cells 1 are symmetrically arranged and the sample adding and liquid filling region 2 is relatively far away from the counting cells.

The utility model discloses when can guaranteeing to add the sample, can not cause contacting of two counting cells 1, avoid the result to interfere with each other.

In this embodiment, the square grid counting area 4 is disposed in the middle of the counting chamber 1, and the separating line of the square grid counting area 4 extends outward to the edge of the counting chamber 1.

In this embodiment, the standard of the square grid counting area 4 is 5 × 5, and there are 25 large grids; the four corners and the middle large grid of the grid counting area 4 are equally divided into 16 small grids of a rectangle.

In other embodiments, the square counting area 4 has a size of 5 × 5, and has 25 large grids, each large grid is divided into 16 small grids of a rectangle, and the total number of the small grids in the square counting area 4 is 400 small squares.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention.

Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A spirochete living body counting device comprises a glass slide (5) and a cover glass (3), and is characterized in that the glass slide (5) is made of sepia optical glass, at least one counting cell (1) is arranged on the glass slide, a grid counting area (4) is arranged in the counting cell (1), a circular arc groove-shaped sample adding and liquid charging area (2) is arranged on one side edge of the counting cell (1), and the sample adding and liquid charging area (2) is communicated with the counting cell (1);

the cover glass (3) is arranged on the upper part of the counting chamber (1).

2. A spiral body counting device according to claim 1, wherein the counting cell (1) is recessed 0.1-0.15mm with respect to the upper surface of the glass slide (5), the counting cell (1) is rectangular, and the counting cell (1) has a length of 1-2mm and a width of 1-2 mm.

3. The spiral body counting device according to claim 1, wherein the number of the counting cells (1) is two, the two counting cells (1) are symmetrically arranged, and the sample adding and liquid filling area (2) is relatively far away from the two counting cells.

4. A spiral body counting device according to claim 1, characterized in that the square counting area (4) is arranged in the middle of the counting cell (1), and the separation line of the square counting area (4) extends outwards to the edge of the counting cell (1).

5. A spiral body counting device according to any one of claims 2 to 4, wherein the size of the square counting area (4) is 5 x 5, and the number of the square counting area is 25; the four corners and the middle large grid of the grid counting area (4) are equally divided into 16 small grids of a rectangle.

6. A spiral body counting device according to any one of claims 2 to 4, wherein the size of the square counting area (4) is 5 x 5, the total number of the big grids is 25, each big grid is divided into 16 rectangular small grids, and the total number of the small grids of the square counting area (4) is 400 small grids.