CN218202822U - A plate culture ware for stereomicroscope spore count - Google Patents
A plate culture ware for stereomicroscope spore count Download PDFInfo
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- CN218202822U CN218202822U CN202221292637.9U CN202221292637U CN218202822U CN 218202822 U CN218202822 U CN 218202822U CN 202221292637 U CN202221292637 U CN 202221292637U CN 218202822 U CN218202822 U CN 218202822U
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- culture dish
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- stereomicroscope
- counting
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Abstract
The utility model relates to a counting assembly that biological field used specifically is a plate culture dish for stereomicroscope spore count, including the culture dish body and culture dish lid, the culture dish body is that the top has open-ended cuboid form, and the lateral wall of the culture dish body is the echelonment, including outer wall and inner wall, culture dish lid nestification is on the inner wall, and the outward flange flushes with the outer wall, and the bottom of the culture dish body is provided with the alcove, and the area of every alcove is 1cm 2 The flat culture dish is made of glassThe device is clear and transparent, is suitable for microscopic examination under a stereomicroscope, does not affect the collection of photos when detecting and counting spores, is easy to clean vessels made of glass materials, can be repeatedly used, saves resources, does not pollute the environment, has certain ecological effect, has a partition plate at the edge of the concave chamber, prevents the spores from drifting between the concave chambers, avoids remeasuring and missing of the spores, is convenient for a counter to stop recording when each small concave chamber is provided with different digital marks, and has the advantages of simple and convenient operation and strong practicability.
Description
Technical Field
The utility model relates to a counting assembly that biological field used specifically is a plate culture dish that is used for stereomicroscope spore count.
Background
Arbuscular Mycorrhizal Fungi (AMF) are beneficial soil microorganisms widely distributed in the terrestrial ecosystem and can be symbiotic with more than 90% of vascular plants to form reciprocal symbionts. The symbiosis of the AMF and the plants can not only promote the growth of the plants, but also enhance the adaptability of the plants to the environment and reduce the incidence rate. Meanwhile, saccamycin secreted by AMF can increase the organic carbon reservoir of soil and improve soil aggregates, and can be used for repairing degraded soil. Arbuscular mycorrhizal fungi have been favored by many scholars and experts in agriculture, forestry, animal husbandry and soil remediation. The AM fungal spore density is one of important parameters for measuring the definite value condition of the AM fungi in the soil, so that the vessel specially used for counting the AM fungal spores is designed and invented, a device with more accurate counting can be provided for students and experts researching the AM fungi, and convenience is provided for research work of the students and the experts.
The counting utensil under the existing common stereoscopic microscope is a traditional glass culture dish, the glass culture dish can meet the conventional requirements of microscopic examination under the stereoscopic microscope, but has the following defects when counting AM fungal spores: 1. the bottom of the traditional glass culture dish is not provided with scales and areas, the areas are not provided with corresponding marks, accurate counting is not facilitated during counting, re-counting and omission are easy to occur, and the marks are inconvenient to mark and stop during counting; 2. the bottom of a traditional glass culture dish is smooth and has no partition, and spores are easy to move during counting to cause re-counting and leakage counting of the number of the spores.
SUMMERY OF THE UTILITY MODEL
To solve the above technical problem, the utility model aims at providing a plate culture dish for stereomicroscope spore count. The specific technical scheme is disclosed as follows:
the utility model provides a plate culture dish for stereomicroscope spore count, plate culture dish include the culture dish body 1 and culture dish lid 3, culture dish body 1 is that the top has the open-ended cuboid form, the lateral wall of culture dish body 1 be the echelonment, including outer wall 101 and inner wall 102, inner wall 102 and outer wall 101 integrated into one piece, culture dish lid 3 nestification on inner wall 102, the outward flange flushes with outer wall 101, the bottom of culture dish body 1 be provided with alcove 2, the area of every alcove 2 is 1cm 2 And adding the spore liquid to be measured into the culture dish body 1, placing the culture dish body on a stereomicroscope objective table, then slightly shaking the plate culture dish, uniformly dispersing the spore liquid into each small concave chamber 2 with the digital marks, and then counting by a counter conveniently.
Preferably, the culture dish body 1 has an inner length of 10cm, an inner width of 10cm and an inner height of 2cm, and can be divided into 100 concave chambers 2 in half.
Preferably, the height of the inner wall 102 is 2cm, the width of the inner wall is 0.1cm, the width of the outer wall 101 is 0.1cm, the height of the outer wall is 1.6cm, and the height difference between the outer wall 101 and the inner wall 102 is convenient for nesting of the culture dish covers 3.
Preferably, the chambers 2 are formed by partitions 201, and the area of each chamber 2 is equal.
Preferably, the height of the partition board 201 is 0.2cm, so that spore liquid is conveniently and uniformly dispersed in each concave chamber, and spores can be prevented from drifting among the concave chambers due to the gentle movement of the dish body in the counting process.
Preferably, the bottom of the partition board 201 is provided with a number for a counter to count.
Preferably, the culture dish cover 3 is in a cuboid shape with an opening at the top, the inner length of the culture dish cover is 10.1cm, the inner width of the culture dish cover is 10.1cm, the outer length of the culture dish cover is 10.2cm, the outer width of the culture dish cover is 10.2cm, the height of the culture dish cover is more than or equal to 0.4cm, the culture dish cover is used for covering a dish body during non-counting, evaporation of detected spore liquid is reduced, and a common culture dish cover manufacturing method is adopted.
Compared with the prior art, the utility model discloses the technological effect that can reach includes:
1. the flat culture dish is made of glass, is clear and transparent, is suitable for microscopic examination under a stereoscopic microscope, does not affect the collection of photos when detecting and counting spores, is easy to clean, can be repeatedly used for many times, saves resources, does not pollute the environment, and has certain ecological effect.
2. Every little alcove border all has protruding baffle in this plate culture dish sample detection area, just with the even dispersion of spore liquid in every alcove, can prevent again that the spore that arouses because of the gentle removal of the ware body drifts between the alcove at the counting in-process, avoids weighing and leaking the meter of spore.
3. Different digital marks are designed in each small chamber in the sample detection area of the plate culture dish, stop and convenient recording are allowed for a counter, the occurrence of re-counting and missing counting of the small chamber are avoided, the operation is simple and convenient, and the practicability is high.
Drawings
FIG. 1 schematic view of a culture dish body 1
FIG. 2 schematic drawing of the culture dish lid 3
1. A culture dish body; 101. an outer wall; 102. an inner wall; 2. a recess; 201. a partition plate; 3. a culture dish cover; 301. upper wall
Detailed Description
The technical solution of the present invention is described in detail below with reference to the specific embodiments and the drawings, but it should be noted that the scope of the present invention is not limited by the following embodiments.
Example I A plate culture dish for counting spores of a stereomicroscope and a method of use
A plate culture dish for counting spores of a stereomicroscope comprises a culture dish body 1 and a culture dish cover 3, wherein the culture dish body 1 is in a cuboid shape with an opening at the top, the side wall of the culture dish body 1 is in a ladder shape and comprises an outer wall 101 and an inner wall 102, the inner wall 102 and the outer wall 101 are integrally formed,the culture dish cover 3 is nested on the inner wall 102, the outer edge of the culture dish cover is flush with the outer wall 101, the bottom of the culture dish body 1 is provided with concave chambers 2, and the area of each concave chamber 2 is 1cm 2 And adding the spore liquid to be measured into the culture dish body 1, placing the culture dish body on a stereomicroscope objective table, then slightly shaking the plate culture dish, uniformly dispersing the spore liquid into each small concave chamber 2 with the digital marks, and then counting by a counter conveniently. The culture dish body 1 is 10cm long, 10cm wide and 2cm high, and can be divided into 100 concave chambers 2 in an even mode. The height of the inner wall 102 is 2cm, the width of the inner wall is 0.1cm, the height of the outer wall 101 is 1.6cm, the height difference between the outer wall 101 and the inner wall 102 facilitates nesting of the culture dish cover 3, the concave chambers 2 are composed of partition plates 201, the area of each concave chamber 2 is equal, the height of each partition plate 201 is 0.2cm, spore liquid is conveniently and uniformly dispersed in each concave chamber, the movement of spores among the concave chambers caused by the gentle movement of the dish body in the counting process can be prevented, the bottoms of the partition plates 201 are provided with numbers to facilitate counting of a counter, the culture dish cover 3 is in a cuboid shape with an opening at the top, the inner length of the culture dish cover is 10.1cm, the inner width of the culture dish cover is 10.1cm, the outer length of the culture dish cover is 10.2cm, the outer width of the culture dish body is 10.2cm, the height of the culture dish cover is not less than 0.4cm, evaporation of the detected spore liquid is reduced, and the evaporation of the culture dish cover manufactured by using a common culture dish cover manufacturing method.
The using method comprises the following steps:
quantitatively placing the AM fungal spore solution to be counted in the culture dish body 1, placing the culture dish body on a stereomicroscope object stage, slightly shaking to uniformly disperse the liquid to be counted in each concave chamber 2, slowly moving the culture dish body 1, recording the number of spores of each concave chamber 2, and finally counting the sum of the number of spores of each concave chamber 2 to finish spore counting.
Claims (8)
1. The utility model provides a plate culture dish for stereomicroscope spore count, a serial communication port, plate culture dish include the culture dish body (1) and culture dish lid (3), the culture dish body (1) is the top and has open-ended cuboid form, the lateral wall of culture dish body (1) be the echelonment, including outer wall (101) and inner wall (102), inner wall (102) and outer wall (101) an organic wholeForming, the culture dish cover (3) is nested on the inner wall (102), the outer edge of the culture dish cover is flush with the outer wall (101), the bottom of the culture dish body (1) is provided with the concave chambers (2), and the area of each concave chamber (2) is 1cm 2 。
2. A plate culture dish according to claim 1, characterized in that the culture dish body (1) has an inner length of 10cm, an inner width of 10cm and an inner height of 2cm.
3. A plate culture dish according to claim 1, wherein the inner wall (102) has a height of 2cm and a width of 0.1cm, and the outer wall (101) has a width of 0.1cm and a height of 1.6cm.
4. A plate culture dish according to claim 1, wherein the well (2) is formed by a partition (201).
5. A plate culture dish according to claim 4, wherein the height of the partition (201) is 0.2cm.
6. A plate culture dish according to claim 4, characterized in that the bottom of the partition (201) is provided with a number.
7. The plate culture dish according to claim 1, wherein the dish lid (3) has a rectangular parallelepiped shape with an open top, an inner length of 10.1cm, an inner width of 10.1cm, an outer length of 10.2cm, an outer width of 10.2cm, and a height of 0.4cm or more.
8. A plate culture dish according to claim 1, characterized in that the culture dish body (1) and the culture dish lid (3) are made of glass material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221292637.9U CN218202822U (en) | 2022-05-27 | 2022-05-27 | A plate culture ware for stereomicroscope spore count |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221292637.9U CN218202822U (en) | 2022-05-27 | 2022-05-27 | A plate culture ware for stereomicroscope spore count |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218202822U true CN218202822U (en) | 2023-01-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221292637.9U Active CN218202822U (en) | 2022-05-27 | 2022-05-27 | A plate culture ware for stereomicroscope spore count |
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| Country | Link |
|---|---|
| CN (1) | CN218202822U (en) |
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- 2022-05-27 CN CN202221292637.9U patent/CN218202822U/en active Active
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