CN211263193U - Optical analysis device for detecting strip-shaped fluorescent signal - Google Patents
Optical analysis device for detecting strip-shaped fluorescent signal Download PDFInfo
- Publication number
- CN211263193U CN211263193U CN201922243530.XU CN201922243530U CN211263193U CN 211263193 U CN211263193 U CN 211263193U CN 201922243530 U CN201922243530 U CN 201922243530U CN 211263193 U CN211263193 U CN 211263193U
- Authority
- CN
- China
- Prior art keywords
- detection
- excitation light
- optical
- analysis device
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses an optical analysis device for rectangular shape fluorescence signal detects has solved traditional light path and has detected that there is the technical problem that sensitivity is low, repeatability is poor and background signal disturbs greatly in the rectangular shape fluorescence signal. The detection device comprises a detection circuit, an optical analysis device shell and an optical assembly, wherein the optical assembly is packaged in the optical analysis device shell and used for emitting an excitation light source and being located in the illumination direction of the excitation light source, the excitation light source is used for accurately projecting the excitation light to a detection line T of a detected object, and the fluorescence emitted by the detection line T is converged and then projected to the detection circuit. The utility model has simple structure, scientific and reasonable design and convenient use. The device improves the utilization rate of exciting light, increases the receiving amount of a receiving light path, controls the shape of an exciting light spot and reduces the interference of background signals, thereby improving the detection sensitivity and repeatability.
Description
Technical Field
The utility model relates to an optical analysis device for rectangular shape fluorescence signal detects.
Background
The fluorescence immunoassay instrument is an instrument for ultramicro analysis by using rare earth markers. The basic principle is that the antibody is marked by rare earth elements to prepare a reagent, a light source with a specific wavelength is used as exciting light for irradiation, the reagent is excited to emit fluorescence with another wavelength, and the fluorescence is optically analyzed to make diagnosis.
The optical analysis device of the existing fluorescence immunoassay instrument generally focuses the emitted light of the divergent point light source through the convex lens and irradiates the focused light on a measured object to form a small circular light spot. However, for a strip-shaped fluorescent signal formed by a test strip of a fluorescence immunochromatography method and a microfluidic chip of our company, the traditional light path is used for detection, and if a light spot is small, the light spot cannot cover the whole fluorescent signal area, so that the fluorescent signal is weak, and the fluorescent utilization rate is not high; if the light spot is large, the whole fluorescence signal area is covered, and meanwhile, excessive background signal areas are covered, so that the interference of background signals is increased. If a plurality of strip-shaped fluorescent signals are densely distributed, the traditional light path cannot accurately distinguish the required signals. That is to say, the traditional optical path detects the strip-shaped fluorescence signal, and has the defects of low sensitivity, poor repeatability and large interference of background signals.
Therefore, it is an urgent technical problem to be solved by those skilled in the art to design an optical analysis device for single or multiple strip fluorescent signal detection, which increases the receiving amount of the receiving light path by increasing the utilization rate of the excitation light, and controls the shape of the spot of the excitation light to reduce the interference of the background signal, thereby achieving the purpose of improving the detection sensitivity and repeatability.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is: the optical analysis device for detecting the strip-shaped fluorescent signal is provided, and the detection sensitivity and the repeatability are improved by improving the utilization rate of exciting light, increasing the receiving amount of a receiving light path and controlling the light spot shape of the exciting light to reduce the interference of background signals.
In order to achieve the above object, the utility model adopts the following technical scheme:
an optical analysis device for detecting a strip-shaped fluorescent signal comprises a detection circuit, an optical analysis device shell, an excitation light source and an optical assembly, wherein the excitation light source is packaged in the optical analysis device shell and used for emitting excitation light, the optical assembly is positioned in the illumination direction of the excitation light source and used for accurately projecting the excitation light onto a detection line T of a detected object, converging fluorescence emitted by the detection line T and projecting the converged fluorescence onto the detection circuit;
the optical assembly comprises an exciting light filter, a first convex lens and a dichroic mirror which are sequentially distributed along the direction of an exciting light path, a first cylindrical lens which is positioned on the reflection direction of the dichroic mirror and used for converging the exciting light reflected by the dichroic mirror into a long-strip-shaped light spot with the same shape and size as the detection line T, and a second convex lens and a fluorescent light filter which are sequentially distributed along the transmission direction of the dichroic mirror, the detection circuit is positioned right behind the fluorescent light filter, and the detection line T of the object to be detected is positioned on the light path of the long-strip-shaped light spot.
Further, a second cylindrical lens is arranged between the second convex lens and the fluorescent filter.
Further, an axial direction of the first cylindrical lens and an axial direction of the second cylindrical lens are perpendicular to each other.
Furthermore, the dichroic mirrors are distributed in an inclined mode, and an included angle of 45 degrees is formed between the dichroic mirrors and the horizontal plane.
Further, the optical analysis device housing comprises a left housing and a right housing which are matched with each other.
Further, an excitation light slit located on an excitation light path is formed between the light path-mounting left housing and the light path-mounting right housing, and the excitation light slit is located between the excitation light filter and the first convex lens.
Further, the detection circuit is fixedly mounted on the optical analysis device housing.
Further, the excitation light source is an LED point light source with a small emission angle.
Compared with the prior art, the utility model discloses following beneficial effect has:
the utility model has simple structure, scientific and reasonable design and convenient use. The device improves the utilization rate of exciting light, increases the receiving amount of a receiving light path, controls the shape of an exciting light spot and reduces the interference of background signals, thereby improving the detection sensitivity and repeatability.
The utility model discloses mainly form by the combination of the left casing of light path installation, excitation light source, detection circuitry, the right casing of light path installation and optical component. Detection line T on the measured object can be one also can be many evenly distributed, and rectangular shape facula does the utility model discloses the shape that optical analysis device shines on the measured object just covers the list fluorescence signal region. The intensity of each fluorescence signal can be detected in a scanning manner from strip to strip through the relative movement of the object to be detected and the optical analysis device; the optical component mainly comprises an excitation light filter, an excitation light slit, a first convex lens, a dichroic mirror, a first cylindrical lens, a second convex lens, a second cylindrical lens and a fluorescent light filter.
The utility model discloses the excitation light source that uses is the LED pointolite of little emission angle, the exciting light that this light source sent filters miscellaneous light through exciting light filter, the exciting light slit that the rethread was formed by left casing of light path installation and the right casing of light path installation blocks unnecessary exciting light, the exciting light through exciting light slit under the effect of assembling of first convex lens, let the exciting light tentatively assemble and shine to the dichroic mirror, under the reflection of dichroic mirror, exciting light shines downwards to first cylindrical lens, because first cylindrical lens has the folk prescription to assemble the effect to light, so last exciting light is assembled the surface of testee and is formed rectangular shape facula. Under the irradiation of the long-strip-shaped light spot, fluorescence is emitted by the detection line T, the fluorescence is firstly converged in a single direction by the first cylindrical lens, then the dichroic mirror is penetrated, and then the fluorescence is converged by the second convex lens, in order to enable the light converged to the detection circuit to be more, the light spot is smaller, the second cylindrical lens is arranged behind the second convex lens, the second cylindrical lens is axially vertical to the first cylindrical lens, the fluorescence is converged in another direction, and finally the fluorescence is irradiated onto the detection circuit after being filtered by the fluorescence filter, the detection circuit performs analysis and calculation, and finally a diagnosis result is obtained. The method has high detection sensitivity and good repeatability, and can effectively solve the technical problems of low sensitivity, poor repeatability and large interference of background signals in the conventional light path detection of the strip-shaped fluorescent signals.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic view of the object to be measured of the present invention.
Fig. 3 is the schematic structural diagram of the optical assembly in the front direction of the light path.
Fig. 4 is a schematic structural diagram of the optical assembly in the side direction of the optical path of the present invention.
Wherein, the names corresponding to the reference numbers are:
the device comprises a left shell, a 2-excitation light source, a 3-detection circuit, a right shell, a 5-optical component, a measured object, a 7-strip-shaped light spot, an 8-detection line T, a 9-quality control line C, a 10-excitation light filter, an 11-excitation light slit, a 12-first convex lens, a 13-dichroic mirror, a 14-first cylindrical lens, a 15-second convex lens, a 16-second cylindrical lens and a 17-fluorescence light filter, wherein the left shell is installed on a 1-light path, the right shell is installed on a 4-light path, and the fluorescence light filter is installed on a 6.
Detailed Description
The present invention will be further described with reference to the following description and examples, which include but are not limited to the following examples.
As shown in fig. 1-4, the utility model provides a pair of an optical analysis device for rectangular shape fluorescence signal detects, including detection circuitry 3, the optical analysis device casing, and all encapsulate in the optical analysis device casing is used for launching excitation light excitation source 2 and is located be used for projecting excitation light accurate on the detection line T8 to measured object 6 and projecting to after assembling the fluorescence that sends detection line T8 in the illumination direction of excitation light source 2 the optical component 5 of detection circuitry 3, detection circuitry 3 fixed mounting in on the optical analysis device casing, excitation light source 2 is the LED pointolite that has little emission angle. The optical analysis device shell comprises a left optical path mounting shell 1 and a right optical path mounting shell 4 which are matched with each other.
The utility model discloses light path that sends fluorescence at detection line T8 still is equipped with second cylindrical lens 16, first cylindrical lens 14 the axial with second cylindrical lens 16's axial mutually perpendicular, second cylindrical lens 16 are located second convex lens 15 with between fluorescence filter 17, fluorescence earlier by first cylindrical lens 14 carry out the folk prescription to assemble, it pierces through to carry out assembling of another direction by second cylindrical lens 16 to fluorescence after dichroic mirror 13, so can make and assemble the light on the detection circuitry 3 more, the facula is littleer.
The utility model has simple structure, scientific and reasonable design and convenient use. The device improves the utilization rate of exciting light, increases the receiving amount of a receiving light path, controls the shape of an exciting light spot and reduces the interference of background signals, thereby improving the detection sensitivity and repeatability. The technical problems of low sensitivity, poor repeatability and large interference of background signals in the conventional light path detection of the strip fluorescent signals can be effectively solved.
As shown in fig. 2, the utility model discloses the measured object 6 is detected the face and is gone up the distribution and have detection line T8 and quality control line C9, and detection line T8 is rectangular shape, and the shape size is unanimous with rectangular shape facula 7 mutually, and detection line T8's quantity is one or many, and when being more than one, all detection lines T8 equidistance distribute, and quality control line C9 is located measured object 6 one end on being detected the face for judge whether the measured object is effective.
The utility model discloses used detection circuitry 3 is current electrical equipment for carry out computational analysis and give the diagnosis result at last to the fluorescence facula that receives, its structure, circuit, theory of operation are prior art, consequently, do not give redundance here to detection circuitry 3's structure, circuit and theory of operation.
The utility model discloses mainly form by the combination of light path installation left side casing 1, excitation light source 2, detection circuitry 3, light path installation right side casing 4 and optical component 5. The object to be tested 6 is shown in fig. 2, wherein the detection line T8 can be one or more lines uniformly distributed, and the quality control line C9 is used for judging whether the object to be tested is effective or not. The long strip-shaped light spot 7 is the shape of the optical analysis device irradiated on the measured object 6, and just covers a single fluorescence signal area. The intensity of each fluorescent signal can be detected by scanning the object 6 relative to the optical analysis device. As shown in fig. 3 and 4, the optical assembly 5 is mainly composed of an excitation light filter 10, an excitation light slit 11, a first convex lens 12, a dichroic mirror 13, a first cylindrical lens 14, a second convex lens 15, a second cylindrical lens 16, and a fluorescence filter 17.
The utility model discloses the excitation light source 2 that uses is the LED pointolite of little emission angle, the exciting light that this light source sent filters miscellaneous light through exciting light filter 10, the exciting light slit 11 that is formed through left casing 1 of light path installation and light path installation right side casing 4 blocks unnecessary exciting light again, the exciting light through exciting light slit 11 is under the effect of assembling of first convex lens 12, let the exciting light tentatively assemble and shine to dichroic mirror 13, under dichroic mirror 13's reflection, exciting light shines downwards to first cylindrical lens 14, because first cylindrical lens 14 has the folk prescription to assemble the effect to light, so last exciting light is assembled the surface of testee 6 and is formed rectangular shape facula 7. Under the irradiation of the elongated light spot 7, the detection line T8 emits fluorescence, the fluorescence is converged in one direction by the first cylindrical lens 14, then the fluorescence passes through the dichroic mirror 13 and is converged by the second convex lens 15, in order to make the light converged to the detection circuit 3 more, the light spot is smaller, the second cylindrical lens 16 is arranged behind the second convex lens 15, the second cylindrical lens 16 is axially perpendicular to the first cylindrical lens 14, the fluorescence is converged in the other direction, and finally the fluorescence is filtered by the fluorescence filter 17 and then irradiated onto the detection circuit 3, the detection circuit 3 performs analysis and calculation, and finally a diagnosis result is obtained. The first cylindrical lens 14 and the second cylindrical lens 16 used in the present invention are both plano-convex cylindrical lenses.
The utility model has the advantages of simple structure and scientific and reasonable design, have sensitivity height and good reproducibility's characteristic, can effectively solve traditional light path and detect rectangular shape fluorescence signal and have sensitivity low, the repeatability is poor and the technical problem that the background signal disturbs greatly, its suitability is strong, is suitable for and widely popularizes and applies in this technical field.
The above embodiment is only one of the preferred embodiments of the present invention, and should not be used to limit the protection scope of the present invention, but all the insubstantial changes or modifications made in the spirit and the idea of the main design of the present invention, the technical problems solved by the embodiment are still consistent with the present invention, and all should be included in the protection scope of the present invention.
Claims (8)
1. An optical assay arrangement for the detection of elongate fluorescent signals, comprising a detection circuit (3), characterized in that: the device comprises an optical analysis device shell, an excitation light source (2) and an optical assembly (5), wherein the excitation light source (2) is packaged in the optical analysis device shell and used for emitting excitation light, the optical assembly (5) is positioned in the illumination direction of the excitation light source (2) and used for accurately projecting the excitation light to a detection line T (8) of a detected object (6), and the optical assembly is used for converging fluorescence emitted by the detection line T (8) and then projecting the fluorescence to the detection circuit (3);
optical assembly (5) are including exciting light filter (10), first convex lens (12) and dichroic mirror (13) along exciting light path direction distribution in proper order, are located be used for on the reflection direction of dichroic mirror (13) with exciting light that dichroic mirror (13) reflected assembles into first cylindrical lens (14) with the rectangular shape facula (7) that detection line T (8) shape size is the same, and along second convex lens (15) and fluorescence filter (17) that the transmission direction of dichroic mirror (13) distributes in proper order, detection circuitry (3) are located fluorescence filter (17) the dead astern, detection line T (8) of testee (6) are located the light path of rectangular shape facula (7).
2. An optical assay arrangement for the detection of elongate fluorescent signals according to claim 1, wherein: and a second cylindrical lens (16) is arranged between the second convex lens (15) and the fluorescent filter (17).
3. An optical assay arrangement for the detection of elongate fluorescent signals according to claim 2, wherein: the axial direction of the first cylindrical lens (14) and the axial direction of the second cylindrical lens (16) are perpendicular to each other.
4. An optical assay arrangement for the detection of elongate fluorescent signals according to claim 1, wherein: the dichroic mirrors (13) are distributed in an inclined mode, and form an included angle of 45 degrees with the horizontal plane.
5. An optical assay arrangement for the detection of elongate fluorescent signals according to claim 1, wherein: the optical analysis device shell comprises a left shell (1) and a right shell (4) which are matched with each other and are used for installing an optical path.
6. An optical assay arrangement for the detection of elongate fluorescent signals according to claim 5, wherein: an excitation light slit (11) located on an excitation light path is formed between the light path installation left casing (1) and the light path installation right casing (4), and the excitation light slit (11) is located between the excitation light filter (10) and the first convex lens (12).
7. An optical assay arrangement for the detection of elongate fluorescent signals according to claim 1, wherein: the detection circuit (3) is fixedly arranged on the optical analysis device shell.
8. An optical assay arrangement for the detection of elongate fluorescent signals according to claim 1, wherein: the excitation light source (2) is an LED point light source with a small emission angle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922243530.XU CN211263193U (en) | 2019-12-13 | 2019-12-13 | Optical analysis device for detecting strip-shaped fluorescent signal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922243530.XU CN211263193U (en) | 2019-12-13 | 2019-12-13 | Optical analysis device for detecting strip-shaped fluorescent signal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211263193U true CN211263193U (en) | 2020-08-14 |
Family
ID=71986840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922243530.XU Active CN211263193U (en) | 2019-12-13 | 2019-12-13 | Optical analysis device for detecting strip-shaped fluorescent signal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211263193U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113155801A (en) * | 2021-05-10 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Fluorescence detector |
| CN117929345A (en) * | 2024-03-22 | 2024-04-26 | 北京万泰生物药业股份有限公司 | Handheld detection device and fluorescence detection system |
-
2019
- 2019-12-13 CN CN201922243530.XU patent/CN211263193U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113155801A (en) * | 2021-05-10 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Fluorescence detector |
| CN113155801B (en) * | 2021-05-10 | 2023-02-03 | 新羿制造科技（北京）有限公司 | Fluorescence detector |
| CN117929345A (en) * | 2024-03-22 | 2024-04-26 | 北京万泰生物药业股份有限公司 | Handheld detection device and fluorescence detection system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5315375A (en) | Sensitive light detection system | |
| CN201514380U (en) | Device for quickly detecting protein content in liquid samples based on fluorescent technique | |
| CN211263193U (en) | Optical analysis device for detecting strip-shaped fluorescent signal | |
| CN101178399B (en) | Method for analyzing a sample on a test element and analysis system | |
| CN110308127A (en) | Can more fluorescence signals simultaneously detect optical assay device | |
| US20090059207A1 (en) | Method and device for measuring photoluminescence, absorption and diffraction of microscopic objects in a fluid | |
| CN109690293A (en) | System and method for time-resolved fluoroimmunoassay detection | |
| CN101705280A (en) | Method and device for quantitative PCR multi-wavelength fluorescence detection | |
| CN106969833B (en) | A kind of the ultraviolet detector system and its application of big visual field | |
| CN110865062A (en) | Optical analysis device for detecting strip-shaped fluorescent signal | |
| CN104880450A (en) | Line focusing detection system for immunofluorescence reagent card | |
| CN107264474A (en) | Rain sensor with multiple sensitivity areas | |
| CN207964840U (en) | The food safety comprehensive analytical equipment of fluorescence immune chromatography quantitative check can be achieved | |
| CN219285203U (en) | Fluorescent immunoassay instrument | |
| CN115980333A (en) | Fluorescence immunoassay method and optical system | |
| SU1276268A3 (en) | Method for checking transparent material strip for defects and device for effecting same | |
| US20060281190A1 (en) | Analyte detection using luminescence concentration | |
| CN104965078A (en) | Multi-channel fluorescence immunoassay quantitative analyzer | |
| CN211061419U (en) | Optical system and detector | |
| CN205120591U (en) | Burnt signal pickup assembly of fluorescence copolymerization | |
| CN204389396U (en) | Fluorescence detection device | |
| US20150292940A1 (en) | Photodetection apparatus | |
| CN212722627U (en) | Test paper strip color development detection device | |
| JP2002139439A (en) | Diffused light source for inspection | |
| CN206648953U (en) | A kind of fluorimetric analysis device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |