CN211255875U

CN211255875U - PCR rapid detection experimental box

Info

Publication number: CN211255875U
Application number: CN201921220659.2U
Authority: CN
Inventors: 梁伟能; 刘日威; 张冠文; 刘科勇; 倪树标; 曾成柱; 卢雅琳; 陈云
Original assignee: Guangzhou Lanfeng Technology Co ltd; Guangdong Institute Of Analysis (china National Analytical Center Guangzhou)
Current assignee: Guangzhou Lanfeng Technology Co ltd; Institute Of Testing And Analysis Guangdong Academy Of Sciences Guangzhou Analysis And Testing Center China
Priority date: 2019-07-29
Filing date: 2019-07-29
Publication date: 2020-08-14
Anticipated expiration: 2029-07-29

Abstract

The utility model discloses a PCR short-term test experimental box. This PCR short-term test experimental box, the power distribution box comprises a box body, be provided with the board of placing fluorescence PCR appearance in the box, ultraviolet lamp and first filter equipment, place the board and set up in the bottom of box, the ultraviolet lamp is fixed in the top of box, first filter equipment sets up in the inboard of fan, the fan sets up on the inner wall of box, be provided with the gas outlet on the inner wall of box one side of fan, first filter equipment top is provided with the drying cabinet, first filter equipment below is provided with the power supply module, the isolated power source interface of power supply module for setting up on the box inner wall, be provided with incasement instrument socket in the box, be provided with the air inlet on the opposite side inner wall of box, the air inlet. The utility model provides a PCR short-term test experimental box low cost and quick realization PCR expansion district prevent that the result of amplification detection stage from leaking the experimental box and causing the pollution outward.

Description

PCR rapid detection experimental box

The technical field is as follows:

the utility model belongs to the technical field of biological detection, concretely relates to PCR short-term test experimental box.

Background art:

PCR (polymerase chain reaction) detection technique A DNA amplification detection technique that simulates the process of DNA replication in vitro can perform exponential amplification on target DNA, thereby improving detection sensitivity. However, due to its extremely high amplification capacity, trace contamination can lead to false positives, and severe contamination can even lead to downtime of the entire laboratory. Thus, PCR laboratories typically require compartmentalization, i.e., a reagent preparation area, a sample preparation area, and a PCR amplification area. The PCR amplification region is the main source of contamination and should be under negative pressure to ensure that the DNA-containing aerosol diffuses out.

However, the PCR detection technology is gradually applied to various rapid detection occasions, and more rapid detection laboratories do not have conditions for realizing PCR partitioning.

The utility model has the following contents:

an object of the utility model is to provide a PCR short-term test experimental box solves the problem that prior art exists, realizes a simple and easy PCR amplification district, reaches and prevents the experiment and pollutes, satisfies the requirement of PCR short-term test experiment.

The utility model aims at providing a PCR rapid detection experimental box, which comprises a box body, wherein a placing plate for placing a fluorescent PCR instrument, an ultraviolet lamp and a first filtering device are arranged in the box body, the placing plate is arranged at the bottom of the box body, the ultraviolet lamp is fixed at the top of the box body, the first filtering device is arranged at the inner side of the fan, the fan is arranged on the inner wall of the box body, an air outlet is arranged on the inner wall of one side of the box body provided with the fan, a drying box is arranged above the first filtering device, a power supply module is arranged below the first filtering device, the power supply module is an isolated power interface arranged on the inner wall of the box body, an instrument socket in the box body is arranged in the box body, and an air inlet is formed in the inner wall of the other side of the box body, and a second filtering device is arranged on the inner side of the air inlet. The power supply module provides voltage for instruments in the experimental box, and the replaceable drying bag is placed in the drying box to provide a proper working environment for the instruments in the experimental box.

The utility model discloses a working process is: when the device is used, the fluorescence PCR instrument is placed in the box body, the power supply in the box body is connected, the isolated power supply of the box body is started, and the negative pressure generating device is started (namely, the fan is started to draw out air in the box body through the air outlet so as to form a negative pressure state in the box body); during detection, the fluorescence PCR instrument is arranged, the prepared PCR tube is placed into the fluorescence PCR instrument, the fluorescence PCR instrument is opened to start detection, and the door of the detection box body is tightly closed. After the detection is finished, the ultraviolet lamp is turned on to observe whether the fluorescence phenomenon exists. If a fluorescence phenomenon is found, indicating that aerosol leakage pollutes, ensuring that a box door of the detection box body is closed, closing a power supply in the box body, and immediately carrying the whole experimental box out of a laboratory for cleaning treatment; if no fluorescence phenomenon is found, the box door can be opened, the detection result can be recorded, and the experimental PCR tube can be removed. After the experiment is completed each time, the door of the experiment box is immediately closed, and the ultraviolet lamp is opened for disinfection treatment.

Preferably, the box body is provided with a box door, the box door is fixedly connected with the box body, and a sealing ring is arranged between the box door and the box body. The box door is hinged with the box body or connected with the box body through a hinge. The box door is arranged to facilitate the convenient taking out or putting in of the instrument in the experimental box during detection. The box door and the box body are arranged in a sealing mode, and the sealing environment during PCR detection is guaranteed.

Preferably, the outer side of the box door is provided with a handle for pulling the box door open, and the inner side of the box door is provided with a door lock. The handle is provided to facilitate opening of the door by a detected operator.

Preferably, the bottom of the box body is uniformly provided with non-slip pads, and the bottom of each non-slip pad is provided with non-slip lines. The non-slip mat prevents the experiment box from sliding randomly on the operation desktop, and ensures the smooth proceeding of PCR detection. The anti-slip lines can be stripe anti-slip lines, stripe anti-slip lines transversely and longitudinally staggered or fingerprint-shaped anti-slip lines, the distance between every two adjacent fingerprint-shaped anti-slip lines is 3-5 mm, and the anti-slip effect of the fingerprint-shaped anti-slip lines is optimal.

The utility model has the advantages that:

1. the PCR rapid detection experiment box provided by the utility model realizes PCR expansion area with low cost and rapidly, and prevents the product in the amplification detection stage from leaking outside the experiment box to cause pollution;

2. the ultraviolet lamp is arranged in the experimental box, so that the disinfection and the damage to the pollution of amplification products in the experimental box can be realized; meanwhile, the experimental box is also provided with a drying box, so that a good working environment can be provided for instruments in the experimental box body.

Description of the drawings:

FIG. 1 is a schematic structural diagram of the PCR rapid detection experiment box of the present invention;

description of reference numerals: 1. an air inlet; 2. a fluorescent PCR instrument; 3. a box body; 4. an ultraviolet lamp; 5. a drying oven; 6. a fan; 7. a first filtering device; 8. an isolated power interface; 9. an ultraviolet lamp switch; 10. an in-cabinet instrument receptacle; 11. a seal ring; 12. a box door; 13. a door lock is provided.

The specific implementation mode is as follows:

the following examples are further illustrative of the present invention and are not intended to be limiting thereof.

In the description of the present invention, it is to be understood that the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit indicating the number of technical features indicated, whereby the features defined as "first", "second", etc. may explicitly or implicitly include at least one such feature.

Example 1:

as shown in FIG. 1, a PCR rapid detection experimental box comprises a box body 3, a placing plate for placing a fluorescent PCR instrument 2 is arranged in the box body 3, ultraviolet lamp 4 and first filter equipment 7, place the board and set up in the bottom of box 3, it is provided with fluorescence PCR appearance 2 to place on the board, ultraviolet lamp 4 is fixed in the top of box 3, first filter equipment 7 sets up in the inboard of fan 6, fan 6 sets up on the inner wall of box 3, be provided with the gas outlet on the 3 one side inner walls of box that are provided with

fan

6, 7 tops of first filter equipment are provided with

drying cabinet

5, 7 belows in first filter equipment are provided with the power supply module, the power supply module is for setting up the isolated power source 8 on the 3 inner walls of box, be provided with incasement instrument socket 10 in the box 3, be provided with air inlet 1 on the opposite side inner wall of

box

3, 1 inboard in air inlet is provided with second filter. The power supply module provides voltage for instruments in the experimental box, and the replaceable drying bag is placed in the drying box 5 to provide a proper working environment for the instruments in the experimental box.

In this embodiment, the first filter device and the second filter device are both HEPA high efficiency filters, and the first filter device is used for preventing DNA generated in the detection amplification process from leaking out of the laboratory box and polluting other areas in the laboratory. The second filter device serves to prevent external contaminants from entering the laboratory box.

The box body 3 is provided with a box door 12, the box door 12 is fixedly connected with the box body 3, and a sealing ring 11 is arranged between the box door 12 and the box body 3. Door 12 is hingedly attached to cabinet 3 or by hinges, and in this embodiment door 12 is preferably hingedly attached to cabinet 3. The door 12 is provided to facilitate the easy removal and insertion of the laboratory instruments during testing. The box door 12 and the box body 3 are arranged in a sealing mode, and a sealing environment during PCR detection is guaranteed.

The outer side of the box door 12 is provided with a handle for pulling the box door 12 open, and the inner side of the box door 12 is provided with a door lock 13. The handle is provided to facilitate opening of the door 12 by a detecting operator.

The bottom of the box body 3 is evenly provided with non-slip mats, and the bottom of the non-slip mats is provided with non-slip lines. The non-slip mat prevents the experiment box from sliding randomly on the operation desktop, and ensures the smooth proceeding of PCR detection. The anti-slip lines can be stripe anti-slip lines, stripe anti-slip lines transversely and longitudinally staggered or fingerprint-shaped anti-slip lines, the distance between every two adjacent fingerprint-shaped anti-slip lines is 3-5 mm, and the anti-slip effect of the fingerprint-shaped anti-slip lines is optimal.

The utility model discloses a working process is: when the device is used, the fluorescent PCR instrument 2 is placed on a placing plate in the box body 3, an in-box power supply is connected, an isolated power supply of the box body is started, and the negative pressure generating device is started (namely, the fan is started to pump out air in the box body through the air outlet so as to form a negative pressure state in the box body); during detection, the fluorescent PCR instrument 2 is arranged, the prepared PCR tube is placed into the fluorescent PCR instrument 2, the fluorescent PCR instrument 2 is opened to start detection, and the door of the detection box body is tightly closed. After the detection is finished, the ultraviolet lamp 4 is turned on through the ultraviolet lamp switch 9, and whether the fluorescence phenomenon exists or not is observed. If a fluorescence phenomenon is found, indicating that aerosol leakage pollutes, ensuring that a box door of the detection box body is closed, closing a power supply in the box body, and immediately carrying the whole experimental box out of a laboratory for cleaning treatment; if no fluorescence phenomenon is found, the box door can be opened, the detection result can be recorded, and the experimental PCR tube can be removed. After the experiment is completed each time, the door of the experiment box is immediately closed, and the ultraviolet lamp is opened for disinfection treatment.

The above is to the utility model provides a PCR short-term test experimental box has carried out detailed introduction, and the description of above embodiment is only used for helping understanding the utility model discloses a technical scheme and core thought thereof should point out, to technical personnel in this technical field, under the prerequisite that does not deviate from the utility model discloses the principle, can also be right the utility model discloses carry out a plurality of improvements and embellishment, these improvements and embellishment also fall into the protection scope of the utility model claims.

Claims

1. A PCR rapid detection experimental box is characterized by comprising a box body, wherein a placing plate for placing a fluorescent PCR instrument, an ultraviolet lamp and a first filtering device are arranged in the box body, the placing plate is arranged at the bottom of the box body, the ultraviolet lamp is fixed at the top of the box body, the first filtering device is arranged at the inner side of the fan, the fan is arranged on the inner wall of the box body, an air outlet is arranged on the inner wall of one side of the box body provided with the fan, a drying box is arranged above the first filtering device, a power supply module is arranged below the first filtering device, the power supply module is an isolated power interface arranged on the inner wall of the box body, an instrument socket in the box body is arranged in the box body, and an air inlet is formed in the inner wall of the other side of the box body, and a second filtering device is arranged on the inner side of the air inlet.

2. The PCR rapid detection experimental box of claim 1, wherein a box door is arranged on the box body, the box door is fixedly connected with the box body, and a sealing ring is arranged between the box door and the box body.

3. The PCR rapid detection experimental box of claim 2, wherein a handle for opening the box door is arranged on the outer side of the box door, and a door lock is arranged on the inner side of the box door.

4. The PCR rapid detection experiment box of claim 1, wherein the bottom of the box body is uniformly provided with non-slip pads, and the bottom of the non-slip pads is provided with non-slip lines.