CN218401710U

CN218401710U - Fluorescence quantitative PCR detection kit for pathogenic bacteria

Info

Publication number: CN218401710U
Application number: CN202222925010.9U
Authority: CN
Inventors: 刘奕; 张国宁; 靖相密
Original assignee: Shandong Acv Biotech Co ltd
Current assignee: Shandong Acv Biotech Co ltd
Priority date: 2022-11-03
Filing date: 2022-11-03
Publication date: 2023-01-31
Anticipated expiration: 2032-11-03

Abstract

The scope of the invention is defined by the appended claims and equivalents thereof. The utility model relates to a kit technical field, concretely relates to fluorescence quantitative PCR detect reagent box of pathogenic bacterium. The utility model provides a fluorescence quantitative PCR detection kit for pathogenic bacteria, which can open a box cover in a targeted manner, and comprises a placing box, a box cover, a baffle, a fixed plate, a placing rack, a pulling block and the like; place the box top and be provided with the lid, the inside baffle that is equipped with a plurality of rotatable formulas of lid is provided with on the baffle and draws the piece, and the lid upper end is provided with the fixed plate that is used for fixed stop, places the box inside and is the fixed three rack that is provided with, and it has a plurality of holes of placing that are used for fixed test tube to open on the rack. When using this device, only need draw the baffle that the piece drove to correspond through the pulling, owing to not opening whole lid, consequently when opening the baffle, the test tube of other parts can not be influenced to potential pollution sources in the external environment.

Description

Fluorescence quantitative PCR detection kit for pathogenic bacteria

Technical Field

The utility model relates to a kit technical field especially relates to a pathogenic bacterium fluorescence quantitative PCR detect reagent box.

Background

The fluorescent quantitative PCR detection kit is a product for detecting nucleic acid fragments in a biological sample by adopting a fluorescent quantitative PCR technology, and generally comprises a reaction solution, an enzyme mixed solution, a reagent such as RNA enzyme removing water and the like, a reagent tube and a packing box. The fluorescence quantitative PCR is a method for quantitatively analyzing a specific DNA sequence in a sample to be detected by an internal reference or external reference method by measuring the total amount of products after each Polymerase Chain Reaction (PCR) cycle by using a fluorescent chemical substance in a DNA amplification reaction. The method comprises the steps of marking and tracking a PCR product through a fluorescent dye or a fluorescent-marked specific probe, monitoring the whole PCR process in real time by utilizing fluorescent signal accumulation, and finally calculating the initial concentration of a sample template to be detected through a standard curve. Has been widely applied to the fields of rapid diagnosis of pathogenic microorganisms such as bacteria, viruses, mycoplasma and the like, genetic disease gene detection, tumor gene detection, food health quarantine and the like.

Most of the existing fluorescence quantitative PCR detection kits for pathogenic bacteria are single-fold or double-fold detection, namely, only 1-2 pathogenic bacteria can be detected at one time, and a few products can detect more than three bacteria. When the kit is used, the kit needs to be opened, and a certain amount of reagent is sucked by a liquid-transferring gun for a detection experiment. In the process, the box body of the kit is completely opened, so that pollution is easily caused, and great deviation is caused to an experimental result.

Therefore, it is necessary to design a fluorescent quantitative PCR detection kit capable of simultaneously detecting a plurality of bacteria while avoiding external contamination (microorganisms, amplification products, etc. in the environment).

SUMMERY OF THE UTILITY MODEL

In order to overcome the in-process that detects the sample, all open the lid and cause the shortcoming of other test tubes of external pollution easily, the utility model provides a can have corresponding pathogenic bacterium fluorescence quantitative PCR detect reagent box of opening the lid.

The utility model provides a pathogenic bacterium fluorescence ration PCR detect reagent box, is including placing box, lid, baffle, fixed plate, rack and drawing the piece, places the box top and is provided with a lid, and the inside baffle that is equipped with a plurality of rotatable formulas of lid is provided with on the baffle and draws the piece, and the lid upper end is provided with the fixed plate that is used for fixed stop, places the box inside and is the fixed a plurality of racks that are provided with, and it has a plurality of holes of placing that are used for fixed test tube to open on the rack.

Furthermore, the device also comprises an observation film, and the observation film is arranged around the placing box.

Furthermore, the device also comprises a placing cylinder and a rolling wheel, wherein the middle part of the placing box is provided with the placing cylinder, and the rolling wheel is arranged in the placing cylinder.

Furthermore, the rack device also comprises a partition plate, and the partition plate is arranged between every two adjacent placing racks.

Furthermore, the box also comprises a limiting plate, wherein a round limiting plate is arranged at the top end inside the placing box, and the size of the limiting plate is matched with the inner circumference of the placing box.

The utility model discloses the beneficial effect who has as follows:

1. when using this device, only need slide the baffle that corresponds, because not open whole lid, consequently when opening the baffle, the test tube of other parts is hardly influenced to potential pollution sources in the external environment, has reached and has avoided the pollution, the effect of guarantee experimental result accuracy.

2. Observe the film and can effectually observe placing the inside test tube of box, conveniently take notes the experimentation.

3. Place the limiting plate that box upper end set up, effectually prevented to place the inside test tube of box and take place to rock and then lead to the condition that the test tube dropped at the removal in-process, played the effect of protection to the test tube.

Drawings

Fig. 1 is a schematic view of the three-dimensional structure of the present invention.

Fig. 2 is a schematic view of the three-dimensional structure of the components such as the placing cylinder and the rolling wheel of the present invention.

Fig. 3 is a schematic view of the three-dimensional structure of the observation film and the partition board.

Fig. 4 is a schematic perspective view of the baffle plate and the fixing plate of the present invention.

In the above drawings: 1: placing box, 2: a box cover, 201: baffle, 202: fixing plate, 3: rack, 4: placement cylinder, 41: reel, 5: placing hole, 6: observation of the film, 7: pulling block, 8: limiting plate, 9: a partition plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1

The utility model provides a pathogenic bacteria fluorescence quantitative PCR detect reagent box, as shown in figure 1, figure 2 and figure 4, including placing box 1, lid 2, baffle 201, fixed plate 202, rack 3 and drawing piece 7, it is provided with a lid 2 to place 1 top of box, 2 inside baffle 201 that are equipped with three rotatable formula of lid, be provided with on the baffle 201 and draw piece 7, 2 upper ends of lid are provided with a fixed plate 202 that is used for fixed baffle 201, it is fixed three rack 3 that is provided with to place 1 inside of box, it has a plurality of holes 5 of placing that are used for fixed test tube to open on the rack 3.

When reagent pipe is placed to needs when placing 1 inside of box, only need pulling the piece 7 that corresponds, pull piece 7 and drive baffle 201, insert reagent pipe afterwards place the hole 5 inside can, owing to only opened baffle 201, not take off whole lid 2, consequently can not influence the test tube of other parts, the fixed plate 202 that lid 2 upper end set up can effectively be fixed baffle 201, prevent that the condition of dislocation from appearing at the in-process that removes baffle 201.

Example 2

In addition to the embodiment 1, as shown in fig. 3, the container further includes an observation film 6, and the observation film 6 is uniformly spaced around the placing case 1.

Observe film 6 and can observe the test tube in the box, conveniently at any time to the test tube in the experimental conditions record.

As shown in fig. 2, the box further comprises a placing barrel 4 and a rolling wheel 41, the placing barrel 4 is arranged in the middle of the placing box 1, the rolling wheel 41 is arranged in the placing barrel 4, and the placing box is fixedly connected with the bottom of the placing box 1.

The reel 41 in the placing barrel 4 is wound with a test tube label, so that the test tube is conveniently marked.

As shown in fig. 3, the rack further comprises a partition plate 9, and a partition plate 9 is arranged between every two adjacent racks 3.

Division board 9 in the box can carry out the separation to the bacterium that comes from the external world, prevents to influence inside test tube because of the bacterium to cause the inaccurate condition of experimental result.

As shown in fig. 3, the box further comprises a limiting plate 8, a circular limiting plate 8 is arranged at the top end inside the placing box 1, and the size of the limiting plate 8 is matched with the circumference inside the placing box 1.

Place limiting plate 8 that the inside top of box 1 set up and can effectively carry on spacingly to the test tube, prevent to appear because of placing the condition that the in vitro culture solution that box 1 removed and lead to spills.

The above-mentioned embodiments only represent the preferred embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several changes, modifications and substitutions can be made, which are all within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. The utility model provides a pathogenic bacterium fluorescence quantitative PCR detect reagent box, is provided with a lid including placing box, lid, placing box top, its characterized in that still including baffle, fixed plate, rack and pull the piece, the inside baffle that is equipped with a plurality of rotatable formulas of lid is provided with the pull piece on the baffle, and the lid upper end is provided with the fixed plate that is used for fixed stop, places box inside and is fixed a plurality of racks that are provided with, and it has a plurality of holes of placing that are used for fixed test tube to open on the rack.

2. The fluorescence quantitative PCR detection kit for pathogenic bacteria according to claim 1, wherein: the device also comprises an observation film, and the observation film is arranged around the placing box.

3. The fluorescence quantitative PCR detection kit for pathogenic bacteria according to claim 2, wherein: the device is characterized by further comprising a placing barrel and a winding wheel, wherein the placing barrel is arranged in the middle of the placing box, and the winding wheel is arranged in the placing barrel.

4. The fluorescence quantitative PCR detection kit for pathogenic bacteria according to claim 3, wherein: the rack is characterized by further comprising partition plates, and the partition plates are arranged between every two adjacent placing racks.

5. The fluorescence quantitative PCR detection kit for pathogenic bacteria according to claim 4, wherein: the box is characterized by further comprising a limiting plate, wherein the round limiting plate is arranged at the top end inside the placing box, and the size of the limiting plate is matched with the inner circumference of the placing box.